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100%;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section style=\"display: contents;\" powered-by=\"xiumi.us\">\n<section style=\"display: contents;\">\n<section style=\"display: contents;\">\n<section style=\"height: 0px;overflow: hidden;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;caret-color: red;\"> <\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 10px 0% 8px;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-left: 3px solid rgb(219, 219, 219);border-bottom-left-radius: 0px;padding-left: 8px;align-self: 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\uff0c\u8868\u793a\u4e24\u7ec4\u6837\u54c1\u4e4b\u95f4\u7684\u5dee\u5f02\u500d\u6570\uff0c\u70b9\u8d8a\u504f\u79bb\u4e2d\u5fc3\uff0c\u8868\u793a\u5dee\u5f02\u500d\u6570\u8d8a\u5927\u3002\u706b\u5c71\u56fe\u7684\u7eb5\u8f74\u4e00\u822c\u662f -Log 10 (P-value) \uff0c\u8868\u793a\u5dee\u5f02\u662f\u5426\u5177\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\uff0c\u70b9\u8d8a\u9760\u56fe\u7684\u9876\u90e8\u8868\u793a\u5dee\u5f02\u8d8a\u663e\u8457\u3002\u706b\u5c71\u56fe\u7684\u70b9\u7684\u989c\u8272\u548c\u5927\u5c0f\u4e5f\u53ef\u4ee5\u8868\u793a\u66f4\u591a\u7684\u5c5e\u6027\uff0c\u5982\u57fa\u56e0\u7684\u4e0a\u8c03\u3001\u4e0b\u8c03\u3001\u65e0\u5dee\u5f02\u3001\u8868\u8fbe\u4e30\u5ea6\u7b49\u3002\u706b\u5c71\u56fe\u53ef\u4ee5\u5e2e\u52a9\u6211\u4eec\u5feb\u901f\u7b5b\u9009\u51fa\u5177\u6709\u751f\u7269\u5b66\u610f\u4e49\u7684\u5dee\u5f02\u57fa\u56e0\uff0c\u5e76\u5728\u56fe\u4e0a\u6807\u8bb0\u51fa\u6765\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4ecb\u7ecd\u5b8c\u4e86\u706b\u5c71\u56fe\uff0c\u4e3a\u4e86\u5c0f\u4f19\u4f34\u4eec\u66f4\u52a0\u5168\u9762\u7684\u8ba4\u8bc6\u706b\u5c71\u56fe\uff0c\u4e0b\u9762\u5c0f\u679c\u8bf4\u4e00\u4e0b\u5b83\u7684\u4f18\u7f3a\u70b9\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"font-size: 14px;\">\u706b\u5c71\u56fe\u7684\u4f18\u70b9\uff1a<\/span><\/strong><\/p>\n<\/section>\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"border-width: 1px;border-style: solid;border-color: black;padding: 3px;\">\n<section style=\"border-color: black;border-width: 3px;border-style: solid;padding: 10px;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\">1. \u5b83\u53ef\u4ee5\u5feb\u901f\u76f4\u89c2\u5730\u5c55\u793a\u51fa\u53d8\u5316\u5e45\u5ea6\u8f83\u5927\u4e14\u5177\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\u7684\u6570\u636e\u70b9\uff0c\u5982\u5dee\u5f02\u57fa\u56e0\u7b49<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\">2. \u5b83\u53ef\u4ee5\u5728\u56fe\u4e0a\u6807\u8bb0\u51fa\u5173\u6ce8\u7684\u57fa\u56e0\u7684\u540d\u5b57\uff0c\u65b9\u4fbf\u540e\u7eed\u7684\u5206\u6790\u548c\u9a8c\u8bc1<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\">3. \u5b83\u53ef\u4ee5\u7528\u70b9\u7684\u989c\u8272\u548c\u5927\u5c0f\u8868\u793a\u66f4\u591a\u7684\u5c5e\u6027\uff0c\u5982\u57fa\u56e0\u7684\u4e0a\u8c03\u3001\u4e0b\u8c03\u3001\u8868\u8fbe\u4e30\u5ea6\u7b49<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"font-size: 14px;\">\u706b\u5c71\u56fe\u7684\u7f3a\u70b9\uff1a<\/span><\/strong><\/p>\n<\/section>\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"border-width: 1px;border-style: solid;border-color: black;padding: 3px;\">\n<section style=\"border-color: black;border-width: 3px;border-style: solid;padding: 