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\/><\/p>\n<p>hello\uff0c\u4f60\u77e5\u9053\u5982\u4f55\u4f7f\u7528SingleCellExperiment\u5305\u6765\u5206\u6790\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u6570\u636e\u561b\uff1f\u4eca\u5929\u5927\u6d77\u54e5\u5c31\u6765\u6559\u5927\u5bb6\u5982\u4f55\u5177\u4f53\u4f7f\u7528\u8fd9\u4e2aR\u5305\uff0c\u5e72\u8d27\u6ee1\u6ee1\uff0c\u611f\u5174\u8da3\u7684\u8bdd\u5c31\u548c\u5927\u6d77\u54e5\u4e00\u8d77\u770b\u4e0b\u53bb\u5427\uff01<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u8d70\u8fdbSingleCellExperiment<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u9996\u5148\uff0c\u5927\u6d77\u54e5\u6765\u7b80\u5355\u7684\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e0b\u8fd9\u4e2aR\u5305\uff1a<\/p>\n<p>SingleCellExperiment\u5305\u662f\u7528\u4e8e\u5b58\u50a8\u548c\u5206\u6790\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u6570\u636e\u7684R\u8bed\u8a00\u5de5\u5177\u5305\u3002\u5b83\u63d0\u4f9b\u4e86\u4e00\u4e2a\u7075\u6d3b\u7684\u6570\u636e\u7ed3\u6784\uff0c\u53ef\u4ee5\u5bb9\u7eb3\u5355\u7ec6\u80de\u6570\u636e\u4e2d\u7684\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u3001\u5143\u6570\u636e\u548c\u5176\u4ed6\u76f8\u5173\u4fe1\u606f\uff0c\u5e76\u4e3a\u7528\u6237\u63d0\u4f9b\u4e86\u591a\u79cd\u529f\u80fd\u6765\u5904\u7406\u548c\u5206\u6790\u8fd9\u4e9b\u6570\u636e\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>SingleCellExperiment\u5305\u7684\u4e3b\u8981\u7279\u70b9\u5305\u62ec\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"color: rgb(59, 149, 115);\">\u6570\u636e\u5b58\u50a8<\/span><\/strong>\uff1aSingleCellExperiment\u5305\u4f7f\u7528\u4e00\u79cd\u79f0\u4e3aSingleCellExperiment\u7c7b\u7684\u6570\u636e\u7ed3\u6784\u6765\u5b58\u50a8\u5355\u7ec6\u80de\u6570\u636e\u3002\u8be5\u7c7b\u7ee7\u627f\u81eaSummarizedExperiment\u7c7b\uff0c\u56e0\u6b64\u4e5f\u53ef\u4ee5\u4f7f\u7528SummarizedExperiment\u7c7b\u7684\u65b9\u6cd5\u548c\u51fd\u6570\u3002\u8fd9\u4e2a\u6570\u636e\u7ed3\u6784\u65e8\u5728\u7b80\u5316\u5355\u7ec6\u80de\u6570\u636e\u7684\u7ba1\u7406\u548c\u8bbf\u95ee\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"color: rgb(59, 149, 115);\">\u7075\u6d3b\u6027<\/span><\/strong>\uff1aSingleCellExperiment\u5305\u63d0\u4f9b\u4e86\u7075\u6d3b\u7684\u6570\u636e\u5b58\u50a8\u65b9\u5f0f\u3002\u9664\u4e86\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u5916\uff0c\u5b83\u8fd8\u53ef\u4ee5\u5b58\u50a8\u548c\u7ba1\u7406\u964d\u7ef4\u6570\u636e\u3001\u5217\u6570\u636e\u3001\u884c\u6570\u636e\u548c\u5143\u6570\u636e\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"color: rgb(59, 149, 115);\">\u6570\u636e\u5206\u6790<\/span><\/strong>\uff1aSingleCellExperiment\u5305\u63d0\u4f9b\u4e86\u8bb8\u591a\u7528\u4e8e\u5904\u7406\u548c\u5206\u6790\u5355\u7ec6\u80de\u6570\u636e\u7684\u51fd\u6570\u548c\u65b9\u6cd5\u3002\u5b83\u652f\u6301\u5e38\u89c1\u7684\u6570\u636e\u64cd\u4f5c\uff0c\u5982\u5b50\u96c6\u9009\u62e9\u3001\u8fc7\u6ee4\u3001\u6807\u51c6\u5316\u548c\u53d8\u6362\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"color: rgb(59, 149, 115);\">\u53ef\u6269\u5c55\u6027<\/span><\/strong>\uff1aSingleCellExperiment\u5305\u7684\u6570\u636e\u7ed3\u6784\u53ef\u4ee5\u65b9\u4fbf\u5730\u4e0e\u5176\u4ed6\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u5de5\u5177\u548c\u8f6f\u4ef6\u5305\u8fdb\u884c\u4ea4\u4e92\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u603b\u4e4b\uff0cSingleCellExperiment\u5305\u662f\u4e00\u4e2a\u529f\u80fd\u5f3a\u5927\u4e14\u6613\u4e8e\u4f7f\u7528\u7684\u5de5\u5177\u5305\uff0c\u4e13\u95e8\u8bbe\u8ba1\u7528\u4e8e\u5904\u7406\u548c\u5206\u6790\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u6570\u636e\u3002\u5b83\u63d0\u4f9b\u4e86\u7075\u6d3b\u7684\u6570\u636e\u5b58\u50a8\u65b9\u5f0f\u548c\u4e30\u5bcc\u7684\u5206\u6790\u529f\u80fd\uff0c\u4f7f\u7528\u6237\u80fd\u591f\u66f4\u597d\u5730\u7406\u89e3\u548c\u89e3\u91ca\u5355\u7ec6\u80de\u6570\u636e\u3002<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>SingleCellExperiment\u8fdb\u884c\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p><strong>\u5b89\u88c5\u548c\u52a0\u8f7d SingleCellExperiment \u5305<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u9996\u5148\uff0c\u6211\u4eec\u9700\u8981\u786e\u4fdd\u5df2\u5b89\u88c5 SingleCellExperiment \u5305\u3002\u5982\u679c\u5c1a\u672a\u5b89\u88c5\uff0c\u8bf7\u5927\u5bb6\u8fd0\u884c\u4ee5\u4e0b\u4ee3\u7801\u54e6\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span> (!requireNamespace(<span class=\"code-snippet__string\">\"BiocManager\"<\/span>, quietly = <span class=\"code-snippet__literal\">TRUE<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(<span class=\"code-snippet__string\">\"BiocManager\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">BiocManager::install(<span