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powered-by=\"xiumi.us\">\n<p>10\u00d7genomics\u56e0\u5176\u5206\u9009\u7ec6\u80de\u6548\u679c\u597d\uff0c\u901a\u91cf\u9ad8\u7b49\u4f18\u70b9\u5df2\u6210\u4e3a\u4e3b\u6d41\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f0\u3002<\/p>\n<p>\u5927\u6d77\u54e5\u4e0a\u671f\u5bf9\u5b83\u7684\u539f\u7406\u8fdb\u884c\u4e86\u4ecb\u7ecd\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u53bb\u770b\u3002\u8fd9\u671f\u5927\u6d77\u54e5\u5c06\u4ecb\u7ecd10\u00d7genomics\u5355\u7ec6\u80de\u6d4b\u5e8f\u5206\u6790\u73af\u5883\u7684\u642d\u5efa\u4e0e\u752810\u00d7\u5e73\u53f0\u5b98\u65b9\u51fa\u7684\u6570\u636e\u5904\u7406\u6d41\u7a0bCell Ranger\u8fdb\u884c\u6570\u636e\u5904\u7406\u4e0ecount\u8868\u8fbe\u77e9\u9635\u7684\u751f\u6210\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6148148148148148\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_6kA8VMxkoDHCuQJjQNUmicRNZIQAWQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYDZj5FPKeH2PKNfTUYag0WNQmC6kA8VMxkoDHCuQJjQNUmicRNZIQAWQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section 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rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"color: rgb(255, 255, 254);line-height: 2;padding-right: 15px;padding-left: 15px;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e00\u3001\u51c6\u5907\u5de5\u4f5c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><strong>\uff08\u4e00\uff09\u8f6f\u4ef6\u5b89\u88c5<\/strong><\/p>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>1.CellRanger<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.21826809015421114\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_atvr4YVbbAicMPqTLOox7KQ29ru1aw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYnKvy34uEJAJvPMLhreXGtCx0iciatvr4YVbbAicMPqTLOox7KQ29ru1aw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"843\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>Cell Ranger\u662f10\u00d7genomics\u5b98\u65b9\u51fa\u7684\u6570\u636e\u5904\u7406\u5206\u6790\u5de5\u5177\uff0c\u5b83\u7684\u9002\u914d\u5ea6\u597d\uff0c\u529f\u80fd\u5f3a\u5927\uff0c\u64cd\u4f5c\u7b80\u5355\u7b49\u4f18\u70b9\uff0c\u6210\u4e3a\u4e86\u5355\u7ec6\u80de\u6d4b\u5e8f\u6570\u636e\u5206\u6790\u7684\u5fc5\u5907\u5de5\u5177\u3002<\/p>\n<p>\u5728\u83b7\u5f97\u5355\u7ec6\u80de\u7684\u6d4b\u5e8f\u6570\u636e\u540e\uff0c\u4f7f\u7528Cell Ranger\u6765\u8fdb\u884c\u6570\u636e\u7684\u5904\u7406\u4e0e\u5206\u6790\u7684\u5de5\u4f5c\u6d41\u7a0b\u3002<\/p>\n<p>What is Cell Ranger? -Software -Single Cell Gene Expression -Official 10x Genomics Support<\/p>\n<p>\u4e0a\u9762\u662f10\u00d7genomics\u6d4b\u5e8f\u5e73\u53f0Cell Ranger\u4e3b\u9875\u94fe\u63a5\uff0c\u8be6\u7ec6\u4ecb\u7ecd\u4e86Cell Ranger\u662f\u4ec0\u4e48\u4e0e\u600e\u4e48\u7528\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7666666666666667\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_9GRQ1Sp4r0icexQmwa0KZvlejDuiag.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYhx1832Yq4YtXic3tNQ9ygabOHria9GRQ1Sp4r0icexQmwa0KZvlejDuiag\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u4e0b\u8f7d\u9700\u8981\u586b\u5199\u4fe1\u606f\u5b8c\u6210\u6ce8\u518c\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.47327502429543244\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_Ighov0LibTG4XukdvnicicxKqLhLXQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYXLBibAegTpQfNH5KPzCHedURl6TlIghov0LibTG4XukdvnicicxKqLhLXQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1029\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u590d\u5236\u4e0b\u8f7d\u94fe\u63a5\u5230linux\u547d\u4ee4\u884c\uff0c\u8fd0\u884c\u5e76\u4e0b\u8f7d<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5472222222222223\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_v5RyRncwUoyibwdu2DCjktkqYTNNMg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYe1JrXv5EHUVrosBqJibmiaCr6Z8v5RyRncwUoyibwdu2DCjktkqYTNNMg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>#\u968f\u540e\u89e3\u538b\u7f29\u4e0b\u8f7d\u7684\u8f6f\u4ef6\u5305<\/p>\n<p>tar -zxvf cellranger-7.1.0.tar.gz<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7166666666666667\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_sC8UeAZP4SMag3eicUr6pk8fKIe9HQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYcbfFrmQNPzh9XZF7sKRoPoibMGsC8UeAZP4SMag3eicUr6pk8fKIe9HQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u5728\u547d\u4ee4\u884c\u6572\u4e00\u4e0b\uff0cok\uff0c\u8f6f\u4ef6\u5b89\u88c5\u597d\u4e86\u3002<\/p>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>2.Loupe Browser<\/p>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">Loupe Browser\u662f\u4e00\u4e2a\u684c\u9762\u5e94\u7528\u7a0b\u5e8f\uff0c\u63d0\u4f9b\u4ea4\u4e92\u5f0f\u53ef\u89c6\u5316\u5206\u6790\u6765\u81ea\u4e0d\u540c 10x \u57fa\u56e0\u7ec4\u5b66\u89e3\u51b3\u65b9\u6848\u7684\u6570\u636e\u7684\u529f\u80fd\u3002\u5b83\u80fd\u8f7b\u677e\u67e5\u8be2 10x \u57fa\u56e0\u7ec4\u5b66\u6570\u636e\u7684\u4e0d\u540c\u89c6\u56fe\u5feb\u901f\u6df1\u5165\u4e86\u89e3\u6570\u636e\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5879629629629629\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_YqVR9xoJfXibu2bmA9rbib7QfD9wug.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYiaZ4ujhE59FjQ3JC3A8y1XsiaEGfYqVR9xoJfXibu2bmA9rbib7QfD9wug\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.4871794871794872\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_HomWHkPpSG4opg8Q1Ho5d2apvZ3caQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYNZfCFmMSeRC1TUdsWRIibbv5xHomWHkPpSG4opg8Q1Ho5d2apvZ3caQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"585\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u4e0b\u8f7d\u4ecd\u7136\u9700\u8981\u586b\u5199\u4e00\u6b21\u4fe1\u606f\u3002\u586b\u5199\u597d\u4fe1\u606f\u540e\u4fbf\u53ef\u4ee5\u4e0b\u8f7d\u3002<\/p>\n<p>\u5728windows\u7cfb\u7edf\u4e0b\u6253\u5f00\u5b89\u88c5\u5305\uff0c\u8fdb\u884c\u5b89\u88c5\u5373\u53ef\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7305555555555555\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_ZorFVrymfloibIdPqFIEQZRSicvYkg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYapqE2tE1DfynnKHn3kQcNaCVRGZorFVrymfloibIdPqFIEQZRSicvYkg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u8fd9\u662f\u5e94\u7528\u7684\u754c\u9762<\/p>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>3.bcl2fastq<\/p>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u662fillumina\u5e73\u53f0\u5185\u7f6e\u7684\u4e00\u4e2a\u8f6f\u4ef6\uff0cCell