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14px;\">\u5355\u7ec6\u80deRNA-seq\u6280\u672f\u80fd\u591f\u72ec\u7acb\u5730\u68c0\u6d4b\u6bcf\u4e2a\u7ec6\u80de\u7684RNA\u8868\u8fbe\u60c5\u51b5\uff0c\u5373\u4f7f\u662f\u9057\u4f20\u4e0a\u76f8\u540c\u7684\u7ec6\u80de\uff0c\u5728\u76f8\u540c\u73af\u5883\u4e0b\u4e5f\u53ef\u80fd\u8868\u73b0\u51fa\u4e0d\u540c\u7684\u57fa\u56e0\u548c\u86cb\u767d\u8d28\u8868\u8fbe\u6c34\u5e73\uff0c\u8fd9\u53ef\u80fd\u5bfc\u81f4\u4e00\u4e9b\u7ec6\u80de\u4ea7\u751f\u6297\u836f\u6027\u3002\u5355\u7ec6\u80deRNA-seq\u6280\u672f\u5c31\u53ef\u4ee5\u53d1\u73b0\u8fd9\u4e9b\u7a00\u6709\u7684\u53d8\u5f02\u7ec6\u80de\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.36462093862815886\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-07-07_\u5355\u7ec6\u80de\u6d41\u7a0b\u6559\u7a0b\uff08\u4e00\uff09\uff1a\u4ec0\u4e48\u662f\u5355\u7ec6\u80de\/2_A97gicMQ2T9CicMvbmaDiaJgtRoJgQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxyhIvUcmyfxPcia7Q2vbZvCKjFs0UN886QILWGjfMzk97yrdhLA97gicMQ2T9CicMvbmaDiaJgtRoJgQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\"><\/p>\n<p style=\"white-space: normal;text-align: center;\"><span style=\"font-size: 12px;\">\u5b9e\u9a8c\u65b9\u6cd5<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5728\u4ee5\u5f80\u7684RNA-seq\u4e2d\uff0c\u5e38\u4f7f\u7528bulk RNA-seq\u8fdb\u884c\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u8be5\u65b9\u6cd5\u53ef\u4ee5\u63a2\u7d22\u4e0d\u540c\u6761\u4ef6\u4e4b\u95f4\u57fa\u56e0\u8868\u8fbe\u7684\u5dee\u5f02\uff08\u6b63\u5e38\u7ec4\u548c\u5bf9\u7167\u7ec4\uff09\uff0c\u4f46\u662f\u65e0\u6cd5\u6355\u6349\u7ec6\u80de\u6c34\u5e73\u7684\u5dee\u5f02\uff0c\u5982\u4e0b\u56fe\u6240\u793a\uff0cbulk RNA-seq\u65e0\u6cd5\u6b63\u786e\u7684\u68c0\u6d4bgeneA\u548cgeneB\u4e4b\u95f4\u7684\u76f8\u5173\u6027\uff0c\u800cscRNA-seq\u5219\u53ef\u4ee5\u6b63\u786e\u7684\u5bf9\u7ec6\u80de\u8fdb\u884c\u5206\u7ec4\uff0c\u68c0\u6d4bgeneA\u4e0egeneB\u4e4b\u95f4\u7684\u5173\u8054\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5042735042735043\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_rOMX978IL1LicDNtyTuzYic6Hk0MBA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxyhIvUcmyfxPcia7Q2vbZvCw7gOZKU4wz02CVKc21eZsl4AQGrOMX978IL1LicDNtyTuzYic6Hk0MBA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"468\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u867d\u7136scRNA-seq\u80fd\u6355\u83b7\u7ec6\u80de\u6c34\u5e73\u7684\u8868\u8fbe\uff0c\u4f46\u662f\u4e5f\u5177\u6709\u4ee5\u4e0b\u7f3a\u70b9\uff1a<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">1\u3001 \u6570\u636e\u91cf\u5927<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">ScRNA-seq\u9700\u8981\u6355\u6349\u5355\u4e2a\u7ec6\u80de\u7684\u8868\u8fbe\uff0c\u76f8\u8f83\u4e8e\u5e38\u89c4RNA-seq\u6570\u636e\u91cf\u5927\u5f97\u591a\uff0c\u9700\u8981\u66f4\u591a\u7b97\u529b\u3001\u5185\u5b58\u3001\u5b58\u50a8\u7a7a\u95f4\u3001\u65f6\u95f4\u6765\u8fdb\u884c\u5206\u6790<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">2\u3001 \u5355\u4e2a\u7ec6\u80de\u6d4b\u5e8f\u6df1\u5ea6\u8f83\u4f4e<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u7531\u4e8escRNA-seq\u4f7f\u7528\u7684\u6db2\u6ef4\u6d4b\u5e8f\u6cd5\uff0c\u5176\u6d4b\u5e8f\u6df1\u5ea6\u8f83\u4f4e\uff0c\u901a\u5e38\u5355\u4e2a\u7ec6\u80de\u7684\u8986\u76d6\u7387\u4ec510%\u81f350%\u3002\u8fd9\u53ef\u80fd\u5bfc\u81f4\u5927\u91cf\u57fa\u56e0\u5728\u7ec6\u80de\u4e2d\u68c0\u6d4b\u4e0d\u5230\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">3\u3001 \u6837\u54c1\u7684\u4e0d\u53ef\u63a7\u6027<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">Biological variation\u53ef\u80fd\u5bfc\u81f4\u7ec6\u80de\u4e4b\u95f4\u7684\u57fa\u56e0\u8868\u8fbe\u4e0e\u5b9e\u9645\u7684\u751f\u7269\u7ec6\u80de\u7c7b\u578b\/\u72b6\u6001\u66f4\u76f8\u4f3c\u6216\u4e0d\u540c\uff0c\u8fd9\u53ef\u80fd\u4f1a\u63a9\u76d6\u7ec6\u80de\u7c7b\u578b\u7684\u8eab\u4efd\u3002biological variation\u7684\u6765\u6e90\u5305\u62ec\uff1a<\/span><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"font-size: 14px;\">Transcriptional bursting\uff1a<\/span><\/strong><span style=\"font-size: 14px;\">\u5e76\u975e\u6240\u6709\u57fa\u56e0\u90fd\u5728\u6301\u7eed\u8f6c\u5f55\u3002\u7ec6\u80de\u7684\u91c7\u96c6\u65f6\u95f4\u5c06\u51b3\u5b9a\u6bcf\u4e2a\u7ec6\u80de\u4e2d\u57fa\u56e0\u662f\u8868\u8fbe\u8fd8\u662f\u6c89\u9ed8\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"font-size: 14px;\">Varying rates of RNA processing\uff1a<\/span><\/strong><span style=\"font-size: 14px;\">\u4e0d\u540c\u7684RNA\u52a0\u5de5\u7684\u901f\u5ea6\u4e0d\u540c\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"font-size: 14px;\">Temporal changes\uff1a<\/span><\/strong><span style=\"font-size: 14px;\">\u4e0d\u65ad\u53d8\u5316\u7684\u7ec6\u80de\u8fc7\u7a0b\uff0c\u5982\u7ec6\u80de\u5468\u671f\uff0c\u4f1a\u5f71\u54cd\u5355\u4e2a\u7ec6\u80de\u7684\u57fa\u56e0\u8868\u8fbe\u8c31\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5c3d\u7ba1scRNA-seq\u5b58\u5728\u5404\u79cd\u95ee\u9898\uff0c\u4f46\u5b83\u4ecd\u662f\u4e00\u79cd\u529f\u80fd\u5f3a\u5927\u4e14\u5177\u6709\u8f83\u9ad8\u5206\u8fa8\u7387\u7684\u65b9\u6cd5\uff0c\u9002\u5408\u7528\u4e8e\u5206\u6790\u5355\u7ec6\u80de\u6c34\u5e73\u7684\u57fa\u56e0\u8868\u8fbe\u3002\u9488\u5bf9scRNA-seq\u7684\u5404\u79cd\u95ee\u9898\uff0c\u4e5f\u6f14\u5316\u51fa\u4e86\u5355\u7ec6\u80de\u6838\u6d4b\u5e8f\u7b49\u65b9\u6cd5\u3002\u603b\u800c\u8a00\u4e4b\uff0cscRNA-seq\u662f\u4e00\u9879\u6781\u5177\u524d\u666f\u7684\u5355\u7ec6\u80de\u6c34\u5e73\u7684\u6d4b\u5e8f\u6280\u672f\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<section style=\"margin-bottom: 0px;padding-right: 1em;padding-left: 1em;font-size: 16px;letter-spacing: 0.578px;text-align: center;white-space: normal;caret-color: rgba(0, 0, 0, 0);display: inline-block;\"><span title=\"\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:9.title1\" style=\"padding: 0.3em 0.5em;display: inline-block;border-radius: 0.5em;font-size: 14px;color: rgb(255, 255, 