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powered-by=\"xiumi.us\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 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src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_GBhsibRAgNiaQsH4ichdP9ibEhHMFA-83.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYngnlT6jiavu08Bvp2iblzGf3IkoBD9NGBhsibRAgNiaQsH4ichdP9ibEhHMFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"400\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section style=\"display: contents;\" powered-by=\"xiumi.us\">\n<section style=\"display: contents;\">\n<section style=\"display: contents;\">\n<section style=\"height: 0px;overflow: hidden;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;caret-color: red;\"> <\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5fae\u5c0fRNA\u7684\u8868\u8fbe\u6570\u636e\u4e0e\u60a3\u8005\u7684\u751f\u5b58\u6570\u636e\u8fdb\u884c\u5206\u6790\uff0c\u4ee5\u5bfb\u627e\u4e0e\u60a3\u8005\u751f\u5b58\u76f8\u5173\u7684miRNA\u3002\u751f\u5b58\u5206\u6790\u548c\u7ed8\u5236\u751f\u5b58\u66f2\u7ebf\uff0c\u627e\u51fa\u4e0e\u60a3\u8005\u751f\u5b58\u76f8\u5173\u7684miRNA\u3002\u8fd9\u5bf9\u4e8e\u4e86\u89e3miRNA\u5728\u764c\u75c7\u7b49\u75be\u75c5\u9884\u540e\u4e2d\u7684\u4f5c\u7528\u4ee5\u53ca\u5bfb\u627e\u6f5c\u5728\u7684\u751f\u7269\u6807\u5fd7\u7269\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002\u751f\u5b58\u5206\u6790\u662f\u4e00\u79cd\u5e38\u7528\u7684\u7edf\u8ba1\u5206\u6790\u65b9\u6cd5\uff0c\u53ef\u7528\u4e8e\u8bc4\u4f30\u4e0d\u540c\u57fa\u56e0\u6216\u751f\u7269\u5206\u5b50\u5728\u60a3\u8005\u9884\u540e\u4e2d\u7684\u6f5c\u5728\u5f71\u54cd\u3002\u901a\u8fc7\u8be5\u811a\u672c\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u5feb\u901f\u800c\u5168\u9762\u5730\u5bf9\u5927\u89c4\u6a21miRNA\u8868\u8fbe\u6570\u636e\u8fdb\u884c\u751f\u5b58\u76f8\u5173\u5206\u6790\uff0c\u4ece\u800c\u8f85\u52a9\u764c\u75c7\u7b49\u75be\u75c5\u7684\u7814\u7a76\u548c\u8bca\u65ad\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong><span style=\"font-size: 12pt;\">\u4ee3\u7801\u4e2d\u5bf9<\/span><\/strong><strong><span style=\"font-size: 12pt;\">\u9009\u5b9a\u7684\u76f8\u5173<\/span><\/strong><strong><span style=\"font-size: 12pt;\">miRNA\u7684\u8868\u8fbe\u91cf\u8fdb\u884c<\/span><\/strong><strong><span style=\"font-size: 12pt;\">\u751f\u5b58\u5206\u6790<\/span><\/strong><strong><span style=\"font-size: 12pt;\">\u3002<\/span><\/strong><strong><span style=\"font-size: 12pt;\">\u4e0b\u9762\u662f\u8981\u7814\u7a76\u7684\u591a\u4e2amirna\u7684\u6837\u672c\u8868\u8fbe\u91cf\uff0c\u4ee5\u53ca\u4e0b\u8f7d\u7684STAD\u764c\u75c7\u7684\u751f\u5b58\u65f6\u95f4\u548c\u72b6\u6001\u3002<\/span><\/strong><\/span><strong><span style=\"font-size: 12pt;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><\/span><\/strong><\/p>\n<p><strong><span style=\"font-size: 12pt;font-family: \u5b8b\u4f53;\"><br \/><\/span><\/strong><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2745664739884393\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_MDic1Xkt7E6HGtHpovic26CviciacA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiafuKNiajYIicLGASWOa3Yu6WxLsnwlWMDic1Xkt7E6HGtHpovic26CviciacA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"692\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9875259875259875\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_QNwwIymk7FtWRhQqc05gtibf59ogcw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiaKYYVfuKnNgQFG5oT2x9Y0mRfbQNwwIymk7FtWRhQqc05gtibf59ogcw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"481\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: center;white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span><span class=\"code-snippet__keyword\">if<\/span> (!requireNamespace(<span class=\"code-snippet__string\">\"BiocManager\"<\/span>, quietly = TRUE))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span> install.packages(<span class=\"code-snippet__string\">\"BiocManager\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span>BiocManager::install(<span class=\"code-snippet__string\">\"limma\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u68c0\u67e5\u662f\u5426\u5df2\u5b89\u88c5”BiocManager”\u5305\uff0c\u5982\u679c\u6ca1\u6709\u5b89\u88c5\uff0c\u5219\u901a\u8fc7install.packages\u51fd\u6570\u5b89\u88c5\u5b83\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">“BiocManager”\u5305\u7528\u4e8e\u7ba1\u7406\u548c\u5b89\u88c5\u751f\u7269\u4fe1\u606f\u5b66\u76f8\u5173\u7684\u5176\u4ed6\u5305\u3002<\/span><\/p>\n<p style=\"text-align: center;white-space: normal;font-size: 16px;\"><o:p><\/o:p><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span>install.packages(<span class=\"code-snippet__string\">'survival'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span>install.packages(<span class=\"code-snippet__string\">\"survminer\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5b89\u88c5”survminer”\u5305\uff0c\u5b83\u662f\u4e00\u4e2a\u7528\u4e8e\u751f\u5b58\u6570\u636e\u53ef\u89c6\u5316\u548c\u7edf\u8ba1\u5206\u6790\u7684\u5305\u3002<span style=\"font-size: 14px;font-family: \u5b8b\u4f53;\"><\/span><\/span><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code><span class=\"code-snippet_outer\">library(limma)<\/span><\/code><code><span class=\"code-snippet_outer\">library(survival)<\/span><\/code><code><span class=\"code-snippet_outer\">library(survminer)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">expFile=<span class=\"code-snippet__string\">\"cor.MirnaExp.txt\"<\/span> <span class=\"code-snippet__meta\">#\u5171\u8868\u8fbemiRNA\u7684\u8868\u8fbe\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cliFile=<span class=\"code-snippet__string\">\"TCGA-STAD.survival.tsv\"<\/span> <span class=\"code-snippet__meta\">#\u751f\u5b58\u6570\u636e\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u6307\u5b9a\u4e86miRNA\u8868\u8fbe\u6570\u636e\u6587\u4ef6\u548c\u751f\u5b58\u6570\u636e\u6587\u4ef6\u5e76\u5c06\u5b83\u4eec\u5206\u522b\u8d4b\u503c\u7ed9\u53d8\u91cf<span class=\"code-snippet__meta-string\">\"expFile\"<\/span>\u548c<span class=\"code-snippet__meta-string\">\"cliFile\"<\/span><\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8bfb\u53d6\u8868\u8fbe\u6587\u4ef6\uff0c\u5e76\u5bf9\u8f93\u5165\u6587\u4ef6\u6574\u7406<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=read.table(expFile, header=T, sep=<span class=\"code-snippet__string\">\"t\"<\/span>, check.names=F, row.names=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u4f7f\u7528read.table\u51fd\u6570\u8bfb\u53d6\u540d\u4e3a<span class=\"code-snippet__meta-string\">\"cor.MirnaExp.txt\"<\/span>\u7684miRNA\u8868\u8fbe\u6587\u4ef6\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5220\u6389\u6b63\u5e38\u6837\u54c1<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(colnames(data),<span class=\"code-snippet__string\">\"\\-\"<\/span>), <span class=\"code-snippet__string\">\"[\"<\/span>, <span class=\"code-snippet__number\">4<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(group,<span class=\"code-snippet__string\">\"\"<\/span>), <span class=\"code-snippet__string\">\"[\"<\/span>, <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">group=gsub(<span class=\"code-snippet__string\">\"2\"<\/span>, <span class=\"code-snippet__string\">\"1\"<\/span>, group)<\/span><\/code><code><span class=\"code-snippet_outer\">data=t(data[,group==<span class=\"code-snippet__number\">0<\/span>,drop=F])<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(data)=gsub(<span class=\"code-snippet__string\">\"(.