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powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 10px;padding-left: 10px;line-height: 1.8;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u5c0f\u4f19\u4f34\u4eec\u5927\u5bb6\u597d\uff0c\u6211\u662f\u5927\u6d77\u54e5\uff0c\u4eca\u5929\u8981\u7ed9\u5927\u5bb6\u5206\u4eab\u7684\u4e0d\u662f\u4ec0\u4e48\u5f88\u5389\u5bb3\u7684\u6280\u672f\uff0c\u4e5f\u4e0d\u662f\u8d85\u524d\u7684\u7406\u8bba\u79d1\u666e\uff0c\u800c\u662f\u4e00\u4e2a\u6bcf\u4e2a\u751f\u4fe1\u4eba\u90fd\u80fd\u7528\u5f97\u4e0a\u7684\u5c0f\u6280\u5de7\uff0c\u4f60\u8bf4\u4f60\u7528\u4e0d\u4e0a\uff1f\u96be\u9053\u4f60\u4e0d\u505a\u5dee\u5f02\u5206\u6790\uff1f\u53ea\u8981\u4f60\u9700\u8981\u505a\u5dee\u5f02\u5206\u6790\uff0c\u90a3\u4e48\u4f60\u80af\u5b9a\u4f1a\u7ed8\u5236\u706b\u5c71\u56fe\uff0c\u4e8e\u662f\u4eca\u5929\u5927\u6d77\u54e5\u5206\u4eab\u7684\u6280\u5de7\u4f60\u5c31\u53ef\u4ee5\u7528\u4e0a\u4e86\u3002\u662f\u7684\u6ca1\u9519~\u5927\u6d77\u54e5\u4eca\u5929\u5206\u4eab\u7684\u5c31\u662f\u706b\u5c71\u56fe\u7ed8\u5236\u7684\u5c0f\u6280\u5de7\uff0c\u7ed9\u57fa\u56e0\u6563\u70b9\u52a0\u4e0a\u57fa\u56e0\u540d\u6807\u7b7e\u3002<\/p>\n<\/section>\n<section style=\"font-size: 11px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5148\u51c6\u5907\u597dR\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span>(!require(ggplot2)) <\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggplot2\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span>(!require(ggrepel)) <\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggrepel\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span>(!require(dplyr))<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"dplyr\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggrepel)<\/span><\/code><code><span class=\"code-snippet_outer\">library(dplyr)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 11px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u672c\u6b21\u6211\u4eec\u67093\u79cd\u65b9\u53ef\u4ee5\u5b9e\u73b0\u6563\u70b9\u6dfb\u52a0\u6807\u7b7e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u65b9\u6cd5\u4e00\uff1a\u5229\u7528\u7a7a\u5b57\u7b26\u201c\u201d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u79cd\u65b9\u6cd5\u662f\u53c2\u7167\u5e2e\u52a9\u6587\u6863\u91cc\u7684\u4e00\u6bb5\u4ee3\u7801\uff1a<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e0b\u9762\u4ee3\u7801\u6765\u6e90\u4e8egeom_text_repel\u7684\u5e2e\u52a9\u6587\u6863<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p <- ggplot(mtcars,aes(wt, mpg, label = rownames(mtcars), colour = factor(cyl))) + geom_point()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Hide some of the labels, but repel from all data points<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">mtcars$label <- rownames(mtcars)<\/span><\/code><code><span class=\"code-snippet_outer\">mtcars$label[<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">15<\/span>] <- <span class=\"code-snippet__string\">\"\"<\/span>p + geom_text_repel(data = mtcars, aes(wt, mpg, label = label))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 11px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7625649913344887\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_DMrGQ8Tib8m068c1U1TpFXdEO1uUPw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPDV8W1x4kGoxr1VCMnn4ibHouk3DMrGQ8Tib8m068c1U1TpFXdEO1uUPw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"577\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u662f\u7ed3\u679c\u56fe\uff0c\u53ef\u4ee5\u770b\u5230\uff0c\u4e00\u4e9b\u70b9\u6709\u6807\u7b7e\u540d\uff0c\u4e00\u4e9b\u6ca1\u6709<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e8e\u662f\u5927\u6d77\u54e5\u5b66\u4ee5\u81f4\u7528<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5148\u753b\u51fa\u706b\u5c71\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p <- ggplot(data = data, aes(x = log2(data$FC),<\/span><\/code><code><span class=\"code-snippet_outer\">y = -log10(data$p.value),colour=change,label = data$symbol)) +<\/span><\/code><code><span class=\"code-snippet_outer\">geom_point(alpha=<span class=\"code-snippet__number\">0.4<\/span>, size=<span class=\"code-snippet__number\">3.5<\/span>) + scale_color_manual(values=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"blue\"<\/span>, <span class=\"code-snippet__string\">\"grey\"<\/span>,<span class=\"code-snippet__string\">\"red\"<\/span>))+<\/span><\/code><code><span class=\"code-snippet_outer\">xlim(<span class=\"code-snippet__built_in\">c<\/span>(-<span class=\"code-snippet__number\">4.5<\/span>, <span class=\"code-snippet__number\">4.5<\/span>)) +geom_vline(xintercept=<span class=\"code-snippet__built_in\">c<\/span>(-<span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">1<\/span>),lty=<span class=\"code-snippet__number\">4<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.8<\/span>) +geom_hline(yintercept = -log10(<span class=\"code-snippet__number\">0.000001<\/span>),lty=<span class=\"code-snippet__number\">4<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">labs(x=<span class=\"code-snippet__string\">\"log2(fold change)\"<\/span>,y=<span class=\"code-snippet__string\">\"-log10 (p-value)\"<\/span>,title=<span class=\"code-snippet__string\">\"Differential