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rgb(249, 172, 172) 0px 0px 5px 3px;\">\n<section style=\"text-align: center;transform: translate3d(2px, 0px, 0px);margin-top: -14px;margin-bottom: -14px;\" powered-by=\"xiumi.us\">\n<p>{ \u70b9\u51fb\u84dd\u5b57\uff0c\u5173\u6ce8\u6211\u4eec }<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"color: rgb(249, 110, 87);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u4ec0\u4e48\u662f\u5dee\u5f02\u5206\u6790<\/strong><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">\u5dee\u5f02\u5206\u6790\uff0c\u6bd4\u8f83\u4e24\u7ec4\u7684\u5dee\u5f02\u6027\u505a\u76f8\u5173\u5206\u6790\u3002\u5728\u6bd4\u8f83\u7684\u4e24\u7ec4\u7684\u6570\u636e\u8fc7\u7a0b\u4e2d\uff0c\u6d89\u53ca\u5230\u201c\u663e\u8457\u201d\u7684\u5b9a\u4e49\u3002\u600e\u6837\u7684\u5dee\u5f02\u624d\u7b97\u662f\u663e\u8457\u7684\u5462\uff0c\u8fd9\u5c31\u9700\u8981\u6211\u4eec\u4e86\u89e3\u4e00\u4e9b\u7edf\u8ba1\u5b66\u7684\u77e5\u8bc6\u4e86\u3002\u901a\u5e38\u6211\u4eec\u662f\u5bf9\u4e24\u7ec4\u6570\u636e\u7684\u5dee\u5f02\u500d\u6570\u8fdb\u884c\u7edf\u8ba1\u5b66\u4e0a\u7684\u68c0\u9a8c\uff0c\u5f97\u5230P value\uff0c\u8ba4\u4e3a\u8fbe\u5230\u4e00\u5b9a\u7684\u9608\u503c\uff08p<0.05\uff09\u5219\u4e3a\u663e\u8457\u5dee\u5f02\u3002\u6211\u4eec\u7ecf\u5e38\u505a\u5dee\u5f02\u68c0\u9a8c\u7684\u65f6\u5019\u770b\u4e00\u4e9b\u7edf\u8ba1\u5b66\u7684\u540d\u8bcd\uff0c\u4f8b\u5982\uff1a<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\"><strong>FC\uff08Fold Change\uff09<\/strong>\uff0c\u5373\u4e3a\u5dee\u5f02\u500d\u6570\u3002\u7b80\u5355\u6765\u8bf4\u5c31\u662f\u57fa\u56e0\u5728\u4e00\u7ec4\u6837\u54c1\u4e2d\u7684\u8868\u8fbe\u503c\u7684\u5747\u503c\u9664\u4ee5\u5176\u5728\u53e6\u4e00\u7ec4\u6837\u54c1\u4e2d\u7684\u8868\u8fbe\u503c\u7684\u5747\u503c\u3002\u5982\u679c\u89c4\u5b9aFC>2\u4e3a\u4e0a\u8c03\uff0c\u90a3\u4e48FC<1\/2\u4e3a\u4e0b\u8c03\u3002\u5728\u5f88\u591a\u5b9e\u9645\u5e94\u7528\u4e2d\uff0c\u5e38\u5e38\u6709\u4eba\u628aFC\u503c\u505alog2\u8f6c\u6362\uff0clog2fc\u7684\u503c\u6709\u6b63\u8d1f\u503c\u4e4b\u5206\uff0c\u5f88\u5bb9\u6613\u770b\u51fa2\u4e2agroup\u4e4b\u95f4\u7684\u4e0a\u4e0b\u8c03\u5173\u7cfb\u3002<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\"><strong>Pvalue<\/strong>\u7edf\u8ba1\u68c0\u9a8c\u83b7\u5f97\u7684\u662f\u5426\u7edf\u8ba1\u5dee\u5f02\u663e\u8457\u7684\u4e00\u4e2a\u8861\u91cf\u503c\uff0c\u7ea6\u5b9a\u6210\u4fd7\u7684P-value<0.05\u4e3a\u7edf\u8ba1\u68c0\u9a8c\u663e\u8457\u7684\u5e38\u89c4\u6807\u51c6\u3002<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\"><strong>FDR (false discovery rate)<\/strong>\uff0c\u5373\u6821\u6b63\u540e\u7684P\u503c\uff0c\u4e2d\u6587\u4e00\u822c\u8bd1\u4f5c\u9519\u8bef\u53d1\u73b0\u7387\u3002<\/p>\n<\/section>\n<section style=\"color: rgb(249, 110, 87);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u5dee\u5f02\u5206\u6790\u7684\u65b9\u6cd5<\/strong><\/p>\n<\/section>\n<p style=\"text-indent: 0em;white-space: normal;\" powered-by=\"xiumi.us\">\u5e38\u89c1\u7684\u5dee\u5f02\u6027\u5206\u6790\u65b9\u6cd5\u6709\uff0cT\u68c0\u9a8c\u3001\u65b9\u5dee\u5206\u6790\u3001\u5361\u65b9\u68c0\u9a8c\u3002\u6839\u636e\u4f60\u7684\u6570\u636e\u7c7b\u578b\u9009\u62e9\u5408\u9002\u7684\u5dee\u5f02\u5206\u6790\u7684\u65b9\u6cd5\uff0c\u5982\u679cX\u548cY\u6570\u636e\u90fd\u662f\u5b9a\u7c7b\u7684\u6570\u636e\u7c7b\u578b\u5c31\u9700\u8981\u9002\u5e94\u5361\u65b9\u68c0\u9a8c\uff0c\u5982\u679cX\u662f\u5b9a\u7c7b\uff0cY\u662f\u5b9a\u91cf\u7684\u6570\u636e\u7c7b\u578b\uff0c\u90a3\u53ef\u4ee5\u4f7f\u7528T\u68c0\u9a8c\u6216\u8005\u662f\u65b9\u5dee\u68c0\u9a8c\u3002T\u68c0\u9a8c\u9002\u5408\u4e8eX\u7ec4\u522b\u662f2\u7684\u6570\u636e\uff0c\u800c\u65b9\u5dee\u68c0\u9a8c\u9002\u7528\u4e8e2\u7ec4\u6216\u4e24\u7ec4\u4ee5\u4e0a\u7684\u3002<\/p>\n<section style=\"color: rgb(249, 110, 87);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u5dee\u5f02\u5206\u6790\u7684\u4f5c\u56fe<\/strong><\/p>\n<\/section>\n<p style=\"text-indent: 0em;white-space: normal;\" powered-by=\"xiumi.us\">\u4e00\u822c\u5dee\u5f02\u5206\u6790\u53ef\u4ee5\u7528\u591a\u79cd\u7c7b\u578b\u7684\u56fe\u6765\u5c55\u793a\uff0c\u6709\u7bb1\u7ebf\u56fe\u3001\u5c0f\u63d0\u7434\u56fe\u3001\u504f\u5dee\u56fe\u548c\u706b\u5c71\u56fe\u7b49\u7b49\u3002\u4eca\u5929\u6211\u4eec\u7528R\u5305\u6765\u63a2\u7d22\u706b\u5c71\u56fe\u6765\u5c55\u793a\u5dee\u5f02\u5206\u6790\u7684\u7ed3\u679c\u3002<\/p>\n<section style=\"color: rgb(249, 110, 87);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u6784\u5efa\u6570\u636e\u96c6<\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#set.seed(1234)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">mydata <- data.frame(<\/span><\/code><code><span class=\"code-snippet_outer\">Genes = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Gene1\"<\/span>,<span class=\"code-snippet__string\">\"Gene2\"<\/span>,<span class=\"code-snippet__string\">\"Gene3\"<\/span>,<span class=\"code-snippet__string\">\"Gene4\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">treat1 = rnorm(<span class=\"code-snippet__number\">4<\/span>,<span class=\"code-snippet__number\">2<\/span>)+<span class=\"code-snippet__number\">1<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">treat2 = rnorm(<span class=\"code-snippet__number\">4<\/span>,<span class=\"code-snippet__number\">2<\/span>)+<span class=\"code-snippet__number\">1<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">control1 = rnorm(<span class=\"code-snippet__number\">4<\/span>,<span class=\"code-snippet__number\">1.5<\/span>)+<span class=\"code-snippet__number\">1<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">control1 = rnorm(<span class=\"code-snippet__number\">4<\/span>,<span class=\"code-snippet__number\">1.5<\/span>)+<span class=\"code-snippet__number\">1<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><code><span class=\"code-snippet_outer\">mydata <span class=\"code-snippet__comment\">#\u521b\u5efa\u6837\u672c\u8868\u8fbe\u91cf\u4fe1\u606f<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.28450704225352114\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_2xrj64iadGLzrHOPl3iaiczTgxFZIA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVf1VJJZOUxwO2pKGibibNzWNaLYyASLkVQLtfXFgYDJs6ozW5Zy2xrj64iadGLzrHOPl3iaiczTgxFZIA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"710\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">group <- data.frame(Sample = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"treat1\"<\/span>, <span class=\"code-snippet__string\">\"treat2\"<\/span>,<span class=\"code-snippet__string\">\"control1\"<\/span>,<span class=\"code-snippet__string\">\"control2\"<\/span>),group = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__string\">\"treat\"<\/span>,<span class=\"code-snippet__number\">2<\/span>),<span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__string\">\"control\"<\/span>,<span class=\"code-snippet__number\">2<\/span>)))<\/span><\/code><code><span class=\"code-snippet_outer\">group<span class=\"code-snippet__comment\">#\u521b\u5efa\u7684\u6837\u672c\u5206\u7ec4\u4fe1\u606f<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4163424124513619\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_rUwy4vw1VxObhJRj3OEiaLcpcUT5eQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVf1VJJZOUxwO2pKGibibNzWNuwlatLGugQIOHhHpdcN4lVMKRrUwy4vw1VxObhJRj3OEiaLcpcUT5eQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"514\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong><span style=\"color: rgb(249, 110, 87);\">\u6570\u636e\u5206\u6790<\/span><\/strong><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8868\u8fbe\u91cf+\u5206\u7ec4 \u5408\u5e76\uff0c\u6574\u7406<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"tidyverse\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"rstatix\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">library(tidyverse)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">library(rstatix)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6570\u636e\u7684\u6574\u5408<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">df <- mydata %>% as_tibble() %>% pivot_longer(-<span class=\"code-snippet__number\">1<\/span>,names_to = <span class=\"code-snippet__string\">\"Sample\"<\/span>,values_to = <span class=\"code-snippet__string\">\"value\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6dfb\u52a0\u5206\u7ec4\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">df <- left_join(df,group,by=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Sample\"<\/span> = <span class=\"code-snippet__string\">\"Sample\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5233918128654971\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_Mp3qxZBs48nS2icvJ7t2lTSpFUhFcA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVf1VJJZOUxwO2pKGibibNzWNEYaJBpzg7v5TD6VPachHKI86OMp3qxZBs48nS2icvJ7t2lTSpFUhFcA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"684\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"color: rgb(249, 110, 87);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u7ed8\u5236\u706b\u5c71\u56fe<\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u91cc\u628a\u6d4b\u8bd5\u7684\u6837\u672c\u653e\u5728\u9644\u4ef6\u4e2d\u5566\uff0c\u4f9b\u53c2\u8003<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dataset <- read.table(file = <span class=\"code-snippet__string\">\".