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15px;\">R\u8bed\u8a00\u5728\u751f\u7269\u4fe1\u606f\u5b66\u548c\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u662f\u4e00\u79cd\u5e38\u7528\u7684\u7f16\u7a0b\u8bed\u8a00\uff0c\u5b83\u63d0\u4f9b\u4e86\u4e30\u5bcc\u7684\u5de5\u5177\u548c\u5e93\u6765\u5904\u7406\u548c\u5206\u6790\u751f\u7269\u6570\u636e\u3002\u5176\u4e2d\uff0c\u4e00\u4e2a\u91cd\u8981\u7684R\u5305\u662fscater\uff0c\u5b83\u662f\u4e00\u4e2a\u4e13\u4e3a\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\uff08scRNA-seq\uff09\u6570\u636e\u5206\u6790\u800c\u8bbe\u8ba1\u7684\u5305\uff0c\u4e3a\u7814\u7a76\u4eba\u5458\u5728\u5904\u7406\u3001\u53ef\u89c6\u5316\u548c\u7406\u89e3\u5355\u7ec6\u80de\u6570\u636e\u65b9\u9762\u63d0\u4f9b\u4e86\u5f3a\u5927\u7684\u652f\u6301\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" 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mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\"><strong>scRNA-seq\u6280\u672f<\/strong><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">scRNA-seq\u6280\u672f\u5df2\u7ecf\u6210\u4e3a\u4e86\u7814\u7a76\u5355\u4e2a\u7ec6\u80de\u57fa\u56e0\u8868\u8fbe\u7684\u4e3b\u8981\u624b\u6bb5\u3002\u7136\u800c\uff0c\u8fd9\u79cd\u9ad8\u7ef4\u5ea6\u7684\u6570\u636e\u9700\u8981\u590d\u6742\u7684\u5904\u7406\u548c\u5206\u6790\u65b9\u6cd5\u624d\u80fd\u4ece\u4e2d\u63d0\u53d6\u6709\u7528\u7684\u751f\u7269\u5b66\u4fe1\u606f\u3002\u5728\u9762\u5bf9\u5982\u6b64\u590d\u6742\u7684\u6311\u6218\u65f6\uff0cscater\u5305\u6210\u4e3a\u4e86\u7814\u7a76\u4eba\u5458\u4e0d\u53ef\u6216\u7f3a\u7684\u4f19\u4f34\u548c\u89e3\u51b3\u65b9\u6848\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" 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460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 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left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\"><strong>\u8d28\u91cf\u63a7\u5236<\/strong><\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">\u8d28\u91cf\u63a7\u5236\u662f\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u7684\u9996\u8981\u4efb\u52a1\u4e4b\u4e00\uff0c\u800cscater\u5305\u5728\u8fd9\u65b9\u9762\u53d1\u6325\u7740\u91cd\u8981\u4f5c\u7528\u3002\u901a\u8fc7\u8be5\u5305\uff0c\u7528\u6237\u53ef\u4ee5\u8bc6\u522b\u5e76\u79fb\u9664\u4f4e\u8d28\u91cf\u7ec6\u80de\uff0c\u4ece\u800c\u51cf\u5c11\u5206\u6790\u4e2d\u7684\u566a\u58f0\u548c\u8bef\u5dee\u3002\u8fd9\u4e00\u6b65\u7684\u5173\u952e\u5728\u4e8e\u4fdd\u969c\u540e\u7eed\u5206\u6790\u7684\u53ef\u9760\u6027\u548c\u51c6\u786e\u6027\uff0c\u4f7f\u5f97\u7528\u6237\u80fd\u591f\u66f4\u52a0\u81ea\u4fe1\u5730\u89e3\u91ca\u548c\u5e94\u7528\u5206\u6790\u7ed3\u679c\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">\u5f52\u4e00\u5316\u65b9\u6cd5<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">\u9664\u4e86\u8d28\u91cf\u63a7\u5236\uff0cscater\u5305\u8fd8\u63d0\u4f9b\u4e86\u591a\u79cd\u5f52\u4e00\u5316\u65b9\u6cd5\uff0c\u5141\u8bb8\u7528\u6237\u5728\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u8fdb\u884c\u6bd4\u8f83\u3002\u8fd9\u5728\u7814\u7a76\u4e2d\u5c24\u4e3a\u91cd\u8981\uff0c\u56e0\u4e3a\u4e0d\u540c\u5b9e\u9a8c\u6279\u6b21\u548c\u6280\u672f\u5dee\u5f02\u53ef\u80fd\u4f1a\u5f15\u5165\u504f\u5dee\u3002scater\u7684\u5f52\u4e00\u5316\u65b9\u6cd5\u53ef\u4ee5\u5e2e\u52a9\u7528\u6237\u6d88\u9664\u8fd9\u4e9b\u504f\u5dee\uff0c\u4f7f\u5f97\u6570\u636e\u66f4\u5177\u53ef\u6bd4\u6027\uff0c\u4ece\u800c\u66f4\u5bb9\u6613\u6355\u6349\u5230\u751f\u7269\u5b66\u4e0a\u771f\u5b9e\u7684\u5dee\u5f02\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u8981\u4f7f\u7528scater\u5305\uff0c\u53ef\u4ee5\u5728R\u4e2d\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\u8fdb\u884c\u5b89\u88c5\u548c\u52a0\u8f7d\uff1a<\/span><\/p>\n<p><span style=\"font-size: 15px;\"><em style=\"color: rgb(178, 178, 178);letter-spacing: 0.578px;white-space: normal;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 14px;letter-spacing: 0.476px;text-decoration: underline;\">\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c111\u201d\u9886\u53d6\u4ee3\u7801\uff0c\u4ee3\u7801\u7f16\u53f7\uff1a231008<\/span><\/span><\/em><\/span><\/p>\n<\/section>\n<section style=\"display: inline-block;width: 100%;vertical-align: top;overflow-x: auto;\" powered-by=\"xiumi.us\">\n<section style=\"overflow-y: hidden;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">> install.packages(<span class=\"code-snippet__string\">\"scater\"<\/span>) #\u5b89\u88c5scater\u8bed\u8a00\u5305<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> library(scater) #\u52a0\u8f7d\u8bed\u8a00\u5305<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">\u5728\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u5206\u6790\u4e2d\uff0c\u6570\u636e\u8d28\u91cf\u63a7\u5236\u548c\u9884\u5904\u7406\u662f\u786e\u4fdd\u51c6\u786e\u53ef\u9760\u5206\u6790\u7684\u57fa\u7840\u6b65\u9aa4\u3002<\/span><span style=\"font-size: 15px;color: rgb(255, 169, 0);\"><strong>scater\u4f5c\u4e3a\u4e00\u6b3e\u5f3a\u5927\u7684\u5206\u6790\u5de5\u5177\uff0c\u4e3a\u7528\u6237\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u9ad8\u6548\u7684\u529f\u80fd\u6765\u5904\u7406\u8fd9\u4e9b\u5173\u952e\u4efb\u52a1\u3002<\/strong><\/span><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">\u9996\u5148\uff0c\u6570\u636e\u8d28\u91cf\u63a7\u5236\u65b9\u9762\uff0cscater\u5141\u8bb8\u7528\u6237\u901a\u8fc7\u8ba1\u7b97\u591a\u79cd\u8d28\u91cf\u6307\u6807\uff0c\u5982\u7ec6\u80de\u7684\u6d4b\u5e8f\u6df1\u5ea6\u3001\u57fa\u56e0\u8868\u8fbe\u4e30\u5ea6\u548c\u7ec6\u80de\u7684dropout\u7387\uff0c\u6765\u8bc4\u4f30\u6570\u636e\u7684\u8d28\u91cf\u3002\u8fd9\u4e9b\u6307\u6807\u53ef\u4ee5\u5e2e\u52a9\u7528\u6237\u8bc6\u522b\u51fa\u4f4e\u8d28\u91cf\u7684\u7ec6\u80de\u548c\u57fa\u56e0\uff0c\u4ece\u800c\u4fdd\u7559\u9ad8\u8d28\u91cf\u7684\u6570\u636e\u7528\u4e8e\u540e\u7eed\u5206\u6790\u3002\u6b64\u5916\uff0cscater\u8fd8\u53ef\u4ee5\u751f\u6210\u8be6\u7ec6\u7684\u8d28\u91cf\u63a7\u5236\u62a5\u544a\uff0c\u5e2e\u52a9\u7528\u6237\u5168\u9762\u4e86\u89e3\u6570\u636e\u4e2d\u7684\u95ee\u9898\u548c\u8d8b\u52bf\u3002\u901a\u8fc7\u8bc6\u522b\u548c\u5254\u9664\u6280\u672f\u566a\u58f0\u548c\u6279\u6b21\u6548\u5e94\uff0cscater\u786e\u4fdd\u4e86\u540e\u7eed\u5206\u6790\u7684\u53ef\u9760\u6027\u548c\u51c6\u786e\u6027\u3002<\/span><\/p>\n<p><strong><span style=\"font-size: 15px;color: rgb(255, 169, 0);\">\u7ec6\u80de\u805a\u7c7b\u548c\u964d\u7ef4\u5206\u6790\u662f\u5355\u7ec6\u80de\u6570\u636e\u89e3\u6790\u7684\u5173\u952e\u6b65\u9aa4\uff0c\u6709\u52a9\u4e8e\u63ed\u793a\u6570\u636e\u4e2d\u7684\u7ec6\u80de\u7c7b\u578b\u548c\u7ed3\u6784\u3002<\/span><\/strong><span style=\"font-size: 15px;\">\u4e0e\u6b64\u7c7b\u4f3c\uff0cscater\u63d0\u4f9b\u4e86\u591a\u79cd\u805a\u7c7b\u7b97\u6cd5\u548c\u964d\u7ef4\u65b9\u6cd5\uff0c\u5982\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u3001t\u5206\u5e03\u968f\u673a\u90bb\u57df\u5d4c\u5165\uff08t-SNE\uff09\u548cUMAP\uff08Uniform Manifold Approximation and Projection\uff09\u3002\u8fd9\u4e9b\u65b9\u6cd5\u53ef\u4ee5\u5c06\u9ad8\u7ef4\u6570\u636e\u6620\u5c04\u5230\u66f4\u4f4e\u7ef4\u5ea6\u7684\u7a7a\u95f4\uff0c\u4f7f\u7528\u6237\u80fd\u591f\u5728\u4e8c\u7ef4\u6216\u4e09\u7ef4\u56fe\u4e2d\u89c2\u5bdf\u6570\u636e\u7684\u5206\u5e03\uff0c\u66f4\u597d\u5730\u7406\u89e3\u4e0d\u540c\u7ec6\u80de\u4e4b\u95f4\u7684\u76f8\u4f3c\u6027\u548c\u5dee\u5f02\u6027\u3002\u8fd9\u79cd\u7ec6\u80de\u805a\u7c7b\u548c\u964d\u7ef4\u5206\u6790\u4e3a\u6570\u636e\u7684\u53ef\u89c6\u5316\u63d0\u4f9b\u4e86\u6709\u529b\u652f\u6301\uff0c\u6709\u52a9\u4e8e\u7814\u7a76\u4eba\u5458\u66f4\u6df1\u5165\u5730\u63a2\u7d22\u7ec6\u80de\u7fa4\u7684\u7ed3\u6784\u548c\u7279\u5f81\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u662f\u4e86\u89e3\u4e0d\u540c\u6761\u4ef6\u4e0b\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u7684\u5173\u952e\u6b65\u9aa4\uff0c\u6709\u52a9\u4e8e\u8bc6\u522b\u5728\u7279\u5b9a\u751f\u7269\u5b66\u8fc7\u7a0b\u4e2d\u8d77\u5173\u952e\u4f5c\u7528\u7684\u57fa\u56e0\u3002<\/span><strong><span style=\"font-size: 15px;color: rgb(255, 169, 0);\">scater\u63d0\u4f9b\u4e86\u4e30\u5bcc\u7684\u7edf\u8ba1\u65b9\u6cd5\u548c\u53ef\u89c6\u5316\u5de5\u5177\uff0c\u4f7f\u7528\u6237\u80fd\u591f\u6bd4\u8f83\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u3001\u6761\u4ef6\u6216\u65f6\u95f4\u70b9\u4e4b\u95f4\u7684\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u5dee\u5f02<\/span><\/strong><span