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255, 255, 0) rgb(252, 210, 125) rgb(252, 210, 125);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"transform: rotateZ(348deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: -37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>\u51c6\u5907\u9700\u8981\u7684R\u5305<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">library(Seurat)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\">library(patchwork)<\/span><\/code><code><span class=\"code-snippet_outer\">library(magrittr)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: center;\">\n<section style=\"transform: rotateZ(328deg) scale(0.6);transform-origin: center center;margin: -20px -19px;max-width: unset !important;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;margin: 14px 0% 11px;transform: translate3d(5px, 0px, 0px);display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 51px;height: 47px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgba(255, 255, 255, 0) rgb(252, 210, 125) rgb(252, 210, 125);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"transform: rotateZ(348deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: -37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>\u6570\u636e\u51c6\u5907<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u6570\u636e\u4e3a\u6807\u51c6\u768410x\u5355\u7ec6\u80de\u6570\u636e\uff0c\u5c06\u6570\u636e\u4fdd\u5b58\u5728\u4e00\u4e2a\u6587\u4ef6\u5939\u4e2d\uff0c\u5305\u62ecbarcodes.tsv\uff0cgenes.tsv\u548cmatrix.mtx\u4e09\u4e2a\u6587\u4ef6\u3002<br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.10277777777777777\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_45QW4qyTfG1GGaq0scSQUeK2B8RVIA.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/oCSOtP61aEXahxt3mWw4QwXurdfBUNx19jIOMSsBU5OHxCuxyqTa3pmX45QW4qyTfG1GGaq0scSQUeK2B8RVIA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\"><\/section>\n<\/section>\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: center;\">\n<section style=\"transform: rotateZ(328deg) scale(0.6);transform-origin: center center;margin: -20px -19px;max-width: unset !important;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;margin: 14px 0% 11px;transform: translate3d(5px, 0px, 0px);display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 51px;height: 47px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgba(255, 255, 255, 0) rgb(252, 210, 125) rgb(252, 210, 125);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"transform: rotateZ(348deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: -37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>scRNA\u5e38\u89c4\u5206\u6790\u6d41\u7a0b<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d610x\u5355\u7ec6\u80de\u6570\u636e\uff0c\u9700\u8981\u63d0\u4f9b\u6570\u636e\u6240\u5728\u6587\u4ef6\u5939\u8def\u5f84<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc.data <- Read10X(data.dir = hg19)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5efa\u7acb\u5355\u7ec6\u80de\u5bf9\u8c61<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- CreateSeuratObject(counts = pbmc.data, project = <span class=\"code-snippet__string\">\"pbmc3k\"<\/span>, min.cells = <span class=\"code-snippet__number\">3<\/span>, min.features = <span class=\"code-snippet__number\">200<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8ba1\u7b97\u7ebf\u7c92\u4f53\u57fa\u56e0\u6bd4\u4f8b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc[[<span class=\"code-snippet__string\">\"percent.mt\"<\/span>]] <- PercentageFeatureSet(pbmc, pattern = <span class=\"code-snippet__string\">\"^MT-\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u901a\u8fc7\u63a7\u5236\u57fa\u56e0\u6570\u548c\u7ebf\u7c92\u4f53\u57fa\u56e0\u6bd4\u4f8b\u8fc7\u6ee4\u6389\u4f4e\u8d28\u91cf\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- subset(pbmc, subset = nFeature_RNA > <span class=\"code-snippet__number\">200<\/span> & nFeature_RNA < <span class=\"code-snippet__number\">2500<\/span> & percent.mt < <span class=\"code-snippet__number\">5<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8fdb\u884cnormalize\u5e76\u8ba1\u7b97\u9ad8\u53d8\u5f02\u57fa\u56e0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- NormalizeData(pbmc)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- FindVariableFeatures(pbmc, selection.method = <span class=\"code-snippet__string\">\"vst\"<\/span>, nfeatures = <span class=\"code-snippet__number\">2000<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Scale\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">all.genes <- rownames(pbmc)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- ScaleData(pbmc, features = all.genes)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># PCA<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- RunPCA(pbmc, features = VariableFeatures(object = pbmc))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u9009\u62e9\u524d10\u4e2apc\u57fa\u56e0\u964d\u7ef4\uff0c\u91c7\u7528UMAP\u65b9\u5f0f<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- FindNeighbors(pbmc, dims = <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- FindClusters(pbmc, resolution = <span class=\"code-snippet__number\">0.5<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- RunUMAP(pbmc, dims = <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u624b\u52a8\u6dfb\u52a0\u6ce8\u91ca<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">new.cluster.ids <- <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Naive CD4 T\"<\/span>, <span class=\"code-snippet__string\">\"Memory CD4 T\"<\/span>, <span class=\"code-snippet__string\">\"CD14+ Mono\"<\/span>, <span class=\"code-snippet__string\">\"B\"<\/span>, <span class=\"code-snippet__string\">\"CD8 T\"<\/span>, <span class=\"code-snippet__string\">\"FCGR3A+ Mono\"<\/span>, <span class=\"code-snippet__string\">\"NK\"<\/span>, <span class=\"code-snippet__string\">\"DC\"<\/span>, <span class=\"code-snippet__string\">\"Platelet\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">names<\/span>(new.cluster.ids) <- levels(pbmc)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- RenameIdents(pbmc, new.cluster.ids)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc[[<span class=\"code-snippet__string\">'cell_type'<\/span>]] <- pbmc@active.ident<\/span><\/code><code><span class=\"code-snippet_outer\">levels(pbmc)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: center;\">\n<section style=\"transform: rotateZ(328deg) scale(0.6);transform-origin: center center;margin: -20px -19px;max-width: unset !important;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;margin: 14px 0% 11px;transform: translate3d(5px, 0px, 0px);display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 51px;height: 47px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgba(255, 255, 255, 0) rgb(252, 210, 125) rgb(252, 210, 125);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"transform: rotateZ(348deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: -37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>FeaturePlot\u7ed8\u5236UMAP\u56fe\u4e0a\u5171\u8868\u8fbe\u57fa\u56e0\u5c55\u793a\uff0cblend\u8bbe\u7f6e\u4e3aTRUE<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">FeaturePlot(pbmc, features = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"CD3G\"<\/span>, <span class=\"code-snippet__string\">\"CD247\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">blend = <span class=\"code-snippet__literal\">TRUE<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">cols = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"gray80\"<\/span>,<span class=\"code-snippet__string\">\"red\"<\/span>, <span class=\"code-snippet__string\">\"green\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">pt.size = <span class=\"code-snippet__number\">0.5<\/span>, raster = <span class=\"code-snippet__built_in\">F<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">theme(aspect.ratio = <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(<span class=\"code-snippet__string\">\"UMAP_co-expression_matrix.pdf\"<\/span>, width = <span class=\"code-snippet__number\">12<\/span>, height = <span class=\"code-snippet__number\">4<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: center;\">\n<section style=\"transform: rotateZ(328deg) scale(0.6);transform-origin: center center;margin: -20px -19px;max-width: unset !important;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;margin: 14px 0% 11px;transform: translate3d(5px, 0px, 0px);display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 51px;height: 47px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgba(255, 255, 255, 0) rgb(252, 210, 125) rgb(252, 210, 125);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"transform: rotateZ(348deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: -37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>\u81ea\u5b9a\u4e49\u51fd\u6570\u8ba1\u7b97\u5171\u8868\u8fbe\u57fa\u56e0\u76f8\u5173\u6027\u5e76\u53ef\u89c6\u5316<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#object\u5bf9seurat\u5bf9\u8c61\uff0cgene1\u548cgene2\u4e3a\u4e24\u4e2a\u5171\u8868\u8fbe\u57fa\u56e0\u540d\uff0ccor.method\u4e3a\u8ba1\u7b97\u5404\u76f8\u5173\u6027\u65b9\u6cd5\uff0c\u53ef\u9009spearman\u6216pearson<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u4e3a\u4e86\u9632\u6b62\u6240\u6709\u6570\u636e\u805a\u96c6\u5728\u4e00\u8d77\uff0c\u8fd9\u662fjitter.num\u4f7f\u6570\u636e\u8fdb\u884c\u8f7b\u5fae\u6296\u52a8\uff0c\u8bbe\u7f6e\u4e3a0\u5219\u4e3a\u539f\u59cb\u6570\u636e\u7ed8\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># pos\u82e5\u4e3aTRUE\uff0c\u600e\u53ea\u4f7f\u7528\u4e24\u4e2a\u57fa\u56e0\u90fd\u8868\u8fbe\u7684\u7ec6\u80de\u8ba1\u7b97\u76f8\u5173\u6027\uff0c\u53ef\u907f\u514d\u597d\u591a\u7ec6\u80de\u4e2d\u5bf9\u5176\u4e2d\u4e00\u4e2a\u7ec6\u80de\u8868\u8fbe\u91cf\u4e3a0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">getScatterplot <- <span class=\"code-snippet__keyword\">function<\/span>(object, gene1, gene2, cor.method = <span class=\"code-snippet__string\">\"spearman\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">jitter.num = <span class=\"code-snippet__number\">0.15<\/span>, pos = <span class=\"code-snippet__literal\">TRUE<\/span>){<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span> (!gene1 %in% rownames(object)) {<\/span><\/code><code><span class=\"code-snippet_outer\">print(<span class=\"code-snippet__string\">\"gene1 was not found\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span> (!gene2 %in% rownames(object)) {<\/span><\/code><code><span class=\"code-snippet_outer\">print(<span class=\"code-snippet__string\">\"gene2 was not found\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">}<span class=\"code-snippet__keyword\">else<\/span>{<\/span><\/code><code><span class=\"code-snippet_outer\">exp.mat <- GetAssayData(object = object, assay = <span class=\"code-snippet__string\">\"RNA\"<\/span>) %>% .