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src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_noa7XnTeHKzAvzPPVsfE8hehXbojnw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5ALhzPxoTm1cec88YJ1bAoGZDnoa7XnTeHKzAvzPPVsfE8hehXbojnw\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5982\u679c\u5f15\u5165\u4e00\u4e2a\u7a7a\u683c\u6216\u7a7a\u683c\uff08gap\uff09\uff0c\u5339\u914d\u4e0a\u7684\u72b6\u51b5\u5c31\u53ef\u4ee5\u660e\u663e\u6539\u5584<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.47289156626506024\" data-s=\"300,640\" data-type=\"png\" data-w=\"332\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_DkhMhC2eibp1wjmF2gctYSic9HK1oA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q55ngfiapbaYESzujPtCNvUGZ0nxFDkhMhC2eibp1wjmF2gctYSic9HK1oA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 10.5pt;\">\u56e0\u6b64\uff0c\u5c0f\u679c\u6839\u636e\u67e5\u9605\u6587\u732e\u8ba4\u4e3a\u4ee5\u4e0b\u8bf4\u6cd5\u662f\u5e8f\u5217\u6bd4\u5bf9\u7684\u6700\u597d\u89e3\u91ca\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u6839\u636e\u7279\u5b9a\u7684\u8ba1\u5206\u89c4\u5219\uff0c\u901a\u8fc7\u4e00\u5b9a\u7684\u7b97\u6cd5\u5bf9\u4e24\u6761\u6216\u8005\u591a\u6761DNA\u6216\u8005\u86cb\u767d\u5e8f\u5217\u8fdb\u884c\u6bd4\u8f83\uff0c\u627e\u51fa\u4ed6\u4eec\u4e4b\u95f4\u7684\u6700\u4f18\u5339\u914d\u6216\u8005\u6700\u5927\u76f8\u4f3c\u5ea6\u5339\u914d\u3002\u5e8f\u5217\u5339\u914d\u7684\u610f\u4e49\u5728\u4e8e\uff0c\u901a\u8fc7\u5e8f\u5217\u6bd4\u5bf9\u53ef\u4ee5\u83b7\u5f97\u4e00\u4e2a\u5e8f\u5217\u76f8\u4f3c\u6027\u5ea6\u6bd4\u503c\uff0c\u901a\u8fc7\u8fd9\u4e2a\u503c\u53ef\u4ee5\u83b7\u5f97\u4e24\u6761\u5e8f\u5217\u76f8\u4f3c\u5ea6\u6216\u8005\u540c\u6e90\u6027\u7684\u7edf\u8ba1\u5b66\u5224\u5b9a\u7ed3\u679c\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" data-type=\"jpeg\" data-w=\"1080\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-03_\u5341\u5206\u949f\u5403\u900fblast\u6bd4\u5bf9\u5e95\u5c42\u903b\u8f91\u5e76\u5b9e\u64cd\/3_IlmBAWe69EMUPMhNEgrv6NXX85lJxg.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwyrj7kbUR3ULG9ZUbLrLkgibzL8qUpQROfe8ebc3gNSnu1dIlmBAWe69EMUPMhNEgrv6NXX85lJxg\/640?wx_fmt=jpeg'\" \/><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<h2 style=\"text-align: center;\"><em><span style=\"font-size: 20px;\"><strong><span style=\"font-family: \u9ed1\u4f53;\">\u52a8\u6001\u89c4\u5212<\/span><\/strong><\/span><\/em><strong><span style=\"mso-spacerun:'yes';font-family:\u9ed1\u4f53;mso-ascii-font-family:Arial;mso-hansi-font-family:Arial;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:16.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h2>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 10.5pt;\">\u60f3\u8981\u4e86\u89e3blastn\u6216\u8005\u5e8f\u5217\u6bd4\u5bf9\u7684\u5e95\u5c42\u903b\u8f91\uff0c\u6211\u4eec\u9700\u8981\u628a\u52a8\u6001\u89c4\u5212\u5f04\u660e\u767d\uff0c\u8bb2\u6e05\u695a\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 10.5pt;\"><br \/><\/span><\/p>\n<h3 style=\"text-align: center;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 20px;\"><em><strong>\u6253\u5206\u77e9\u9635<\/strong><\/em><\/span><strong><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:16.