style=\"font-size: 15px;\">\u3002\u901a\u8fc7\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u6df1\u5165\u4e86\u89e3\u5728\u4e0d\u540c\u7ec6\u80de\u7fa4\u4e4b\u95f4\u7684\u751f\u7269\u5b66\u5dee\u5f02\uff0c\u4e3a\u8fdb\u4e00\u6b65\u7684\u673a\u5236\u89e3\u6790\u63d0\u4f9b\u7ebf\u7d22\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u5bf9\u4e8e\u7ec6\u80de\u53d1\u80b2\u8f68\u8ff9\u5206\u6790\uff0cscater\u662f\u4e00\u6b3e\u5f3a\u5927\u7684\u5de5\u5177\uff0c\u5b83\u80fd\u591f\u5e2e\u52a9\u7528\u6237\u63ed\u793a\u7ec6\u80de\u5206\u5316\u548c\u53d1\u80b2\u7684\u52a8\u6001\u53d8\u5316\u8fc7\u7a0b\u3002\u901a\u8fc7\u5efa\u7acb\u7ec6\u80de\u72b6\u6001\u7684\u53d1\u5c55\u8f68\u8ff9\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u66f4\u597d\u5730\u7406\u89e3\u7ec6\u80de\u72b6\u6001\u4e4b\u95f4\u7684\u5173\u7cfb\uff0c\u63a2\u7d22\u7ec6\u80de\u5206\u5316\u548c\u6f14\u5316\u7684\u8f68\u8ff9\uff0c\u4ece\u800c\u6df1\u5165\u4e86\u89e3\u53d1\u80b2\u8fc7\u7a0b\u4e2d\u7684\u91cd\u8981\u6027\u8d28\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">scater\u7684\u6570\u636e\u53ef\u89c6\u5316\u529f\u80fd\u4e3a\u7814\u7a76\u4eba\u5458\u5448\u73b0\u5206\u6790\u7ed3\u679c\u63d0\u4f9b\u4e86\u6709\u529b\u7684\u652f\u6301\u3002<span style=\"color: rgb(255, 169, 0);\"><strong>\u901a\u8fc7\u5185\u7f6e\u7684\u591a\u79cd\u6570\u636e\u53ef\u89c6\u5316\u5de5\u5177\uff0c\u5982\u6563\u70b9\u56fe\u3001\u70ed\u56fe\u548c\u8f68\u8ff9\u56fe\uff0c\u7528\u6237\u80fd\u591f\u4ee5\u76f4\u89c2\u7684\u65b9\u5f0f\u5c55\u793a\u4ed6\u4eec\u7684\u53d1\u73b0\u3002<\/strong><\/span>\u8fd9\u6837\u7684\u53ef\u89c6\u5316\u6709\u52a9\u4e8e\u66f4\u6e05\u6670\u5730\u4f20\u8fbe\u5206\u6790\u7ed3\u679c\uff0c\u5e76\u4e3a\u5176\u4ed6\u7814\u7a76\u4eba\u5458\u7406\u89e3\u5355\u7ec6\u80de\u6570\u636e\u7684\u6d1e\u5bdf\u63d0\u4f9b\u4e86\u65b9\u4fbf\u3002<\/span><\/p>\n<p><strong><span style=\"font-size: 15px;\">\u793a\u4f8b\uff1a<\/span><\/strong><span style=\"font-size: 15px;\"><\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u5f53\u4f7f\u7528scater\u5305\u65f6\uff0c\u901a\u5e38\u9700\u8981\u5bfc\u5165\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u96c6\uff0c\u5e76\u8fdb\u884c\u4e00\u7cfb\u5217\u7684\u9884\u5904\u7406\u3001\u53ef\u89c6\u5316\u548c\u5206\u6790\u6b65\u9aa4\u3002\u9996\u5148\uff0c\u5047\u8bbe\u4f60\u5df2\u7ecf\u5b89\u88c5\u4e86scater\u5305\u548c\u5176\u4ed6\u5fc5\u8981\u7684\u4f9d\u8d56\u9879\u3002\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u5c06\u52a0\u8f7d\u793a\u4f8b\u6570\u636e\u96c6\u5e76\u8fdb\u884c\u57fa\u672c\u7684\u5206\u6790\u548c\u53ef\u89c6\u5316\u3002<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"># \u52a0\u8f7d\u6240\u9700\u7684\u5e93<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> library(scater)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> library(SingleCellExperiment)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u4ecescater\u5305\u52a0\u8f7d\u793a\u4f8b\u6570\u636e\u96c6<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> data(\"sc_example_counts\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u5c06\u793a\u4f8b\u6570\u636e\u96c6\u8f6c\u6362\u4e3aSingleCellExperiment\u5bf9\u8c61<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- SingleCellExperiment(<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">+ assays = list(counts = as.matrix(sc_example_counts))<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">+ )<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u8fdb\u884c\u8d28\u91cf\u63a7\u5236<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- calculateQCMetrics(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u7ed8\u5236\u7ec6\u80de\u6570\u76ee\u548c\u57fa\u56e0\u6570\u76ee\u7684\u6563\u70b9\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> plotQC(sce, type = \"cell\", x = \"total_features_by_counts\", y = \"n_genes_by_counts\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u8fdb\u884c\u5f52\u4e00\u5316<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- normalize(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u4f7f\u7528PCA\u8fdb\u884c\u964d\u7ef4<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- runPCA(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u7ed8\u5236PCA\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> plotReducedDim(sce, dimred = \"PCA\")<\/span><\/code><\/pre>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7148014440433214\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_rKHqzp6wuMiamoFHSrVgl8N02O8Cpg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzhoNjktYmc4lN1vzqfM4E3Upw9xHdzWm9u4vmqBiaFBu2qg2rKHqzp6wuMiamoFHSrVgl8N02O8Cpg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"># \u8fdb\u884c\u805a\u7c7b<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- clusterCells(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u7ed8\u5236\u805a\u7c7b\u7ed3\u679c\u7684t-SNE\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- runTSNE(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> plotReducedDim(sce, dimred = \"TSNE\", label = \"clusters\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<section style=\"display: inline-block;width: 100%;vertical-align: top;overflow-x: auto;\" powered-by=\"xiumi.us\">\n<section style=\"overflow: hidden;width: 10%;max-width: 10% !important;\">\n<section style=\"display: inline-block;vertical-align: middle;width: 100%;\" powered-by=\"xiumi.us\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.49547920433996384\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_QQYvnB6VNO6xquMiahtFXszXrNBxUw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzhoNjktYmc4lN1vzqfM4E3BIzfe8iaOIbhBjNb3zw8wIgBKnQQYvnB6VNO6xquMiahtFXszXrNBxUw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"553\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"># \u8fdb\u884c\u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- DESeq(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u7ed8\u5236\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684MA\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> plotMA(sce, contrast = \"condition_B_vs_A\")<\/span><\/code><\/pre>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6985294117647058\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_IcTTWBQkgzbA5cUDuqFicTEESeyjzQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzhoNjktYmc4lN1vzqfM4E3qZnIh7j4tP1PhdTEmEF1XMMNcIcTTWBQkgzbA5cUDuqFicTEESeyjzQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"272\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.44223826714801445\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_dVxLRWhIEHXBkzlbFxGGGURMWlHNng.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzhoNjktYmc4lN1vzqfM4E3R6svhtGJpCtDBaONWhVPM0AGdVxLRWhIEHXBkzlbFxGGGURMWlHNng\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"># \u7ed8\u5236\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u70ed\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> plotHeatmap(sce, contrast = \"condition_B_vs_A\", cluster_rows = FALSE)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<section style=\"display: inline-block;width: 100%;vertical-align: top;overflow-x: auto;\" powered-by=\"xiumi.us\">\n<section style=\"overflow-y: hidden;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3345388788426763\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_63bKxa8F5viaHqibEtsu3BQOhnGntQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzhoNjktYmc4lN1vzqfM4E3ywJEoPyVPdM2pw8sfjoMvUIGsf63bKxa8F5viaHqibEtsu3BQOhnGntQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"553\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">\u4e0a\u8ff0\u793a\u4f8b\u4ee3\u7801\u6f14\u793a\u4e86\u5982\u4f55\u4f7f\u7528scater\u5305\u52a0\u8f7d\u793a\u4f8b\u6570\u636e\u96c6\u3001\u8fdb\u884c\u8d28\u91cf\u63a7\u5236\u3001\u5f52\u4e00\u5316\u3001\u964d\u7ef4\u3001\u805a\u7c7b\u548c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u5e76\u751f\u6210\u7ec6\u80de\u6570\u76ee\u4e0e\u57fa\u56e0\u6570\u76ee\u7684\u6563\u70b9\u56fe\u3001PCA\u56fe\u3001t-SNE\u56fe\u3001MA\u56fe\u548c\u70ed\u56fe\u3002\u8fd9\u4e9b\u53ef\u89c6\u5316\u56fe\u8868\u6709\u52a9\u4e8e\u6211\u4eec\u76f4\u89c2\u5730\u7406\u89e3\u6570\u636e\u7684\u7279\u5f81\u548c\u6a21\u5f0f\uff0c\u8fdb\u800c\u4ece\u4e2d\u6316\u6398\u751f\u7269\u5b66\u76f8\u5173\u7684\u4fe1\u606f\u3002<\/span><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">\u7ed3\u8bba<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br