\/dataset_volcano.txt\"<\/span>, header = <span class=\"code-snippet__literal\">TRUE<\/span>, sep = <span class=\"code-snippet__string\">\"\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8bbe\u7f6ep_value\u548clogFC\u7684\u9608\u503c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cut_off_pvalue = <span class=\"code-snippet__number\">0.0000001<\/span> <span class=\"code-snippet__comment\">#\u7edf\u8ba1\u663e\u8457\u6027<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cut_off_logFC = <span class=\"code-snippet__number\">1.5<\/span> <span class=\"code-snippet__comment\">#\u5dee\u5f02\u500d\u6570\u503c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6839\u636e\u9608\u503c\u53c2\u6570\uff0c\u4e0a\u8c03\u57fa\u56e0\u8bbe\u7f6e\u4e3a\u2018up\u2019\uff0c\u4e0b\u8c03\u57fa\u56e0\u8bbe\u7f6e\u4e3a\u2018Down\u2019\uff0c\u65e0\u5dee\u5f02\u8bbe\u7f6e\u4e3a\u2018Stable\u2019\uff0c\u5e76\u4fdd\u5b58\u5230change\u5217\u4e2d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dataset$change = ifelse(dataset$P.Value < cut_off_pvalue & <span class=\"code-snippet__built_in\">abs<\/span>(dataset$logFC) >= cut_off_logFC, <\/span><\/code><code><span class=\"code-snippet_outer\"> ifelse(dataset$logFC> cut_off_logFC ,<span class=\"code-snippet__string\">'Up'<\/span>,<span class=\"code-snippet__string\">'Down'<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__string\">'Stable'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">head(dataset)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3486682808716707\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_ukvgpJ9JsU58YhibGia1QiceeIrWjA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVf1VJJZOUxwO2pKGibibNzWNZXQjOa2QUMrPFj50RDtFgbu9nuDukvgpJ9JsU58YhibGia1QiceeIrWjA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"826\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">p <- ggplot(<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\"># \u6570\u636e\u3001\u6620\u5c04\u3001\u989c\u8272<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> dataset, aes(x = logFC, y = -log10(P.Value), colour=change)) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(alpha=<span class=\"code-snippet__number\">0.8<\/span>, size=<span class=\"code-snippet__number\">1.5<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_color_manual(values=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"blue\"<\/span>, <span class=\"code-snippet__string\">\"#d2dae2\"<\/span>,<span class=\"code-snippet__string\">\"red\"<\/span>))+<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\"># \u8f85\u52a9\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> geom_vline(xintercept=<span class=\"code-snippet__built_in\">c<\/span>(-<span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">1<\/span>),lty=<span class=\"code-snippet__number\">4<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_hline(yintercept = -log10(cut_off_pvalue),lty=<span class=\"code-snippet__number\">4<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\"># \u5750\u6807\u8f74<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> labs(x=<span class=\"code-snippet__string\">\"log2(fold change)\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> y=<span class=\"code-snippet__string\">\"-log10 (p-value)\"<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\"> theme_bw()+<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\"># \u56fe\u4f8b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> theme(plot.title = element_text(hjust = <span class=\"code-snippet__number\">0.5<\/span>), <\/span><\/code><code><span class=\"code-snippet_outer\"> legend.position=<span class=\"code-snippet__string\">\"right\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> legend.title = element_blank())<\/span><\/code><code><span class=\"code-snippet_outer\">p<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6dfb\u52a0\u6807\u7b7e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u5c06\u9700\u8981\u6807\u8bb0\u7684\u57fa\u56e0\u653e\u7f6e\u5728label\u5217(logFC >= 5)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(ggrepel)<\/span><\/code><code><span class=\"code-snippet_outer\">dataset$label <- ifelse(dataset$P.Value > cut_off_pvalue & <span class=\"code-snippet__built_in\">abs<\/span>(dataset$logFC) >= <span class=\"code-snippet__number\">5<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__built_in\">as.character<\/span>(dataset$gene), <span class=\"code-snippet__string\">\"\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">p + geom_label_repel(data = dataset, aes(x = dataset$logFC, <\/span><\/code><code><span class=\"code-snippet_outer\"> y = -log10(dataset$P.Value), <\/span><\/code><code><span class=\"code-snippet_outer\"> label = label),<\/span><\/code><code><span class=\"code-snippet_outer\"> size = <span class=\"code-snippet__number\">3<\/span>, box.padding = unit(<span