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5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n <\/p>\n\u4eca\u5929\u5c0f\u82b1\u4e3a\u5927\u5bb6\u5e26\u6765\u7684\u5206\u4eab\u7070\u5e38\u7070\u5e38\u6709\u7528\uff0c\u5bf9\u4e8e\u5f88\u591a\u5c0f\u4f19\u4f34\u6765\u8bf4\uff0c\u514d\u75ab\u6d78\u6da6\u5206\u6790\u662f\u975e\u5e38\u719f\u6089\u7684\u5206\u6790\u5185\u5bb9\uff0c\u514d\u75ab\u6d78\u6da6\u5206\u6790\u65b9\u6cd5\u4e3b\u8981\u5305\u62ecxcell,MCP-counter,timer,quantiseq\uff0cepic\uff0ccibersort\uff0cestimate\u7b49\u65b9\u6cd5\uff0c\u4f46\u662f\u8fd9\u51e0\u79cd\u65b9\u6cd5\u90fd\u6709\u5404\u81ea\u7684R\u5305\u6765\u8fdb\u884c\u5206\u6790\uff0c\u6709\u51e0\u79cd\u65b9\u6cd5\u5c31\u9700\u8981\u5b89\u88c5\u548c\u5b66\u4e60\u51e0\u79cdR\u5305\uff0c\u5bf9\u5c0f\u767d\u548c\u521d\u5b66\u8005\u6765\u8bf4\u975e\u5e38\u4e0d\u53cb\u597d\uff0c\u9700\u8981\u82b1\u8d39\u5927\u91cf\u7684\u65f6\u95f4\uff0c\u57fa\u4e8e\u8fd9\u79cd\u60c5\u51b5\uff0c\u5c0f\u82b1\u4e3a\u5c0f\u4f19\u4f34\u63a8\u8350\u4e00\u4e2aR\u5305IOBR\uff0c\u8be5\u5305\u76f4\u63a5\u53ef\u4ee5\u5b8c\u6210\u591a\u79cd\u7b97\u6cd5\u7684\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\u6bcf\u4e2a\u7b97\u6cd5\u90fd\u53ea\u9700\u8981\u4e00\u53e5\u4ee3\u7801\u5b8c\u6210\u5206\u6790\uff0c\u975e\u5e38\u7b80\u5355\u5965\uff01\u5c0f\u82b1\u5f3a\u70c8\u63a8\u8350\uff0c\u63a5\u4e0b\u6765\u8ddf\u7740\u5c0f\u82b1\u5f00\u59cb\u4eca\u5929\u7684\u5206\u4eab\u5b66\u4e60\u3002<o:p><\/o:p><\/p>\n <\/p>\n1.<\/span>\u5b89\u88c5<\/span>IOBR<\/span>\u5305<\/span><\/strong><\/p>\n\u9996\u5148\u5c0f\u82b1\u4e3a\u5927\u5bb6\u7b80\u5355\u4ecb\u7ecd\u4e00\u4e0b\u5982\u4f55\u5b89\u88c5\u548c\u5b66\u4e60<\/span>IOBR<\/span>\u5305\uff0c\u5c0f\u82b1\u4e3a\u5c0f\u4f19\u4f34\u4eec\u9644\u4e0a\u7f51\u5740\uff1a<\/span>https:\/\/iobr.github.io\/IOBR\/IOBR-VIGNETTE.html<\/span>\uff0c\u60f3\u6df1\u5165\u5b66\u4e60\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u5230\u8be5\u7f51\u5740\u5b66\u4e60\u3002<\/span><o:p><\/o:p><\/span><\/p>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.37364620938628157\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_jYewziaT5bOERWUG51qIAEVjqpbv4A.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSGIZwst1kYiceOtpibtt8l6vfVDcjYewziaT5bOERWUG51qIAEVjqpbv4A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n#\u9700\u8981\u6ce8\u610f\u7684\u662f\u5b98\u7f51\u63a8\u8350\u4f7f\u7528github\u7684\u65b9\u6cd5\u6765\u5b89\u88c5\uff0c\u4f46\u662f\u7531\u4e8e\u7f51\u7edc\u95ee\u9898\uff0c\u901a\u8fc7github\u5b89\u88c5\u975e\u5e38\u975e\u5e38\u6162\uff0c\u4e00\u822c\u90fd\u4e0d\u80fd\u5b89\u88c5\u6210\u529f\uff0c\u56e0\u6b64\u5c0f\u82b1\u63a8\u8350\u76f4\u63a5\u4e0b\u8f7d\u5b89\u88c5\u5305\uff0c\u8fdb\u884c\u672c\u5730\u5b89\u88c5\uff0c\u5b89\u88c5\u901f\u5ea6\u975e\u5e38\u5feb\uff01\u5b89\u88c5\u5305\u53ef\u4ee5\u5230\u8be5\u7f51\u5740\u4e0b\u8f7d\uff1ahttps:\/\/github.com\/IOBR\/IOBR<\/span>\u3002<o:p><\/o:p><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code>#\u9996\u5148\u5b89\u88c5\u4f9d\u8d56\u7684R\u5305<\/span><\/span><\/code><code>if (!requireNamespace(\"BiocManager\", quietly = TRUE)) install.packages(\"BiocManager\"<\/span>)<\/span><\/code><code> <\/span><\/code><code>depens<-c('tibble'<\/span>, 'survival'<\/span>, 'survminer'<\/span>, 'sva'<\/span>, 'limma'<\/span>, \"DESeq2\"<\/span>,\"devtools\"<\/span>,<\/span><\/code><code> 'limSolve'<\/span>, 'GSVA'<\/span>, 'e1071'<\/span>, 'preprocessCore'<\/span>, 'ggplot2'<\/span>, \"biomaRt\"<\/span>,<\/span><\/code><code> 'ggpubr'<\/span>, \"devtools\"<\/span>, \"tidyHeatmap\"<\/span>, \"caret\"<\/span>, \"glmnet\"<\/span>, \"ppcor\"<\/span>, \"timeROC\"<\/span>,\"pracma\"<\/span>)<\/span><\/code><code>for<\/span>(i in<\/span> 1<\/span>:length<\/span>(depens)){<\/span><\/code><code> depen<-depens[i]<\/span><\/code><code> if<\/span> (!requireNamespace(depen, quietly = TRUE<\/span>))<\/span><\/code><code> BiocManager::install<\/span>(depen,update<\/span> = FALSE<\/span>)<\/span><\/code><code>}<\/span><\/code><code>#\u5b89\u88c5IOBR\u5305<\/span><\/span><\/code><code>install.packages(\"IOBR-master.tar.gz\"<\/span>,repos=NULL<\/span>,type<\/span>=\"source\"<\/span>)<\/span><\/code><code>#\u52a0\u8f7dIOBR\u5305<\/span><\/span><\/code><code>library<\/span>(IOBR)<\/span><\/code><\/pre>\n<\/section>\n2.