10px;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\">1. \u5b83\u53ea\u9002\u5408\u5c55\u793a\u4e24\u7ec4\u6837\u54c1\u4e4b\u95f4\u7684\u6bd4\u8f83\uff0c\u4e0d\u80fd\u5c55\u793a\u591a\u7ec4\u6837\u54c1\u4e4b\u95f4\u7684\u5dee\u5f02<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\">2. \u5b83\u4e0d\u80fd\u5c55\u793a\u6570\u636e\u70b9\u4e4b\u95f4\u7684\u76f8\u5173\u6027\u6216\u805a\u7c7b\u60c5\u51b5\uff0c\u9700\u8981\u7ed3\u5408\u5176\u4ed6\u56fe\u5f62\u5982\u70ed\u56fe\u7b49<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\">3. \u5b83\u53ef\u80fd\u53d7\u5230\u6570\u636e\u6807\u51c6\u5316\u3001\u6279\u6b21\u6548\u5e94\u7b49\u56e0\u7d20\u7684\u5f71\u54cd\uff0c\u5bfc\u81f4\u6570\u636e\u5b58\u5728\u7cfb\u7edf\u504f\u5dee<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4efb\u4f55\u4e8b\u7269\u90fd\u4e0d\u4f1a\u662f\u5341\u5168\u5341\u7f8e\u7684\uff0c\u706b\u5c71\u56fe\u4e5f\u662f\u5982\u6b64\u3002\u4e0d\u8fc7\u6211\u4eec\u66f4\u5173\u6ce8\u5b83\u7684\u4f18\u70b9\u3002\u4e0b\u9762\u5c0f\u679c\u5e26\u5927\u5bb6\u4f7f\u7528ggplot2\u5305\u6765\u753b\u51fa\u7f8e\u89c2\u7684\u706b\u5c71\u56fe<\/span><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u9996\u5148\u662f\u6570\u636e\u5c55\u793a\uff0c\u524d\u5341\u884c\u7684\u6570\u636e\u5982\u4e0b\u56fe\u6240\u793a<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9716088328075709\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_JBOods6A9BqF15QOibGsNpicrBC0zw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYnLTDvLVDfyJD83JfNG9oHkKka1MJBOods6A9BqF15QOibGsNpicrBC0zw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"317\" style=\"\"><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e0b\u9762\u5c31\u662f\u4ee3\u7801\u90e8\u5206\u5566<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">rm( <span class=\"code-snippet__built_in\">list<\/span> = ls())<\/span><\/code><code><span class=\"code-snippet_outer\">gc()<\/span><\/code><code><span class=\"code-snippet_outer\"># \u8bbe\u7f6e\u5de5\u4f5c\u8def\u5f84<\/span><\/code><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">'\/media\/desk16\/iyun010'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(tidyverse)<\/span><\/code><code><span class=\"code-snippet_outer\"># \u8bfb\u53d6\u6570\u636e\u96c6<\/span><\/code><code><span class=\"code-snippet_outer\">data <- read.csv(<span class=\"code-snippet__string\">'data.csv'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"># \u5c06p\u503c\u8fdb\u884c-<span class=\"code-snippet__built_in\">log10<\/span>\u8f6c\u5316<\/span><\/code><code><span class=\"code-snippet_outer\">volc_plot <- ggplot(data, aes(LogFC, -<span class=\"code-snippet__built_in\">log10<\/span>(PValue))) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(size = <span class=\"code-snippet__number\">0.4<\/span>) + # \u8c03\u6574\u70b9\u7684\u5927\u5c0f<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab(expression(<span class=\"code-snippet__string\">\"log\"<\/span>[<span class=\"code-snippet__number\">2<\/span>]*<span class=\"code-snippet__string\">\" fold change\"<\/span>)) + # \u4fee\u9970\u6a2a\u5750\u6807\u7684\u9898\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\"> ylab(expression(<span class=\"code-snippet__string\">\"-log\"<\/span>[<span class=\"code-snippet__number\">10<\/span>]*<span class=\"code-snippet__string\">\" p-value\"<\/span>)) + # \u4fee\u9970\u7eb5\u5750\u6807\u7684\u9898\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_x_continuous(limits = c(<span