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15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 29.9652%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 29.9652% !important;\">\n<section style=\"margin-top: 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\/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section style=\"display: contents;\" powered-by=\"xiumi.us\">\n<section style=\"display: contents;\">\n<section style=\"display: contents;\">\n<section style=\"height: 0px;overflow: hidden;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;caret-color: red;\"> <\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<p><span style=\"font-size: 14px;\">\u4e0a\u4e00\u8282\uff0c\u5c0f\u679c\u5411\u5927\u5bb6\u4ecb\u7ecd\u4e86DESeq2\u5dee\u5f02\u5206\u6790\u7684\u65b9\u6cd5\uff0cDESeq2\u5305\u4e5f\u5185\u7f6e\u4e00\u4e9b\u7b80\u5355\u7684\u53ef\u89c6\u5316\u51fd\u6570\uff0c\u53ea\u80fd\u6ee1\u8db3\u6211\u4eec\u7684\u4e00\u4e9b\u57fa\u7840\u9700\u6c42\uff0c\u5982\u4f55\u5c06\u7ed3\u679c\u66f4\u4e30\u5bcc\u7f8e\u89c2\u5730\u8fdb\u884c\u53ef\u89c6\u5316\u5448\u73b0\u5462\uff1f\u706b\u5c71\u56fe\uff08Volcano plot\uff09\u548c\u70ed\u56fe\uff08Heatmap\uff09\u662f\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u4e2d\u5e38\u7528\u7684\u6570\u636e\u53ef\u89c6\u5316\u5de5\u5177\uff0c\u5b83\u4eec\u5728\u7ed3\u679c\u89e3\u91ca\u548c\u6570\u636e\u63a2\u7d22\u4e2d\u8d77\u7740\u91cd\u8981\u7684\u4f5c\u7528\u3002\u4e0b\u9762\u63a5\u7740\u8ddf\u5c0f\u679c\u4e00\u8d77\u6765\u5b66\u4e60\u5427\uff01<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 20px;\"><em><strong>1.\u706b\u5c71\u56fe<\/strong><\/em><\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u706b\u5c71\u56fe\u662f\u4e00\u79cd\u6563\u70b9\u56fe\uff0c\u7528\u4e8e\u53ef\u89c6\u5316\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u7edf\u8ba1\u663e\u8457\u6027\u548c\u751f\u7269\u5b66\u91cd\u8981\u6027\u3002\u5728\u706b\u5c71\u56fe\u4e2d\uff0c\u6a2a\u8f74\u8868\u793a\u57fa\u56e0\u7684\u5dee\u5f02\u8868\u8fbe\u6c34\u5e73\uff08\u5982\u6298\u53e0\u53d8\u5316\u6216\u5bf9\u6570\u53d8\u5316\uff09\uff0c\u7eb5\u8f74\u8868\u793a\u57fa\u56e0\u7684\u7edf\u8ba1\u663e\u8457\u6027\uff08\u5982\u8c03\u6574\u7684 p-value\uff09\u3002\u6bcf\u4e2a\u57fa\u56e0\u4ee5\u4e00\u4e2a\u70b9\u7684\u5f62\u5f0f\u8868\u793a\uff0c\u70b9\u7684\u4f4d\u7f6e\u53d6\u51b3\u4e8e\u57fa\u56e0\u7684\u5dee\u5f02\u8868\u8fbe\u7a0b\u5ea6\u548c\u7edf\u8ba1\u663e\u8457\u6027\u3002\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u901a\u5e38\u4f4d\u4e8e\u56fe\u50cf\u7684\u9876\u90e8\u6216\u5e95\u90e8\uff0c\u800c\u975e\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u5219\u4f4d\u4e8e\u56fe\u50cf\u7684\u4e2d\u592e\u3002\u901a\u8fc7\u89c2\u5bdf\u706b\u5c71\u56fe\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u5feb\u901f\u8bc6\u522b\u51fa\u5dee\u5f02\u8868\u8fbe\u6c34\u5e73\u663e\u8457\u7684\u57fa\u56e0\uff0c\u5e76\u4e86\u89e3\u5b83\u4eec\u7684\u751f\u7269\u5b66\u91cd\u8981\u6027\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u9996\u5148\u6211\u4eec\u9700\u8981\u73b0\u8bbe\u7f6e\u7b5b\u9009\u9608\u503c\uff0c\u5bf9\u4e8e\u5dee\u5f02\u5206\u6790\u7684\u7ed3\u679c\u6765\u8bf4\uff0c\u6709\u4e24\u4e2a\u6bd4\u8f83\u91cd\u8981\u7684\u9608\u503c\uff0c\u5206\u522b\u662f\u77eb\u6b63\u540e\u7684P\u503c\u548clog2FoldChange\u503c\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u77eb\u6b63\u540e\u7684P\u503c\u5c5e\u4e8e\u7edf\u8ba1\u503c\u9608\u503c\uff0c\u5728\u5dee\u5f02\u5206\u6790\u7684\u8fc7\u7a0b\u4e2d\u4f1a\u91c7\u7528\u516c\u8ba4\u7684Benjamini-Hochberg\u6821\u6b63\u65b9\u6cd5\u5bf9\u539f\u6709\u5047\u8bbe\u68c0\u9a8c\u5f97\u5230\u7684\u663e\u8457\u6027p\u503c\u8fdb\u884c\u6821\u6b63\uff0c\u4e00\u822c\u9009\u62e90.01\u6216\u80050.05\u4f5c\u4e3a\u6807\u51c6\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">log2FoldChange\u503c\u662f\u8868\u8fbe\u91cf\u7684\u9608\u503c\uff0c\u8fd9\u4e2a\u503c\u4ee3\u8868\u4e24\u7ec4\u6837\u672c\u95f4\u8868\u8fbe\u91cf\u7684\u6bd4\u503c\uff0c\u4e00\u822c\u5c06\u5dee\u5f02\u57fa\u56e0\u7684\