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decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012817\" data-ratio=\"1.0221465076660987\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_2H7VaT7LKKsVJJAt1Pdpfiabfl5Vag.png?resize=443%2C453\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyv5Zt69WdcXZibRz9fKGian3zlc9STlU6SEspKXOKyPLh3icNK2H7VaT7LKKsVJJAt1Pdpfiabfl5Vag\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"587\" height=\"453 .54998779296875\" width=\"443 .92999267578125\" data-recalc-dims=\"1\"><\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u7ec6\u80de\u5468\u671f\u662f\u4e00\u4e2a\u9ad8\u5ea6\u4fdd\u5b88\u3001\u8fde\u7eed\u7684\u8fc7\u7a0b\uff0c\u63a7\u5236\u7740\u7ec6\u80de\u7684\u5fe0\u5b9e\u590d\u5236\u548c\u5206\u88c2\u3002\u5355\u7ec6\u80de\u6280\u672f\u4f7f\u5f97\u7ec6\u80de\u5468\u671f\u7684\u6d4b\u91cf\u53d8\u5f97\u8d8a\u6765\u8d8a\u7cbe\u786e\uff0c\u7ec6\u80de\u5468\u671f\u65e2\u4f5c\u4e3a\u4e00\u79cd\u611f\u5174\u8da3\u7684\u751f\u7269\u8fc7\u7a0b\uff0c\u53c8\u4f5c\u4e3a\u4e00\u4e2a\u53ef\u80fd\u7684\u6df7\u6742\u56e0\u7d20\u3002\u5c3d\u7ba1\u5176\u6709\u5f88\u5927\u91cd\u8981\u6027\u548c\u4fdd\u5b88\u6027\uff0c\u4f46\u8fd8\u6ca1\u6709\u666e\u904d\u9002\u7528\u7684\u65b9\u6cd5\u53ef\u4ee5\u4ece\u5355\u7ec6\u80de RNA-seq \u6570\u636e\u4e2d\u4ee5\u9ad8\u5206\u8fa8\u7387\u63a8\u65ad\u7ec6\u80de\u5468\u671f\u4e2d\u7684\u4f4d\u7f6e\u3002<\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u76ee\u524d\u6709\u5f88\u591a\u7ec6\u80de\u5468\u671f\u5206\u6790\u8f6f\u4ef6\uff0c\u6bd4\u5982Seurat,Scanpy\u7b49\u3002\u5728\u8fd9\u91cc\uff0c\u5c0f\u679c\u8ddf\u5927\u5bb6\u4ecb\u7ecd\u4e0btricycle\uff0c\u4e00\u4e2a\u5168\u65b0\u53d1\u5e03\u7684\u7ec6\u80de\u5468\u671f\u5206\u6790 R\/Bioconductor \u5305\uff0c\u901a\u8fc7\u5229\u7528\u7ec6\u80de\u5468\u671f\u751f\u7269\u5b66\u7684\u5173\u952e\u7279\u5f81\u3001\u5468\u671f\u51fd\u6570\u4e3b\u6210\u5206\u5206\u6790\u7684\u6570\u5b66\u7279\u6027\u4ee5\u53ca\u8fc1\u79fb\u5b66\u4e60\u7684\u4f7f\u7528\u6765\u5e94\u5bf9\u8fd9\u4e00\u6311\u6218\u3002\u4e3b\u8981\u4f7f\u7528\u56fa\u5b9a\u7684\u53c2\u8003\u6570\u636e\u96c6\u4f30\u8ba1\u7ec6\u80de\u5468\u671f\u5d4c\u5165\uff0c\u5e76\u5c06\u65b0\u6570\u636e\u6295\u5f71\u5230\u8be5\u53c2\u8003\u5d4c\u5165\u4e2d\uff0c\u8fd9\u79cd\u65b9\u6cd5\u514b\u670d\u4e86\u5b66\u4e60\u6570\u636e\u96c6\u76f8\u5173\u5d4c\u5165\u7684\u5173\u952e\u9650\u5236\u3002\u7136\u540e\uff0cTricycle \u6839\u636e\u6570\u636e\u6295\u5f71\u9884\u6d4b\u7ec6\u80de\u5468\u671f\u4e2d\u7684\u7ec6\u80de\u7279\u5b9a\u4f4d\u7f6e\u3002\u597d\u4e86\uff0c\u4ee5\u4e0a\u5c31\u662f\u6709\u5173tricycle\u7684\u524d\u8a00\u4ecb\u7ecd\uff0c\u8bdd\u4e0d\u591a\u8bf4\uff0c\u5feb\u6765\u8ddf\u5c0f\u679c\u4e00\u8d77\u5b66\u4e60\u5427\uff01<\/span> <\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u5bfc\u5165\u6570\u636e<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"powershell\"><code>library(tricycle)<\/span><\/code><code>data<\/span>(neurosphere_example)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u5c06\u5355\u7ec6\u80de\u6570\u636e\u96c6\u6295\u5f71\u5230\u9884\u5148\u5b66\u4e60\u7684\u7ec6\u80de\u5468\u671f\u7a7a\u95f4<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100012859\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-12-05_\u6700\u65b0\u53d1\u5e03\uff01\u7ec6\u80de\u5468\u671f\u5206\u6790\u5305tricycle\uff01\/3_IWicymEetCc6Enf2BkUE4Yz1Ya5e5w.