Ranger\u6ca1\u6709\u96c6\u6210\u8fd9\u4e2a\u8f6f\u4ef6\uff0c\u6211\u4eec\u9700\u8981\u81ea\u5df1\u53bb\u4e0b\u8f7d\u3002\u6709\u4e86\u8fd9\u4e2a\u8f6f\u4ef6\u4e4b\u540e\uff0cCell Ranger\u53ef\u4ee5\u5b8c\u6210\u4ecebcl\u6d4b\u5e8f\u6587\u4ef6\uff0c\u5230\u6700\u540e\u7ed3\u679c\u751f\u6210\uff0c\u4e00\u6761\u9f99\u5f0f\u5730\u5b8c\u6210\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.43796296296296294\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_5qWOUiatLqRNIRjV19T3g8WHhT48tA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYDcyx8s2Yg8ibiaoSibeiby4koPS4A5qWOUiatLqRNIRjV19T3g8WHhT48tA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"text-decoration: underline;\">bcl2fastqandbcl2fastq2Conversion Software Downloads (illumina.com.cn)<\/span><\/p>\n<p><span style=\"color: rgb(0, 0, 0);\">bcl2fastq\u7684\u7f51\u7ad9<\/span><\/p>\n<p>\u540c\u6837\u9700\u8981\u6ce8\u518c\u586b\u5199\u4fe1\u606f\uff0c\u5c31\u53ef\u4ee5\u8fdb\u4e00\u6b65\u5b89\u88c5\u4e86<br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">https:\/\/support.illumina.com.cn\/content\/dam\/illumina-support\/documents\/downloads\/software\/bcl2fastq\/bcl2fastq2-v2-<span class=\"code-snippet__number\">18<\/span>-<span class=\"code-snippet__number\">0<\/span>-<span class=\"code-snippet__number\">12<\/span>-tar.zip<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\">\u8fd9\u91cc\u8d34\u4e0a\u4e0b\u8f7d\u7684\u5730\u5740<br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">unzip bcl2fastq\/bcl2fastq2-v2-<span class=\"code-snippet__number\">18<\/span>-<span class=\"code-snippet__number\">0<\/span>-<span class=\"code-snippet__number\">12<\/span>-tar.zip<\/span><\/code><code><span class=\"code-snippet_outer\">tar -zxvf bcl2fastq\/bcl2fastq2-v2-<span class=\"code-snippet__number\">18<\/span>-<span class=\"code-snippet__number\">0<\/span>-<span class=\"code-snippet__number\">12<\/span>-tar.gz<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\">#\u7f16\u8bd1\u8f6f\u4ef6<br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">cd bcl2fastq2<\/span><\/code><code><span class=\"code-snippet_outer\">chmod ugo+x src\/configure <\/span><\/code><code><span class=\"code-snippet_outer\">chmod ugo+x src\/cmake\/bootstrap\/installCmake.sh<\/span><\/code><code><span class=\"code-snippet_outer\">mkdir bin<\/span><\/code><code><span class=\"code-snippet_outer\">cd bin<\/span><\/code><code><span class=\"code-snippet_outer\">..\/src\/configure --prefix=${PWD}\/bin<\/span><\/code><code><span class=\"code-snippet_outer\">make<\/span><\/code><code><span class=\"code-snippet_outer\">make install<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>4.\u521b\u5efaconda\u73af\u5883\u5b89\u88c5\u8f6f\u4ef6<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5b89\u88c5mamba<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">conda install -y mamba<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u521b\u5efa\u865a\u62df\u73af\u5883<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">conda crate -n -y singlecell<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6fc0\u6d3b\u73af\u5883<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">conda activate singlecell<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e0b\u8f7d\u8f6f\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y fastqc<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -u multiqc<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y fastp<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y star<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y bioconductor-singler<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y samtools<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y featurecounts<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y r-seurat<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y umi_tools<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"color: rgb(255, 255, 254);line-height: 2;padding-right: 15px;padding-left: 15px;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e8c\u3001\u6570\u636e\u4e0b\u8f7d<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>1.\u53c2\u8003\u5e8f\u5217\u4e0b\u8f7d<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4eba\u57fa\u56e0\u7ec4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/refdata-gex-GRCh38-<span class=\"code-snippet__number\">2020<\/span>-A.tar.gz<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c0f\u9f20\u57fa\u56e0\u7ec4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/refdata-gex-mm10-<span class=\"code-snippet__number\">2020<\/span>-A.tar.gz<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4eba\u548c\u5c0f\u9f20\u6df7\u5408<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/refdata-gex-GRCh38-and-mm10-<span class=\"code-snippet__number\">2020<\/span>-A.tar.gz<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#Sample Index Set Sequences<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/chromium-shared-sample-indexes-plate.csv<\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/chromium-single-cell-sample-indexes-plate-v1.csv<\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/gemcode-single-cell-sample-indexes-plate.csv<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>2.\u7ec3\u4e60\u6570\u636e\u4e0b\u8f7d<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#pbmc 1k\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/samples\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/<span class=\"code-snippet__number\">3.0<\/span>.0\/pbmc_1k_v3\/pbmc_1k_v3_fastqs.tar<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__number\">1000<\/span>\u4e2a\u5355\u7ec6\u80de\u7684\u6d4b\u5e8f\u6570\u636e<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"color: rgb(255, 255, 254);line-height: 2;padding-right: 15px;padding-left: 15px;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e09\u3001\u4f7f\u7528cellranger\u5206\u6790\u5355\u7ec6\u80de\u6570\u636e<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>\uff08\u4e00\uff09Basecalling<\/p>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">1.