class=\"code-snippet__number\">0.5<\/span>, <span class=\"code-snippet__string\">\"lines\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> point.padding = unit(<span class=\"code-snippet__number\">0.1<\/span>, <span class=\"code-snippet__string\">\"lines\"<\/span>), <\/span><\/code><code><span class=\"code-snippet_outer\"> segment.color = <span class=\"code-snippet__string\">\"black\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> show.legend = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8851851851851852\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_qdIFF5djt2WEUUClpAj5v49Labdbfg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVf1VJJZOUxwO2pKGibibNzWNnq09icKkqorSyamy2mIibAYOWibqdIFF5djt2WEUUClpAj5v49Labdbfg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"color: rgb(249, 110, 87);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u7406\u89e3\u706b\u5c71\u56fe<\/strong><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u706b\u5c71\u56fe\u662f\u6563\u70b9\u56fe\u7684\u4e00\u79cd\uff0c\u5b83\u5c06\u7edf\u8ba1\u6d4b\u8bd5\u4e2d\u7684\u7edf\u8ba1\u663e\u8457\u6027\u91cf\u5ea6\uff08\u5982p value\uff09\u548c\u53d8\u5316\u5e45\u5ea6\u76f8\u7ed3\u5408\uff0c\u4ece\u800c\u80fd\u591f\u5e2e\u52a9\u5feb\u901f\u76f4\u89c2\u5730\u8bc6\u522b\u90a3\u4e9b\u53d8\u5316\u5e45\u5ea6\u8f83\u5927\u4e14\u5177\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\u7684\u6570\u636e\u70b9\uff08\u57fa\u56e0\u7b49\uff09\u3002\u5e38\u5e94\u7528\u4e8e\u8f6c\u5f55\u7ec4\u7814\u7a76\uff0c\u4e5f\u80fd\u5e94\u7528\u4e8e\u57fa\u56e0\u7ec4\uff0c\u86cb\u767d\u8d28\u7ec4\uff0c\u4ee3\u8c22\u7ec4\u7b49\u7edf\u8ba1\u6570\u636e\u3002\u5173\u6ce8\u706b\u5c71\u56fe\uff0c\u5148\u7406\u89e3\u6bcf\u4e2a\u70b9\u662f\u4ec0\u4e48\uff08\u70b9\u4ee3\u8868\u57fa\u56e0\u3001\u6837\u54c1\u3001\u901a\u8def\u6216\u5176\u5b83\u7684\uff0c\u8fd9\u4e2a\u8ba4\u8bc6\u53ef\u4ee5\u6765\u81ea\u4e8e\u5e38\u8bc6\uff0c\u66f4\u51c6\u786e\u7684\u662f\u770b\u4f5c\u8005\u7684\u63cf\u8ff0\uff09\uff0c\u7136\u540e\u770b\u6a2a\u8f74\u4ee3\u8868\u4ec0\u4e48\u3001\u7eb5\u8f74\u4ee3\u8868\u4ec0\u4e48\uff0c\u518d\u770b\u56fe\u4f8b\u4e2d\u5c55\u793a\u7684\u5176\u4ed6\u4fe1\u606f\uff0c\u5982\u989c\u8272\u3001\u5927\u5c0f\u548c\u5f62\u72b6\u5206\u522b\u4ee3\u8868\u4ec0\u4e48\u3002\u8fd9\u4e9b\u90fd\u7406\u987a\u4e86\uff0c\u56fe\u7406\u89e3\u5c31\u4e0d\u96be\u4e86\u3002<\/p>\n<p 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data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u5dee\u5f02\u5206\u6790\u2014\u2014\u4ece\u539f\u7406\u5230\u706b\u5c71\u56fe\u7684\u5b9e\u73b0\",\"time\":\"2023-06-29 19:00:39\",\"timeStamp\":\"1688036439\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488882&idx=2&sn=00d6ad04d3d063b35d4f49bbc9c29de0&chksm=cf7c85b7f80b0ca10bfb6fcad21e923831e8e446dfa1100a7376bfff15365e44484b4c0ba1f7#rd\" target=\"_blank\" rel=\"noopener\">\u5dee\u5f02\u5206\u6790\u2014\u2014\u4ece\u539f\u7406\u5230\u706b\u5c71\u56fe\u7684\u5b9e\u73b0<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u5e08\u59b9 <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" 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rgb(249, 172, 172) 0px 0px 5px 3px;\">\n<section style=\"text-align: center;transform: translate3d(2px, 0px, 0px);margin-top: -14px;margin-bottom: -14px;\" powered-by=\"xiumi.us\">\n<p>{ \u70b9\u51fb\u84dd\u5b57\uff0c\u5173\u6ce8\u6211\u4eec }<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"color: rgb(249, 110, 87);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u4ec0\u4e48\u662f\u5dee\u5f02\u5206\u6790<\/strong><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">\u5dee\u5f02\u5206\u6790\uff0c\u6bd4\u8f83\u4e24\u7ec4\u7684\u5dee\u5f02\u6027\u505a\u76f8\u5173\u5206\u6790\u3002\u5728\u6bd4\u8f83\u7684\u4e24\u7ec4\u7684\u6570\u636e\u8fc7\u7a0b\u4e2d\uff0c\u6d89\u53ca\u5230\u201c\u663e\u8457\u201d\u7684\u5b9a\u4e49\u3002\u600e\u6837\u7684\u5dee\u5f02\u624d\u7b97\u662f\u663e\u8457\u7684\u5462\uff0c\u8fd9\u5c31\u9700\u8981\u6211\u4eec\u4e86\u89e3\u4e00\u4e9b\u7edf\u8ba1\u5b66\u7684\u77e5\u8bc6\u4e86\u3002\u901a\u5e38\u6211\u4eec\u662f\u5bf9\u4e24\u7ec4\u6570\u636e\u7684\u5dee\u5f02\u500d\u6570\u8fdb\u884c\u7edf\u8ba1\u5b66\u4e0a\u7684\u68c0\u9a8c\uff0c\u5f97\u5230P value\uff0c\u8ba4\u4e3a\u8fbe\u5230\u4e00\u5b9a\u7684\u9608\u503c\uff08p<0.05\uff09\u5219\u4e3a\u663e\u8457\u5dee\u5f02\u3002\u6211\u4eec\u7ecf\u5e38\u505a\u5dee\u5f02\u68c0\u9a8c\u7684\u65f6\u5019\u770b\u4e00\u4e9b\u7edf\u8ba1\u5b66\u7684\u540d\u8bcd\uff0c\u4f8b\u5982\uff1a<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\"><strong>FC\uff08Fold