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyuYsqlJnQUJvnS2iciaDibtfbdV0VmHlriaSjYwjFPLp1BLjokHIWicymEetCc6Enf2BkUE4Yz1Ya5e5w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"font-size: 15px;letter-spacing: 0.578px;text-wrap: wrap;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;text-align: center;\"><\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u6295\u5f71\u7684\u7ec6\u80de\u5468\u671f\u7a7a\u95f4\u5c06\u5b58\u50a8\u5728reduceDims\u4e2d\uff0c\u540d\u79f0\u4e3a\u201ctricycleEmbedding\u201d\uff08\u60a8\u53ef\u4ee5\u8bbe\u7f6e\u5176\u4ed6\u5d4c\u5165\u540d\u79f0\uff09\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code>neurosphere_example<-project_cycle_space(neurosphere_example)<\/span><\/code><code>neurosphere_example<\/span><\/code><code>library(ggplot2)<\/span><\/code><code>library(scattermore)<\/span><\/code><code>library(scater)<\/span><\/code><code>scater::plotReducedDim(neurosphere_example, dimred=\"tricycleEmbedding\"<\/span>) +<\/span><\/code><code> labs(x<\/span>=\"Projected PC1\"<\/span>, y<\/span>=\"Projected PC2\"<\/span>) +<\/span><\/code><code> ggtitle(sprintf<\/span>(\"Projected cell cycle space (n=%d)\"<\/span>,<\/span><\/code><code> ncol(neurosphere_example))) +<\/span><\/code><code> theme_bw(base_size=14<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\"> <o:p> <\/o:p><\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012819\" data-ratio=\"0.6148148148148148\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_FtkHdqzzyq5Hib2VUFIJKWwKCTSMgg.png?resize=553%2C340\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyv5Zt69WdcXZibRz9fKGian3CRRMzSTwK8fJXsaV81LkLhFHBFtkHdqzzyq5Hib2VUFIJKWwKCTSMgg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"340 .1400146484375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span> <\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u63a8\u65ad\u7ec6\u80de\u5468\u671f\u4f4d\u7f6e<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>neurosphere_example<-estimate_cycle_position(neurosphere_example)<\/span><\/code><code>names(colData(neurosphere_example))<\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u63a8\u65ad\u7ec6\u80de\u5468\u671f\u9636\u6bb5<\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u6211\u4eec\u8ba1\u7b97\u9ad8\u8868\u8fbe\u7684\u9636\u6bb5\u7279\u5f02\u6027\u7ec6\u80de\u5468\u671f\u6807\u8bb0\u57fa\u56e0\u7684 z \u5206\u6570\uff0c\u5e76\u5c06\u7ec6\u80de\u5206\u914d\u5230\u5177\u6709\u6700\u5927 z \u5206\u6570\u7684\u9636\u6bb5\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>neurosphere_example<-estimate_Schwabe_stage(neurosphere_example,<\/span><\/span><\/span><\/code><code> gname.type<\/span>='ENSEMBL'<\/span>,<\/span><\/code><code> species<\/span>='mouse'<\/span>)<\/span><\/code><code>scater::plotReducedDim<\/span>(neurosphere_example<\/span>, dimred<\/span>=\"tricycleEmbedding\"<\/span>,<\/span><\/code><code> colour_by<\/span>=\"CCStage\"<\/span>) +<\/span><\/code><code> labs<\/span>(x<\/span>=\"Projected PC1\"<\/span>, y<\/span>=\"Projected