class=\"code-snippet__number\">-15<\/span>, <span class=\"code-snippet__number\">15<\/span>)) + # \u8c03\u6574\u6a2a\u5750\u6807\u7684\u8303\u56f4<\/span><\/code><code><span class=\"code-snippet_outer\"> theme_minimal()<\/span><\/code><code><span class=\"code-snippet_outer\">data1 <- data %>%<\/span><\/code><code><span class=\"code-snippet_outer\"> mutate(expression = case_when(LogFC >= <span class=\"code-snippet__number\">1<\/span> & PValue < <span class=\"code-snippet__number\">0.05<\/span> ~ <span class=\"code-snippet__string\">\"Up-regulated\"<\/span>, # \u4e0a\u8c03<\/span><\/code><code><span class=\"code-snippet_outer\"> LogFC <= <span class=\"code-snippet__number\">-1<\/span> & PValue < <span class=\"code-snippet__number\">0.05<\/span> ~ <span class=\"code-snippet__string\">\"Down-regulated\"<\/span>, # \u4e0b\u8c03<\/span><\/code><code><span class=\"code-snippet_outer\"> TRUE ~ <span class=\"code-snippet__string\">\"Unchanged\"<\/span>)) # \u4e0d\u53d8<\/span><\/code><code><span class=\"code-snippet_outer\">volc_plot1 <- ggplot(data1, aes(LogFC, -<span class=\"code-snippet__built_in\">log10<\/span>(PValue))) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(size = <span class=\"code-snippet__number\">0.4<\/span>, aes(color = expression)) + # \u6839\u636eexpression\u6c34\u5e73\u8fdb\u884c\u7740\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab(expression(<span class=\"code-snippet__string\">\"log\"<\/span>[<span class=\"code-snippet__number\">2<\/span>]*<span class=\"code-snippet__string\">\" fold change\"<\/span>)) + # \u4fee\u9970x\u8f74\u9898\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\"> ylab(expression(<span class=\"code-snippet__string\">\"-log\"<\/span>[<span class=\"code-snippet__number\">10<\/span>]*<span class=\"code-snippet__string\">\" p-value\"<\/span>)) + # \u4fee\u9970y\u8f74\u9898\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_x_continuous(limits = c(<span class=\"code-snippet__number\">-15<\/span>, <span class=\"code-snippet__number\">15<\/span>)) +<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_color_manual(values = c(<span class=\"code-snippet__string\">\"steelblue\"<\/span>, <span class=\"code-snippet__string\">\"grey\"<\/span>, <span class=\"code-snippet__string\">\"red\"<\/span>))+ # \u6dfb\u52a0\u4e09\u79cd\u989c\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"> # <span class=\"code-snippet__function\">theme_minimal<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__title\">theme_bw<\/span><span class=\"code-snippet__params\">()<\/span> +<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__title\">theme<\/span><span class=\"code-snippet__params\">(panel.grid.major=element_blank(),panel.grid.minor=element_blank())<\/span>+<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__title\">theme<\/span><span class=\"code-snippet__params\">(axis.line = element_line(colour = <span class=\"code-snippet__string\">\"black\"<\/span>))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">ggsave<\/span><span class=\"code-snippet__params\">(<span class=\"code-snippet__string\">'volc.png'<\/span>, plot = last_plot(),width=<span class=\"code-snippet__number\">225<\/span>, height =<span class=\"code-snippet__number\">150<\/span>,units = <span class=\"code-snippet__string\">\"mm\"<\/span> ,dpi=<span class=\"code-snippet__number\">300<\/span>)<\/span><\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;letter-spacing: 