class=\"code-snippet__string\">\"SingleCellExperiment\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u7136\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u52a0\u8f7d SingleCellExperiment \u5305\u4ee5\u5f00\u59cb\u4f7f\u7528\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">library(SingleCellExperiment)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p><strong>\u521b\u5efa SingleCellExperiment \u5bf9\u8c61<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"font-size: 15px;color: rgb(255, 255, 254);line-height: 2;letter-spacing: 2px;padding-right: 15px;padding-left: 15px;font-family: PingFangSC-light;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>1. \u521b\u5efa\u8868\u8fbe\u77e9\u9635<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u9996\u5148\uff0c\u6211\u4eec\u53ef\u4ee5\u521b\u5efa\u4e00\u4e2a\u53ea\u5305\u542b\u8868\u8fbe\u77e9\u9635\u7684 SingleCellExperiment \u5bf9\u8c61\u3002\u5047\u8bbe\u6211\u4eec\u6709\u4e00\u4e2a 10×10 \u7684\u8868\u8fbe\u77e9\u9635\uff0c\u53ef\u4ee5\u4f7f\u7528\u4ee5\u4e0b\u4ee3\u7801\u521b\u5efa\u4e00\u4e2a SingleCellExperiment \u5bf9\u8c61\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">counts <- matrix(rpois(<span class=\"code-snippet__number\">100<\/span>, lambda = <span class=\"code-snippet__number\">10<\/span>), ncol=<span class=\"code-snippet__number\">10<\/span>, nrow=<span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">sce <- SingleCellExperiment(counts)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"font-size: 15px;color: rgb(255, 255, 254);line-height: 2;letter-spacing: 2px;padding-right: 15px;padding-left: 15px;font-family: PingFangSC-light;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>2. \u52a0\u5165\u964d\u7ef4\u6570\u636e<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u63a5\u4e0b\u6765\uff0c\u8ba9\u6211\u4eec\u521b\u5efa\u4e00\u4e2a\u5305\u542b\u964d\u7ef4\u6570\u636e\u7684 SingleCellExperiment \u5bf9\u8c61\u3002\u6211\u4eec\u5c06\u4f7f\u7528 PCA \u548c t-SNE \u4f5c\u4e3a\u964d\u7ef4\u65b9\u6cd5\uff0c\u5e76\u5c06\u5b83\u4eec\u5b58\u50a8\u5728 reducedDims \u4e2d\u3002\u4ee5\u4e0b\u662f\u521b\u5efa\u5305\u542b\u964d\u7ef4\u6570\u636e\u7684 SingleCellExperiment \u5bf9\u8c61\u7684\u793a\u4f8b\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">ncells <- 100<\/span><\/code><code><span class=\"code-snippet_outer\">u <- matrix(rpois(<span class=\"code-snippet__number\">20000<\/span>, <span class=\"code-snippet__number\">5<\/span>), ncol=ncells)<\/span><\/code><code><span class=\"code-snippet_outer\">v <- log2(u + <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">pca <- matrix(runif(ncells*<span class=\"code-snippet__number\">5<\/span>), ncells)<\/span><\/code><code><span class=\"code-snippet_outer\">tsne <- matrix(rnorm(ncells*<span class=\"code-snippet__number\">2<\/span>), ncells)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">sce <- SingleCellExperiment(assays=<span class=\"code-snippet__built_in\">list<\/span>(counts=u, logcounts=v),<\/span><\/code><code><span class=\"code-snippet_outer\"> reducedDims=SimpleList(PCA=pca, tSNE=tsne))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"font-size: 15px;color: rgb(255, 255, 254);line-height: 2;letter-spacing: 2px;padding-right: 15px;padding-left: 15px;font-family: PingFangSC-light;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>3. \u4f7f\u7528 SummarizedExperiment \u5bf9\u8c61\u8f6c\u6362<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>SingleCellExperiment \u5305\u8fd8\u53ef\u4ee5\u4f7f\u7528 SummarizedExperiment \u5bf9\u8c61\u8f6c\u6362\u751f\u6210 SingleCellExperiment \u5bf9\u8c61\u3002\u4ee5\u4e0b\u662f\u793a\u4f8b\u4ee3\u7801\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">se <- SummarizedExperiment(assays=<span class=\"code-snippet__built_in\">list<\/span>(counts=u, logcounts=v))<\/span><\/code><code><span class=\"code-snippet_outer\">as(se, <span class=\"code-snippet__string\">\"SingleCellExperiment\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7033582089552238\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_50gRbeDkMR82eiazhzSyxzPUR6sI8Q.