metabolites\"<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw()+ theme(plot.title = element_text(hjust = <span class=\"code-snippet__number\">0.5<\/span>), legend.position=<span class=\"code-snippet__string\">\"right\"<\/span>,legend.title = element_blank())<\/span><\/code><code><span class=\"code-snippet_outer\">p<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5601851851851852\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_gUd6umMIGKwU1Sficvkicl8mpaEJPA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPWtFIk4XlIzfDiaesbBNibWW46qtVgUd6umMIGKwU1Sficvkicl8mpaEJPA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7136\u540e\u6211\u4eec\u53ef\u4ee5\u7b5b\u9009\u51fa\u90e8\u5206\u57fa\u56e0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u60f3\u5728\u56fe\u4e0a\u505a\u4fee\u6539\uff0c\u4e00\u534a\u662f\u8c03\u53c2\u6570\uff0c\u4e00\u534a\u662f\u8c03\u6570\u636e\u3002\u6211\u4eec\u73b0\u5728\u8981\u505a\u7684\u5c31\u662f\u8c03\u6570\u636e\uff1a\u8981\u6807\u8bb0\u7684\uff0clabel=\u57fa\u56e0\uff0c\u65e0\u9700\u6807\u8bb0\u7684\uff0clabel=\u201c\u201d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data$label=ifelse(data$p.value < <span class=\"code-snippet__number\">0.000001<\/span> & <span class=\"code-snippet__built_in\">abs<\/span>(log2(data$FC)) >= <span class=\"code-snippet__number\">1<\/span>,data$symbol,<span class=\"code-snippet__string\">\"\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u5c31\u597d\u4e86\uff0c\u7136\u540e\u628a\u6587\u5b57\u56fe\u5c42\u53e0\u52a0\u4e0a\u53bb<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#data\u4e3a\u5dee\u5f02\u5206\u6790\u7ed3\u679c\uff0c\u5927\u5bb6\u81ea\u884c\u4fee\u6539\u54e6\uff01\u5305\u542bFC\u5217\u548cp.value\u4ee5\u53ca\u57fa\u56e0\u540d\u5217\u5c31\u53ef\u4ee5\u5566\uff01<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p+geom_text_repel(data = data, aes(x = log2(data$FC), y = -log10(data$p.value), label = label),<\/span><\/code><code><span class=\"code-snippet_outer\">size = <span class=\"code-snippet__number\">3<\/span>,box.padding = unit(<span class=\"code-snippet__number\">0.5<\/span>, <span class=\"code-snippet__string\">\"lines\"<\/span>),point.padding = unit(<span class=\"code-snippet__number\">0.8<\/span>, <span class=\"code-snippet__string\">\"lines\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">segment.color = <span class=\"code-snippet__string\">\"black\"<\/span>,show.legend = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5583333333333333\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_VRgia6KjqdicQTf8CPheAicI53CcfA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPePNzkLRmf6yC2BpAMhyqoTPgOQjVRgia6KjqdicQTf8CPheAicI53CcfA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">##\u5b8c\u7f8e\u5b9e\u73b0\uff01\u4f46\u662f\u8fd9\u79cd\u529e\u6cd5\u53ea\u9002\u7528\u4e8e\u6570\u636e\u91cf\u8f83\u5c0f\u7684\u65f6\u5019\uff0c\u6570\u636e\u91cf\u592a\u5927\u4e86\u5bf9\u4e8e\u8bbe\u5907\u7684\u6027\u80fd\u8981\u6c42\u5c31\u4f1a\u8981\u6c42\u592a\u9ad8\u4e86\uff01<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u65b9\u6cd5\u4e8c\uff1a\u56fe\u5c42\u53e0\u52a0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u4e2a\u65b9\u6cd5\u662f\u6765\u81eaR\u6570\u636e\u79d1\u5b66\u7b14\u8bb0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">best_in_class <- mpg %>% group_by(<span class=\"code-snippet__built_in\">class<\/span>) %>% filter(row_number(desc(hwy)) == <span class=\"code-snippet__number\">1<\/span>)ggplot(mpg, aes(displ, hwy)) + geom_point(aes(color = <span class=\"code-snippet__built_in\">class<\/span>)) + geom_point(size = <span class=\"code-snippet__number\">3<\/span>, shape = <span class=\"code-snippet__number\">1<\/span>, data = best_in_class) + ggrepel::geom_label_repel( aes(label = model), data = best_in_class )<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5344352617079889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_35MP4JS8eukQF45ZykspCSTdv2gUdg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPaI5rGGngnmcL8ibhfFLt9QKLR35MP4JS8eukQF45ZykspCSTdv2gUdg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"726\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u4e2a\u65b9\u6cd5\u9002\u7528\u4e8e\u8f83\u5927\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5148\u540c\u6837\u753b\u51fa\u706b\u5c71\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p <- ggplot(data = test,aes(x = logFC,y = P.value)) + <span class=\"code-snippet__comment\">#test\u662f\u5dee\u5f02\u5206\u6790\u7ed3\u679c\uff0c\u5927\u5bb6\u81ea\u884c\u66f4\u6362\u54e6\uff0c\u5305\u542blogFC\u5217\u548cP.value\u4ee5\u53ca\u57fa\u56e0\u540d\u5217\u5c31\u53ef\u4ee5\u5566\uff01<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">geom_point(alpha=<span class=\"code-snippet__number\">0.4<\/span>, size=<span class=\"code-snippet__number\">3.5<\/span>,aes(color=change)) +<\/span><\/code><code><span class=\"code-snippet_outer\">scale_color_manual(values=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"blue\"<\/span>, <span class=\"code-snippet__string\">\"grey\"<\/span>,<span class=\"code-snippet__string\">\"red\"<\/span>))+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_vline(xintercept=<span class=\"code-snippet__built_in\">c<\/span>(-<span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">1<\/span>),lty=<span