{"id":55794,"date":"2024-06-16T17:14:06","date_gmt":"2024-06-16T09:14:06","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55794"},"modified":"2024-06-16T17:14:06","modified_gmt":"2024-06-16T09:14:06","slug":"%e4%bf%9d%e5%a7%86%e7%ba%a7%e6%95%99%e7%a8%8b%ef%bc%81%e8%b7%9f%e7%9d%80%e5%b0%8f%e6%9e%9c%e4%bc%98%e9%9b%85%e7%9a%84%e7%bb%98%e5%88%b6%e4%bd%a0%e7%9a%84%e5%8d%95%e7%bb%86%e8%83%9e%e6%95%b0%e6%8d%ae-s","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55794","title":{"rendered":"\u4fdd\u59c6\u7ea7\u6559\u7a0b\uff01\u8ddf\u7740\u5c0f\u679c\u4f18\u96c5\u7684\u7ed8\u5236\u4f60\u7684\u5355\u7ec6\u80de\u6570\u636e-scCustomize\uff01"},"content":{"rendered":"


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\n<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner,body{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media{padding:20px;overflow:hidden;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}@media screen and (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media_content{overflow:hidden;color:#333;font-size:17px;word-wrap:break-word;-webkit-hyphens:auto;-ms-hyphens:auto;hyphens:auto;text-align:justify;position:relative;z-index:0}.rich_media_content *{max-width:100%!important;box-sizing:border-box!important;-webkit-box-sizing:border-box!important;word-wrap:break-word!important}.rich_media_content p{clear:both;min-height:1em}.rich_media_content em{font-style:italic}.rich_media_content fieldset{min-width:0}.rich_media_content .list-paddingleft-1,.rich_media_content .list-paddingleft-2,.rich_media_content .list-paddingleft-3{padding-left:2.2em}.rich_media_content .list-paddingleft-1 .list-paddingleft-2,.rich_media_content .list-paddingleft-2 .list-paddingleft-2,.rich_media_content .list-paddingleft-3 .list-paddingleft-2{padding-left:30px}.rich_media_content .list-paddingleft-1{padding-left:1.2em}.rich_media_content .list-paddingleft-3{padding-left:3.2em}.rich_media_content .code-snippet,.rich_media_content .code-snippet__fix{max-width:1000%!important}.rich_media_content .code-snippet *,.rich_media_content .code-snippet__fix *{max-width:1000%!important}.rich_media_title{font-size:22px;line-height:1.4;margin-bottom:13px;padding-bottom:13px;border-bottom:1px solid #e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag .code-snippet__attr{color:#0e9ce5}.code-snippet__string,.code-snippet__doctag{color:#d14}.code-snippet__title,.code-snippet__section,.code-snippet__selector-id{color:#d14}.code-snippet__subst{font-weight:normal}.code-snippet__type,.code-snippet__class .code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar 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weui-wa-hotarea\">\u539f\u521b  <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c  <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c  <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-12-18 20:00:32<\/em><\/div>\n<p><content><\/p>\n<div