*?)\\-(.*?)\\-(.*?)\\-(.*?)\\-.*\"<\/span>, <span class=\"code-snippet__string\">\"\\1\\-\\2\\-\\3-\\4\"<\/span>, rownames(data))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5bf9\u8bfb\u53d6\u7684miRNA\u8868\u8fbe\u6570\u636e\u8fdb\u884c\u9884\u5904\u7406\u3002\u9996\u5148\uff0c\u4ece\u6837\u672c\u540d\u4e2d\u63d0\u53d6\u6570\u5b57\u90e8\u5206\u4ee5\u4fbf\u533a\u5206\u6b63\u5e38\u6837\u672c#\u548c\u80bf\u7624\u6837\u672c\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u7136\u540e\uff0c\u6839\u636e<span class=\"code-snippet__meta-string\">\"group\"<\/span>\u53d8\u91cf\u4e2d\u7684\u503c\uff0c\u5c06\u6b63\u5e38\u6837\u672c\u5bf9\u5e94\u7684\u5217\u4ece\u6570\u636e\u4e2d\u5220\u9664\u3002\u6700\u540e\uff0c\u6839\u636e\u6b63\u5219\u8868\u8fbe#\u5f0f\uff0c\u5c06\u884c\u540d\u7684\u6837\u672c\u7f16\u53f7\u8fdb\u884c\u8c03\u6574\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8bfb\u53d6\u751f\u5b58\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cli=read.table(cliFile, header=T, sep=<span class=\"code-snippet__string\">\"t\"<\/span>, check.names=F, row.names=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">cli=cli[,c(<span class=\"code-snippet__number\">3<\/span>,<span class=\"code-snippet__number\">1<\/span>)]<\/span><\/code><code><span class=\"code-snippet_outer\">cli=na.omit(cli)<\/span><\/code><code><span class=\"code-snippet_outer\">colnames(cli)=c(<span class=\"code-snippet__string\">\"futime\"<\/span>,<span class=\"code-snippet__string\">\"fustat\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">cli$futime=cli$futime\/<span class=\"code-snippet__number\">365<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8bfb\u53d6\u540d\u4e3a<span class=\"code-snippet__meta-string\">\"TCGA-STAD.survival.tsv\"<\/span>\u7684\u751f\u5b58\u6570\u636e\u6587\u4ef6\u3002\u6587\u4ef6\u4ee5\u5236\u8868\u7b26\u5206\u9694\uff0c\u7b2c\u4e00\u884c\u662f\u5217\u540d\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u6240\u4ee5header=T\u8868\u793a\u7b2c\u4e00\u884c\u662f\u5217\u540d\u3002sep=<span class=\"code-snippet__meta-string\">\"t\"<\/span>\u8868\u793a\u6587\u4ef6\u4e2d\u7684\u5b57\u6bb5\u662f\u7528\u5236\u8868\u7b26\u5206\u9694\u7684\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">check.names=F\u8868\u793a\u4e0d\u68c0\u67e5\u5217\u540d\u7684\u5408\u6cd5\u6027\u3002row.names=<span class=\"code-snippet__number\">1<\/span>\u8868\u793a\u4f7f\u7528\u7b2c\u4e00\u5217\u4f5c\u4e3a\u884c\u540d\u3002<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u751f\u5b58\u6570\u636e\u6587\u4ef6\u4e2d\u7684\u5217\u610f\u4e49\u5206\u522b\u662f\uff1a\u7b2c1\u5217\u662f\u6837\u672cID\uff0c\u7b2c3\u5217\u662f\u751f\u5b58\u65f6\u95f4\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u7b2c2\u5217\u662f\u751f\u5b58\u72b6\u6001\uff080\u8868\u793a\u751f\u5b58\uff0c1\u8868\u793a\u6b7b\u4ea1\uff09\u3002\u63a5\u4e0b\u6765\u7684\u4ee3\u7801\u5c06\u8bfb\u53d6\u7684\u751f\u5b58\u6570\u636e\u8fdb\u884c\u5904\u7406\uff1a<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u63d0\u53d6\u7b2c1\u5217\u548c\u7b2c3\u5217\uff08\u751f\u5b58\u65f6\u95f4\u548c\u751f\u5b58\u72b6\u6001\uff09\uff0c\u5220\u9664\u53ef\u80fd\u5305\u542b\u7f3a\u5931\u503c\u7684\u884c\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5e76\u5c06\u5217\u540d\u6539\u4e3a<span class=\"code-snippet__meta-string\">\"futime\"<\/span>\u548c<span class=\"code-snippet__meta-string\">\"fustat\"<\/span>\u3002\u6700\u540e\uff0c\u5c06\u751f\u5b58\u65f6\u95f4\u8f6c\u6362\u4e3a\u5e74\u4e3a\u5355\u4f4d\uff08\u9664\u4ee5365\uff09\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u6570\u636e\u5408\u5e76<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sameSample=intersect(row.names(data), row.names(cli))<\/span><\/code><code><span class=\"code-snippet_outer\">data=data[sameSample,,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">cli=cli[sameSample,,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">rt=cbind(cli, data)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8bfb\u53d6\u540d\u4e3a<span class=\"code-snippet__meta-string\">\"TCGA-STAD.survival.tsv\"<\/span>\u7684\u751f\u5b58\u6570\u636e\u6587\u4ef6\u3002\u6587\u4ef6\u4ee5\u5236\u8868\u7b26\u5206\u9694\uff0c\u7b2c\u4e00\u884c\u662f\u5217\u540d\uff0c\u6240#\u4ee5header=T\u8868\u793a\u7b2c\u4e00\u884c\u662f\u5217\u540d\u3002sep=<span class=\"code-snippet__meta-string\">\"t\"<\/span>\u8868\u793a\u6587\u4ef6\u4e2d\u7684\u5b57\u6bb5\u662f\u7528\u5236\u8868\u7b26\u5206\u9694\u7684\u3002check.names=F\u8868\u793a\u4e0d\u68c0\u67e5\u5217\u540d\u7684\u5408\u6cd5\u6027\u3002row.names=1\u8868\u793a\u4f7f\u7528\u7b2c\u4e00\u5217\u4f5c\u4e3a\u884c\u540d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u751f\u5b58\u6570\u636e\u6587\u4ef6\u4e2d\u7684\u5217\u610f\u4e49\u5206\u522b\u662f\uff1a\u7b2c1\u5217\u662f\u6837\u672cID\uff0c\u7b2c3\u5217\u662f\u751f\u5b58\u65f6\u95f4\uff0c\u7b2c2\u5217\u662f\u751f\u5b58\u72b6#\u6001\uff080\u8868\u793a\u751f\u5b58\uff0c1\u8868\u793a\u6b7b\u4ea1\uff09\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u63a5\u4e0b\u6765\u7684\u4ee3\u7801\u5c06\u8bfb\u53d6\u7684\u751f\u5b58\u6570\u636e\u8fdb\u884c\u5904\u7406\uff1a\u63d0\u53d6\u7b2c1\u5217\u548c\u7b2c3\u5217\uff08\u751f\u5b58\u65f6\u95f4\u548c\u751f\u5b58\u72b6\u6001\uff09\uff0c#\u5220\u9664\u53ef\u80fd\u5305\u542b\u7f3a\u5931\u503c\u7684\u884c\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5e76\u5c06\u5217\u540d\u6539\u4e3a<span class=\"code-snippet__meta-string\">\"futime\"<\/span>\u548c<span class=\"code-snippet__meta-string\">\"fustat\"<\/span>\u3002\u6700\u540e\uff0c\u5c06\u751f\u5b58\u65f6\u95f4\u8f6c\u6362\u4e3a\u5e74\u4e3a\u5355\u4f4d\uff08\u9664\u4ee5365\uff09\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5bf9miRNA\u8fdb\u884c\u5faa\u73af\uff0c\u627e\u51fa\u9884\u540e\u76f8\u5173\u7684miRNA<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">outTab=data.frame()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u521b\u5efa\u4e00\u4e2a\u7a7a\u7684\u6570\u636e\u6846<span class=\"code-snippet__meta-string\">\"outTab\"<\/span>\u6765\u4fdd\u5b58\u6bcf\u4e2amiRNA\u7684\u5206\u6790\u7ed3\u679c\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(gene <span class=\"code-snippet__keyword\">in<\/span> colnames(rt)[<span class=\"code-snippet__number\">3<\/span>:ncol(rt)]){<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\">#\u63d0\u53d6miRNA\u4fe1\u606f<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">if<\/span>(sd(rt[,gene])<<span class=\"code-snippet__number\">0.1<\/span>){next}<\/span><\/code><code><span class=\"code-snippet_outer\"> data=rt[,c(<span class=\"code-snippet__string\">\"futime\"<\/span>, <span class=\"code-snippet__string\">\"fustat\"<\/span>, gene)]<\/span><\/code><code><span