class=\"code-snippet__number\">4<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">geom_hline(yintercept = -log10(<span class=\"code-snippet__number\">0.01<\/span>),lty=<span class=\"code-snippet__number\">4<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw()<\/span><\/code><code><span class=\"code-snippet_outer\">p<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5344352617079889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_afficaPcztcgfO5670h1bVdfurLvnw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPAC5vCvgIpibhGmAwm1NpvxP8IyiafficaPcztcgfO5670h1bVdfurLvnw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"726\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7136\u540e\u9009\u62e9\u60f3\u6807\u8bb0\u7684\u57fa\u56e0\uff0c\u65b0\u5efa\u4e00\u4e2a\u6807\u7b7e\u56fe\u5c42<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">for_label <- test %>% filter(<span class=\"code-snippet__built_in\">abs<\/span>(logFC) ><span class=\"code-snippet__number\">4<\/span>& P.value> -log10(<span class=\"code-snippet__number\">0.000001<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u518d\u628a\u6807\u7b7e\u56fe\u5c42\u53e0\u52a0\u4e0a\u53bb<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p + geom_point(size = <span class=\"code-snippet__number\">3<\/span>, shape = <span class=\"code-snippet__number\">1<\/span>, data = for_label) +ggrepel::geom_label_repel(aes(label = symbol),data = for_label, color=<span class=\"code-snippet__string\">\"black\"<\/span> )<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 11px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"558\" data-cropsely1=\"0\" data-cropsely2=\"298\" data-ratio=\"0.6469719350073855\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_a96vfOvClPhjtnezoicWn5YWaVEuOA.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPCayxgZaXgRP9ZJkd96JCTLic2fa96vfOvClPhjtnezoicWn5YWaVEuOA\/640?wx_fmt=jpeg'\" data-type=\"png\" data-w=\"677\" style=\"vertical-align: middle;width: 558px;height: 361px;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u8fd9\u4e2a\u770b\u7740\u4e5f\u662f\u76f8\u5f53\u4e0d\u9519\uff01<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd8\u6709\u65b9\u6cd5\u4e09\uff0c\u4e5f\u662f\u6700\u4fbf\u6377\u7684\u4e00\u79cd\u65b9\u5f0f\uff0c\u56e0\u4e3aggpubr\u7684\u51fd\u6570\u6709\u73b0\u6210\u7684\u53c2\u6570<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr) colnames(test)[<span class=\"code-snippet__number\">4<\/span>] <- <span class=\"code-snippet__string\">\"v\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggscatter(test,x = <span class=\"code-snippet__string\">\"logFC\"<\/span>,y =<span class=\"code-snippet__string\">\"P.value\"<\/span>, ylab=<span class=\"code-snippet__string\">\"-log10(P.value)\"<\/span>,size=<span class=\"code-snippet__number\">0.5<\/span>,color = <span class=\"code-snippet__string\">\"change\"<\/span>, palette = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"#00AFBB\"<\/span>, <span class=\"code-snippet__string\">\"#999999\"<\/span>, <span class=\"code-snippet__string\">\"#FC4E07\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7136\u540e\u53ef\u4ee5\u624b\u52a8\u9009\u62e9\u6211\u4eec\u60f3\u8981\u6807\u8bb0\u7684\u57fa\u56e0\uff0c\u8fd9\u91cc\u5927\u6d77\u54e5\u5217\u4e3e\u4e86\u8fd9\u51e0\u4e2a\"CD36\", \"DUSP6\", \"DCT\", \"SPRY2\", \"MOXD1\", \"ETV4\"\uff0c\u5927\u5bb6\u81ea\u884c\u4fee\u6539\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggscatter(test, x = <span class=\"code-snippet__string\">\"logFC\"<\/span>, y = <span class=\"code-snippet__string\">\"v\"<\/span>, ylab=<span class=\"code-snippet__string\">\"-log10(P.value)\"<\/span>, color = <span class=\"code-snippet__string\">\"change\"<\/span>, size = <span class=\"code-snippet__number\">0.5<\/span>, label = <span class=\"code-snippet__string\">\"symbol\"<\/span>, repel = <span class=\"code-snippet__built_in\">T<\/span>, palette = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"#00AFBB\"<\/span>, <span class=\"code-snippet__string\">\"#999999\"<\/span>, <span class=\"code-snippet__string\">\"#FC4E07\"<\/span>) , <\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c31\u662f\u8fd9\u4e2alabel.select\u6807\u7b7e\uff0c\u76f4\u63a5\u52a0\u4e0a\u53bb\u5c31\u884c\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#label.select = dat$symbol[1:30] , label.select = c(\"CD36\", \"DUSP6\", \"DCT\", \"SPRY2\", \"MOXD1\", \"ETV4\" ) )<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5148\u770b\u770b\u539f\u59cb\u7684\u56fe<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6469719350073855\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_YQmnM2z4hKGmx0pYgREw9ib76iaSHA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyP2Jn7JKYeljx555Eom0WqsJoXVRYQmnM2z4hKGmx0pYgREw9ib76iaSHA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"677\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\"><br \/><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u770b\u770b\u52a0\u4e0a\u540e\u7684\u56fe\uff0c\u6211\u4eec\u8fd9\u91cc\u91cd\u65b0\u9009\u62e9logFC\u524d30\u4e2a\u57fa\u56e0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggscatter(test,x = <span class=\"code-snippet__string\">\"logFC\"<\/span>,y = <span