class=\"topic\">\n<p>\u6536\u5f55\u4e8e\u8bdd\u9898<\/p>\n<p><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3142740974448934918#wechat_redirect\" title=\"\u79d1\u7814\u4f5c\u56fe\" target=\"_blank\" rel=\"noopener\">#\u79d1\u7814\u4f5c\u56fe<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3142533467331887107#wechat_redirect\" title=\"\u5355\u7ec6\u80de\u6d4b\u5e8f\" target=\"_blank\" rel=\"noopener\">#\u5355\u7ec6\u80de\u6d4b\u5e8f<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2812010014583390210#wechat_redirect\" title=\"R\u4ee3\u7801\" target=\"_blank\" rel=\"noopener\">#R\u4ee3\u7801<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2821072943068839938#wechat_redirect\" title=\"\u751f\u4fe1\u7ed8\u56fe\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u7ed8\u56fe<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3142740974583152642#wechat_redirect\" title=\"\u751f\u4fe1\u8bfb\u56fe\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u8bfb\u56fe<\/a><\/p>\n<\/div>\n<p data-mpa-powered-by=\"yiban.io\"><span style=\"font-size: 15px;font-family: \u7b49\u7ebf;\"><span style=\"font-family: \u7b49\u7ebf;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100013881\" data-ratio=\"0.23333333333333334\" data-s=\"300,640\" data-type=\"jpeg\" data-w=\"1080\" style=\"font-family: \u5b8b\u4f53;font-size: 15px;letter-spacing: 0.578px;text-align: center;height: auto !important;\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_rE2v0O5WiaUDJjgv5bxK2G1ePHyH2g-38.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAysHY49vPGCraibakiaNq6hQgn8C5MfvVLQGxlNZPEia2PajKAzrE2v0O5WiaUDJjgv5bxK2G1ePHyH2g\/640?wx_fmt=jpeg'\" \/>\u770b\u8fc7\u5c0f\u679c\u524d\u51e0\u671f\u5bf9\u5355\u7ec6\u80de\u6807\u51c6\u6d41\u7a0b\u5904\u7406\u7684\u5c0f\u4f19\u4f34\u80af\u5b9a\u719f\u6089\u5355\u7ec6\u80de\u7684\u7ed3\u679c\uff0c\u4f46\u662f\u6709\u7684\u5c0f\u4f19\u4f34\u4e0d\u77e5\u9053\u5982\u4f55\u5c06\u7ed3\u679c\u53ef\u89c6\u5316\uff0c\u6216\u8005\u89c9\u5f97\u666e\u901a\u7684\u53ef\u89c6\u5316\u7ed3\u679c\u5df2\u7ecf\u6ee1\u8db3\u4e0d\u4e86\u5c0f\u4f19\u4f34\u7684\u80c3\u53e3\uff0c\u90a3\u4e48\u8fd9\u6b21\u5c0f\u679c\u7ed9\u5c0f\u4f19\u4f34\u5e26\u6765\u4e00\u671f\u4e13\u95e8\u7ed8\u5236\u5355\u7ec6\u80de\u76f8\u5173\u5206\u6790\u7684R\u5305sc<\/span>Customize\u7684\u4f7f\u7528\uff01<\/span><\/p>\n<p><span style=\"font-size: 15px;\"><span style=\"color: rgb(2, 30, 170);\"><strong><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u4e0b\u9762\u6211\u4eec\u5f00\u59cb\u5427<\/span><\/strong><\/span><\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u6211\u4eec\u8fd9\u91cc\u5148\u4e86\u89e3\u4e00\u4e0bscCustomize\u5305\uff0cscCustomize\u76ee\u7684\u5728\u5e2e\u52a9\u4f7f\u7528R\u5bf9\u5355\u7ec6\u80de\u6570\u636e\u8fdb\u884c\u53ef\u89c6\u5316\u548c\u5206\u6790\u3002\u5c0f\u4f19\u4f34\u8981\u6ce8\u610f\u4e0bscCustomize\u7684\u5b89\u88c5\u9700\u8981\u57284.0\u4ee5\u7684R\u7248\u672c\u4e0a\u3002<\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u6211\u4eec\u8fd9\u91cc\u4f7f\u7528\u7684\u6570\u636e\u662f\u524d\u51e0\u671f\u5c0f\u679c\u6559\u5927\u5bb6\u7684\u5355\u7ec6\u80de\u5904\u7406\u6d41\u7a0b\u6700\u7ec8\u7684\u7ed3\u679c\uff0c\u4e0d\u6e05\u695a\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u56de\u8fc7\u5934\u518d\u770b\u4e00\u4e0b\uff0c\u5c0f\u679c\u4e5f\u628a\u6570\u636e\u653e\u5728\u8fd9\u91cc\uff0c<\/span><\/p>\n<p><span style=\"color: rgb(2, 30, 