0.034em;\">\u753b\u51fa\u7ed3\u679c\u5982\u4e0b\u56fe\u6240\u793a\uff0c\u662f\u4e0d\u662f\u5f88\u7f8e\u89c2\u5462<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6660649819494585\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_UDezZLMz0SdCeYDVicDH1zrzV2BWmA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYnVbb3bl9micia6NJC8QUpicaGamkcUDezZLMz0SdCeYDVicDH1zrzV2BWmA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4eca\u5929\u7684\u706b\u5c71\u56fe\u7ed8\u5236\u6559\u7a0b\u5c31\u5230\u8fd9\u91cc\u5566\uff0c\u611f\u5174\u8da3\u7684\u53ef\u4ee5\u627e\u5c0f\u679c\u8ba8\u8bba\u54e6\uff0c\u6211\u4eec\u4e0b\u6b21\u89c1~<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"578\" data-cropsely1=\"0\" data-cropsely2=\"322\" data-galleryid=\"\" data-ratio=\"0.7193195625759417\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_oRxs73HBHUoxCtAqBPQnq1AXricRvg-10.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxenwVZiaibuOTpj6MMDUj2LoPdS6tic40bbGQV7QyM6QOQY8EFoRxs73HBHUoxCtAqBPQnq1AXricRvg\/640?wx_fmt=png'\" data-type=\"jpeg\" data-w=\"823\" style=\"width: 578px;height: 416px;\"><\/p>\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 <\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br 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flex-start;flex: 0 0 auto;\">\n<section style=\"color: rgba(0, 0, 0, 0.5);text-align: justify;font-size: 14px;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\">\u706b\u5c71\u56fe\u76f8\u4fe1\u5927\u5bb6\u80af\u5b9a\u90fd\u4e0d\u964c\u751f\uff0c\u4f46\u662f\u600e\u4e48\u753b\u51fa\u7f8e\u89c2\u800c\u53c8\u5927\u65b9\u7684\u706b\u5c71\u56fe\u53ef\u8ba9\u5f88\u591a\u5c0f\u4f19\u4f34\u6293\u8033\u6320\u816e\u4e86\uff0c\u4e0b\u9762\u5c0f\u679c\u5316\u8eab\u8001\u53f8\u673a\u5e26\u5c0f\u4f19\u4f34\u4eec\u4e00\u540c\u5b66\u4e60\u753b\u76f4\u89c2\u7684\u706b\u5c71\u56fe\u5427<\/span><span style=\"letter-spacing: 0.034em;text-align: center;font-size: 16px;color: rgba(0, 0, 0, 0.9);\"><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u706b\u5c71\u56fe\u662f\u6563\u70b9\u56fe\u7684\u4e00\u79cd\uff0c\u5b83\u5c06\u7edf\u8ba1\u6d4b\u8bd5\u4e2d\u7684\u7edf\u8ba1\u663e\u8457\u6027\u91cf\u5ea6\uff08\u5982p value\uff09\u548c\u53d8\u5316\u5e45\u5ea6\u76f8\u7ed3\u5408\uff0c\u4ece\u800c\u80fd\u591f\u5e2e\u52a9\u5feb\u901f\u76f4\u89c2\u5730\u8bc6\u522b\u90a3\u4e9b\u53d8\u5316\u5e45\u5ea6\u8f83\u5927\u4e14\u5177\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\u7684\u6570\u636e\u70b9\uff08\u57fa\u56e0\u7b49\uff09\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u706b\u5c71\u56fe\u5e38\u5e94\u7528\u4e8e\u8f6c\u5f55\u7ec4\u7814\u7a76\uff0c\u4e5f\u80fd\u5e94\u7528\u4e8e\u57fa\u56e0\u7ec4\uff0c\u86cb\u767d\u8d28\u7ec4\uff0c\u4ee3\u8c22\u7ec4\u7b49\u7edf\u8ba1\u6570\u636e\u3002\u706b\u5c71\u56fe\u7684\u6a2a\u8f74\u4e00\u822c\u662f Log2 (fold change) \uff0c\u8868\u793a\u4e24\u7ec4\u6837\u54c1\u4e4b\u95f4\u7684\u5dee\u5f02\u500d\u6570\uff0c\u70b9\u8d8a\u504f\u79bb\u4e2d\u5fc3\uff0c\u8868\u793a\u5dee\u5f02\u500d\u6570\u8d8a\u5927\u3002\u706b\u5c71\u56fe\u7684\u7eb5\u8f74\u4e00\u822c\u662f -Log 10 (P-value) \uff0c\u8868\u793a\u5dee\u5f02\u662f\u5426\u5177\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\uff0c\u70b9\u8d8a\u9760\u56fe\u7684\u9876\u90e8\u8868\u793a\u5dee\u5f02\u8d8a\u663e\u8457\u3002\u706b\u5c71\u56fe\u7684\u70b9\u7684\u989c\u8272\u548c\u5927\u5c0f\u4e5f\u53ef\u4ee5\u8868\u793a\u66f4\u591a\u7684\u5c5e\u6027\uff0c\u5982\u57fa\u56e0\u7684\u4e0a\u8c03\u3001\u4e0b\u8c03\u3001\u65e0\u5dee\u5f02\u3001\u8868\u8fbe\u4e30\u5ea6\u7b49\u3002\u706b\u5c71\u56fe\u53ef\u4ee5\u5e2e\u52a9\u6211\u4eec\u5feb\u901f\u7b5b\u9009\u51fa\u5177\u6709\u751f\u7269\u5b66\u610f\u4e49\u7684\u5dee\u5f02\u57fa\u56e0\uff0c\u5e76\u5728\u56fe\u4e0a\u6807\u8bb0\u51fa\u6765\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4ecb\u7ecd\u5b8c\u4e86\u706b\u5c71\u56fe\uff0c\u4e3a\u4e86\u5c0f\u4f19\u4f34\u4eec\u66f4\u52a0\u5168\u9762\u7684\u8ba4\u8bc6\u706b\u5c71\u56fe\uff0c\u4e0b\u9762\u5c0f\u679c\u8bf4\u4e00\u4e0b\u5b83\u7684\u4f18\u7f3a\u70b9\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"font-size: 14px;\">\u706b\u5c71\u56fe\u7684\u4f18\u70b9\uff1a<\/span><\/strong><\/p>\n<\/section>\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"border-width: 1px;border-style: solid;border-color: black;padding: 3px;\">\n<section style=\"border-color: black;border-width: 3px;border-style: solid;padding: 10px;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\">1. \u5b83\u53ef\u4ee5\u5feb\u901f\u76f4\u89c2\u5730\u5c55\u793a\u51fa\u53d8\u5316\u5e45\u5ea6\u8f83\u5927\u4e14\u5177\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\u7684\u6570\u636e\u70b9\uff0c\u5982\u5dee\u5f02\u57fa\u56e0\u7b49<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\">2. \u5b83\u53ef\u4ee5\u5728\u56fe\u4e0a\u6807\u8bb0\u51fa\u5173\u6ce8\u7684\u57fa\u56e0\u7684\u540d\u5b57\uff0c\u65b9\u4fbf\u540e\u7eed\u7684\u5206\u6790\u548c\u9a8c\u8bc1<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\">3. \u5b83\u53ef\u4ee5\u7528\u70b9\u7684\u989c\u8272\u548c\u5927\u5c0f\u8868\u793a\u66f4\u591a\u7684\u5c5e\u6027\uff0c\u5982\u57fa\u56e0\u7684\u4e0a\u8c03\u3001\u4e0b\u8c03\u3001\u8868\u8fbe\u4e30\u5ea6\u7b49<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"font-size: 14px;\">\u706b\u5c71\u56fe\u7684\u7f3a\u70b9\uff1a<\/span><\/strong><\/p>\n<\/section>\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"border-width: 1px;border-style: solid;border-color: black;padding: 3px;\">\n<section style=\"border-color: black;border-width: 3px;border-style: solid;padding: 10px;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\">1. \u5b83\u53ea\u9002\u5408\u5c55\u793a\u4e24\u7ec4\u6837\u54c1\u4e4b\u95f4\u7684\u6bd4\u8f83\uff0c\u4e0d\u80fd\u5c55\u793a\u591a\u7ec4\u6837\u54c1\u4e4b\u95f4\u7684\u5dee\u5f02<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\">2. \u5b83\u4e0d\u80fd\u5c55\u793a\u6570\u636e\u70b9\u4e4b\u95f4\u7684\u76f8\u5173\u6027\u6216\u805a\u7c7b\u60c5\u51b5\uff0c\u9700\u8981\u7ed3\u5408\u5176\u4ed6\u56fe\u5f62\u5982\u70ed\u56fe\u7b49<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14px;\">3. \u5b83\u53ef\u80fd\u53d7\u5230\u6570\u636e\u6807\u51c6\u5316\u3001\u6279\u6b21\u6548\u5e94\u7b49\u56e0\u7d20\u7684\u5f71\u54cd\uff0c\u5bfc\u81f4\u6570\u636e\u5b58\u5728\u7cfb\u7edf\u504f\u5dee<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4efb\u4f55\u4e8b\u7269\u90fd\u4e0d\u4f1a\u662f\u5341\u5168\u5341\u7f8e\u7684\uff0c\u706b\u5c71\u56fe\u4e5f\u662f\u5982\u6b64\u3002\u4e0d\u8fc7\u6211\u4eec\u66f4\u5173\u6ce8\u5b83\u7684\u4f18\u70b9\u3002\u4e0b\u9762\u5c0f\u679c\u5e26\u5927\u5bb6\u4f7f\u7528ggplot2\u5305\u6765\u753b\u51fa\u7f8e\u89c2\u7684\u706b\u5c71\u56fe<\/span><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u9996\u5148\u662f\u6570\u636e\u5c55\u793a\uff0c\u524d\u5341\u884c\u7684\u6570\u636e\u5982\u4e0b\u56fe\u6240\u793a<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9716088328075709\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_JBOods6A9BqF15QOibGsNpicrBC0zw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYnLTDvLVDfyJD83JfNG9oHkKka1MJBOods6A9BqF15QOibGsNpicrBC0zw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"317\" style=\"\"><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e0b\u9762\u5c31\u662f\u4ee3\u7801\u90e8\u5206\u5566<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">rm( <span