\u5c06\u6d4b\u5e8f\u539f\u59cb\u6570\u636ebcl\u6587\u4ef6\u8f6c\u6362\u4e3afastq\u6587\u4ef6<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6848\u4f8b\u6570\u636e\uff0cilluminca\u6d4b\u5e8f\u539f\u59cb\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/cellranger-tiny-bcl-<span class=\"code-snippet__number\">1.2<\/span>.0.tar.gz<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/cellranger-tiny-bcl-simple-<span class=\"code-snippet__number\">1.2<\/span>.0.csv<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">tar -zxvf cellranger-tiny-bcl-<span class=\"code-snippet__number\">1.2<\/span>.0.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3914174252275683\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_VhdyPyxTro88lQmFibibaCxVaTVZrA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYCOoL8XIDIyynFQ33w8gicVlPMB2VhdyPyxTro88lQmFibibaCxVaTVZrA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"769\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u8fd9\u662f\u8fd9\u4e2a\u539f\u59cb\u6d4b\u5e8f\u6570\u636e\u6240\u5305\u542b\u7684\u5185\u5bb9\uff0c\u6570\u636e\u5728\u8fd9\u4e2aData\u76ee\u5f55\u4e0b\u9762\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.14040114613180515\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_cbZkvJTSOGsk6xJu58VCPHCUjOl8ag.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYBGPaPVp9fic0mLrfuPtnvwCMGcbZkvJTSOGsk6xJu58VCPHCUjOl8ag\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"698\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.33425925925925926\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/13_QMibhAwCmlgkqNMibyS5uia73N03uQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYB0xicqpQILsgicSDa1NSruHRuyxXjQMibhAwCmlgkqNMibyS5uia73N03uQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u975e\u5e38\u7b80\u5355\uff0c\u53ea\u8981\u8f93\u5165\u6587\u4ef6\uff0c\u53ca\u8f93\u51fa\u7684\u6587\u4ef6\u5939\u5c31\u884c\u4e86\u3002\u56e0\u4e3a\u6570\u636e\u5f88\u5c0f\uff0c\u5f88\u5feb\u5c31\u8fd0\u884c\u597d\u4e86\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.2612137203166227\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/14_qCtaKZqE7vdZJYZM1ibpibCFN5tQtw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyY3soLJYlibGH0YYBCnjztXaLvpmLqCtaKZqE7vdZJYZM1ibpibCFN5tQtw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"379\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u751f\u6210\u7684fastq\u5c31\u5728\u8fd9\u4e2a\u6587\u4ef6\u5939\u91cc\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.34991119005328597\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/15_VffQOiaeSYCzGVxlibenehbIjVoKuA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYt7D9wOfD4ssIG8ZXjIsLicxdP48VffQOiaeSYCzGVxlibenehbIjVoKuA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"563\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">Test_sample\u5c31\u662f\u6700\u7ec8\u7684\u7ed3\u679c\u3002\u6587\u4ef6\u540d\u4f1a\u6839\u636esimple\u6587\u4ef6\u7684\u540d\u79f0\u547d\u540d\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.37905604719764013\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/16_YicJaZibg9SXvrAAvxPOUiaXmrCezA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYyZgwTzCbapHj5OGibkQEwyaczsibJYicJaZibg9SXvrAAvxPOUiaXmrCezA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"678\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u6253\u5f00\u7b2c\u4e00\u4e2a\u770b\u4e00\u4e0b\uff0c\u5176\u4e3aIndex\u5e8f\u5217\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.365296803652968\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/17_Ch0flRVADDeUGhVCSuzr1T2bf152YA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyY5lKB6PDpziaH68kQdtBJcMC8cCh0flRVADDeUGhVCSuzr1T2bf152YA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"657\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">Read1\u4e3abarcod+UMI<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.20277777777777778\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/18_bW8GDks0g2d1NeHKuPAJFrCO0ZueVA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYARian1UxEPPIuiaS3CgxaeCMnAibW8GDks0g2d1NeHKuPAJFrCO0ZueVA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>Read2\u662f\u771f\u6b63\u7684\u6211\u4eec\u60f3\u8981\u7684\u6d4b\u5e8f\u5e8f\u5217\u3002<\/p>\n<p>\u8be5\u90e8\u5206\u4e3a\u6f14\u793a\u5982\u4f55\u5c06bcl\u8f6c\u5316\u4e3afastq\u3002<br \/><\/p>\n<p>2.\u8d28\u63a7<br \/><\/p>\n<\/section>\n<p powered-by=\"xiumi.us\">\u63a5\u4e0b\u6765\u6211\u4eec\u5bf9\u7ec3\u4e60\u6570\u636e\u8fdb\u884c\u5904\u7406\uff0c\u8fd9\u91cc\u6211\u4eec\u6253\u5f00\u6848\u4f8b\u7684\u6570\u636e\u3002\u4f7f\u7528\u901a\u914d\u7b26*\u5339\u914d\u6240\u6709fastq\u7684read2\u3002\u7136\u540e\u8fdb\u884c\u8d28\u63a7\u3002<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9read2 \u8fdb\u884c\u8d28\u63a7<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ll pbmc_1k_v3_fastqs\/pbmc_1k_v3*_R2_001.fastq.gz<\/span><\/code><code><span class=\"code-snippet_outer\">fastqc -t <span class=\"code-snippet__number\">12<\/span> -f fastq -o qc1 pbmc_1k_v3_fastqs\/pbmc_1k_v3*_R2_001.fastq.gz<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.30914368650217705\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/19_bC9icQibFV1kmUDR0mux6TUuJUNTtA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyY2GIe913EibLYT2MpuqUhGEb4l5UbC9icQibFV1kmUDR0mux6TUuJUNTtA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"689\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5212962962962963\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/20_EpvQp5JucssEthFHbSBibXcOWBILEw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYlbgFtuO2m4HcCeibVicyGws7axeEpvQp5JucssEthFHbSBibXcOWBILEw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u8d28\u63a7\u7ed3\u679c\uff0c\u663e\u793a\u6d4b\u5e8f\u6570\u636e\u8d28\u91cf\u8f83\u597d\u3002<\/p>\n<p>10\u00d7genomics\u7684\u6d4b\u5e8f\u6570\u636e\u901a\u5e38\u8d28\u91cf\u8f83\u597d\uff0c\u6240\u4ee5\u7edd\u5927\u90e8\u5206\u4e0d\u7528\u8fdb\u884c\u8d28\u63a7\u4ee5\u53ca\u8fc7\u6ee4\u3002<\/p>\n<p>\u5b9e\u5728\u8fc7\u6ee4\u7684\u8bdd\u5462\uff0c\u7531\u4e8eread1\u548cread2\u4e0d\u4e00\u6837\uff0c\u524d\u8005\u662fbarcode\uff0c\u540e\u8005\u662f\u6d4b\u5e8f\u6570\u636e\uff0c\u5bf9\u6d4b\u5e8f\u6570\u636e\u8fc7\u6ee4\u6389\u4ee5\u540e\uff0c\u4e8c\u8005\u7d22\u5f15\u4e0d\u5339\u914d\uff0c\u5f15\u8d77\u64cd\u4f5c\u9ebb\u70e6\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>\uff08\u4e8c\uff09\u751f\u6210\u8868\u8fbe\u77e9\u9635<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4212962962962963\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/21_2FOPZiaoEMmViaXAAyDl9wvia4iajA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYJeD30totmrk8hKlTTuKjTLevx63H2FOPZiaoEMmViaXAAyDl9wvia4iajA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>Cellranger