class=\"code-snippet__string\">\"v\"<\/span>,ylab=<span class=\"code-snippet__string\">\"-log10(P.value)\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">color = <span class=\"code-snippet__string\">\"change\"<\/span>,size = <span class=\"code-snippet__number\">0.5<\/span>,label = <span class=\"code-snippet__string\">\"symbol\"<\/span>,repel = <span class=\"code-snippet__built_in\">T<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">palette = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"#00AFBB\"<\/span>, <span class=\"code-snippet__string\">\"#999999\"<\/span>, <span class=\"code-snippet__string\">\"#FC4E07\"<\/span>) ,<\/span><\/code><code><span class=\"code-snippet_outer\">label.select = test$symbol[<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">30<\/span>])<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6787807737397421\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_gVNZycbOpQqNSuEm0hs9cu84iaBbnQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPLKZfWwEU9COulvwWIIzkaeMJAgVNZycbOpQqNSuEm0hs9cu84iaBbnQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"853\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u53ef\u4ee5\u770b\u5230\u7ed3\u679c\u5341\u5206\u7684\u65b9\u4fbf\u7f8e\u89c2\uff01<\/p>\n<\/section>\n<section style=\"display: flex;flex-flow: row nowrap;margin: 10px 0% 20px;text-align: left;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;align-self: flex-start;line-height: 0;\">\n<section style=\"text-align: right;margin-right: 0%;margin-bottom: -20px;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 20px;vertical-align: top;overflow: hidden;border-width: 0px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 241, 224);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 20px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 21px 21px 0px 0px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 100 100 0%;align-self: flex-start;height: auto;border-right: 0px none rgb(62, 62, 62);border-top-right-radius: 12px;overflow: hidden;box-shadow: rgb(231, 215, 173) 0px 6px 0px;\">\n<section style=\"margin: 20px 0% 30px;\" powered-by=\"xiumi.us\">\n<section style=\"padding-right: 10px;padding-left: 10px;text-align: justify;line-height: 1.8;\">\n<p style=\"white-space: normal;\">\u5927\u6d77\u54e5\u6765\u603b\u7ed3\u4e00\u4e0b\uff0c\u706b\u5c71\u56fe\u662f\u4e00\u79cd\u5e38\u7528\u4e8e\u57fa\u56e0\u8868\u8fbe\u5206\u6790\u4e2d\u7684\u53ef\u89c6\u5316\u5de5\u5177\uff0c\u7528\u4e8e\u5c55\u793a\u4e24\u4e2a\u6761\u4ef6\u6216\u7ec4\u4e4b\u95f4\u7684\u5dee\u5f02\u6027\u3002\u4e00\u822c\u6211\u4eec\u90fd\u4f1a\u5728\u5dee\u5f02\u5206\u6790\u4e4b\u540e\uff0c\u4f7f\u7528\u706b\u5c71\u56fe\u6765\u5c55\u793a\u5dee\u5f02\u7ed3\u679c\uff0c\u90a3\u4e48\u4eca\u5929\u5728\u5b66\u4f1a\u4e86\u5927\u6d77\u54e5\u7684\u5c0f\u6280\u5de7\u6559\u5b66\u540e\uff0c\u5c0f\u4f19\u4f34\u4eec\u7684\u706b\u5c71\u56fe\u80af\u5b9a\u662f\u5982\u864e\u6dfb\u7ffc\uff0c\u8bba\u6587\u5206\u503c\u80af\u5b9a\u8e6d\u8e6d\u5f80\u4e0a\u6da8\uff0c\u4e0d\u8fc7\uff0c\u8fd8\u662f\u8981\u5148\u8001\u8001\u5b9e\u5b9e\u5730\u52a8\u624b\u8054\u7cfb\u4e00\u4e0b\u54e6\uff01\u8bf4\u4e0d\u5b9a\u4f60\u753b\u51fa\u7684\u56fe\u6bd4\u5927\u6d77\u54e5\u7684\u66f4\u52a0\u4f18\u79c0\uff01\u5feb\u52a8\u624b\u8bd5\u8bd5\u5427\uff01\uff08\u6700\u540e\u63a8\u8350\u4e00\u4e0b\u5927\u6d77\u54e5\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u4e91\u751f\u4fe1\u5206\u6790\u5de5\u5177\u5e73\u53f0\u5305\u542b\u8d85\u591a\u96f6\u4ee3\u7801\u5c0f\u5de5\u5177\uff0c\u4e0a\u4f20\u6570\u636e\u4e00\u952e\u51fa\u56fe\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u53c2\u89c2\u54df\uff0c\u7f51\u5740\uff1ahttp:\/\/www.biocloudservice.com\/home.html\uff09<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section 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powered-by=\"xiumi.us\">\n<p>\u70b9\u51fb\u201c\u9605\u8bfb\u539f\u6587\u201d\u8fdb\u5165\u7f51\u5740<\/p>\n<\/section>\n<section style=\"margin-left: 8%;margin-right: 8%;line-height: 1.1em;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;vertical-align: top;border-top: 1em solid rgb(0, 150, 136);border-left: 0.8em solid transparent !important;border-right: 0.8em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/mp.weixin.qq.com\/s\/rIxuSQtNg_eOOxLm2oNpiQ#rd\" target=\"_blank\" 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[…]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57274"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=57274"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57274\/revisions"}],"predecessor-version":[{"id":57285,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57274\/revisions\/57285"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=57274"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=57274"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=57274"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

{"id":57274,"date":"2024-06-16T19:03:10","date_gmt":"2024-06-16T11:03:10","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=57274"},"modified":"2024-06-16T19:03:10","modified_gmt":"2024-06-16T11:03:10","slug":"%e5%ae%9e%e7%94%a8%e5%b0%8f%e6%8a%80%e5%b7%a7-%e7%bb%99%e7%81%ab%e5%b1%b1%e5%9b%be%e6%b7%bb%e5%8a%a0%e5%9f%ba%e5%9b%a0%e6%a0%87%e7%ad%be","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=57274","title":{"rendered":"\u5b9e\u7528\u5c0f\u6280\u5de7\u2014\u2014\u7ed9\u706b\u5c71\u56fe\u6dfb\u52a0\u57fa\u56e0\u6807\u7b7e"},"content":{"rendered":"