PC2\"<\/span>,<\/span><\/code><code> title<\/span>=paste0(<\/span>\"Projected<\/span> cell<\/span> cycle<\/span> space<\/span> (n<\/span>=\", ncol(neurosphere_example), \"<\/span>)\")) +<\/span><\/code><code> theme_bw<\/span>(base_size<\/span>=14)<\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012820\" data-ratio=\"0.6231481481481481\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-12-05_\u6700\u65b0\u53d1\u5e03\uff01\u7ec6\u80de\u5468\u671f\u5206\u6790\u5305tricycle\uff01\/5_ibFC6V2qfBQaVMBNnpbj1V9wl35xZw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyv5Zt69WdcXZibRz9fKGian3jIpsXhBC0p51Wic1Dr1rBA1ib9ZibFC6V2qfBQaVMBNnpbj1V9wl35xZw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"344.66998291015625\" width=\"553.739990234375\" \/><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u6839\u636e\u7ec6\u80de\u5468\u671f\u4f4d\u7f6e\u7ed8\u5236\u5d4c\u5165\u6563\u70b9\u56fe<\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u4e3a\u4e86\u53ef\u89c6\u5316\u4efb\u4f55\u5d4c\u5165\u4e0a\u7684\u7ec6\u80de\u5468\u671f\u4f4d\u7f6e \u03b8\uff0c\u6211\u4eec\u9700\u8981\u4ed4\u7ec6\u9009\u62e9\u5faa\u73af\u8c03\u8272\u677f\u3002\u56e0\u6b64\uff0c\u6211\u4eec\u5305\u542b\u8fd9\u6837\u7684\u51fd\u6570\u6765\u7ed8\u5236\u5177\u6709\u5faa\u73af\u53d8\u91cf\u7684 SingleCellExperiment \u5bf9\u8c61\u7684\u4efb\u4f55\u5d4c\u5165\u3002\u8fd8\u53ef\u4ee5\u4f7f\u7528\u7528\u4e8e\u521b\u5efa\u5faa\u73af\u56fe\u4f8b\u7684\u914d\u5957\u8f85\u52a9\u51fd\u6570\u3002<\/span> <\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"swift\"><code>library(cowplot)<\/span><\/code><code>p<-plot_emb_circle_scale(neurosphere_example, dimred=1<\/span>,<\/span><\/code><code> point.size=3.5<\/span>, point.alpha=0.9<\/span>) +<\/span><\/code><code> theme_bw(base_size=14<\/span>)<\/span><\/code><code>legend<-circle_scale_legend(text.size=5<\/span>, alpha=0.9<\/span>)<\/span><\/code><code>plot_grid(p, legend, ncol=2<\/span>, rel_widths=c<\/span>(1<\/span>, 0.4<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012818\" data-ratio=\"0.6898148148148148\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_Q2qGzGmiaaZa1nwRQQquApgWQF4Nrw.png?resize=553%2C382\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyv5Zt69WdcXZibRz9fKGian38gxPe3mEibjI7ma9gq6foia3Ze2Q2qGzGmiaaZa1nwRQQquApgWQF4Nrw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"382 .21002197265625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;text-indent: 0pt;letter-spacing: 0.034em;font-family: \u7b49\u7ebf;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: 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onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxPqeNQsFjm2HibIuSld9Nh9GicQSWhJwHjia5tEevQvDIebLErVmRkv7tSNKliafDnsuFR7BoeDgBrww\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"900\" style=\"font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: var(--articleFontsize);letter-spacing: 0.034em;text-align: justify;\" \/><\/p>\n<p style=\"margin-top: 0pt;margin-bottom: 0pt;margin-left: 0pt;letter-spacing: 0.578px;text-indent: 0pt;text-wrap: wrap;text-align: justify;line-height: normal;font-size: 12pt;font-family: helvetica;color: rgb(0, 0, 0);break-after: auto;\"><\/span><\/span><span style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;color: rgba(0, 0, 0, 0.9);font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft 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href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493633&idx=1&sn=2263dfe7572c0bcf6816c2c1a37d79de&chksm=cf7f78c4f808f1d2209321d6ace39fde5fa60c0691b56f88eac21916f5a2872ce8ee345f1d5f&scene=21#wechat_redirect\" textvalue=\"\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;letter-spacing: 0.578px;font-size: 14px;\" rel=\"noopener\">5.