{"id":55857,"date":"2024-06-16T17:16:18","date_gmt":"2024-06-16T09:16:18","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55857"},"modified":"2024-06-16T17:16:18","modified_gmt":"2024-06-16T09:16:18","slug":"3%e5%88%86%e9%92%9f%e8%af%bb%e6%87%82%e6%9c%80%e5%8f%97%e6%ac%a2%e8%bf%8e%e7%9a%84%e5%8d%95%e7%bb%86%e8%83%9e%e6%b5%8b%e5%ba%8f%e5%b9%b3%e5%8f%b010xgenomics%e7%bb%86%e8%83%9e%e5%88%86%e9%80%89","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=55857","title":{"rendered":"3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f"},"content":{"rendered":"


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style=\"font-size: 14px;letter-spacing: 0.034em;\">\u5355\u7ec6\u80de\u6d4b\u5e8f\uff08Single cell RNA sequencing\uff0cscRNA-seq\uff09\u662f\u5728\u5355\u4e2a\u7ec6\u80de\u6c34\u5e73\u8fdb\u884c\u8f6c\u5f55\u7ec4\u5b66\u5206\u6790\u7684\u6280\u672f\u3002\u5728\u5355\u4e2a\u7ec6\u80de\u7684\u5206\u8fa8\u7387\u4e0b\uff0c\u79d1\u5b66\u5bb6\u80fd\u771f\u6b63\u7684\u5b9a\u4e49\u7ec6\u80de\u7c7b\u578b\u7279\u5b9a\u7684\u57fa\u56e0\u8868\u8fbe\uff0c\u5206\u8fa8\u51fa\u7ec6\u80de\u7684\u5f02\u8d28\u6027\u3002\u8fd9\u662f\u8fc7\u53bbRNA-seq\u6240\u65e0\u6cd5\u4f01\u53ca\u7684\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.75\" data-s=\"300,640\" data-type=\"png\" data-w=\"412\" style=\"height: auto !important;\" 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20px;\"><strong>\u5355\u7ec6\u80de\u5efa\u5e93\u6d4b\u5e8f\u6d41\u7a0b<\/strong><\/span><\/em><\/span><\/h1>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.34759358288770054\" data-s=\"300,640\" data-type=\"png\" data-w=\"561\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/3_8H2Zb4PbPnaN1YibAwaGJkT29omPEQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo22AHpmeictHsErQVPbuuuZ9oOicic8H2Zb4PbPnaN1YibAwaGJkT29omPEQ\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"font-size: 14px;\">\u5355\u7ec6\u80de\u6d4b\u5e8f\u7684\u4e09\u4e2a\u57fa\u672c\u6b65\u9aa4\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u5c0f\u679c\u4f1a\u4ece\u6837\u672c\u7684\u5236\u5907\u5f00\u59cb\uff0c\u7136\u540e\u8bb2\u89e310\u00d7genomics\u6587\u5e93\u6784\u5efa\u548c\u6d4b\u5e8f\u7684\u539f\u7406\u53ca\u5176\u4f18\u52bf\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" data-type=\"jpeg\" data-w=\"1080\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/4_IlmBAWe69EMUPMhNEgrv6NXX85lJxg.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwyrj7kbUR3ULG9ZUbLrLkgibzL8qUpQROfe8ebc3gNSnu1dIlmBAWe69EMUPMhNEgrv6NXX85lJxg\/640?wx_fmt=jpeg'\" \/><span style=\"font-family: \u7b49\u7ebf;font-size: 10.5pt;letter-spacing: 0.034em;text-align: justify;\"><\/span><\/p>\n<h1 style=\"text-align: center;\"><strong><span style=\"font-family: \u7b49\u7ebf;font-size: 22pt;\"><o:p><\/o:p><\/span><\/strong><\/h1>\n<h2 style=\"text-align: center;\"><o:p><\/o:p><\/h2>\n<h1 style=\"text-align: center;\"><em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><strong>\u5355\u7ec6\u80de\u6837\u672c\u5236\u5907<\/strong><\/span><\/em><\/h1>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u5355\u7ec6\u80de\u6837\u672c\u7684\u5236\u5907\u6709\u4ee5\u4e0b\u51e0\u79cd\u65b9\u6cd5\u3002<\/span><o:p><\/o:p><\/p>\n<h3><span style=\"font-size: 14px;\">1. Limiting dilution\u6781\u9650\u7a00\u91ca<\/span><\/h3>\n<h3><strong><span style=\"font-family: \u7b49\u7ebf;font-size: 16pt;\"><o:p><\/o:p><\/span><\/strong><\/h3>\n<h1 style=\"text-align: center;\"><strong><span style=\"font-family: \u7b49\u7ebf;font-size: 22pt;\"><o:p><\/o:p><\/span><\/strong><\/h1>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.0227272727272727\" data-s=\"300,640\" data-type=\"png\" data-w=\"176\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/5_PSp1ze7bbSZHcQyu2nx8MlcBicZlJA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo266Ov0zvI08PjtSPcLsw0leuFkPSp1ze7bbSZHcQyu2nx8MlcBicZlJA\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"color: rgb(0, 0, 0);letter-spacing: 0.75pt;font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8fd9\u4e00\u79cd\u5e38\u7528\u7684\u6280\u672f\uff0c\u624b\u52a8\u4f7f\u7528\u79fb\u6db2\u5668\u901a\u8fc7\u7a00\u91ca\u5206\u79bb\u5355\u4e2a\u7ec6\u80de\u3002\u8fd9\u79cd\u65b9\u6cd5\u6548\u7387\u6bd4\u8f83\u4f4e\u3002<\/span><\/p>\n<h3 style=\"margin-bottom: 2.5pt;margin-left: 0pt;text-align: center;line-height: 150%;\"><em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><strong><span style=\"color: rgb(0, 0, 0);\">Micromanipulation\u663e\u5fae\u64cd\u7eb5\u672f<\/span><\/strong><\/span><\/em><\/h3>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.1096774193548387\" data-s=\"300,640\" data-type=\"png\" data-w=\"155\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/6_zTTiacXPh6FHP7bStJic97q45Q2TfQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2vPMlgANsK14Pr9f62plOD22gQmzTTiacXPh6FHP7bStJic97q45Q2TfQ\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"font-size: 14px;font-family: \u5fae\u8f6f\u96c5\u9ed1;color: rgb(0, 0, 0);letter-spacing: 0.75pt;\">\u8be5\u6280\u672f\u975e\u5e38\u7ecf\u5178\uff0c\u66fe\u7528\u4e8e\u4ece\u65e9\u671f\u80da\u80ce\u6216\u672a\u57f9\u517b\u7684\u5fae\u751f\u7269\u4e2d\u63d0\u53d6\u7ec6\u80de\uff0c\u4f7f\u7528\u663e\u5fae\u955c\u5f15\u5bfc\u7684\u6bdb\u7ec6\u7ba1\u79fb\u6db2\uff0c\u6765\u4ece\u60ac\u6d6e\u6db2\u4e2d\u63d0\u53d6\u5355\u7ec6\u80de\u3002\u7136\u800c\uff0c\u8fd9\u4e9b\u65b9\u6cd5\u8017\u65f6\u4e14\u541e\u5410\u91cf\u4f4e\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:\u5fae\u8f6f\u96c5\u9ed1;mso-bidi-font-family:'Times New Roman';color:rgb(0,0,0);letter-spacing:0.7500pt;font-size:11.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<h3 style=\"text-align: center;\"><em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><strong><span style=\"color: rgb(0, 0, 0);\">FACS<\/span><\/strong><\/span><\/em><strong><span style=\"font-family: \u7b49\u7ebf;font-size: 16pt;\"><o:p><\/o:p><\/span><\/strong><\/h3>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7962962962962963\" data-s=\"300,640\" data-type=\"png\" data-w=\"216\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/7_yDjrEqVFhUqf8OM1HzaJYwXDd94M6g.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2XrlYxJRic3I39hLuW2fYzoiaChyDjrEqVFhUqf8OM1HzaJYwXDd94M6g\/640?wx_fmt=png'\" \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">flow-activated cell sorting (FACS)\uff0c\u4f7f\u7528\u6d41\u5f0f\u7ec6\u80de\u4eea\u6765\u5206\u9009\u7ec6\u80de\u3002\u9996\u5148\u7528\u8367\u5149\u5355\u514b\u9686\u6297\u4f53\u6807\u8bb0\u7ec6\u80de\uff0c\u8be5\u6297\u4f53\u8bc6\u522b\u7279\u5b9a\u7684\u8868\u9762\u6807\u8bb0\uff0c\u5e76\u80fd\u591f\u5bf9\u4e0d\u540c\u7684\u7fa4\u4f53\u8fdb\u884c\u5206\u7c7b\u3002\u6216\u8005\uff0c\u5bf9\u4e8e\u672a\u67d3\u8272\u7684\u79cd\u7fa4\uff0c\u8d1f\u9009\u62e9\u4e5f\u662f\u53ef\u80fd\u7684\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u4f46\u662f\u4e0a\u8ff0\u4e09\u79cd\u5c40\u9650\u6027\u6709\uff0c\u5bf9\u8d77\u59cb\u7ec6\u80de\u4f53\u79ef\u7684\u8981\u6c42\uff08\u7ec6\u80de\u6570\u5c11\u4e8e10000\u4f1a\u96be\u4ee5\u5206\u79bb\u7ec6\u80de\uff09\u4ee5\u53ca\u9700\u8981\u5bf9\u611f\u5174\u8da3\u9776\u5411\u86cb\u767d\u8d28\u7684\u6297\u4f53\u3002<\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><br \/><\/span><\/p>\n<h3 style=\"text-align: center;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><strong><span style=\"color: rgb(0, 0, 0);\"> <em>Laser capture microdissection\u6fc0\u5149\u6355\u83b7\u663e\u5fae\u5207\u5272<\/em><\/span><\/strong><\/span><o:p><\/o:p><\/h3>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.3428571428571427\" data-s=\"300,640\" data-type=\"png\" data-w=\"140\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/8_lK5nyQtlO5v0JrcIYlgHQEOCQrmNoQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2hAgyJjGgrQN1mE2RJtelAjMialK5nyQtlO5v0JrcIYlgHQEOCQrmNoQ\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"color: rgb(0, 0, 0);letter-spacing: 0.75pt;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u8be5\u6280\u672f\u5229\u7528\u8ba1\u7b97\u673a\u7cfb\u7edf\u8f85\u52a9\u7684\u6fc0\u5149\u7cfb\u7edf\u4ece\u56fa\u4f53\u6837\u54c1\u4e2d\u5206\u79bb\u7ec6\u80de\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:\u5fae\u8f6f\u96c5\u9ed1;mso-bidi-font-family:'Times New Roman';color:rgb(0,0,0);letter-spacing:0.7500pt;font-size:11.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><o:p><br \/><\/o:p><\/p>\n<h3 style=\"text-align: center;\"><em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><strong><span style=\"color: rgb(0, 0, 0);\">\u4e0e\u6297\u4f53\u7ed3\u5408\u78c1\u94c1\u7b5b\u9009<\/span><\/strong><\/span><\/em><strong><span style=\"font-family: \u7b49\u7ebf;font-size: 16pt;\"><o:p><\/o:p><\/span><\/strong><\/h3>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8483412322274881\" data-s=\"300,640\" data-type=\"png\" data-w=\"211\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/9_1tesibxlTo8jJGU4InjFRhSR5LVf5A.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2gibX5o9gfXGFrQrnEBaRjMEA1o1tesibxlTo8jJGU4InjFRhSR5LVf5A\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"color: rgb(0, 0, 0);letter-spacing: 0.75pt;\">\u8be5\u7cfb\u7edf\u4f7f\u7528\u4e0e\u6297\u4f53\u7ed3\u5408\u7684\u78c1\u94c1\u6765\u68c0\u6d4b\u4e0a\u76ae\u6765\u6e90\u7684CTC\uff08CD45-\u548cEpCAM+\uff09\u3002\u4f8b\u5982\uff0c\u4e3a\u4e86\u5206\u79bb\u7f55\u89c1\u7684\u5faa\u73af\u80bf\u7624\u7ec6\u80de\uff08CTC\uff09\uff0cCellSearch\uff08\u7b2c\u4e00\u4e2a\u7ecf\u8fc7\u4e34\u5e8a\u9a8c\u8bc1\u3001\u7f8e\u56fd\u98df\u54c1\u836f\u54c1\u76d1\u7763\u7ba1\u7406\u5c40\u6279\u51c6\u7684\u6d4b\u8bd5\uff09\u5f00\u53d1\u4e86\u4e00\u79cd\u7cfb\u7edf\u6765\u679a\u4e3e\u60a3\u8005\u8840\u6db2\u6837\u672c\u4e2d\u7684CTC\uff08\u56fe1f\uff09\u3002<\/span><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;color: rgb(0, 0, 0);letter-spacing: 0.75pt;font-size: 11.5pt;\"><o:p><\/o:p><\/span><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><strong><span style=\"color: rgb(0, 0, 0);\"><br \/><\/span><\/strong><\/span><\/p>\n<h3 