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center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><span style=\"text-indent: 2em;\">\u4eca\u5929\u5c0f\u82b1\u4e3a\u5927\u5bb6\u5e26\u6765\u4e00\u6b3e\u975e\u5e38\u65b0\u7684\uff0c\u4f46\u662f\u529f\u80fd\u975e\u5e38\u5f3a\u5927\u7684\u751f\u4fe1\u5206\u6790\u5de5\u5177– MetaPhlAn 4\u3002<\/span><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3351851851851852\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_VMTD7l8Eiav2icPvz6GOuX0iclsl2g.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhF3KjFxEZ5U21MRz3Mdic6rD0pUp64VMTD7l8Eiav2icPvz6GOuX0iclsl2g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">MetaPhlAn\u662f\u4e00\u79cd\u8ba1\u7b97\u5de5\u5177\uff0c\u7528\u4e8e\u4ece\u5143\u57fa\u56e0\u7ec4\u9730\u5f39\u67aa\u6d4b\u5e8f\u6570\u636e\uff08\u5373\u975e16S\uff09\u4e5f\u5c31\u662f\u5b8f\u57fa\u56e0\u7ec4\u6570\u636e\u4e2d\u5206\u6790\u7269\u79cd\u7ea7\u5fae\u751f\u7269\u7fa4\u843d\uff08\u7ec6\u83cc\u3001\u53e4\u7ec6\u83cc\u548c\u771f\u6838\u751f\u7269\uff09\u7684\u7ec4\u6210\u3002\u6700\u65b0\u7248\u7684MetaPhlAn 4\u53d1\u8868\u57282023\u5e742\u670823\u65e5\u300aNature Biotechnology\u300b\u671f\u520a\u4e0a\u3002MetaPhlAn 4\u5229\u7528\u5df2\u77e5\u5fae\u751f\u7269\u57fa\u56e0\u7ec4\u548cMAGs\u7684\u7ec4\u5408\u6269\u5c55\u96c6\uff0c\u5b9a\u4e49\u4e86\u7269\u79cd\u6c34\u5e73\u4e0a\u7684\u57fa\u56e0\u7ec4\u7bb1\uff08SGBs\uff09\uff0c\u4ee5\u8fdb\u884c\u66f4\u5168\u9762\u7684\u5b8f\u57fa\u56e0\u7ec4\u5206\u7c7b\u5b66\u5206\u6790\u3002\u4ece101\u4e07\u4e2a\u539f\u6838\u751f\u7269\u53c2\u8003\u57fa\u56e0\u7ec4\u548c\u5b8f\u57fa\u56e0\u7ec4\u7ec4\u88c5\u57fa\u56e0\u7ec4\u7684\u96c6\u5408\u4e2d\uff0c\u5728\u7269\u79cd\u6c34\u5e73\u4e3a26970\u4e2a\u57fa\u56e0\u7ec4\u7bb1\u5b9a\u4e49\u4e86\u7279\u5f02\u6027\u7684\u6807\u8bb0\u57fa\u56e0\uff0c\u5176\u4e2d4992\u4e2a\u5728\u7269\u79cd\u6c34\u5e73\u4e0a\u5c1a\u672a\u5206\u7c7b\u3002MetaPhlAn 4\u5728\u5168\u7403\u4eba\u7c7b\u80a0\u9053\u5fae\u751f\u7269\u4e2d\u589e\u52a0\u4e86\u7ea620%\u7684\u77ed\u5e8f\u5217\u7269\u79cd\u6ce8\u91ca\uff0c\u800c\u5728\u5c1a\u672a\u5145\u5206\u7814\u7a76\u7684\u73af\u5883\uff08\u5982\u53cd\u520d\u52a8\u7269\u7624\u80c3\u5fae\u751f\u7269\u7ec4\u7b49\uff09\u4e2d\u589e\u52a0\u4e8640%\u3002MetaPhlAn 4\u5728\u7efc\u5408\u8bc4\u4f30\u5206\u6790\u65b9\u9762\u6bd4\u73b0\u6709\u53ef\u66ff\u4ee3\u65b9\u6848\u66f4\u51c6\u786e\uff0c\u540c\u65f6\u8fd8\u53ef\u4ee5\u5bf9\u672a\u57f9\u517b\u5206\u79bb\u7684\u751f\u7269\u4f53\u8fdb\u884c\u51c6\u786e\u5b9a\u91cf\u3002\u603b\u800c\u8a00\u4e4b\uff0cMetaPhlAn 4\u662f\u5b8f\u57fa\u56e0\u7ec4\u7269\u79cd\u6ce8\u91ca\u4e2d\u4e00\u4e2a\u65b9\u4fbf\u800c\u4e14\u51c6\u786e\u7684\u6700\u65b0\u5de5\u5177\u3002<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u63a5\u4e0b\u6765\u5c0f\u82b1\u5c31\u4e3a\u5927\u5bb6\u5e26\u6765MetaPhlAn 4\u7684\u5b89\u88c5\u548c\u4f7f\u7528\u6559\u7a0b\uff0c\u4e0b\u9762\u7684\u64cd\u4f5c\u90fd\u8981\u5728\u670d\u52a1\u5668\u4e0a\u8fdb\u884c\uff0c\u5982\u679c\u6ca1\u6709\u670d\u52a1\u5668\uff0c\u90a3\u5c0f\u82b1\u4e5f\u6ca1\u6cd5\u5566\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e00\u3001\u521b\u5efaconda\u73af\u5883<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">conda create –name mpa -c bioconda python=3.7<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u521b\u5efa\u4e00\u4e2a\u540d\u53ebmpa\u7684\u73af\u5883<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e8c\u3001\u5b89\u88c5metaphlan4<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">source activate mpa<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">conda install -c bioconda metaphlan<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e00\u6b65\u53ef\u80fd\u4f1a\u5b89\u88c5\u5f88\u4e45\uff0c\u6709\u65f6\u5019\u53ef\u80fd\u4f1a\u51fa\u73b0\u5b89\u88c5\u4e0d\u4e0a\u7684\u95ee\u9898\uff0c\u8fd9\u65f6\u5019\u6211\u4eec\u53ef\u4ee5\u7528pip\u6765\u5b89\u88c5\u3002<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e09\u3001\u5b89\u88c5\u6570\u636e\u5e93<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">metaphlan –install<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u662f\u6700\u9ebb\u70e6\u7684\u4e00\u6b65\uff08\u4e00\u822c\u7528\u8fd9\u4e2a\u547d\u4ee4\u90fd\u5b89\u88c5\u4e0d\u4e0a\uff09\uff0c\u4e0b\u8f7d\u901f\u5ea6\u8f83\u6162\u4e14\u603b\u517120\u591aG\uff0c\u5efa\u8bae\u53bb\u5b98\u7f51\u4e0b\u8f7d\uff0c\u4e0b\u8f7d\u6700\u65b0\u7684\u90a3\u4e2a\u6570\u636e\u5e93\u3002<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u516d\u4e2a\u6587\u4ef6\u5efa\u8bae\u90fd\u4e0b\u8f7d\uff0c\u7f51\u5740\u5206\u522b\u662f\uff1a<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><span style=\"color: rgb(16, 116, 231);\">http:\/\/cmprod1.cibio.unitn.it\/biobakery4\/metaphlan_databases\/<\/span><\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><span style=\"color: rgb(16, 116, 231);\">http:\/\/cmprod1.cibio.unitn.it\/biobakery4\/metaphlan_databases\/bowtie2_indexes\/<\/span><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.14612868047982552\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_pVz4H4iaPBib4EaESjVCS8dMSNA3SA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhFSiaicOt1R9rkgvPlBxtmCErc2ButpVz4H4iaPBib4EaESjVCS8dMSNA3SA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"917\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.08602150537634409\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_bd7U9lTh4o3sia15pG7eF79XBcagtQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhFaKia32d2m14Eo9mfCgbM3aTDpNbd7U9lTh4o3sia15pG7eF79XBcagtQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"837\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 2em;text-wrap: wrap;\"><br \/><\/p>\n<section style=\"text-wrap: wrap;text-indent: 0em;\">\u8fd9\u51e0\u4e2a\u6587\u4ef6\u5168\u90e8\u90fd\u8981\u653e\u5728metaphlan_databases\u8fd9\u4e2a\u6587\u4ef6\u5939\u91cc\u9762\uff0c\u8def\u5f84\u4e00\u822c\u662f\u8fd9\u4e2a\uff1a<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">path\/to\/anaconda3\/envs\/mpa\/lib\/python3.7\/site-packages\/metaphlan\/metaphlan_databases<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><span style=\"text-indent: 2em;\">\u6ce8\u610f\u6211\u524d\u9762\u8fd9\u4e2apath\/to\/\u662f\u5927\u5bb6\u653eanaconda3\u7684\u6587\u4ef6\u8def\u5f84\u3002<\/span><\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u540e\u9762\u8fd0\u884cmetaphlan4\u8dd1\u4e00\u4e2a\u6570\u636e\u5c31\u4f1a\u81ea\u52a8\u5efa\u5e93\u3002\u5efa\u5e93\u540e\u7684metaphlan_databases\u5c31\u6709\u8fd9\u4e9b\u6587\u4ef6\uff1a<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.1824074074074074\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_WTtiauzvR3Vp12UOQBLCKWO3xibOyg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhFIdicBQqQ7JtKiazq75qSmZ1KwDMOWTtiauzvR3Vp12UOQBLCKWO3xibOyg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u6ce8\u610fmpa_latest\u8fd9\u4e2a\u6587\u4ef6\uff0c\u5982\u679c\u60a8\u6ca1\u6709\u7684\u8bdd\uff0c\u53ef\u4ee5\u81ea\u5df1vim\u5199\u4e00\u4e2a\uff0c\u91cc\u9762\u7684\u5185\u5bb9\u4e5f\u5c31\u662f\u4e0b\u8f7d\u7684\u6570\u636e\u5e93\u7684\u540d\u5b57\uff1a<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4981481481481482\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_DzdcEwO7AiaBvnL7rDejib0IUWVEXw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhFhQFN38bjXwQibUfflzFEQnDnRsMDzdcEwO7AiaBvnL7rDejib0IUWVEXw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u56db\u3001\u8fd0\u884c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<section style=\"text-wrap: wrap;text-indent: 0em;\">\u53cc\u7aef\u6570\u636e\uff1a<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">metaphlan *.fastq1,*.fastq2 --input_type fastq -o *.txt --nproc <span class=\"code-snippet__number\">10<\/span> --stat_q <span class=\"code-snippet__number\">0.1<\/span> --bowtie2out bowtie2out_*.bz2<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><span style=\"text-indent: 2em;\">\u4e5f\u53ef\u4ee5\u5355\u7aef\uff0c\u5c31\u53ea\u8f93\u5165\u4e00\u4e2afastq\u6570\u636e\uff0c\u6ce8\u610ffastq1\u548cfastq2\u4e4b\u95f4\u6709\u4e2a\u9017\u53f7\u3002<\/span><\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u5176\u4e2d\u53c2\u6570\u7684\u542b\u4e49\uff1a<\/p>\n<\/section>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-width: 1px;border-radius: 8px;border-style: dotted;border-color: rgb(0, 0, 0);overflow: hidden;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin: 15px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);padding-right: 15px;padding-left: 