\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01<\/a><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<mp-style-type 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{"id":56850,"date":"2024-06-16T18:25:25","date_gmt":"2024-06-16T10:25:25","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=56850"},"modified":"2024-06-16T18:25:25","modified_gmt":"2024-06-16T10:25:25","slug":"%e6%9c%80%e6%96%b0%e5%8f%91%e5%b8%83%ef%bc%81%e7%bb%86%e8%83%9e%e5%91%a8%e6%9c%9f%e5%88%86%e6%9e%90%e5%8c%85tricycle%ef%bc%81-3","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=56850","title":{"rendered":"\u6700\u65b0\u53d1\u5e03\uff01\u7ec6\u80de\u5468\u671f\u5206\u6790\u5305tricycle\uff01"},"content":{"rendered":"

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href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2812010014583390210#wechat_redirect\" title=\"R\u4ee3\u7801\" target=\"_blank\" rel=\"noopener\">#R\u4ee3\u7801<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2821072943068839938#wechat_redirect\" title=\"\u751f\u4fe1\u7ed8\u56fe\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u7ed8\u56fe<\/a><\/p>\n<\/div>\n<p style=\"margin-left: 0pt;text-indent: 0pt;line-height: normal;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;margin-top: 18pt;margin-bottom: 18pt;text-align: justify;background: rgb(255, 255, 255);\" data-mpa-powered-by=\"yiban.io\"><span style=\"text-align: left;text-indent: 0pt;letter-spacing: 0.034em;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;color: rgb(32, 33, 34);background-image: initial;background-position: initial;background-size: initial;background-repeat: initial;background-attachment: initial;background-origin: initial;background-clip: initial;font-size: 15px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100012858\" data-ratio=\"0.23333333333333334\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_rE2v0O5WiaUDJjgv5bxK2G1ePHyH2g-49.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAysHY49vPGCraibakiaNq6hQgn8C5MfvVLQGxlNZPEia2PajKAzrE2v0O5WiaUDJjgv5bxK2G1ePHyH2g\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"font-family: Cambria;font-size: 15px;text-wrap: wrap;letter-spacing: 0.578px;text-align: center;\" data-recalc-dims=\"1\">\u7ec6\u80de\u5468\u671f\uff08cell cycle\uff09\uff0c\u662f\u6307\u80fd\u6301\u7eed<\/span>\u5206\u88c2<\/span><span style=\"text-align: left;text-indent: 0pt;letter-spacing: 0.034em;font-variant-numeric: 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decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012817\" data-ratio=\"1.0221465076660987\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_2H7VaT7LKKsVJJAt1Pdpfiabfl5Vag.png?resize=443%2C453\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyv5Zt69WdcXZibRz9fKGian3zlc9STlU6SEspKXOKyPLh3icNK2H7VaT7LKKsVJJAt1Pdpfiabfl5Vag\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"587\" height=\"453 .54998779296875\" width=\"443 .92999267578125\" data-recalc-dims=\"1\"><\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u7ec6\u80de\u5468\u671f\u662f\u4e00\u4e2a\u9ad8\u5ea6\u4fdd\u5b88\u3001\u8fde\u7eed\u7684\u8fc7\u7a0b\uff0c\u63a7\u5236\u7740\u7ec6\u80de\u7684\u5fe0\u5b9e\u590d\u5236\u548c\u5206\u88c2\u3002\u5355\u7ec6\u80de\u6280\u672f\u4f7f\u5f97\u7ec6\u80de\u5468\u671f\u7684\u6d4b\u91cf\u53d8\u5f97\u8d8a\u6765\u8d8a\u7cbe\u786e\uff0c\u7ec6\u80de\u5468\u671f\u65e2\u4f5c\u4e3a\u4e00\u79cd\u611f\u5174\u8da3\u7684\u751f\u7269\u8fc7\u7a0b\uff0c\u53c8\u4f5c\u4e3a\u4e00\u4e2a\u53ef\u80fd\u7684\u6df7\u6742\u56e0\u7d20\u3002\u5c3d\u7ba1\u5176\u6709\u5f88\u5927\u91cd\u8981\u6027\u548c\u4fdd\u5b88\u6027\uff0c\u4f46\u8fd8\u6ca1\u6709\u666e\u904d\u9002\u7528\u7684\u65b9\u6cd5\u53ef\u4ee5\u4ece\u5355\u7ec6\u80de