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOzOTvgBq6qGj8JNUmezUibWt93Z50gRbeDkMR82eiazhzSyxzPUR6sI8Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"536\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"font-size: 15px;color: rgb(255, 255, 254);line-height: 2;letter-spacing: 2px;padding-right: 15px;padding-left: 15px;font-family: PingFangSC-light;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>4. \u542b\u6709 colData\u3001rowData \u548c metadata<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u6b64\u5916\uff0c\u6211\u4eec\u8fd8\u53ef\u4ee5\u6dfb\u52a0\u5217\u6570\u636e\uff08colData\uff09\u3001\u884c\u6570\u636e\uff08rowData\uff09\u548c\u5143\u6570\u636e\uff08metadata\uff09\u5230 SingleCellExperiment \u5bf9\u8c61\u4e2d\u3002\u4ee5\u4e0b\u662f\u4e00\u4e2a\u793a\u4f8b\uff0c\u5c55\u793a\u5982\u4f55\u521b\u5efa\u5177\u6709\u8fd9\u4e9b\u4fe1\u606f\u7684 SingleCellExperiment \u5bf9\u8c61\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">pretend.cell.labels <- sample(<span class=\"code-snippet__built_in\">letters<\/span>, ncol(counts), replace=<span class=\"code-snippet__literal\">TRUE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pretend.gene.lengths <- sample(<span class=\"code-snippet__number\">10000<\/span>, nrow(counts))<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">sce <- SingleCellExperiment(<span class=\"code-snippet__built_in\">list<\/span>(counts=counts),<\/span><\/code><code><span class=\"code-snippet_outer\"> colData=DataFrame(label=pretend.cell.labels),<\/span><\/code><code><span class=\"code-snippet_outer\"> rowData=DataFrame(<span class=\"code-snippet__built_in\">length<\/span>=pretend.gene.lengths),<\/span><\/code><code><span class=\"code-snippet_outer\"> metadata=<span class=\"code-snippet__built_in\">list<\/span>(study=<span class=\"code-snippet__string\">\"GSE111111\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p><strong>\u63d0\u53d6\u884c\u5217\u4fe1\u606f<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u5728 SingleCellExperiment \u5bf9\u8c61\u4e2d\uff0c\u53ef\u4ee5\u63d0\u53d6\u8868\u8fbe\u77e9\u9635\u3001\u5143\u6570\u636e\u4ee5\u53ca\u5176\u4ed6\u76f8\u5173\u4fe1\u606f\u54e6\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6\u8868\u8fbe\u77e9\u9635\u7684\u7ef4\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">dim<\/span>(assay(sce))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">dim<\/span>(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6\u7279\u5b9a\u7684\u8868\u8fbe\u77e9\u9635<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">assay(sce, <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">assays(sce)[[<span class=\"code-snippet__number\">1<\/span>]]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6\u5217\u6570\u636e\u548c\u884c\u6570\u636e\u7684\u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(colData(sce))<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(rowData(sce))<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6\u5143\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">metadata(sce)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u7279\u5b9a\u7684\u8868\u8fbe\u77e9\u9635\u5c55\u793a\u5982\u4e0b\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.1888888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_AfJFqCtk1WujYEo4rPPzEZrtoKd7gw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOfmyXGf5h21hl195nS0rddvpKAfJFqCtk1WujYEo4rPPzEZrtoKd7gw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u884c\u6570\u636e\u548c\u5217\u6570\u636e\u5982\u4e0b\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.19166666666666668\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_lBG5ASxMFvSu7naxh6EzicLT03orzA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOVwQK9EicaCPuYovVj3pJSahyeBlBG5ASxMFvSu7naxh6EzicLT03orzA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u5143\u6570\u636e\u5c55\u793a\u5982\u4e0b\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6222222222222222\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_cUJtF75YwC2zq5BrztuHQKb4SeA20Q.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXO7Juwz0FERNIG96FvUm8ia3hyBcUJtF75YwC2zq5BrztuHQKb4SeA20Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u4f7f\u7528 Alternative Experiment \u65b9\u6cd5<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>SingleCellExperiment \u5305\u8fd8\u63d0\u4f9b\u4e86 Alternative Experiment \u65b9\u6cd5\uff0c\u7528\u4e8e\u5904\u7406\u4e0d\u540c\u5f62\u5f0f\u7684\u5b9e\u9a8c\u6570\u636e\u3002\u4ee5\u4e0b\u662f\u4e00\u4e9b\u4f7f\u7528\u8fd9\u4e9b\u65b9\u6cd5\u7684\u793a\u4f8b\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u67e5\u770b\u53ef\u7528\u7684 Alternative Experiment \u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">altExpNames(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8bbf\u95ee\u6307\u5b9a\u7684 Alternative Experiment<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">altExp(sce, <span class=\"code-snippet__string\">\"ERCC\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">altExp(sce, <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6 Alternative Experiment \u7684\u8868\u8fbe\u77e9\u9635<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">assay(altExp(sce, <span