[<span class=\"code-snippet__built_in\">c<\/span>(gene1,gene2),] %>%<\/span><\/code><code><span class=\"code-snippet_outer\">as.matrix() %>% t() %>% as.data.frame()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span> (pos) {<\/span><\/code><code><span class=\"code-snippet_outer\">exp.mat <- exp.mat[which(exp.mat[,<span class=\"code-snippet__number\">1<\/span>] > <span class=\"code-snippet__number\">0<\/span> & exp.mat[,<span class=\"code-snippet__number\">2<\/span>] > <span class=\"code-snippet__number\">0<\/span>),]<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">plots <- ggplot(data=exp.mat, mapping = aes_string(x = gene1, y = gene2)) +<\/span><\/code><code><span class=\"code-snippet_outer\">geom_smooth(method = <span class=\"code-snippet__string\">'lm'<\/span>, se = <span class=\"code-snippet__built_in\">T<\/span>, color=<span class=\"code-snippet__string\">'red'<\/span>, size=<span class=\"code-snippet__number\">1<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">stat_cor(method = cor.method)+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_jitter(width = jitter.num, height = jitter.num, color = <span class=\"code-snippet__string\">\"black\"<\/span>, size = <span class=\"code-snippet__number\">1<\/span>, alpha=<span class=\"code-snippet__number\">1<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw()+<\/span><\/code><code><span class=\"code-snippet_outer\">theme(panel.grid=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.text=element_text(colour= <span class=\"code-snippet__string\">'black'<\/span>,size=<span class=\"code-snippet__number\">10<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text= element_text(colour= <span class=\"code-snippet__string\">'black'<\/span>,size=<span class=\"code-snippet__number\">10<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.line= element_line(colour= <span class=\"code-snippet__string\">'black'<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.border = element_rect(size = <span class=\"code-snippet__number\">1<\/span>, linetype = <span class=\"code-snippet__string\">\"solid\"<\/span>, colour = <span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.background=element_rect(fill=<span class=\"code-snippet__string\">\"white\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">return<\/span>(plots)<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8ba1\u7b97\u5171\u8868\u8fbe\u57fa\u56e0\u76f8\u5173\u6027\u5e76\u53ef\u89c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">getScatterplot(pbmc3k, gene1 = <span class=\"code-snippet__string\">\"CD3G\"<\/span>, gene2 = <span class=\"code-snippet__string\">\"CD247\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">jitter.num = <span class=\"code-snippet__number\">0.15<\/span>, pos = <span class=\"code-snippet__literal\">TRUE<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">theme(aspect.ratio = <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(<span class=\"code-snippet__string\">\"correlation_dotplot.pdf\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: center;\">\n<section style=\"transform: rotateZ(328deg) scale(0.6);transform-origin: center center;margin: -20px -19px;max-width: unset !important;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;margin: 14px 0% 11px;transform: translate3d(5px, 0px, 0px);display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 51px;height: 47px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgba(255, 255, 255, 0) rgb(252, 210, 125) rgb(252, 210, 125);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"transform: rotateZ(348deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: -37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>\u7ed3\u679c\u6587\u4ef6<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>1.UMAP_co-expression_matrix.pdf<\/strong><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u8be5\u7ed3\u679c\u56fe\u7247\u4e3aCD3G\u548cCD247\u8fd9\u4e24\u4e2aT\u7ec6\u80demarker\u57fa\u56e0\u8fdb\u884c\u57fa\u56e0\u5171\u8868\u8fbe\u53ef\u89c6\u5316\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.34444444444444444\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_FxlGbibxQsTnO7icwxzXJuW2B0zicw.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/oCSOtP61aEXahxt3mWw4QwXurdfBUNx155wa3ZDZoWAJh9DytqN1tBMfwa3FxlGbibxQsTnO7icwxzXJuW2B0zicw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>2.correlation_dotplot.pdf<\/strong><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u8be5\u7ed3\u679c\u56fe\u7247\u4e3a\u5171\u8868\u8fbe\u57fa\u56e0\u7684\u76f8\u5173\u6027\u6563\u70b9\u56fe\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9735376044568245\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_BF6Dic01R1N5sgCkxPMiaxad0MzI5g.