count\u7684\u9009\u9879\u53c2\u6570\uff0c\u8be5\u8f6f\u4ef6\u4e3b\u6253\u7684\u5c31\u662f\u4e00\u4e2a\u7b80\u5355\u65b9\u4fbf\uff0c\u7528\u8d77\u6765\u7b80\u5355\u7edd\u5bf9\u662f\u4e2a\u4f18\u70b9\u3002<\/p>\n<p>\u8f93\u5165\u524d\u9762\u4e09\u4e2a\u53c2\u6570\u5c31\u53ef\u4ee5\u5de5\u4f5c\u4e86\u3002<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8ba1\u7b97count \u77e9\u9635\uff0cpbmc 1000\u4e2a\u7ec6\u80de\uff0c\u7b49\u53f7\u4e4b\u95f4\u4e0d\u8981\u6709\u7a7a\u683c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cellranger count --id=run_count_1kpbmcs --fastqs=pbmc_1k_v3_fastqs --sample=pbmc_1k_v3 <\/span><\/code><code><span class=\"code-snippet_outer\"> --transcriptome=refdata-gex-GRCh38-<span class=\"code-snippet__number\">2020<\/span>-A\/ <\/span><\/code><code><span class=\"code-snippet_outer\"> --localcores=<span class=\"code-snippet__number\">12<\/span> --localmem=<span class=\"code-snippet__number\">32<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5592592592592592\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/22_Z4FsxUlCVTwpzOEiaDVC4icZ44AWyQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYktlcbRGgiaSLGLAgMfs0L8oP88WZ4FsxUlCVTwpzOEiaDVC4icZ44AWyQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u8fd9\u6837\u5b83\u5c31\u8fd0\u884c\u8d77\u6765\u4e86\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9975550122249389\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/23_10BeTjE4b80EKibEFQPFV7mrPNvvsg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYh2l3dJ2rEmv2O8VXYp1orgNmB10BeTjE4b80EKibEFQPFV7mrPNvvsg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"409\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u6700\u7ec8\u7684\u7ed3\u679c\u5728outs\u91cc\u9762<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7468982630272953\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/24_3nxhb0Uzxoc9I33wTHwAjUoog42OTQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYe1mmuO4q9Znmygn7A1OChlG43nxhb0Uzxoc9I33wTHwAjUoog42OTQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"403\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"box-shadow: rgb(170, 170, 170) 0px 0px 10px inset;border-color: rgb(192, 200, 209);padding: 10px;border-width: 1px;border-style: solid;background-color: rgb(250, 250, 239);\">\n<section style=\"color: rgb(51, 51, 51);text-align: center;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: justify;text-wrap: wrap;\">\u5927\u6d77\u54e5\u4eca\u5929\u7684\u8bb2\u89e3\u5c31\u5230\u8fd9\u91cc\u4e86~\u4e0b\u671f\u6211\u5c06\u4f1a\u5e26\u6765\u5bf9\u5355\u7ec6\u80de\u6d4b\u5e8f\u5206\u6790\u7ed3\u679c\u7684\u89e3\u8bfb~\u6211\u4eec\u4e0d\u89c1\u4e0d\u6563<\/p>\n<p style=\"text-align: justify;text-wrap: wrap;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-left: 10px;padding-right: 10px;\">\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-left: 10px;padding-right: 10px;\">\n<section style=\"transform: scale(2);transform-origin: center center;margin-top: 50px;margin-bottom: 50px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-left: -6px;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 215, 173) rgb(69, 107, 176) rgb(69, 107, 176);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-left: -6px;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 215, 173) rgb(69, 107, 176) rgb(69, 107, 176);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-left: -6px;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 215, 173) rgb(69, 107, 176) rgb(69, 107, 176);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 38%;align-self: center;flex: 0 0 auto;height: auto;padding: 3px 23px;\">\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 100%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.025925925925926\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/25_NFyts1jxLwtHpMHTxskBcGCRyBZibw.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/YHGRXkSxjST4qLG4uyZGiat2dSF4icr7CDJopBdYOsYbuPCJl1vOEz4OR6DNFyts1jxLwtHpMHTxskBcGCRyBZibw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;transform: rotateY(180deg);\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-left: -6px;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 215, 173) rgb(69, 107, 176) rgb(69, 107, 176);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-left: -6px;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 215, 173) rgb(69, 107, 176) rgb(69, 107, 176);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-left: -6px;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 215, 173) rgb(69, 107, 176) rgb(69, 107, 176);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(231, 215, 173);font-size: 18px;\">\n<p><strong>\u751f\u4fe1\u6ee9\u516c\u4f17\u53f7<\/strong><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<\/section>\n<section style=\"border-bottom: 2px solid rgb(0, 150, 136);text-align: left;font-size: 12px;\" powered-by=\"xiumi.us\">\n<p><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOYJyjlsib6bQ74Wibtria9gSC4sqocafb5YBQcZWdQRrtP2Xo7vaFWaecA\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" 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rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;border-width: 0px;margin-left: 4px;\">\n<section style=\"text-align: center;margin-top: -4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 9px;height: 9px;vertical-align: top;overflow: hidden;border-width: 2px;border-radius: 