<\/span>\u51e0\u79cd\u514d\u75ab\u7b97\u6cd5\u7684\u7b80\u5355\u4ecb\u7ecd<\/span><\/strong><o:p><\/o:p><\/span><\/p>\n#quanTIseq<\/span>\u7b97\u6cd5\u7b80\u5355\u4ecb\u7ecd<\/span><\/span><\/strong><o:p><\/o:p><\/span><\/p>\nquanTIseq<\/span>\u662f\u7528\u4e8e\u6839\u636e\u4eba\u7c7b<\/span>RNA-seq<\/span>\u6570\u636e\u91cf\u5316\u80bf\u7624\u514d\u75ab\u72b6\u51b5\uff0c\u901a\u8fc7\u53cd\u5377\u79ef\u91cf\u5316\u6837\u672c\u4e2d\u5b58\u5728\u7684<\/span>10<\/span>\u79cd\u4e0d\u540c\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u7684\u6bd4\u4f8b\u4ee5\u53ca\u5176\u4ed6\u672a\u8868\u5f81\u7ec6\u80de\u7684\u6bd4\u4f8b\u3002<\/span><\/span><o:p><\/o:p><\/span><\/p>\n <\/p>\n#xcell<\/span>\u7b97\u6cd5\u7b80\u5355\u4ecb\u7ecd<\/span><\/span><\/strong><o:p><\/o:p><\/span><\/p>\nxcell<\/span>\u662f\u57fa\u4e8e<\/span>ssGSEA<\/span>\u7684\u65b9\u6cd5\uff0c\u53ef\u6839\u636e<\/span>64<\/span>\u79cd\u514d\u75ab\u7ec6\u80de\u548c\u57fa\u8d28\u7ec6\u80de\u7c7b\u578b\u7684\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u8fdb\u884c\u7ec6\u80de\u7c7b\u578b\u5bcc\u96c6\u5206\u6790\u3002<\/span><\/span><o:p><\/o:p><\/span><\/p>\n <\/p>\n#TIMER<\/span>\u7b97\u6cd5\u7b80\u5355<\/span><\/span>\u4ecb\u7ecd<\/span><\/span><\/strong><o:p><\/o:p><\/span><\/p>\nTIMER<\/span>\u7528\u53cd\u5377\u79ef\u65b9\u6cd5\u4f30\u7b97<\/span>32<\/span>\u79cd\u764c\u75c7\u4e2d\u516d\u79cd\u514d\u75ab\u7ec6\u80de\u4e30\u5ea6\uff0c\u9700\u8981\u6ce8\u610f\u7684\u662f<\/span>TIMER<\/span>\u6709\u5728\u7ebf\u7684\u7f51\u7ad9\u53ef\u4ee5\u76f4\u63a5\u8fdb\u884c\u5206\u6790\u3002<\/span><\/span><o:p><\/o:p><\/span><\/p>\n <\/span><\/span><\/strong><\/p>\n#EPIC<\/span>\u7b97\u6cd5\u7b80\u5355<\/span><\/span>\u4ecb\u7ecd<\/span><\/span><\/strong><o:p><\/o:p><\/span><\/p>\nEPIC<\/span>\u4f7f\u7528\u7ea6\u675f\u6700\u5c0f\u4e8c\u4e58\u56de\u5f52\u5c06\u8d1f\u6027\u7ea6\u675f\u6761\u4ef6\u7eb3\u5165\u53cd\u5377\u79ef\u95ee\u9898\uff0c\u4ece\u5927\u91cf\u80bf\u7624\u6837\u672c\u7684\u8868\u8fbe\u8c31\u6570\u636e\u8fdb\u884c\u5206\u6790\u3002<\/span><\/span><o:p><\/o:p><\/span><\/p>\n <\/p>\n3.<\/span>\u8bfb\u53d6\u8f93\u5165\u6570\u636e<\/span><\/span><\/strong><o:p><\/o:p><\/span><\/p>\n#EXP.txt<\/span>\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\uff0c\u884c\u540d\u4e3a\u57fa\u56e0\u540d\uff0c\u5217\u540d\u4e3a\u6837\u672c\u540d<\/span><\/span><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>dat <-<\/span> read.table<\/span>(\"EXP.txt<\/span>\",sep<\/span> = \"t\"<\/span>,row.names<\/span> = 1,header<\/span> = T,stringsAsFactors<\/span> = F,check.names<\/span> = F)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2694394213381555\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_ibaYQwiblnkHxWldYicEygicZqRFBw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSqRwvL0TWibHhdLscSC4WRO4hQZiaqribaYQwiblnkHxWldYicEygicZqRFBw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"553\" style=\"\" \/><\/p>\n <\/p>\n <\/p>\n4.