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h3>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u9996\u5148\uff0c\u9700\u8981\u4e86\u89e3\u4ec0\u4e48\u662f\u6253\u5206\u77e9\u9635\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5bf9\u78b1\u57fa\u5339\u914d\u60c5\u51b5\u8fdb\u884c\u4e00\u4e2a\u6807\u51c6\u5316\u7684\u77e9\u9635\uff0c\u53ef\u4ee5\u6839\u636e<o:p><\/o:p><\/span><\/p>\n<p><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">1\u3001\u66ff\u4ee3\u77e9\u9635\uff1a<\/span><\/strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">DNA\u66ff\u4ee3\u77e9\u9635\uff1a\u7528\u4e8e\u8bc4\u4f30DNA\u5e8f\u5217\u4e2d\u4e0d\u540c\u78b1\u57fa\u4e4b\u95f4\u7684\u66ff\u4ee3\u5f97\u5206\u3002\u5e38\u89c1\u7684DNA\u66ff\u4ee3\u77e9\u9635\u5305\u62ec\u7b80\u5355\u7684\u7b49\u5206\u77e9\u9635\uff08\u4f8b\u5982\uff0cA\u548cT\u4e4b\u95f4\u5f97\u5206\u4e3a1\uff0cA\u548cG\u4e4b\u95f4\u5f97\u5206\u4e3a-1\uff09\u4ee5\u53ca\u66f4\u590d\u6742\u7684\u6a21\u578b\uff0c\u5982PAM\uff08Point Accepted Mutation\uff09\u548cBLOSUM\uff08Blocks Substitution Matrix\uff09\u77e9\u9635\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u86cb\u767d\u8d28\u66ff\u4ee3\u77e9\u9635\uff1a\u7528\u4e8e\u8bc4\u4f30\u86cb\u767d\u8d28\u5e8f\u5217\u4e2d\u4e0d\u540c\u6c28\u57fa\u9178\u4e4b\u95f4\u7684\u66ff\u4ee3\u5f97\u5206\u3002\u5e38\u89c1\u7684\u86cb\u767d\u8d28\u66ff\u4ee3\u77e9\u9635\u5305\u62ecBLOSUM\u7cfb\u5217\u548cPAM\u7cfb\u5217\u77e9\u9635\u3002\u8fd9\u4e9b\u77e9\u9635\u6839\u636e\u86cb\u767d\u8d28\u5bb6\u65cf\u7684\u591a\u5e8f\u5217\u6bd4\u5bf9\u7ed3\u679c\u5efa\u7acb\uff0c\u8003\u8651\u4e86\u6c28\u57fa\u9178\u7684\u8fdb\u5316\u5173\u7cfb\u548c\u66ff\u4ee3\u9891\u7387\u3002<o:p><\/o:p><\/span><\/p>\n<p><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">2\u3001\u7f3a\u5931\u548c\u63d2\u5165\u7f5a\u5206\uff1a<\/span><\/strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">Gap\u7f5a\u5206\u77e9\u9635\uff1a\u7528\u4e8e\u60e9\u7f5a\u5e8f\u5217\u6bd4\u5bf9\u4e2d\u7684\u7f3a\u5931\uff08Gap\uff09\u548c\u63d2\u5165\u3002\u5f15\u5165Gap\u4f1a\u5bfc\u81f4\u5e8f\u5217\u4e0d\u5339\u914d\u548c\u4e0d\u5bf9\u9f50\uff0c\u4e3a\u4e86\u60e9\u7f5aGap\u7684\u5f15\u5165\uff0c\u901a\u5e38\u5728\u6253\u5206\u77e9\u9635\u4e2d\u5f15\u5165\u4e86\u5f00\u653e(Gap Opening Penalty)\u548c\u6269\u5c55(Gap Extension Penalty)\u5904\u7f5a\u5f97\u5206\u3002\u8fd9\u4e9b\u5f97\u5206\u4f1a\u968f\u7740Gap\u7684\u957f\u5ea6\u9010\u6e10\u589e\u52a0\u3002<o:p><\/o:p><\/span><\/p>\n<p><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">3\u3001\u76f4\u63a5\u5f97\u5206\u77e9\u9635\uff1a<\/span><\/strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5339\u914d-\u4e0d\u5339\u914d\u5f97\u5206\uff1a\u5728\u67d0\u4e9b\u60c5\u51b5\u4e0b\uff0c\u6253\u5206\u77e9\u9635\u4e2d\u7684\u5f97\u5206\u53ef\u4ee5\u6839\u636e\u78b1\u57fa\u6216\u6c28\u57fa\u9178\u4e4b\u95f4\u7684\u5339\u914d\u6216\u4e0d\u5339\u914d\u6765\u5b9a\u4e49\u3002\u4f8b\u5982\u5728DNA\u5e8f\u5217\u6bd4\u5bf9\u4e2d\uff0c\u5339\u914d\u5f97\u5206\u53ef\u4ee5\u8bbe\u4e3a\u6b63\u503c1\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u8fd9\u91cc\u5bf9\u5404\u79cd\u77e9\u9635\u4e0d\u7406\u89e3\u53ef\u4ee5\u76f4\u63a5\u8df3\u8fc7\uff0c\u76f4\u63a5\u4e86\u89e3\u4e0b\u8fb9\u4f8b\u5