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"Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">\u3002bioawk\u53ef\u4ee5\u8ba9\u4f60\u5feb\u901f\u5730\u5bf9\u8fd9\u4e9b\u6570\u636e\u8fdb\u884c\u8fc7\u6ee4\uff0c\u8f6c\u6362\uff0c\u7edf\u8ba1\u548c\u5206\u6790\uff0c\u800c\u4e0d\u9700\u8981\u5199\u590d\u6742\u7684\u811a\u672c\u6216\u8005\u4f7f\u7528\u5176\u4ed6\u7684\u8f6f\u4ef6\u3002bioawk\u7684\u8bed\u6cd5\u548cawk\u57fa\u672c\u4e00\u81f4\uff0c\u4f46\u662f\u589e\u52a0\u4e86\u4e00\u4e9b\u9488\u5bf9\u751f\u7269\u4fe1\u606f\u5b66\u6570\u636e\u7684\u7279\u6027\uff0c\u6bd4\u5982\u53ef\u4ee5\u76f4\u63a5\u8bfb\u53d6\u5e8f\u5217\u7684\u957f\u5ea6\uff0c\u8d28\u91cf\uff0c\u6ce8\u91ca\u7b49\u4fe1\u606f\uff0c\u4e5f\u53ef\u4ee5\u65b9\u4fbf\u5730\u5bf9\u5e8f\u5217\u8fdb\u884c\u53cd\u5411\u4e92\u8865\uff0c\u7ffb\u8bd1\u7b49\u64cd\u4f5c\u3002bioawk\u8fd8\u652f\u6301\u591a\u79cd\u8f93\u51fa\u683c\u5f0f\uff0c\u6bd4\u5982CSV\uff0cTSV\uff0cJSON\u7b49\uff0c\u65b9\u4fbf\u4f60\u5c06\u7ed3\u679c\u5bfc\u5165\u5176\u4ed6\u7684\u7a0b\u5e8f\u6216\u8005\u5e73\u53f0\u8fdb\u884c\u8fdb\u4e00\u6b65\u7684\u5206\u6790\u3002<\/span><\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">\u63a5\u4e0b\u6765\uff0c\u5c0f\u679c\u5c06\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e9bbioawk\u7684\u57fa\u672c\u7528\u6cd5\u548c\u5e38\u89c1\u7684\u5e94\u7528\u573a\u666f\u3002\u5982\u679c\u4f60\u5bf9bioawk\u611f\u5174\u8da3\uff0c\u6216\u8005\u60f3\u8981\u63d0\u9ad8\u4f60\u7684\u6587\u672c\u5904\u7406\u80fd\u529b\uff0c\u90a3\u4e48\u8bf7\u7ee7\u7eed\u9605\u8bfb\u5427\u3002<\/span>    <\/span><o:page><\/o:page><\/section>\n<h2 style=\"text-indent: 0em;text-align: justify;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">bioawk\u7684\u5b89\u88c5<\/span><\/strong><\/span><\/h2>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">bioawk\u662f\u4e00\u4e2a\u5f00\u6e90\u7684\u8f6f\u4ef6\uff0c\u4f60\u53ef\u4ee5\u4eceGitHub\u4e0a\u514b\u9686\u5b83\u7684\u6e90\u4ee3\u7801\uff0c\u5e76\u4e14\u6309\u7167\u8bf4\u660e\u8fdb\u884c\u7f16\u8bd1\u548c\u5b89\u88c5\u3002\u4f60\u4e5f\u53ef\u4ee5\u4f7f\u7528conda\u6216\u8005brew\u7b49\u5305\u7ba1\u7406\u5668\u6765\u5b89\u88c5bioawk\u3002<\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);font-size: 15px;\">\u6e90\u4ee3\u7801\u4e0b\u8f7d\u7f51\u5740\uff1a<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);font-size: 15px;\">https:\/\/github.com\/lh3\/bioawk<\/span><\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;background: rgb(255, 255, 255);font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">\u5b89\u88c5\u547d\u4ee4\uff1a<\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100012476\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_IWicymEetCc6Enf2BkUE4Yz1Ya5e5w-9.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyuYsqlJnQUJvnS2iciaDibtfbdV0VmHlriaSjYwjFPLp1BLjokHIWicymEetCc6Enf2BkUE4Yz1Ya5e5w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"color: rgb(0, 0, 0);font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.578px;text-wrap: wrap;text-align: center;\" data-recalc-dims=\"1\" \/><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">$<\/span> git <span class=\"code-snippet__built_in\">clone<\/span> git:\/\/github.com\/lh3\/bioawk.git <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">$<\/span> <span class=\"code-snippet__built_in\">cd<\/span> bioaw<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">$<\/span> make<\/span><\/code><code><span class=\"code-snippet_outer\">\u6216\u8005connda\u5b89\u88c5\uff1a<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">$<\/span>\uff1aconnda install -p ~\/YOUR\/conda path\/ bioawk -y<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-left: 0pt;line-height: normal;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;background: rgb(255, 255, 255);font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.5787037037037037\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_wESPQXS9jQh3tpNicVb6FeQYUwK93g-1.png?resize=514%2C297\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOK87rsWVHeosLicpooZLLX5TVzywESPQXS9jQh3tpNicVb6FeQYUwK93g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"297 .7099609375\" width=\"514 .4099731445312\" data-recalc-dims=\"1\" \/><\/span><\/section>\n<h2 style=\"text-indent: 0em;text-align: justify;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">bioawk\u7684\u57fa\u672c\u8bed\u6cd5<\/span><br \/>\n  <\/span><\/h2>\n<section style=\"margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: justify;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;line-height: 150%;font-variant: normal;text-transform: none;color: rgb(0, 0, 0);\">Bioawk\u662f\u4e00\u4e2a\u547d\u4ee4\u884c\u5de5\u5177\uff0c\u5b83\u7684\u7528\u6cd5\u662f\uff1a.\/bioawk [-F fs] [-v var=value] [-c fmt] [-tH] [-f progfile | ‘prog’] [file …]\u3002\u4e0b\u9762\u662f\u5404\u4e2a\u9009\u9879\u7684\u89e3\u91ca\uff1a<\/span><\/section>\n<section style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: justify;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;line-height: 150%;font-variant: normal;text-transform: none;\">\u00b7-F fs\uff1a\u6307\u5b9a\u8f93\u5165\u5b57\u6bb5\u5206\u9694\u7b26\u3002\u9ed8\u8ba4\u4e3a\u201c\u7a7a\u683c\u201d\u3002<\/span>    <\/span><o:page><\/o:page><\/section>\n<section style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: justify;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;line-height: 150%;font-variant: normal;text-transform: none;color: rgb(0, 0, 0);\">\u00b7-v var=value\uff1a\u5728\u7a0b\u5e8f\u5f00\u59cb\u8fd0\u884c\u4e4b\u524d\uff0c\u5c06\u53d8\u91cfvar\u8bbe\u7f6e\u4e3a\u503cvalue\u3002<\/span><\/section>\n<section style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: justify;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;line-height: 150%;font-variant: normal;text-transform: none;color: rgb(0, 0, 0);\">\u00b7-c fmt\uff1a\u6307\u5b9a\u8f93\u5165\u683c\u5f0f\u3002\u5f53\u4f7f\u7528\u6b64\u9009\u9879\u65f6\uff0cbioawk\u5c06\u6839\u636e\u683c\u5f0f\u6216\u8f93\u5165\u7684\u7b2c\u4e00\u884c\u65e0\u7f1d\u6dfb\u52a0\u547d\u540d\u5b57\u6bb5\u7684\u53d8\u91cf\uff0c\u5177\u4f53\u53d6\u51b3\u4e8efmt\u3002\u6b64\u9009\u9879\u8fd8\u4f7fbioawk\u80fd\u591f\u8bfb\u53d6gzip\u538b\u7f29\u7684\u6587\u4ef6\u3002<\/span><\/section>\n<section style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: justify;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;line-height: 150%;font-variant: normal;text-transform: none;color: rgb(0, 0, 0);\">\u00b7-t\uff1a\u4f7f\u7528\u6b64\u9009\u9879\u7b49\u540c\u4e8ebioawk -F\u2019t\u2019 -v OFS=”t”\u3002<\/span><\/section>\n<section style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: justify;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;line-height: 150%;font-variant: normal;text-transform: none;color: rgb(0, 0, 0);\">\u00b7-H\uff1a\u6253\u5370\u8f93\u51fa\u6807\u9898\u884c\u3002<\/span><\/section>\n<section style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: justify;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;line-height: 150%;font-variant: normal;text-transform: none;color: rgb(0, 0, 0);\">\u00b7-f progfile\uff1a\u4ece\u6587\u4ef6progfile\u4e2d\u8bfb\u53d6\u7a0b\u5e8f\u3002<\/span><\/section>\n<section style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: justify;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;line-height: 150%;font-variant: normal;text-transform: none;color: rgb(0, 0, 0);\">\u00b7’prog’\uff1a\u76f4\u63a5\u5728\u547d\u4ee4\u884c\u4e2d\u6307\u5b9a\u7a0b\u5e8f\u3002<\/span><\/section>\n<section