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/oCSOtP61aEXahxt3mWw4QwXurdfBUNx1eGtv9yE6IbiaWAspzf8C9eXCDQNBF6Dic01R1N5sgCkxPMiaxad0MzI5g\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"718\" style=\"vertical-align: middle;width: 100%;\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u4eca\u5929\u5c0f\u5e08\u59b9\u5bf9\u5355\u7ec6\u80de\u57fa\u56e0\u5171\u8868\u8fbe\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\u5566\uff0c\u8fdb\u884c\u8be5\u5206\u6790\u9700\u8981\u719f\u7ec3\u638c\u63e1\u5355\u7ec6\u80de\u5206\u6790\u6d41\u7a0b\uff0c\u5355\u7ec6\u80de\u76f8\u5173\u5206\u6790\u5185\u5bb9\u53ef\u4ee5\u5c1d\u8bd5\u4f7f\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u4e91\u5e73\u53f0\u751f\u7269\u4fe1\u606f\u5206\u6790\u5c0f\u5de5\u5177\uff0c\u96f6\u4ee3\u7801\u5b8c\u6210\u5206\u6790\uff0c<span style=\"font-size: 16px;letter-spacing: 0.544px;\">\u5355\u7ec6\u80de\u5206\u6790\u76f8\u5173\u5c0f\u5de5\u5177\uff0c\u6b22\u8fce\u5c0f\u4f19\u4f34\u6765\u5c1d\u8bd5\u54df\uff01<\/span><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>\u4e91\u5e73\u53f0\u7f51\u5740\uff1a<\/strong><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">http:\/\/www.biocloudservice.com\/home.html<\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>\u5355\u7ec6\u80de\u5206\u6790\uff1a<\/strong><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">http:\/\/www.biocloudservice.com\/366\/366.php<\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>\u5355\u7ec6\u80de\u6570\u636e\u7ed8\u5236\u5c0f\u63d0\u7434\u56fe\uff1a<\/strong><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><span style=\"letter-spacing: 0.034em;\">http:\/\/www.biocloudservice.com\/788\/788.php<\/span><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>\u7ed8\u5236\u5355\u7ec6\u80detSNE\u56fe\uff1a<\/strong><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">http:\/\/www.biocloudservice.com\/229\/229.php<span style=\"letter-spacing: 0.034em;\"><\/span><\/p>\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;height: auto;align-self: center;margin-right: 10px;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(249, 172, 172);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);padding-right: 8px;padding-left: 8px;margin-right: 10px;\">\n<section style=\"text-align: justify;color: rgb(249, 172, 172);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">E<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);padding-right: 8px;padding-left: 8px;margin-right: 10px;\">\n<section style=\"text-align: justify;color: rgb(249, 172, 172);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">N<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);padding-right: 8px;padding-left: 8px;margin-right: 10px;\">\n<section style=\"text-align: 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powered-by=\"xiumi.us\">\u53ee\u549a\uff01\u4eca\u5929\u53c8\u5230\u5c0f\u5e08\u59b9\u5206\u4eab\u7684\u65f6\u95f4\u5566\uff01\u4eca\u5929\u5c0f\u5e08\u59b9\u53c8\u7ed9\u5927\u5bb6\u5e26\u6765\u4e86\u4e00\u4e2a\u65b0\u7684\u5206\u6790\u601d\u8def\uff0c\u662f\u4ec0\u4e48\u601d\u8def\u5462\uff1f\u5c55\u793a\u5355\u7ec6\u80de\u57fa\u56e0\u5171\u8868\u8fbe\uff0c\u4ee5\u53ca\u8fdb\u884c\u5171\u8868\u8fbe\u57fa\u56e0\u76f8\u5173\u6027\u5206\u6790\uff0c\u5c0f\u4f19\u4f34\u662f\u4e0d\u662f\u89c9\u5f97\u6709\u70b9\u610f\u601d\u5440\uff01\u90a3\u8fd8\u7b49\u7740\u5e72\u561b\uff01\u8d76\u7d27\u8ddf\u7740\u5c0f\u5e08\u59b9\u5f00\u542f\u4eca\u5929\u7684\u5b66\u4e60\u4e4b\u65c5\u5427\uff01<br \/><\/p>\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: 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-37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>\u5982\u4f55\u8fdb\u884c\u5355\u7ec6\u80de\u57fa\u56e0\u5171\u8868\u8fbe\u5206\u6790\uff1f<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u5355\u7ec6\u80de\u57fa\u56e0\u5171\u8868\u8fbe\u5206\u6790\uff1f\u5c0f\u5e08\u59b9\u4ee5\u524d\u597d\u50cf\u6ca1\u6709\u5728\u5355\u7ec6\u80de\u6570\u636e\u4e2d\u542c\u5230\u8fc7\u8be5\u5206\u6790\uff0c\u6709\u53ef\u80fd\u662f\u5c0f\u5e08\u59b9\u5b66\u827a\u4e0d\u7cbe\u5965\uff01\u54c8\u54c8\u54c8\u54c8\u54c8\uff01\u6211\u76f8\u4fe1\u8ddf\u7740\u5c0f\u5e08\u59b9\u4e00\u8d77\u5b66\u4e60\u4e4b\u540e\uff0c\u5c0f\u4f19\u4f34\u4eec\u5c31\u4f1a\u89c9\u5f97\u5f88\u7b80\u5355\u5566\uff01\u63a5\u4e0b\u6765\u5c31\u8ba9\u5c0f\u5e08\u59b9\u4e3a\u5927\u5bb6\u8bb2\u89e3\u4e00\u4e0b\u8be5\u5982\u4f55\u5206\u6790\uff1f\u9996\u5148\u9700\u8981\u5b8c\u6210\u5355\u7ec6\u80de\u6570\u636e\u5e38\u89c4\u5206\u6790\uff0c\u7136\u540e\u76f4\u63a5\u5229\u7528FeaturePlot\u51fd\u6570\u6765\u7ed8\u5236UMAP\u56fe\u4e0a\u5171\u8868\u8fbe\u57fa\u56e0\u53ef\u89c6\u5316\uff0c\u53ef\u4ee5\u9009\u62e9\u81ea\u5df1\u611f\u5174\u8da3\u7684\u4e24\u4e2a\u57fa\u56e0\u6765\u5c55\u793a\uff0c\u5f88\u65b9\u4fbf\uff0c\u5176\u5b9e\uff0c\u6700\u91cd\u8981\u7684\u662f\u5c0f\u4f19\u4f34\u8981\u638c\u63e1\u5355\u7ec6\u80de\u5206\u6790\u6d41\u7a0b\uff0c\u8fd9\u662f\u57fa\u7840\u5965\uff01\u8fdb\u884c\u57fa\u56e0\u5171\u8868\u8fbe\u53ef\u89c6\u5316\u5f88\u7b80\u5355\uff0c\u6b63\u5982\u5c0f\u5e08\u59b9\u6240\u8bf4\uff0c\u5982\u679c\u89c9\u5f97\u6709\u70b9\u6000\u7591\uff0c\u90a3\u5c31\u8ddf\u7740\u5c0f\u5e08\u59b9\u5f00\u59cb\u4eca\u5929\u7684\u5b9e\u64cd\u5427\uff01<\/p>\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: center;\">\n<section style=\"transform: rotateZ(328deg) scale(0.6);transform-origin: center center;margin: -20px -19px;max-width: unset !important;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;margin: 14px 0% 11px;transform: translate3d(5px, 0px, 0px);display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 51px;height: 47px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgba(255, 255, 255, 0) rgb(252, 210, 125) rgb(252, 210, 125);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"transform: rotateZ(348deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: -37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>\u51c6\u5907\u9700\u8981\u7684R\u5305<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">library(Seurat)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\">library(patchwork)<\/span><\/code><code><span