RNA-seq \u6570\u636e\u4e2d\u4ee5\u9ad8\u5206\u8fa8\u7387\u63a8\u65ad\u7ec6\u80de\u5468\u671f\u4e2d\u7684\u4f4d\u7f6e\u3002<\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u76ee\u524d\u6709\u5f88\u591a\u7ec6\u80de\u5468\u671f\u5206\u6790\u8f6f\u4ef6\uff0c\u6bd4\u5982Seurat,Scanpy\u7b49\u3002\u5728\u8fd9\u91cc\uff0c\u5c0f\u679c\u8ddf\u5927\u5bb6\u4ecb\u7ecd\u4e0btricycle\uff0c\u4e00\u4e2a\u5168\u65b0\u53d1\u5e03\u7684\u7ec6\u80de\u5468\u671f\u5206\u6790 R\/Bioconductor \u5305\uff0c\u901a\u8fc7\u5229\u7528\u7ec6\u80de\u5468\u671f\u751f\u7269\u5b66\u7684\u5173\u952e\u7279\u5f81\u3001\u5468\u671f\u51fd\u6570\u4e3b\u6210\u5206\u5206\u6790\u7684\u6570\u5b66\u7279\u6027\u4ee5\u53ca\u8fc1\u79fb\u5b66\u4e60\u7684\u4f7f\u7528\u6765\u5e94\u5bf9\u8fd9\u4e00\u6311\u6218\u3002\u4e3b\u8981\u4f7f\u7528\u56fa\u5b9a\u7684\u53c2\u8003\u6570\u636e\u96c6\u4f30\u8ba1\u7ec6\u80de\u5468\u671f\u5d4c\u5165\uff0c\u5e76\u5c06\u65b0\u6570\u636e\u6295\u5f71\u5230\u8be5\u53c2\u8003\u5d4c\u5165\u4e2d\uff0c\u8fd9\u79cd\u65b9\u6cd5\u514b\u670d\u4e86\u5b66\u4e60\u6570\u636e\u96c6\u76f8\u5173\u5d4c\u5165\u7684\u5173\u952e\u9650\u5236\u3002\u7136\u540e\uff0cTricycle \u6839\u636e\u6570\u636e\u6295\u5f71\u9884\u6d4b\u7ec6\u80de\u5468\u671f\u4e2d\u7684\u7ec6\u80de\u7279\u5b9a\u4f4d\u7f6e\u3002\u597d\u4e86\uff0c\u4ee5\u4e0a\u5c31\u662f\u6709\u5173tricycle\u7684\u524d\u8a00\u4ecb\u7ecd\uff0c\u8bdd\u4e0d\u591a\u8bf4\uff0c\u5feb\u6765\u8ddf\u5c0f\u679c\u4e00\u8d77\u5b66\u4e60\u5427\uff01<\/span> <\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u5bfc\u5165\u6570\u636e<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"powershell\"><code>library(tricycle)<\/span><\/code><code>data<\/span>(neurosphere_example)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u5c06\u5355\u7ec6\u80de\u6570\u636e\u96c6\u6295\u5f71\u5230\u9884\u5148\u5b66\u4e60\u7684\u7ec6\u80de\u5468\u671f\u7a7a\u95f4<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100012859\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-12-05_\u6700\u65b0\u53d1\u5e03\uff01\u7ec6\u80de\u5468\u671f\u5206\u6790\u5305tricycle\uff01\/3_IWicymEetCc6Enf2BkUE4Yz1Ya5e5w.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyuYsqlJnQUJvnS2iciaDibtfbdV0VmHlriaSjYwjFPLp1BLjokHIWicymEetCc6Enf2BkUE4Yz1Ya5e5w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"font-size: 15px;letter-spacing: 0.578px;text-wrap: wrap;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;text-align: center;\"><\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u6295\u5f71\u7684\u7ec6\u80de\u5468\u671f\u7a7a\u95f4\u5c06\u5b58\u50a8\u5728reduceDims\u4e2d\uff0c\u540d\u79f0\u4e3a\u201ctricycleEmbedding\u201d\uff08\u60a8\u53ef\u4ee5\u8bbe\u7f6e\u5176\u4ed6\u5d4c\u5165\u540d\u79f0\uff09\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code>neurosphere_example<-project_cycle_space(neurosphere_example)<\/span><\/code><code>neurosphere_example<\/span><\/code><code>library(ggplot2)<\/span><\/code><code>library(scattermore)<\/span><\/code><code>library(scater)<\/span><\/code><code>scater::plotReducedDim(neurosphere_example, dimred=\"tricycleEmbedding\"<\/span>) +<\/span><\/code><code> labs(x<\/span>=\"Projected