\u901a\u8fc7IOBR\u5305\u91c7\u7528\u591a\u79cd\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<o:p><\/o:p><\/p>\n#\u5229\u7528CIBERSORT\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>cibersort<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"cibersort\"<\/span>, arrays<\/span> = FALSE,<\/span> perm<\/span> = 200<\/span> )<\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.24007220216606498\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_oYPPic4bOejXs5zt6EiaRclP4eOA3Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSBWUOn8MiaKUqd0ltsnLCriccTfTeoYPPic4bOejXs5zt6EiaRclP4eOA3Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n<o:p><\/o:p><\/span><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n#\u5229\u7528EPIC\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>epic<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"epic\"<\/span>, arrays<\/span> = FALSE)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<o:p><\/o:p><\/span><\/p>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.27256317689530685\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_UfAGbTQJAxzadwlndHIllyqnGXLbqg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSxFqaDgrXpT8HlH3ZW5vQphV0UfAGbTQJAxzadwlndHIllyqnGXLbqg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n#\u5229\u7528mcpcounter\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>mcp<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"mcpcounter\"<\/span>)<\/span><\/span><\/code><\/pre>\n<\/section>\n<o:p><\/o:p><\/span><\/p>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2423146473779385\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_xKVoGviawehickpLro06V178zWhraA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSusERhsyT5CEeMHEGB1ricu3QlibKxKVoGviawehickpLro06V178zWhraA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"553\" style=\"\" \/><\/p>\n <\/p>\n#\u5229\u7528xCELL\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<o:p><\/o:p><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>xcell<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"xcell\"<\/span>,arrays<\/span> = FALSE)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2545126353790614\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_eG9I6zCAMzHF5j5QEKHN7nicibLwuw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZS7micKVG3G3Q7Lj49doMjfKs61ureG9I6zCAMzHF5j5QEKHN7nicibLwuw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n#\u5229\u7528estimate\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>estimate<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"estimate\"<\/span>)<\/span><\/span><\/code><\/pre>\n<\/section>\n<o:p><\/o:p><\/span><\/p>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.24548736462093862\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_KG64ppw3vj10El2YvTicb9icc8xiaA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSNJ7sYzdsL4x0vR6RNPddicVbqmnKKG64ppw3vj10El2YvTicb9icc8xiaA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n#<\/span>\u5229\u7528<\/span>TIMER<\/span>\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<\/span><\/span><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>timer<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"timer\"<\/span>, group_list<\/span> = rep(<\/span>\"stad<\/span>\",dim<\/span>(dat<\/span>)[2<\/span>]))<\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2545126353790614\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_uRWSfvLmdJ7KyJu3yeTxIn3KdgyDkw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSM6iaw0yM5CecibEpEAOCibnIS6xuRWSfvLmdJ7KyJu3yeTxIn3KdgyDkw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>#\u5229\u7528quanTIseq\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<\/span><\/code><code>quantiseq<-deconvo_tme(eset<\/span> = dat,<\/span> tumor<\/span> = TRUE,<\/span> arrays<\/span> = FALSE,<\/span> scale_mrna<\/span> = TRUE,<\/span> method<\/span> = \"quantiseq\"<\/span>)<\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.27075812274368233\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_x0lfvCDFfLEicf5aXpaaKavJlM4WeQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSibFiclHpTszj3GM94h72223nD6vx0lfvCDFfLEicf5aXpaaKavJlM4WeQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code>#\u8fde\u63a57\u79cd\u7b97\u6cd5\u514d\u75ab\u5206\u6790\u7ed3\u679c<\/span><\/span><\/code><code>tme_combine<-cibersort %>% <\/span><\/code><code>inner_join(.,mcp,by<\/span> = \"ID\"<\/span>) %>% <\/span><\/code><code>inner_join(.,xcell,by<\/span> = \"ID\"<\/span>) %>%<\/span><\/code><code>inner_join(.,epic,by<\/span> = \"ID\"<\/span>) %>% <\/span><\/code><code>inner_join(.,estimate,by<\/span> = \"ID\"<\/span>) %>% <\/span><\/code><code>inner_join(.,timer,by<\/span> = \"ID\"<\/span>) %>% <\/span><\/code><code>inner_join(.,quantiseq,by<\/span> = \"ID\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<\/span><o:p><\/o:p><\/span><\/p>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2996389891696751\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_6jXG6H4eePR1DcEiceXYVUicrKwS0Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSE38JY8glZdg7mMBPPibDm7xVn106jXG6H4eePR1DcEiceXYVUicrKwS0Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n#IPS<\/span>\u514d\u75ab\u8868\u578b\u8bc4\u5206\u8ba1\u7b97<\/span><\/span><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>ips<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"ips\"<\/span>, plot<\/span>= FALSE)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<o:p><\/o:p><\/span><\/p>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.30505415162454874\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_cNo7R4jLAv1Y0D56Cg8N5szxF6Lm3Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSan5abH99AKzzwq2JZ9bHnB9FicNo7R4jLAv1Y0D56Cg8N5szxF6Lm3Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n <\/p>\n<\/section>\n <\/p>\n\u6700\u7ec8\u5c0f\u82b1\u6210\u529f\u7684\u5229\u7528IOBR\u5305\u7b97\u6cd5\u8fdb\u884c\u4e86\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\u5305\u62ecCIBERSORT\uff0cEPIC\uff0cmcpcounter\uff0cxCELL\uff0cestimate\uff0cTIMER\uff0cquanTIseq 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style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(201, 229, 70);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n <\/p>\n\u4eca\u5929\u5c0f\u82b1\u4e3a\u5927\u5bb6\u5e26\u6765\u7684\u5206\u4eab\u7070\u5e38\u7070\u5e38\u6709\u7528\uff0c\u5bf9\u4e8e\u5f88\u591a\u5c0f\u4f19\u4f34\u6765\u8bf4\uff0c\u514d\u75ab\u6d78\u6da6\u5206\u6790\u662f\u975e\u5e38\u719f\u6089\u7684\u5206\u6790\u5185\u5bb9\uff0c\u514d\u75ab\u6d78\u6da6\u5206\u6790\u65b9\u6cd5\u4e3b\u8981\u5305\u62ecxcell,MCP-counter,timer,quantiseq\uff0cepic\uff0ccibersort\uff0cestimate\u7b49\u65b9\u6cd5\uff0c\u4f46\u662f\u8fd9\u51e0\u79cd\u65b9\u6cd5\u90fd\u6709\u5404\u81ea\u7684R\u5305\u6765\u8fdb\u884c\u5206\u6790\uff0c\u6709\u51e0\u79cd\u65b9\u6cd5\u5c31\u9700\u8981\u5b89\u88c5\u548c\u5b66\u4e60\u51e0\u79cdR\u5305\uff0c\u5bf9\u5c0f\u767d\u548c\u521d\u5b66\u8005\u6765\u8bf4\u975e\u5e38\u4e0d\u53cb\u597d\uff0c\u9700\u8981\u82b1\u8d39\u5927\u91cf\u7684\u65f6\u95f4\uff0c\u57fa\u4e8e\u8fd9\u79cd\u60c5\u51b5\uff0c\u5c0f\u82b1\u4e3a\u5c0f\u4f19\u4f34\u63a8\u8350\u4e00\u4e2aR\u5305IOBR\uff0c\u8be5\u5305\u76f4\u63a5\u53ef\u4ee5\u5b8c\u6210\u591a\u79cd\u7b97\u6cd5\u7684\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\u6bcf\u4e2a\u7b97\u6cd5\u90fd\u53ea\u9700\u8981\u4e00\u53e5\u4ee3\u7801\u5b8c\u6210\u5206\u6790\uff0c\u975e\u5e38\u7b80\u5355\u5965\uff01\u5c0f\u82b1\u5f3a\u70c8\u63a8\u8350\uff0c\u63a5\u4e0b\u6765\u8ddf\u7740\u5c0f\u82b1\u5f00\u59cb\u4eca\u5929\u7684\u5206\u4eab\u5b66\u4e60\u3002<o:p><\/o:p><\/p>\n <\/p>\n1.