class=\"code-snippet__string\">\"ERCC\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7287037037037037\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_HHbtE3DoL5rFWOxnjJsHibY53ueEvA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOfoU9jNZ3pPuwvdHasjtCP03aJHHbtE3DoL5rFWOxnjJsHibY53ueEvA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p><strong>\u53d6\u5b50\u96c6<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u5728 SingleCellExperiment \u5bf9\u8c61\u4e2d\uff0c\u6211\u4eec\u8fd8\u53ef\u4ee5\u6839\u636e\u6307\u5b9a\u7684\u884c\u548c\u5217\u6765\u53d6\u5b50\u96c6\u3002\u4ee5\u4e0b\u662f\u793a\u4f8b\u4ee3\u7801\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u4f7f\u7528\u7d22\u5f15\u53d6\u5b50\u96c6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sce[<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">2<\/span>, <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">3<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6839\u636e\u6307\u5b9a\u7684\u884c\u540d\u53d6\u5b50\u96c6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sce[<span class=\"code-snippet__string\">\"A1BG__chr19\"<\/span>, ]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6839\u636e\u6307\u5b9a\u7684\u5217\u540d\u53d6\u5b50\u96c6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sce[, <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"D28-1_1\"<\/span>, <span class=\"code-snippet__string\">\"D28-1_2\"<\/span>)]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6839\u636e rowData \u548c colData \u8fdb\u884c\u9009\u62e9<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u5982\u679c\u6709 NA \u503c\uff0c\u9700\u8981\u53bb\u9664<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sce[, sce$donore == <span class=\"code-snippet__string\">\"D28\"<\/span>]<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u8fd0\u884c\u7ed3\u679c\u5c55\u793a\u5982\u4e0b\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9212962962962963\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_7gOfo67rNyxcw0r8icVOXsuZNnRibQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXO4sYpSknb5JBeE6jgtvcMelqfta7gOfo67rNyxcw0r8icVOXsuZNnRibQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>\u4ee5\u4e0a\u5c31\u662fSingleCellExperiment\u7684\u57fa\u672c\u4f7f\u7528\u65b9\u6cd5\uff0c\u600e\u4e48\u6837\uff0c\u4eca\u5929\u7684\u6559\u7a0b\u4f60\u5b66\u4f1a\u4e86\u561b\uff1f\u662f\u4e0d\u662f\u5f88\u7b80\u5355\u5462\uff01\u66f4\u591a\u5b66\u4e60\u5e72\u8d27\u8981\u7ee7\u7eed\u5173\u6ce8\u5927\u6d77\u54e5\u54e6\uff01<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOYJyjlsib6bQ74Wibtria9gSC4sqocafb5YBQcZWdQRrtP2Xo7vaFWaecA\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_ocafb5YBQcZWdQRrtP2Xo7vaFWaecA-5.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOYJyjlsib6bQ74Wibtria9gSC4sqocafb5YBQcZWdQRrtP2Xo7vaFWaecA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"895\" style=\"\" data-recalc-dims=\"1\" \/><\/span><\/a><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3488888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_07T3ukOr5mxE6sbTMZvYNklUgT3oKg-5.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOYQlIrRTwqxXypnzYht7bhh3T07T3ukOr5mxE6sbTMZvYNklUgT3oKg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<section style=\"border-bottom: 2px solid rgb(0, 150, 136);font-size: 14px;text-align: left;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>\u70b9\u51fb\u201c\u9605\u8bfb\u539f\u6587\u201d\u8fdb\u5165\u7f51\u5740<\/p>\n<\/section>\n<section style=\"margin-left: 8%;margin-right: 8%;line-height: 1.1em;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;vertical-align: top;border-top: 1em solid rgb(0, 150, 136);border-left: 0.8em solid transparent !important;border-right: 0.8em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5355\u7ec6\u80de\u63a2\u79d8\uff1aSingleCellExperiment\u63ed\u5f00\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u7684\u795e\u79d8\u9762\u7eb1\uff01 \u5927\u6d77\u54e5 &nbs […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/56745"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=56745"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/56745\/revisions"}],"predecessor-version":[{"id":56754,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/56745\/revisions\/56754"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=56745"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=56745"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=56745"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

{"id":56745,"date":"2024-06-16T18:19:05","date_gmt":"2024-06-16T10:19:05","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=56745"},"modified":"2024-06-16T18:19:05","modified_gmt":"2024-06-16T10:19:05","slug":"%e5%8d%95%e7%bb%86%e8%83%9e%e6%8e%a2%e7%a7%98%ef%bc%9asinglecellexperiment%e6%8f%ad%e5%bc%80%e5%8d%95%e7%bb%86%e8%83%9e%e8%bd%ac%e5%bd%95%e7%bb%84%e5%ad%a6%e7%9a%84%e7%a5%9e%e7%a7%98%e9%9d%a2%e7%ba%b1","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=56745","title":{"rendered":"\u5355\u7ec6\u80de\u63a2\u79d8\uff1aSingleCellExperiment\u63ed\u5f00\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u7684\u795e\u79d8\u9762\u7eb1\uff01"},"content":{"rendered":"