class=\"code-snippet_outer\"> colnames(data)=c(<span class=\"code-snippet__string\">\"futime\"<\/span>, <span class=\"code-snippet__string\">\"fustat\"<\/span>, <span class=\"code-snippet__string\">\"gene\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\">#\u83b7\u53d6\u6700\u4f18cutoff,\u5e76\u5bf9\u6837\u54c1\u8fdb\u884c\u5206\u7ec4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> res.cut=surv_cutpoint(data, time = <span class=\"code-snippet__string\">\"futime\"<\/span>, event = <span class=\"code-snippet__string\">\"fustat\"<\/span>, variables =c(<span class=\"code-snippet__string\">\"gene\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"> res.cat=surv_categorize(res.cut)<\/span><\/code><code><span class=\"code-snippet_outer\"> fit=survfit(Surv(futime, fustat) ~gene, data = res.cat)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\">#\u6bd4\u8f83\u9ad8\u4f4e\u8868\u8fbe\u7ec4\u751f\u5b58\u5dee\u5f02<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> diff=survdiff(Surv(futime, fustat) ~gene, data =res.cat)<\/span><\/code><code><span class=\"code-snippet_outer\"> pValue=<span class=\"code-snippet__number\">1<\/span>-pchisq(diff$chisq, df=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> outVector=cbind(gene, res.cut$cutpoint[<span class=\"code-snippet__number\">1<\/span>], pValue)<\/span><\/code><code><span class=\"code-snippet_outer\"> outTab=rbind(outTab,outVector)<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">if<\/span>(pValue<<span class=\"code-snippet__number\">0.001<\/span>){<\/span><\/code><code><span class=\"code-snippet_outer\"> pValue=<span class=\"code-snippet__string\">\"p<0.001\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> }<span class=\"code-snippet__keyword\">else<\/span>{<\/span><\/code><code><span class=\"code-snippet_outer\"> pValue=paste0(<span class=\"code-snippet__string\">\"p=\"<\/span>,sprintf(<span class=\"code-snippet__string\">\"%.03f\"<\/span>,pValue))<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\">#\u7ed8\u5236\u751f\u5b58\u66f2\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> surPlot=ggsurvplot(fit,<\/span><\/code><code><span class=\"code-snippet_outer\"> data=res.cat,<\/span><\/code><code><span class=\"code-snippet_outer\"> pval=pValue,<\/span><\/code><code><span class=\"code-snippet_outer\"> pval.size=<span class=\"code-snippet__number\">6<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.title=gene,<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.labs=c(<span class=\"code-snippet__string\">\"high\"<\/span>,<span class=\"code-snippet__string\">\"low\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab=<span class=\"code-snippet__string\">\"Time(years)\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> ylab=<span class=\"code-snippet__string\">\"Overall survival\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> palette=c(<span class=\"code-snippet__string\">\"red\"<\/span>, <span class=\"code-snippet__string\">\"blue\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">break<\/span>.time.by=<span class=\"code-snippet__number\">1<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> conf.int=T,<\/span><\/code><code><span class=\"code-snippet_outer\"> risk.table=F,<\/span><\/code><code><span class=\"code-snippet_outer\"> risk.table.title=<span class=\"code-snippet__string\">\"\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> risk.table.height=<span class=\"code-snippet__number\">.25<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\">#\u8f93\u51fa\u56fe\u5f62<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> pdf(file=paste0(<span class=\"code-snippet__string\">\"sur.\"<\/span>,gene,<span class=\"code-snippet__string\">\".pdf\"<\/span>),onefile = <span class=\"code-snippet__literal\">FALSE<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> width = <span class=\"code-snippet__number\">5.5<\/span>, <span class=\"code-snippet__meta\">#\u56fe\u7247\u7684\u5bbd\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> height =<span class=\"code-snippet__number\">5<\/span>) <span class=\"code-snippet__meta\">#\u56fe\u7247\u7684\u9ad8\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> print(surPlot)<\/span><\/code><code><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5faa\u73af\u5bf9\u6bcf\u4e2amiRNA\u8fdb\u884c\u751f\u5b58\u5206\u6790\uff0c\u5e76\u7ed8\u5236\u751f\u5b58\u66f2\u7ebf\u3002\u4e3b\u8981\u6b65\u9aa4\u5982\u4e0b\uff1a<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\"># \u4f7f\u7528<span class=\"code-snippet__meta-string\">\"for\"<\/span>\u5faa\u73af\u904d\u5386\u4ece\u7b2c3\u5217\u5f00\u59cb\u5230\u6700\u540e\u4e00\u5217\u7684\u6240\u6709miRNA\u7684\u5217\u540d\u3002\u5728\u8fd9\u91cc\uff0c\u6bcf\u4e2amiRNA<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u7684\u8868\u8fbe\u6570\u636e\u548c\u751f\u5b58\u6570\u636e\u90fd\u5b58\u50a8\u5728\u53d8\u91cf<span class=\"code-snippet__meta-string\">\"rt\"<\/span>\u4e2d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\"># \u9996\u5148\uff0c\u68c0\u67e5\u5f53\u524dmiRNA\u8868\u8fbe\u6570\u636e\u7684\u6807\u51c6\u5dee\u662f\u5426\u5c0f\u4e8e0.1\uff0c\u5982\u679c\u662f\uff0c\u5219\u8df3\u8fc7\u8be5miRNA\u7684#\u5206\u6790\uff0c\u7ee7\u7eed\u4e0b\u4e00\u4e2amiRNA\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u63d0\u53d6\u5f53\u524dmiRNA\u7684\u76f8\u5173\u6570\u636e\uff08<span class=\"code-snippet__meta-string\">\"futime\"<\/span>\u4e3a\u751f\u5b58\u65f6\u95f4\uff0c<span class=\"code-snippet__meta-string\">\"fustat\"<\/span>\u4e3a\u751f\u5b58\u72b6\u6001\uff0c<span class=\"code-snippet__meta-string\">\"gene\"<\/span>\u4e3amiRNA<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u7684\u8868\u8fbe\u6570\u636e\uff09\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\"># \u4f7f\u7528<span class=\"code-snippet__meta-string\">\"surv_cutpoint\"<\/span>\u51fd\u6570\u8ba1\u7b97\u6700\u4f18\u622a\u65ad\u70b9\uff0c\u5c06\u60a3\u8005\u6839\u636emiRNA\u7684\u8868\u8fbe\u503c\u5206\u4e3a\u9ad8\u548c\u4f4e\u8868\u8fbe\u7ec4\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u4f7f\u7528<span class=\"code-snippet__meta-string\">\"surv_categorize\"<\/span>\u51fd\u6570\u5c06\u6570\u636e\u8fdb\u884c\u5206\u7ec4\u5e76\u8f6c\u6362\u4e3a\u9002\u5408\u751f\u5b58\u5206\u6790\u7684\u683c\u5f0f\u3002<span class=\"code-snippet__meta-string\">\"survfit\"<\/span>\u51fd\u6570\u62df#\u5408\u751f\u5b58\u66f2\u7ebf\u3002<span class=\"code-snippet__meta-string\">\"survdiff\"<\/span>\u51fd\u6570\u6bd4\u8f83\u9ad8\u4f4e\u8868\u8fbe\u7ec4\u4e4b\u95f4\u7684\u751f\u5b58\u5dee\u5f02\uff0c\u5f97\u5230p\u503c\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\"># \u6839\u636ep\u503c\u7684\u5927\u5c0f\uff0c\u5c06p\u503c\u7684\u663e\u793a\u683c\u5f0f\u6539\u4e3a<span class=\"code-snippet__meta-string\">\"p<0.001\"<\/span>\u6216<span class=\"code-snippet__meta-string\">\"p=xxx\"<\/span>\uff08\u5c0f\u6570\u70b9\u540e\u4e09\u4f4d\uff09\u3002\u4f7f\u7528#<span class=\"code-snippet__meta-string\">\"ggsurvplot\"<\/span>\u51fd\u6570\u7ed8\u5236\u751f\u5b58\u66f2\u7ebf\uff0c\u5176\u4e2d\u5305\u62ecp\u503c\u7684\u663e\u793a\u3001\u56fe\u4f8b\u7b49\u3002\u5c06\u751f\u5b58\u66f2\u7ebf\u4fdd\u5b58\u4e3aPDF\u6587#\u4ef6\uff0c\u6587\u4ef6\u540d\u7c7b\u4f3c<span class=\"code-snippet__meta-string\">\"sur.