class=\"code-snippet_outer\">library(magrittr)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: center;\">\n<section style=\"transform: rotateZ(328deg) scale(0.6);transform-origin: center center;margin: -20px -19px;max-width: unset !important;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;margin: 14px 0% 11px;transform: translate3d(5px, 0px, 0px);display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 51px;height: 47px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgba(255, 255, 255, 0) rgb(252, 210, 125) rgb(252, 210, 125);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"transform: rotateZ(348deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: -37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>\u6570\u636e\u51c6\u5907<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u6570\u636e\u4e3a\u6807\u51c6\u768410x\u5355\u7ec6\u80de\u6570\u636e\uff0c\u5c06\u6570\u636e\u4fdd\u5b58\u5728\u4e00\u4e2a\u6587\u4ef6\u5939\u4e2d\uff0c\u5305\u62ecbarcodes.tsv\uff0cgenes.tsv\u548cmatrix.mtx\u4e09\u4e2a\u6587\u4ef6\u3002<br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.10277777777777777\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_45QW4qyTfG1GGaq0scSQUeK2B8RVIA.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/oCSOtP61aEXahxt3mWw4QwXurdfBUNx19jIOMSsBU5OHxCuxyqTa3pmX45QW4qyTfG1GGaq0scSQUeK2B8RVIA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\"><\/section>\n<\/section>\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: center;\">\n<section style=\"transform: rotateZ(328deg) scale(0.6);transform-origin: center center;margin: -20px -19px;max-width: unset !important;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;margin: 14px 0% 11px;transform: translate3d(5px, 0px, 0px);display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 51px;height: 47px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgba(255, 255, 255, 0) rgb(252, 210, 125) rgb(252, 210, 125);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"transform: rotateZ(348deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: -37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>scRNA\u5e38\u89c4\u5206\u6790\u6d41\u7a0b<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d610x\u5355\u7ec6\u80de\u6570\u636e\uff0c\u9700\u8981\u63d0\u4f9b\u6570\u636e\u6240\u5728\u6587\u4ef6\u5939\u8def\u5f84<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc.data <- Read10X(data.dir = hg19)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5efa\u7acb\u5355\u7ec6\u80de\u5bf9\u8c61<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- CreateSeuratObject(counts = pbmc.data, project = <span class=\"code-snippet__string\">\"pbmc3k\"<\/span>, min.cells = <span class=\"code-snippet__number\">3<\/span>, min.features = <span class=\"code-snippet__number\">200<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8ba1\u7b97\u7ebf\u7c92\u4f53\u57fa\u56e0\u6bd4\u4f8b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc[[<span class=\"code-snippet__string\">\"percent.mt\"<\/span>]] <- PercentageFeatureSet(pbmc, pattern = <span class=\"code-snippet__string\">\"^MT-\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u901a\u8fc7\u63a7\u5236\u57fa\u56e0\u6570\u548c\u7ebf\u7c92\u4f53\u57fa\u56e0\u6bd4\u4f8b\u8fc7\u6ee4\u6389\u4f4e\u8d28\u91cf\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- subset(pbmc, subset = nFeature_RNA > <span class=\"code-snippet__number\">200<\/span> & nFeature_RNA < <span class=\"code-snippet__number\">2500<\/span> & percent.mt < <span class=\"code-snippet__number\">5<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8fdb\u884cnormalize\u5e76\u8ba1\u7b97\u9ad8\u53d8\u5f02\u57fa\u56e0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- NormalizeData(pbmc)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- FindVariableFeatures(pbmc, selection.method = <span class=\"code-snippet__string\">\"vst\"<\/span>, nfeatures = <span class=\"code-snippet__number\">2000<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Scale\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">all.genes <- rownames(pbmc)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- ScaleData(pbmc, features = all.genes)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># PCA<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- RunPCA(pbmc, features = VariableFeatures(object = pbmc))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u9009\u62e9\u524d10\u4e2apc\u57fa\u56e0\u964d\u7ef4\uff0c\u91c7\u7528UMAP\u65b9\u5f0f<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- FindNeighbors(pbmc, dims = <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- FindClusters(pbmc, resolution = <span class=\"code-snippet__number\">0.5<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- RunUMAP(pbmc, dims = <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u624b\u52a8\u6dfb\u52a0\u6ce8\u91ca<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">new.cluster.ids <- <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Naive CD4 T\"<\/span>, <span class=\"code-snippet__string\">\"Memory CD4 T\"<\/span>, <span class=\"code-snippet__string\">\"CD14+ Mono\"<\/span>, <span class=\"code-snippet__string\">\"B\"<\/span>, <span class=\"code-snippet__string\">\"CD8 T\"<\/span>, <span class=\"code-snippet__string\">\"FCGR3A+ Mono\"<\/span>, <span class=\"code-snippet__string\">\"NK\"<\/span>, <span class=\"code-snippet__string\">\"DC\"<\/span>, <span