170);font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><strong>\u6211\u4eec\u5148#\u52a0\u8f7d\u7ed8\u56fe\u6240\u9700\u8981\u7684\u5305<\/strong><\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u6b63\u5e38\u7684\u5b89\u88c5\u5c31\u53ef\u4ee5<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">#install.packages(\"scCustomize\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">library(tidyverse)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">library(patchwork)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">library(viridis)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">library(Seurat)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">library(scCustomize)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">library(qs)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-size: 15px;\"><span style=\"color: rgb(2, 30, 170);font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\"><strong>\u4e0b\u9762\u6211\u4eec\u52a0\u8f7d\u6570\u636e<\/strong><\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\"># \u57fa\u7840\u5206\u6790\u5f97\u5230\u7684\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">pbmc <- readRDS(\"pbmc3k_final.rds\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\"># \u5411pbmc\u6570\u636e\u4e2d\u6dfb\u52a0\u4e00\u4e9b\u968f\u673a\u5143\u6570\u636e\u53d8\u91cf\uff0c\u540e\u9762\u7ed8\u56fe\u4f7f\u7528<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">pbmc$sample_id <- sample(c(\"sample1\", \"sample2\", \"sample3\", \"sample4\"), size = ncol(pbmc),replace = TRUE) <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">pbmc$treatment <- sample(c(\"Treatment1\", \"Treatment2\"), size = ncol(pbmc),replace = TRUE)<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 16px;margin-bottom: 0px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(69, 107, 176);padding-right: 6px;padding-bottom: 6px;padding-left: 6px;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;transform: translate3d(19px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(255, 255, 255);padding: 23px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"margin-bottom: 0px;letter-spacing: 0.578px;text-wrap: wrap;font-size: 16px;background-color: rgb(255, 255, 255);\"><span style=\"font-size: 15px;\">\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d<span style=\"color: rgb(255, 41, 65);\">\u201c111\u201d<\/span>\u9886\u53d6\u672c\u7bc7\u4ee3\u7801\u3001\u57fa\u56e0\u96c6\u6216\u793a\u4f8b\u6570\u636e\u7b49\u6587\u4ef6\uff1b<\/span><\/p>\n<p style=\"margin-bottom: 0px;letter-spacing: 0.578px;text-wrap: wrap;font-size: 16px;background-color: rgb(255, 255, 255);\"><span style=\"text-decoration: underline;color: rgb(255, 41, 65);font-size: 15px;\"><em><strong>\u6587\u4ef6\u7f16\u53f7\uff1a231203<\/strong><\/em><\/span><\/p>\n<p style=\"margin-bottom: 0px;letter-spacing: 0.578px;text-wrap: wrap;font-size: 16px;background-color: rgb(255, 255, 255);\"><span style=\"font-size: 15px;\"><strong><span style=\"color: rgb(255, 41, 65);\">\u679c\u7c89\u798f\u5229<\/span><\/strong><span style=\"color: rgb(0, 128, 255);\"><strong>\uff1a\u751f\u4fe1\u4eba\u5fc5\u5907\u795e\u5668\u2014\u2014\u670d\u52a1\u5668<\/strong><\/span><br \/><\/span><\/p>\n<p style=\"margin-bottom: 0px;letter-spacing: 0.578px;text-wrap: wrap;font-size: 16px;background-color: rgb(255, 255, 255);\"><span style=\"font-size: 15px;\">\u5e73\u65f6\u751f\u4fe1\u5206\u6790\u5b66\u4e60\u4e2d\u6709\u8981\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u8054\u7cfb\u5c0f\u679c\u79df\u8d41\uff0c\u7c89\u4e1d\u798f\u5229\u90fd\u662f\u5e02\u573a\u8d85\u4f4e\u4ef7\u683c\uff0c\u8d76\u5feb\u627e\u5c0f\u679c\u9886\u53d6\u514d\u8d39\u7684\u8bd5\u7528\u8d26\u53f7\u5427\uff01<\/span><\/p>\n<p