982\u4e2d\u7684\u4e24\u4e2a\u77e9\u9635\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4f8b\u5982\uff1a\u4e0b\u8fb9\u4e24\u4e2a\u77e9\u9635\uff0c\u7b2c\u4e00\u4e2a\u53ea\u8003\u8651\u5339\u914d\u4e0a\u7684\u5f97\u5206\u4e3a1\uff0c\u7b2c\u4e8c\u4e2a\u53ea\u8003\u8651\u4e86\u8003\u8651\u98a0\u6362\u548c\u7f6e\u6362\u7684\u60c5\u51b5\uff08\u5e8f\u5217\u4e2d\u560c\u5464\u88ab\u5627\u5576\u66ff\u6362\u6216\u53cd\u4e4b\uff0c\u79f0\u4e3a\u98a0\u6362\uff08transversion\uff09\uff0c\u5982A\u2192C\u3001G\u2192T\uff1b\u560c\u5464\u6216\u5627\u5576\u81ea\u5df1\u4e92\u6362\u79f0\u4e3a\u7f6e\u6362\uff08transition\uff09\uff0c\u5982A\u2192G\u3001C\u2192T\u3002\u53ef\u5bf9\u6838\u82f7\u9178\u7684\u98a0\u6362\u4e0e\u7f6e\u6362\u7ed9\u4e88\u4e0d\u540c\u7684\u8bc4\u5206\u3002\uff09\u5728\u5e8f\u5217\u6bd4\u5bf9\u4e2d\u6253\u5206\u77e9\u9635\u53ef\u4ee5\u6839\u636e\u9700\u6c42\u8fdb\u884c\u81ea\u5b9a\u4e49\uff5e<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.0407407407407407\" data-s=\"300,640\" data-type=\"png\" data-w=\"270\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_JB7wiba1sicfeMrO1bOI7uTibpapmA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5gc4XkNAf5SqoNEg2Imv7TVBTGPCJB7wiba1sicfeMrO1bOI7uTibpapmA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4727272727272727\" data-s=\"300,640\" data-type=\"png\" data-w=\"440\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_81ecalz5bno97cRZO5haxp0dvuGy4w.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5A7ibvt98PrGlbswjtvboJFs6Q81ecalz5bno97cRZO5haxp0dvuGy4w\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<h3 style=\"text-align: center;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><em><span style=\"font-size: 20px;\"><strong>\u6bd4\u5bf9\u7b97\u6cd5<\/strong><\/span><\/em><\/span><strong><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:16.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h3>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5168\u5c40\u6bd4\u5bf9\uff1a\u7ed9\u5b9a\u4e24\u4e2a\u5e8f\u5217A\uff1da1a2\u2026an \u548cB\uff1db1b2\u2026bm\uff0cS\uff08i\uff0cj\uff09\u8868\u793a\u4e24\u4e2a\u5e8f\u5217\u6240\u6709\u6bd4\u5bf9\u7684\u6700\u597d\u5206\u6570\u3002\u5728\u8bbe\u5b9a\u521d\u503c\u540e\u53ef\u4ee5\u7528\u4ee5\u4e0b\u9012\u5f52\u5173\u7cfb\u8ba1\u7b97\uff1a<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.21470588235294116\" data-s=\"300,640\" data-type=\"png\" data-w=\"680\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-03_\u5341\u5206\u949f\u5403\u900fblast\u6bd4\u5bf9\u5e95\u5c42\u903b\u8f91\u5e76\u5b9e\u64cd\/6_znLW9J8icETsfBGyqoJjHp17t91x2Q.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q55SetxU2TNWice4JbGs6ZpIV28IznLW9J8icETsfBGyqoJjHp17t91x2Q\/640?wx_fmt=png'\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5c40\u90e8\u6bd4\u5bf9\u7684\u5177\u4f53\u7b97\u6cd5\u4e0e\u5168\u5c40\u6bd4\u5bf9\u51e0\u4e4e\u4e00\u6837\uff0c\u7ed9\u5b9a\u4e24\u4e2a\u5e8f\u5217A\uff1da1a2\u2026an \u548cB\uff1db1b2\u2026bm\uff0cS\uff08i\uff0cj\uff09\u8868\u793a\u4e24\u4e2a\u5e8f\u5217\u4efb\u4f55\u6bd4\u5bf9\u7684\u6700\u597d\u5206\u6570\u3002\u5728\u8bbe\u5b9a\u521d\u503c\u540e\u53ef\u4ee5\u7528\u4ee5\u4e0b\u9012\u5f52\u5173\u7cfb\u8ba1\u7b97\uff1a<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2701908957415565\" data-s=\"300,640\" data-type=\"png\" data-w=\"681\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_KuvtuvsGZqCIlF3IfOLdmdu5IibF1g.