<miRNA>.pdf\"<\/span>\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\"># \u5faa\u73af\u7ee7\u7eed\u5904\u7406\u4e0b\u4e00\u4e2amiRNA\u3002\u5faa\u73af\u7ed3\u675f\u540e\uff0c\u5c06<span class=\"code-snippet__meta-string\">\"outTab\"<\/span>\u6570\u636e\u6846\u6309\u7167p\u503c\u7684\u5927\u5c0f\u8fdb\u884c\u6392<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5e8f\uff0c\u5e76\u5c06\u7ed3\u679c\u4fdd\u5b58\u4e3a\u540d\u4e3a<span class=\"code-snippet__meta-string\">\"survival.txt\"<\/span>\u7684\u5236\u8868\u7b26\u5206\u9694\u6587\u672c\u6587\u4ef6\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">outTab=outTab[order(as.numeric(as.vector(outTab[,<span class=\"code-snippet__string\">\"pValue\"<\/span>]))),]<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(outTab, file=<span class=\"code-snippet__string\">\"survival.txt\"<\/span>, sep=<span class=\"code-snippet__string\">\"t\"<\/span>, row.names=F, quote=F)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><strong style=\"font-size: 16px;letter-spacing: 0.578px;text-align: center;text-wrap: wrap;\"><\/strong><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.2337164750957854\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_dH6nZUKOmkjNQ14WicFzgKT7pfYh9w.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiaLtL4YUJdmeMiaDeibZfGoNd5gwNdH6nZUKOmkjNQ14WicFzgKT7pfYh9w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"261\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3515625\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_hmn4qcUiaRMXH0cR90zVOdd6rZrxqQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiaWedOtzwhjxwALkclyrKDRd4xShmn4qcUiaRMXH0cR90zVOdd6rZrxqQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"640\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7993827160493827\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_voFLmcr5m6BL9uZBoGc8MVH6z6TBtQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiac5NOXFibfUQ2aSJD1dUkbq9GPvoFLmcr5m6BL9uZBoGc8MVH6z6TBtQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"648\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8370607028753994\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_AkjTMvrQfmT0cZpCKedwyClkLOG8dA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiaiaK54oHCcNNEYfkZrLrRyuB7XAkjTMvrQfmT0cZpCKedwyClkLOG8dA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"626\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8762057877813505\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_CSV0vV9NkRnuich9iaUpZ1C6VS9OHg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiarFicocFp2X9pCoz1CHHNKmeUbOWCSV0vV9NkRnuich9iaUpZ1C6VS9OHg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"622\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0b\u671f\u5c06\u4e3a\u4f60\u5e26\u6765\u66f4\u591aR\u8bed\u8a00\u7684\u9a9a\u64cd\u4f5c\u6280\u5de7\uff0c\u4ee5\u4e0b\u63a8\u8350\u7684\u662f\u4e00\u4e2a\u591a\u529f\u80fd\u7684\u751f\u4fe1\u5e73\u53f0\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"color: rgb(255, 0, 0);background: rgb(255, 255, 255);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e91\u751f\u4fe1\u5e73\u53f0\u94fe\u63a5\uff1a<\/span><span style=\"color: rgb(255, 0, 0);background: rgb(255, 255, 255);text-decoration: underline;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">http:\/\/www.biocloudservice.com\/home.html\u3002<\/span><span style=\"color: rgb(255, 0, 0);background: rgb(255, 255, 255);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"color: rgb(255, 0, 0);background: rgb(255, 255, 255);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e91\u751f\u4fe1\u5e73\u53f0\u94fe\u63a5\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002<\/span><span style=\"\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><span style=\"color: rgb(217, 33, 66);\"><span style=\"font-size: 10.5pt;font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u5176\u4ed6<\/span><span style=\"font-size: 10.5pt;font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u76f8\u5173\u5206\u6790\u5185\u5bb9\uff0c\u4f8b\u5982\u9884\u6d4b\u80bf\u7624\u6837\u672c\u836f\u7269\u654f\u611f\u6027\u5206\u6790(http:\/\/www.biocloudservice.com\/712\/712.php),\u9884\u6d4b\u67d0\u6837\u672c\u4e9a\u578b\u5bf9\u514d\u75ab\u6cbb\u7597\u7684\u53cd\u5e94(http:\/\/www.biocloudservice.com\/292\/292.php)\uff0c\u5355\u6837\u672c\u5bcc\u96c6\u7b97\u6cd5\u5206\u6790\u514d\u75ab\u6d78\u6da6\u4e30\u5ea6(http:\/\/www.biocloudservice.com\/106\/106.php)\uff0c\u8ba1\u7b9764\u79cd\u514d\u75ab\u7ec6\u80de\u76f8\u5bf9\u542b\u91cf(http:\/\/www.biocloudservice.com\/107\/107.php)\u7b49\u90fd\u53ef\u4ee5\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u4e91\u5e73\u53f0\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\uff0c\u4e00\u952e\u5b8c\u6210\u8be5\u5206\u6790\u5965\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u5c1d\u8bd5\u5965\uff0c\u7f51\u5740\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002<\/span><\/span><span style=\"color: rgb(217, 33, 66);font-size: 10.5pt;font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><\/span><span style=\"mso-spacerun:"yes";font-family:\u5fae\u8f6f\u96c5\u9ed1;font-size:10.5000pt;mso-font-kerning:1.0000pt;\">\u4eca\u5929\u5c0f\u679c\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\uff0c\u4e0b\u671f\u5728\u89c1\u5965\u3002<\/span><span style=\"text-align: center;letter-spacing: 0.034em;\"><\/span><\/p>\n<section data-mpa-powered-by=\"yiban.io\" style=\"margin-bottom: 0px;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;text-align: left;white-space: normal;background-color: rgb(255, 255, 255);letter-spacing: 0.578px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 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powered-by=\"xiumi.us\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 29.9652%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 29.9652% !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;line-height: 0;\">\n<section>\n<section style=\"display: inline-block;vertical-align: middle;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 0;\">\n<section style=\"width: 111px;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 1.75em;text-indent: 0em;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;height: auto;width: 111px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_GBhsibRAgNiaQsH4ichdP9ibEhHMFA-83.