style=\"margin-bottom: 0px;letter-spacing: 0.578px;text-wrap: wrap;font-size: 16px;background-color: rgb(255, 255, 255);\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247495825&idx=2&sn=a22a72496a1c082ea5b827be94ce4d99&chksm=cf7f6054f808e94264e14bfed4359cab3fcaaca249952a9f761c37ece352dcd7108579788b89&scene=21#wechat_redirect\" textvalue=\"\u670d\u52a1\u5668\u4ef7\u683c\u914d\u7f6e\u8868\uff08\u70b9\u51fb\u94fe\u63a5\u67e5\u770b\uff09\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 15px;\" rel=\"noopener\"><span style=\"font-size: 15px;\">\u670d\u52a1\u5668\u4ef7\u683c\u914d\u7f6e\u8868\uff08\u70b9\u51fb\u94fe\u63a5\u67e5\u770b\uff09<\/span><\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"color: rgb(2, 30, 170);font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><strong>\u63a5\u4e0b\u6765\u6211\u4eec\u5bf9\u9ad8\u53d8\u57fa\u56e0\u8fdb\u884c\u7ed8\u5236<\/strong><\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100013728\" data-ratio=\"1.0045523520485584\" data-type=\"png\" data-w=\"659\" height=\"491.1099853515625\" width=\"488.8800048828125\" style=\"height: auto !important;\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_twiceAdiajkibBtVdnbDqyjxcYqnqQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyPT5Rdkc8iam7hJtPUwQPdqjz7dS2DRfsf10VPQLZwiae5iafwMFtwiceAdiajkibBtVdnbDqyjxcYqnqQ\/640?wx_fmt=png'\" \/><\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u4e0a\u8ff0\u53c2\u6570\u4e2dnum_features\uff1a\u57fa\u56e0\u6570\u91cf label\uff1a\u662f\u5426\u8fdb\u884c\u6807\u8bb0 repel\uff1a\u8fde\u63a5\u70b9\u548c\u6807\u7b7e y_axis_log\uff1a\u5bf9y\u8f74\u8fdb\u884c\u53d6\u5bf9\u6570 colors_use\uff1a\u8bbe\u7f6e\u989c\u8272 pt.size\uff1a\u70b9\u7684\u5927\u5c0f\u8bbe\u7f6e\u3002<\/span><\/p>\n<p><span style=\"color: rgb(2, 30, 170);font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><strong>\u63a5\u4e0b\u6765\u5c31\u662f\u7ed8\u5236\u4e00\u4e9b\u7279\u5f81\u56fe<\/strong><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\"># \u8bbe\u7f6e\u989c\u8272<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">pal <- viridis(n = 10, option = \"D\") <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">FeaturePlot_scCustom(seurat_object = pbmc, features = \"GNLY\", order = F)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100013732\" data-ratio=\"1.0698051948051948\" data-type=\"png\" data-w=\"616\" height=\"330.04998779296875\" style=\"font-family: \u7b49\u7ebf;font-size: 15px;letter-spacing: 0.034em;height: auto !important;\" width=\"308.510009765625\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_5IVbltLdeTD1BMwZ8v52y59W5Vib7A.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyPT5Rdkc8iam7hJtPUwQPdq7dNvmxibAVhesF4KI6CroEBeGU5IVbltLdeTD1BMwZ8v52y59W5Vib7A\/640?wx_fmt=png'\" \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"javascript\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">FeaturePlot_scCustom(seurat_object = pbmc, features = \"GNLY\")<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100013729\" data-ratio=\"1.0296875\" data-type=\"png\" data-w=\"640\" height=\"393.530029296875\" width=\"382.1600036621094\" style=\"height: auto !important;\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_PZ5iadB0bBAz02TevB90Ik3rtxhgsw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyPT5Rdkc8iam7hJtPUwQPdqxaHt6NjLIUw85b4RHiauaornpbPZ5iadB0bBAz02TevB90Ik3rtxhgsw\/640?wx_fmt=png'\" \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"javascript\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">FeaturePlot_scCustom(seurat_object = pbmc, features = \"GNLY\", colors_use = pal)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100013730\" data-ratio=\"1.0123839009287925\" data-type=\"png\" data-w=\"646\" height=\"356.239990234375\" width=\"351.8699951171875\" style=\"height: auto !important;\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_oU6oGPQOIOwovFiaCtGlncf6SZT6nw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyPT5Rdkc8iam7hJtPUwQPdq18ZiaF5TkMlGhU5WL0o2Pibic7QJoU6oGPQOIOwovFiaCtGlncf6SZT6nw\/640?wx_fmt=png'\" \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" data-type=\"png\" data-w=\"1080\" style=\"height: auto !important;\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_IWicymEetCc6Enf2BkUE4Yz1Ya5e5w-1.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyuYsqlJnQUJvnS2iciaDibtfbdV0VmHlriaSjYwjFPLp1BLjokHIWicymEetCc6Enf2BkUE4Yz1Ya5e5w\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u5176\u4e2d\u5462\uff0corder=F\uff0c\u53ef\u80fd\u4f1a\u5bfc\u81f4\u975e\u8868\u8fbe\u7ec6\u80de\u7ed8\u5236\u5728\u8868\u8fbe\u7ec6\u80de\u4e4b\u4e0a\u3002\u6709\u65f6order=T\u53ef\u80fd\u4f1a\u5bfc\u81f4\u7ed8\u5236\u7684\u70b9\u4e0d\u591f\u96c6\u4e2d\uff0c\u53ef\u4ee5\u7531\u7ed8\u5236\u57fa\u56e0\u7684\u60c5\u51b5\u6839\u636e\u9700\u8981\u6765\u8fdb\u884c\u8c03\u6574\uff0c\u5c0f\u4f19\u4f34\u8981\u6ce8\u610f\u4e0b\u54e6\uff01<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">al <- viridis(n = 10, option = \"C\", direction = -1)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">FeaturePlot_scCustom(seurat_object = pbmc, features = \"CD3E\", order = T,alpha_exp = 0.75,colors_use = pal)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100013731\" data-ratio=\"1.0125\" data-type=\"png\" data-w=\"640\" height=\"376.8699951171875\" width=\"372.20001220703125\" style=\"height: auto !important;\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_LSKu3DtlYARVCldXZc3nBjG1TbUO2Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyPT5Rdkc8iam7hJtPUwQPdqw8ey6ZibibOlx4zkF8tKkXTn2NLSKu3DtlYARVCldXZc3nBjG1TbUO2Q\/640?wx_fmt=png'\" \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"javascript\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">FeaturePlot_scCustom(seurat_object = pbmc, features = \"CD3E\", order = F,alpha_exp = 0.75,colors_use = pal)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100013734\" data-ratio=\"1.0124416796267497\" data-type=\"png\" data-w=\"643\" height=\"328.469970703125\" style=\"font-family: \u7b49\u7ebf;font-size: 15px;letter-spacing: 0.034em;height: auto !important;\" width=\"324.44000244140625\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_PWDbYl6ZQQbzsCgv6MFB37wiae3y0g.