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powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 10px;padding-left: 10px;line-height: 1.8;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u5c0f\u4f19\u4f34\u4eec\u5927\u5bb6\u597d\uff0c\u6211\u662f\u5927\u6d77\u54e5\uff0c\u4eca\u5929\u8981\u7ed9\u5927\u5bb6\u5206\u4eab\u7684\u4e0d\u662f\u4ec0\u4e48\u5f88\u5389\u5bb3\u7684\u6280\u672f\uff0c\u4e5f\u4e0d\u662f\u8d85\u524d\u7684\u7406\u8bba\u79d1\u666e\uff0c\u800c\u662f\u4e00\u4e2a\u6bcf\u4e2a\u751f\u4fe1\u4eba\u90fd\u80fd\u7528\u5f97\u4e0a\u7684\u5c0f\u6280\u5de7\uff0c\u4f60\u8bf4\u4f60\u7528\u4e0d\u4e0a\uff1f\u96be\u9053\u4f60\u4e0d\u505a\u5dee\u5f02\u5206\u6790\uff1f\u53ea\u8981\u4f60\u9700\u8981\u505a\u5dee\u5f02\u5206\u6790\uff0c\u90a3\u4e48\u4f60\u80af\u5b9a\u4f1a\u7ed8\u5236\u706b\u5c71\u56fe\uff0c\u4e8e\u662f\u4eca\u5929\u5927\u6d77\u54e5\u5206\u4eab\u7684\u6280\u5de7\u4f60\u5c31\u53ef\u4ee5\u7528\u4e0a\u4e86\u3002\u662f\u7684\u6ca1\u9519~\u5927\u6d77\u54e5\u4eca\u5929\u5206\u4eab\u7684\u5c31\u662f\u706b\u5c71\u56fe\u7ed8\u5236\u7684\u5c0f\u6280\u5de7\uff0c\u7ed9\u57fa\u56e0\u6563\u70b9\u52a0\u4e0a\u57fa\u56e0\u540d\u6807\u7b7e\u3002<\/p>\n<\/section>\n<section style=\"font-size: 11px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5148\u51c6\u5907\u597dR\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span>(!require(ggplot2)) <\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggplot2\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span>(!require(ggrepel)) <\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggrepel\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span>(!require(dplyr))<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"dplyr\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggrepel)<\/span><\/code><code><span class=\"code-snippet_outer\">library(dplyr)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 11px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u672c\u6b21\u6211\u4eec\u67093\u79cd\u65b9\u53ef\u4ee5\u5b9e\u73b0\u6563\u70b9\u6dfb\u52a0\u6807\u7b7e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u65b9\u6cd5\u4e00\uff1a\u5229\u7528\u7a7a\u5b57\u7b26\u201c\u201d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u79cd\u65b9\u6cd5\u662f\u53c2\u7167\u5e2e\u52a9\u6587\u6863\u91cc\u7684\u4e00\u6bb5\u4ee3\u7801\uff1a<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e0b\u9762\u4ee3\u7801\u6765\u6e90\u4e8egeom_text_repel\u7684\u5e2e\u52a9\u6587\u6863<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p <- ggplot(mtcars,aes(wt, mpg, label = rownames(mtcars), colour = factor(cyl))) + geom_point()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Hide some of the labels, but repel from all data points<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">mtcars$label <- rownames(mtcars)<\/span><\/code><code><span class=\"code-snippet_outer\">mtcars$label[<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">15<\/span>] <- <span class=\"code-snippet__string\">\"\"<\/span>p + geom_text_repel(data = mtcars, aes(wt, mpg, label = label))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 11px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7625649913344887\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_DMrGQ8Tib8m068c1U1TpFXdEO1uUPw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPDV8W1x4kGoxr1VCMnn4ibHouk3DMrGQ8Tib8m068c1U1TpFXdEO1uUPw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"577\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u662f\u7ed3\u679c\u56fe\uff0c\u53ef\u4ee5\u770b\u5230\uff0c\u4e00\u4e9b\u70b9\u6709\u6807\u7b7e\u540d\uff0c\u4e00\u4e9b\u6ca1\u6709<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e8e\u662f\u5927\u6d77\u54e5\u5b66\u4ee5\u81f4\u7528<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5148\u753b\u51fa\u706b\u5c71\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p <- ggplot(data = data, aes(x = log2(data$FC),<\/span><\/code><code><span class=\"code-snippet_outer\">y = -log10(data$p.value),colour=change,label = data$symbol)) +<\/span><\/code><code><span class=\"code-snippet_outer\">geom_point(alpha=<span class=\"code-snippet__number\">0.4<\/span>, size=<span class=\"code-snippet__number\">3.5<\/span>) + scale_color_manual(values=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"blue\"<\/span>, <span class=\"code-snippet__string\">\"grey\"<\/span>,<span class=\"code-snippet__string\">\"red\"<\/span>))+<\/span><\/code><code><span class=\"code-snippet_outer\">xlim(<span class=\"code-snippet__built_in\">c<\/span>(-<span class=\"code-snippet__number\">4.5<\/span>, <span class=\"code-snippet__number\">4.5<\/span>)) +geom_vline(xintercept=<span class=\"code-snippet__built_in\">c<\/span>(-<span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">1<\/span>),lty=<span class=\"code-snippet__number\">4<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.8<\/span>) +geom_hline(yintercept = -log10(<span class=\"code-snippet__number\">0.000001<\/span>),lty=<span class=\"code-snippet__number\">4<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">labs(x=<span class=\"code-snippet__string\">\"log2(fold