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\/><\/p>\n<p>hello\uff0c\u4f60\u77e5\u9053\u5982\u4f55\u4f7f\u7528SingleCellExperiment\u5305\u6765\u5206\u6790\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u6570\u636e\u561b\uff1f\u4eca\u5929\u5927\u6d77\u54e5\u5c31\u6765\u6559\u5927\u5bb6\u5982\u4f55\u5177\u4f53\u4f7f\u7528\u8fd9\u4e2aR\u5305\uff0c\u5e72\u8d27\u6ee1\u6ee1\uff0c\u611f\u5174\u8da3\u7684\u8bdd\u5c31\u548c\u5927\u6d77\u54e5\u4e00\u8d77\u770b\u4e0b\u53bb\u5427\uff01<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u8d70\u8fdbSingleCellExperiment<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u9996\u5148\uff0c\u5927\u6d77\u54e5\u6765\u7b80\u5355\u7684\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e0b\u8fd9\u4e2aR\u5305\uff1a<\/p>\n<p>SingleCellExperiment\u5305\u662f\u7528\u4e8e\u5b58\u50a8\u548c\u5206\u6790\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u6570\u636e\u7684R\u8bed\u8a00\u5de5\u5177\u5305\u3002\u5b83\u63d0\u4f9b\u4e86\u4e00\u4e2a\u7075\u6d3b\u7684\u6570\u636e\u7ed3\u6784\uff0c\u53ef\u4ee5\u5bb9\u7eb3\u5355\u7ec6\u80de\u6570\u636e\u4e2d\u7684\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u3001\u5143\u6570\u636e\u548c\u5176\u4ed6\u76f8\u5173\u4fe1\u606f\uff0c\u5e76\u4e3a\u7528\u6237\u63d0\u4f9b\u4e86\u591a\u79cd\u529f\u80fd\u6765\u5904\u7406\u548c\u5206\u6790\u8fd9\u4e9b\u6570\u636e\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>SingleCellExperiment\u5305\u7684\u4e3b\u8981\u7279\u70b9\u5305\u62ec\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"color: rgb(59, 149, 115);\">\u6570\u636e\u5b58\u50a8<\/span><\/strong>\uff1aSingleCellExperiment\u5305\u4f7f\u7528\u4e00\u79cd\u79f0\u4e3aSingleCellExperiment\u7c7b\u7684\u6570\u636e\u7ed3\u6784\u6765\u5b58\u50a8\u5355\u7ec6\u80de\u6570\u636e\u3002\u8be5\u7c7b\u7ee7\u627f\u81eaSummarizedExperiment\u7c7b\uff0c\u56e0\u6b64\u4e5f\u53ef\u4ee5\u4f7f\u7528SummarizedExperiment\u7c7b\u7684\u65b9\u6cd5\u548c\u51fd\u6570\u3002\u8fd9\u4e2a\u6570\u636e\u7ed3\u6784\u65e8\u5728\u7b80\u5316\u5355\u7ec6\u80de\u6570\u636e\u7684\u7ba1\u7406\u548c\u8bbf\u95ee\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"color: rgb(59, 149, 115);\">\u7075\u6d3b\u6027<\/span><\/strong>\uff1aSingleCellExperiment\u5305\u63d0\u4f9b\u4e86\u7075\u6d3b\u7684\u6570\u636e\u5b58\u50a8\u65b9\u5f0f\u3002\u9664\u4e86\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u5916\uff0c\u5b83\u8fd8\u53ef\u4ee5\u5b58\u50a8\u548c\u7ba1\u7406\u964d\u7ef4\u6570\u636e\u3001\u5217\u6570\u636e\u3001\u884c\u6570\u636e\u548c\u5143\u6570\u636e\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"color: rgb(59, 149, 115);\">\u6570\u636e\u5206\u6790<\/span><\/strong>\uff1aSingleCellExperiment\u5305\u63d0\u4f9b\u4e86\u8bb8\u591a\u7528\u4e8e\u5904\u7406\u548c\u5206\u6790\u5355\u7ec6\u80de\u6570\u636e\u7684\u51fd\u6570\u548c\u65b9\u6cd5\u3002\u5b83\u652f\u6301\u5e38\u89c1\u7684\u6570\u636e\u64cd\u4f5c\uff0c\u5982\u5b50\u96c6\u9009\u62e9\u3001\u8fc7\u6ee4\u3001\u6807\u51c6\u5316\u548c\u53d8\u6362\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"color: rgb(59, 149, 115);\">\u53ef\u6269\u5c55\u6027<\/span><\/strong>\uff1aSingleCellExperiment\u5305\u7684\u6570\u636e\u7ed3\u6784\u53ef\u4ee5\u65b9\u4fbf\u5730\u4e0e\u5176\u4ed6\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u5de5\u5177\u548c\u8f6f\u4ef6\u5305\u8fdb\u884c\u4ea4\u4e92\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u603b\u4e4b\uff0cSingleCellExperiment\u5305\u662f\u4e00\u4e2a\u529f\u80fd\u5f3a\u5927\u4e14\u6613\u4e8e\u4f7f\u7528\u7684\u5de5\u5177\u5305\uff0c\u4e13\u95e8\u8bbe\u8ba1\u7528\u4e8e\u5904\u7406\u548c\u5206\u6790\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u6570\u636e\u3002\u5b83\u63d0\u4f9b\u4e86\u7075\u6d3b\u7684\u6570\u636e\u5b58\u50a8\u65b9\u5f0f\u548c\u4e30\u5bcc\u7684\u5206\u6790\u529f\u80fd\uff0c\u4f7f\u7528\u6237\u80fd\u591f\u66f4\u597d\u5730\u7406\u89e3\u548c\u89e3\u91ca\u5355\u7ec6\u80de\u6570\u636e\u3002<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>SingleCellExperiment\u8fdb\u884c\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p><strong>\u5b89\u88c5\u548c\u52a0\u8f7d SingleCellExperiment \u5305<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u9996\u5148\uff0c\u6211\u4eec\u9700\u8981\u786e\u4fdd\u5df2\u5b89\u88c5 SingleCellExperiment \u5305\u3002\u5982\u679c\u5c1a\u672a\u5b89\u88c5\uff0c\u8bf7\u5927\u5bb6\u8fd0\u884c\u4ee5\u4e0b\u4ee3\u7801\u54e6\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span> (!requireNamespace(<span