u7b5b\u9009\u6807\u51c6\u8bbe\u7f6e\u4e3alog2FoldChange\u7edd\u5bf9\u503c\u5927\u4e8e1\u6216\u80052\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u8fd9\u4e24\u4e2a\u6307\u6807\u7684\u9009\u53d6\u90fd\u662f\u6839\u636e\u7ecf\u9a8c\u53bb\u8fdb\u884c\u8bbe\u7f6e\uff0c\u5e76\u4e0d\u662f\u5b8c\u5168\u56fa\u5b9a\uff0c\u5728\u5177\u4f53\u5206\u6790\u8fc7\u7a0b\u4e2d\uff0c\u6211\u4eec\u901a\u5e38\u9700\u8981\u6839\u636e\u5f97\u5230\u7684\u5dee\u5f02\u57fa\u56e0\u6570\u76ee\u8fdb\u884c\u5fae\u8c03\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u4ee3\u7801\u5982\u4e0b\uff1a<\/span><\/p>\n<section powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">##\u8bbe\u7f6e\u7b5b\u9009\u9608\u503c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">logFoldChange = 2<\/span><\/code><code><span class=\"code-snippet_outer\">adjustP = 0.05<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">diff_res <- as.data.frame(res)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">diff_res[is.na(diff_res)] <- 0<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u5728\u706b\u5c71\u56fe\u4e2d\u6211\u4eec\u53ef\u4ee5\u770b\u5230\uff0c\u7070\u8272\u7684\u7ebf\u5c31\u4ee3\u8868\u7740\u8fd9\u4e24\u4e2a\u6307\u6807\u7684\u9608\u503c\u3002\u8fd9\u91cc\u6211\u4eec\u5c06P\u503c\u5c0f\u4e8e0.05\u5e76\u4e14log2FoldChange\u503c\u5927\u4e8e2\u7684\u57fa\u56e0\u5b9a\u4e49\u4e3a\u4e0a\u8c03\u57fa\u56e0\uff0c\u5c06P\u503c\u5c0f\u4e8e0.05\u5e76\u4e14log2FoldChange\u503c\u5c0f\u4e8e-2\u7684\u57fa\u56e0\u5b9a\u4e49\u4e3a\u4e0b\u8c03\u57fa\u56e0\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"white-space:pre-wrap;font-family: Consolas, "Liberation Mono", Menlo, Courier, monospace;font-size: 14px;background-color: rgb(250, 250, 250);color: rgb(51, 51, 51);letter-spacing: 0.034em;\">\u200d<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\">diff_res$change <- ifelse(diff_res$padj > adjustP,'stable',<\/span><\/code><code><span class=\"code-snippet_outer\"> ifelse(diff_res$log2FoldChange > logFoldChange,'UP',<\/span><\/code><code><span class=\"code-snippet_outer\"> ifelse(diff_res$log2FoldChange < -logFoldChange,'DOWN','stable'))<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\u6211\u4eec\u5bfc\u5165\u7ed8\u56fe\u6240\u7528R\u5305ggplot2\uff0c\u8bbe\u7f6e\u56fe\u7247\u540d\u79f0\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code><span class=\"code-snippet_outer\">#\u753b\u706b\u5c71\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"># \u5bfc\u5165\u7ed8\u56fe\u5305<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">title <- paste(\"DEGs Volcano Plot\", sep = \"\", collapse = \"\")<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u5bf9\u5e94\u8bbe\u7f6e\u7ed3\u679c\u6570\u636e\uff0cx\u8f74\u4e0ey\u8f74\u6240\u7ed8\u5236\u7684\u6570\u636e\uff0c\u6839\u636e\u4e0a\u4e00\u6b65\u6240\u5b9a\u4e49\u7684\u4e0a\u4e0b\u8c03\u57fa\u56e0\u6765\u7ed8\u5236\u70b9\u7684\u989c\u8272\u3002geom_hline\u4e0egeom_vline\u51fd\u6570\u7528\u4e8e\u7ed8\u5236\u6c34\u5e73\u4e0e\u5782\u76f4\u7684\u8f85\u52a9\u7ebf\u3002<span style=\"font-family: \u7b49\u7ebf;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/span><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code><span class=\"code-snippet_outer\">ggplot(data=diff_res, aes(x=log2FoldChange, y=-log10(padj), color=change)) + <\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(alpha=0.4, size=1.75) +<\/span><\/code><code><span class=\"code-snippet_outer\"> theme_set(theme_set(theme_bw(base_size=20))) +<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab(\"log2 fold change\") + ylab(\"-log10 adj.P.Val\") +<\/span><\/code><code><span class=\"code-snippet_outer\"> ggtitle(title) + theme(plot.title = element_text(size=15,hjust = 0.5)) +<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_colour_manual(values = c('#FF9999','gray80','#99CC00')) + #\u81ea\u5b9a\u4e49\u989c\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_hline(yintercept = c(-log10(adjustP)),linewidth = 1, color = \"grey\", lty = \"dashed\") +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_vline(xintercept = c(-2,2),linewidth = 1, color = \"grey\", lty = \"dashed\") # \u9608\u503c\u8f85\u52a9\u7ebf<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7489177489177489\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_T338otxFib1Oo0xuIHRgDFLVuTjguQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxkQdNGeWtDGfzAUzolwgtQOJzLia4RDYwxFOer5avr3DMrboT338otxFib1Oo0xuIHRgDFLVuTjguQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;font-size: 16px;\"><br \/><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 20px;\"><em><strong>2.