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYngnlT6jiavu08Bvp2iblzGf3IkoBD9NGBhsibRAgNiaQsH4ichdP9ibEhHMFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"400\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section style=\"display: contents;\" powered-by=\"xiumi.us\">\n<section style=\"display: contents;\">\n<section style=\"display: contents;\">\n<section style=\"height: 0px;overflow: hidden;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;caret-color: red;\"> <\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5fae\u5c0fRNA\u7684\u8868\u8fbe\u6570\u636e\u4e0e\u60a3\u8005\u7684\u751f\u5b58\u6570\u636e\u8fdb\u884c\u5206\u6790\uff0c\u4ee5\u5bfb\u627e\u4e0e\u60a3\u8005\u751f\u5b58\u76f8\u5173\u7684miRNA\u3002\u751f\u5b58\u5206\u6790\u548c\u7ed8\u5236\u751f\u5b58\u66f2\u7ebf\uff0c\u627e\u51fa\u4e0e\u60a3\u8005\u751f\u5b58\u76f8\u5173\u7684miRNA\u3002\u8fd9\u5bf9\u4e8e\u4e86\u89e3miRNA\u5728\u764c\u75c7\u7b49\u75be\u75c5\u9884\u540e\u4e2d\u7684\u4f5c\u7528\u4ee5\u53ca\u5bfb\u627e\u6f5c\u5728\u7684\u751f\u7269\u6807\u5fd7\u7269\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002\u751f\u5b58\u5206\u6790\u662f\u4e00\u79cd\u5e38\u7528\u7684\u7edf\u8ba1\u5206\u6790\u65b9\u6cd5\uff0c\u53ef\u7528\u4e8e\u8bc4\u4f30\u4e0d\u540c\u57fa\u56e0\u6216\u751f\u7269\u5206\u5b50\u5728\u60a3\u8005\u9884\u540e\u4e2d\u7684\u6f5c\u5728\u5f71\u54cd\u3002\u901a\u8fc7\u8be5\u811a\u672c\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u5feb\u901f\u800c\u5168\u9762\u5730\u5bf9\u5927\u89c4\u6a21miRNA\u8868\u8fbe\u6570\u636e\u8fdb\u884c\u751f\u5b58\u76f8\u5173\u5206\u6790\uff0c\u4ece\u800c\u8f85\u52a9\u764c\u75c7\u7b49\u75be\u75c5\u7684\u7814\u7a76\u548c\u8bca\u65ad\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong><span style=\"font-size: 12pt;\">\u4ee3\u7801\u4e2d\u5bf9<\/span><\/strong><strong><span style=\"font-size: 12pt;\">\u9009\u5b9a\u7684\u76f8\u5173<\/span><\/strong><strong><span style=\"font-size: 12pt;\">miRNA\u7684\u8868\u8fbe\u91cf\u8fdb\u884c<\/span><\/strong><strong><span style=\"font-size: 12pt;\">\u751f\u5b58\u5206\u6790<\/span><\/strong><strong><span style=\"font-size: 12pt;\">\u3002<\/span><\/strong><strong><span style=\"font-size: 12pt;\">\u4e0b\u9762\u662f\u8981\u7814\u7a76\u7684\u591a\u4e2amirna\u7684\u6837\u672c\u8868\u8fbe\u91cf\uff0c\u4ee5\u53ca\u4e0b\u8f7d\u7684STAD\u764c\u75c7\u7684\u751f\u5b58\u65f6\u95f4\u548c\u72b6\u6001\u3002<\/span><\/strong><\/span><strong><span style=\"font-size: 12pt;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><\/span><\/strong><\/p>\n<p><strong><span style=\"font-size: 12pt;font-family: \u5b8b\u4f53;\"><br \/><\/span><\/strong><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2745664739884393\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_MDic1Xkt7E6HGtHpovic26CviciacA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiafuKNiajYIicLGASWOa3Yu6WxLsnwlWMDic1Xkt7E6HGtHpovic26CviciacA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"692\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9875259875259875\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_QNwwIymk7FtWRhQqc05gtibf59ogcw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiaKYYVfuKnNgQFG5oT2x9Y0mRfbQNwwIymk7FtWRhQqc05gtibf59ogcw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"481\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: center;white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span><span class=\"code-snippet__keyword\">if<\/span> (!requireNamespace(<span class=\"code-snippet__string\">\"BiocManager\"<\/span>, quietly = TRUE))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span> install.packages(<span class=\"code-snippet__string\">\"BiocManager\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span>BiocManager::install(<span class=\"code-snippet__string\">\"limma\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u68c0\u67e5\u662f\u5426\u5df2\u5b89\u88c5”BiocManager”\u5305\uff0c\u5982\u679c\u6ca1\u6709\u5b89\u88c5\uff0c\u5219\u901a\u8fc7install.packages\u51fd\u6570\u5b89\u88c5\u5b83\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">“BiocManager”\u5305\u7528\u4e8e\u7ba1\u7406\u548c\u5b89\u88c5\u751f\u7269\u4fe1\u606f\u5b66\u76f8\u5173\u7684\u5176\u4ed6\u5305\u3002<\/span><\/p>\n<p style=\"text-align: center;white-space: normal;font-size: 16px;\"><o:p><\/o:p><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span>install.packages(<span class=\"code-snippet__string\">'survival'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#<\/span>install.packages(<span class=\"code-snippet__string\">\"survminer\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5b89\u88c5”survminer”\u5305\uff0c\u5b83\u662f\u4e00\u4e2a\u7528\u4e8e\u751f\u5b58\u6570\u636e\u53ef\u89c6\u5316\u548c\u7edf\u8ba1\u5206\u6790\u7684\u5305\u3002<span style=\"font-size: 14px;font-family: \u5b8b\u4f53;\"><\/span><\/span><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code><span class=\"code-snippet_outer\">library(limma)<\/span><\/code><code><span class=\"code-snippet_outer\">library(survival)<\/span><\/code><code><span class=\"code-snippet_outer\">library(survminer)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">expFile=<span class=\"code-snippet__string\">\"cor.MirnaExp.txt\"<\/span> <span class=\"code-snippet__meta\">#\u5171\u8868\u8fbemiRNA\u7684\u8868\u8fbe\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cliFile=<span class=\"code-snippet__string\">\"TCGA-STAD.survival.tsv\"<\/span> <span class=\"code-snippet__meta\">#\u751f\u5b58\u6570\u636e\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u6307\u5b9a\u4e86miRNA\u8868\u8fbe\u6570\u636e\u6587\u4ef6\u548c\u751f\u5b58\u6570\u636e\u6587\u4ef6\u5e76\u5c06\u5b83\u4eec\u5206\u522b\u8d4b\u503c\u7ed9\u53d8\u91cf<span class=\"code-snippet__meta-string\">\"expFile\"<\/span>\u548c<span class=\"code-snippet__meta-string\">\"cliFile\"<\/span><\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8bfb\u53d6\u8868\u8fbe\u6587\u4ef6\uff0c\u5e76\u5bf9\u8f93\u5165\u6587\u4ef6\u6574\u7406<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=read.table(expFile, header=T, sep=<span class=\"code-snippet__string\">\"t\"<\/span>, check.names=F, row.names=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u4f7f\u7528read.table\u51fd\u6570\u8bfb\u53d6\u540d\u4e3a<span class=\"code-snippet__meta-string\">\"cor.MirnaExp.txt\"<\/span>\u7684miRNA\u8868\u8fbe\u6587\u4ef6\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5220\u6389\u6b63\u5e38\u6837\u54c1<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(colnames(data),<span class=\"code-snippet__string\">\"\\-\"<\/span>), <span class=\"code-snippet__string\">\"[\"<\/span>, <span class=\"code-snippet__number\">4<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(group,<span class=\"code-snippet__string\">\"\"<\/span>), <span class=\"code-snippet__string\">\"[\"<\/span>, <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">group=gsub(<span class=\"code-snippet__string\">\"2\"<\/span>, <span class=\"code-snippet__string\">\"1\"<\/span>, group)<\/span><\/code><code><span class=\"code-snippet_outer\">data=t(data[,group==<span class=\"code-snippet__number\">0<\/span>,drop=F])<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(data)=gsub(<span class=\"code-snippet__string\">\"(.*?)\\-(.*?)\\-(.*?)\\-(.*?)\\-.