15px;\">\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">–input_type\u8f93\u5165\u6570\u636e\u7c7b\u578b<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">–stat_q\u8d8a\u4f4e\u5047\u9633\u6027\u7387\u8d8a\u9ad8\uff0c\u9ed8\u8ba4\u662f0.2\uff0c\u4f46\u662f\u53ef\u4ee5\u6309\u7167\u6570\u636e\u5177\u4f53\u60c5\u51b5\u8c03\u6574\uff0c0.1\u5e94\u8be5\u662f\u53ef\u4ee5\u63a5\u53d7\u7684<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">–nproc\u7ebf\u7a0b<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e94\u3001\u6570\u636e\u5408\u5e76\u622a\u53d6<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<section style=\"text-wrap: wrap;text-indent: 0em;\">\u591a\u4e2a\u6570\u636e\u8fd0\u884c\u5b8c\u6210\u540e\uff0c\u9700\u8981\u5bf9\u591a\u4e2a\u7ed3\u679c\u8fdb\u884c\u5408\u5e76\uff08\u4e0d\u540c\u7248\u672c\u7684\u6570\u636e\u4e0d\u80fd\u5408\u5e76\uff09 \uff1a<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">merge_metaphlan_tables.py metaphlan_output*.txt > output\/merged_abundance_table.txt<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7b5b\u9009\u51fa\u4e0d\u540c\u6c34\u5e73\u7269\u79cd\u4e30\u5ea6\u8868\u683c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">grep -E <span class=\"code-snippet__string\">'(s__)|(clade_name)'<\/span> merged_abundance_table.txt |grep -v <span class=\"code-snippet__string\">'t__'<\/span>|sed <span class=\"code-snippet__string\">'s\/^.*s__\/\/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/ \/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/t\/g'<\/span> > merged_abundance_table_species.txt<\/span><\/code><code><span class=\"code-snippet_outer\">grep -E <span class=\"code-snippet__string\">'(g__)|(clade_name)'<\/span> merged_abundance_table.txt |grep -v <span class=\"code-snippet__string\">'s__'<\/span>|sed <span class=\"code-snippet__string\">'s\/^.*g__\/\/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/ \/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/t\/g'<\/span> > merged_abundance_table_genus.txt<\/span><\/code><code><span class=\"code-snippet_outer\">grep -E <span class=\"code-snippet__string\">'(f__)|(clade_name)'<\/span> merged_abundance_table.txt |grep -v <span class=\"code-snippet__string\">'g__'<\/span>|sed <span class=\"code-snippet__string\">'s\/^.*f__\/\/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/ \/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/t\/g'<\/span> > merged_abundance_table_family.txt<\/span><\/code><code><span class=\"code-snippet_outer\">grep -E <span class=\"code-snippet__string\">'(o__)|(clade_name)'<\/span> merged_abundance_table.txt |grep -v <span class=\"code-snippet__string\">'f__'<\/span>|sed <span class=\"code-snippet__string\">'s\/^.*o__\/\/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/ \/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/t\/g'<\/span> > merged_abundance_table_order.txt<\/span><\/code><code><span class=\"code-snippet_outer\">grep -E <span class=\"code-snippet__string\">'(c__)|(clade_name)'<\/span> merged_abundance_table.txt |grep -v <span class=\"code-snippet__string\">'o__'<\/span>|sed <span class=\"code-snippet__string\">'s\/^.*c__\/\/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/ \/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/t\/g'<\/span> > merged_abundance_table_class.txt<\/span><\/code><code><span class=\"code-snippet_outer\">grep -E <span class=\"code-snippet__string\">'(p__)|(clade_name)'<\/span> merged_abundance_table.txt |grep -v <span class=\"code-snippet__string\">'c__'<\/span>|sed <span class=\"code-snippet__string\">'s\/^.*p__\/\/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/ \/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/t\/g'<\/span> > merged_abundance_table_phylum.txt<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u516d\u3001\u811a\u672c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u5927\u5bb6\u53ef\u4ee5\u4e00\u4e2a\u4e00\u4e2a\u6570\u636e\u7684\u8dd1\uff0c\u5f53\u7136\u4e5f\u53ef\u4ee5\u5199\u4e2a\u5faa\u73af\uff0c\u8fd9\u6837\u6548\u7387\u66f4\u9ad8\uff0c\u8fd9\u4e2a\u662f\u5c0f\u82b1\u81ea\u5df1\u5199\u7684\u4e00\u4e2a\u6700\u7b80\u5355\u7684shell\u811a\u672c\uff1a<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span> i <span class=\"code-snippet__keyword\">in<\/span> `ls data\/split`; do<\/span><\/code><code><span class=\"code-snippet_outer\">metaphlan data\/${i}_1.fq.gz,data\/${i}_2.fq.gz --input_type fastq --nproc <span class=\"code-snippet__number\">30<\/span> \u2013output metaphlan\/${i}.txt --bowtie2out metaphlan-<span class=\"code-snippet__number\">1<\/span>\/${i}.bowtie --stat_q <span class=\"code-snippet__number\">0.05<\/span>; done<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">###split\u6587\u4ef6\u5939\u91cc\u9762\u662f\u6240\u6709\u6570\u636e\u540d\u79f0\u7684\u6587\u4ef6<\/span><\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u8fd0\u884c\u5b8c\u7684\u7ed3\u679c\u5927\u6982\u662f\u8fd9\u6837\uff1a<br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4981481481481482\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_Ig7mbXHeSSVurLb6mhvY6VpibwOCcg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhF2heGjW9F7QNgg5ByTkECNLO1iaIg7mbXHeSSVurLb6mhvY6VpibwOCcg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<p>\u8fd9\u5c31\u662f\u672c\u6b21\u6559\u7a0b\u7684\u5168\u90e8\u5185\u5bb9\u5566\uff0c\u540e\u7eed\u5c0f\u82b1\u4f1a\u4e3a\u5927\u5bb6\u5e26\u6765\u66f4\u591a\u7684\u5b8f\u57fa\u56e0\u7ec4\u548c\u6269\u589e\u5b50\u8f6f\u4ef6\u7684\u4f7f\u7528\u6559\u7a0b\u3002<\/p>\n<p><br \/><\/p>\n<p>\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1 – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/p>\n<p><br \/><\/p>\n<p>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a18502195490\uff08\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/p>\n<\/section>\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row;transform: translate3d(40px, 0px, 0px);margin-bottom: -40px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 26px;height: 26px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);border-left: 1px solid rgb(160, 160, 160);border-bottom-left-radius: 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_pBN4r4MaBCk9p9v16yZwvMvS5nM7sQ-3.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"895\" style=\"\" data-recalc-dims=\"1\" \/><\/span><\/a><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3488888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_uKaqczIP6EMQhkT9sW7c1bKv6z9AAg-3.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdD9CpdQZl2iaySUTmNepgg2BpMuKaqczIP6EMQhkT9sW7c1bKv6z9AAg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p powered-by=\"xiumi.us\">\uff08\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8df3\u8f6c\uff09<\/p>\n<section style=\"text-align: left;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;min-width: 10%;vertical-align: top;padding-right: 20px;\">\n<section style=\"transform: translate3d(20px, 0px, 0px);margin: 10px 0% -30px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);text-align: justify;font-size: 12px;\">\n<p><strong><img decoding=\"async\" loading=\"lazy\" data-ratio=\"0.866\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_QLicgvJO8sDf3tA5Zxgk2Fx6bh9wHQ.gif?resize=18%2C15\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_gif\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhFdxGAxNkXwyUDdXQZ2RMYC9iacDQLicgvJO8sDf3tA5Zxgk2Fx6bh9wHQ\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"500\" height=\"15\" style=\"width: 18px;height: 15px;\" width=\"18\" data-recalc-dims=\"1\" \/> \u70b9\u4e00\u4e0b\u9605\u8bfb\u539f\u6587\u4e86\u89e3\u66f4\u591a\u8d44\u8baf<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 50px;height: 40px;vertical-align: top;overflow: hidden;\">\n<section style=\"text-align: center;margin-right: 0%;margin-left: 0%;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.7709090909090909\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_q83M51aXmEgdfHKxFwFNlFETpxSvBg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhFwztiaHRoCmRMBiaojWOlhVdWmEq83M51aXmEgdfHKxFwFNlFETpxSvBg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"275\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>MetaPhlAn 4\u2014\u5b8f\u57fa\u56e0\u7ec4\u7269\u79cd\u6ce8\u91ca\u5de5\u5177 \u5c0f\u82b1 \u751f\u4fe1\u679c 202 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/56481"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=56481"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/56481\/revisions"}],"predecessor-version":[{"id":56492,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/56481\/revisions\/56492"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=56481"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=56481"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=56481"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