\u70ed\u56fe<\/strong><\/em><\/span><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u70ed\u56fe\u662f\u4e00\u79cd\u77e9\u9635\u5f0f\u7684\u56fe\u5f62\u8868\u793a\u65b9\u6cd5\uff0c\u7528\u4e8e\u53ef\u89c6\u5316\u57fa\u56e0\u7684\u8868\u8fbe\u6a21\u5f0f\u3002\u5728\u70ed\u56fe\u4e2d\uff0c\u6bcf\u884c\u4ee3\u8868\u4e00\u4e2a\u57fa\u56e0\uff0c\u6bcf\u5217\u4ee3\u8868\u4e00\u4e2a\u6837\u672c\u3002\u901a\u8fc7\u5bf9\u57fa\u56e0\u8868\u8fbe\u91cf\u8fdb\u884c\u5f52\u4e00\u5316\u548c\u805a\u7c7b\uff0c\u70ed\u56fe\u5c55\u793a\u4e86\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u7684\u76f8\u4f3c\u6027\u548c\u5dee\u5f02\u6027\u3002\u70ed\u56fe\u4e2d\u7684\u989c\u8272\u7f16\u7801\u8868\u793a\u57fa\u56e0\u8868\u8fbe\u91cf\u7684\u9ad8\u4f4e\uff0c\u901a\u5e38\u4f7f\u7528\u4e0d\u540c\u989c\u8272\u6765\u8868\u793a\u9ad8\u8868\u8fbe\u548c\u4f4e\u8868\u8fbe\u3002\u70ed\u56fe\u53ef\u4ee5\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u53d1\u73b0\u6837\u672c\u4e4b\u95f4\u7684\u76f8\u4f3c\u6027\u548c\u5dee\u5f02\u6027\uff0c\u8bc6\u522b\u51fa\u5171\u540c\u8868\u8fbe\u7684\u57fa\u56e0\u6a21\u5f0f\uff0c\u4ee5\u53ca\u53d1\u73b0\u4e0e\u7279\u5b9a\u6761\u4ef6\u76f8\u5173\u7684\u57fa\u56e0\u7fa4\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;\">\u9996\u5148\u6211\u4eec\u6839\u636e\u4e0a\u4e00\u5c0f\u8282\u5f97\u5230\u7684\u5dee\u5f02\u5206\u6790\u7ed3\u679c\u6765\u83b7\u53d6\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u540d\u79f0\u6216ID\u3002\u6211\u4eec\u5148\u6839\u636ep\u503c\u5bf9\u5dee\u5f02\u5206\u6790\u7ed3\u679c\u8fdb\u884c\u6392\u5e8f\uff0c\u4f7f\u5dee\u5f02\u8d8a\u5927\u7684\u57fa\u56e0\u6392\u4f4d\u8d8a\u524d\u3002<\/span><span style=\"font-family: \u7b49\u7ebf;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\">diff_res <- diff_res[order(res$pvalue),]<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 10.5pt;\">\u7136\u540e\u6211\u4eec\u5206\u522b\u83b7\u53d6\u4e0a\u8c03\u57fa\u56e0\u4e2d\u6392\u540d\u524d20\u7684\u57fa\u56e0\uff0c\u4e0e\u4e0b\u8c03\u57fa\u56e0\u4e2d\u6392\u540d\u524d20\u7684\u57fa\u56e0\uff0c\u5e76\u5c06\u5176\u5408\u5e76\u8d77\u6765\u3002\u8fd9\u91cc\u53ef\u4ee5\u6839\u636e\u9700\u8981\u8bbe\u7f6e\u6240\u8981\u5c55\u793a\u7684\u57fa\u56e0\u6570\u91cf\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code><span class=\"code-snippet_outer\">res_up_gene <- subset(diff_res,padj <= 0.05 & log2FoldChange >= 2)<\/span><\/code><code><span class=\"code-snippet_outer\">res_down_gene <- subset(diff_res,padj <= 0.05 & log2FoldChange <= -2)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">res_up_gene_top20 <- as.vector(rownames(head(res_up_gene,20)))<\/span><\/code><code><span class=\"code-snippet_outer\">res_down_gene_top20 <- as.vector(rownames(head(res_down_gene,20)))<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">res_top40 <- c(res_up_gene_top20,res_down_gene_top20)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u6211\u4eec\u83b7\u53d6\u5230\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u901a\u8fc7\u70ed\u56fe\u66f4\u52a0\u76f4\u89c2\u5730\u6765\u5448\u73b0\u4e24\u7ec4\u6837\u672c\u4e4b\u95f4\u57fa\u56e0\u8868\u8fbe\u7684\u5dee\u5f02\u3002\u9996\u5148\u5bfc\u5165\u7ed8\u5236\u70ed\u56fe\u6240\u9700\u8981\u7528\u5230\u7684R\u5305\u201cpheatmap\u201d\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u8fd9\u91cc\u6211\u4eec\u9700\u8981\u4f7f\u7528\u4e0a\u4e00\u5c0f\u8282DESeq2\u5305\u4e2d\u6211\u4eec\u6784\u5efa\u7684dds\u5bf9\u8c61\uff0c\u83b7\u53d6dds\u5bf9\u8c61\u4e2d\u6807\u51c6\u5316\u540e\u7684counts \u77e9\u9635\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\">normalized_counts <span class=\"code-snippet__tag\"><<span class=\"code-snippet__name\">-<\/span> <span class=\"code-snippet__attr\">counts<\/span>(<span class=\"code-snippet__attr\">dds<\/span>,<span class=\"code-snippet__attr\">normalized<\/span>=<span class=\"code-snippet__string\">TRUE)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u8fd9\u91cc\u6211\u4eec\u9009\u62e9\u524d40\u4e2a\u5dee\u5f02\u57fa\u56e0\u8fdb\u884c\u5c55\u793a\u3002<\/span><\/p>\n<p><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\">normalized_counts <span class=\"code-snippet__tag\"><<span class=\"code-snippet__name\">-<\/span> <span class=\"code-snippet__attr\">counts<\/span>(<span class=\"code-snippet__attr\">dds<\/span>,<span class=\"code-snippet__attr\">normalized<\/span>=<span class=\"code-snippet__string\">TRUE)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u7136\u540e\u76f4\u63a5\u8c03\u7528pheatmap\u7ed8\u5236\u70ed\u56fe\uff0ccluster_cols\u662f\u8bbe\u7f6e\u662f\u5426\u8fdb\u884c\u5217\u805a\u7c7b\uff0ccluster_rows\u662f\u662f\u5426\u8fdb\u884c\u884c\u805a\u7c7b\uff0cscale\u4ee3\u8868\u7740\u6570\u503c\u662f\u5426\u8fdb\u884c\u6807\u51c6\u5316\uff0c\u53ef\u4ee5\u89c4\u5b9a\u6309\u7167\u884c\u6216\u8005\u6309\u7167\u5217\u6765\u8fdb\u884c\u5f52\u4e00\u5316\u3002annotation_col\u662f\u5217\u7684\u5206\u7ec4\u4fe1\u606f\uff0c\u5bf9\u5e94\u5730pheatmap\u5305\u4e5f\u5177\u6709\u8bbe\u7f6e\u884c\u5206\u7ec4\u4fe1\u606f\u7684\u53c2\u6570annotation_row\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">pheatmap(dat,<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">cluster_cols<\/span> =<span class=\"code-snippet__string\">T, <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">cluster_rows<\/span> = <span class=\"code-snippet__string\">T,<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">scale<\/span>=<span class=\"code-snippet__string\">\"row\", #\u6821\u6b63\uff0c\u89c6\u56fe\u5f62\u800c\u5b9a<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">annotation_col<\/span> = <span class=\"code-snippet__string\">coldata<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">dev.off()<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7417027417027418\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_DmSJos5A6ar0T05w8Dn7EfxzBSicMA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxkQdNGeWtDGfzAUzolwgtQO75fOFCYOvxcibtGYNzAqR5aicUDmSJos5A6ar0T05w8Dn7EfxzBSicMA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, 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.code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar 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id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-08-22 19:00:12<\/em><\/div>\n<p><content><\/p>\n<section style=\"font-size: 16px;\" data-mpa-powered-by=\"yiban.io\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: left;\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section style=\"letter-spacing: 0.578px;\">\n<section>\n<section style=\"width: 100%;flex: 0 0 100%;\" powered-by=\"xiumi.us\">\n<section style=\"opacity: 1;width: 100%;\">\n<section powered-by=\"xiumi.us\">\n<section style=\"line-height: 1.8;\">\n<section style=\"margin-top: 10px;margin-bottom: 20px;width: 100%;\" powered-by=\"xiumi.us\">\n<section style=\"flex-flow: row nowrap;text-align: center;width: 100%;\">\n<section style=\"display: flex;justify-content: center;flex-direction: row;\">\n<section style=\"display: inline-block;width: 100%;flex: 0 0 auto;align-self: flex-start;vertical-align: top;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-style: solid;border-width: 1px;border-color: rgb(67, 67, 67);height: auto;box-shadow: rgb(255, 219, 118) 6px 6px 0px;background-color: rgb(255, 255, 255);width: 100%;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;vertical-align: top;width: 70.0348%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 70.0348% !important;\">\n<section style=\"margin-top: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 29.9652%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 29.9652% !