class=\"code-snippet__string\">\"BiocManager\"<\/span>, quietly = <span class=\"code-snippet__literal\">TRUE<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(<span class=\"code-snippet__string\">\"BiocManager\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">BiocManager::install(<span class=\"code-snippet__string\">\"SingleCellExperiment\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u7136\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u52a0\u8f7d SingleCellExperiment \u5305\u4ee5\u5f00\u59cb\u4f7f\u7528\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">library(SingleCellExperiment)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p><strong>\u521b\u5efa SingleCellExperiment \u5bf9\u8c61<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"font-size: 15px;color: rgb(255, 255, 254);line-height: 2;letter-spacing: 2px;padding-right: 15px;padding-left: 15px;font-family: PingFangSC-light;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>1. \u521b\u5efa\u8868\u8fbe\u77e9\u9635<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u9996\u5148\uff0c\u6211\u4eec\u53ef\u4ee5\u521b\u5efa\u4e00\u4e2a\u53ea\u5305\u542b\u8868\u8fbe\u77e9\u9635\u7684 SingleCellExperiment \u5bf9\u8c61\u3002\u5047\u8bbe\u6211\u4eec\u6709\u4e00\u4e2a 10×10 \u7684\u8868\u8fbe\u77e9\u9635\uff0c\u53ef\u4ee5\u4f7f\u7528\u4ee5\u4e0b\u4ee3\u7801\u521b\u5efa\u4e00\u4e2a SingleCellExperiment \u5bf9\u8c61\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">counts <- matrix(rpois(<span class=\"code-snippet__number\">100<\/span>, lambda = <span class=\"code-snippet__number\">10<\/span>), ncol=<span class=\"code-snippet__number\">10<\/span>, nrow=<span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">sce <- SingleCellExperiment(counts)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"font-size: 15px;color: rgb(255, 255, 254);line-height: 2;letter-spacing: 2px;padding-right: 15px;padding-left: 15px;font-family: PingFangSC-light;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>2. \u52a0\u5165\u964d\u7ef4\u6570\u636e<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u63a5\u4e0b\u6765\uff0c\u8ba9\u6211\u4eec\u521b\u5efa\u4e00\u4e2a\u5305\u542b\u964d\u7ef4\u6570\u636e\u7684 SingleCellExperiment \u5bf9\u8c61\u3002\u6211\u4eec\u5c06\u4f7f\u7528 PCA \u548c t-SNE \u4f5c\u4e3a\u964d\u7ef4\u65b9\u6cd5\uff0c\u5e76\u5c06\u5b83\u4eec\u5b58\u50a8\u5728 reducedDims \u4e2d\u3002\u4ee5\u4e0b\u662f\u521b\u5efa\u5305\u542b\u964d\u7ef4\u6570\u636e\u7684 SingleCellExperiment \u5bf9\u8c61\u7684\u793a\u4f8b\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">ncells <- 100<\/span><\/code><code><span class=\"code-snippet_outer\">u <- matrix(rpois(<span class=\"code-snippet__number\">20000<\/span>, <span class=\"code-snippet__number\">5<\/span>), ncol=ncells)<\/span><\/code><code><span class=\"code-snippet_outer\">v <- log2(u + <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">pca <- matrix(runif(ncells*<span class=\"code-snippet__number\">5<\/span>), ncells)<\/span><\/code><code><span class=\"code-snippet_outer\">tsne <- matrix(rnorm(ncells*<span class=\"code-snippet__number\">2<\/span>), ncells)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">sce <- SingleCellExperiment(assays=<span class=\"code-snippet__built_in\">list<\/span>(counts=u, logcounts=v),<\/span><\/code><code><span class=\"code-snippet_outer\"> reducedDims=SimpleList(PCA=pca, tSNE=tsne))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"font-size: 15px;color: rgb(255, 255, 254);line-height: 2;letter-spacing: 2px;padding-right: 15px;padding-left: 15px;font-family: PingFangSC-light;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>3. \u4f7f\u7528 SummarizedExperiment \u5bf9\u8c61\u8f6c\u6362<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>SingleCellExperiment \u5305\u8fd8\u53ef\u4ee5\u4f7f\u7528 SummarizedExperiment \u5bf9\u8c61\u8f6c\u6362\u751f\u6210 SingleCellExperiment \u5bf9\u8c61\u3002\u4ee5\u4e0b\u662f\u793a\u4f8b\u4ee3\u7801\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">se <- SummarizedExperiment(assays=<span class=\"code-snippet__built_in\">list<\/span>(counts=u, logcounts=v))<\/span><\/code><code><span class=\"code-snippet_outer\">as(se, <span class=\"code-snippet__string\">\"SingleCellExperiment\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7033582089552238\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_50gRbeDkMR82eiazhzSyxzPUR6sI8Q.