255);background-color: rgb(71, 193, 168);\"><\/p>\n<p>\u5f80\u671f\u63a8\u8350<br \/><\/p>\n<p><\/span><\/section>\n<p><br \/><\/p>\n<section style=\"margin-top: -1em;margin-bottom: 0px;padding: 20px 10px 10px;font-size: 16px;letter-spacing: 0.578px;text-align: center;white-space: normal;caret-color: rgba(0, 0, 0, 0);border-width: 1px;border-style: solid;border-color: rgb(192, 200, 209);background-color: rgb(239, 239, 239);\">\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<p><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488460&idx=1&sn=3069e8e7f85558703a8ae813375c1552&chksm=cf7c8309f80b0a1fd21e59e711c31141c96f126290f21bf78e2103d5716bdbf41acc36d6ca2a&scene=21#wechat_redirect\" textvalue=\"1\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\"><span style=\"font-size: 14px;\">1<\/span><\/a><span style=\"font-size: 14px;\"> <\/span><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488460&idx=1&sn=3069e8e7f85558703a8ae813375c1552&chksm=cf7c8309f80b0a1fd21e59e711c31141c96f126290f21bf78e2103d5716bdbf41acc36d6ca2a&scene=21#wechat_redirect\" textvalue=\"PCA\u7ed8\u56fe\uff0c\u4ece\u539f\u7406\u5230\u7ed8\u56fe\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\">PCA\u7ed8\u56fe\uff0c\u4ece\u539f\u7406\u5230\u7ed8\u56fe<\/a><br \/><\/p>\n<p><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488459&idx=1&sn=d9a5d7f588994d2602cc1826c2e4f2e5&chksm=cf7c830ef80b0a1883f47601bd39bf81120fc16bc0d02f150a83f40ce52d29fbec17458830f1&scene=21#wechat_redirect\" textvalue=\"\u5c0f\u679c\u7684\u5355\u65e5\u5c0f\u6280\u5de7DAY6\uff1a\u5982\u4f55\u5229\u7528panImmune\u8f7b\u677e\u5206\u6790\u514d\u75ab\u6d78\u6da6\uff1f\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\">2 \u5c0f\u679c\u7684\u5355\u65e5\u5c0f\u6280\u5de7DAY6\uff1a\u5982\u4f55\u5229\u7528panImmune\u8f7b\u677e\u5206\u6790\u514d\u75ab\u6d78\u6da6\uff1f<\/a><br \/><\/p>\n<p><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488458&idx=1&sn=62bd0f5561698111f46d66c681d0eaf8&chksm=cf7c830ff80b0a19e2577c9c70b473076416343b70fbc563d423257619d5abe7104ffa5ea158&scene=21#wechat_redirect\" textvalue=\"3\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\"><span style=\"font-size: 14px;\">3<\/span><\/a><span style=\"font-size: 14px;\"> <\/span><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488458&idx=1&sn=62bd0f5561698111f46d66c681d0eaf8&chksm=cf7c830ff80b0a19e2577c9c70b473076416343b70fbc563d423257619d5abe7104ffa5ea158&scene=21#wechat_redirect\" textvalue=\"Fastp\u8f6f\u4ef6\uff1a\u5904\u7406fastq\u6587\u4ef6\u5b83\u8d85\u68d2\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\">Fastp\u8f6f\u4ef6\uff1a\u5904\u7406fastq\u6587\u4ef6\u5b83\u8d85\u68d2\uff01<\/a><br \/><\/p>\n<p><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488440&idx=1&sn=cf2e3e91652b4c8738ec8b092ede68b6&chksm=cf7c837df80b0a6b09221a3239345182772097dff21e23b36e5ed0f73b08d8c9f2e496c2f3e8&scene=21#wechat_redirect\" textvalue=\"\u9ad8\u5206\u751f\u4fe1SCI\u5e38\u7528\u7684\u80bf\u7624\u514d\u75ab\u5b66\u5355\u7ec6\u80de\u5206\u6790\u5de5\u5177\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\">4 \u9ad8\u5206\u751f\u4fe1SCI\u5e38\u7528\u7684\u80bf\u7624\u514d\u75ab\u5b66\u5355\u7ec6\u80de\u5206\u6790\u5de5\u5177<\/a><br \/><\/p>\n<p><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488459&idx=2&sn=5856888f0c879f1d2989c47da08cb064&chksm=cf7c830ef80b0a1865b8bc9efd54d6726e1b34a3f4b44bf312c19e3f1c0c38eacd6428bb6897&scene=21#wechat_redirect\" textvalue=\"\u57fa\u56e0\u7ef4\u5ea6\u592a\u9ad8\u600e\u4e48\u529e\uff1f\u65e0\u6240\u8c13\uff01PCA\u964d\u7ef4\u4f1a\u51fa\u624b\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"11\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\">5 \u57fa\u56e0\u7ef4\u5ea6\u592a\u9ad8\u600e\u4e48\u529e\uff1f\u65e0\u6240\u8c13\uff01PCA\u964d\u7ef4\u4f1a\u51fa\u624b\uff01<\/a><\/p>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"578\" data-cropsely1=\"0\" data-cropsely2=\"322\" data-galleryid=\"\" data-ratio=\"0.7193195625759417\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-07-07_\u5355\u7ec6\u80de\u6d41\u7a0b\u6559\u7a0b\uff08\u4e00\uff09\uff1a\u4ec0\u4e48\u662f\u5355\u7ec6\u80de\/4_oRxs73HBHUoxCtAqBPQnq1AXricRvg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxenwVZiaibuOTpj6MMDUj2LoPdS6tic40bbGQV7QyM6QOQY8EFoRxs73HBHUoxCtAqBPQnq1AXricRvg\/640?wx_fmt=png'\" data-type=\"jpeg\" data-w=\"823\" style=\"width: 578px;height: 416px;\"><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;font-size: 16px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 <\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/mp.weixin.qq.com\/s\/sfk0hRc6tkxjkKkvuVHjnA#rd\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/mp.weixin.qq.com\/s\/sfk0hRc6tkxjkKkvuVHjnA#rd\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br 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14px;\">\u9996\u5148\u8ddf\u7740\u5c0f\u679c\u6765\u5b66\u4e60scRNA\u7684\u7528\u9014\u3002\u6211\u4eec\u751f\u7269\u72d7\u4ee5\u524d\u901a\u5e38\u4f7f\u7528RNA\u6d4b\u5e8f\uff08RNA-seq\uff09\u6765\u68c0\u6d4b\u6837\u672c\u4e2d\u7684\u6240\u6709RNA\u8f6c\u5f55\u672c\uff0c\u5206\u6790\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\u3002\u4f46\u662f\u95ee\u9898\u662f\uff0c\u8fd9\u79cd\u6d4b\u5e8f\u901a\u5e38\u662f\u5bf9\u7ec4\u7ec7\u6837\u672c\u6216\u8005\u7ec6\u80de\u7fa4\u8fdb\u884c\u7684\uff0c\u8fd9\u6837\u53ef\u80fd\u63a9\u76d6\u4e86\u4e0d\u540c\u7ec6\u80de\u4e4b\u95f4\u7684\u5dee\u5f02\u3002<\/span><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5355\u7ec6\u80deRNA-seq\u6280\u672f\u80fd\u591f\u72ec\u7acb\u5730\u68c0\u6d4b\u6bcf\u4e2a\u7ec6\u80de\u7684RNA\u8868\u8fbe\u60c5\u51b5\uff0c\u5373\u4f7f\u662f\u9057\u4f20\u4e0a\u76f8\u540c\u7684\u7ec6\u80de\uff0c\u5728\u76f8\u540c\u73af\u5883\u4e0b\u4e5f\u53ef\u80fd\u8868\u73b0\u51fa\u4e0d\u540c\u7684\u57fa\u56e0\u548c\u86cb\u767d\u8d28\u8868\u8fbe\u6c34\u5e73\uff0c\u8fd9\u53ef\u80fd\u5bfc\u81f4\u4e00\u4e9b\u7ec6\u80de\u4ea7\u751f\u6297\u836f\u6027\u3002\u5355\u7ec6