style=\"font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-align: justify;margin-left: 36pt;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;line-height: 150%;font-variant: normal;text-transform: none;color: rgb(0, 0, 0);\">\u00b7[file …]\uff1a\u6307\u5b9a\u8981\u5904\u7406\u7684\u6587\u4ef6\u3002\u5982\u679c\u672a\u6307\u5b9a\uff0c\u5219\u4ece\u6807\u51c6\u8f93\u5165\u8bfb\u53d6\u3002<\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">\u6211\u4eec\u53ef\u4ee5\u7528bioawk -c -h\u547d\u4ee4\u5217\u51fa\u652f\u6301\u7684\u683c\u5f0f\uff1a<\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">bed<\/span>:<\/span><\/code><code><span class=\"code-snippet_outer\"> 1<span class=\"code-snippet__selector-pseudo\">:chrom<\/span> 2<span class=\"code-snippet__selector-pseudo\">:start<\/span> 3<span class=\"code-snippet__selector-pseudo\">:end<\/span> 4<span class=\"code-snippet__selector-pseudo\">:name<\/span> 5<span class=\"code-snippet__selector-pseudo\">:score<\/span> 6<span class=\"code-snippet__selector-pseudo\">:strand<\/span> 7<span class=\"code-snippet__selector-pseudo\">:thickstart<\/span> 8<span class=\"code-snippet__selector-pseudo\">:thickend<\/span> 9<span class=\"code-snippet__selector-pseudo\">:rgb<\/span> 10<span class=\"code-snippet__selector-pseudo\">:blockcount<\/span> 11<span class=\"code-snippet__selector-pseudo\">:blocksizes<\/span> 12<span class=\"code-snippet__selector-pseudo\">:blockstarts<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">sam<\/span>:<\/span><\/code><code><span class=\"code-snippet_outer\"> 1<span class=\"code-snippet__selector-pseudo\">:qname<\/span> 2<span class=\"code-snippet__selector-pseudo\">:flag<\/span> 3<span class=\"code-snippet__selector-pseudo\">:rname<\/span> 4<span class=\"code-snippet__selector-pseudo\">:pos<\/span> 5<span class=\"code-snippet__selector-pseudo\">:mapq<\/span> 6<span class=\"code-snippet__selector-pseudo\">:cigar<\/span> 7<span class=\"code-snippet__selector-pseudo\">:rnext<\/span> 8<span class=\"code-snippet__selector-pseudo\">:pnext<\/span> 9<span class=\"code-snippet__selector-pseudo\">:tlen<\/span> 10<span class=\"code-snippet__selector-pseudo\">:seq<\/span> 11<span class=\"code-snippet__selector-pseudo\">:qual<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">vcf<\/span>:<\/span><\/code><code><span class=\"code-snippet_outer\"> 1<span class=\"code-snippet__selector-pseudo\">:chrom<\/span> 2<span class=\"code-snippet__selector-pseudo\">:pos<\/span> 3<span class=\"code-snippet__selector-pseudo\">:id<\/span> 4<span class=\"code-snippet__selector-pseudo\">:ref<\/span> 5<span class=\"code-snippet__selector-pseudo\">:alt<\/span> 6<span class=\"code-snippet__selector-pseudo\">:qual<\/span> 7<span class=\"code-snippet__selector-pseudo\">:filter<\/span> 8<span class=\"code-snippet__selector-pseudo\">:info<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">gff<\/span>:<\/span><\/code><code><span class=\"code-snippet_outer\"> 1<span class=\"code-snippet__selector-pseudo\">:seqname<\/span> 2<span class=\"code-snippet__selector-pseudo\">:source<\/span> 3<span class=\"code-snippet__selector-pseudo\">:feature<\/span> 4<span class=\"code-snippet__selector-pseudo\">:start<\/span> 5<span class=\"code-snippet__selector-pseudo\">:end<\/span> 6<span class=\"code-snippet__selector-pseudo\">:score<\/span> 7<span class=\"code-snippet__selector-pseudo\">:filter<\/span> 8<span class=\"code-snippet__selector-pseudo\">:strand<\/span> 9<span class=\"code-snippet__selector-pseudo\">:group<\/span> 10<span class=\"code-snippet__selector-pseudo\">:attribute<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">fastx<\/span>:<\/span><\/code><code><span class=\"code-snippet_outer\"> 1<span class=\"code-snippet__selector-pseudo\">:name<\/span> 2<span