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOzOTvgBq6qGj8JNUmezUibWt93Z50gRbeDkMR82eiazhzSyxzPUR6sI8Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"536\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"font-size: 15px;color: rgb(255, 255, 254);line-height: 2;letter-spacing: 2px;padding-right: 15px;padding-left: 15px;font-family: PingFangSC-light;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>4. \u542b\u6709 colData\u3001rowData \u548c metadata<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u6b64\u5916\uff0c\u6211\u4eec\u8fd8\u53ef\u4ee5\u6dfb\u52a0\u5217\u6570\u636e\uff08colData\uff09\u3001\u884c\u6570\u636e\uff08rowData\uff09\u548c\u5143\u6570\u636e\uff08metadata\uff09\u5230 SingleCellExperiment \u5bf9\u8c61\u4e2d\u3002\u4ee5\u4e0b\u662f\u4e00\u4e2a\u793a\u4f8b\uff0c\u5c55\u793a\u5982\u4f55\u521b\u5efa\u5177\u6709\u8fd9\u4e9b\u4fe1\u606f\u7684 SingleCellExperiment \u5bf9\u8c61\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">pretend.cell.labels <- sample(<span class=\"code-snippet__built_in\">letters<\/span>, ncol(counts), replace=<span class=\"code-snippet__literal\">TRUE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pretend.gene.lengths <- sample(<span class=\"code-snippet__number\">10000<\/span>, nrow(counts))<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">sce <- SingleCellExperiment(<span class=\"code-snippet__built_in\">list<\/span>(counts=counts),<\/span><\/code><code><span class=\"code-snippet_outer\"> colData=DataFrame(label=pretend.cell.labels),<\/span><\/code><code><span class=\"code-snippet_outer\"> rowData=DataFrame(<span class=\"code-snippet__built_in\">length<\/span>=pretend.gene.lengths),<\/span><\/code><code><span class=\"code-snippet_outer\"> metadata=<span class=\"code-snippet__built_in\">list<\/span>(study=<span class=\"code-snippet__string\">\"GSE111111\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p><strong>\u63d0\u53d6\u884c\u5217\u4fe1\u606f<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u5728 SingleCellExperiment \u5bf9\u8c61\u4e2d\uff0c\u53ef\u4ee5\u63d0\u53d6\u8868\u8fbe\u77e9\u9635\u3001\u5143\u6570\u636e\u4ee5\u53ca\u5176\u4ed6\u76f8\u5173\u4fe1\u606f\u54e6\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6\u8868\u8fbe\u77e9\u9635\u7684\u7ef4\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">dim<\/span>(assay(sce))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">dim<\/span>(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6\u7279\u5b9a\u7684\u8868\u8fbe\u77e9\u9635<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">assay(sce, <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">assays(sce)[[<span class=\"code-snippet__number\">1<\/span>]]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6\u5217\u6570\u636e\u548c\u884c\u6570\u636e\u7684\u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(colData(sce))<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(rowData(sce))<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6\u5143\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">metadata(sce)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u7279\u5b9a\u7684\u8868\u8fbe\u77e9\u9635\u5c55\u793a\u5982\u4e0b\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.1888888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_AfJFqCtk1WujYEo4rPPzEZrtoKd7gw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOfmyXGf5h21hl195nS0rddvpKAfJFqCtk1WujYEo4rPPzEZrtoKd7gw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u884c\u6570\u636e\u548c\u5217\u6570\u636e\u5982\u4e0b\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.19166666666666668\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_lBG5ASxMFvSu7naxh6EzicLT03orzA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOVwQK9EicaCPuYovVj3pJSahyeBlBG5ASxMFvSu7naxh6EzicLT03orzA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u5143\u6570\u636e\u5c55\u793a\u5982\u4e0b\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6222222222222222\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_cUJtF75YwC2zq5BrztuHQKb4SeA20Q.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXO7Juwz0FERNIG96FvUm8ia3hyBcUJtF75YwC2zq5BrztuHQKb4SeA20Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u4f7f\u7528 Alternative Experiment \u65b9\u6cd5<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>SingleCellExperiment \u5305\u8fd8\u63d0\u4f9b\u4e86 Alternative Experiment \u65b9\u6cd5\uff0c\u7528\u4e8e\u5904\u7406\u4e0d\u540c\u5f62\u5f0f\u7684\u5b9e\u9a8c\u6570\u636e\u3002\u4ee5\u4e0b\u662f\u4e00\u4e9b\u4f7f\u7528\u8fd9\u4e9b\u65b9\u6cd5\u7684\u793a\u4f8b\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u67e5\u770b\u53ef\u7528\u7684 Alternative Experiment \u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">altExpNames(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8bbf\u95ee\u6307\u5b9a\u7684 Alternative Experiment<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">altExp(sce, <span