*\"<\/span>, <span class=\"code-snippet__string\">\"\\1\\-\\2\\-\\3-\\4\"<\/span>, rownames(data))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5bf9\u8bfb\u53d6\u7684miRNA\u8868\u8fbe\u6570\u636e\u8fdb\u884c\u9884\u5904\u7406\u3002\u9996\u5148\uff0c\u4ece\u6837\u672c\u540d\u4e2d\u63d0\u53d6\u6570\u5b57\u90e8\u5206\u4ee5\u4fbf\u533a\u5206\u6b63\u5e38\u6837\u672c#\u548c\u80bf\u7624\u6837\u672c\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u7136\u540e\uff0c\u6839\u636e<span class=\"code-snippet__meta-string\">\"group\"<\/span>\u53d8\u91cf\u4e2d\u7684\u503c\uff0c\u5c06\u6b63\u5e38\u6837\u672c\u5bf9\u5e94\u7684\u5217\u4ece\u6570\u636e\u4e2d\u5220\u9664\u3002\u6700\u540e\uff0c\u6839\u636e\u6b63\u5219\u8868\u8fbe#\u5f0f\uff0c\u5c06\u884c\u540d\u7684\u6837\u672c\u7f16\u53f7\u8fdb\u884c\u8c03\u6574\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8bfb\u53d6\u751f\u5b58\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cli=read.table(cliFile, header=T, sep=<span class=\"code-snippet__string\">\"t\"<\/span>, check.names=F, row.names=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">cli=cli[,c(<span class=\"code-snippet__number\">3<\/span>,<span class=\"code-snippet__number\">1<\/span>)]<\/span><\/code><code><span class=\"code-snippet_outer\">cli=na.omit(cli)<\/span><\/code><code><span class=\"code-snippet_outer\">colnames(cli)=c(<span class=\"code-snippet__string\">\"futime\"<\/span>,<span class=\"code-snippet__string\">\"fustat\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">cli$futime=cli$futime\/<span class=\"code-snippet__number\">365<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8bfb\u53d6\u540d\u4e3a<span class=\"code-snippet__meta-string\">\"TCGA-STAD.survival.tsv\"<\/span>\u7684\u751f\u5b58\u6570\u636e\u6587\u4ef6\u3002\u6587\u4ef6\u4ee5\u5236\u8868\u7b26\u5206\u9694\uff0c\u7b2c\u4e00\u884c\u662f\u5217\u540d\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u6240\u4ee5header=T\u8868\u793a\u7b2c\u4e00\u884c\u662f\u5217\u540d\u3002sep=<span class=\"code-snippet__meta-string\">\"t\"<\/span>\u8868\u793a\u6587\u4ef6\u4e2d\u7684\u5b57\u6bb5\u662f\u7528\u5236\u8868\u7b26\u5206\u9694\u7684\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">check.names=F\u8868\u793a\u4e0d\u68c0\u67e5\u5217\u540d\u7684\u5408\u6cd5\u6027\u3002row.names=<span class=\"code-snippet__number\">1<\/span>\u8868\u793a\u4f7f\u7528\u7b2c\u4e00\u5217\u4f5c\u4e3a\u884c\u540d\u3002<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u751f\u5b58\u6570\u636e\u6587\u4ef6\u4e2d\u7684\u5217\u610f\u4e49\u5206\u522b\u662f\uff1a\u7b2c1\u5217\u662f\u6837\u672cID\uff0c\u7b2c3\u5217\u662f\u751f\u5b58\u65f6\u95f4\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u7b2c2\u5217\u662f\u751f\u5b58\u72b6\u6001\uff080\u8868\u793a\u751f\u5b58\uff0c1\u8868\u793a\u6b7b\u4ea1\uff09\u3002\u63a5\u4e0b\u6765\u7684\u4ee3\u7801\u5c06\u8bfb\u53d6\u7684\u751f\u5b58\u6570\u636e\u8fdb\u884c\u5904\u7406\uff1a<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u63d0\u53d6\u7b2c1\u5217\u548c\u7b2c3\u5217\uff08\u751f\u5b58\u65f6\u95f4\u548c\u751f\u5b58\u72b6\u6001\uff09\uff0c\u5220\u9664\u53ef\u80fd\u5305\u542b\u7f3a\u5931\u503c\u7684\u884c\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5e76\u5c06\u5217\u540d\u6539\u4e3a<span class=\"code-snippet__meta-string\">\"futime\"<\/span>\u548c<span class=\"code-snippet__meta-string\">\"fustat\"<\/span>\u3002\u6700\u540e\uff0c\u5c06\u751f\u5b58\u65f6\u95f4\u8f6c\u6362\u4e3a\u5e74\u4e3a\u5355\u4f4d\uff08\u9664\u4ee5365\uff09\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u6570\u636e\u5408\u5e76<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sameSample=intersect(row.names(data), row.names(cli))<\/span><\/code><code><span class=\"code-snippet_outer\">data=data[sameSample,,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">cli=cli[sameSample,,drop=F]<\/span><\/code><code><span class=\"code-snippet_outer\">rt=cbind(cli, data)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u8bfb\u53d6\u540d\u4e3a<span class=\"code-snippet__meta-string\">\"TCGA-STAD.survival.tsv\"<\/span>\u7684\u751f\u5b58\u6570\u636e\u6587\u4ef6\u3002\u6587\u4ef6\u4ee5\u5236\u8868\u7b26\u5206\u9694\uff0c\u7b2c\u4e00\u884c\u662f\u5217\u540d\uff0c\u6240#\u4ee5header=T\u8868\u793a\u7b2c\u4e00\u884c\u662f\u5217\u540d\u3002sep=<span class=\"code-snippet__meta-string\">\"t\"<\/span>\u8868\u793a\u6587\u4ef6\u4e2d\u7684\u5b57\u6bb5\u662f\u7528\u5236\u8868\u7b26\u5206\u9694\u7684\u3002check.names=F\u8868\u793a\u4e0d\u68c0\u67e5\u5217\u540d\u7684\u5408\u6cd5\u6027\u3002row.names=1\u8868\u793a\u4f7f\u7528\u7b2c\u4e00\u5217\u4f5c\u4e3a\u884c\u540d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u751f\u5b58\u6570\u636e\u6587\u4ef6\u4e2d\u7684\u5217\u610f\u4e49\u5206\u522b\u662f\uff1a\u7b2c1\u5217\u662f\u6837\u672cID\uff0c\u7b2c3\u5217\u662f\u751f\u5b58\u65f6\u95f4\uff0c\u7b2c2\u5217\u662f\u751f\u5b58\u72b6#\u6001\uff080\u8868\u793a\u751f\u5b58\uff0c1\u8868\u793a\u6b7b\u4ea1\uff09\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u63a5\u4e0b\u6765\u7684\u4ee3\u7801\u5c06\u8bfb\u53d6\u7684\u751f\u5b58\u6570\u636e\u8fdb\u884c\u5904\u7406\uff1a\u63d0\u53d6\u7b2c1\u5217\u548c\u7b2c3\u5217\uff08\u751f\u5b58\u65f6\u95f4\u548c\u751f\u5b58\u72b6\u6001\uff09\uff0c#\u5220\u9664\u53ef\u80fd\u5305\u542b\u7f3a\u5931\u503c\u7684\u884c\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5e76\u5c06\u5217\u540d\u6539\u4e3a<span class=\"code-snippet__meta-string\">\"futime\"<\/span>\u548c<span class=\"code-snippet__meta-string\">\"fustat\"<\/span>\u3002\u6700\u540e\uff0c\u5c06\u751f\u5b58\u65f6\u95f4\u8f6c\u6362\u4e3a\u5e74\u4e3a\u5355\u4f4d\uff08\u9664\u4ee5365\uff09\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5bf9miRNA\u8fdb\u884c\u5faa\u73af\uff0c\u627e\u51fa\u9884\u540e\u76f8\u5173\u7684miRNA<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">outTab=data.frame()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u521b\u5efa\u4e00\u4e2a\u7a7a\u7684\u6570\u636e\u6846<span class=\"code-snippet__meta-string\">\"outTab\"<\/span>\u6765\u4fdd\u5b58\u6bcf\u4e2amiRNA\u7684\u5206\u6790\u7ed3\u679c\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(gene <span class=\"code-snippet__keyword\">in<\/span> colnames(rt)[<span class=\"code-snippet__number\">3<\/span>:ncol(rt)]){<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\">#\u63d0\u53d6miRNA\u4fe1\u606f<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">if<\/span>(sd(rt[,gene])<<span class=\"code-snippet__number\">0.1<\/span>){next}<\/span><\/code><code><span class=\"code-snippet_outer\"> data=rt[,c(<span class=\"code-snippet__string\">\"futime\"<\/span>, <span class=\"code-snippet__string\">\"fustat\"<\/span>, gene)]<\/span><\/code><code><span class=\"code-snippet_outer\"> colnames(data)=c(<span class=\"code-snippet__string\">\"futime\"<\/span>, <span class=\"code-snippet__string\">\"fustat\"<\/span>, <span class=\"code-snippet__string\">\"gene\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\">#\u83b7\u53d6\u6700\u4f18cutoff,\u5e76\u5bf9\u6837\u54c1\u8fdb\u884c\u5206\u7ec4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> res.cut=surv_cutpoint(data, time = <span class=\"code-snippet__string\">\"futime\"<\/span>, event = <span class=\"code-snippet__string\">\"fustat\"<\/span>, variables =c(<span class=\"code-snippet__string\">\"gene\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"> res.cat=surv_categorize(res.cut)<\/span><\/code><code><span class=\"code-snippet_outer\"> fit=survfit(Surv(futime, fustat) ~gene, data = res.cat)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\">#\u6bd4\u8f83\u9ad8\u4f4e\u8868\u8fbe\u7ec4\u751f\u5b58\u5dee\u5f02<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> diff=survdiff(Surv(futime, fustat) ~gene, data =res.cat)<\/span><\/code><code><span class=\"code-snippet_outer\"> pValue=<span class=\"code-snippet__number\">1<\/span>-pchisq(diff$chisq, df=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> outVector=cbind(gene, res.cut$cutpoint[<span class=\"code-snippet__number\">1<\/span>], pValue)<\/span><\/code><code><span class=\"code-snippet_outer\"> outTab=rbind(outTab,outVector)<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">if<\/span>(pValue<<span class=\"code-snippet__number\">0.001<\/span>){<\/span><\/code><code><span class=\"code-snippet_outer\"> pValue=<span class=\"code-snippet__string\">\"p<0.001\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> }<span class=\"code-snippet__keyword\">else<\/span>{<\/span><\/code><code><span class=\"code-snippet_outer\"> pValue=paste0(<span class=\"code-snippet__string\">\"p=\"<\/span>,sprintf(<span class=\"code-snippet__string\">\"%.03f\"<\/span>,pValue))<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\">#\u7ed8\u5236\u751f\u5b58\u66f2\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> surPlot=ggsurvplot(fit,<\/span><\/code><code><span class=\"code-snippet_outer\"> data=res.cat,<\/span><\/code><code><span class=\"code-snippet_outer\"> pval=pValue,<\/span><\/code><code><span class=\"code-snippet_outer\"> pval.size=<span class=\"code-snippet__number\">6<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.title=gene,<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.labs=c(<span class=\"code-snippet__string\">\"high\"<\/span>,<span class=\"code-snippet__string\">\"low\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab=<span class=\"code-snippet__string\">\"Time(years)\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> ylab=<span class=\"code-snippet__string\">\"Overall survival\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> palette=c(<span class=\"code-snippet__string\">\"red\"<\/span>, <span class=\"code-snippet__string\">\"blue\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__keyword\">break<\/span>.time.by=<span class=\"code-snippet__number\">1<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> conf.int=T,<\/span><\/code><code><span class=\"code-snippet_outer\"> risk.table=F,<\/span><\/code><code><span class=\"code-snippet_outer\"> risk.table.title=<span class=\"code-snippet__string\">\"\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> risk.table.height=<span class=\"code-snippet__number\">.25<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\">#\u8f93\u51fa\u56fe\u5f62<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> pdf(file=paste0(<span class=\"code-snippet__string\">\"sur.\"<\/span>,gene,<span class=\"code-snippet__string\">\".pdf\"<\/span>),onefile = <span class=\"code-snippet__literal\">FALSE<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> width = <span class=\"code-snippet__number\">5.5<\/span>, <span class=\"code-snippet__meta\">#\u56fe\u7247\u7684\u5bbd\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> height =<span class=\"code-snippet__number\">5<\/span>) <span class=\"code-snippet__meta\">#\u56fe\u7247\u7684\u9ad8\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> print(surPlot)<\/span><\/code><code><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5faa\u73af\u5bf9\u6bcf\u4e2amiRNA\u8fdb\u884c\u751f\u5b58\u5206\u6790\uff0c\u5e76\u7ed8\u5236\u751f\u5b58\u66f2\u7ebf\u3002\u4e3b\u8981\u6b65\u9aa4\u5982\u4e0b\uff1a<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\"># \u4f7f\u7528<span class=\"code-snippet__meta-string\">\"for\"<\/span>\u5faa\u73af\u904d\u5386\u4ece\u7b2c3\u5217\u5f00\u59cb\u5230\u6700\u540e\u4e00\u5217\u7684\u6240\u6709miRNA\u7684\u5217\u540d\u3002\u5728\u8fd9\u91cc\uff0c\u6bcf\u4e2amiRNA<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u7684\u8868\u8fbe\u6570\u636e\u548c\u751f\u5b58\u6570\u636e\u90fd\u5b58\u50a8\u5728\u53d8\u91cf<span class=\"code-snippet__meta-string\">\"rt\"<\/span>\u4e2d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\"># \u9996\u5148\uff0c\u68c0\u67e5\u5f53\u524dmiRNA\u8868\u8fbe\u6570\u636e\u7684\u6807\u51c6\u5dee\u662f\u5426\u5c0f\u4e8e0.1\uff0c\u5982\u679c\u662f\uff0c\u5219\u8df3\u8fc7\u8be5miRNA\u7684#\u5206\u6790\uff0c\u7ee7\u7eed\u4e0b\u4e00\u4e2amiRNA\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u63d0\u53d6\u5f53\u524dmiRNA\u7684\u76f8\u5173\u6570\u636e\uff08<span class=\"code-snippet__meta-string\">\"futime\"<\/span>\u4e3a\u751f\u5b58\u65f6\u95f4\uff0c<span class=\"code-snippet__meta-string\">\"fustat\"<\/span>\u4e3a\u751f\u5b58\u72b6\u6001\uff0c<span class=\"code-snippet__meta-string\">\"gene\"<\/span>\u4e3amiRNA<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u7684\u8868\u8fbe\u6570\u636e\uff09\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\"># \u4f7f\u7528<span class=\"code-snippet__meta-string\">\"surv_cutpoint\"<\/span>\u51fd\u6570\u8ba1\u7b97\u6700\u4f18\u622a\u65ad\u70b9\uff0c\u5c06\u60a3\u8005\u6839\u636emiRNA\u7684\u8868\u8fbe\u503c\u5206\u4e3a\u9ad8\u548c\u4f4e\u8868\u8fbe\u7ec4\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u4f7f\u7528<span class=\"code-snippet__meta-string\">\"surv_categorize\"<\/span>\u51fd\u6570\u5c06\u6570\u636e\u8fdb\u884c\u5206\u7ec4\u5e76\u8f6c\u6362\u4e3a\u9002\u5408\u751f\u5b58\u5206\u6790\u7684\u683c\u5f0f\u3002<span class=\"code-snippet__meta-string\">\"survfit\"<\/span>\u51fd\u6570\u62df#\u5408\u751f\u5b58\u66f2\u7ebf\u3002<span class=\"code-snippet__meta-string\">\"survdiff\"<\/span>\u51fd\u6570\u6bd4\u8f83\u9ad8\u4f4e\u8868\u8fbe\u7ec4\u4e4b\u95f4\u7684\u751f\u5b58\u5dee\u5f02\uff0c\u5f97\u5230p\u503c\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\"># \u6839\u636ep\u503c\u7684\u5927\u5c0f\uff0c\u5c06p\u503c\u7684\u663e\u793a\u683c\u5f0f\u6539\u4e3a<span class=\"code-snippet__meta-string\">\"p<0.001\"<\/span>\u6216<span class=\"code-snippet__meta-string\">\"p=xxx\"<\/span>\uff08\u5c0f\u6570\u70b9\u540e\u4e09\u4f4d\uff09\u3002\u4f7f\u7528#<span class=\"code-snippet__meta-string\">\"ggsurvplot\"<\/span>\u51fd\u6570\u7ed8\u5236\u751f\u5b58\u66f2\u7ebf\uff0c\u5176\u4e2d\u5305\u62ecp\u503c\u7684\u663e\u793a\u3001\u56fe\u4f8b\u7b49\u3002\u5c06\u751f\u5b58\u66f2\u7ebf\u4fdd\u5b58\u4e3aPDF\u6587#\u4ef6\uff0c\u6587\u4ef6\u540d\u7c7b\u4f3c<span class=\"code-snippet__meta-string\">\"sur.<miRNA>.pdf\"<\/span>\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__meta\"># \u5faa\u73af\u7ee7\u7eed\u5904\u7406\u4e0b\u4e00\u4e2amiRNA\u3002\u5faa\u73af\u7ed3\u675f\u540e\uff0c\u5c06<span class=\"code-snippet__meta-string\">\"outTab\"<\/span>\u6570\u636e\u6846\u6309\u7167p\u503c\u7684\u5927\u5c0f\u8fdb\u884c\u6392<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u5e8f\uff0c\u5e76\u5c06\u7ed3\u679c\u4fdd\u5b58\u4e3a\u540d\u4e3a<span class=\"code-snippet__meta-string\">\"survival.txt\"<\/span>\u7684\u5236\u8868\u7b26\u5206\u9694\u6587\u672c\u6587\u4ef6\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">outTab=outTab[order(as.numeric(as.vector(outTab[,<span class=\"code-snippet__string\">\"pValue\"<\/span>]))),]<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(outTab, file=<span class=\"code-snippet__string\">\"survival.txt\"<\/span>, sep=<span class=\"code-snippet__string\">\"t\"<\/span>, row.names=F, quote=F)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><strong style=\"font-size: 16px;letter-spacing: 0.578px;text-align: center;text-wrap: wrap;\"><\/strong><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.2337164750957854\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_dH6nZUKOmkjNQ14WicFzgKT7pfYh9w.