47px;border-style: solid;border-color: rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;\">\n<section style=\"margin-right: 0%;margin-left: 0%;text-align: center;transform: translate3d(-1px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 11px;padding-left: 11px;line-height: 1;text-align: justify;\">\n<p style=\"text-align: left;text-wrap: wrap;\"><strong>\u70b9\u51fb\u84dd\u5b57 \u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;line-height: 0;flex: 0 0 0%;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(59, 149, 115);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(59, 149, 115);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>10\u00d7genomics\u56e0\u5176\u5206\u9009\u7ec6\u80de\u6548\u679c\u597d\uff0c\u901a\u91cf\u9ad8\u7b49\u4f18\u70b9\u5df2\u6210\u4e3a\u4e3b\u6d41\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u5e73\u53f0\u3002<\/p>\n<p>\u5927\u6d77\u54e5\u4e0a\u671f\u5bf9\u5b83\u7684\u539f\u7406\u8fdb\u884c\u4e86\u4ecb\u7ecd\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u53bb\u770b\u3002\u8fd9\u671f\u5927\u6d77\u54e5\u5c06\u4ecb\u7ecd10\u00d7genomics\u5355\u7ec6\u80de\u6d4b\u5e8f\u5206\u6790\u73af\u5883\u7684\u642d\u5efa\u4e0e\u752810\u00d7\u5e73\u53f0\u5b98\u65b9\u51fa\u7684\u6570\u636e\u5904\u7406\u6d41\u7a0bCell Ranger\u8fdb\u884c\u6570\u636e\u5904\u7406\u4e0ecount\u8868\u8fbe\u77e9\u9635\u7684\u751f\u6210\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6148148148148148\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_6kA8VMxkoDHCuQJjQNUmicRNZIQAWQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYDZj5FPKeH2PKNfTUYag0WNQmC6kA8VMxkoDHCuQJjQNUmicRNZIQAWQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u901a\u5e38\u8fd9\u662f\u5355\u7ec6\u80de\u5206\u6790\u7684\u6574\u4e2a\u6d41\u7a0b\u3002<\/p>\n<p>\u4e0e\u8bb8\u591a\u7ec4\u5b66\u5206\u6790\u4e00\u6837\uff0c\u5148\u5c06FASTQ\u6587\u4ef6\u6bd4\u5bf9\uff0c\u8f6c\u6362\u4e3a\u4e8c\u8fdb\u5236\u7684BAM\u6587\u4ef6\uff0c\u7136\u540e\u8fdb\u884c\u57fa\u56e0\u8868\u8fbe\u8ba1\u6570\u3002\u62ff\u5230\u8868\u8fbe\u77e9\u9635\u540e\u624d\u80fd\u8fdb\u884c\u4e0b\u4e00\u6b65\u7684\u5206\u6790\u3002<\/p>\n<p>\u4eca\u5929\u5927\u6d77\u54e5\u5e26\u5927\u5bb6\u5b8c\u6210\u6d41\u7a0b\u7684\u642d\u5efa\u4e0e\u4e0a\u6e38\u7684\u7684\u6570\u636e\u5904\u7406\u3002\u5c31\u662f\u8fd9\u5f20\u56fe\u4e0a\u9762\u4e09\u4e2a\u6b65\u9aa4\u3002<\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"color: rgb(255, 255, 254);line-height: 2;padding-right: 15px;padding-left: 15px;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e00\u3001\u51c6\u5907\u5de5\u4f5c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><strong>\uff08\u4e00\uff09\u8f6f\u4ef6\u5b89\u88c5<\/strong><\/p>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>1.CellRanger<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.21826809015421114\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_atvr4YVbbAicMPqTLOox7KQ29ru1aw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYnKvy34uEJAJvPMLhreXGtCx0iciatvr4YVbbAicMPqTLOox7KQ29ru1aw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"843\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>Cell Ranger\u662f10\u00d7genomics\u5b98\u65b9\u51fa\u7684\u6570\u636e\u5904\u7406\u5206\u6790\u5de5\u5177\uff0c\u5b83\u7684\u9002\u914d\u5ea6\u597d\uff0c\u529f\u80fd\u5f3a\u5927\uff0c\u64cd\u4f5c\u7b80\u5355\u7b49\u4f18\u70b9\uff0c\u6210\u4e3a\u4e86\u5355\u7ec6\u80de\u6d4b\u5e8f\u6570\u636e\u5206\u6790\u7684\u5fc5\u5907\u5de5\u5177\u3002<\/p>\n<p>\u5728\u83b7\u5f97\u5355\u7ec6\u80de\u7684\u6d4b\u5e8f\u6570\u636e\u540e\uff0c\u4f7f\u7528Cell Ranger\u6765\u8fdb\u884c\u6570\u636e\u7684\u5904\u7406\u4e0e\u5206\u6790\u7684\u5de5\u4f5c\u6d41\u7a0b\u3002<\/p>\n<p>What is Cell Ranger? -Software -Single Cell Gene Expression -Official 10x Genomics Support<\/p>\n<p>\u4e0a\u9762\u662f10\u00d7genomics\u6d4b\u5e8f\u5e73\u53f0Cell Ranger\u4e3b\u9875\u94fe\u63a5\uff0c\u8be6\u7ec6\u4ecb\u7ecd\u4e86Cell Ranger\u662f\u4ec0\u4e48\u4e0e\u600e\u4e48\u7528\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7666666666666667\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_9GRQ1Sp4r0icexQmwa0KZvlejDuiag.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYhx1832Yq4YtXic3tNQ9ygabOHria9GRQ1Sp4r0icexQmwa0KZvlejDuiag\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u4e0b\u8f7d\u9700\u8981\u586b\u5199\u4fe1\u606f\u5b8c\u6210\u6ce8\u518c\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.47327502429543244\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_Ighov0LibTG4XukdvnicicxKqLhLXQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYXLBibAegTpQfNH5KPzCHedURl6TlIghov0LibTG4XukdvnicicxKqLhLXQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1029\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u590d\u5236\u4e0b\u8f7d\u94fe\u63a5\u5230linux\u547d\u4ee4\u884c\uff0c\u8fd0\u884c\u5e76\u4e0b\u8f7d<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5472222222222223\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_v5RyRncwUoyibwdu2DCjktkqYTNNMg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYe1JrXv5EHUVrosBqJibmiaCr6Z8v5RyRncwUoyibwdu2DCjktkqYTNNMg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>#\u968f\u540e\u89e3\u538b\u7f29\u4e0b\u8f7d\u7684\u8f6f\u4ef6\u5305<\/p>\n<p>tar -zxvf cellranger-7.1.0.tar.gz<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7166666666666667\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_sC8UeAZP4SMag3eicUr6pk8fKIe9HQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYcbfFrmQNPzh9XZF7sKRoPoibMGsC8UeAZP4SMag3eicUr6pk8fKIe9HQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u5728\u547d\u4ee4\u884c\u6572\u4e00\u4e0b\uff0cok\uff0c\u8f6f\u4ef6\u5b89\u88c5\u597d\u4e86\u3002<\/p>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>2.Loupe Browser<\/p>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">Loupe Browser\u662f\u4e00\u4e2a\u684c\u9762\u5e94\u7528\u7a0b\u5e8f\uff0c\u63d0\u4f9b\u4ea4\u4e92\u5f0f\u53ef\u89c6\u5316\u5206\u6790\u6765\u81ea\u4e0d\u540c 10x \u57fa\u56e0\u7ec4\u5b66\u89e3\u51b3\u65b9\u6848\u7684\u6570\u636e\u7684\u529f\u80fd\u3002\u5b83\u80fd\u8f7b\u677e\u67e5\u8be2 10x \u57fa\u56e0\u7ec4\u5b66\u6570\u636e\u7684\u4e0d\u540c\u89c6\u56fe\u5feb\u901f\u6df1\u5165\u4e86\u89e3\u6570\u636e\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5879629629629629\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_YqVR9xoJfXibu2bmA9rbib7QfD9wug.