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyPT5Rdkc8iam7hJtPUwQPdqw1ZTOqMsItz685WNnd4qGEic9SPWDbYl6ZQQbzsCgv6MFB37wiae3y0g\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"font-size: 15px;\"><span style=\"font-family: \u7b49\u7ebf;letter-spacing: 0.034em;\">\u6211\u4eec\u6362\u4e2a\u597d\u770b\u7684\u989c\u8272\uff0c\u6ce8\u610f\u4e0b\u53c2\u6570alpha_exp\uff1a\u66f4\u6539\u70b9\u7684\u900f\u660e\u5ea6 colors_use\uff1a\u8bbe\u7f6e\u989c\u8272\u3002<\/span><\/span><\/p>\n<p><span style=\"font-size: 15px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u540e\u9762\u5230\u4e86\u6211\u4eec\u7684\u5173\u952e\u56fe\u4e4b\u4e00\u4e86\uff0c\u7ed8\u5236\u4e0b\u5bc6\u5ea6\u56fe\uff0c\u4f46\u662f\u5bc6\u5ea6\u56fe\u7684\u7ed8\u5236\u9700\u8981Nebulosa\u5305\u8fdb\u884c\u914d\u8272\uff0c\u53ef\u60dc\u7684\u662fNebulosa\u4ec5\u652f\u6301\u4f7f\u75285\u4e2aviridis\u8c03\u8272\u677f\u8fdb\u884c\u7ed8\u56fe\uff1a\u201cviridis\u201d\u3001\u201cmagma\u201d\u3001\u201ccividis\u201d\u3001\u201cinferno\u201d\u548c\u201cplasma\u201d\u3002\u4e0d\u8fc7\u6ca1\u4e8b\u591f\u6211\u4eec\u4f7f\u7528\u4e86\uff0c\u6765\u770b\u4e00\u4e0b<\/span>    <\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">library(Nebulosa)#\u5c0f\u4f19\u4f34\u9700\u8981\u4e0b\u8f7d\u4e00\u4e0b\u8fd9\u4e2a\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">\u4e00\u822c\u4f7f\u7528#BiocManager::install(\"Nebulosa\")\u5c31\u53ef\u4ee5<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">Plot_Density_Custom(seurat_object = pbmc, features = \"GNLY\")<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100013737\" data-ratio=\"1.0514018691588785\" data-type=\"png\" data-w=\"642\" height=\"449.8600158691406\" style=\"font-family: \u7b49\u7ebf;font-size: 15px;letter-spacing: 0.034em;height: auto !important;\" width=\"427.8599853515625\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_900BXQ8hbGpBicM9SZf2avqsqu7Y9g.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyPT5Rdkc8iam7hJtPUwQPdqWPyJ38Q7JVgxYNSKWt0I0Ftzk900BXQ8hbGpBicM9SZf2avqsqu7Y9g\/640?wx_fmt=png'\" \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"javascript\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">Plot_Density_Custom(seurat_object = pbmc, features = \"GNLY\", custom_palette = PurpleAndYellow(),)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100013736\" data-ratio=\"1.042654028436019\" data-type=\"png\" data-w=\"633\" height=\"415.739990234375\" width=\"398.75\" style=\"height: auto !important;\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_8zFQwrDadJ6B6icple8GIDeS543ibQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyPT5Rdkc8iam7hJtPUwQPdqUibzXWiaLeaibAZ0bhzpnEXzfdZBG8zFQwrDadJ6B6icple8GIDeS543ibQ\/640?wx_fmt=png'\" \/><\/span><\/p>\n<p><span style=\"color: rgb(2, 30, 170);font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><strong>\u6211\u4eec\u8fd8\u53ef\u4ee5\u4f7f\u7528Plot_Density_Joint_Only()\u53ef\u4ee5\u7ed8\u5236\u8054\u5408\u5bc6\u5ea6\u56fe\u3002<\/strong><\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100013735\" data-ratio=\"1.0365079365079366\" data-type=\"png\" data-w=\"630\" height=\"449.8599853515625\" width=\"434.030029296875\" style=\"height: auto !important;\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_d2n931HDhZTGibpibUbAgCumcPiaVA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyPT5Rdkc8iam7hJtPUwQPdquCpEIzrUkrya4oKJh9wlhwCiaBSOd2n931HDhZTGibpibUbAgCumcPiaVA\/640?wx_fmt=png'\" \/><\/span><span style=\"font-size: 15px;\">    <\/span><o:page><\/o:page><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 15px;\">\u662f\u4e0d\u662f\u4e0d\u4e00\u6837\u4e86\u5462<\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 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