mpa-from-tpl=\"t\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"font-size: 15px;\">\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u5728scRNA-seq\u6570\u636e\u5206\u6790\u4e2d\u626e\u6f14\u7740\u81f3\u5173\u91cd\u8981\u7684\u89d2\u8272\uff0c\u800cscater\u5305\u4e3a\u8fd9\u4e00\u73af\u8282\u63d0\u4f9b\u4e86\u5f3a\u5927\u7684\u652f\u6301\u3002<\/span><span style=\"color: rgb(255, 169, 0);\"><strong><span style=\"font-size: 15px;letter-spacing: 0.034em;\">\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u65e8\u5728\u8bc6\u522b\u5728\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u6216\u6761\u4ef6\u4e4b\u95f4\u8868\u8fbe\u6c34\u5e73\u663e\u8457\u53d8\u5316\u7684\u57fa\u56e0\uff0c\u8fd9\u6709\u52a9\u4e8e\u6211\u4eec\u66f4\u6df1\u5165\u5730\u7406\u89e3\u7ec6\u80de\u7684\u529f\u80fd\u548c\u8c03\u63a7\u673a\u5236<\/span><\/strong><\/span><strong><span style=\"font-size: 15px;\">\u3002<\/span><\/strong><span style=\"font-size: 15px;\">scater\u5305\u901a\u8fc7\u5b9e\u73b0\u591a\u79cd\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u65b9\u6cd5\uff0c\u5982edgeR\u548cDESeq2\uff0c\u4e3a\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4e86\u591a\u6837\u5316\u7684\u5de5\u5177\uff0c\u5e2e\u52a9\u4ed6\u4eec\u4ece\u6d77\u91cf\u7684\u6570\u636e\u4e2d\u6316\u6398\u51fa\u6709\u610f\u4e49\u7684\u751f\u7269\u5b66\u4fe1\u606f\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">\u5728\u4f7f\u7528scater\u5305\u8fdb\u884c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u65f6\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u6839\u636e\u5b9e\u9645\u60c5\u51b5\u9009\u62e9\u9002\u5408\u7684\u65b9\u6cd5<\/span>\u3002<span style=\"color: rgb(255, 169, 0);\"><strong><span style=\"font-size: 15px;\">\u4f8b\u5982\uff0cedgeR\u548cDESeq2\u662f\u4e24\u79cd\u5e38\u7528\u7684\u7edf\u8ba1\u65b9\u6cd5\uff0c\u5b83\u4eec\u80fd\u591f\u8003\u8651\u5230\u6570\u636e\u7684\u79bb\u6563\u6027\u548c\u6279\u6b21\u6548\u5e94\uff0c\u4ece\u800c\u66f4\u51c6\u786e\u5730\u6355\u6349\u57fa\u56e0\u8868\u8fbe\u4e4b\u95f4\u7684\u5dee\u5f02<\/span><\/strong><\/span><strong><span style=\"font-size: 15px;\">\u3002<\/span><\/strong><span style=\"font-size: 15px;\">\u901a\u8fc7\u8fd9\u4e9b\u65b9\u6cd5\uff0c\u7528\u6237\u53ef\u4ee5\u9274\u5b9a\u51fa\u5728\u4e0d\u540c\u7ec6\u80de\u72b6\u6001\u6216\u5904\u7406\u6761\u4ef6\u4e0b\u8868\u8fbe\u663e\u8457\u53d8\u5316\u7684\u57fa\u56e0\uff0c\u8fd9\u4e9b\u57fa\u56e0\u5f80\u5f80\u4e0e\u7279\u5b9a\u7684\u751f\u7269\u5b66\u8fc7\u7a0b\u5bc6\u5207\u76f8\u5173\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u901a\u8fc7\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u627e\u5230\u4e00\u7cfb\u5217\u4e0e\u7ec6\u80de\u7c7b\u578b\u7279\u5f02\u6027\u6216\u73af\u5883\u54cd\u5e94\u76f8\u5173\u7684\u57fa\u56e0\u3002\u8fd9\u4e9b\u57fa\u56e0\u53ef\u80fd\u5728\u7279\u5b9a\u7684\u53d1\u80b2\u9636\u6bb5\u3001\u75be\u75c5\u72b6\u6001\u6216\u73af\u5883\u523a\u6fc0\u4e0b\u53d1\u6325\u7740\u5173\u952e\u4f5c\u7528\u3002\u901a\u8fc7\u6df1\u5165\u7814\u7a76\u8fd9\u4e9b\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\uff0c\u6211\u4eec\u53ef\u4ee5\u66f4\u597d\u5730\u7406\u89e3\u7ec6\u80de\u7684\u529f\u80fd\u548c\u8c03\u63a7\u673a\u5236\uff0c\u63ed\u793a\u5176\u5728\u4e0d\u540c\u751f\u7406\u548c\u75c5\u7406\u8fc7\u7a0b\u4e2d\u7684\u4f5c\u7528\u3002<\/span><\/p>\n<p><br mpa-from-tpl=\"t\" \/><\/p>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">\u6587\u7ae0\u5c0f\u7ed3<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"\">\n<p><span style=\"font-size: 15px;\">\u4ee5\u4e0a\u5c31\u662f\u5bf9R\u8bed\u8a00\u5305scater\u7684\u7b80\u5355\u4ecb\u7ecd\u5566\uff0cscater\u662f\u4e00\u4e2a\u5f3a\u5927\u7684R\u5305\uff0c\u4e3a\u751f\u7269\u5b66\u5bb6\u548c\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\u4eba\u5458\u5728\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u5206\u6790\u4e2d\u63d0\u4f9b\u4e86\u4e30\u5bcc\u7684\u529f\u80fd\u548c\u5de5\u5177\u3002\u5b83\u4e0d\u4ec5\u53ef\u4ee5\u5e2e\u52a9\u7528\u6237\u8fdb\u884c\u8d28\u91cf\u63a7\u5236\u3001\u5f52\u4e00\u5316\u548c\u53ef\u89c6\u5316\uff0c\u8fd8\u652f\u6301\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u4ece\u800c\u5728\u7814\u7a76\u7ec6\u80de\u591a\u6837\u6027\u3001\u529f\u80fd\u548c\u8c03\u63a7\u673a\u5236\u65b9\u9762\u63d0\u4f9b\u6709\u529b\u7684\u652f\u6301\u3002\u65e0\u8bba\u662f\u5728\u764c\u75c7\u7814\u7a76\u3001\u514d\u75ab\u5b66\u8fd8\u662f\u53d1\u80b2\u751f\u7269\u5b66\u9886\u57df\uff0cscater\u90fd\u5728\u5e2e\u52a9\u79d1\u7814\u4eba\u5458\u6df1\u5165\u6316\u6398\u5355\u7ec6\u80de\u6570\u636e\u80cc\u540e\u7684\u751f\u7269\u5b66\u4fe1\u606f\u3002<\/span><\/p>\n<p><strong><span