class=\"code-snippet__string\">\"Platelet\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">names<\/span>(new.cluster.ids) <- levels(pbmc)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- RenameIdents(pbmc, new.cluster.ids)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc[[<span class=\"code-snippet__string\">'cell_type'<\/span>]] <- pbmc@active.ident<\/span><\/code><code><span class=\"code-snippet_outer\">levels(pbmc)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: center;\">\n<section style=\"transform: rotateZ(328deg) scale(0.6);transform-origin: center center;margin: -20px -19px;max-width: unset !important;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;margin: 14px 0% 11px;transform: translate3d(5px, 0px, 0px);display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 51px;height: 47px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgba(255, 255, 255, 0) rgb(252, 210, 125) rgb(252, 210, 125);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"transform: rotateZ(348deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: -37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>FeaturePlot\u7ed8\u5236UMAP\u56fe\u4e0a\u5171\u8868\u8fbe\u57fa\u56e0\u5c55\u793a\uff0cblend\u8bbe\u7f6e\u4e3aTRUE<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">FeaturePlot(pbmc, features = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"CD3G\"<\/span>, <span class=\"code-snippet__string\">\"CD247\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">blend = <span class=\"code-snippet__literal\">TRUE<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">cols = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"gray80\"<\/span>,<span class=\"code-snippet__string\">\"red\"<\/span>, <span class=\"code-snippet__string\">\"green\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">pt.size = <span class=\"code-snippet__number\">0.5<\/span>, raster = <span class=\"code-snippet__built_in\">F<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">theme(aspect.ratio = <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(<span class=\"code-snippet__string\">\"UMAP_co-expression_matrix.pdf\"<\/span>, width = <span class=\"code-snippet__number\">12<\/span>, height = <span class=\"code-snippet__number\">4<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: center;\">\n<section style=\"transform: rotateZ(328deg) scale(0.6);transform-origin: center center;margin: -20px -19px;max-width: unset !important;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;margin: 14px 0% 11px;transform: translate3d(5px, 0px, 0px);display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 51px;height: 47px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgba(255, 255, 255, 0) rgb(252, 210, 125) rgb(252, 210, 125);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"transform: rotateZ(348deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: -37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>\u81ea\u5b9a\u4e49\u51fd\u6570\u8ba1\u7b97\u5171\u8868\u8fbe\u57fa\u56e0\u76f8\u5173\u6027\u5e76\u53ef\u89c6\u5316<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#object\u5bf9seurat\u5bf9\u8c61\uff0cgene1\u548cgene2\u4e3a\u4e24\u4e2a\u5171\u8868\u8fbe\u57fa\u56e0\u540d\uff0ccor.method\u4e3a\u8ba1\u7b97\u5404\u76f8\u5173\u6027\u65b9\u6cd5\uff0c\u53ef\u9009spearman\u6216pearson<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u4e3a\u4e86\u9632\u6b62\u6240\u6709\u6570\u636e\u805a\u96c6\u5728\u4e00\u8d77\uff0c\u8fd9\u662fjitter.num\u4f7f\u6570\u636e\u8fdb\u884c\u8f7b\u5fae\u6296\u52a8\uff0c\u8bbe\u7f6e\u4e3a0\u5219\u4e3a\u539f\u59cb\u6570\u636e\u7ed8\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># pos\u82e5\u4e3aTRUE\uff0c\u600e\u53ea\u4f7f\u7528\u4e24\u4e2a\u57fa\u56e0\u90fd\u8868\u8fbe\u7684\u7ec6\u80de\u8ba1\u7b97\u76f8\u5173\u6027\uff0c\u53ef\u907f\u514d\u597d\u591a\u7ec6\u80de\u4e2d\u5bf9\u5176\u4e2d\u4e00\u4e2a\u7ec6\u80de\u8868\u8fbe\u91cf\u4e3a0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">getScatterplot <- <span class=\"code-snippet__keyword\">function<\/span>(object, gene1, gene2, cor.method = <span class=\"code-snippet__string\">\"spearman\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">jitter.num = <span class=\"code-snippet__number\">0.15<\/span>, pos = <span class=\"code-snippet__literal\">TRUE<\/span>){<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span> (!gene1 %in% rownames(object)) {<\/span><\/code><code><span class=\"code-snippet_outer\">print(<span class=\"code-snippet__string\">\"gene1 was not found\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span> (!gene2 %in% rownames(object)) {<\/span><\/code><code><span class=\"code-snippet_outer\">print(<span class=\"code-snippet__string\">\"gene2 was not found\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">}<span class=\"code-snippet__keyword\">else<\/span>{<\/span><\/code><code><span class=\"code-snippet_outer\">exp.mat <- GetAssayData(object = object, assay = <span class=\"code-snippet__string\">\"RNA\"<\/span>) %>% .