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYiaZ4ujhE59FjQ3JC3A8y1XsiaEGfYqVR9xoJfXibu2bmA9rbib7QfD9wug\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.4871794871794872\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_HomWHkPpSG4opg8Q1Ho5d2apvZ3caQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYNZfCFmMSeRC1TUdsWRIibbv5xHomWHkPpSG4opg8Q1Ho5d2apvZ3caQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"585\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u4e0b\u8f7d\u4ecd\u7136\u9700\u8981\u586b\u5199\u4e00\u6b21\u4fe1\u606f\u3002\u586b\u5199\u597d\u4fe1\u606f\u540e\u4fbf\u53ef\u4ee5\u4e0b\u8f7d\u3002<\/p>\n<p>\u5728windows\u7cfb\u7edf\u4e0b\u6253\u5f00\u5b89\u88c5\u5305\uff0c\u8fdb\u884c\u5b89\u88c5\u5373\u53ef\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7305555555555555\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_ZorFVrymfloibIdPqFIEQZRSicvYkg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYapqE2tE1DfynnKHn3kQcNaCVRGZorFVrymfloibIdPqFIEQZRSicvYkg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u8fd9\u662f\u5e94\u7528\u7684\u754c\u9762<\/p>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>3.bcl2fastq<\/p>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u662fillumina\u5e73\u53f0\u5185\u7f6e\u7684\u4e00\u4e2a\u8f6f\u4ef6\uff0cCell Ranger\u6ca1\u6709\u96c6\u6210\u8fd9\u4e2a\u8f6f\u4ef6\uff0c\u6211\u4eec\u9700\u8981\u81ea\u5df1\u53bb\u4e0b\u8f7d\u3002\u6709\u4e86\u8fd9\u4e2a\u8f6f\u4ef6\u4e4b\u540e\uff0cCell Ranger\u53ef\u4ee5\u5b8c\u6210\u4ecebcl\u6d4b\u5e8f\u6587\u4ef6\uff0c\u5230\u6700\u540e\u7ed3\u679c\u751f\u6210\uff0c\u4e00\u6761\u9f99\u5f0f\u5730\u5b8c\u6210\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.43796296296296294\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_5qWOUiatLqRNIRjV19T3g8WHhT48tA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYDcyx8s2Yg8ibiaoSibeiby4koPS4A5qWOUiatLqRNIRjV19T3g8WHhT48tA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"text-decoration: underline;\">bcl2fastqandbcl2fastq2Conversion Software Downloads (illumina.com.cn)<\/span><\/p>\n<p><span style=\"color: rgb(0, 0, 0);\">bcl2fastq\u7684\u7f51\u7ad9<\/span><\/p>\n<p>\u540c\u6837\u9700\u8981\u6ce8\u518c\u586b\u5199\u4fe1\u606f\uff0c\u5c31\u53ef\u4ee5\u8fdb\u4e00\u6b65\u5b89\u88c5\u4e86<br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">https:\/\/support.illumina.com.cn\/content\/dam\/illumina-support\/documents\/downloads\/software\/bcl2fastq\/bcl2fastq2-v2-<span class=\"code-snippet__number\">18<\/span>-<span class=\"code-snippet__number\">0<\/span>-<span class=\"code-snippet__number\">12<\/span>-tar.zip<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\">\u8fd9\u91cc\u8d34\u4e0a\u4e0b\u8f7d\u7684\u5730\u5740<br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">unzip bcl2fastq\/bcl2fastq2-v2-<span class=\"code-snippet__number\">18<\/span>-<span class=\"code-snippet__number\">0<\/span>-<span class=\"code-snippet__number\">12<\/span>-tar.zip<\/span><\/code><code><span class=\"code-snippet_outer\">tar -zxvf bcl2fastq\/bcl2fastq2-v2-<span class=\"code-snippet__number\">18<\/span>-<span class=\"code-snippet__number\">0<\/span>-<span class=\"code-snippet__number\">12<\/span>-tar.gz<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\">#\u7f16\u8bd1\u8f6f\u4ef6<br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">cd bcl2fastq2<\/span><\/code><code><span class=\"code-snippet_outer\">chmod ugo+x src\/configure <\/span><\/code><code><span class=\"code-snippet_outer\">chmod ugo+x src\/cmake\/bootstrap\/installCmake.sh<\/span><\/code><code><span class=\"code-snippet_outer\">mkdir bin<\/span><\/code><code><span class=\"code-snippet_outer\">cd bin<\/span><\/code><code><span class=\"code-snippet_outer\">..\/src\/configure --prefix=${PWD}\/bin<\/span><\/code><code><span class=\"code-snippet_outer\">make<\/span><\/code><code><span class=\"code-snippet_outer\">make install<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>4.\u521b\u5efaconda\u73af\u5883\u5b89\u88c5\u8f6f\u4ef6<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5b89\u88c5mamba<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">conda install -y mamba<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u521b\u5efa\u865a\u62df\u73af\u5883<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">conda crate -n -y singlecell<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6fc0\u6d3b\u73af\u5883<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">conda activate singlecell<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e0b\u8f7d\u8f6f\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y fastqc<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -u multiqc<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y fastp<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y star<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y bioconductor-singler<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y samtools<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y featurecounts<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y r-seurat<\/span><\/code><code><span class=\"code-snippet_outer\">mamba install -y umi_tools<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"color: rgb(255, 255, 254);line-height: 2;padding-right: 15px;padding-left: 15px;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e8c\u3001\u6570\u636e\u4e0b\u8f7d<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>1.