style=\"font-size: 15px;\">\u5c0f\u4f19\u4f34\u4eec\uff0c\u4eca\u5929\u6709\u6ca1\u6709\u5b66\u5230\u65b0\u77e5\u8bc6\u5462\uff0c\u60f3\u8981\u7ee7\u7eed\u4e86\u89e3R\u8bed\u8a00\u5185\u5bb9\u53ef\u4ee5\u6301\u7eed\u5173\u6ce8\u5c0f\u679c\uff5e\u6216\u8005\u53ef\u4ee5\u626b\u63cf\u4e0b\u9762\u7684\u4e8c\u7ef4\u7801\u8fdb\u884c\u54a8\u8be2\u54e6~<\/span><\/strong><span style=\"font-size: 15px;\"><\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;text-align: left;\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"width: 520px;\">\n<section style=\"border-top: 1px solid rgb(0, 0, 0);width: 519.479px;float: left;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"margin-top: -3px;width: 6px;height: 6px;border-radius: 100%;float: right;background-color: rgb(46, 172, 109);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: -1em;padding: 3px 10px;display: inline-block;vertical-align: top;background-color: rgb(255, 255, 255);color: rgb(0, 154, 68);\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 15px;\" rel=\"noopener\"><span style=\"font-size: 15px;\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/span><\/a><br \/><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 15px;\" rel=\"noopener\"><span style=\"font-size: 15px;\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/span><\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491909&idx=1&sn=902e34bcf53e605fba2b457ec6a33e36&chksm=cf7f7180f808f8961542f458a49da14e85d8831449fb65fbf2623d55affc564686a5422f56fd&scene=21#wechat_redirect\" textvalue=\"3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 15px;\" rel=\"noopener\"><span style=\"font-size: 15px;\">3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8<\/span><\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491905&idx=1&sn=fb34ebd6282962c7803badaee7a39096&chksm=cf7f7184f808f8929608c0050344ad86193d0fc6455c1c18e16cc17bf4b37832b63d3b18284e&scene=21#wechat_redirect\" textvalue=\"4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 15px;\" rel=\"noopener\"><span style=\"font-size: 15px;\">4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b<\/span><\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493633&idx=1&sn=2263dfe7572c0bcf6816c2c1a37d79de&chksm=cf7f78c4f808f1d2209321d6ace39fde5fa60c0691b56f88eac21916f5a2872ce8ee345f1d5f&scene=21#wechat_redirect\" textvalue=\"\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\"><span style=\"font-size: 15px;\">5.\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01<\/span><\/a><br \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;text-align: left;\"><span style=\"font-size: 10.5pt;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493633&idx=1&sn=2263dfe7572c0bcf6816c2c1a37d79de&chksm=cf7f78c4f808f1d2209321d6ace39fde5fa60c0691b56f88eac21916f5a2872ce8ee345f1d5f&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" 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src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_IlmBAWe69EMUPMhNEgrv6NXX85lJxg-19.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwyrj7kbUR3ULG9ZUbLrLkgibzL8qUpQROfe8ebc3gNSnu1dIlmBAWe69EMUPMhNEgrv6NXX85lJxg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<\/section>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\"><strong>scRNA-seq\u6280\u672f<\/strong><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">scRNA-seq\u6280\u672f\u5df2\u7ecf\u6210\u4e3a\u4e86\u7814\u7a76\u5355\u4e2a\u7ec6\u80de\u57fa\u56e0\u8868\u8fbe\u7684\u4e3b\u8981\u624b\u6bb5\u3002\u7136\u800c\uff0c\u8fd9\u79cd\u9ad8\u7ef4\u5ea6\u7684\u6570\u636e\u9700\u8981\u590d\u6742\u7684\u5904\u7406\u548c\u5206\u6790\u65b9\u6cd5\u624d\u80fd\u4ece\u4e2d\u63d0\u53d6\u6709\u7528\u7684\u751f\u7269\u5b66\u4fe1\u606f\u3002\u5728\u9762\