style=\"text-align: center;\"><em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><strong><span style=\"color: rgb(0, 0, 0);\">Microfluidic technology\u5fae\u6d41\u4f53\u6280\u672f<\/span><\/strong><\/span><\/em><o:p><\/o:p><\/h3>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6517857142857143\" data-s=\"300,640\" data-type=\"png\" data-w=\"224\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/10_L4x18tm4enAbcMQU2uAr7B3oic8a0Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2S26X2bibCEkOhdJZtb3icgicKGyjL4x18tm4enAbcMQU2uAr7B3oic8a0Q\/640?wx_fmt=png'\" \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u7528\u4e8e\u5355\u7ec6\u80de\u5206\u79bb\u7684\u6280\u672f\u7531\u4e8e\u5176\u4f4e\u6837\u54c1\u6d88\u8017\u548c\u4f4e\u5206\u6790\u6210\u672c\u4ee5\u53ca\u80fd\u591f\u5b9e\u73b0\u7cbe\u786e\u7684\u6d41\u4f53\u63a7\u5236\u800c\u5e7f\u53d7\u6b22\u8fce\u3002\u540c\u65f6\uff0c\u8be5\u6280\u672f\u6240\u9700\u7684nL\u5927\u5c0f\u7684\u4f53\u79ef\u5927\u5927\u964d\u4f4e\u4e86\u5916\u90e8\u6c61\u67d3\u7684\u98ce\u9669\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8be5\u6280\u672f\u6700\u521d\u7528\u4e8e\u5c11\u91cf\u7684\u751f\u7269\u5316\u5b66\u5206\u6790\uff0c\u7528\u4e8eDNA\u548c\u86cb\u767d\u8d28\u7684\u5206\u6790\u3002\u73b0\u5728\u5f00\u53d1\u51fa\u4e86\u590d\u6742\u7684\u5fae\u6d41\u9635\u5217\uff0c\u5141\u8bb8\u5bf9\u9600\u95e8\u548c\u5f00\u5173\u8fdb\u884c\u5355\u72ec\u63a7\u5236\uff0c\u4ece\u800c\u63d0\u9ad8\u4e86\u5b83\u4eec\u7684\u53ef\u6269\u5c55\u6027\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8fd1\u5e74\u6765\u5fae\u6d41\u4f53\u6280\u672f\u7684\u5feb\u901f\u53d1\u5c55\u6539\u53d8\u4e86\u57fa\u7840\u79d1\u5b66\u5bb6\u548c\u4e34\u5e8a\u533b\u751f\u7684\u7814\u7a76\u80fd\u529b\u3002\u8be5\u6280\u672f\u53ef\u4ee5\u4ee5\u9ad8\u5ea6\u5e76\u884c\u7684\u65b9\u5f0f\u5bf9\u5355\u4e2a\u7ec6\u80de\u57fa\u56e0\u8868\u8fbe\u8c31\u7684\u91cf\u5316\u3002<\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<h3 style=\"text-align: center;\"><em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><strong><span style=\"color: rgb(0, 0, 0);\">\u51e0\u79cd\u5355\u7ec6\u80de\u5206\u9009\u6280\u672f\u6bd4\u8f83<\/span><\/strong><\/span><\/em><o:p><\/o:p><\/h3>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4602888086642599\" data-s=\"300,640\" data-type=\"png\" data-w=\"554\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/11_Tj8FblUUcicyo9zAtiaFoialOzjMXA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo23gZ9ddYqez0Jkbs8MlCxXial3dTmTj8FblUUcicyo9zAtiaFoialOzjMXA\/640?wx_fmt=png'\" \/><\/p>\n<h1 style=\"text-align: center;\"><em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><strong><span style=\"color: rgb(0, 0, 0);\">10\u00d7 genomics \u6838\u5fc3\u6280\u672f\u539f\u7406<\/span><\/strong><\/span><\/em><\/h1>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">10\u00d7genomics\u5e73\u53f0\u5e94\u7528\u4e86\u5148\u8fdb\u7684\u5fae\u6d41\u4f53\u6280\u672f\u4ee5\u9ad8\u6355\u83b7\u6548\u7387\u63d0\u4f9b\u4e86\u5355\u7ec6\u80deRNA 3\u2032\u7aef\u7684\u9ad8\u901a\u91cf\u5206\u6790\u3002\u56e0\u6b64\uff0c\u8fd9\u79cd\u9ad8\u901a\u91cf\u5904\u7406\u65b9\u6cd5\u80fd\u591f\u5728\u8db3\u591f\u5f02\u8d28\u7684\u751f\u7269\u7a7a\u95f4\u4e2d\u5206\u6790\u7a00\u6709\u7ec6\u80de\u7c7b\u578b\u3002\u76ee\u524d10\u00d7genomics\u5df2\u6210\u4e3a\u5355\u7ec6\u80de\u6d4b\u5e8f\u6700\u53d7\u6b22\u8fce\u7684\u5e73\u53f0\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0b\u9762\u7ee7\u7eed\u7531\u5c0f\u679c\u5e26\u5927\u5bb6\u4e86\u89e3\u4e00\u4e0b\u5b83\u7684\u6838\u5fc3\u6280\u672f\u539f\u7406\u3002<\/span><o:p><\/o:p><\/p>\n<h2 