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5192FfPialATTCrcB60ev5mX7D8KuvtuvsGZqCIlF3IfOLdmdu5IibF1g\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0a\u8ff0\u7684\u7b97\u6cd5\u4e2d\uff0c\u4e0d\u8bba\u662f\u5168\u5c40\u6bd4\u5bf9\u8fd8\u662f\u5c40\u90e8\u6bd4\u5bf9\u90fd\u662f\u6765\u5bfb\u627e\u6700\u5927\u503c\u7684\u8fc7\u7a0b\uff0c\u6211\u4eec\u9700\u8981\u5f97\u5230Mij\u7684\u5206\u6570\uff0c\u662f\u53d6\u4e86\u4e09\u4e2a\u65b9\u5411\u4e2d\u7684\u6700\u5927\u503c\u3002<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6409691629955947\" data-s=\"300,640\" data-type=\"png\" data-w=\"454\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-03_\u5341\u5206\u949f\u5403\u900fblast\u6bd4\u5bf9\u5e95\u5c42\u903b\u8f91\u5e76\u5b9e\u64cd\/8_aQ0iaNJWEHwfiaPAIHZcUxXHbypzpA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5GA2ouqEpQIbzcEWGibaf1L5FFWibiaQ0iaNJWEHwfiaPAIHZcUxXHbypzpA\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4f8b\u5982\uff1a\u73b0\u5728\u8fd8\u662f\u7528\u5f00\u5934\u65f6\u5019\u6211\u4eec\u7528\u7684\u5e8f\u5217\uff0c\u6211\u4eec\u73b0\u5728\u7528\u52a8\u6001\u89c4\u5212\u7684\u65b9\u5f0f\u6765\u5f97\u5230\u4e0b\u56fe\u4e2d\u7684\u5339\u914d\u65b9\u5f0f\uff1a<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4745098039215686\" data-s=\"300,640\" data-type=\"png\" data-w=\"255\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_SUsIf1XN2ITzUlZlAV8Fy7icKGv9SA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5gjG3iby4cvpBcQjlIMECIfxJ5lSUsIf1XN2ITzUlZlAV8Fy7icKGv9SA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u89c4\u5b9a\u7684\u6253\u5206\u77e9\u9635\u4e3a\uff1a\u5339\u914d\u5f97\u52065\u5206\uff1b\u7a7a\u4f4d\u7f5a\u5206-1\u5206\uff1b\u9519\u914d -3\u5206<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5c0f\u679c\u5efa\u8bae\u53ef\u4ee5\u5728excel\u5c1d\u8bd5\u81ea\u5df1\u7b97\u51fa\u4ee5\u4e0b\u5c0f\u679c\u586b\u5165\u7684\u6570\u5b57\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8fd9\u91cc\u6ce8\u610f\uff0c\u662fmismatch\u8fd8\u662fgap\uff0c\u5982\u679c\u4f60\u662f\u4ece\u5bf9\u89d2\u7ebf\u79fb\u52a8\u7684\u4e3a\u9519\u914d\uff0c\u4ece\u4e0a\u8fb9\u6216\u8005\u53f3\u8fb9\u7684\u683c\u5b50\u79fb\u52a8\u7684\u5373\u4e3agap\uff0c\u8fd9\u4e24\u79cd\u60c5\u51b5\u7684\u7f5a\u5206\u662f\u4e0d\u4e00\u6837\u7684\u3002<\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8934010152284264\" data-s=\"300,640\" data-type=\"png\" data-w=\"591\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-03_\u5341\u5206\u949f\u5403\u900fblast\u6bd4\u5bf9\u5e95\u5c42\u903b\u8f91\u5e76\u5b9e\u64cd\/10_XchqJwDMzDadl0ldEVrIRPOoKib8eA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5OGZQPicdyf4q5INgQWadu6s1LlXchqJwDMzDadl0ldEVrIRPOoKib8eA\/640?wx_fmt=png'\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5f97\u5230\u4e0a\u8ff0\u683c\u5b50\u540e\uff0c\u67e5\u9605\u5230\u5f97\u5206\u6700\u9ad8\u7684\u683c\u5b50\uff0c\u8ffd\u6eaf\u5230\u662f\u600e\u4e48\u6765\u7684\u5e8f\u5217\u5373\u53ef\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<h2 style=\"text-align: center;\"><span style=\"font-size: 20px;\"><em><strong><span style=\"font-family: \u9ed1\u4f53;\"><span style=\"font-family: Arial;\">blast<\/span>\u6bd4\u5bf9\u539f\u7406<\/span><\/strong><\/em><\/span><strong><span style=\"mso-spacerun:'yes';font-family:Arial;mso-fareast-font-family:\u9ed1\u4f53;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:16.