u5bf9\u5982\u6b64\u590d\u6742\u7684\u6311\u6218\u65f6\uff0cscater\u5305\u6210\u4e3a\u4e86\u7814\u7a76\u4eba\u5458\u4e0d\u53ef\u6216\u7f3a\u7684\u4f19\u4f34\u548c\u89e3\u51b3\u65b9\u6848\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\"><strong>scater<\/strong><\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">scater\u5305\u662f\u4e00\u4e2a\u5f3a\u5927\u7684\u5de5\u5177\u96c6\uff0c\u5b83\u4e3a\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u7684\u529f\u80fd\uff0c\u5e2e\u52a9\u7528\u6237\u4ece\u539f\u59cb\u6570\u636e\u4e2d\u63d0\u70bc\u51fa\u6709\u610f\u4e49\u7684\u7ed3\u679c\u3002\u5728\u8fd9\u4e2a\u5305\u7684\u5f15\u5bfc\u4e0b\uff0c\u7528\u6237\u80fd\u591f\u5728\u5206\u6790\u4e2d\u6267\u884c\u5173\u952e\u7684\u6b65\u9aa4\uff0c\u5982\u8d28\u91cf\u63a7\u5236\u3001\u5f52\u4e00\u5316\u3001\u53ef\u89c6\u5316\u548c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u4ece\u800c\u66f4\u597d\u5730\u7406\u89e3\u5355\u7ec6\u80de\u6570\u636e\u6240\u8574\u542b\u7684\u4fe1\u606f\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\"><strong>\u8d28\u91cf\u63a7\u5236<\/strong><\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">\u8d28\u91cf\u63a7\u5236\u662f\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u7684\u9996\u8981\u4efb\u52a1\u4e4b\u4e00\uff0c\u800cscater\u5305\u5728\u8fd9\u65b9\u9762\u53d1\u6325\u7740\u91cd\u8981\u4f5c\u7528\u3002\u901a\u8fc7\u8be5\u5305\uff0c\u7528\u6237\u53ef\u4ee5\u8bc6\u522b\u5e76\u79fb\u9664\u4f4e\u8d28\u91cf\u7ec6\u80de\uff0c\u4ece\u800c\u51cf\u5c11\u5206\u6790\u4e2d\u7684\u566a\u58f0\u548c\u8bef\u5dee\u3002\u8fd9\u4e00\u6b65\u7684\u5173\u952e\u5728\u4e8e\u4fdd\u969c\u540e\u7eed\u5206\u6790\u7684\u53ef\u9760\u6027\u548c\u51c6\u786e\u6027\uff0c\u4f7f\u5f97\u7528\u6237\u80fd\u591f\u66f4\u52a0\u81ea\u4fe1\u5730\u89e3\u91ca\u548c\u5e94\u7528\u5206\u6790\u7ed3\u679c\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">\u5f52\u4e00\u5316\u65b9\u6cd5<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">\u9664\u4e86\u8d28\u91cf\u63a7\u5236\uff0cscater\u5305\u8fd8\u63d0\u4f9b\u4e86\u591a\u79cd\u5f52\u4e00\u5316\u65b9\u6cd5\uff0c\u5141\u8bb8\u7528\u6237\u5728\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u8fdb\u884c\u6bd4\u8f83\u3002\u8fd9\u5728\u7814\u7a76\u4e2d\u5c24\u4e3a\u91cd\u8981\uff0c\u56e0\u4e3a\u4e0d\u540c\u5b9e\u9a8c\u6279\u6b21\u548c\u6280\u672f\u5dee\u5f02\u53ef\u80fd\u4f1a\u5f15\u5165\u504f\u5dee\u3002scater\u7684\u5f52\u4e00\u5316\u65b9\u6cd5\u53ef\u4ee5\u5e2e\u52a9\u7528\u6237\u6d88\u9664\u8fd9\u4e9b\u504f\u5dee\uff0c\u4f7f\u5f97\u6570\u636e\u66f4\u5177\u53ef\u6bd4\u6027\uff0c\u4ece\u800c\u66f4\u5bb9\u6613\u6355\u6349\u5230\u751f\u7269\u5b66\u4e0a\u771f\u5b9e\u7684\u5dee\u5f02\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u8981\u4f7f\u7528scater\u5305\uff0c\u53ef\u4ee5\u5728R\u4e2d\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\u8fdb\u884c\u5b89\u88c5\u548c\u52a0\u8f7d\uff1a<\/span><\/p>\n<p><span style=\"font-size: 15px;\"><em style=\"color: rgb(178, 178, 178);letter-spacing: 0.578px;white-space: normal;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 14px;letter-spacing: 0.476px;text-decoration: underline;\">\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c111\u201d\u9886\u53d6\u4ee3\u7801\uff0c\u4ee3\u7801\u7f16\u53f7\uff1a231008<\/span><\/span><\/em><\/span><\/p>\n<\/section>\n<section style=\"display: inline-block;width: 100%;vertical-align: top;overflow-x: auto;\" powered-by=\"xiumi.us\">\n<section style=\"overflow-y: hidden;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">> install.packages(<span class=\"code-snippet__string\">\"scater\"<\/span>) #\u5b89\u88c5scater\u8bed\u8a00\u5305<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> library(scater) #\u52a0\u8f7d\u8bed\u8a00\u5305<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">\u5728\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u5206\u6790\u4e2d\uff0c\u6570\u636e\u8d28\u91cf\u63a7\u5236\u548c\u9884\u5904\u7406\u662f\u786e\u4fdd\u51c6\u786e\u53ef\u9760\u5206\u6790\u7684\u57fa\u7840\u6b65\u9aa4\u3002<\/span><span style=\"font-size: 15px;color: rgb(255, 169, 