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;line-height: 0;\">\n<section>\n<section style=\"display: inline-block;vertical-align: middle;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 0;\">\n<section style=\"width: 111px;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 1.75em;text-indent: 0em;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;height: auto;width: 111px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_GBhsibRAgNiaQsH4ichdP9ibEhHMFA-89.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYngnlT6jiavu08Bvp2iblzGf3IkoBD9NGBhsibRAgNiaQsH4ichdP9ibEhHMFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"400\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section style=\"display: contents;\" powered-by=\"xiumi.us\">\n<section style=\"display: contents;\">\n<section style=\"display: contents;\">\n<section style=\"height: 0px;overflow: hidden;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;caret-color: red;\"> <\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<p><span style=\"font-size: 14px;\">\u4e0a\u4e00\u8282\uff0c\u5c0f\u679c\u5411\u5927\u5bb6\u4ecb\u7ecd\u4e86DESeq2\u5dee\u5f02\u5206\u6790\u7684\u65b9\u6cd5\uff0cDESeq2\u5305\u4e5f\u5185\u7f6e\u4e00\u4e9b\u7b80\u5355\u7684\u53ef\u89c6\u5316\u51fd\u6570\uff0c\u53ea\u80fd\u6ee1\u8db3\u6211\u4eec\u7684\u4e00\u4e9b\u57fa\u7840\u9700\u6c42\uff0c\u5982\u4f55\u5c06\u7ed3\u679c\u66f4\u4e30\u5bcc\u7f8e\u89c2\u5730\u8fdb\u884c\u53ef\u89c6\u5316\u5448\u73b0\u5462\uff1f\u706b\u5c71\u56fe\uff08Volcano plot\uff09\u548c\u70ed\u56fe\uff08Heatmap\uff09\u662f\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u4e2d\u5e38\u7528\u7684\u6570\u636e\u53ef\u89c6\u5316\u5de5\u5177\uff0c\u5b83\u4eec\u5728\u7ed3\u679c\u89e3\u91ca\u548c\u6570\u636e\u63a2\u7d22\u4e2d\u8d77\u7740\u91cd\u8981\u7684\u4f5c\u7528\u3002\u4e0b\u9762\u63a5\u7740\u8ddf\u5c0f\u679c\u4e00\u8d77\u6765\u5b66\u4e60\u5427\uff01<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 20px;\"><em><strong>1.\u706b\u5c71\u56fe<\/strong><\/em><\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u706b\u5c71\u56fe\u662f\u4e00\u79cd\u6563\u70b9\u56fe\uff0c\u7528\u4e8e\u53ef\u89c6\u5316\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u7edf\u8ba1\u663e\u8457\u6027\u548c\u751f\u7269\u5b66\u91cd\u8981\u6027\u3002\u5728\u706b\u5c71\u56fe\u4e2d\uff0c\u6a2a\u8f74\u8868\u793a\u57fa\u56e0\u7684\u5dee\u5f02\u8868\u8fbe\u6c34\u5e73\uff08\u5982\u6298\u53e0\u53d8\u5316\u6216\u5bf9\u6570\u53d8\u5316\uff09\uff0c\u7eb5\u8f74\u8868\u793a\u57fa\u56e0\u7684\u7edf\u8ba1\u663e\u8457\u6027\uff08\u5982\u8c03\u6574\u7684 p-value\uff09\u3002\u6bcf\u4e2a\u57fa\u56e0\u4ee5\u4e00\u4e2a\u70b9\u7684\u5f62\u5f0f\u8868\u793a\uff0c\u70b9\u7684\u4f4d\u7f6e\u53d6\u51b3\u4e8e\u57fa\u56e0\u7684\u5dee\u5f02\u8868\u8fbe\u7a0b\u5ea6\u548c\u7edf\u8ba1\u663e\u8457\u6027\u3002\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u901a\u5e38\u4f4d\u4e8e\u56fe\u50cf\u7684\u9876\u90e8\u6216\u5e95\u90e8\uff0c\u800c\u975e\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u5219\u4f4d\u4e8e\u56fe\u50cf\u7684\u4e2d\u592e\u3002\u901a\u8fc7\u89c2\u5bdf\u706b\u5c71\u56fe\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u5feb\u901f\u8bc6\u522b\u51fa\u5dee\u5f02\u8868\u8fbe\u6c34\u5e73\u663e\u8457\u7684\u57fa\u56e0\uff0c\u5e76\u4e86\u89e3\u5b83\u4eec\u7684\u751f\u7269\u5b66\u91cd\u8981\u6027\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u9996\u5148\u6211\u4eec\u9700\u8981\u73b0\u8bbe\u7f6e\u7b5b\u9009\u9608\u503c\uff0c\u5bf9\u4e8e\u5dee\u5f02\u5206\u6790\u7684\u7ed3\u679c\u6765\u8bf4\uff0c\u6709\u4e24\u4e2a\u6bd4\u8f83\u91cd\u8981\u7684\u9608\u503c\uff0c\u5206\u522b\u662f\u77eb\u6b63\u540e\u7684P\u503c\u548clog2FoldChange\u503c\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u77eb\u6b63\u540e\u7684P\u503c\u5c5e\u4e8e\u7edf\u8ba1\u503c\u9608\u503c\uff0c\u5728\u5dee\u5f02\u5206\u6790\u7684\u8fc7\u7a0b\u4e