style=\"text-align: center;\"><em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><strong><span style=\"color: rgb(0, 0, 0);\">GEM\uff08gel bead inemulsion\uff09\u51dd\u80f6\u73e0\u4e73\u6db2<\/span><\/strong><\/span><\/em><o:p><\/o:p><\/h2>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5487364620938628\" data-s=\"300,640\" data-type=\"png\" data-w=\"554\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/12_nWSDJib1KRJOFqqkibxFp8Sr0D9OmA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2TaTcypm9NhzlwxoOPicUdiaJwaGmnWSDJib1KRJOFqqkibxFp8Sr0D9OmA\/640?wx_fmt=png'\" \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u6bcf\u4e2abead\u4e0a\u9762\u6709READ1, Barcode, UMI, Poly(dT)VN<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">READ1 \uff1a\u5305\u542b\u6d4b\u5e8f\u7528\u7684\u63a5\u5934adaptor, Index, \u6d4b\u5e8f\u5f15\u7269\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">Barcode\uff0c\u6bcf\u4e2aGEM\u5e26\u6709\u552f\u4e00\u7684Barcod\u6761\u5f62\u7801\uff0c\u7528\u6765\u533a\u5206\u7ec6\u80de\uff0c\u8bc6\u522b\u4e0d\u540c\u7684\u7ec6\u80de\u3002\u901a\u5e38\u670916bp\uff0c\u51714\u768416\u6b21\u65b9\u79cd\u53d8\u5316\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">UMI\uff1a\u7279\u5f02\u7684\u5206\u5b50\u7247\u6bb5\uff0c\u7528\u6765\u6807\u8bb0reads\u3002\u7528\u6765\u89e3\u51b3pcr\u6269\u589e\u5f15\u5165\u7684\u504f\u5dee\uff0c \u504f\u5dee\u5bf9\u5355\u7ec6\u80de\u5f71\u54cd\u5f88\u5927\u3002\u901a\u5e38\u6bcf\u4e2abeads\u62e5\u6709\u4e00\u7ec4\u4e0d\u540c\u7684UMI\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">Poly(dT)VN\uff1a\u4e0emRNA\u7684PloyA\u5c3e\u4e92\u8865\uff0c\u7528\u6765\u6355\u83b7\u7ec6\u80de\u5185\uff0c\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.40974729241877256\" data-s=\"300,640\" data-type=\"png\" data-w=\"554\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/13_c6HXxpeAtXyicr3Lup5MBowUtWq9uw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2CHPtFbbPWBj4zzZrGic5t95Uhqic6HXxpeAtXyicr3Lup5MBowUtWq9uw\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">CB\u5373cell barcode, \u4e0d\u540c\u7248\u672cCB\u4e0eUMI\u7684\u957f\u5ea6\u53d8\u5316\u3002<\/span><o:p><\/o:p><\/p>\n<h1 style=\"text-align: center;\"><em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><strong>1<\/strong><strong>0<\/strong><strong>\u00d7genomics\u5355\u7ec6\u80de\u5206\u9009<\/strong><\/span><\/em><strong><span style=\"font-family: \u7b49\u7ebf;font-size: 22pt;\"><o:p><\/o:p><\/span><\/strong><\/h1>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4584837545126354\" data-s=\"300,640\" data-type=\"png\" data-w=\"554\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/14_QXIrBLaDPjGpRLrKTkMmHdibxqkObQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2JPdP15p72CVakHjXrRAYAC5WLQXIrBLaDPjGpRLrKTkMmHdibxqkObQ\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8be5\u5e73\u53f0\u7684\u5355\u7ec6\u80de\u5206\u79bb\u6280\u672f\u662f\u57fa\u4e8e\u5fae\u6ef4\u7684\u5fae\u6d41\u4f53\u3002\u5b83\u5141\u8bb8\u6c34\u6ef4\u5728\u8fde\u7eed\u6cb9\u76f8\u4e2d\u5355\u5206\u6563\