[<span class=\"code-snippet__built_in\">c<\/span>(gene1,gene2),] %>%<\/span><\/code><code><span class=\"code-snippet_outer\">as.matrix() %>% t() %>% as.data.frame()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span> (pos) {<\/span><\/code><code><span class=\"code-snippet_outer\">exp.mat <- exp.mat[which(exp.mat[,<span class=\"code-snippet__number\">1<\/span>] > <span class=\"code-snippet__number\">0<\/span> & exp.mat[,<span class=\"code-snippet__number\">2<\/span>] > <span class=\"code-snippet__number\">0<\/span>),]<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">plots <- ggplot(data=exp.mat, mapping = aes_string(x = gene1, y = gene2)) +<\/span><\/code><code><span class=\"code-snippet_outer\">geom_smooth(method = <span class=\"code-snippet__string\">'lm'<\/span>, se = <span class=\"code-snippet__built_in\">T<\/span>, color=<span class=\"code-snippet__string\">'red'<\/span>, size=<span class=\"code-snippet__number\">1<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">stat_cor(method = cor.method)+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_jitter(width = jitter.num, height = jitter.num, color = <span class=\"code-snippet__string\">\"black\"<\/span>, size = <span class=\"code-snippet__number\">1<\/span>, alpha=<span class=\"code-snippet__number\">1<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw()+<\/span><\/code><code><span class=\"code-snippet_outer\">theme(panel.grid=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.text=element_text(colour= <span class=\"code-snippet__string\">'black'<\/span>,size=<span class=\"code-snippet__number\">10<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text= element_text(colour= <span class=\"code-snippet__string\">'black'<\/span>,size=<span class=\"code-snippet__number\">10<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.line= element_line(colour= <span class=\"code-snippet__string\">'black'<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.border = element_rect(size = <span class=\"code-snippet__number\">1<\/span>, linetype = <span class=\"code-snippet__string\">\"solid\"<\/span>, colour = <span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.background=element_rect(fill=<span class=\"code-snippet__string\">\"white\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">return<\/span>(plots)<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8ba1\u7b97\u5171\u8868\u8fbe\u57fa\u56e0\u76f8\u5173\u6027\u5e76\u53ef\u89c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">getScatterplot(pbmc3k, gene1 = <span class=\"code-snippet__string\">\"CD3G\"<\/span>, gene2 = <span class=\"code-snippet__string\">\"CD247\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">jitter.num = <span class=\"code-snippet__number\">0.15<\/span>, pos = <span class=\"code-snippet__literal\">TRUE<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">theme(aspect.ratio = <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(<span class=\"code-snippet__string\">\"correlation_dotplot.pdf\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: center;\">\n<section style=\"transform: rotateZ(328deg) scale(0.6);transform-origin: center center;margin: -20px -19px;max-width: unset !important;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;margin: 14px 0% 11px;transform: translate3d(5px, 0px, 0px);display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 51px;height: 47px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgba(255, 255, 255, 0) rgb(252, 210, 125) rgb(252, 210, 125);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"transform: rotateZ(348deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: -37px;margin-right: 0%;margin-left: 0%;transform: translate3d(-12px, 0px, 0px);\">\n<section style=\"display: inline-block;width: 71px;height: 42px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px;border-radius: 100%;border-color: rgb(252, 210, 125) rgb(252, 210, 125) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;margin-left: -20px;\">\n<section style=\"transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 19px;letter-spacing: 0px;color: rgb(249, 172, 172);padding-right: 6px;padding-left: 6px;text-align: justify;\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 18px;\"><strong>\u7ed3\u679c\u6587\u4ef6<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;align-self: flex-start;\">\n<section style=\"transform: rotateZ(145deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 10px;vertical-align: top;border-bottom: 2px solid rgb(245, 172, 151);border-bottom-right-radius: 0px;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"transform: rotateZ(24deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-right: 0%;margin-bottom: 8px;margin-left: 0%;\">\n<section style=\"background-color: rgb(245, 172, 151);height: 2px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>1.UMAP_co-expression_matrix.pdf<\/strong><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u8be5\u7ed3\u679c\u56fe\u7247\u4e3aCD3G\u548cCD247\u8fd9\u4e24\u4e2aT\u7ec6\u80demarker\u57fa\u56e0\u8fdb\u884c\u57fa\u56e0\u5171\u8868\u8fbe\u53ef\u89c6\u5316\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.34444444444444444\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_FxlGbibxQsTnO7icwxzXJuW2B0zicw.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/oCSOtP61aEXahxt3mWw4QwXurdfBUNx155wa3ZDZoWAJh9DytqN1tBMfwa3FxlGbibxQsTnO7icwxzXJuW2B0zicw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>2.correlation_dotplot.pdf<\/strong><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u8be5\u7ed3\u679c\u56fe\u7247\u4e3a\u5171\u8868\u8fbe\u57fa\u56e0\u7684\u76f8\u5173\u6027\u6563\u70b9\u56fe\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9735376044568245\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_BF6Dic01R1N5sgCkxPMiaxad0MzI5g.