\u53c2\u8003\u5e8f\u5217\u4e0b\u8f7d<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4eba\u57fa\u56e0\u7ec4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/refdata-gex-GRCh38-<span class=\"code-snippet__number\">2020<\/span>-A.tar.gz<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c0f\u9f20\u57fa\u56e0\u7ec4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/refdata-gex-mm10-<span class=\"code-snippet__number\">2020<\/span>-A.tar.gz<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4eba\u548c\u5c0f\u9f20\u6df7\u5408<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/refdata-gex-GRCh38-and-mm10-<span class=\"code-snippet__number\">2020<\/span>-A.tar.gz<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#Sample Index Set Sequences<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/chromium-shared-sample-indexes-plate.csv<\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/chromium-single-cell-sample-indexes-plate-v1.csv<\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/gemcode-single-cell-sample-indexes-plate.csv<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>2.\u7ec3\u4e60\u6570\u636e\u4e0b\u8f7d<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#pbmc 1k\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/samples\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/<span class=\"code-snippet__number\">3.0<\/span>.0\/pbmc_1k_v3\/pbmc_1k_v3_fastqs.tar<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__number\">1000<\/span>\u4e2a\u5355\u7ec6\u80de\u7684\u6d4b\u5e8f\u6570\u636e<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin: 20px 0%;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(204, 223, 237);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);padding-right: 10px;align-self: flex-start;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;min-width: 10%;flex: 0 0 auto;height: auto;background-color: rgb(60, 86, 125);border-width: 0px;border-radius: 0px 50px 50px 0px;border-style: none;border-color: rgb(62, 62, 62);align-self: flex-start;\">\n<section style=\"color: rgb(255, 255, 254);line-height: 2;padding-right: 15px;padding-left: 15px;text-align: justify;\" powered-by=\"xiumi.us\">\n<p><strong>\u4e09\u3001\u4f7f\u7528cellranger\u5206\u6790\u5355\u7ec6\u80de\u6570\u636e<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>\uff08\u4e00\uff09Basecalling<\/p>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">1.\u5c06\u6d4b\u5e8f\u539f\u59cb\u6570\u636ebcl\u6587\u4ef6\u8f6c\u6362\u4e3afastq\u6587\u4ef6<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6848\u4f8b\u6570\u636e\uff0cilluminca\u6d4b\u5e8f\u539f\u59cb\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/cellranger-tiny-bcl-<span class=\"code-snippet__number\">1.2<\/span>.0.tar.gz<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">wget https:\/\/cf.10xgenomics.com\/supp\/cell-<span class=\"code-snippet__built_in\">exp<\/span>\/cellranger-tiny-bcl-simple-<span class=\"code-snippet__number\">1.2<\/span>.0.csv<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">tar -zxvf cellranger-tiny-bcl-<span class=\"code-snippet__number\">1.2<\/span>.0.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3914174252275683\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_VhdyPyxTro88lQmFibibaCxVaTVZrA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYCOoL8XIDIyynFQ33w8gicVlPMB2VhdyPyxTro88lQmFibibaCxVaTVZrA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"769\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u8fd9\u662f\u8fd9\u4e2a\u539f\u59cb\u6d4b\u5e8f\u6570\u636e\u6240\u5305\u542b\u7684\u5185\u5bb9\uff0c\u6570\u636e\u5728\u8fd9\u4e2aData\u76ee\u5f55\u4e0b\u9762\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.14040114613180515\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_cbZkvJTSOGsk6xJu58VCPHCUjOl8ag.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYBGPaPVp9fic0mLrfuPtnvwCMGcbZkvJTSOGsk6xJu58VCPHCUjOl8ag\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"698\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.33425925925925926\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/13_QMibhAwCmlgkqNMibyS5uia73N03uQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYB0xicqpQILsgicSDa1NSruHRuyxXjQMibhAwCmlgkqNMibyS5uia73N03uQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u975e\u5e38\u7b80\u5355\uff0c\u53ea\u8981\u8f93\u5165\u6587\u4ef6\uff0c\u53ca\u8f93\u51fa\u7684\u6587\u4ef6\u5939\u5c31\u884c\u4e86\u3002\u56e0\u4e3a\u6570\u636e\u5f88\u5c0f\uff0c\u5f88\u5feb\u5c31\u8fd0\u884c\u597d\u4e86\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.2612137203166227\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/14_qCtaKZqE7vdZJYZM1ibpibCFN5tQtw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyY3soLJYlibGH0YYBCnjztXaLvpmLqCtaKZqE7vdZJYZM1ibpibCFN5tQtw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"379\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u751f\u6210\u7684fastq\u5c31\u5728\u8fd9\u4e2a\u6587\u4ef6\u5939\u91cc\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.34991119005328597\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/15_VffQOiaeSYCzGVxlibenehbIjVoKuA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYt7D9wOfD4ssIG8ZXjIsLicxdP48VffQOiaeSYCzGVxlibenehbIjVoKuA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"563\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">Test_sample\u5c31\u662f\u6700\u7ec8\u7684\u7ed3\u679c\u3002\u6587\u4ef6\u540d\u4f1a\u6839\u636esimple\u6587\u4ef6\u7684\u540d\u79f0\u547d\u540d\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.37905604719764013\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/16_YicJaZibg9SXvrAAvxPOUiaXmrCezA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYyZgwTzCbapHj5OGibkQEwyaczsibJYicJaZibg9SXvrAAvxPOUiaXmrCezA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"678\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u6253\u5f00\u7b2c\u4e00\u4e2a\u770b\u4e00\u4e0b\uff0c\u5176\u4e3aIndex\u5e8f\u5217\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.365296803652968\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/17_Ch0flRVADDeUGhVCSuzr1T2bf152YA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyY5lKB6PDpziaH68kQdtBJcMC8cCh0flRVADDeUGhVCSuzr1T2bf152YA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"657\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">Read1\u4e3abarcod+UMI<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.20277777777777778\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/18_bW8GDks0g2d1NeHKuPAJFrCO0ZueVA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYARian1UxEPPIuiaS3CgxaeCMnAibW8GDks0g2d1NeHKuPAJFrCO0ZueVA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>Read2\u662f\u771f\u6b63\u7684\u6211\u4eec\u60f3\u8981\u7684\u6d4b\u5e8f\u5e8f\u5217\u3002<\/p>\n<p>\u8be5\u90e8\u5206\u4e3a\u6f14\u793a\u5982\u4f55\u5c06bcl\u8f6c\u5316\u4e3afastq\u3002<br \/><\/p>\n<p>2.