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h2>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 10.5pt;\">1\u3001\u7f16\u8bd1\u4e00\u4e2a\u7531\u67e5\u8be2\u5e8f\u5217\u751f\u6210\u7684\u957f\u5ea6\u56fa\u5b9a\u7684\u5b57\u6bb5\u7f16\u8bd1\u5217\u8868\u201cwords\u201d<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 10.5pt;\">2\u3001\u5728\u6570\u636e\u5e93\u4e2d\u626b\u63cf\u83b7\u5f97\u4e0e\u7f16\u8bd1\u5217\u8868\u4e2d\u7684\u5b57\u6bb5\u5339\u914d\u7684\u5e8f\u5217\u8bb0\u5f55\uff1b<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 10.5pt;\">3\u3001\u4ee5\u7f16\u8bd1\u5217\u8868\u4e2d\u7684\u5b57\u6bb5\u5bf9\u4e3a\u4e2d\u5fc3\u5411\u4e24\u7aef\u5ef6\u4f38\u4ee5\u5bfb\u627e\u8d85\u8fc7\u9608\u503c\u5206\u6570S\u7684\u9ad8\u5206\u503c\u7247\u6bb5HSP\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 10.5pt;\">4\u3001\u5bf9\u4e8e\u6bcf\u90e8\u5206HSP\u4f7f\u7528Smith-Watermans\u7b97\u6cd5\u8fdb\u884c\u5c40\u90e8\u6027\u6bd4\u5bf9\uff0c\u4e3a\u6bcf\u90e8\u5206\u6253\u5206\uff0c\u4f5c\u4e3a\u6700\u7ec8\u7ed3\u679c\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 10.5pt;\">\u4e0b\u56fe\uff0c\u662fblast\u7684\u6587\u7ae0\u4e2d\u7684\u539f\u7406<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">E\u503c\u8861\u91cf\u4e86\u5728\u968f\u673a\u60c5\u51b5\u4e0b\uff0c\u6570\u636e\u5e93\u5b58\u5728\u7684\u6bd4\u5f53\u524d\u5339\u914d\u5206\u6570\u66f4\u597d\u7684\u6bd4\u5bf9\u7684\u6570\u76ee\uff0c\u56e0\u6b64\u53ef\u4ee5\u7528\u8be5\u503c\u4f5c\u4e3a\u6307\u6807\u8bc4\u4ef7HSP\u6bd4\u5bf9\u5e8f\u5217\u7684\u53ef\u4fe1\u5ea6\uff0cE\u503c\u8ba1\u7b97\u516c\u5f0f\uff1aE\uff1dKmne\uff0d\u03bbS<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7601572739187418\" data-s=\"300,640\" data-type=\"png\" data-w=\"763\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_AEq0pzTKibrkB4BEgk4MLEkpaYyNMg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5Y19FFE2VBia5A1gCG6QJCoUerCAEq0pzTKibrkB4BEgk4MLEkpaYyNMg\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 10.5pt;\">\u4e86\u89e3\u4e86blast\u7684\u539f\u7406\u540e\uff0c\u4e0b\u9762\u4ecb\u7ecd\u4e00\u4e9bblast\u7684\u5e94\u7528\u548c\u53c2\u6570\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 10.5pt;\"><br \/><\/span><\/p>\n<h2 style=\"text-align: center;\"><em><span style=\"font-size: 20px;\"><strong><span style=\"font-family: \u9ed1\u4f53;\"><span style=\"font-family: Arial;\">blast<\/span>\u6bd4\u5bf9\u5b9e\u9645\u64cd\u4f5c<\/span><\/strong><\/span><\/em><strong><span style=\"mso-spacerun:'yes';font-family:\u9ed1\u4f53;mso-ascii-font-family:Arial;mso-hansi-font-family:Arial;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:16.