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style=\"mso-spacerun:'yes';font-family:\u5fae\u8f6f\u96c5\u9ed1;font-size:10.5000pt;mso-font-kerning:1.0000pt;\">3\u2019<span style=\"font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\u7aef\u6d4b\u5e8f\u548c\u5168\u957f\u6d4b\u5e8f\u5177\u6709\u76f8\u4f3c\u7684\u5efa\u5e93\u6d41\u7a0b\u548c\u5206\u6790\u6b65\u9aa4\uff0c\u56e0\u6b64\u4e0b\u9762\u5c0f\u679c\u4ee53<\/span>\u2019\u7aef\u6d4b\u5e8f\u4e3a\u4f8b\u8ddf\u5927\u5bb6\u4e00\u8d77\u5b66\u4e60\u5355\u7ec6\u80de\u6d4b\u5e8f\u7684\u5efa\u5e93\u6d41\u7a0b\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u5728\u5355\u7ec6\u80de\u6d4b\u5e8f\u4e2d\uff0c\u51dd\u80f6\u5fae\u73e0\u662f\u5176\u6838\u5fc3\u7ec4\u6210\u90e8\u5206\u4e4b\u4e00\u3002\u51dd\u80f6\u5fae\u73e0\u7ed3\u6784\u4e3b\u8981\u5305\u62ecTruSeq Read 1\uff0c10\u00d7 barcode\uff0cUMI\u4ee5\u53caPoly(dT)\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.29411764705882354\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_ick6Wc496ygxf1sVDBy6pDmia2GPAg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAySluCsI4g499lVRLKEdY2sFj2HIPlmhOIDuYRSbk6vXRGiatJick6Wc496ygxf1sVDBy6pDmia2GPAg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"782\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5fae\u8f6f\u96c5\u9ed1;font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5fae\u8f6f\u96c5\u9ed1;font-size:10.5000pt;mso-font-kerning:1.0000pt;\">TruSeq Read 1\u662f\u6d4b\u5e8f\u5f15\u7269\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5fae\u8f6f\u96c5\u9ed1;font-size:10.5000pt;mso-font-kerning:1.0000pt;\">10xbarcode\u662f\u4e00\u6bb5\u542b\u670916\u4e2a\u78b1\u57fa\u7684\u5e8f\u5217\uff0c\u4e0d\u540c\u5fae\u73e0\u672a\u5b9a\u4e0d\u540cbarcode\uff0c\u53ef\u4ee5\u533a\u5206\u4e0d\u540c\u7684\u7ec6\u80de\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5fae\u8f6f\u96c5\u9ed1;font-size:10.5000pt;mso-font-kerning:1.0000pt;\">UMI\u662f\u4e00\u6bb5\u542b\u670912\u4e2a\u78b1\u57fa\u7684\u5e8f\u5217\uff0c\u5728cDNA\u8fdb\u884cPCR\u6269\u589e\u524d\u5f15\u5165\u7684\u5e8f\u5217\uff0c\u53ef\u4ee5\u786e\u5b9acDNA\u6269\u589e\u8fc7\u7a0b\u4ea7\u751f\u7684\u62f7\u8d1d\u6570\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5fae\u8f6f\u96c5\u9ed1;font-size:10.5000pt;mso-font-kerning:1.0000pt;\">Poly(dT)\u5e8f\u5217\u4e0emRNA\u7684PolyA\u5c3e\u5df4\u7ed3\u5408\uff0c\u9006\u8f6c\u51facDNA\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5fae\u8f6f\u96c5\u9ed1;font-size:10.5000pt;mso-font-kerning:1.0000pt;\">\u78c1\u73e0\u901a\u8fc7\u5fae\u6d41\u63a7\u7cfb\u7edf\u4e0e\u7ec6\u80de\u6df7\u5408\uff0c\u5f62\u6210\u6cb9\u5305\u6c34\u6db2\u6ef4\uff0c\u5177\u4f53\u6b65\u9aa4\u5982\u4e0b\u56fe\u6240\u793a\uff0c<o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5042735042735043\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_zQo1X7OR1p6dW1ZUBgDDHZUkiczfVQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAySluCsI4g499lVRLKEdY2sQ0gX4W1vQHDCOQxD7u5xfUia31zQo1X7OR1p6dW1ZUBgDDHZUkiczfVQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"702\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5fae\u8f6f\u96c5\u9ed1;font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><span style=\"font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\u5728\u4e0a\u8ff0\u6b65\u9aa4\u4e2d\u5f62\u6210\u7684\u6db2\u6ef4\u4e2d\uff0c\u78c1\u73e0\u4e0a\u7684DNA\u5e8f\u5217\u4e0emRNA\u4e92\u8865\u914d\u5bf9\uff0c\u5728\u9176\u7684\u4f5c\u7528\u4e0b\u53d1\u751f\u9006\u8f6c\u5f55\u3002<\/span><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4601941747572815\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_Tg0B1zmoOH6ZoGwucYYhVYuf6xHdLw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAySluCsI4g499lVRLKEdY2sHLohwKEJsP1Iz2iau5m0Iy02gTg0B1zmoOH6ZoGwucYYhVYuf6xHdLw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"515\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u968f\u540e\u5c06\u6db2\u6ef4\u7834\u788e\u83b7\u5f97\u5355\u94fecDNA\uff0c\u4ee5cDNA\u4e3a\u6a21\u677f\u8fdb\u884c\u6269\u589e\u3001\u8d28\u68c0\u3001\u5efa\u5e93\u7b49\u64cd\u4f5c\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.31746031746031744\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_maYJEqMLROAdGh6ic2PsJ5IGWyJkpw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAySluCsI4g499lVRLKEdY2speSpZQxibiaTibRNu7rj1sJCS6lhmaYJEqMLROAdGh6ic2PsJ5IGWyJkpw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"font-size: 