class=\"code-snippet__string\">\"ERCC\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">altExp(sce, <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6 Alternative Experiment \u7684\u8868\u8fbe\u77e9\u9635<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">assay(altExp(sce, <span class=\"code-snippet__string\">\"ERCC\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7287037037037037\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_HHbtE3DoL5rFWOxnjJsHibY53ueEvA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOfoU9jNZ3pPuwvdHasjtCP03aJHHbtE3DoL5rFWOxnjJsHibY53ueEvA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p><strong>\u53d6\u5b50\u96c6<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u5728 SingleCellExperiment \u5bf9\u8c61\u4e2d\uff0c\u6211\u4eec\u8fd8\u53ef\u4ee5\u6839\u636e\u6307\u5b9a\u7684\u884c\u548c\u5217\u6765\u53d6\u5b50\u96c6\u3002\u4ee5\u4e0b\u662f\u793a\u4f8b\u4ee3\u7801\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u4f7f\u7528\u7d22\u5f15\u53d6\u5b50\u96c6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sce[<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">2<\/span>, <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">3<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6839\u636e\u6307\u5b9a\u7684\u884c\u540d\u53d6\u5b50\u96c6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sce[<span class=\"code-snippet__string\">\"A1BG__chr19\"<\/span>, ]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6839\u636e\u6307\u5b9a\u7684\u5217\u540d\u53d6\u5b50\u96c6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sce[, <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"D28-1_1\"<\/span>, <span class=\"code-snippet__string\">\"D28-1_2\"<\/span>)]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6839\u636e rowData \u548c colData \u8fdb\u884c\u9009\u62e9<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u5982\u679c\u6709 NA \u503c\uff0c\u9700\u8981\u53bb\u9664<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sce[, sce$donore == <span class=\"code-snippet__string\">\"D28\"<\/span>]<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u8fd0\u884c\u7ed3\u679c\u5c55\u793a\u5982\u4e0b\uff1a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9212962962962963\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_7gOfo67rNyxcw0r8icVOXsuZNnRibQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXO4sYpSknb5JBeE6jgtvcMelqfta7gOfo67rNyxcw0r8icVOXsuZNnRibQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>\u4ee5\u4e0a\u5c31\u662fSingleCellExperiment\u7684\u57fa\u672c\u4f7f\u7528\u65b9\u6cd5\uff0c\u600e\u4e48\u6837\uff0c\u4eca\u5929\u7684\u6559\u7a0b\u4f60\u5b66\u4f1a\u4e86\u561b\uff1f\u662f\u4e0d\u662f\u5f88\u7b80\u5355\u5462\uff01\u66f4\u591a\u5b66\u4e60\u5e72\u8d27\u8981\u7ee7\u7eed\u5173\u6ce8\u5927\u6d77\u54e5\u54e6\uff01<\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOYJyjlsib6bQ74Wibtria9gSC4sqocafb5YBQcZWdQRrtP2Xo7vaFWaecA\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_ocafb5YBQcZWdQRrtP2Xo7vaFWaecA-5.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOYJyjlsib6bQ74Wibtria9gSC4sqocafb5YBQcZWdQRrtP2Xo7vaFWaecA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"895\" style=\"\" data-recalc-dims=\"1\" \/><\/span><\/a><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3488888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_07T3ukOr5mxE6sbTMZvYNklUgT3oKg-5.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOYQlIrRTwqxXypnzYht7bhh3T07T3ukOr5mxE6sbTMZvYNklUgT3oKg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<section style=\"border-bottom: 2px solid rgb(0, 150, 136);font-size: 14px;text-align: left;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>\u70b9\u51fb\u201c\u9605\u8bfb\u539f\u6587\u201d\u8fdb\u5165\u7f51\u5740<\/p>\n<\/section>\n<section style=\"margin-left: 8%;margin-right: 8%;line-height: 1.1em;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;vertical-align: top;border-top: 1em solid rgb(0, 150, 136);border-left: 0.8em solid transparent !important;border-right: 0.8em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5355\u7ec6\u80de\u63a2\u79d8\uff1aSingleCellExperiment\u63ed\u5f00\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u7684\u795e\u79d8\u9762\u7eb1\uff01 \u5927\u6d77\u54e5 &nbs […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/56745"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=56745"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/56745\/revisions"}],"predecessor-version":[{"id":56754,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/56745\/revisions\/56754"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=56745"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=56745"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=56745"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}