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiaLtL4YUJdmeMiaDeibZfGoNd5gwNdH6nZUKOmkjNQ14WicFzgKT7pfYh9w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"261\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3515625\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_hmn4qcUiaRMXH0cR90zVOdd6rZrxqQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiaWedOtzwhjxwALkclyrKDRd4xShmn4qcUiaRMXH0cR90zVOdd6rZrxqQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"640\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7993827160493827\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_voFLmcr5m6BL9uZBoGc8MVH6z6TBtQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiac5NOXFibfUQ2aSJD1dUkbq9GPvoFLmcr5m6BL9uZBoGc8MVH6z6TBtQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"648\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8370607028753994\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_AkjTMvrQfmT0cZpCKedwyClkLOG8dA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiaiaK54oHCcNNEYfkZrLrRyuB7XAkjTMvrQfmT0cZpCKedwyClkLOG8dA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"626\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8762057877813505\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_CSV0vV9NkRnuich9iaUpZ1C6VS9OHg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxG8CiahcYUTiaHhRf8ibykdiaiarFicocFp2X9pCoz1CHHNKmeUbOWCSV0vV9NkRnuich9iaUpZ1C6VS9OHg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"622\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0b\u671f\u5c06\u4e3a\u4f60\u5e26\u6765\u66f4\u591aR\u8bed\u8a00\u7684\u9a9a\u64cd\u4f5c\u6280\u5de7\uff0c\u4ee5\u4e0b\u63a8\u8350\u7684\u662f\u4e00\u4e2a\u591a\u529f\u80fd\u7684\u751f\u4fe1\u5e73\u53f0\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"color: rgb(255, 0, 0);background: rgb(255, 255, 255);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e91\u751f\u4fe1\u5e73\u53f0\u94fe\u63a5\uff1a<\/span><span style=\"color: rgb(255, 0, 0);background: rgb(255, 255, 255);text-decoration: underline;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">http:\/\/www.biocloudservice.com\/home.html\u3002<\/span><span style=\"color: rgb(255, 0, 0);background: rgb(255, 255, 255);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"color: rgb(255, 0, 0);background: rgb(255, 255, 255);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e91\u751f\u4fe1\u5e73\u53f0\u94fe\u63a5\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002<\/span><span style=\"\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><span style=\"color: rgb(217, 33, 66);\"><span style=\"font-size: 10.5pt;font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u5176\u4ed6<\/span><span style=\"font-size: 10.5pt;font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u76f8\u5173\u5206\u6790\u5185\u5bb9\uff0c\u4f8b\u5982\u9884\u6d4b\u80bf\u7624\u6837\u672c\u836f\u7269\u654f\u611f\u6027\u5206\u6790(http:\/\/www.biocloudservice.com\/712\/712.php),\u9884\u6d4b\u67d0\u6837\u672c\u4e9a\u578b\u5bf9\u514d\u75ab\u6cbb\u7597\u7684\u53cd\u5e94(http:\/\/www.biocloudservice.com\/292\/292.php)\uff0c\u5355\u6837\u672c\u5bcc\u96c6\u7b97\u6cd5\u5206\u6790\u514d\u75ab\u6d78\u6da6\u4e30\u5ea6(http:\/\/www.biocloudservice.com\/106\/106.php)\uff0c\u8ba1\u7b9764\u79cd\u514d\u75ab\u7ec6\u80de\u76f8\u5bf9\u542b\u91cf(http:\/\/www.biocloudservice.com\/107\/107.php)\u7b49\u90fd\u53ef\u4ee5\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u4e91\u5e73\u53f0\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\uff0c\u4e00\u952e\u5b8c\u6210\u8be5\u5206\u6790\u5965\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u5c1d\u8bd5\u5965\uff0c\u7f51\u5740\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002<\/span><\/span><span style=\"color: rgb(217, 33, 66);font-size: 10.5pt;font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><\/span><span style=\"mso-spacerun:"yes";font-family:\u5fae\u8f6f\u96c5\u9ed1;font-size:10.5000pt;mso-font-kerning:1.0000pt;\">\u4eca\u5929\u5c0f\u679c\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\uff0c\u4e0b\u671f\u5728\u89c1\u5965\u3002<\/span><span style=\"text-align: center;letter-spacing: 0.034em;\"><\/span><\/p>\n<section data-mpa-powered-by=\"yiban.io\" style=\"margin-bottom: 0px;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;text-align: left;white-space: normal;background-color: rgb(255, 255, 255);letter-spacing: 0.578px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section data-role=\"list\" style=\"outline: 0px;visibility: visible;\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"outline: 0px;visibility: visible;\">\n<section style=\"padding-right: 15px;padding-left: 15px;outline: 0px;font-size: 14px;\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;height: auto;transform: perspective(0px);transform-style: flat;\">\n<section style=\"margin-top: 10px;margin-bottom: -3px;outline: 0px;justify-content: flex-start;display: flex;flex-flow: row nowrap;transform: translate3d(19px, 0px, 0px) rotateX(180deg);\">\n<section style=\"outline: 0px;display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: center;\">\n<section style=\"outline: 0px;display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 230px;background-color: rgb(217, 217, 217);\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;\">\n<p style=\"outline: 0px;\"><br style=\"outline: 0px;\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 10px;outline: 0px;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"padding: 14px 10px;outline: 0px;display: inline-block;width: 647px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;border-style: solid;border-width: 1px;border-color: rgb(217, 217, 217);\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 15px;outline: 0px;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"padding-left: 9px;outline: 0px;display: inline-block;width: 625px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 0px 12px;border-left-color: rgb(212, 32, 2);\">\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;font-size: 17px;color: rgb(62, 62, 62);\">\n<p style=\"outline: 0px;\"><strong style=\"outline: 0px;\">\u5c0f\u679c\u53cb\u60c5\u63a8\u8350<\/strong><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 3px;outline: 0px;\">\n<section style=\"outline: 0px;background-color: rgb(217, 217, 217);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;font-size: 11px;color: rgb(62, 62, 62);\">\n<p style=\"outline: 0px;\">\u597d\u7528\u53c8\u514d\u8d39\u7684\u5de5\u5177\u5b89\u5229<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section 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