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h2>\n<h3><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\"><strong>\u6bd4\u5bf9<\/strong><strong>\uff1a<\/strong><\/span><\/h3>\n<section style=\"\">\n<section>\n<section style=\"margin-bottom: 0px;padding-right: 15px;padding-left: 15px;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 0.578px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\">blastn -query input.fasta -db database -out output.txt -evalue 0.001 -outfmt 6<\/span><\/code><code><span class=\"code-snippet_outer\">blastn -query input.fasta -db database -out output.txt -outfmt \" 7 qaccver saccver pident length mismatch qlen qstart qend sstart send qseq evalue bitscore\" -num_threads 10 -word_size#\u4e5f\u53ef\u4ee5\u5c1d\u8bd57\u7684\u683c\u5f0f\u3002<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.11728395061728394\" data-s=\"300,640\" data-type=\"png\" data-w=\"810\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-03_\u5341\u5206\u949f\u5403\u900fblast\u6bd4\u5bf9\u5e95\u5c42\u903b\u8f91\u5e76\u5b9e\u64cd\/12_ZiaRqUWqj14JgullI30bBYPR8hyHyg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5yqZa7l3iaG1t8tSwB7kIO5HI1TZiaRqUWqj14JgullI30bBYPR8hyHyg\/640?wx_fmt=png'\" \/><\/p>\n<section>\n<section>\n<section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u5728\u8fd9\u4e2a\u793a\u4f8b\u4e2d\uff0cblastn\u662fBLAST\u7a0b\u5e8f\u7684\u540d\u79f0\uff0cinput.fasta\u662f\u8f93\u5165\u6587\u4ef6\uff0cdatabase\u662fBLAST\u6570\u636e\u5e93\uff0coutput.txt\u662f\u8f93\u51fa\u7ed3\u679c\u6587\u4ef6\uff0c-evalue\u53c2\u6570\u6307\u5b9a\u8fc7\u6ee4\u7ed3\u679c\u7684\u671f\u671b\u503c\u9608\u503c\uff0c-outfmt\u6307\u5b9a\u8f93\u51fa\u683c\u5f0f\u4e3a\u8868\u683c\u683c\u5f0f\uff086\uff09<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<h3><span style=\"font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>\u6784\u5efa\u6570\u636e\u5e93<\/strong><strong>\uff1a<\/strong><\/span><\/h3>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;\">\n<section>\n<section style=\"padding-right: 15px;padding-left: 15px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\">makeblastdb -dbtype nucl -in ${name}.fa -out ${name}<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5b8c\u6210\u540e\u4f1a\u663e\u793a\u4ee5\u4e0b\u63d0\u793a<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2698412698412698\" data-s=\"300,640\" data-type=\"png\" data-w=\"693\" style=\"height: auto !important;\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-10-03_\u5341\u5206\u949f\u5403\u900fblast\u6bd4\u5bf9\u5e95\u5c42\u903b\u8f91\u5e76\u5b9e\u64cd\/13_DNJmuwME8iampibgwK4Y5ErNWvbnZw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q55Gt5DSIt6BQpbZsr8WicrymrLyPDNJmuwME8iampibgwK4Y5ErNWvbnZw\/640?wx_fmt=png'\" \/><\/p>\n<section style=\"margin-bottom: 0px;\">\n<section>\n<section style=\"padding-right: 15px;padding-left: 15px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);font-size: 14px;visibility: visible;\">\n<section style=\"padding-right: 15px;padding-left: 15px;outline: 0px;visibility: visible;\">\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code><span class=\"code-snippet_outer\">#\u4f1a\u751f\u6210\u4ee5\u4e0b\u7684\u51e0\u4e2a\u6587\u4ef6<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"letter-spacing: 0.578px;text-wrap: wrap;text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5174537987679672\" data-s=\"300,640\" data-type=\"png\" data-w=\"487\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/14_aDZ87Adp7vuAn6WwuTKE3Jj0hRYGWQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5VbF3aXf0hN8dVUTWWxHKDl34aDZ87Adp7vuAn6WwuTKE3Jj0hRYGWQ\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"margin-left: 22pt;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><\/span><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"margin-left:21.0000pt;mso-para-margin-left:2.0000gd;\"><br \/><\/p>\n<h3 style=\"text-align: center;\"><span style=\"font-size: 20px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><em><strong>\u53c2\u6570\u89e3\u8bfb<\/strong><strong>\uff1a<\/strong><\/em><\/span><strong><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:16.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h3>\n<p style=\"margin-left: 22pt;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">1\u3001E-value\uff08\u671f\u671b\u503c\uff09\uff1a<\/span><\/strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">E-value\u662f\u4e00\u4e2a\u8861\u91cf\u6bd4\u5bf9\u7ed3\u679c\u7684\u671f\u671b\u968f\u673a\u53d1\u751f\u6b21\u6570\u7684\u6307\u6807\u3002\u5b83\u8868\u793a\u5728\u8fdb\u884c\u5927\u91cf\u968f\u673a\u6bd4\u5bf9\u7684\u60c5\u51b5\u4e0b\uff0c\u671f\u671b\u5f97\u5230\u4e0e\u67e5\u8be2\u5e8f\u5217\u7684\u6bd4\u5bf9\u76f8\u4f3c\u6027\u81f3\u5c11\u4e00\u6837\u597d\uff08\u6216\u66f4\u597d\uff09\u7684\u7ed3\u679c\u7684\u6b21\u6570\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u8f83\u5c0f\u7684E-value\u8868\u793a\u6bd4\u5bf9\u7ed3\u679c\u7684\u76f8\u4f3c\u6027\u66f4\u663e\u8457\u548c\u663e\u8457\u3002\u901a\u5e38\uff0cE-value\u5c0f\u4e8e\u67d0\u4e2a\u9884\u8bbe\u7684\u9608\u503c\uff08\u4f8b\u59820.001\u62160.05\uff09\u88ab\u8ba4\u4e3a\u662f\u663e\u8457\u7684\u6bd4\u5bf9\u7ed3\u679c\u3002\u5728BLAST\u8f93\u51fa\u4e2d\uff0cE-value\u4e00\u822c\u4ee5\u79d1\u5b66\u8ba1\u6570\u6cd5\u7684\u5f62\u5f0f\u7ed9\u51fa\uff0c\u4f8b\u59821.0e-10\uff0c\u8868\u793a10\u7684\u8d1f10\u6b21\u65b9\uff08\u53730.0000000001\uff09\u3002<o:p><\/o:p><\/span><\/p>\n<p><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">2\u3001Score\u662f\u57fa\u4e8e\u6bd4\u5bf9\u4e2d\u7684\u5339\u914d\u548c\u4e0d\u5339\u914d\u8fdb\u884c\u8ba1\u7b97\u7684\u4e00\u4e2a\u503c<\/span><\/strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\uff0c\u7528\u4e8e\u8861\u91cf\u6bd4\u5bf9\u7684\u8d28\u91cf\u548c\u76f8\u4f3c\u6027\u3002\u5177\u4f53\u7684\u5f97\u5206\u8ba1\u7b97\u65b9\u5f0f\u53d6\u51b3\u4e8e\u6240\u4f7f\u7528\u7684\u6253\u5206\u77e9\u9635\u3002\u4e00\u822c\u6765\u8bf4\uff0c\u8f83\u9ad8\u7684Score\u8868\u793a\u6bd4\u5bf9\u7ed3\u679c\u5177\u6709\u66f4\u597d\u7684\u76f8\u4f3c\u6027\u548c\u5339\u914d\u7a0b\u5ea6\u3002\u901a\u5e38\u60c5\u51b5\u4e0b\uff0cScore\u8d8a\u9ad8\uff0c\u6bd4\u5bf9\u7684\u8d28\u91cf\u8d8a\u597d\u3002\u5728BLAST\u8f93\u51fa\u4e2d\uff0cScore\u662f\u4ee5\u5177\u4f53\u6570\u503c\u7684\u5f62\u5f0f\u7ed9\u51fa\u3002<o:p><\/o:p><\/span><\/p>\n<p><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">3\u3001\u201cIdentity\u201d\uff08\u76f8\u540c\u6027\uff09\u662f\u8f93\u51fa\u7ed3\u679c\u4e2d\u7684\u4e00\u4e2a\u91cd\u8981\u6307\u6807<\/span><\/strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\uff0c\u7528\u4e8e\u63cf\u8ff0\u6bd4\u5bf