{"id":56481,"date":"2024-06-16T17:57:51","date_gmt":"2024-06-16T09:57:51","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=56481"},"modified":"2024-06-16T17:57:51","modified_gmt":"2024-06-16T09:57:51","slug":"metaphlan-4-%e5%ae%8f%e5%9f%ba%e5%9b%a0%e7%bb%84%e7%89%a9%e7%a7%8d%e6%b3%a8%e9%87%8a%e5%b7%a5%e5%85%b7-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=56481","title":{"rendered":"MetaPhlAn 4\u2014\u5b8f\u57fa\u56e0\u7ec4\u7269\u79cd\u6ce8\u91ca\u5de5\u5177"},"content":{"rendered":"

\n
\n<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner,body{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media{padding:20px;overflow:hidden;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}@media screen and (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media_content{overflow:hidden;color:#333;font-size:17px;word-wrap:break-word;-webkit-hyphens:auto;-ms-hyphens:auto;hyphens:auto;text-align:justify;position:relative;z-index:0}.rich_media_content *{max-width:100%!important;box-sizing:border-box!important;-webkit-box-sizing:border-box!important;word-wrap:break-word!important}.rich_media_content p{clear:both;min-height:1em}.rich_media_content em{font-style:italic}.rich_media_content fieldset{min-width:0}.rich_media_content .list-paddingleft-1,.rich_media_content .list-paddingleft-2,.rich_media_content .list-paddingleft-3{padding-left:2.2em}.rich_media_content .list-paddingleft-1 .list-paddingleft-2,.rich_media_content .list-paddingleft-2 .list-paddingleft-2,.rich_media_content .list-paddingleft-3 .list-paddingleft-2{padding-left:30px}.rich_media_content .list-paddingleft-1{padding-left:1.2em}.rich_media_content .list-paddingleft-3{padding-left:3.2em}.rich_media_content .code-snippet,.rich_media_content .code-snippet__fix{max-width:1000%!important}.rich_media_content .code-snippet *,.rich_media_content .code-snippet__fix *{max-width:1000%!important}.rich_media_title{font-size:22px;line-height:1.4;margin-bottom:13px;padding-bottom:13px;border-bottom:1px solid 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data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"MetaPhlAn 4\u2014\u5b8f\u57fa\u56e0\u7ec4\u7269\u79cd\u6ce8\u91ca\u5de5\u5177\",\"time\":\"2023-08-17 19:01:12\",\"timeStamp\":\"1692270072\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491501&idx=5&sn=041de0defb1c6f4830d8618d92399157&chksm=cf7c8f68f80b067e97121c08e404658dca9c5a685b3721e00d8a8fd5fe7b75161e825a38d900#rd\" target=\"_blank\" rel=\"noopener\">MetaPhlAn 4\u2014\u5b8f\u57fa\u56e0\u7ec4\u7269\u79cd\u6ce8\u91ca\u5de5\u5177<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u82b1 <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-08-17 19:01:12<\/em><\/div>\n<p><content><\/p>\n<div class=\"reprint\">\n<p>\u8f6c\u81ea\u516c\u4f17\u53f7\uff1a\u751f\u4fe1\u82b1<br \/><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484134&idx=1&sn=3f485669db1b3620f08adc3a2f742be2\" target=\"_blank\" title=\"\u9605\u8bfb\u539f\u6587\" rel=\"noopener\">http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484134&idx=1&sn=3f485669db1b3620f08adc3a2f742be2<\/a><\/p>\n<\/div>\n<section style=\"font-size: 14px;margin-bottom: 24px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(65, 128, 216);\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;padding-left: 12px;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);\" powered-by=\"xiumi.us\">\n<p><strong>\u70b9\u51fb\u84dd\u5b57 \u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(201, 229, 70);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><span style=\"text-indent: 2em;\">\u4eca\u5929\u5c0f\u82b1\u4e3a\u5927\u5bb6\u5e26\u6765\u4e00\u6b3e\u975e\u5e38\u65b0\u7684\uff0c\u4f46\u662f\u529f\u80fd\u975e\u5e38\u5f3a\u5927\u7684\u751f\u4fe1\u5206\u6790\u5de5\u5177– MetaPhlAn 4\u3002<\/span><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3351851851851852\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_VMTD7l8Eiav2icPvz6GOuX0iclsl2g.