<\/span>\u5b89\u88c5<\/span>IOBR<\/span>\u5305<\/span><\/strong><\/p>\n\u9996\u5148\u5c0f\u82b1\u4e3a\u5927\u5bb6\u7b80\u5355\u4ecb\u7ecd\u4e00\u4e0b\u5982\u4f55\u5b89\u88c5\u548c\u5b66\u4e60<\/span>IOBR<\/span>\u5305\uff0c\u5c0f\u82b1\u4e3a\u5c0f\u4f19\u4f34\u4eec\u9644\u4e0a\u7f51\u5740\uff1a<\/span>https:\/\/iobr.github.io\/IOBR\/IOBR-VIGNETTE.html<\/span>\uff0c\u60f3\u6df1\u5165\u5b66\u4e60\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u5230\u8be5\u7f51\u5740\u5b66\u4e60\u3002<\/span><o:p><\/o:p><\/span><\/p>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.37364620938628157\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_jYewziaT5bOERWUG51qIAEVjqpbv4A.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSGIZwst1kYiceOtpibtt8l6vfVDcjYewziaT5bOERWUG51qIAEVjqpbv4A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n#\u9700\u8981\u6ce8\u610f\u7684\u662f\u5b98\u7f51\u63a8\u8350\u4f7f\u7528github\u7684\u65b9\u6cd5\u6765\u5b89\u88c5\uff0c\u4f46\u662f\u7531\u4e8e\u7f51\u7edc\u95ee\u9898\uff0c\u901a\u8fc7github\u5b89\u88c5\u975e\u5e38\u975e\u5e38\u6162\uff0c\u4e00\u822c\u90fd\u4e0d\u80fd\u5b89\u88c5\u6210\u529f\uff0c\u56e0\u6b64\u5c0f\u82b1\u63a8\u8350\u76f4\u63a5\u4e0b\u8f7d\u5b89\u88c5\u5305\uff0c\u8fdb\u884c\u672c\u5730\u5b89\u88c5\uff0c\u5b89\u88c5\u901f\u5ea6\u975e\u5e38\u5feb\uff01\u5b89\u88c5\u5305\u53ef\u4ee5\u5230\u8be5\u7f51\u5740\u4e0b\u8f7d\uff1ahttps:\/\/github.com\/IOBR\/IOBR<\/span>\u3002<o:p><\/o:p><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code>#\u9996\u5148\u5b89\u88c5\u4f9d\u8d56\u7684R\u5305<\/span><\/span><\/code><code>if (!requireNamespace(\"BiocManager\", quietly = TRUE)) install.packages(\"BiocManager\"<\/span>)<\/span><\/code><code> <\/span><\/code><code>depens<-c('tibble'<\/span>, 'survival'<\/span>, 'survminer'<\/span>, 'sva'<\/span>, 'limma'<\/span>, \"DESeq2\"<\/span>,\"devtools\"<\/span>,<\/span><\/code><code> 'limSolve'<\/span>, 'GSVA'<\/span>, 'e1071'<\/span>, 'preprocessCore'<\/span>, 'ggplot2'<\/span>, \"biomaRt\"<\/span>,<\/span><\/code><code> 'ggpubr'<\/span>, \"devtools\"<\/span>, \"tidyHeatmap\"<\/span>, \"caret\"<\/span>, \"glmnet\"<\/span>, \"ppcor\"<\/span>, \"timeROC\"<\/span>,\"pracma\"<\/span>)<\/span><\/code><code>for<\/span>(i in<\/span> 1<\/span>:length<\/span>(depens)){<\/span><\/code><code> depen<-depens[i]<\/span><\/code><code> if<\/span> (!requireNamespace(depen, quietly = TRUE<\/span>))<\/span><\/code><code> BiocManager::install<\/span>(depen,update<\/span> = FALSE<\/span>)<\/span><\/code><code>}<\/span><\/code><code>#\u5b89\u88c5IOBR\u5305<\/span><\/span><\/code><code>install.packages(\"IOBR-master.tar.gz\"<\/span>,repos=NULL<\/span>,type<\/span>=\"source\"<\/span>)<\/span><\/code><code>#\u52a0\u8f7dIOBR\u5305<\/span><\/span><\/code><code>library<\/span>(IOBR)<\/span><\/code><\/pre>\n<\/section>\n2.<\/span>\u51e0\u79cd\u514d\u75ab\u7b97\u6cd5\u7684\u7b80\u5355\u4ecb\u7ecd<\/span><\/strong><o:p><\/o:p><\/span><\/p>\n#quanTIseq<\/span>\u7b97\u6cd5\u7b80\u5355\u4ecb\u7ecd<\/span><\/span><\/strong><o:p><\/o:p><\/span><\/p>\nquanTIseq<\/span>\u662f\u7528\u4e8e\u6839\u636e\u4eba\u7c7b<\/span>RNA-seq<\/span>\u6570\u636e\u91cf\u5316\u80bf\u7624\u514d\u75ab\u72b6\u51b5\uff0c\u901a\u8fc7\u53cd\u5377\u79ef\u91cf\u5316\u6837\u672c\u4e2d\u5b58\u5728\u7684<\/span>10<\/span>\u79cd\u4e0d\u540c\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u7684\u6bd4\u4f8b\u4ee5\u53ca\u5176\u4ed6\u672a\u8868\u5f81\u7ec6\u80de\u7684\u6bd4\u4f8b\u3002<\/span><\/span><o:p><\/o:p><\/span><\/p>\n <\/p>\n#xcell<\/span>\u7b97\u6cd5\u7b80\u5355\u4ecb\u7ecd<\/span><\/span><\/strong><o:p><\/o:p><\/span><\/p>\nxcell<\/span>\u662f\u57fa\u4e8e<\/span>ssGSEA<\/span>\u7684\u65b9\u6cd5\uff0c\u53ef\u6839\u636e<\/span>64<\/span>\u79cd\u514d\u75ab\u7ec6\u80de\u548c\u57fa\u8d28\u7ec6\u80de\u7c7b\u578b\u7684\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u8fdb\u884c\u7ec6\u80de\u7c7b\u578b\u5bcc\u96c6\u5206\u6790\u3002<\/span><\/span><o:p><\/o:p><\/span><\/p>\n <\/p>\n#TIMER<\/span>\u7b97\u6cd5\u7b80\u5355<\/span><\/span>\u4ecb\u7ecd<\/span><\/span><\/strong><o:p><\/o:p><\/span><\/p>\nTIMER<\/span>\u7528\u53cd\u5377\u79ef\u65b9\u6cd5\u4f30\u7b97<\/span>32<\/span>\u79cd\u764c\u75c7\u4e2d\u516d\u79cd\u514d\u75ab\u7ec6\u80de\u4e30\u5ea6\uff0c\u9700\u8981\u6ce8\u610f\u7684\u662f<\/span>TIMER<\/span>\u6709\u5728\u7ebf\u7684\u7f51\u7ad9\u53ef\u4ee5\u76f4\u63a5\u8fdb\u884c\u5206\u6790\u3002<\/span><\/span><o:p><\/o:p><\/span><\/p>\n <\/span><\/span><\/strong><\/p>\n#EPIC<\/span>\u7b97\u6cd5\u7b80\u5355<\/span><\/span>\u4ecb\u7ecd<\/span><\/span><\/strong><o:p><\/o:p><\/span><\/p>\nEPIC<\/span>\u4f7f\u7528\u7ea6\u675f\u6700\u5c0f\u4e8c\u4e58\u56de\u5f52\u5c06\u8d1f\u6027\u7ea6\u675f\u6761\u4ef6\u7eb3\u5165\u53cd\u5377\u79ef\u95ee\u9898\uff0c\u4ece\u5927\u91cf\u80bf\u7624\u6837\u672c\u7684\u8868\u8fbe\u8c31\u6570\u636e\u8fdb\u884c\u5206\u6790\u3002<\/span><\/span><o:p><\/o:p><\/span><\/p>\n <\/p>\n3.<\/span>\u8bfb\u53d6\u8f93\u5165\u6570\u636e<\/span><\/span><\/strong><o:p><\/o:p><\/span><\/p>\n#EXP.txt<\/span>\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\uff0c\u884c\u540d\u4e3a\u57fa\u56e0\u540d\uff0c\u5217\u540d\u4e3a\u6837\u672c\u540d<\/span><\/span><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>dat <-<\/span> read.table<\/span>(\"EXP.txt<\/span>\",sep<\/span> = \"t\"<\/span>,row.names<\/span> = 1,header<\/span> = T,stringsAsFactors<\/span> = F,check.names<\/span> = F)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2694394213381555\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_ibaYQwiblnkHxWldYicEygicZqRFBw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSqRwvL0TWibHhdLscSC4WRO4hQZiaqribaYQwiblnkHxWldYicEygicZqRFBw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"553\" style=\"\" \/><\/p>\n <\/p>\n <\/p>\n4.\u901a\u8fc7IOBR\u5305\u91c7\u7528\u591a\u79cd\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<o:p><\/o:p><\/p>\n#\u5229\u7528CIBERSORT\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>cibersort<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"cibersort\"<\/span>, arrays<\/span> = FALSE,<\/span> perm<\/span> = 200<\/span> )<\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.24007220216606498\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_oYPPic4bOejXs5zt6EiaRclP4eOA3Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSBWUOn8MiaKUqd0ltsnLCriccTfTeoYPPic4bOejXs5zt6EiaRclP4eOA3Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n<o:p><\/o:p><\/span><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n#\u5229\u7528EPIC\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>epic<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"epic\"<\/span>, arrays<\/span> = FALSE)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<o:p><\/o:p><\/span><\/p>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.27256317689530685\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_UfAGbTQJAxzadwlndHIllyqnGXLbqg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSxFqaDgrXpT8HlH3ZW5vQphV0UfAGbTQJAxzadwlndHIllyqnGXLbqg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n#\u5229\u7528mcpcounter\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>mcp<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"mcpcounter\"<\/span>)<\/span><\/span><\/code><\/pre>\n<\/section>\n<o:p><\/o:p><\/span><\/p>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2423146473779385\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_xKVoGviawehickpLro06V178zWhraA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSusERhsyT5CEeMHEGB1ricu3QlibKxKVoGviawehickpLro06V178zWhraA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"553\" style=\"\" \/><\/p>\n <\/p>\n#\u5229\u7528xCELL\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<o:p><\/o:p><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>xcell<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"xcell\"<\/span>,arrays<\/span> = FALSE)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2545126353790614\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_eG9I6zCAMzHF5j5QEKHN7nicibLwuw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZS7micKVG3G3Q7Lj49doMjfKs61ureG9I6zCAMzHF5j5QEKHN7nicibLwuw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n#\u5229\u7528estimate\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>estimate<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"estimate\"<\/span>)<\/span><\/span><\/code><\/pre>\n<\/section>\n<o:p><\/o:p><\/span><\/p>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.24548736462093862\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_KG64ppw3vj10El2YvTicb9icc8xiaA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSNJ7sYzdsL4x0vR6RNPddicVbqmnKKG64ppw3vj10El2YvTicb9icc8xiaA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n#<\/span>\u5229\u7528<\/span>TIMER<\/span>\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<\/span><\/span><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>timer<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"timer\"<\/span>, group_list<\/span> = rep(<\/span>\"stad<\/span>\",dim<\/span>(dat<\/span>)[2<\/span>]))<\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2545126353790614\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_uRWSfvLmdJ7KyJu3yeTxIn3KdgyDkw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSM6iaw0yM5CecibEpEAOCibnIS6xuRWSfvLmdJ7KyJu3yeTxIn3KdgyDkw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>#\u5229\u7528quanTIseq\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<\/span><\/code><code>quantiseq<-deconvo_tme(eset<\/span> = dat,<\/span> tumor<\/span> = TRUE,<\/span> arrays<\/span> = FALSE,<\/span> scale_mrna<\/span> = TRUE,<\/span> method<\/span> = \"quantiseq\"<\/span>)<\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.27075812274368233\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_x0lfvCDFfLEicf5aXpaaKavJlM4WeQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSibFiclHpTszj3GM94h72223nD6vx0lfvCDFfLEicf5aXpaaKavJlM4WeQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code>#\u8fde\u63a57\u79cd\u7b97\u6cd5\u514d\u75ab\u5206\u6790\u7ed3\u679c<\/span><\/span><\/code><code>tme_combine<-cibersort %>% <\/span><\/code><code>inner_join(.,mcp,by<\/span> = \"ID\"<\/span>) %>% <\/span><\/code><code>inner_join(.,xcell,by<\/span> = \"ID\"<\/span>) %>%<\/span><\/code><code>inner_join(.,epic,by<\/span> = \"ID\"<\/span>) %>% <\/span><\/code><code>inner_join(.,estimate,by<\/span> = \"ID\"<\/span>) %>% <\/span><\/code><code>inner_join(.,timer,by<\/span> = \"ID\"<\/span>) %>% <\/span><\/code><code>inner_join(.,quantiseq,by<\/span> = \"ID\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<\/span><o:p><\/o:p><\/span><\/p>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2996389891696751\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_6jXG6H4eePR1DcEiceXYVUicrKwS0Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSE38JY8glZdg7mMBPPibDm7xVn106jXG6H4eePR1DcEiceXYVUicrKwS0Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n#IPS<\/span>\u514d\u75ab\u8868\u578b\u8bc4\u5206\u8ba1\u7b97<\/span><\/span><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>ips<-deconvo_tme(eset<\/span> = dat,<\/span> method<\/span> = \"ips\"<\/span>, plot<\/span>= FALSE)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<o:p><\/o:p><\/span><\/p>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.30505415162454874\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_cNo7R4jLAv1Y0D56Cg8N5szxF6Lm3Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSYz1PI4TtbNOmzwlWtgGXZSan5abH99AKzzwq2JZ9bHnB9FicNo7R4jLAv1Y0D56Cg8N5szxF6Lm3Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" \/><\/p>\n <\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n <\/p>\n<\/section>\n <\/p>\n\u6700\u7ec8\u5c0f\u82b1\u6210\u529f\u7684\u5229\u7528IOBR\u5305\u7b97\u6cd5\u8fdb\u884c\u4e86\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\u5305\u62ecCIBERSORT\uff0cEPIC\uff0cmcpcounter\uff0cxCELL\uff0cestimate\uff0cTIMER\uff0cquanTIseq 7\u79cd\u7b97\u6cd5,\u6700\u540e\u8fd8\u8ba1\u7b97\u4e86\u514d\u75ab\u8868\u578b\u8bc4\u5206(IPS),\u8be5\u5305\u5206\u6790\u6548\u679c\u975e\u5e38\u4e0d\u9519\uff0c\u6b22\u8fce\u5927\u5bb6\u548c\u5c0f\u82b1\u4e00\u8d77\u8ba8\u8bba\u5b66\u4e60\u5440\uff01\u514d\u75ab\u6d78\u6da6\u76f8\u5173\u5206\u6790\u5185\u5bb9\uff0c\u4f8b\u5982\u5355\u6837\u672c\u5bcc\u96c6\u7b97\u6cd5\u5206\u6790\u514d\u75ab\u6d78\u6da6\u4e30\u5ea6(http:\/\/www.biocloudservice.com\/106\/106.php)\uff0c\u8ba1\u7b9764\u79cd\u514d\u75ab\u7ec6\u80de\u76f8\u5bf9<\/span><\/p>\n\u542b\u91cf(http:\/\/www.biocloudservice.com\/107\/107.php)\uff0cpanImmune\u514d\u75ab\u6d78\u6da6\u5206\u6790(http:\/\/www.biocloudservice.com\/782\/782.php)\uff0c\u57fa\u4e8e\u57fa\u56e0\u8868\u8fbe\u8c31\u4f30\u7b97\u6837\u672c\u514d\u75ab\u57fa\u8d28\u8bc4\u5206\u548c\u80bf\u7624\u7eaf\u5ea6(http:\/\/www.biocloudservice.com\/114\/114.php)\u7b49\u90fd\u53ef\u4ee5\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u4e91\u5e73\u53f0\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\uff0c\u4e00\u952e\u5b8c\u6210\u8be5\u5206\u6790\u5965\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u5c1d\u8bd5\u5965\uff0c\u7f51\u5740\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002<\/span>\u4eca\u5929\u5c0f\u82b1\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\uff0c\u4e0b\u671f\u5728\u89c1\u5965\u3002<o:p><\/o:p><\/span><\/p>\n<o:p><\/o:p><\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;font-size: 16px;letter-spacing: 0.578px;white-space: normal;text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;vertical-align: middle;width: 40.6667px;align-self: center;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7434944237918215\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_DsGlqEbyCicrBvWND6p21siabffBDA.gif\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_gif\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibyrlnYHKpXHqd0HWVnWKG2jCwfibDsGlqEbyCicrBvWND6p21siabffBDA\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"269\" style=\"vertical-align: middle;width: 40.6667px;\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 177.948px;align-self: center;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"178\" data-cropsely1=\"0\" data-cropsely2=\"53\" data-ratio=\"1\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/13_g3QkgKRyGrcXuVcRuy0TxF7QbOA5lQ.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibOCAs16wqcbibQF52pia3AsicicM2g3QkgKRyGrcXuVcRuy0TxF7QbOA5lQ\/640?wx_fmt=jpeg'\" data-type=\"gif\" data-w=\"796\" style=\"height: 178px;vertical-align: middle;width: 178px;\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 40.6667px;align-self: center;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7434944237918215\" data-s=\"300,640\" src=\"http:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/14_rL4tsfT96vpzIsHBrsoQqDuUUldFAg.gif\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_gif\/KDvR2NrGicSYC5thibKicIZkAiaibGdPcbFaibkGkZ7Bia7bSTtFPQKYrBvKcVArL4tsfT96vpzIsHBrsoQqDuUUldFAg\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"269\" style=\"vertical-align: middle;width: 40.6667px;\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;font-size: 16px;letter-spacing: 0.578px;white-space: normal;text-align: center;\">\n\u957f\u6309\u8bc6\u522b\u4e8c\u7ef4\u7801\u5173\u6ce8\u6211\u4eec\u54df<\/strong><\/p>\n<\/section>\n<section style=\"text-align: justify;line-height: 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