\u80deRNA-seq\u6280\u672f\u5c31\u53ef\u4ee5\u53d1\u73b0\u8fd9\u4e9b\u7a00\u6709\u7684\u53d8\u5f02\u7ec6\u80de\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.36462093862815886\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-07-07_\u5355\u7ec6\u80de\u6d41\u7a0b\u6559\u7a0b\uff08\u4e00\uff09\uff1a\u4ec0\u4e48\u662f\u5355\u7ec6\u80de\/2_A97gicMQ2T9CicMvbmaDiaJgtRoJgQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxyhIvUcmyfxPcia7Q2vbZvCKjFs0UN886QILWGjfMzk97yrdhLA97gicMQ2T9CicMvbmaDiaJgtRoJgQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\"><\/p>\n<p style=\"white-space: normal;text-align: center;\"><span style=\"font-size: 12px;\">\u5b9e\u9a8c\u65b9\u6cd5<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5728\u4ee5\u5f80\u7684RNA-seq\u4e2d\uff0c\u5e38\u4f7f\u7528bulk RNA-seq\u8fdb\u884c\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u8be5\u65b9\u6cd5\u53ef\u4ee5\u63a2\u7d22\u4e0d\u540c\u6761\u4ef6\u4e4b\u95f4\u57fa\u56e0\u8868\u8fbe\u7684\u5dee\u5f02\uff08\u6b63\u5e38\u7ec4\u548c\u5bf9\u7167\u7ec4\uff09\uff0c\u4f46\u662f\u65e0\u6cd5\u6355\u6349\u7ec6\u80de\u6c34\u5e73\u7684\u5dee\u5f02\uff0c\u5982\u4e0b\u56fe\u6240\u793a\uff0cbulk RNA-seq\u65e0\u6cd5\u6b63\u786e\u7684\u68c0\u6d4bgeneA\u548cgeneB\u4e4b\u95f4\u7684\u76f8\u5173\u6027\uff0c\u800cscRNA-seq\u5219\u53ef\u4ee5\u6b63\u786e\u7684\u5bf9\u7ec6\u80de\u8fdb\u884c\u5206\u7ec4\uff0c\u68c0\u6d4bgeneA\u4e0egeneB\u4e4b\u95f4\u7684\u5173\u8054\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5042735042735043\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_rOMX978IL1LicDNtyTuzYic6Hk0MBA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxyhIvUcmyfxPcia7Q2vbZvCw7gOZKU4wz02CVKc21eZsl4AQGrOMX978IL1LicDNtyTuzYic6Hk0MBA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"468\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u867d\u7136scRNA-seq\u80fd\u6355\u83b7\u7ec6\u80de\u6c34\u5e73\u7684\u8868\u8fbe\uff0c\u4f46\u662f\u4e5f\u5177\u6709\u4ee5\u4e0b\u7f3a\u70b9\uff1a<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">1\u3001 \u6570\u636e\u91cf\u5927<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">ScRNA-seq\u9700\u8981\u6355\u6349\u5355\u4e2a\u7ec6\u80de\u7684\u8868\u8fbe\uff0c\u76f8\u8f83\u4e8e\u5e38\u89c4RNA-seq\u6570\u636e\u91cf\u5927\u5f97\u591a\uff0c\u9700\u8981\u66f4\u591a\u7b97\u529b\u3001\u5185\u5b58\u3001\u5b58\u50a8\u7a7a\u95f4\u3001\u65f6\u95f4\u6765\u8fdb\u884c\u5206\u6790<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">2\u3001 \u5355\u4e2a\u7ec6\u80de\u6d4b\u5e8f\u6df1\u5ea6\u8f83\u4f4e<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u7531\u4e8escRNA-seq\u4f7f\u7528\u7684\u6db2\u6ef4\u6d4b\u5e8f\u6cd5\uff0c\u5176\u6d4b\u5e8f\u6df1\u5ea6\u8f83\u4f4e\uff0c\u901a\u5e38\u5355\u4e2a\u7ec6\u80de\u7684\u8986\u76d6\u7387\u4ec510%\u81f350%\u3002\u8fd9\u53ef\u80fd\u5bfc\u81f4\u5927\u91cf\u57fa\u56e0\u5728\u7ec6\u80de\u4e2d\u68c0\u6d4b\u4e0d\u5230\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">3\u3001 \u6837\u54c1\u7684\u4e0d\u53ef\u63a7\u6027<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">Biological variation\u53ef\u80fd\u5bfc\u81f4\u7ec6\u80de\u4e4b\u95f4\u7684\u57fa\u56e0\u8868\u8fbe\u4e0e\u5b9e\u9645\u7684\u751f\u7269\u7ec6\u80de\u7c7b\u578b\/\u72b6\u6001\u66f4\u76f8\u4f3c\u6216\u4e0d\u540c\uff0c\u8fd9\u53ef\u80fd\u4f1a\u63a9\u76d6\u7ec6\u80de\u7c7b\u578b\u7684\u8eab\u4efd\u3002biological variation\u7684\u6765\u6e90\u5305\u62ec\uff1a<\/span><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"font-size: 14px;\">Transcriptional bursting\uff1a<\/span><\/strong><span style=\"font-size: 14px;\">\u5e76\u975e\u6240\u6709\u57fa\u56e0\u90fd\u5728\u6301\u7eed\u8f6c\u5f55\u3002\u7ec6\u80de\u7684\u91c7\u96c6\u65f6\u95f4\u5c06\u51b3\u5b9a\u6bcf\u4e2a\u7ec6\u80de\u4e2d\u57fa\u56e0\u662f\u8868\u8fbe\u8fd8\u662f\u6c89\u9ed8\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"font-size: 14px;\">Varying rates of RNA processing\uff1a<\/span><\/strong><span style=\"font-size: 14px;\">\u4e0d\u540c\u7684RNA\u52a0\u5de5\u7684\u901f\u5ea6\u4e0d\u540c\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><strong><span style=\"font-size: 14px;\">Temporal changes\uff1a<\/span><\/strong><span style=\"font-size: 14px;\">\u4e0d\u65ad\u53d8\u5316\u7684\u7ec6\u80de\u8fc7\u7a0b\uff0c\u5982\u7ec6\u80de\u5468\u671f\uff0c\u4f1a\u5f71\u54cd\u5355\u4e2a\u7ec6\u80de\u7684\u57fa\u56e0\u8868\u8fbe\u8c31\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5c3d\u7ba1scRNA-seq\u5b58\u5728\u5404\u79cd\u95ee\u9898\uff0c\u4f46\u5b83\u4ecd\u662f\u4e00\u79cd\u529f\u80fd\u5f3a\u5927\u4e14\u5177\u6709\u8f83\u9ad8\u5206\u8fa8\u7387\u7684\u65b9\u6cd5\uff0c\u9002\u5408\u7528\u4e8e\u5206\u6790\u5355\u7ec6\u80de\u6c34\u5e73\u7684\u57fa\u56e0\u8868\u8fbe\u3002\u9488\u5bf9scRNA-seq\u7684\u5404\u79cd\u95ee\u9898\uff0c\u4e5f\u6f14\u5316\u51fa\u4e86\u5355\u7ec6\u80de\u6838\u6d4b\u5e8f\u7b49\u65b9\u6cd5\u3002\u603b\u800c\u8a00\u4e4b\uff0cscRNA-seq\u662f\u4e00\u9879\u6781\u5177\u524d\u666f\u7684\u5355\u7ec6\u80de\u6c34\u5e73\u7684\u6d4b\u5e8f\u6280\u672f\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<section style=\"margin-bottom: 0px;padding-right: 1em;padding-left: 1em;font-size: 16px;letter-spacing: 0.578px;text-align: center;white-space: normal;caret-color: rgba(0, 0, 0, 0);display: inline-block;\"><span title=\"\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:9.title1\" style=\"padding: 0.3em 0.5em;display: inline-block;border-radius: 0.5em;font-size: 14px;color: rgb(255, 255, 255);background-color: rgb(71, 193, 168);\"><\/p>\n<p>\u5f80\u671f\u63a8\u8350<br \/><\/p>\n<p><\/span><\/section>\n<p><br \/><\/p>\n<section style=\"margin-top: -1em;margin-bottom: 0px;padding: 20px 10px 10px;font-size: 16px;letter-spacing: 0.578px;text-align: center;white-space: normal;caret-color: rgba(0, 0, 0, 0);border-width: 1px;border-style: solid;border-color: rgb(192, 200, 209);background-color: rgb(239, 239, 