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhF3KjFxEZ5U21MRz3Mdic6rD0pUp64VMTD7l8Eiav2icPvz6GOuX0iclsl2g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">MetaPhlAn\u662f\u4e00\u79cd\u8ba1\u7b97\u5de5\u5177\uff0c\u7528\u4e8e\u4ece\u5143\u57fa\u56e0\u7ec4\u9730\u5f39\u67aa\u6d4b\u5e8f\u6570\u636e\uff08\u5373\u975e16S\uff09\u4e5f\u5c31\u662f\u5b8f\u57fa\u56e0\u7ec4\u6570\u636e\u4e2d\u5206\u6790\u7269\u79cd\u7ea7\u5fae\u751f\u7269\u7fa4\u843d\uff08\u7ec6\u83cc\u3001\u53e4\u7ec6\u83cc\u548c\u771f\u6838\u751f\u7269\uff09\u7684\u7ec4\u6210\u3002\u6700\u65b0\u7248\u7684MetaPhlAn 4\u53d1\u8868\u57282023\u5e742\u670823\u65e5\u300aNature Biotechnology\u300b\u671f\u520a\u4e0a\u3002MetaPhlAn 4\u5229\u7528\u5df2\u77e5\u5fae\u751f\u7269\u57fa\u56e0\u7ec4\u548cMAGs\u7684\u7ec4\u5408\u6269\u5c55\u96c6\uff0c\u5b9a\u4e49\u4e86\u7269\u79cd\u6c34\u5e73\u4e0a\u7684\u57fa\u56e0\u7ec4\u7bb1\uff08SGBs\uff09\uff0c\u4ee5\u8fdb\u884c\u66f4\u5168\u9762\u7684\u5b8f\u57fa\u56e0\u7ec4\u5206\u7c7b\u5b66\u5206\u6790\u3002\u4ece101\u4e07\u4e2a\u539f\u6838\u751f\u7269\u53c2\u8003\u57fa\u56e0\u7ec4\u548c\u5b8f\u57fa\u56e0\u7ec4\u7ec4\u88c5\u57fa\u56e0\u7ec4\u7684\u96c6\u5408\u4e2d\uff0c\u5728\u7269\u79cd\u6c34\u5e73\u4e3a26970\u4e2a\u57fa\u56e0\u7ec4\u7bb1\u5b9a\u4e49\u4e86\u7279\u5f02\u6027\u7684\u6807\u8bb0\u57fa\u56e0\uff0c\u5176\u4e2d4992\u4e2a\u5728\u7269\u79cd\u6c34\u5e73\u4e0a\u5c1a\u672a\u5206\u7c7b\u3002MetaPhlAn 4\u5728\u5168\u7403\u4eba\u7c7b\u80a0\u9053\u5fae\u751f\u7269\u4e2d\u589e\u52a0\u4e86\u7ea620%\u7684\u77ed\u5e8f\u5217\u7269\u79cd\u6ce8\u91ca\uff0c\u800c\u5728\u5c1a\u672a\u5145\u5206\u7814\u7a76\u7684\u73af\u5883\uff08\u5982\u53cd\u520d\u52a8\u7269\u7624\u80c3\u5fae\u751f\u7269\u7ec4\u7b49\uff09\u4e2d\u589e\u52a0\u4e8640%\u3002MetaPhlAn 4\u5728\u7efc\u5408\u8bc4\u4f30\u5206\u6790\u65b9\u9762\u6bd4\u73b0\u6709\u53ef\u66ff\u4ee3\u65b9\u6848\u66f4\u51c6\u786e\uff0c\u540c\u65f6\u8fd8\u53ef\u4ee5\u5bf9\u672a\u57f9\u517b\u5206\u79bb\u7684\u751f\u7269\u4f53\u8fdb\u884c\u51c6\u786e\u5b9a\u91cf\u3002\u603b\u800c\u8a00\u4e4b\uff0cMetaPhlAn 4\u662f\u5b8f\u57fa\u56e0\u7ec4\u7269\u79cd\u6ce8\u91ca\u4e2d\u4e00\u4e2a\u65b9\u4fbf\u800c\u4e14\u51c6\u786e\u7684\u6700\u65b0\u5de5\u5177\u3002<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u63a5\u4e0b\u6765\u5c0f\u82b1\u5c31\u4e3a\u5927\u5bb6\u5e26\u6765MetaPhlAn 4\u7684\u5b89\u88c5\u548c\u4f7f\u7528\u6559\u7a0b\uff0c\u4e0b\u9762\u7684\u64cd\u4f5c\u90fd\u8981\u5728\u670d\u52a1\u5668\u4e0a\u8fdb\u884c\uff0c\u5982\u679c\u6ca1\u6709\u670d\u52a1\u5668\uff0c\u90a3\u5c0f\u82b1\u4e5f\u6ca1\u6cd5\u5566\u3002<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e00\u3001\u521b\u5efaconda\u73af\u5883<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">conda create –name mpa -c bioconda python=3.7<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u521b\u5efa\u4e00\u4e2a\u540d\u53ebmpa\u7684\u73af\u5883<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e8c\u3001\u5b89\u88c5metaphlan4<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">source activate mpa<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">conda install -c bioconda metaphlan<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u4e00\u6b65\u53ef\u80fd\u4f1a\u5b89\u88c5\u5f88\u4e45\uff0c\u6709\u65f6\u5019\u53ef\u80fd\u4f1a\u51fa\u73b0\u5b89\u88c5\u4e0d\u4e0a\u7684\u95ee\u9898\uff0c\u8fd9\u65f6\u5019\u6211\u4eec\u53ef\u4ee5\u7528pip\u6765\u5b89\u88c5\u3002<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e09\u3001\u5b89\u88c5\u6570\u636e\u5e93<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">metaphlan –install<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u662f\u6700\u9ebb\u70e6\u7684\u4e00\u6b65\uff08\u4e00\u822c\u7528\u8fd9\u4e2a\u547d\u4ee4\u90fd\u5b89\u88c5\u4e0d\u4e0a\uff09\uff0c\u4e0b\u8f7d\u901f\u5ea6\u8f83\u6162\u4e14\u603b\u517120\u591aG\uff0c\u5efa\u8bae\u53bb\u5b98\u7f51\u4e0b\u8f7d\uff0c\u4e0b\u8f7d\u6700\u65b0\u7684\u90a3\u4e2a\u6570\u636e\u5e93\u3002<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u8fd9\u516d\u4e2a\u6587\u4ef6\u5efa\u8bae\u90fd\u4e0b\u8f7d\uff0c\u7f51\u5740\u5206\u522b\u662f\uff1a<\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><span style=\"color: rgb(16, 116, 231);\">http:\/\/cmprod1.cibio.unitn.it\/biobakery4\/metaphlan_databases\/<\/span><\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><span style=\"color: rgb(16, 116, 