change)\"<\/span>,y=<span class=\"code-snippet__string\">\"-log10 (p-value)\"<\/span>,title=<span class=\"code-snippet__string\">\"Differential metabolites\"<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw()+ theme(plot.title = element_text(hjust = <span class=\"code-snippet__number\">0.5<\/span>), legend.position=<span class=\"code-snippet__string\">\"right\"<\/span>,legend.title = element_blank())<\/span><\/code><code><span class=\"code-snippet_outer\">p<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5601851851851852\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_gUd6umMIGKwU1Sficvkicl8mpaEJPA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPWtFIk4XlIzfDiaesbBNibWW46qtVgUd6umMIGKwU1Sficvkicl8mpaEJPA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7136\u540e\u6211\u4eec\u53ef\u4ee5\u7b5b\u9009\u51fa\u90e8\u5206\u57fa\u56e0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u60f3\u5728\u56fe\u4e0a\u505a\u4fee\u6539\uff0c\u4e00\u534a\u662f\u8c03\u53c2\u6570\uff0c\u4e00\u534a\u662f\u8c03\u6570\u636e\u3002\u6211\u4eec\u73b0\u5728\u8981\u505a\u7684\u5c31\u662f\u8c03\u6570\u636e\uff1a\u8981\u6807\u8bb0\u7684\uff0clabel=\u57fa\u56e0\uff0c\u65e0\u9700\u6807\u8bb0\u7684\uff0clabel=\u201c\u201d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data$label=ifelse(data$p.value < <span class=\"code-snippet__number\">0.000001<\/span> & <span class=\"code-snippet__built_in\">abs<\/span>(log2(data$FC)) >= <span class=\"code-snippet__number\">1<\/span>,data$symbol,<span class=\"code-snippet__string\">\"\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u5c31\u597d\u4e86\uff0c\u7136\u540e\u628a\u6587\u5b57\u56fe\u5c42\u53e0\u52a0\u4e0a\u53bb<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#data\u4e3a\u5dee\u5f02\u5206\u6790\u7ed3\u679c\uff0c\u5927\u5bb6\u81ea\u884c\u4fee\u6539\u54e6\uff01\u5305\u542bFC\u5217\u548cp.value\u4ee5\u53ca\u57fa\u56e0\u540d\u5217\u5c31\u53ef\u4ee5\u5566\uff01<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p+geom_text_repel(data = data, aes(x = log2(data$FC), y = -log10(data$p.value), label = label),<\/span><\/code><code><span class=\"code-snippet_outer\">size = <span class=\"code-snippet__number\">3<\/span>,box.padding = unit(<span class=\"code-snippet__number\">0.5<\/span>, <span class=\"code-snippet__string\">\"lines\"<\/span>),point.padding = unit(<span class=\"code-snippet__number\">0.8<\/span>, <span class=\"code-snippet__string\">\"lines\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">segment.color = <span class=\"code-snippet__string\">\"black\"<\/span>,show.legend = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5583333333333333\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_VRgia6KjqdicQTf8CPheAicI53CcfA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPePNzkLRmf6yC2BpAMhyqoTPgOQjVRgia6KjqdicQTf8CPheAicI53CcfA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">##\u5b8c\u7f8e\u5b9e\u73b0\uff01\u4f46\u662f\u8fd9\u79cd\u529e\u6cd5\u53ea\u9002\u7528\u4e8e\u6570\u636e\u91cf\u8f83\u5c0f\u7684\u65f6\u5019\uff0c\u6570\u636e\u91cf\u592a\u5927\u4e86\u5bf9\u4e8e\u8bbe\u5907\u7684\u6027\u80fd\u8981\u6c42\u5c31\u4f1a\u8981\u6c42\u592a\u9ad8\u4e86\uff01<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u65b9\u6cd5\u4e8c\uff1a\u56fe\u5c42\u53e0\u52a0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u4e2a\u65b9\u6cd5\u662f\u6765\u81eaR\u6570\u636e\u79d1\u5b66\u7b14\u8bb0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">best_in_class <- mpg %>% group_by(<span class=\"code-snippet__built_in\">class<\/span>) %>% filter(row_number(desc(hwy)) == <span class=\"code-snippet__number\">1<\/span>)ggplot(mpg, aes(displ, hwy)) + geom_point(aes(color = <span class=\"code-snippet__built_in\">class<\/span>)) + geom_point(size = <span class=\"code-snippet__number\">3<\/span>, shape = <span class=\"code-snippet__number\">1<\/span>, data = best_in_class) + ggrepel::geom_label_repel( aes(label = model), data = best_in_class )<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5344352617079889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_35MP4JS8eukQF45ZykspCSTdv2gUdg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPaI5rGGngnmcL8ibhfFLt9QKLR35MP4JS8eukQF45ZykspCSTdv2gUdg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"726\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u4e2a\u65b9\u6cd5\u9002\u7528\u4e8e\u8f83\u5927\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5148\u540c\u6837\u753b\u51fa\u706b\u5c71\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p <- ggplot(data = test,aes(x = logFC,y = P.value)) + <span class=\"code-snippet__comment\">#test\u662f\u5dee\u5f02\u5206\u6790\u7ed3\u679c\uff0c\u5927\u5bb6\u81ea\u884c\u66f4\u6362\u54e6\uff0c\u5305\u542blogFC\u5217\u548cP.value\u4ee5\u53ca\u57fa\u56e0\u540d\u5217\u5c31\u53ef\u4ee5\u5566\uff01<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">geom_point(alpha=<span class=\"code-snippet__number\">0.4<\/span>, size=<span class=\"code-snippet__number\">3.5<\/span>,aes(color=change)) +<\/span><\/code><code><span class=\"code-snippet_outer\">scale_color_manual(values=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"blue\"<\/span>, <span class=\"code-snippet__string\">\"grey\"<\/span>,<span