239);\">\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<p><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488460&idx=1&sn=3069e8e7f85558703a8ae813375c1552&chksm=cf7c8309f80b0a1fd21e59e711c31141c96f126290f21bf78e2103d5716bdbf41acc36d6ca2a&scene=21#wechat_redirect\" textvalue=\"1\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\"><span style=\"font-size: 14px;\">1<\/span><\/a><span style=\"font-size: 14px;\"> <\/span><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488460&idx=1&sn=3069e8e7f85558703a8ae813375c1552&chksm=cf7c8309f80b0a1fd21e59e711c31141c96f126290f21bf78e2103d5716bdbf41acc36d6ca2a&scene=21#wechat_redirect\" textvalue=\"PCA\u7ed8\u56fe\uff0c\u4ece\u539f\u7406\u5230\u7ed8\u56fe\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\">PCA\u7ed8\u56fe\uff0c\u4ece\u539f\u7406\u5230\u7ed8\u56fe<\/a><br \/><\/p>\n<p><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488459&idx=1&sn=d9a5d7f588994d2602cc1826c2e4f2e5&chksm=cf7c830ef80b0a1883f47601bd39bf81120fc16bc0d02f150a83f40ce52d29fbec17458830f1&scene=21#wechat_redirect\" textvalue=\"\u5c0f\u679c\u7684\u5355\u65e5\u5c0f\u6280\u5de7DAY6\uff1a\u5982\u4f55\u5229\u7528panImmune\u8f7b\u677e\u5206\u6790\u514d\u75ab\u6d78\u6da6\uff1f\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\">2 \u5c0f\u679c\u7684\u5355\u65e5\u5c0f\u6280\u5de7DAY6\uff1a\u5982\u4f55\u5229\u7528panImmune\u8f7b\u677e\u5206\u6790\u514d\u75ab\u6d78\u6da6\uff1f<\/a><br \/><\/p>\n<p><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488458&idx=1&sn=62bd0f5561698111f46d66c681d0eaf8&chksm=cf7c830ff80b0a19e2577c9c70b473076416343b70fbc563d423257619d5abe7104ffa5ea158&scene=21#wechat_redirect\" textvalue=\"3\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\"><span style=\"font-size: 14px;\">3<\/span><\/a><span style=\"font-size: 14px;\"> <\/span><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488458&idx=1&sn=62bd0f5561698111f46d66c681d0eaf8&chksm=cf7c830ff80b0a19e2577c9c70b473076416343b70fbc563d423257619d5abe7104ffa5ea158&scene=21#wechat_redirect\" textvalue=\"Fastp\u8f6f\u4ef6\uff1a\u5904\u7406fastq\u6587\u4ef6\u5b83\u8d85\u68d2\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\">Fastp\u8f6f\u4ef6\uff1a\u5904\u7406fastq\u6587\u4ef6\u5b83\u8d85\u68d2\uff01<\/a><br \/><\/p>\n<p><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488440&idx=1&sn=cf2e3e91652b4c8738ec8b092ede68b6&chksm=cf7c837df80b0a6b09221a3239345182772097dff21e23b36e5ed0f73b08d8c9f2e496c2f3e8&scene=21#wechat_redirect\" textvalue=\"\u9ad8\u5206\u751f\u4fe1SCI\u5e38\u7528\u7684\u80bf\u7624\u514d\u75ab\u5b66\u5355\u7ec6\u80de\u5206\u6790\u5de5\u5177\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\">4 \u9ad8\u5206\u751f\u4fe1SCI\u5e38\u7528\u7684\u80bf\u7624\u514d\u75ab\u5b66\u5355\u7ec6\u80de\u5206\u6790\u5de5\u5177<\/a><br \/><\/p>\n<p><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488459&idx=2&sn=5856888f0c879f1d2989c47da08cb064&chksm=cf7c830ef80b0a1865b8bc9efd54d6726e1b34a3f4b44bf312c19e3f1c0c38eacd6428bb6897&scene=21#wechat_redirect\" 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