231);\">http:\/\/cmprod1.cibio.unitn.it\/biobakery4\/metaphlan_databases\/bowtie2_indexes\/<\/span><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.14612868047982552\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_pVz4H4iaPBib4EaESjVCS8dMSNA3SA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhFSiaicOt1R9rkgvPlBxtmCErc2ButpVz4H4iaPBib4EaESjVCS8dMSNA3SA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"917\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.08602150537634409\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_bd7U9lTh4o3sia15pG7eF79XBcagtQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhFaKia32d2m14Eo9mfCgbM3aTDpNbd7U9lTh4o3sia15pG7eF79XBcagtQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"837\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 2em;text-wrap: wrap;\"><br \/><\/p>\n<section style=\"text-wrap: wrap;text-indent: 0em;\">\u8fd9\u51e0\u4e2a\u6587\u4ef6\u5168\u90e8\u90fd\u8981\u653e\u5728metaphlan_databases\u8fd9\u4e2a\u6587\u4ef6\u5939\u91cc\u9762\uff0c\u8def\u5f84\u4e00\u822c\u662f\u8fd9\u4e2a\uff1a<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">path\/to\/anaconda3\/envs\/mpa\/lib\/python3.7\/site-packages\/metaphlan\/metaphlan_databases<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><span style=\"text-indent: 2em;\">\u6ce8\u610f\u6211\u524d\u9762\u8fd9\u4e2apath\/to\/\u662f\u5927\u5bb6\u653eanaconda3\u7684\u6587\u4ef6\u8def\u5f84\u3002<\/span><\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u540e\u9762\u8fd0\u884cmetaphlan4\u8dd1\u4e00\u4e2a\u6570\u636e\u5c31\u4f1a\u81ea\u52a8\u5efa\u5e93\u3002\u5efa\u5e93\u540e\u7684metaphlan_databases\u5c31\u6709\u8fd9\u4e9b\u6587\u4ef6\uff1a<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.1824074074074074\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_WTtiauzvR3Vp12UOQBLCKWO3xibOyg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhFIdicBQqQ7JtKiazq75qSmZ1KwDMOWTtiauzvR3Vp12UOQBLCKWO3xibOyg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u6ce8\u610fmpa_latest\u8fd9\u4e2a\u6587\u4ef6\uff0c\u5982\u679c\u60a8\u6ca1\u6709\u7684\u8bdd\uff0c\u53ef\u4ee5\u81ea\u5df1vim\u5199\u4e00\u4e2a\uff0c\u91cc\u9762\u7684\u5185\u5bb9\u4e5f\u5c31\u662f\u4e0b\u8f7d\u7684\u6570\u636e\u5e93\u7684\u540d\u5b57\uff1a<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4981481481481482\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_DzdcEwO7AiaBvnL7rDejib0IUWVEXw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhFhQFN38bjXwQibUfflzFEQnDnRsMDzdcEwO7AiaBvnL7rDejib0IUWVEXw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u56db\u3001\u8fd0\u884c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<section style=\"text-wrap: wrap;text-indent: 0em;\">\u53cc\u7aef\u6570\u636e\uff1a<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">metaphlan *.fastq1,*.fastq2 --input_type fastq -o *.txt --nproc <span class=\"code-snippet__number\">10<\/span> --stat_q <span class=\"code-snippet__number\">0.1<\/span> --bowtie2out bowtie2out_*.bz2<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\"><span style=\"text-indent: 2em;\">\u4e5f\u53ef\u4ee5\u5355\u7aef\uff0c\u5c31\u53ea\u8f93\u5165\u4e00\u4e2afastq\u6570\u636e\uff0c\u6ce8\u610ffastq1\u548cfastq2\u4e4b\u95f4\u6709\u4e2a\u9017\u53f7\u3002<\/span><\/p>\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u5176\u4e2d\u53c2\u6570\u7684\u542b\u4e49\uff1a<\/p>\n<\/section>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-width: 1px;border-radius: 8px;border-style: dotted;border-color: rgb(0, 0, 0);overflow: hidden;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin: 15px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);padding-right: 15px;padding-left: 15px;\">\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">–input_type\u8f93\u5165\u6570\u636e\u7c7b\u578b<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">–stat_q\u8d8a\u4f4e\u5047\u9633\u6027\u7387\u8d8a\u9ad8\uff0c\u9ed8\u8ba4\u662f0.2\uff0c\u4f46\u662f\u53ef\u4ee5\u6309\u7167\u6570\u636e\u5177\u4f53\u60c5\u51b5\u8c03\u6574\uff0c0.1\u5e94\u8be5\u662f\u53ef\u4ee5\u63a5\u53d7\u7684<\/span><\/p>\n<p><span style=\"text-shadow: rgb(255, 253, 231) 0.766044px 0.642788px 2px;\">–nproc\u7ebf\u7a0b<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e94\u3001\u6570\u636e\u5408\u5e76\u622a\u53d6<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<section style=\"text-wrap: wrap;text-indent: 0em;\">\u591a\u4e2a\u6570\u636e\u8fd0\u884c\u5b8c\u6210\u540e\uff0c\u9700\u8981\u5bf9\u591a\u4e2a\u7ed3\u679c\u8fdb\u884c\u5408\u5e76\uff08\u4e0d\u540c\u7248\u672c\u7684\u6570\u636e\u4e0d\u80fd\u5408\u5e76\uff09 \uff1a<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">merge_metaphlan_tables.py metaphlan_output*.txt > output\/merged_abundance_table.txt<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7b5b\u9009\u51fa\u4e0d\u540c\u6c34\u5e73\u7269\u79cd\u4e30\u5ea6\u8868\u683c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">grep -E <span class=\"code-snippet__string\">'(s__)|(clade_name)'<\/span> merged_abundance_table.txt |grep -v <span class=\"code-snippet__string\">'t__'<\/span>|sed <span class=\"code-snippet__string\">'s\/^.