2d\u4f1a\u91c7\u7528\u516c\u8ba4\u7684Benjamini-Hochberg\u6821\u6b63\u65b9\u6cd5\u5bf9\u539f\u6709\u5047\u8bbe\u68c0\u9a8c\u5f97\u5230\u7684\u663e\u8457\u6027p\u503c\u8fdb\u884c\u6821\u6b63\uff0c\u4e00\u822c\u9009\u62e90.01\u6216\u80050.05\u4f5c\u4e3a\u6807\u51c6\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">log2FoldChange\u503c\u662f\u8868\u8fbe\u91cf\u7684\u9608\u503c\uff0c\u8fd9\u4e2a\u503c\u4ee3\u8868\u4e24\u7ec4\u6837\u672c\u95f4\u8868\u8fbe\u91cf\u7684\u6bd4\u503c\uff0c\u4e00\u822c\u5c06\u5dee\u5f02\u57fa\u56e0\u7684\u7b5b\u9009\u6807\u51c6\u8bbe\u7f6e\u4e3alog2FoldChange\u7edd\u5bf9\u503c\u5927\u4e8e1\u6216\u80052\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u8fd9\u4e24\u4e2a\u6307\u6807\u7684\u9009\u53d6\u90fd\u662f\u6839\u636e\u7ecf\u9a8c\u53bb\u8fdb\u884c\u8bbe\u7f6e\uff0c\u5e76\u4e0d\u662f\u5b8c\u5168\u56fa\u5b9a\uff0c\u5728\u5177\u4f53\u5206\u6790\u8fc7\u7a0b\u4e2d\uff0c\u6211\u4eec\u901a\u5e38\u9700\u8981\u6839\u636e\u5f97\u5230\u7684\u5dee\u5f02\u57fa\u56e0\u6570\u76ee\u8fdb\u884c\u5fae\u8c03\u3002<\/span><o:p><\/o:p><\/p>\n<p><span style=\"font-size: 14px;\">\u4ee3\u7801\u5982\u4e0b\uff1a<\/span><\/p>\n<section powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">##\u8bbe\u7f6e\u7b5b\u9009\u9608\u503c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">logFoldChange = 2<\/span><\/code><code><span class=\"code-snippet_outer\">adjustP = 0.05<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">diff_res <- as.data.frame(res)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">diff_res[is.na(diff_res)] <- 0<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u5728\u706b\u5c71\u56fe\u4e2d\u6211\u4eec\u53ef\u4ee5\u770b\u5230\uff0c\u7070\u8272\u7684\u7ebf\u5c31\u4ee3\u8868\u7740\u8fd9\u4e24\u4e2a\u6307\u6807\u7684\u9608\u503c\u3002\u8fd9\u91cc\u6211\u4eec\u5c06P\u503c\u5c0f\u4e8e0.05\u5e76\u4e14log2FoldChange\u503c\u5927\u4e8e2\u7684\u57fa\u56e0\u5b9a\u4e49\u4e3a\u4e0a\u8c03\u57fa\u56e0\uff0c\u5c06P\u503c\u5c0f\u4e8e0.05\u5e76\u4e14log2FoldChange\u503c\u5c0f\u4e8e-2\u7684\u57fa\u56e0\u5b9a\u4e49\u4e3a\u4e0b\u8c03\u57fa\u56e0\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"white-space:pre-wrap;font-family: Consolas, "Liberation Mono", Menlo, Courier, monospace;font-size: 14px;background-color: rgb(250, 250, 250);color: rgb(51, 51, 51);letter-spacing: 0.034em;\">\u200d<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\">diff_res$change <- ifelse(diff_res$padj > adjustP,'stable',<\/span><\/code><code><span class=\"code-snippet_outer\"> ifelse(diff_res$log2FoldChange > logFoldChange,'UP',<\/span><\/code><code><span class=\"code-snippet_outer\"> ifelse(diff_res$log2FoldChange < -logFoldChange,'DOWN','stable'))<\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\u6211\u4eec\u5bfc\u5165\u7ed8\u56fe\u6240\u7528R\u5305ggplot2\uff0c\u8bbe\u7f6e\u56fe\u7247\u540d\u79f0\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code><span class=\"code-snippet_outer\">#\u753b\u706b\u5c71\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"># \u5bfc\u5165\u7ed8\u56fe\u5305<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">title <- paste(\"DEGs Volcano Plot\", sep = \"\", collapse = \"\")<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u5bf9\u5e94\u8bbe\u7f6e\u7ed3\u679c\u6570\u636e\uff0cx\u8f74\u4e0ey\u8f74\u6240\u7ed8\u5236\u7684\u6570\u636e\uff0c\u6839\u636e\u4e0a\u4e00\u6b65\u6240\u5b9a\u4e49\u7684\u4e0a\u4e0b\u8c03\u57fa\u56e0\u6765\u7ed8\u5236\u70b9\u7684\u989c\u8272\u3002geom_hline\u4e0egeom_vline\u51fd\u6570\u7528\u4e8e\u7ed8\u5236\u6c34\u5e73\u4e0e\u5782\u76f4\u7684\u8f85\u52a9\u7ebf\u3002<span style=\"font-family: \u7b49\u7ebf;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/span><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code><span class=\"code-snippet_outer\">ggplot(data=diff_res, aes(x=log2FoldChange, y=-log10(padj), color=change)) + <\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(alpha=0.4, size=1.75) +<\/span><\/code><code><span