PC1\"<\/span>, y<\/span>=\"Projected PC2\"<\/span>) +<\/span><\/code><code> ggtitle(sprintf<\/span>(\"Projected cell cycle space (n=%d)\"<\/span>,<\/span><\/code><code> ncol(neurosphere_example))) +<\/span><\/code><code> theme_bw(base_size=14<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\"> <o:p> <\/o:p><\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012819\" data-ratio=\"0.6148148148148148\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_FtkHdqzzyq5Hib2VUFIJKWwKCTSMgg.png?resize=553%2C340\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyv5Zt69WdcXZibRz9fKGian3CRRMzSTwK8fJXsaV81LkLhFHBFtkHdqzzyq5Hib2VUFIJKWwKCTSMgg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"340 .1400146484375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span> <\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u63a8\u65ad\u7ec6\u80de\u5468\u671f\u4f4d\u7f6e<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>neurosphere_example<-estimate_cycle_position(neurosphere_example)<\/span><\/code><code>names(colData(neurosphere_example))<\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u63a8\u65ad\u7ec6\u80de\u5468\u671f\u9636\u6bb5<\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u6211\u4eec\u8ba1\u7b97\u9ad8\u8868\u8fbe\u7684\u9636\u6bb5\u7279\u5f02\u6027\u7ec6\u80de\u5468\u671f\u6807\u8bb0\u57fa\u56e0\u7684 z \u5206\u6570\uff0c\u5e76\u5c06\u7ec6\u80de\u5206\u914d\u5230\u5177\u6709\u6700\u5927 z \u5206\u6570\u7684\u9636\u6bb5\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>neurosphere_example<-estimate_Schwabe_stage(neurosphere_example,<\/span><\/span><\/span><\/code><code> gname.type<\/span>='ENSEMBL'<\/span>,<\/span><\/code><code> species<\/span>='mouse'<\/span>)<\/span><\/code><code>scater::plotReducedDim<\/span>(neurosphere_example<\/span>, dimred<\/span>=\"tricycleEmbedding\"<\/span>,<\/span><\/code><code> colour_by<\/span>=\"CCStage\"<\/span>) +<\/span><\/code><code> labs<\/span>(x<\/span>=\"Projected PC1\"<\/span>, y<\/span>=\"Projected PC2\"<\/span>,<\/span><\/code><code> title<\/span>=paste0(<\/span>\"Projected<\/span> cell<\/span> cycle<\/span> space<\/span> (n<\/span>=\", ncol(neurosphere_example), \"<\/span>)\")) +<\/span><\/code><code> theme_bw<\/span>(base_size<\/span>=14)<\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012820\" data-ratio=\"0.6231481481481481\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-12-05_\u6700\u65b0\u53d1\u5e03\uff01\u7ec6\u80de\u5468\u671f\u5206\u6790\u5305tricycle\uff01\/5_ibFC6V2qfBQaVMBNnpbj1V9wl35xZw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyv5Zt69WdcXZibRz9fKGian3jIpsXhBC0p51Wic1Dr1rBA1ib9ZibFC6V2qfBQaVMBNnpbj1V9wl35xZw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"344.66998291015625\" width=\"553.739990234375\" \/><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u6839\u636e\u7ec6\u80de\u5468\u671f\u4f4d\u7f6e\u7ed8\u5236\u5d4c\u5165\u6563\u70b9\u56fe<\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\">\u4e3a\u4e86\u53ef\u89c6\u5316\u4efb\u4f55\u5d4c\u5165\u4e0a\u7684\u7ec6\u80de\u5468\u671f\u4f4d\u7f6e \u03b8\uff0c\u6211\u4eec\u9700\u8981\u4ed4\u7ec6\u9009\u62e9\u5faa\u73af\u8c03\u8272\u677f\u3002\u56e0\u6b64\uff0c\u6211\u4eec\u5305\u542b\u8fd9\u6837\u7684\u51fd\u6570\u6765\u7ed8\u5236\u5177\u6709\u5faa\u73af\u53d8\u91cf\u7684 SingleCellExperiment \u5bf9\u8c61\u7684\u4efb\u4f55\u5d4c\u5165\u3002\u8fd8\u53ef\u4ee5\u4f7f\u7528\u7528\u4e8e\u521b\u5efa\u5faa\u73af\u56fe\u4f8b\u7684\u914d\u5957\u8f85\u52a9\u51fd\u6570\u3002<\/span> <\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"swift\"><code>library(cowplot)<\/span><\/code><code>p<-plot_emb_circle_scale(neurosphere_example, dimred=1<\/span>,<\/span><\/code><code> point.size=3.5<\/span>, point.alpha=0.9<\/span>) +<\/span><\/code><code> theme_bw(base_size=14<\/span>)<\/span><\/code><code>legend<-circle_scale_legend(text.size=5<\/span>, alpha=0.9<\/span>)<\/span><\/code><code>plot_grid(p, legend, ncol=2<\/span>, rel_widths=c<\/span>(1<\/span>, 0.4<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012818\" data-ratio=\"0.6898148148148148\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_Q2qGzGmiaaZa1nwRQQquApgWQF4Nrw.png?resize=553%2C382\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyv5Zt69WdcXZibRz9fKGian38gxPe3mEibjI7ma9gq6foia3Ze2Q2qGzGmiaaZa1nwRQQquApgWQF4Nrw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"382 .21002197265625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: \u5b8b\u4f53;mso-fareast-font-family: '\u5b8b\u4f53';mso-bidi-font-family: \u5b8b\u4f53;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;text-indent: 0pt;letter-spacing: 0.034em;font-family: \u7b49\u7ebf;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;\">\u597d\u4e86\uff0c\u4ee5\u4e0a\u5c31\u662f\u5c0f\u679c\u63a8\u8350\u7684R\u5305tricylce\uff0c\u662f\u4e0d\u662f\u5f88\u7b80\u5355\uff1f\u53ea\u9700\u8981\u7b80\u5355\u7684\u51e0\u884c\u4ee3\u7801\u5c31\u53ef\u4ee5\u7ed8\u5236\u51fa\u5982\u6b64\u7cbe\u7f8e\u7684\u7ec6\u80de\u5468\u671f\u56fe\u3002\u8fd8\u7b49\u4ec0\u4e48\u5462\uff1f\u5feb\u6765\u5b66\u4e60\u5427\uff01\u5173\u6ce8\u751f\u4fe1\u679c\uff0c\u4e00\u8d77\u6765\u5b66\u4e60\u6700\u8fd1\u7684\u751f\u4fe1\u5de5\u5177\uff01<\/span> <\/span><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100012822\" data-ratio=\"0.5555555555555556\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2023-12-05_\u6700\u65b0\u53d1\u5e03\uff01\u7ec6\u80de\u5468\u671f\u5206\u6790\u5305tricycle\uff01\/7_VmRkv7tSNKliafDnsuFR7BoeDgBrww.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxPqeNQsFjm2HibIuSld9Nh9GicQSWhJwHjia5tEevQvDIebLErVmRkv7tSNKliafDnsuFR7BoeDgBrww\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"900\" style=\"font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: var(--articleFontsize);letter-spacing: 0.034em;text-align: justify;\" \/><\/p>\n<p style=\"margin-top: 0pt;margin-bottom: 0pt;margin-left: 0pt;letter-spacing: 0.578px;text-indent: 0pt;text-wrap: wrap;text-align: justify;line-height: normal;font-size: 12pt;font-family: helvetica;color: rgb(0, 0, 0);break-after: auto;\"><\/span><\/span><span style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;color: rgba(0, 0, 0, 0.9);font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;text-align: justify;\"><\/span><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012821\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_FbEg9fibwQicL8l2ExKOZZTmAHXabQ-14.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAx3HetBkB7aOGibFWKqibqIATyC4zFibzCgdrxFwRep6cDMLIbA0FbEg9fibwQicL8l2ExKOZZTmAHXabQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;caret-color: rgba(0, 0, 0, 0);\" data-recalc-dims=\"1\"><\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 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