class=\"code-snippet__selector-pseudo\">:seq<\/span> 3<span class=\"code-snippet__selector-pseudo\">:qual<\/span> 4<span class=\"code-snippet__selector-pseudo\">:comment<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-left: 0pt;line-height: normal;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><o:p><\/o:p><\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;font-variant-numeric: normal;font-variant-east-asian: normal;\">bioawk\u7684\u57fa\u672c\u8bed\u6cd5\u548cawk\u4e00\u6837\uff0c\u90fd\u662f`bioawk ‘pattern {action}’ file`\u3002pattern\u662f\u4e00\u4e2a\u5339\u914d\u6761\u4ef6\uff0c\u7528\u4e8e\u9009\u62e9\u7b26\u5408\u6761\u4ef6\u7684\u8bb0\u5f55\uff08record\uff09\u3002action\u662f\u4e00\u4e2a\u6267\u884c\u52a8\u4f5c\uff0c\u7528\u4e8e\u5bf9\u9009\u62e9\u7684\u8bb0\u5f55\u8fdb\u884c\u5904\u7406\u3002file\u662f\u4e00\u4e2a\u8f93\u5165\u6587\u4ef6\uff0c\u901a\u5e38\u662f\u4e00\u4e2a\u751f\u7269\u4fe1\u606f\u5b66\u76f8\u5173\u7684\u6570\u636e\u6587\u4ef6\u3002\u5982\u679c\u6ca1\u6709\u6307\u5b9afile\uff0c\u90a3\u4e48bioawk\u4f1a\u4ece\u6807\u51c6\u8f93\u5165\u4e2d\u8bfb\u53d6\u6570\u636e\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;\">    <\/span><\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><o:page><\/o:page><\/section>\n<h2 style=\"text-indent: 0em;text-align: justify;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">bioawk\u7684\u5e38\u89c1\u5e94\u7528<\/span><br \/>\n  <\/span><\/h2>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;background: rgb(255, 255, 255);font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">\u4e0b\u9762\u6211\u5c06\u7ed9\u5927\u5bb6\u5c55\u793a\u4e00\u4e9bbioawk\u5728\u751f\u7269\u4fe1\u606f\u5b66\u4e2d\u7684\u5e38\u89c1\u5e94\u7528\u3002\u8fd9\u4e9b\u53ea\u662f\u4e00\u4e9b\u7b80\u5355\u7684\u4f8b\u5b50\uff0c\u4f60\u53ef\u4ee5\u6839\u636e\u4f60\u81ea\u5df1\u7684\u9700\u6c42\u548c\u60f3\u8c61\u529b\u6765\u53d1\u6325bioawk\u7684\u66f4\u591a\u529f\u80fd\u3002<\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;font-weight: bold;\">1. \u7edf\u8ba1\u6587\u4ef6\u4fe1\u606f<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">\u3002<\/span><\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">\u5f53\u751f\u4fe1\u5c0f\u767d\u8f9b\u8f9b\u82e6\u82e6\u7ec8\u4e8e\u62ff\u5230\u6211\u4eec\u7684\u6d4b\u5e8f\u7ed3\u679c\u65f6\uff0c\u4f1a\u53d1\u73b0\u5f97\u5230\u4e86\u4e00\u5806\u770b\u4e0d\u61c2\u7684\u4e1c\u897f\/(\u3112o\u3112)\/~~\uff0c\u5982\u4f55\u5c06\u8fd9\u4e00\u5806\u8ba9\u4eba\u8111\u58f3\u75db\u7684\u6570\u636e\u8f6c\u6362\u6210\u6211\u4eec\u53ef\u4ee5\u770b\u61c2\u7684\u6570\u636e\u5462\uff1f<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);\">bioawk\u53ef\u4ee5\u5b9e\u73b0fastx\u6570\u636e\u7684\u7edf\u8ba1\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">\u5982\u679c\u6211\u4eec\u6709\u4e00\u4e2a\u540d\u4e3atest.fastq\u7684FASTQ\u6587\u4ef6\uff0c\u5185\u5bb9\u5982\u4e0b\uff1a<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">          <br \/><\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.18703703703703703\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_XSMuhCeJ5TP8Dnd2rM6ic6ZxobV4WA-1.png?resize=553%2C103\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOnLB39nUnW4UlTaAaP4lzdj4cDXSMuhCeJ5TP8Dnd2rM6ic6ZxobV4WA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"103 .67001342773438\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">\u5982\u679c\u6211\u4eec\u60f3\u7edf\u8ba1\u6d4b\u5e8f\u6570\u636e\u7684\u57fa\u672c\u4fe1\u606f\uff0c<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);\">\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528\u4e0b\u9762\u7684\u547d\u4ee4\u6765\u6253\u5370\u51fa\u6bcf\u6761\u5e8f\u5217\u7684\u540d\u79f0\u3001GC\u6bd4\u548c\u957f\u5ea6\uff1a<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">          <br \/><\/span><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">$ bioawk -c fastx '{ print $name, gc($seq),length($seq) }' test.fastq<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);\">\u8f93\u51fa\u7ed3\u679c\u5982\u4e0b\uff1a<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">          <br \/><\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.11854103343465046\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_D7tRBicBssj1o8ln4KMTPE9SX5v0oQ-1.png?resize=568%2C67\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOmw8KQT6T90fooHkxBEC83BpSbD7tRBicBssj1o8ln4KMTPE9SX5v0oQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"658\" height=\"67 .33001708984375\" width=\"568 .010009765625\" data-recalc-dims=\"1\" \/><\/span><\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);\">\u8fd9\u91cc\u6211\u4eec\u4f7f\u7528\u4e86`-c fastx`\u9009\u9879\u6765\u544a\u8bc9bioawk\u6211\u4eec\u7684\u8f93\u5165\u6587\u4ef6\u662f\u4e00\u4e2aFASTA\u6216\u8005FASTQ\u683c\u5f0f\u7684\u6587\u4ef6\u3002\u8fd9\u6837bioawk\u5c31\u4f1a\u81ea\u52a8\u5c06\u6bcf\u6761\u5e8f\u5217\u4f5c\u4e3a\u4e00\u4e2a\u8bb0\u5f55\uff0c\u5e76\u4e14\u63d0\u4f9b\u4e00\u4e9b\u5185\u7f6e\u7684\u53d8\u91cf\u6765\u8bbf\u95ee\u5e8f\u5217\u7684\u4fe1\u606f\uff0c\u6bd4\u5982$name, $seq, $qual\u7b49\u3002\u6211\u4eec\u8fd8\u4f7f\u7528\u4e86length()\u51fd\u6570\u6765\u8ba1\u7b97\u5e8f\u5217\u7684\u957f\u5ea6\uff0c\u5e76\u4e14\u7528print\u8bed\u53e5\u6765\u6253\u5370\u51fa\u7ed3\u679c\u3002<\/span>    <\/span><o:page><\/o:page><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);\">\u6211\u4eec\u8fd8\u53ef\u4ee5\u5bf9gff\u6587\u4ef6\u8fdb\u884c\u4fe1\u606f\u7edf\u8ba1\u3002\u6bd4\u5982\u8bf4\uff0c\u5982\u679c\u6211\u4eec\u60f3\u8981\u8ba1\u7b97\u4e00\u4e2aGFF\u6587\u4ef6\u4e2d\u6bcf\u79cd\u7279\u5f81\uff08feature\uff09\u7684\u4e2a\u6570\u548c\u603b\u957f\u5ea6\u3002\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528\u4e0b\u9762\u7684\u547d\u4ee4\u6765\u5b9e\u73b0\uff1a<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">          <br \/><\/span><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"swift\"><code><span class=\"code-snippet_outer\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">bioawk -c gff '{count[$3]++; len[$3] += $5 - $4 + 1} END {for (f in count) print f, count[f], len[f]}' test.gff<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">\u8f93\u51fa\u7ed3\u679c\uff1a<\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.35436893203883496\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_iaYGUka91Z3nJStxSibcm3KVF7dEfg-1.png?resize=329%2C116\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOJowBOqxwPObudHUO8Z9NDJ2dwWiaYGUka91Z3nJStxSibcm3KVF7dEfg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"412\" height=\"116 .80999755859375\" width=\"329 .6300048828125\" data-recalc-dims=\"1\" \/><\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);\">\u8fd9\u91cc\u6211\u4eec\u4f7f\u7528\u4e86-c gff\u9009\u9879\u6765\u544a\u8bc9bioawk\u6211\u4eec\u7684\u8f93\u5165\u6587\u4ef6\u662f\u4e00\u4e2aGFF\u683c\u5f0f\u7684\u6587\u4ef6\uff08gff3\u6216\u8005gtf\u4e3a\u6587\u4ef6\uff09\u3002\u8fd9\u6837bioawk\u5c31\u4f1a\u81ea\u52a8\u5c06\u6bcf\u6761\u6ce8\u91ca\u4f5c\u4e3a\u4e00\u4e2a\u8bb0\u5f55\uff0c\u5e76\u4e14\u63d0\u4f9b\u4e00\u4e9b\u5185\u7f6e\u7684\u53d8\u91cf\u6765\u8bbf\u95ee\u6ce8\u91ca\u7684\u4fe1\u606f\uff0c\u6bd4\u5982$1, $2, $3\u7b49\u3002\u6211\u4eec\u8fd8\u4f7f\u7528\u4e86\u6570\u7ec4\uff08array\uff09\u6765\u5b58\u50a8\u6bcf\u79cd\u7279\u5f81\u7684\u4e2a\u6570\u548c\u603b\u957f\u5ea6\uff0c\u5e76\u4e14\u7528END\u5757\u6765\u5728\u5904\u7406\u5b8c\u6240\u6709\u8bb0\u5f55\u540e\u6253\u5370\u51fa\u7ed3\u679c\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">          <br \/><\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;font-weight: bold;background: rgb(255, 255, 255);\">2. \u8fc7\u6ee4\u5e8f\u5217<\/span><\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);\">\u6211\u4eec\u5f97\u5230\u7684\u6d4b\u5e8f\u6570\u636e\u662f\u5b58\u5728\u53c2\u5dee\u7684\uff0c\u4e3a\u4f8b\u5f97\u5230\u66f4\u597d\u7684\u7ed3\u679c\uff0c\u6839\u636e\u4e00\u4e9b\u6761\u4ef6\u6765\u8fc7\u6ee4\u6389\u4e00\u4e9b\u4e0d\u9700\u8981\u7684\u5e8f\u5217\u662f\u5f88\u5fc5\u8981\u7684\u3002\u6bd4\u5982\u8bf4\uff0c\u6211\u4eec\u60f3\u8981\u8fc7\u6ee4\u6389\u957f\u5ea6\u5c0f\u4e8e100bp\u6216\u8005\u8d28\u91cf\u4f4e\u4e8e20\u7684\u5e8f\u5217\u3002\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528\u4e0b\u9762\u7684\u547d\u4ee4\u6765\u5b9e\u73b0\uff1a<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">          <br \/><\/span><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">$ bioawk -c fastx 'length($seq) >= 100 && meanqual($qual) >= 35' test.fastq > filtered_test.fastq<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.08425925925925926\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_fNJic9HQ69UY7P35RHgsfxRE4olicg-1.png?resize=595%2C50\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOHacOTQtXl3PRDtRR1ibwMLVBb5nfNJic9HQ69UY7P35RHgsfxRE4olicg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"50 .4000244140625\" width=\"595 .489990234375\" data-recalc-dims=\"1\" \/><\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);\">\u8fd9\u91cc\u6211\u4eec\u4f7f\u7528\u4e86meanqual()\u51fd\u6570\u6765\u8ba1\u7b97\u5e8f\u5217\u7684\u5e73\u5747\u8d28\u91cf\uff0c\u4ec5\u4fdd\u7559\u5e73\u5747\u8d28\u91cf\u4e0d\u4f4e\u4e8e35\u7684\u5e8f\u5217\uff0c\u5e76\u4e14\u7528&&\u7b26\u53f7\u6765\u8868\u793a\u903b\u8f91\u4e0e\uff08and\uff09\u7684\u5173\u7cfb\u3002\u6211\u4eec\u8fd8\u4f7f\u7528\u4e86\u91cd\u5b9a\u5411\u7b26\u53f7\uff08>\uff09\u6765\u5c06\u7ed3\u679c\u8f93\u51fa\u5230\u4e00\u4e2a\u65b0\u7684\u6587\u4ef6\u4e2d\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">          <br \/><\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;font-weight: bold;background: rgb(255, 255, 255);\">3. \u8f6c\u6362\u683c\u5f0f<\/span>    <\/span><o:page><\/o:page><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);\">\u5728\u751f\u4fe1\u5206\u6790\u7684\u8fc7\u7a0b\u4e2d\uff0c\uff0c\u6211\u4eec\u5f88\u591a\u65f6\u5019\u9700\u8981\u5c06\u4e00\u79cd\u683c\u5f0f\u7684\u6587\u4ef6\u8f6c\u6362\u6210\u53e6\u4e00\u79cd\u683c\u5f0f\u7684\u6587\u4ef6\u3002\u6bd4\u5982\u8bf4\uff0c\u6211\u4eec\u60f3\u8981\u5c06\u4e00\u4e2aFASTQ\u6587\u4ef6\u8f6c\u6362\u6210\u4e00\u4e2aCSV\u6587\u4ef6\uff0c\u6bcf\u884c\u5305\u542b\u5e8f\u5217\u7684\u540d\u79f0\uff0c\u957f\u5ea6\u548cGC\u542b\u91cf\u3002\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528\u4e0b\u9762\u7684\u547d\u4ee4\u6765\u5b9e\u73b0\uff1a<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">          <br \/><\/span><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">bioawk -c fastx -v OFS=',' '{print $name, length($seq), gc($seq)}' test.fastq > test.csv<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"color: rgb(0, 0, 0);\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 15px;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);\">\u7ed3\u679c\u5982\u4e0b\uff1a<\/span><span style=\"font-size: 15px;font-variant: normal;text-transform: none;\">          <br \/><\/span><\/span><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.12759170653907495\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_uuTyGxcVWWOXibQdFCpBZflSoXd0Ug-1.png?resize=501%2C64\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOMgN10FsKuLdp3YaTgZnktudNkuuTyGxcVWWOXibQdFCpBZflSoXd0Ug\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"627\" height=\"64 .010009765625\" width=\"501 .6500244140625\" data-recalc-dims=\"1\" \/><\/span><\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;background: rgb(255, 255, 255);font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">\u8fd9\u91cc\u6211\u4eec\u4f7f\u7528\u4e86-v\u9009\u9879\u6765\u8bbe\u7f6e\u8f93\u51fa\u5b57\u6bb5\u5206\u9694\u7b26\uff08OFS\uff09\u4e3a\u9017\u53f7\uff08,\uff09\u3002\u6211\u4eec\u8fd8\u4f7f\u7528\u4e86print\u8bed\u53e5\u6765\u6253\u5370\u51fa\u7ed3\u679c\u3002<\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;background: rgb(255, 255, 255);font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">Bioawk\u65b0\u589e\u52a0\u4e86\u4e00\u4e2a\u529f\u80fd\uff0c\u53ef\u4ee5\u76f4\u63a5\u4ecefasta\/q\u6587\u4ef6\u4e2d\u83b7\u5f97\u5e8f\u5217\u7684\u53cd\u5411\u4e92\u8865\u5e8f\u5217\uff0c\u547d\u4ee4\u5982\u4e0b\uff1a<\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">bioawk -c fastx '{ print \">\"$name ORS revcomp($seq) }' test.fastq > rtest.fasta<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">\u7ed3\u679c\u5982\u4e0b\uff1a<\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.09814814814814815\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_tO346RvmSEPO5iczTN51rXd0GOTkRg-1.png?resize=553%2C54\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSO1I4Lvicxl8Ao7LibO34ia1OvLkUOtO346RvmSEPO5iczTN51rXd0GOTkRg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"54 .27001953125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">\u5728\u6b64\u547d\u4ee4\u4e2d\uff0c'{ print “>”$name ORS revcomp($seq) }’ \u662f\u4e00\u4e2a awk \u811a\u672c\uff0c\u5b83\u7528\u4e8e\u63d0\u53d6\u6bcf\u4e2a\u8bb0\u5f55\u7684\u540d\u79f0\u548c\u5e8f\u5217\uff0c\u5e76\u8ba1\u7b97\u5e8f\u5217\u7684\u4e92\u8865\u5e8f\u5217\u3002$name \u53d8\u91cf\u8868\u793a\u8bb0\u5f55\u7684\u540d\u79f0\uff0c$seq \u53d8\u91cf\u8868\u793a\u5e8f\u5217\u3002revcomp \u51fd\u6570\u7528\u4e8e\u8ba1\u7b97\u5e8f\u5217\u7684\u4e92\u8865\u5e8f\u5217\u3002ORS \u662f\u8f93\u51fa\u8bb0\u5f55\u5206\u9694\u7b26\uff0c\u5b83\u7684\u9ed8\u8ba4\u503c\u662f\u6362\u884c\u7b26\u3002<\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">Bioawk\u4e5f\u53ef\u4ee5\u4eceBAM\u6587\u4ef6\u4e2d\u63d0\u53d6FASTA\u5e8f\u5217\u3002\u8fd8\u53ef\u4ee5\u6dfb\u52a0\u4e00\u4e9b\u7b5b\u9009\u6761\u4ef6\uff0c\u83b7\u5f97\u6211\u4eec\u60f3\u8981\u7684\u9ad8\u8d28\u91cf\u5e8f\u5217\u3002\u6bd4\u5982\u5982\u679c\u60f3\u8981\u5f97\u5230FLAG\u4e3a16\u7684\u5e8f\u5217\u7684\u65b9\u5411\u4e92\u8865\u5e8f\u5217\uff0c\u5219\u4f7f\u7528revcomp\u3002\u547d\u4ee4\u5982\u4e0b\uff1a<\/span>    <\/span><o:page><\/o:page><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">samtools view test.bam | bioawk -c sam '{s=$seq; if(and($flag, 16)) {s=revcomp($seq)} print \">\"$qname\"n\"s}'<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">\u7ed3\u679c\u6587\u4ef6\uff1a<\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.2222222222222222\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_GHkTiaxxzlPfN4IHZfEIL6FTFMAtIw-1.png?resize=553%2C122\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOSuSibmrSKibndA5XFGsTN4292icJGHkTiaxxzlPfN4IHZfEIL6FTFMAtIw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"122 .87001037597656\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;font-variant: normal;text-transform: none;\">          <\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;font-variant-numeric: normal;font-variant-east-asian: normal;\">\u603b\u4e4b\uff0cbioawk\u662f\u4e00\u4e2a\u975e\u5e38\u5f3a\u5927\u7684\u6587\u672c\u5904\u7406\u5de5\u5177\uff0c\u5b83\u53ef\u4ee5\u5e2e\u52a9\u6211\u4eec\u5feb\u901f\u5730\u5904\u7406\u5404\u79cd\u751f\u7269\u4fe1\u606f\u5b66\u76f8\u5173\u7684\u6570\u636e\u683c\u5f0f\u3002\u5b83\u7684\u8bed\u6cd5\u7b80\u5355\u6613\u5b66\uff0c\u529f\u80fd\u5f3a\u5927\uff0c\u53ef\u4ee5\u8ba9\u6211\u4eec\u8f7b\u677e\u5730\u5b8c\u6210\u5404\u79cd\u590d\u6742\u7684\u6570\u636e\u5206\u6790\u4efb\u52a1\u3002\u5982\u679c\u60a8\u662f\u4e00\u540d\u751f\u7269\u4fe1\u606f\u5b66\u5de5\u4f5c\u8005\uff0c\u90a3\u4e48bioawk\u4e00\u5b9a\u662f\u60a8\u4e0d\u53ef\u6216\u7f3a\u7684\u5de5\u5177\u4e4b\u4e00\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;text-indent: 0em;letter-spacing: 0.034em;font-variant-numeric: normal;font-variant-east-asian: normal;background: rgb(255, 255, 255);\">\u5e0c\u671b\u8fd9\u7bc7\u6587\u7ae0\u80fd\u591f\u5e2e\u52a9\u4f60\u63d0\u9ad8\u4f60\u7684\u6587\u672c\u5904\u7406\u80fd\u529b\uff0c\u8ba9\u4f60\u5728\u751f\u7269\u4fe1\u606f\u5b66\u4e2d\u66f4\u52a0\u6e38\u5203\u6709\u4f59\u3002\u5982\u679c\u4f60\u60f3\u8981\u4e86\u89e3\u66f4\u591a\u5173\u4e8ebioawk\u7684\u4fe1\u606f\uff0c\u4f60\u53ef\u4ee5\u8bbf\u95ee\u5b83\u7684GitHub\u9875\u9762\uff0c\u6216\u8005\u67e5\u770b\u5b83\u7684\u624b\u518c\u3002\u5982\u679c\u4f60\u6709\u4efb\u4f55\u95ee\u9898\u6216\u8005\u5efa\u8bae\uff0c\u6b22\u8fce\u5728\u8bc4\u8bba\u533a\u7559\u8a00\u6216\u8005\u8054\u7cfb\u6211\u3002\u8c22\u8c22\u4f60\u7684\u9605\u8bfb\uff0c\u4e0b\u6b21\u518d\u89c1\u3002<\/span><\/span><\/section>\n<section style=\"text-align: justify;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-variant: normal;text-transform: none;background: rgb(255, 255, 255);font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><o:p><\/o:p><\/span><\/section>\n<section style=\"text-align: left;margin-left: 0pt;font-size: 12pt;font-family: \u5b8b\u4f53;font-weight: normal;line-height: 150%;text-indent: 0em;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);font-size: 15px;\">\u53c2\u8003\u6587\u6863\uff1a<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-variant: normal;text-transform: none;background: rgb(255, 255, 255);font-size: 15px;\">https:\/\/github.com\/lh3\/bioawk\/blob\/master\/README.md<\/span><\/span><\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);text-align: left;\">\n<section style=\"outline: 0px;font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;outline: 0px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;outline: 0px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;outline: 0px;\">\n<section style=\"outline: 0px;display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 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href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"outline: 0px;cursor: pointer;font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 122, 170);\" rel=\"noopener\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 122, 170);\"><\/span><\/a><\/section>\n<section style=\"margin-bottom: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica 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rel=\"noopener\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/a><br style=\"outline: 0px;\" \/><\/section>\n<section style=\"margin-bottom: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 0.578px;white-space: normal;background-color: rgb(255, 255, 255);outline: 0px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" 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imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" rel=\"noopener\">3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8<\/a><\/section>\n<section style=\"margin-bottom: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 0.578px;white-space: normal;background-color: rgb(255, 255, 255);outline: 0px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491905&idx=1&sn=fb34ebd6282962c7803badaee7a39096&chksm=cf7f7184f808f8929608c0050344ad86193d0fc6455c1c18e16cc17bf4b37832b63d3b18284e&scene=21#wechat_redirect\" 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