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/oCSOtP61aEXahxt3mWw4QwXurdfBUNx1eGtv9yE6IbiaWAspzf8C9eXCDQNBF6Dic01R1N5sgCkxPMiaxad0MzI5g\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"718\" style=\"vertical-align: middle;width: 100%;\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u4eca\u5929\u5c0f\u5e08\u59b9\u5bf9\u5355\u7ec6\u80de\u57fa\u56e0\u5171\u8868\u8fbe\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\u5566\uff0c\u8fdb\u884c\u8be5\u5206\u6790\u9700\u8981\u719f\u7ec3\u638c\u63e1\u5355\u7ec6\u80de\u5206\u6790\u6d41\u7a0b\uff0c\u5355\u7ec6\u80de\u76f8\u5173\u5206\u6790\u5185\u5bb9\u53ef\u4ee5\u5c1d\u8bd5\u4f7f\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u4e91\u5e73\u53f0\u751f\u7269\u4fe1\u606f\u5206\u6790\u5c0f\u5de5\u5177\uff0c\u96f6\u4ee3\u7801\u5b8c\u6210\u5206\u6790\uff0c<span style=\"font-size: 16px;letter-spacing: 0.544px;\">\u5355\u7ec6\u80de\u5206\u6790\u76f8\u5173\u5c0f\u5de5\u5177\uff0c\u6b22\u8fce\u5c0f\u4f19\u4f34\u6765\u5c1d\u8bd5\u54df\uff01<\/span><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>\u4e91\u5e73\u53f0\u7f51\u5740\uff1a<\/strong><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">http:\/\/www.biocloudservice.com\/home.html<\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>\u5355\u7ec6\u80de\u5206\u6790\uff1a<\/strong><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">http:\/\/www.biocloudservice.com\/366\/366.php<\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>\u5355\u7ec6\u80de\u6570\u636e\u7ed8\u5236\u5c0f\u63d0\u7434\u56fe\uff1a<\/strong><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><span style=\"letter-spacing: 0.034em;\">http:\/\/www.biocloudservice.com\/788\/788.php<\/span><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>\u7ed8\u5236\u5355\u7ec6\u80detSNE\u56fe\uff1a<\/strong><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">http:\/\/www.biocloudservice.com\/229\/229.php<span style=\"letter-spacing: 0.034em;\"><\/span><\/p>\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;height: auto;align-self: center;margin-right: 10px;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(249, 172, 172);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);padding-right: 8px;padding-left: 8px;margin-right: 10px;\">\n<section style=\"text-align: justify;color: rgb(249, 172, 172);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">E<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);padding-right: 8px;padding-left: 8px;margin-right: 10px;\">\n<section style=\"text-align: justify;color: rgb(249, 172, 172);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">N<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);padding-right: 8px;padding-left: 8px;margin-right: 10px;\">\n<section style=\"text-align: justify;color: rgb(249, 172, 172);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">D<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 100 100 0%;height: auto;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(249, 172, 172);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-top: 10px;margin-bottom: 10px;letter-spacing: 0.578px;white-space: normal;text-align: center;line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 165.75px;height: auto;\">\n<p style=\"margin-right: 15px;margin-left: 15px;line-height: 1.75em;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.0018484288354899\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_cI8ibmMicsbTqIib079asIZ4uKuS6Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrHda7noEqDcniacdL1ZtgwuZceOSlFQGLmVsUCMtCaEdjYDkEiakicI8ibmMicsbTqIib079asIZ4uKuS6Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"541\" style=\"vertical-align: inherit;width: 135.75px;\"><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-top: 10px;margin-bottom: 10px;letter-spacing: 0.578px;white-space: normal;text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;border-style: solid;border-width: 1px;min-width: 5%;height: auto;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"padding-right: 10px;padding-left: 10px;display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;box-shadow: none;background-color: rgb(255, 255, 255);\">\n<section draggable=\"true\" style=\"text-align: justify;font-size: 15px;\">\n<p style=\"margin-right: 15px;margin-left: 15px;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;font-family: \u5fae\u8f6f\u96c5\u9ed1, "Microsoft YaHei";\">\u5e08\u59b9\u5fae\u4fe1<\/span><\/p>\n<\/section>\n<\/section>\n<section style=\"padding-right: 10px;padding-left: 10px;display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;background-color: rgb(240, 201, 207);\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);font-size: 15px;\">\n<p style=\"margin-right: 15px;margin-left: 15px;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;font-family: \u5fae\u8f6f\u96c5\u9ed1, "Microsoft YaHei";\">\u626b\u7801\u6dfb\u52a0<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd9\u4e5f\u53ef\u4ee5\u5b9e\u73b0\u5417\uff1f\u5355\u7ec6\u80de\u57fa\u56e0\u5171\u8868\u8fbe\u5206\u6790 \u5c0f\u5e08\u59b9 \u751f\u4fe1\u679c 2023-0 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55788"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=55788"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55788\/revisions"}],"predecessor-version":[{"id":55793,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/55788\/revisions\/55793"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=55788"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=55788"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=55788"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}