Change\uff09<\/strong>\uff0c\u5373\u4e3a\u5dee\u5f02\u500d\u6570\u3002\u7b80\u5355\u6765\u8bf4\u5c31\u662f\u57fa\u56e0\u5728\u4e00\u7ec4\u6837\u54c1\u4e2d\u7684\u8868\u8fbe\u503c\u7684\u5747\u503c\u9664\u4ee5\u5176\u5728\u53e6\u4e00\u7ec4\u6837\u54c1\u4e2d\u7684\u8868\u8fbe\u503c\u7684\u5747\u503c\u3002\u5982\u679c\u89c4\u5b9aFC>2\u4e3a\u4e0a\u8c03\uff0c\u90a3\u4e48FC<1\/2\u4e3a\u4e0b\u8c03\u3002\u5728\u5f88\u591a\u5b9e\u9645\u5e94\u7528\u4e2d\uff0c\u5e38\u5e38\u6709\u4eba\u628aFC\u503c\u505alog2\u8f6c\u6362\uff0clog2fc\u7684\u503c\u6709\u6b63\u8d1f\u503c\u4e4b\u5206\uff0c\u5f88\u5bb9\u6613\u770b\u51fa2\u4e2agroup\u4e4b\u95f4\u7684\u4e0a\u4e0b\u8c03\u5173\u7cfb\u3002<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\"><strong>Pvalue<\/strong>\u7edf\u8ba1\u68c0\u9a8c\u83b7\u5f97\u7684\u662f\u5426\u7edf\u8ba1\u5dee\u5f02\u663e\u8457\u7684\u4e00\u4e2a\u8861\u91cf\u503c\uff0c\u7ea6\u5b9a\u6210\u4fd7\u7684P-value<0.05\u4e3a\u7edf\u8ba1\u68c0\u9a8c\u663e\u8457\u7684\u5e38\u89c4\u6807\u51c6\u3002<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\"><strong>FDR (false discovery rate)<\/strong>\uff0c\u5373\u6821\u6b63\u540e\u7684P\u503c\uff0c\u4e2d\u6587\u4e00\u822c\u8bd1\u4f5c\u9519\u8bef\u53d1\u73b0\u7387\u3002<\/p>\n<\/section>\n<section style=\"color: rgb(249, 110, 87);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u5dee\u5f02\u5206\u6790\u7684\u65b9\u6cd5<\/strong><\/p>\n<\/section>\n<p style=\"text-indent: 0em;white-space: normal;\" powered-by=\"xiumi.us\">\u5e38\u89c1\u7684\u5dee\u5f02\u6027\u5206\u6790\u65b9\u6cd5\u6709\uff0cT\u68c0\u9a8c\u3001\u65b9\u5dee\u5206\u6790\u3001\u5361\u65b9\u68c0\u9a8c\u3002\u6839\u636e\u4f60\u7684\u6570\u636e\u7c7b\u578b\u9009\u62e9\u5408\u9002\u7684\u5dee\u5f02\u5206\u6790\u7684\u65b9\u6cd5\uff0c\u5982\u679cX\u548cY\u6570\u636e\u90fd\u662f\u5b9a\u7c7b\u7684\u6570\u636e\u7c7b\u578b\u5c31\u9700\u8981\u9002\u5e94\u5361\u65b9\u68c0\u9a8c\uff0c\u5982\u679cX\u662f\u5b9a\u7c7b\uff0cY\u662f\u5b9a\u91cf\u7684\u6570\u636e\u7c7b\u578b\uff0c\u90a3\u53ef\u4ee5\u4f7f\u7528T\u68c0\u9a8c\u6216\u8005\u662f\u65b9\u5dee\u68c0\u9a8c\u3002T\u68c0\u9a8c\u9002\u5408\u4e8eX\u7ec4\u522b\u662f2\u7684\u6570\u636e\uff0c\u800c\u65b9\u5dee\u68c0\u9a8c\u9002\u7528\u4e8e2\u7ec4\u6216\u4e24\u7ec4\u4ee5\u4e0a\u7684\u3002<\/p>\n<section style=\"color: rgb(249, 110, 87);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u5dee\u5f02\u5206\u6790\u7684\u4f5c\u56fe<\/strong><\/p>\n<\/section>\n<p style=\"text-indent: 0em;white-space: normal;\" powered-by=\"xiumi.us\">\u4e00\u822c\u5dee\u5f02\u5206\u6790\u53ef\u4ee5\u7528\u591a\u79cd\u7c7b\u578b\u7684\u56fe\u6765\u5c55\u793a\uff0c\u6709\u7bb1\u7ebf\u56fe\u3001\u5c0f\u63d0\u7434\u56fe\u3001\u504f\u5dee\u56fe\u548c\u706b\u5c71\u56fe\u7b49\u7b49\u3002\u4eca\u5929\u6211\u4eec\u7528R\u5305\u6765\u63a2\u7d22\u706b\u5c71\u56fe\u6765\u5c55\u793a\u5dee\u5f02\u5206\u6790\u7684\u7ed3\u679c\u3002<\/p>\n<section style=\"color: rgb(249, 110, 87);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u6784\u5efa\u6570\u636e\u96c6<\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#set.seed(1234)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">mydata <- data.frame(<\/span><\/code><code><span class=\"code-snippet_outer\">Genes = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Gene1\"<\/span>,<span class=\"code-snippet__string\">\"Gene2\"<\/span>,<span class=\"code-snippet__string\">\"Gene3\"<\/span>,<span class=\"code-snippet__string\">\"Gene4\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">treat1 = rnorm(<span class=\"code-snippet__number\">4<\/span>,<span class=\"code-snippet__number\">2<\/span>)+<span class=\"code-snippet__number\">1<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">treat2 = rnorm(<span class=\"code-snippet__number\">4<\/span>,<span class=\"code-snippet__number\">2<\/span>)+<span class=\"code-snippet__number\">1<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">control1 = rnorm(<span class=\"code-snippet__number\">4<\/span>,<span class=\"code-snippet__number\">1.5<\/span>)+<span class=\"code-snippet__number\">1<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">control1 = rnorm(<span class=\"code-snippet__number\">4<\/span>,<span class=\"code-snippet__number\">1.5<\/span>)+<span class=\"code-snippet__number\">1<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">)<\/span><\/code><code><span class=\"code-snippet_outer\">mydata <span class=\"code-snippet__comment\">#\u521b\u5efa\u6837\u672c\u8868\u8fbe\u91cf\u4fe1\u606f<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.28450704225352114\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_2xrj64iadGLzrHOPl3iaiczTgxFZIA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVf1VJJZOUxwO2pKGibibNzWNaLYyASLkVQLtfXFgYDJs6ozW5Zy2xrj64iadGLzrHOPl3iaiczTgxFZIA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"710\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">group <- data.frame(Sample = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"treat1\"<\/span>, <span class=\"code-snippet__string\">\"treat2\"<\/span>,<span class=\"code-snippet__string\">\"control1\"<\/span>,<span class=\"code-snippet__string\">\"control2\"<\/span>),group = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__string\">\"treat\"<\/span>,<span class=\"code-snippet__number\">2<\/span>),<span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__string\">\"control\"<\/span>,<span class=\"code-snippet__number\">2<\/span>)))<\/span><\/code><code><span class=\"code-snippet_outer\">group<span class=\"code-snippet__comment\">#\u521b\u5efa\u7684\u6837\u672c\u5206\u7ec4\u4fe1\u606f<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4163424124513619\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_rUwy4vw1VxObhJRj3OEiaLcpcUT5eQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVf1VJJZOUxwO2pKGibibNzWNuwlatLGugQIOHhHpdcN4lVMKRrUwy4vw1VxObhJRj3OEiaLcpcUT5eQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"514\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong><span style=\"color: rgb(249, 110, 87);\">\u6570\u636e\u5206\u6790<\/span><\/strong><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8868\u8fbe\u91cf+\u5206\u7ec4 \u5408\u5e76\uff0c\u6574\u7406<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"tidyverse\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"rstatix\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">library(tidyverse)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">library(rstatix)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6570\u636e\u7684\u6574\u5408<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">df <- mydata %>% as_tibble() %>% pivot_longer(-<span class=\"code-snippet__number\">1<\/span>,names_to = <span class=\"code-snippet__string\">\"Sample\"<\/span>,values_to = <span class=\"code-snippet__string\">\"value\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6dfb\u52a0\u5206\u7ec4\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">df <- left_join(df,group,by=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Sample\"<\/span> = <span class=\"code-snippet__string\">\"Sample\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5233918128654971\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_Mp3qxZBs48nS2icvJ7t2lTSpFUhFcA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVf1VJJZOUxwO2pKGibibNzWNEYaJBpzg7v5TD6VPachHKI86OMp3qxZBs48nS2icvJ7t2lTSpFUhFcA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"684\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"color: rgb(249, 110, 87);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u7ed8\u5236\u706b\u5c71\u56fe<\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u91cc\u628a\u6d4b\u8bd5\u7684\u6837\u672c\u653e\u5728\u9644\u4ef6\u4e2d\u5566\uff0c\u4f9b\u53c2\u8003<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dataset <- read.table(file = <span class=\"code-snippet__string\">\".\/dataset_volcano.txt\"<\/span>, header = <span class=\"code-snippet__literal\">TRUE<\/span>, sep = <span class=\"code-snippet__string\">\"\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8bbe\u7f6ep_value\u548clogFC\u7684\u9608\u503c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cut_off_pvalue = <span class=\"code-snippet__number\">0.0000001<\/span> <span class=\"code-snippet__comment\">#\u7edf\u8ba1\u663e\u8457\u6027<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cut_off_logFC = <span class=\"code-snippet__number\">1.5<\/span> <span class=\"code-snippet__comment\">#\u5dee\u5f02\u500d\u6570\u503c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6839\u636e\u9608\u503c\u53c2\u6570\uff0c\u4e0a\u8c03\u57fa\u56e0\u8bbe\u7f6e\u4e3a\u2018up\u2019\uff0c\u4e0b\u8c03\u57fa\u56e0\u8bbe\u7f6e\u4e3a\u2018Down\u2019\uff0c\u65e0\u5dee\u5f02\u8bbe\u7f6e\u4e3a\u2018Stable\u2019\uff0c\u5e76\u4fdd\u5b58\u5230change\u5217\u4e2d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dataset$change = ifelse(dataset$P.Value < cut_off_pvalue & <span class=\"code-snippet__built_in\">abs<\/span>(dataset$logFC) >= cut_off_logFC, <\/span><\/code><code><span class=\"code-snippet_outer\"> ifelse(dataset$logFC> cut_off_logFC ,<span class=\"code-snippet__string\">'Up'<\/span>,<span class=\"code-snippet__string\">'Down'<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__string\">'Stable'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">head(dataset)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3486682808716707\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_ukvgpJ9JsU58YhibGia1QiceeIrWjA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVf1VJJZOUxwO2pKGibibNzWNZXQjOa2QUMrPFj50RDtFgbu9nuDukvgpJ9JsU58YhibGia1QiceeIrWjA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"826\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">p <- ggplot(<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\"># \u6570\u636e\u3001\u6620\u5c04\u3001\u989c\u8272<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> dataset, aes(x = logFC, y = -log10(P.Value), colour=change)) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(alpha=<span class=\"code-snippet__number\">0.8<\/span>, size=<span class=\"code-snippet__number\">1.5<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_color_manual(values=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"blue\"<\/span>, <span class=\"code-snippet__string\">\"#d2dae2\"<\/span>,<span class=\"code-snippet__string\">\"red\"<\/span>))+<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\"># \u8f85\u52a9\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> geom_vline(xintercept=<span class=\"code-snippet__built_in\">c<\/span>(-<span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">1<\/span>),lty=<span class=\"code-snippet__number\">4<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_hline(yintercept = -log10(cut_off_pvalue),lty=<span class=\"code-snippet__number\">4<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.8<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\"># \u5750\u6807\u8f74<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> labs(x=<span class=\"code-snippet__string\">\"log2(fold change)\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> y=<span class=\"code-snippet__string\">\"-log10 (p-value)\"<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\"> theme_bw()+<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__comment\"># \u56fe\u4f8b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> theme(plot.title = element_text(hjust = <span class=\"code-snippet__number\">0.5<\/span>), <\/span><\/code><code><span class=\"code-snippet_outer\"> legend.position=<span class=\"code-snippet__string\">\"right\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> legend.title = element_blank())<\/span><\/code><code><span class=\"code-snippet_outer\">p<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6dfb\u52a0\u6807\u7b7e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u5c06\u9700\u8981\u6807\u8bb0\u7684\u57fa\u56e0\u653e\u7f6e\u5728label\u5217(logFC >= 5)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(ggrepel)<\/span><\/code><code><span class=\"code-snippet_outer\">dataset$label <- ifelse(dataset$P.Value > cut_off_pvalue & <span class=\"code-snippet__built_in\">abs<\/span>(dataset$logFC) >= <span class=\"code-snippet__number\">5<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__built_in\">as.character<\/span>(dataset$gene), <span class=\"code-snippet__string\">\"\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">p + geom_label_repel(data = dataset, aes(x = dataset$logFC, <\/span><\/code><code><span class=\"code-snippet_outer\"> y = -log10(dataset$P.Value), <\/span><\/code><code><span class=\"code-snippet_outer\"> label = label),<\/span><\/code><code><span class=\"code-snippet_outer\"> size = <span class=\"code-snippet__number\">3<\/span>, box.padding = unit(<span class=\"code-snippet__number\">0.5<\/span>, <span class=\"code-snippet__string\">\"lines\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> point.padding = unit(<span class=\"code-snippet__number\">0.1<\/span>, <span class=\"code-snippet__string\">\"lines\"<\/span>), <\/span><\/code><code><span class=\"code-snippet_outer\"> segment.color = <span class=\"code-snippet__string\">\"black\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> show.legend = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8851851851851852\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_qdIFF5djt2WEUUClpAj5v49Labdbfg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVf1VJJZOUxwO2pKGibibNzWNnq09icKkqorSyamy2mIibAYOWibqdIFF5djt2WEUUClpAj5v49Labdbfg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"color: rgb(249, 110, 87);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u7406\u89e3\u706b\u5c71\u56fe<\/strong><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u706b\u5c71\u56fe\u662f\u6563\u70b9\u56fe\u7684\u4e00\u79cd\uff0c\u5b83\u5c06\u7edf\u8ba1\u6d4b\u8bd5\u4e2d\u7684\u7edf\u8ba1\u663e\u8457\u6027\u91cf\u5ea6\uff08\u5982p