*s__\/\/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/ \/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/t\/g'<\/span> > merged_abundance_table_species.txt<\/span><\/code><code><span class=\"code-snippet_outer\">grep -E <span class=\"code-snippet__string\">'(g__)|(clade_name)'<\/span> merged_abundance_table.txt |grep -v <span class=\"code-snippet__string\">'s__'<\/span>|sed <span class=\"code-snippet__string\">'s\/^.*g__\/\/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/ \/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/t\/g'<\/span> > merged_abundance_table_genus.txt<\/span><\/code><code><span class=\"code-snippet_outer\">grep -E <span class=\"code-snippet__string\">'(f__)|(clade_name)'<\/span> merged_abundance_table.txt |grep -v <span class=\"code-snippet__string\">'g__'<\/span>|sed <span class=\"code-snippet__string\">'s\/^.*f__\/\/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/ \/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/t\/g'<\/span> > merged_abundance_table_family.txt<\/span><\/code><code><span class=\"code-snippet_outer\">grep -E <span class=\"code-snippet__string\">'(o__)|(clade_name)'<\/span> merged_abundance_table.txt |grep -v <span class=\"code-snippet__string\">'f__'<\/span>|sed <span class=\"code-snippet__string\">'s\/^.*o__\/\/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/ \/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/t\/g'<\/span> > merged_abundance_table_order.txt<\/span><\/code><code><span class=\"code-snippet_outer\">grep -E <span class=\"code-snippet__string\">'(c__)|(clade_name)'<\/span> merged_abundance_table.txt |grep -v <span class=\"code-snippet__string\">'o__'<\/span>|sed <span class=\"code-snippet__string\">'s\/^.*c__\/\/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/ \/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/t\/g'<\/span> > merged_abundance_table_class.txt<\/span><\/code><code><span class=\"code-snippet_outer\">grep -E <span class=\"code-snippet__string\">'(p__)|(clade_name)'<\/span> merged_abundance_table.txt |grep -v <span class=\"code-snippet__string\">'c__'<\/span>|sed <span class=\"code-snippet__string\">'s\/^.*p__\/\/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/ \/g'<\/span>|sed <span class=\"code-snippet__string\">'s\/ \/t\/g'<\/span> > merged_abundance_table_phylum.txt<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>\u516d\u3001\u811a\u672c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;text-wrap: wrap;\">\u5927\u5bb6\u53ef\u4ee5\u4e00\u4e2a\u4e00\u4e2a\u6570\u636e\u7684\u8dd1\uff0c\u5f53\u7136\u4e5f\u53ef\u4ee5\u5199\u4e2a\u5faa\u73af\uff0c\u8fd9\u6837\u6548\u7387\u66f4\u9ad8\uff0c\u8fd9\u4e2a\u662f\u5c0f\u82b1\u81ea\u5df1\u5199\u7684\u4e00\u4e2a\u6700\u7b80\u5355\u7684shell\u811a\u672c\uff1a<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span> i <span class=\"code-snippet__keyword\">in<\/span> `ls data\/split`; do<\/span><\/code><code><span class=\"code-snippet_outer\">metaphlan data\/${i}_1.fq.gz,data\/${i}_2.fq.gz --input_type fastq --nproc <span class=\"code-snippet__number\">30<\/span> \u2013output metaphlan\/${i}.txt --bowtie2out metaphlan-<span class=\"code-snippet__number\">1<\/span>\/${i}.bowtie --stat_q <span class=\"code-snippet__number\">0.05<\/span>; done<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">###split\u6587\u4ef6\u5939\u91cc\u9762\u662f\u6240\u6709\u6570\u636e\u540d\u79f0\u7684\u6587\u4ef6<\/span><\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u8fd0\u884c\u5b8c\u7684\u7ed3\u679c\u5927\u6982\u662f\u8fd9\u6837\uff1a<br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4981481481481482\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_Ig7mbXHeSSVurLb6mhvY6VpibwOCcg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSaLGQ9HZpLCBZjJIfkRkkhF2heGjW9F7QNgg5ByTkECNLO1iaIg7mbXHeSSVurLb6mhvY6VpibwOCcg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section 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