9\u5e8f\u5217\u7684\u76f8\u4f3c\u6027\u7a0b\u5ea6\u3002\u5b83\u8868\u793a\u5728\u6bd4\u5bf9\u8fc7\u7a0b\u4e2d\uff0c\u4e24\u4e2a\u5e8f\u5217\u4e2d\u5b58\u5728\u76f8\u540c\u7684\u78b1\u57fa\u6216\u6c28\u57fa\u9178\u7684\u767e\u5206\u6bd4\u3002\u76f8\u540c\u6027\uff08Identity\uff09\uff1a\u5728\u6bd4\u5bf9\u7ed3\u679c\u4e2d\uff0c\u76f8\u540c\u6027\u901a\u5e38\u4ee5\u767e\u5206\u6bd4\u7684\u5f62\u5f0f\u8868\u793a\uff0c\u53cd\u6620\u4e86\u4e24\u4e2a\u5e8f\u5217\u4e2d\u76f8\u540c\u4f4d\u7f6e\u7684\u78b1\u57fa\u6216\u6c28\u57fa\u9178\u7684\u767e\u5206\u6bd4\u3002\u8f83\u9ad8\u7684\u76f8\u540c\u6027\u8868\u793a\u6bd4\u5bf9\u7ed3\u679c\u5177\u6709\u66f4\u9ad8\u7684\u76f8\u4f3c\u6027\u3002\u76f8\u540c\u6027\u7684\u8ba1\u7b97\uff1a\u76f8\u540c\u6027\u7684\u8ba1\u7b97\u662f\u901a\u8fc7\u6bd4\u5bf9\u8fc7\u7a0b\u4e2d\u7684\u5339\u914d\uff08Matches\uff09\u548c\u4e0d\u5339\u914d\uff08Mismatches\uff09\u6570\u91cf\u6765\u5f97\u51fa\u7684\u3002\u8ba1\u7b97\u516c\u5f0f\uff1aIdentity\uff08\u76f8\u540c\u6027\uff09= (Matches \/ Alignment Length) \u00d7 100%<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5176\u4e2d\uff0cMatches\u8868\u793a\u6bd4\u5bf9\u4e2d\u5b8c\u5168\u5339\u914d\u7684\u4f4d\u7f6e\u6570\uff0cAlignment Length\u8868\u793a\u6bd4\u5bf9\u7684\u5e8f\u5217\u957f\u5ea6\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4f8b\u5982\uff0c\u5047\u8bbe\u6709\u4e00\u4e2a\u6bd4\u5bf9\u7ed3\u679c\uff0c\u5e8f\u5217\u7684\u957f\u5ea6\u4e3a100\uff0c\u5176\u4e2d\u670980\u4e2a\u4f4d\u7f6e\u662f\u5b8c\u5168\u5339\u914d\u7684\uff0c\u90a3\u4e48\u76f8\u540c\u6027\u4e3a\uff1a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">Identity = (80 \/ 100) \u00d7 100% = 80%<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u8bf7\u6ce8\u610f\uff0c\u76f8\u540c\u6027\u53ea\u8003\u8651\u4e86\u5b8c\u5168\u5339\u914d\u7684\u4f4d\u7f6e\uff0c\u4e0d\u8003\u8651\u7f3a\u5931\uff08Gaps\uff09\u548c\u63d2\u5165\uff08Insertions\uff09\u3002\u7f3a\u5931\u548c\u63d2\u5165\u4f1a\u5f71\u54cd\u76f8\u4f3c\u6027\u548c\u6bd4\u5bf9\u8d28\u91cf\u7684\u5176\u4ed6\u65b9\u9762\uff0c\u4f8b\u5982Gap Coverage\u548cGap Length\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">Identity\u3001E-value\u3001Score\u3001Coverage\u7b49\u3002\u8fd9\u4e9b\u6307\u6807\u7684\u7efc\u5408\u5206\u6790\u53ef\u4ee5\u63d0\u4f9b\u66f4\u5168\u9762\u7684\u4e86\u89e3\u548c\u89e3\u91ca\u6bd4\u5bf9\u7ed3\u679c\u7684\u76f8\u4f3c\u6027\u548c\u53ef\u9760\u6027\u3002<o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<p><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5c0f\u679c\u9644\u4e0ablasn\u547d\u4ee4\u7684\u8be6\u7ec6\u89e3\u91ca\uff1a<\/span><\/strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">-query <filename>\uff1a\u6307\u5b9a\u8f93\u5165\u6587\u4ef6\uff0c\u5176\u4e2d\u5305\u542b\u5f85\u6bd4\u5bf9\u7684\u67e5\u8be2\u5e8f\u5217\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">-db <database_name>\uff1a\u6307\u5b9a\u6bd4\u5bf9\u6240\u4f7f\u7528\u7684\u6570\u636e\u5e93\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">-out <output_file>\uff1a\u6307\u5b9a\u8f93\u51fa\u6587\u4ef6\u7684\u540d\u79f0\u6216\u8def\u5f84\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">-evalue 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