\u8d28\u63a7<br \/><\/p>\n<\/section>\n<p powered-by=\"xiumi.us\">\u63a5\u4e0b\u6765\u6211\u4eec\u5bf9\u7ec3\u4e60\u6570\u636e\u8fdb\u884c\u5904\u7406\uff0c\u8fd9\u91cc\u6211\u4eec\u6253\u5f00\u6848\u4f8b\u7684\u6570\u636e\u3002\u4f7f\u7528\u901a\u914d\u7b26*\u5339\u914d\u6240\u6709fastq\u7684read2\u3002\u7136\u540e\u8fdb\u884c\u8d28\u63a7\u3002<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9read2 \u8fdb\u884c\u8d28\u63a7<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ll pbmc_1k_v3_fastqs\/pbmc_1k_v3*_R2_001.fastq.gz<\/span><\/code><code><span class=\"code-snippet_outer\">fastqc -t <span class=\"code-snippet__number\">12<\/span> -f fastq -o qc1 pbmc_1k_v3_fastqs\/pbmc_1k_v3*_R2_001.fastq.gz<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.30914368650217705\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/19_bC9icQibFV1kmUDR0mux6TUuJUNTtA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyY2GIe913EibLYT2MpuqUhGEb4l5UbC9icQibFV1kmUDR0mux6TUuJUNTtA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"689\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5212962962962963\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/20_EpvQp5JucssEthFHbSBibXcOWBILEw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYlbgFtuO2m4HcCeibVicyGws7axeEpvQp5JucssEthFHbSBibXcOWBILEw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u8d28\u63a7\u7ed3\u679c\uff0c\u663e\u793a\u6d4b\u5e8f\u6570\u636e\u8d28\u91cf\u8f83\u597d\u3002<\/p>\n<p>10\u00d7genomics\u7684\u6d4b\u5e8f\u6570\u636e\u901a\u5e38\u8d28\u91cf\u8f83\u597d\uff0c\u6240\u4ee5\u7edd\u5927\u90e8\u5206\u4e0d\u7528\u8fdb\u884c\u8d28\u63a7\u4ee5\u53ca\u8fc7\u6ee4\u3002<\/p>\n<p>\u5b9e\u5728\u8fc7\u6ee4\u7684\u8bdd\u5462\uff0c\u7531\u4e8eread1\u548cread2\u4e0d\u4e00\u6837\uff0c\u524d\u8005\u662fbarcode\uff0c\u540e\u8005\u662f\u6d4b\u5e8f\u6570\u636e\uff0c\u5bf9\u6d4b\u5e8f\u6570\u636e\u8fc7\u6ee4\u6389\u4ee5\u540e\uff0c\u4e8c\u8005\u7d22\u5f15\u4e0d\u5339\u914d\uff0c\u5f15\u8d77\u64cd\u4f5c\u9ebb\u70e6\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;margin-top: 20px;margin-bottom: 10px;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(255, 238, 211);min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"color: rgb(32, 70, 126);text-align: center;letter-spacing: 1.2px;padding-right: 20px;padding-left: 20px;\" powered-by=\"xiumi.us\">\n<p>\uff08\u4e8c\uff09\u751f\u6210\u8868\u8fbe\u77e9\u9635<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4212962962962963\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/21_2FOPZiaoEMmViaXAAyDl9wvia4iajA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYJeD30totmrk8hKlTTuKjTLevx63H2FOPZiaoEMmViaXAAyDl9wvia4iajA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>Cellranger count\u7684\u9009\u9879\u53c2\u6570\uff0c\u8be5\u8f6f\u4ef6\u4e3b\u6253\u7684\u5c31\u662f\u4e00\u4e2a\u7b80\u5355\u65b9\u4fbf\uff0c\u7528\u8d77\u6765\u7b80\u5355\u7edd\u5bf9\u662f\u4e2a\u4f18\u70b9\u3002<\/p>\n<p>\u8f93\u5165\u524d\u9762\u4e09\u4e2a\u53c2\u6570\u5c31\u53ef\u4ee5\u5de5\u4f5c\u4e86\u3002<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8ba1\u7b97count \u77e9\u9635\uff0cpbmc 1000\u4e2a\u7ec6\u80de\uff0c\u7b49\u53f7\u4e4b\u95f4\u4e0d\u8981\u6709\u7a7a\u683c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cellranger count --id=run_count_1kpbmcs --fastqs=pbmc_1k_v3_fastqs --sample=pbmc_1k_v3 <\/span><\/code><code><span class=\"code-snippet_outer\"> --transcriptome=refdata-gex-GRCh38-<span class=\"code-snippet__number\">2020<\/span>-A\/ <\/span><\/code><code><span class=\"code-snippet_outer\"> --localcores=<span class=\"code-snippet__number\">12<\/span> --localmem=<span class=\"code-snippet__number\">32<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5592592592592592\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/22_Z4FsxUlCVTwpzOEiaDVC4icZ44AWyQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYktlcbRGgiaSLGLAgMfs0L8oP88WZ4FsxUlCVTwpzOEiaDVC4icZ44AWyQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u8fd9\u6837\u5b83\u5c31\u8fd0\u884c\u8d77\u6765\u4e86\u3002<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9975550122249389\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/23_10BeTjE4b80EKibEFQPFV7mrPNvvsg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYh2l3dJ2rEmv2O8VXYp1orgNmB10BeTjE4b80EKibEFQPFV7mrPNvvsg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"409\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\">\u6700\u7ec8\u7684\u7ed3\u679c\u5728outs\u91cc\u9762<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7468982630272953\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/24_3nxhb0Uzxoc9I33wTHwAjUoog42OTQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSNSNSA4ZJsgLwqzl7vCHyYe1mmuO4q9Znmygn7A1OChlG43nxhb0Uzxoc9I33wTHwAjUoog42OTQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"403\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"box-shadow: rgb(170, 170, 170) 0px 0px 10px inset;border-color: rgb(192, 200, 209);padding: 10px;border-width: 1px;border-style: solid;background-color: rgb(250, 250, 239);\">\n<section style=\"color: rgb(51, 51, 51);text-align: center;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p style=\"text-align: justify;text-wrap: wrap;\">\u5927\u6d77\u54e5\u4eca\u5929\u7684\u8bb2\u89e3\u5c31\u5230\u8fd9\u91cc\u4e86~\u4e0b\u671f\u6211\u5c06\u4f1a\u5e26\u6765\u5bf9\u5355\u7ec6\u80de\u6d4b\u5e8f\u5206\u6790\u7ed3\u679c\u7684\u89e3\u8bfb~\u6211\u4eec\u4e0d\u89c1\u4e0d\u6563<\/p>\n<p style=\"text-align: justify;text-wrap: wrap;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-left: 10px;padding-right: 10px;\">\n<section style=\"font-size: 14px;letter-spacing: 2px;line-height: 1.8;padding-left: 10px;padding-right: 10px;\">\n<section style=\"transform: scale(2);transform-origin: center center;margin-top: 50px;margin-bottom: 50px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-left: -6px;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 3px 3px 0px 0px;border-color: rgb(231, 215, 173) rgb(231, 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