class=\"code-snippet__built_in\">list<\/span> = ls())<\/span><\/code><code><span class=\"code-snippet_outer\">gc()<\/span><\/code><code><span class=\"code-snippet_outer\"># \u8bbe\u7f6e\u5de5\u4f5c\u8def\u5f84<\/span><\/code><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">'\/media\/desk16\/iyun010'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(tidyverse)<\/span><\/code><code><span class=\"code-snippet_outer\"># \u8bfb\u53d6\u6570\u636e\u96c6<\/span><\/code><code><span class=\"code-snippet_outer\">data <- read.csv(<span class=\"code-snippet__string\">'data.csv'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"># \u5c06p\u503c\u8fdb\u884c-<span class=\"code-snippet__built_in\">log10<\/span>\u8f6c\u5316<\/span><\/code><code><span class=\"code-snippet_outer\">volc_plot <- ggplot(data, aes(LogFC, -<span class=\"code-snippet__built_in\">log10<\/span>(PValue))) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(size = <span class=\"code-snippet__number\">0.4<\/span>) + # \u8c03\u6574\u70b9\u7684\u5927\u5c0f<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab(expression(<span class=\"code-snippet__string\">\"log\"<\/span>[<span class=\"code-snippet__number\">2<\/span>]*<span class=\"code-snippet__string\">\" fold change\"<\/span>)) + # \u4fee\u9970\u6a2a\u5750\u6807\u7684\u9898\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\"> ylab(expression(<span class=\"code-snippet__string\">\"-log\"<\/span>[<span class=\"code-snippet__number\">10<\/span>]*<span class=\"code-snippet__string\">\" p-value\"<\/span>)) + # \u4fee\u9970\u7eb5\u5750\u6807\u7684\u9898\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_x_continuous(limits = c(<span class=\"code-snippet__number\">-15<\/span>, <span class=\"code-snippet__number\">15<\/span>)) + # \u8c03\u6574\u6a2a\u5750\u6807\u7684\u8303\u56f4<\/span><\/code><code><span class=\"code-snippet_outer\"> theme_minimal()<\/span><\/code><code><span class=\"code-snippet_outer\">data1 <- data %>%<\/span><\/code><code><span class=\"code-snippet_outer\"> mutate(expression = case_when(LogFC >= <span class=\"code-snippet__number\">1<\/span> & PValue < <span class=\"code-snippet__number\">0.05<\/span> ~ <span class=\"code-snippet__string\">\"Up-regulated\"<\/span>, # \u4e0a\u8c03<\/span><\/code><code><span class=\"code-snippet_outer\"> LogFC <= <span class=\"code-snippet__number\">-1<\/span> & PValue < <span class=\"code-snippet__number\">0.05<\/span> ~ <span class=\"code-snippet__string\">\"Down-regulated\"<\/span>, # \u4e0b\u8c03<\/span><\/code><code><span class=\"code-snippet_outer\"> TRUE ~ <span class=\"code-snippet__string\">\"Unchanged\"<\/span>)) # \u4e0d\u53d8<\/span><\/code><code><span class=\"code-snippet_outer\">volc_plot1 <- ggplot(data1, aes(LogFC, -<span class=\"code-snippet__built_in\">log10<\/span>(PValue))) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(size = <span class=\"code-snippet__number\">0.4<\/span>, aes(color = expression)) + # \u6839\u636eexpression\u6c34\u5e73\u8fdb\u884c\u7740\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab(expression(<span class=\"code-snippet__string\">\"log\"<\/span>[<span class=\"code-snippet__number\">2<\/span>]*<span class=\"code-snippet__string\">\" fold change\"<\/span>)) + # \u4fee\u9970x\u8f74\u9898\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\"> ylab(expression(<span class=\"code-snippet__string\">\"-log\"<\/span>[<span class=\"code-snippet__number\">10<\/span>]*<span class=\"code-snippet__string\">\" p-value\"<\/span>)) + # \u4fee\u9970y\u8f74\u9898\u76ee<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_x_continuous(limits = c(<span class=\"code-snippet__number\">-15<\/span>, <span class=\"code-snippet__number\">15<\/span>)) +<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_color_manual(values = c(<span class=\"code-snippet__string\">\"steelblue\"<\/span>, <span class=\"code-snippet__string\">\"grey\"<\/span>, <span class=\"code-snippet__string\">\"red\"<\/span>))+ # \u6dfb\u52a0\u4e09\u79cd\u989c\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"> # <span class=\"code-snippet__function\">theme_minimal<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__title\">theme_bw<\/span><span class=\"code-snippet__params\">()<\/span> +<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__title\">theme<\/span><span class=\"code-snippet__params\">(panel.grid.major=element_blank(),panel.grid.minor=element_blank())<\/span>+<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__title\">theme<\/span><span class=\"code-snippet__params\">(axis.line = element_line(colour = <span class=\"code-snippet__string\">\"black\"<\/span>))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">ggsave<\/span><span class=\"code-snippet__params\">(<span class=\"code-snippet__string\">'volc.png'<\/span>, plot = last_plot(),width=<span class=\"code-snippet__number\">225<\/span>, height =<span class=\"code-snippet__number\">150<\/span>,units = <span class=\"code-snippet__string\">\"mm\"<\/span> ,dpi=<span class=\"code-snippet__number\">300<\/span>)<\/span><\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;letter-spacing: 0.034em;\">\u753b\u51fa\u7ed3\u679c\u5982\u4e0b\u56fe\u6240\u793a\uff0c\u662f\u4e0d\u662f\u5f88\u7f8e\u89c2\u5462<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6660649819494585\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_UDezZLMz0SdCeYDVicDH1zrzV2BWmA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYnVbb3bl9micia6NJC8QUpicaGamkcUDezZLMz0SdCeYDVicDH1zrzV2BWmA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4eca\u5929\u7684\u706b\u5c71\u56fe\u7ed8\u5236\u6559\u7a0b\u5c31\u5230\u8fd9\u91cc\u5566\uff0c\u611f\u5174\u8da3\u7684\u53ef\u4ee5\u627e\u5c0f\u679c\u8ba8\u8bba\u54e6\uff0c\u6211\u4eec\u4e0b\u6b21\u89c1~<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"578\" data-cropsely1=\"0\" data-cropsely2=\"322\" data-galleryid=\"\" data-ratio=\"0.7193195625759417\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_oRxs73HBHUoxCtAqBPQnq1AXricRvg-10.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxenwVZiaibuOTpj6MMDUj2LoPdS6tic40bbGQV7QyM6QOQY8EFoRxs73HBHUoxCtAqBPQnq1AXricRvg\/640?wx_fmt=png'\" data-type=\"jpeg\" data-w=\"823\" style=\"width: 578px;height: 416px;\"><\/p>\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 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