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">GEM\u4f1a\u4ece\u5de6\u5f80\u53f3\u6d41\u52a8\uff0c\u5728\u7b2c\u4e00\u4e2a\u5341\u5b57\u8def\u53e3\uff0c\u6837\u672c\u7ec6\u80de\u4f1a\u8fdb\u5165\u548c\u51dd\u80f6\u73e0\u7ed3\u5408\uff0c\u5728\u7b2c\u4e8c\u4e2a\u5341\u5b57\u8def\u53e3\u4f1a\u88ab\u6cb9\u6ef4\u5305\u88f9\uff0c\u5b9e\u73b0\u5355\u7ec6\u80de\u7684\u6355\u83b7\u3002\u4e0e\u6807\u51c6\u5fae\u6d41\u4f53\u5ba4\u76f8\u6bd4\uff0c\u8be5\u7cfb\u7edf\u6240\u9700\u7684\u7ec6\u80de\u4f53\u79ef\u66f4\u4f4e\uff0c\u80fd\u591f\u4ee5\u66f4\u4f4e\u7684\u6210\u672c\u64cd\u4f5c\u548c\u7b5b\u9009\u6570\u5343\u81f3\u6570\u767e\u4e07\u4e2a\u7ec6\u80de\u3002<\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<h1 style=\"text-align: center;\"><em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><strong>\u8d85\u9ad8\u7684\u7ec6\u80de\u901a\u91cf<\/strong><\/span><\/em><\/h1>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5018050541516246\" data-s=\"300,640\" data-type=\"png\" data-w=\"554\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/15_WIwdMUt4HNAhPmQFRwLV3RCp3jiahw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2vNDLg3s864XjNozUrJ8RtsibrGWIwdMUt4HNAhPmQFRwLV3RCp3jiahw\/640?wx_fmt=png'\" \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u2460\u5b54\u52a0\u5165\u7ec6\u80de\u6837\u672c\uff0c\u2461\u5b54\u52a0\u5165\u51dd\u80f6\u73e0\uff0c\u2462\u52a0\u5165\u6cb9\u6ef4<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u6bcf\u4e2a\u5fae\u6d41\u5b54\u6709\u516b\u6761line\uff0c\u6bcf\u4e2aline\uff0c10000\u4e2a\u7ec6\u80de\uff0c\u6bcf\u6b21 \u901a\u91cf\u53ef\u8fbe8\u4e07\u3002<\/span><\/p>\n<h1 style=\"text-align: center;\"><em><span style=\"font-size: 20px;\"><strong><span style=\"font-family: \u7b49\u7ebf;\">1<\/span><\/strong><strong><span style=\"font-family: \u7b49\u7ebf;\">0<\/span><\/strong><strong><span style=\"font-family: \u7b49\u7ebf;\">\u00d7genomics\u5efa\u5e93\u6d4b\u5e8f\u6d41\u7a0b<\/span><\/strong><\/span><\/em><strong><span style=\"font-family: \u7b49\u7ebf;font-size: 22pt;\"><o:p><\/o:p><\/span><\/strong><\/h1>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.38267148014440433\" data-s=\"300,640\" data-type=\"png\" data-w=\"554\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/16_I6mZ6CFenoVDO9HZiapCKkOpP07lGA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2icHfTe8Vk0FtOObS6LKY25VRia7I6mZ6CFenoVDO9HZiapCKkOpP07lGA\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">1.\u6cb9\u6ef4\u51dd\u80f6\u73e0\u6355\u83b7\u5355\u7ec6\u80de\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">2.\u5355\u7ec6\u80de\u88c2\u89e3\uff0c\u51dd\u80f6\u73e0\u4e0a\u7684PolyT\u4f1a\u6355\u83b7mRNA\u7684PloyA\u5c3e<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">3.\u6d4b\u5e8f<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">4.\u6309\u7167barcode\u5206\u7c7b\u7ec6\u80de<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">5.