class=\"code-snippet_outer\"> theme_set(theme_set(theme_bw(base_size=20))) +<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab(\"log2 fold change\") + ylab(\"-log10 adj.P.Val\") +<\/span><\/code><code><span class=\"code-snippet_outer\"> ggtitle(title) + theme(plot.title = element_text(size=15,hjust = 0.5)) +<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_colour_manual(values = c('#FF9999','gray80','#99CC00')) + #\u81ea\u5b9a\u4e49\u989c\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_hline(yintercept = c(-log10(adjustP)),linewidth = 1, color = \"grey\", lty = \"dashed\") +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_vline(xintercept = c(-2,2),linewidth = 1, color = \"grey\", lty = \"dashed\") # \u9608\u503c\u8f85\u52a9\u7ebf<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7489177489177489\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_T338otxFib1Oo0xuIHRgDFLVuTjguQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxkQdNGeWtDGfzAUzolwgtQOJzLia4RDYwxFOer5avr3DMrboT338otxFib1Oo0xuIHRgDFLVuTjguQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;font-size: 16px;\"><br \/><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 20px;\"><em><strong>2.\u70ed\u56fe<\/strong><\/em><\/span><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u70ed\u56fe\u662f\u4e00\u79cd\u77e9\u9635\u5f0f\u7684\u56fe\u5f62\u8868\u793a\u65b9\u6cd5\uff0c\u7528\u4e8e\u53ef\u89c6\u5316\u57fa\u56e0\u7684\u8868\u8fbe\u6a21\u5f0f\u3002\u5728\u70ed\u56fe\u4e2d\uff0c\u6bcf\u884c\u4ee3\u8868\u4e00\u4e2a\u57fa\u56e0\uff0c\u6bcf\u5217\u4ee3\u8868\u4e00\u4e2a\u6837\u672c\u3002\u901a\u8fc7\u5bf9\u57fa\u56e0\u8868\u8fbe\u91cf\u8fdb\u884c\u5f52\u4e00\u5316\u548c\u805a\u7c7b\uff0c\u70ed\u56fe\u5c55\u793a\u4e86\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u7684\u76f8\u4f3c\u6027\u548c\u5dee\u5f02\u6027\u3002\u70ed\u56fe\u4e2d\u7684\u989c\u8272\u7f16\u7801\u8868\u793a\u57fa\u56e0\u8868\u8fbe\u91cf\u7684\u9ad8\u4f4e\uff0c\u901a\u5e38\u4f7f\u7528\u4e0d\u540c\u989c\u8272\u6765\u8868\u793a\u9ad8\u8868\u8fbe\u548c\u4f4e\u8868\u8fbe\u3002\u70ed\u56fe\u53ef\u4ee5\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u53d1\u73b0\u6837\u672c\u4e4b\u95f4\u7684\u76f8\u4f3c\u6027\u548c\u5dee\u5f02\u6027\uff0c\u8bc6\u522b\u51fa\u5171\u540c\u8868\u8fbe\u7684\u57fa\u56e0\u6a21\u5f0f\uff0c\u4ee5\u53ca\u53d1\u73b0\u4e0e\u7279\u5b9a\u6761\u4ef6\u76f8\u5173\u7684\u57fa\u56e0\u7fa4\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;\">\u9996\u5148\u6211\u4eec\u6839\u636e\u4e0a\u4e00\u5c0f\u8282\u5f97\u5230\u7684\u5dee\u5f02\u5206\u6790\u7ed3\u679c\u6765\u83b7\u53d6\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u540d\u79f0\u6216ID\u3002\u6211\u4eec\u5148\u6839\u636ep\u503c\u5bf9\u5dee\u5f02\u5206\u6790\u7ed3\u679c\u8fdb\u884c\u6392\u5e8f\uff0c\u4f7f\u5dee\u5f02\u8d8a\u5927\u7684\u57fa\u56e0\u6392\u4f4d\u8d8a\u524d\u3002<\/span><span style=\"font-family: \u7b49\u7ebf;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\">diff_res <- diff_res[order(res$pvalue),]<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 10.5pt;\">\u7136\u540e\u6211\u4eec\u5206\u522b\u83b7\u53d6\u4e0a\u8c03\u57fa\u56e0\u4e2d\u6392\u540d\u524d20\u7684\u57fa\u56e0\uff0c\u4e0e\u4e0b\u8c03\u57fa\u56e0\u4e2d\u6392\u540d\u524d20\u7684\u57fa\u56e0\uff0c\u5e76\u5c06\u5176\u5408\u5e76\u8d77\u6765\u3002\u8fd9\u91cc\u53ef\u4ee5\u6839\u636e\u9700\u8981\u8bbe\u7f6e\u6240\u8981\u5c55\u793a\u7684\u57fa\u56e0\u6570\u91cf\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code><span class=\"code-snippet_outer\">res_up_gene <- subset(diff_res,padj <= 0.05 & log2FoldChange >= 2)<\/span><\/code><code><span class=\"code-snippet_outer\">res_down_gene <- subset(diff_res,padj <= 0.05 & log2FoldChange <= -2)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">res_up_gene_top20 <- as.vector(rownames(head(res_up_gene,20)))<\/span><\/code><code><span class=\"code-snippet_outer\">res_down_gene_top20 <- as.vector(rownames(head(res_down_gene,20)))<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">res_top40 <- c(res_up_gene_top20,res_down_gene_top20)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u6211\u4eec\u83b7\u53d6\u5230\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u901a\u8fc7\u70ed\u56fe\u66f4\u52a0\u76f4\u89c2\u5730\u6765\u5448\u73b0\u4e24\u7ec4\u6837\u672c\u4e4b\u95f4\u57fa\u56e0\u8868\u8fbe\u7684\u5dee\u5f02\u3002\u9996\u5148\u5bfc\u5165\u7ed8\u5236\u70ed\u56fe\u6240\u9700\u8981\u7528\u5230\u7684R\u5305\u201cpheatmap\u201d\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u8fd9\u91cc\u6211\u4eec\u9700\u8981\u4f7f\u7528\u4e0a\u4e00\u5c0f\u8282DESeq2\u5305\u4e2d\u6211\u4eec\u6784\u5efa\u7684dds\u5bf9\u8c61\uff0c\u83b7\u53d6dds\u5bf9\u8c61\u4e2d\u6807\u51c6\u5316\u540e\u7684counts \u77e9\u9635\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\">normalized_counts <span class=\"code-snippet__tag\"><<span class=\"code-snippet__name\">-<\/span> <span class=\"code-snippet__attr\">counts<\/span>(<span class=\"code-snippet__attr\">dds<\/span>,<span class=\"code-snippet__attr\">normalized<\/span>=<span class=\"code-snippet__string\">TRUE)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u8fd9\u91cc\u6211\u4eec\u9009\u62e9\u524d40\u4e2a\u5dee\u5f02\u57fa\u56e0\u8fdb\u884c\u5c55\u793a\u3002<\/span><\/p>\n<p><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\">normalized_counts <span class=\"code-snippet__tag\"><<span class=\"code-snippet__name\">-<\/span> <span class=\"code-snippet__attr\">counts<\/span>(<span class=\"code-snippet__attr\">dds<\/span>,<span class=\"code-snippet__attr\">normalized<\/span>=<span class=\"code-snippet__string\">TRUE)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u7136\u540e\u76f4\u63a5\u8c03\u7528pheatmap\u7ed8\u5236\u70ed\u56fe\uff0ccluster_cols\u662f\u8bbe\u7f6e\u662f\u5426\u8fdb\u884c\u5217\u805a\u7c7b\uff0ccluster_rows\u662f\u662f\u5426\u8fdb\u884c\u884c\u805a\u7c7b\uff0cscale\u4ee3\u8868\u7740\u6570\u503c\u662f\u5426\u8fdb\u884c\u6807\u51c6\u5316\uff0c\u53ef\u4ee5\u89c4\u5b9a\u6309\u7167\u884c\u6216\u8005\u6309\u7167\u5217\u6765\u8fdb\u884c\u5f52\u4e00\u5316\u3002annotation_col\u662f\u5217\u7684\u5206\u7ec4\u4fe1\u606f\uff0c\u5bf9\u5e94\u5730pheatmap\u5305\u4e5f\u5177\u6709\u8bbe\u7f6e\u884c\u5206\u7ec4\u4fe1\u606f\u7684\u53c2\u6570annotation_row\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:\u7b49\u7ebf;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">pheatmap(dat,<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">cluster_cols<\/span> =<span class=\"code-snippet__string\">T, <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">cluster_rows<\/span> = <span class=\"code-snippet__string\">T,<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">scale<\/span>=<span class=\"code-snippet__string\">\"row\", #\u6821\u6b63\uff0c\u89c6\u56fe\u5f62\u800c\u5b9a<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">annotation_col<\/span> = <span class=\"code-snippet__string\">coldata<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__attr\">)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">dev.off()<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.7417027417027418\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_DmSJos5A6ar0T05w8Dn7EfxzBSicMA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxkQdNGeWtDGfzAUzolwgtQO75fOFCYOvxcibtGYNzAqR5aicUDmSJos5A6ar0T05w8Dn7EfxzBSicMA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, 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"Microsoft YaHei", Arial, sans-serif;\"><\/span><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 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[…]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/56981"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=56981"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/56981\/revisions"}],"predecessor-version":[{"id":56985,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/56981\/revisions\/56985"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=56981"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=56981"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=56981"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}