14px;font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u7531\u4e8e\u4e8c\u4ee3\u6d4b\u5e8f\u7684\u8bfb\u957f\u9650\u5236\uff0c\u5c06\u8d28\u68c0\u5408\u683c\u540e\u7684cDNA\u901a\u8fc7\u8d85\u58f0\u6253\u788e\u6210\u4e8c\u4e09\u767ebp\u7684\u7247\u6bb5\uff0c\u6dfb\u52a0P5\u3001P7\u3001sample<\/span> index\u3001i5\u3001i7\u5e8f\u5217\uff0c\u5b8c\u6210\u5efa\u5e93\u64cd\u4f5c\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u4ee5\u4e0a\u5c31\u662f\u5355\u7ec6\u80de\u6d4b\u5e8f\u5efa\u5e93\u6d41\u7a0b\u4ee5\u53ca\u57fa\u672c\u7684\u539f\u7406\u3002\u5173\u4e8e\u5355\u7ec6\u80de\u6d4b\u5e8f\u66f4\u8be6\u7ec6\u7684\u539f\u7406\u53ef\u4ee5\u53c2\u8003\u4e0b\u9762\u52a0\u5dde\u5927\u5b66\u7684\u89c6\u9891\u3002\u6b22\u8fce\u5927\u5bb6\u7ee7\u7eed\u5173\u6ce8\u5c0f\u679c\uff0c\u5c0f\u679c\u5c06\u7ee7\u7eed\u4e3a\u5927\u5bb6\u5e26\u6765\u5355\u7ec6\u80de\u6d4b\u5e8f\u7684\u5206\u6790\u6d41\u7a0b\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u611f\u5174\u8da3\u5c0f\u4f19\u4f34\u53ef\u4ee5\u70b9\u51fb\u89c6\u9891\u94fe\u63a5\u770b\u5b8c\u6574\u8bfe\u7a0b\u54e6\uff1a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;color: rgb(5, 99, 193);text-decoration: underline;font-size: 14px;\">https:\/\/www.bilibili.com\/video\/BV1q34y1m7FC\/spm_id_from=333.788.recommend_more_video.-1<\/span><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;font-size: 14px;\">\u3002<\/span><\/p>\n<\/section>\n<section style=\"font-size: 16px;\">\n<section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;\">\u5c0f\u679c\u4eca\u5929\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\uff0c\u6b22\u8fce\u5927\u5bb6\u548c\u5c0f\u679c\u4e00\u8d77\u8ba8\u8bba\u5b66\u4e60\u54e6\uff01\u6211\u4eec\u4e0b\u671f\u518d\u89c1~<\/span><span style=\"text-align: center;letter-spacing: 0.034em;\"><\/span><\/p>\n<p><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;padding-right: 15px;padding-left: 15px;\">\n<section style=\"height: auto;transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;transform: translate3d(19px, 0px, 0px) rotateX(180deg);margin-top: 10px;margin-bottom: -3px;\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(255, 255, 255);\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 230px;background-color: rgb(217, 217, 217);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(255, 255, 255);\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 230px;background-color: rgb(217, 217, 217);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(255, 255, 255);\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 230px;background-color: rgb(217, 217, 217);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;border-style: solid;border-width: 1px;border-color: rgb(217, 217, 217);padding: 14px 10px;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 15px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 0px 12px;border-left-color: rgb(212, 32, 2);padding-left: 9px;\">\n<section style=\"text-align: justify;font-size: 17px;color: rgb(62, 62, 62);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><strong>\u5c0f\u679c\u53cb\u60c5\u63a8\u8350<\/strong><\/p>\n<\/section>\n<section style=\"margin-top: 3px;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(217, 217, 217);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"text-align: justify;font-size: 11px;color: rgb(62, 62, 62);\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u597d\u7528\u53c8\u514d\u8d39\u7684\u5de5\u5177\u5b89\u5229<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.35777777777777775\" 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