\u6309\u7167UMI\u53bb\u91cd\u590dreads<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4566787003610108\" data-s=\"300,640\" data-type=\"png\" data-w=\"554\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/17_l62xrGDPPicahp8jtWvwja0s63AibA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2b82z4jg7aRAz0Aiaeht1uGrx5E0l62xrGDPPicahp8jtWvwja0s63AibA\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<h2 style=\"text-align: center;\"><span style=\"font-size: 20px;\"><em><strong><span style=\"font-family: "\u7b49\u7ebf Light";\">\u5efa\u5e93<\/span><\/strong><\/em><em><strong><span style=\"font-family: "\u7b49\u7ebf Light";font-size: 16pt;\"><o:p><\/o:p><\/span><\/strong><\/em><\/span><\/h2>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7617328519855595\" data-s=\"300,640\" data-type=\"png\" data-w=\"554\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/18_FvG6OicL0bwasv9eHWwgkMgNqLPCBA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2MB5kIHx7icibEP5krsHKyPvGSDUFvG6OicL0bwasv9eHWwgkMgNqLPCBA\/640?wx_fmt=png'\" \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">10\u00d7 genomics\u6d4b\u5e8f\u4e0eillumina\u5951\u5408\u5ea6\u5f88\u9ad8\uff0c\u4e0eillumina\u5e73\u53f0\u5efa\u5e93\u65b9\u6cd5\u5dee\u4e0d\u591a<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">1.PolyT\u6355\u83b7mRNA<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">2.mRna\u6253\u65ad<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">3.\u7b2c\u4e00\u94fe\u5408\u6210<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">4.\u7b2c\u4e8c\u94fe\u5408\u6210<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">5.3\u2019\u4e0e5\u2018\u7aef\u4fee\u9970<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">6.\u8fde\u63a5adapter<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">7.PCR\u6269\u589e<\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<h2 style=\"text-align: center;\"><span style=\"font-size: 20px;\"><em><strong><span style=\"font-family: "\u7b49\u7ebf Light";\">\u6d4b\u5e8f<\/span><\/strong><\/em><\/span><strong><span style=\"font-family: "\u7b49\u7ebf Light";font-size: 16pt;\"><o:p><\/o:p><\/span><\/strong><\/h2>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2563176895306859\" data-s=\"300,640\" data-type=\"png\" data-w=\"554\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/19_8nwicI9lbWXSNImljMZp0v2OtVwVKw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2QzfLz4J4ZLAJzuY8AW7O9qficf8nwicI9lbWXSNImljMZp0v2OtVwVKw\/640?wx_fmt=png'\" \/><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">Read1:P5\u63a5\u5934\u4e0eilumina\u4e00\u6837\uff0cRead1\u5305\u542b\u63a5\u5934adaptor, Index,\u6d4b\u5e8f\u5f15\u7269\u7b49\u4ece\u7eff\u8272\u7684\u90e8\u5206\u5f00\u59cb\u6d4b\u6d4b\u5230UMI. \u6700\u540e\u6d4b16+12=28bp.<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">Read2:\u4ece\u5e95\u90e8\u94feTruSeq\u5f00\u59cb\u6d4b\uff0c\u6d4b91bp\uff0c<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">read2\u6d4b\u5230\u7684\u662fmRNA\u5e8f\u5217\u4fe1\u606f\uff0cread1\u6d4b\u7684\u662fbarcode,UMI<\/span><\/p>\n<h2 style=\"text-align: center;\"><span style=\"font-size: 20px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><em><strong>1<\/strong><strong>0<\/strong><strong>\u00d7genomic\u6d4b\u5e8f\u7684\u7ed3\u679c\u662f3\u2018\u7aef\u6570\u636e<\/strong><\/em><\/span><\/h2>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7545126353790613\" data-s=\"300,640\" data-type=\"png\" data-w=\"554\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-06_3\u5206\u949f\u8bfb\u61c2\u6700\u53d7\u6b22\u8fce\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f010\u00d7genomics\u7ec6\u80de\u5206\u9009\uff0c\u5efa\u5e93\u53ca\u6d4b\u5e8f\/20_WABzootUVJVchKB263MWXKRBozlvVA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy7v8UlSQWC5IAA9KDZVCo2llptPymSZ4MJIhgzVl8FpSLNWABzootUVJVchKB263MWXKRBozlvVA\/640?wx_fmt=png'\" \/><\/p>\n<h1><\/h1>\n<h1><span style=\"font-size: 14px;\">\u4f18\u52bf\uff1a<\/span><o:p><\/o:p><\/h1>\n<p><span style=\"font-size: 14px;\">1.