class=\"code-snippet__string\">\"red\"<\/span>))+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_vline(xintercept=<span class=\"code-snippet__built_in\">c<\/span>(-<span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">1<\/span>),lty=<span class=\"code-snippet__number\">4<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">geom_hline(yintercept = -log10(<span class=\"code-snippet__number\">0.01<\/span>),lty=<span class=\"code-snippet__number\">4<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw()<\/span><\/code><code><span class=\"code-snippet_outer\">p<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5344352617079889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_afficaPcztcgfO5670h1bVdfurLvnw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPAC5vCvgIpibhGmAwm1NpvxP8IyiafficaPcztcgfO5670h1bVdfurLvnw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"726\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7136\u540e\u9009\u62e9\u60f3\u6807\u8bb0\u7684\u57fa\u56e0\uff0c\u65b0\u5efa\u4e00\u4e2a\u6807\u7b7e\u56fe\u5c42<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">for_label <- test %>% filter(<span class=\"code-snippet__built_in\">abs<\/span>(logFC) ><span class=\"code-snippet__number\">4<\/span>& P.value> -log10(<span class=\"code-snippet__number\">0.000001<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u518d\u628a\u6807\u7b7e\u56fe\u5c42\u53e0\u52a0\u4e0a\u53bb<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p + geom_point(size = <span class=\"code-snippet__number\">3<\/span>, shape = <span class=\"code-snippet__number\">1<\/span>, data = for_label) +ggrepel::geom_label_repel(aes(label = symbol),data = for_label, color=<span class=\"code-snippet__string\">\"black\"<\/span> )<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 11px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"558\" data-cropsely1=\"0\" data-cropsely2=\"298\" data-ratio=\"0.6469719350073855\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_a96vfOvClPhjtnezoicWn5YWaVEuOA.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPCayxgZaXgRP9ZJkd96JCTLic2fa96vfOvClPhjtnezoicWn5YWaVEuOA\/640?wx_fmt=jpeg'\" data-type=\"png\" data-w=\"677\" style=\"vertical-align: middle;width: 558px;height: 361px;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u8fd9\u4e2a\u770b\u7740\u4e5f\u662f\u76f8\u5f53\u4e0d\u9519\uff01<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd8\u6709\u65b9\u6cd5\u4e09\uff0c\u4e5f\u662f\u6700\u4fbf\u6377\u7684\u4e00\u79cd\u65b9\u5f0f\uff0c\u56e0\u4e3aggpubr\u7684\u51fd\u6570\u6709\u73b0\u6210\u7684\u53c2\u6570<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr) colnames(test)[<span class=\"code-snippet__number\">4<\/span>] <- <span class=\"code-snippet__string\">\"v\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggscatter(test,x = <span class=\"code-snippet__string\">\"logFC\"<\/span>,y =<span class=\"code-snippet__string\">\"P.value\"<\/span>, ylab=<span class=\"code-snippet__string\">\"-log10(P.value)\"<\/span>,size=<span class=\"code-snippet__number\">0.5<\/span>,color = <span class=\"code-snippet__string\">\"change\"<\/span>, palette = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"#00AFBB\"<\/span>, <span class=\"code-snippet__string\">\"#999999\"<\/span>, <span class=\"code-snippet__string\">\"#FC4E07\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7136\u540e\u53ef\u4ee5\u624b\u52a8\u9009\u62e9\u6211\u4eec\u60f3\u8981\u6807\u8bb0\u7684\u57fa\u56e0\uff0c\u8fd9\u91cc\u5927\u6d77\u54e5\u5217\u4e3e\u4e86\u8fd9\u51e0\u4e2a\"CD36\", \"DUSP6\", \"DCT\", \"SPRY2\", \"MOXD1\", \"ETV4\"\uff0c\u5927\u5bb6\u81ea\u884c\u4fee\u6539\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggscatter(test, x = <span class=\"code-snippet__string\">\"logFC\"<\/span>, y = <span class=\"code-snippet__string\">\"v\"<\/span>, ylab=<span class=\"code-snippet__string\">\"-log10(P.value)\"<\/span>, color = <span class=\"code-snippet__string\">\"change\"<\/span>, size = <span class=\"code-snippet__number\">0.5<\/span>, label = <span class=\"code-snippet__string\">\"symbol\"<\/span>, repel = <span class=\"code-snippet__built_in\">T<\/span>, palette = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"#00AFBB\"<\/span>, <span class=\"code-snippet__string\">\"#999999\"<\/span>, <span class=\"code-snippet__string\">\"#FC4E07\"<\/span>) , <\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c31\u662f\u8fd9\u4e2alabel.select\u6807\u7b7e\uff0c\u76f4\u63a5\u52a0\u4e0a\u53bb\u5c31\u884c\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#label.select = dat$symbol[1:30] , label.select = c(\"CD36\", \"DUSP6\", \"DCT\", \"SPRY2\", \"MOXD1\", \"ETV4\" ) )<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5148\u770b\u770b\u539f\u59cb\u7684\u56fe<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6469719350073855\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_YQmnM2z4hKGmx0pYgREw9ib76iaSHA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyP2Jn7JKYeljx555Eom0WqsJoXVRYQmnM2z4hKGmx0pYgREw9ib76iaSHA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"677\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\"><br \/><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u770b\u770b\u52a0\u4e0a\u540e\u7684\u56fe\uff0c\u6211\u4eec\u8fd9\u91cc\u91cd\u65b0\u9009\u62e9logFC\u524d30\u4e2a\u57fa\u56e0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggscatter(test,x = <span class=\"code-snippet__string\">\"logFC\"<\/span>,y = <span class=\"code-snippet__string\">\"v\"<\/span>,ylab=<span class=\"code-snippet__string\">\"-log10(P.value)\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">color = <span class=\"code-snippet__string\">\"change\"<\/span>,size = <span class=\"code-snippet__number\">0.5<\/span>,label = <span class=\"code-snippet__string\">\"symbol\"<\/span>,repel = <span class=\"code-snippet__built_in\">T<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">palette = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"#00AFBB\"<\/span>, <span class=\"code-snippet__string\">\"#999999\"<\/span>, <span class=\"code-snippet__string\">\"#FC4E07\"<\/span>) ,<\/span><\/code><code><span class=\"code-snippet_outer\">label.select = test$symbol[<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">30<\/span>])<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6787807737397421\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_gVNZycbOpQqNSuEm0hs9cu84iaBbnQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSQTqNwz8s6dfN2Dm85XQXyPLKZfWwEU9COulvwWIIzkaeMJAgVNZycbOpQqNSuEm0hs9cu84iaBbnQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"853\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u53ef\u4ee5\u770b\u5230\u7ed3\u679c\u5341\u5206\u7684\u65b9\u4fbf\u7f8e\u89c2\uff01<\/p>\n<\/section>\n<section style=\"display: flex;flex-flow: row nowrap;margin: 10px 0% 20px;text-align: left;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;align-self: flex-start;line-height: 0;\">\n<section style=\"text-align: right;margin-right: 0%;margin-bottom: -20px;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 20px;vertical-align: top;overflow: hidden;border-width: 0px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 241, 224);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 20px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 21px 21px 0px 0px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 100 100 0%;align-self: flex-start;height: auto;border-right: 0px none rgb(62, 62, 62);border-top-right-radius: 12px;overflow: hidden;box-shadow: rgb(231, 215, 173) 0px 6px 0px;\">\n<section style=\"margin: 20px 0% 30px;\" powered-by=\"xiumi.us\">\n<section style=\"padding-right: 10px;padding-left: 10px;text-align: justify;line-height: 1.8;\">\n<p style=\"white-space: normal;\">\u5927\u6d77\u54e5\u6765\u603b\u7ed3\u4e00\u4e0b\uff0c\u706b\u5c71\u56fe\u662f\u4e00\u79cd\u5e38\u7528\u4e8e\u57fa\u56e0\u8868\u8fbe\u5206\u6790\u4e2d\u7684\u53ef\u89c6\u5316\u5de5\u5177\uff0c\u7528\u4e8e\u5c55\u793a\u4e24\u4e2a\u6761\u4ef6\u6216\u7ec4\u4e4b\u95f4\u7684\u5dee\u5f02\u6027\u3002\u4e00\u822c\u6211\u4eec\u90fd\u4f1a\u5728\u5dee\u5f02\u5206\u6790\u4e4b\u540e\uff0c\u4f7f\u7528\u706b\u5c71\u56fe\u6765\u5c55\u793a\u5dee\u5f02\u7ed3\u679c\uff0c\u90a3\u4e48\u4eca\u5929\u5728\u5b66\u4f1a\u4e86\u5927\u6d77\u54e5\u7684\u5c0f\u6280\u5de7\u6559\u5b66\u540e\uff0c\u5c0f\u4f19\u4f34\u4eec\u7684\u706b\u5c71\u56fe\u80af\u5b9a\u662f\u5982\u864e\u6dfb\u7ffc\uff0c\u8bba\u6587\u5206\u503c\u80af\u5b9a\u8e6d\u8e6d\u5f80\u4e0a\u6da8\uff0c\u4e0d\u8fc7\uff0c\u8fd8\u662f\u8981\u5148\u8001\u8001\u5b9e\u5b9e\u5730\u52a8\u624b\u8054\u7cfb\u4e00\u4e0b\u54e6\uff01\u8bf4\u4e0d\u5b9a\u4f60\u753b\u51fa\u7684\u56fe\u6bd4\u5927\u6d77\u54e5\u7684\u66f4\u52a0\u4f18\u79c0\uff01\u5feb\u52a8\u624b\u8bd5\u8bd5\u5427\uff01\uff08\u6700\u540e\u63a8\u8350\u4e00\u4e0b\u5927\u6d77\u54e5\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u4e91\u751f\u4fe1\u5206\u6790\u5de5\u5177\u5e73\u53f0\u5305\u542b\u8d85\u591a\u96f6\u4ee3\u7801\u5c0f\u5de5\u5177\uff0c\u4e0a\u4f20\u6570\u636e\u4e00\u952e\u51fa\u56fe\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u53c2\u89c2\u54df\uff0c\u7f51\u5740\uff1ahttp:\/\/www.biocloudservice.com\/home.html\uff09<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section 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powered-by=\"xiumi.us\">\n<p>\u70b9\u51fb\u201c\u9605\u8bfb\u539f\u6587\u201d\u8fdb\u5165\u7f51\u5740<\/p>\n<\/section>\n<section style=\"margin-left: 8%;margin-right: 8%;line-height: 1.1em;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;vertical-align: top;border-top: 1em solid rgb(0, 150, 136);border-left: 0.8em solid transparent !important;border-right: 0.8em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/mp.weixin.qq.com\/s\/rIxuSQtNg_eOOxLm2oNpiQ#rd\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/mp.weixin.qq.com\/s\/rIxuSQtNg_eOOxLm2oNpiQ#rd\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5b9e\u7528\u5c0f\u6280\u5de7\u2014\u2014\u7ed9\u706b\u5c71\u56fe\u6dfb\u52a0\u57fa\u56e0\u6807\u7b7e \u5927\u6d77\u54e5 \u751f\u4fe1\u679c 2023-08 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57274"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=57274"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57274\/revisions"}],"predecessor-version":[{"id":57285,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57274\/revisions\/57285"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=57274"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=57274"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=57274"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}