value\uff09\u548c\u53d8\u5316\u5e45\u5ea6\u76f8\u7ed3\u5408\uff0c\u4ece\u800c\u80fd\u591f\u5e2e\u52a9\u5feb\u901f\u76f4\u89c2\u5730\u8bc6\u522b\u90a3\u4e9b\u53d8\u5316\u5e45\u5ea6\u8f83\u5927\u4e14\u5177\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\u7684\u6570\u636e\u70b9\uff08\u57fa\u56e0\u7b49\uff09\u3002\u5e38\u5e94\u7528\u4e8e\u8f6c\u5f55\u7ec4\u7814\u7a76\uff0c\u4e5f\u80fd\u5e94\u7528\u4e8e\u57fa\u56e0\u7ec4\uff0c\u86cb\u767d\u8d28\u7ec4\uff0c\u4ee3\u8c22\u7ec4\u7b49\u7edf\u8ba1\u6570\u636e\u3002\u5173\u6ce8\u706b\u5c71\u56fe\uff0c\u5148\u7406\u89e3\u6bcf\u4e2a\u70b9\u662f\u4ec0\u4e48\uff08\u70b9\u4ee3\u8868\u57fa\u56e0\u3001\u6837\u54c1\u3001\u901a\u8def\u6216\u5176\u5b83\u7684\uff0c\u8fd9\u4e2a\u8ba4\u8bc6\u53ef\u4ee5\u6765\u81ea\u4e8e\u5e38\u8bc6\uff0c\u66f4\u51c6\u786e\u7684\u662f\u770b\u4f5c\u8005\u7684\u63cf\u8ff0\uff09\uff0c\u7136\u540e\u770b\u6a2a\u8f74\u4ee3\u8868\u4ec0\u4e48\u3001\u7eb5\u8f74\u4ee3\u8868\u4ec0\u4e48\uff0c\u518d\u770b\u56fe\u4f8b\u4e2d\u5c55\u793a\u7684\u5176\u4ed6\u4fe1\u606f\uff0c\u5982\u989c\u8272\u3001\u5927\u5c0f\u548c\u5f62\u72b6\u5206\u522b\u4ee3\u8868\u4ec0\u4e48\u3002\u8fd9\u4e9b\u90fd\u7406\u987a\u4e86\uff0c\u56fe\u7406\u89e3\u5c31\u4e0d\u96be\u4e86\u3002<\/p>\n<p style=\"white-space: normal;\">\u4f8b\u5982\u6211\u4eec\u505a\u51fa\u7684\u56fe\u4e2d\uff0c<\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<ul class=\"list-paddingleft-1\" style=\"padding-left: 40px;list-style-position: outside;\">\n<li>\n<p style=\"text-indent: 0em;\">\u6bcf\u4e2a\u70b9\u4ee3\u8868\u4e00\u4e2a\u68c0\u6d4b\u5230\u7684\u57fa\u56e0\u3002<\/p>\n<\/li>\n<li>\n<p>\u6a2a\u8f74\u548c\u7eb5\u8f74\u7528\u4e8e\u56fa\u5b9a\u70b9\u5728\u7a7a\u95f4\u7684\u4f4d\u7f6e\u3002<\/p>\n<\/li>\n<li>\n<p>\u6a2a\u8f74\u662fLog2(fold change)\uff0c\u70b9\u8d8a\u504f\u79bb\u4e2d\u5fc3\uff0c\u8868\u793a\u5dee\u5f02\u500d\u6570\u8d8a\u5927\u3002<\/p>\n<\/li>\n<li>\n<p>\u7eb5\u8f74\u662f-Log 10 (adjusted P-value)\uff0c\u70b9\u8d8a\u9760\u56fe\u7684\u9876\u90e8\u8868\u793a\u5dee\u5f02\u8d8a\u663e\u8457\u3002<\/p>\n<\/li>\n<li>\n<p>\u70b9\u7684\u5927\u5c0f\u548c\u989c\u8272\u4e5f\u53ef\u4ee5\u8868\u793a\u66f4\u591a\u7684\u5c5e\u6027\uff0c\u989c\u8272\u6807\u8bb0\u5176\u5bf9\u5e94\u7684\u57fa\u56e0\u662f\u4e0a\u8c03, \u4e0b\u8c03\u8fd8\u662f\u65e0\u5dee\u5f02\u3002<\/p>\n<\/li>\n<\/ul>\n<p style=\"white-space: normal;\">\u8fd9\u6837\u6211\u4eec\u7684\u5dee\u5f02\u5206\u6790\u56fe\u5c31\u505a\u597d\u4e86\uff0c\u5982\u679c\u6709\u95ee\u9898\u53ef\u4ee5\u4e0e\u548c\u5c0f\u7f16\u8fdb\u884c\u8ba8\u8bba\u54e6\uff5e<\/p>\n<\/section>\n<\/section>\n<section style=\"letter-spacing: 0.578px;white-space: normal;font-size: 16px;margin-bottom: 24px;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;\">\n<section style=\"margin-right: 10px;display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;height: auto;align-self: center;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"background-color: rgb(249, 172, 172);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"margin-right: 10px;padding-right: 8px;padding-left: 8px;display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);\">\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;color: rgb(249, 172, 172);\">\n<p>E<\/p>\n<\/section>\n<\/section>\n<section style=\"margin-right: 10px;padding-right: 8px;padding-left: 8px;display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);\">\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;color: rgb(249, 172, 172);\">\n<p>N<\/p>\n<\/section>\n<\/section>\n<section style=\"margin-right: 10px;padding-right: 8px;padding-left: 8px;display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;border-style: solid;border-width: 1px;border-color: rgb(249, 172, 172);\">\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;color: rgb(249, 172, 172);\">\n<p>D<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 100 100 0%;height: auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"background-color: rgb(249, 172, 172);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"letter-spacing: 0.578px;white-space: normal;text-align: center;margin-bottom: 24px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_icWAmgGMVEzREAJmM59ov44ziaMCGg-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/oCSOtP61aEWjRWAeR9wicY9JyrK13cNBaR2PPYHOsBTrS4rJOUOnJCEIxt1icWAmgGMVEzREAJmM59ov44ziaMCGg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" data-recalc-dims=\"1\"><\/p>\n<p style=\"margin-bottom: 24px;\"><br \/><\/p>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5dee\u5f02\u5206\u6790\u2014\u2014\u4ece\u539f\u7406\u5230\u706b\u5c71\u56fe\u7684\u5b9e\u73b0 \u5c0f\u5e08\u59b9 \u751f\u4fe1\u679c 2023-06- […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57336"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=57336"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57336\/revisions"}],"predecessor-version":[{"id":57343,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57336\/revisions\/57343"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=57336"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=57336"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=57336"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}