\u771f\u6b63\u5355\u7ec6\u80de\u6d4b\u5e8f<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">2.\u8d85\u9ad8\u7ec6\u80de\u901a\u91cf<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">3.\u7ec6\u80de\u6355\u83b7\u6548\u7387\u9ad8<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">4.\u7ec6\u80de\u53ef\u9002\u6027\u9ad8<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">5.\u591a\u6001\u7387\u4f4e<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">6.\u65f6\u95f4\u5468\u671f\u77ed<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">7.\u6027\u4ef7\u6bd4\u9ad8<\/span><\/p>\n<h1 style=\"text-align: center;\"><span style=\"font-size: 20px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><em><strong>\u603b\u7ed3\uff1a<\/strong><\/em><\/span><strong><span style=\"font-family: \u7b49\u7ebf;font-size: 22pt;\"><o:p><\/o:p><\/span><\/strong><\/h1>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">1\u3001\u6587\u5e93\u6784\u5efa\u65f6\u6709\u5f88\u591a\u79cd\u7c7b\u7684GEMs<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">2\u3001\u901a\u8fc7\u5408\u7406\u63a7\u5236\u6bcf\u6b21\u8f93\u5165\u7684\u7ec6\u80de\u6570\uff0c\u6765\u6700\u7ec8\u63a7\u5236\u68c0\u6d4b\u7684\u7ec6\u80de\u6570;\u76ee\u524d\u5e73\u5c40\u53ef\u4ee5\u68c0\u6d4b3000-8000\u4e2a\u7ec6\u80de;<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">3\u3001\u4e00\u822c\u6765\u8bf4\uff0c\u7ea6\u670965%\u7684GEM\u572810X\u82af\u7247\u7684\u7ba1\u9053\u4e2d\u53ef\u4ee5\u6210\u529f\u6355\u83b7\u7ec6\u80de\uff0c\u4f46\u4e5f\u670935%\u6ca1\u6709\u6355\u83b7\u7ec6\u80de\uff0c\u79f0\u4e3a\u7a7a\u8f7d;<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">4\u3001\u7a7a\u8f7d\u7684GEM\u5e76\u975e\u6ca1\u6709\u4efb\u4f55\u7ed3\u679c\u3002\u7531\u4e8e\u5355\u7ec6\u80de\u60ac\u6db2\u4e2d\u5b58\u5728\u7ec6\u80de\u7834\u88c2\u4ea7\u751f\u7684\u6e38\u79bbRNA\u56e0\u6b64\u7a7a\u8f7dGEM\u4e2d\u4e5f\u4f1a\u6269\u589e\u5f97\u5230\u5c11\u91cfRNA\u7684\u4fe1\u606f\uff0c\u4f46\u57fa\u56e0\u6570\u4f1a\u5f88\u5c11<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">5\u3001\u6709\u90e8\u5206GEM\u4e2d\u4f1a\u5305\u542b\u4e24\u4e2a\u7ec6\u80de\u79f0\u4e3adoublets\uff0c\u8d85\u8fc7\u4e24\u4e2a\u79f0\u4e3amultiplets\u30026\u3001\u6700\u7ec8\u8981\u5c06\u7a7a\u8f7d\u6216\u8005\u8fc7\u8f7d\u7684\u975e\u5355\u7ec6\u80de\u8fc7\u6ee4\u6389<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">6\u3001\u548cilummina\u5e73\u53f0\u5173\u7cfb\u7d27\u5bc6\uff0c\u53ef\u4ee5\u653610\u00d7genomics\u8bbe\u5907\u7528\u6765\u6355\u83b7\u5355\u7ec6\u80de\uff0c\u6d4b\u5e8f\u5728ilumina\u5e73\u53f0\u6d4b\u5e8f\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">7\u3001\u7ec6\u80de\u7834\u788e\u540e\uff0c\u6837\u672c\u6df7\u5408\u5728\u4e00\u8d77\u6d4b\u5e8f\uff0c\u901a\u8fc7barcode\u6765\u8bc6\u522b\u7ec6\u80de\uff0c\u6709\u591a\u5c11barcode\u5c31\u6709\u591a\u5c11\u7ec6\u80de\u3002\u6bcf\u4e2a\u7ec6\u80de\u91cc\u9762\u6709\u591a\u5c11\u6761UMI\u5c31\u6d4b\u4e86\u591a\u5c11\u6761reads\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, 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