0);\"><strong>scater\u4f5c\u4e3a\u4e00\u6b3e\u5f3a\u5927\u7684\u5206\u6790\u5de5\u5177\uff0c\u4e3a\u7528\u6237\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u9ad8\u6548\u7684\u529f\u80fd\u6765\u5904\u7406\u8fd9\u4e9b\u5173\u952e\u4efb\u52a1\u3002<\/strong><\/span><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">\u9996\u5148\uff0c\u6570\u636e\u8d28\u91cf\u63a7\u5236\u65b9\u9762\uff0cscater\u5141\u8bb8\u7528\u6237\u901a\u8fc7\u8ba1\u7b97\u591a\u79cd\u8d28\u91cf\u6307\u6807\uff0c\u5982\u7ec6\u80de\u7684\u6d4b\u5e8f\u6df1\u5ea6\u3001\u57fa\u56e0\u8868\u8fbe\u4e30\u5ea6\u548c\u7ec6\u80de\u7684dropout\u7387\uff0c\u6765\u8bc4\u4f30\u6570\u636e\u7684\u8d28\u91cf\u3002\u8fd9\u4e9b\u6307\u6807\u53ef\u4ee5\u5e2e\u52a9\u7528\u6237\u8bc6\u522b\u51fa\u4f4e\u8d28\u91cf\u7684\u7ec6\u80de\u548c\u57fa\u56e0\uff0c\u4ece\u800c\u4fdd\u7559\u9ad8\u8d28\u91cf\u7684\u6570\u636e\u7528\u4e8e\u540e\u7eed\u5206\u6790\u3002\u6b64\u5916\uff0cscater\u8fd8\u53ef\u4ee5\u751f\u6210\u8be6\u7ec6\u7684\u8d28\u91cf\u63a7\u5236\u62a5\u544a\uff0c\u5e2e\u52a9\u7528\u6237\u5168\u9762\u4e86\u89e3\u6570\u636e\u4e2d\u7684\u95ee\u9898\u548c\u8d8b\u52bf\u3002\u901a\u8fc7\u8bc6\u522b\u548c\u5254\u9664\u6280\u672f\u566a\u58f0\u548c\u6279\u6b21\u6548\u5e94\uff0cscater\u786e\u4fdd\u4e86\u540e\u7eed\u5206\u6790\u7684\u53ef\u9760\u6027\u548c\u51c6\u786e\u6027\u3002<\/span><\/p>\n<p><strong><span style=\"font-size: 15px;color: rgb(255, 169, 0);\">\u7ec6\u80de\u805a\u7c7b\u548c\u964d\u7ef4\u5206\u6790\u662f\u5355\u7ec6\u80de\u6570\u636e\u89e3\u6790\u7684\u5173\u952e\u6b65\u9aa4\uff0c\u6709\u52a9\u4e8e\u63ed\u793a\u6570\u636e\u4e2d\u7684\u7ec6\u80de\u7c7b\u578b\u548c\u7ed3\u6784\u3002<\/span><\/strong><span style=\"font-size: 15px;\">\u4e0e\u6b64\u7c7b\u4f3c\uff0cscater\u63d0\u4f9b\u4e86\u591a\u79cd\u805a\u7c7b\u7b97\u6cd5\u548c\u964d\u7ef4\u65b9\u6cd5\uff0c\u5982\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u3001t\u5206\u5e03\u968f\u673a\u90bb\u57df\u5d4c\u5165\uff08t-SNE\uff09\u548cUMAP\uff08Uniform Manifold Approximation and Projection\uff09\u3002\u8fd9\u4e9b\u65b9\u6cd5\u53ef\u4ee5\u5c06\u9ad8\u7ef4\u6570\u636e\u6620\u5c04\u5230\u66f4\u4f4e\u7ef4\u5ea6\u7684\u7a7a\u95f4\uff0c\u4f7f\u7528\u6237\u80fd\u591f\u5728\u4e8c\u7ef4\u6216\u4e09\u7ef4\u56fe\u4e2d\u89c2\u5bdf\u6570\u636e\u7684\u5206\u5e03\uff0c\u66f4\u597d\u5730\u7406\u89e3\u4e0d\u540c\u7ec6\u80de\u4e4b\u95f4\u7684\u76f8\u4f3c\u6027\u548c\u5dee\u5f02\u6027\u3002\u8fd9\u79cd\u7ec6\u80de\u805a\u7c7b\u548c\u964d\u7ef4\u5206\u6790\u4e3a\u6570\u636e\u7684\u53ef\u89c6\u5316\u63d0\u4f9b\u4e86\u6709\u529b\u652f\u6301\uff0c\u6709\u52a9\u4e8e\u7814\u7a76\u4eba\u5458\u66f4\u6df1\u5165\u5730\u63a2\u7d22\u7ec6\u80de\u7fa4\u7684\u7ed3\u6784\u548c\u7279\u5f81\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u662f\u4e86\u89e3\u4e0d\u540c\u6761\u4ef6\u4e0b\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u7684\u5173\u952e\u6b65\u9aa4\uff0c\u6709\u52a9\u4e8e\u8bc6\u522b\u5728\u7279\u5b9a\u751f\u7269\u5b66\u8fc7\u7a0b\u4e2d\u8d77\u5173\u952e\u4f5c\u7528\u7684\u57fa\u56e0\u3002<\/span><strong><span style=\"font-size: 15px;color: rgb(255, 169, 0);\">scater\u63d0\u4f9b\u4e86\u4e30\u5bcc\u7684\u7edf\u8ba1\u65b9\u6cd5\u548c\u53ef\u89c6\u5316\u5de5\u5177\uff0c\u4f7f\u7528\u6237\u80fd\u591f\u6bd4\u8f83\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u3001\u6761\u4ef6\u6216\u65f6\u95f4\u70b9\u4e4b\u95f4\u7684\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u5dee\u5f02<\/span><\/strong><span style=\"font-size: 15px;\">\u3002\u901a\u8fc7\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u6df1\u5165\u4e86\u89e3\u5728\u4e0d\u540c\u7ec6\u80de\u7fa4\u4e4b\u95f4\u7684\u751f\u7269\u5b66\u5dee\u5f02\uff0c\u4e3a\u8fdb\u4e00\u6b65\u7684\u673a\u5236\u89e3\u6790\u63d0\u4f9b\u7ebf\u7d22\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u5bf9\u4e8e\u7ec6\u80de\u53d1\u80b2\u8f68\u8ff9\u5206\u6790\uff0cscater\u662f\u4e00\u6b3e\u5f3a\u5927\u7684\u5de5\u5177\uff0c\u5b83\u80fd\u591f\u5e2e\u52a9\u7528\u6237\u63ed\u793a\u7ec6\u80de\u5206\u5316\u548c\u53d1\u80b2\u7684\u52a8\u6001\u53d8\u5316\u8fc7\u7a0b\u3002\u901a\u8fc7\u5efa\u7acb\u7ec6\u80de\u72b6\u6001\u7684\u53d1\u5c55\u8f68\u8ff9\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u66f4\u597d\u5730\u7406\u89e3\u7ec6\u80de\u72b6\u6001\u4e4b\u95f4\u7684\u5173\u7cfb\uff0c\u63a2\u7d22\u7ec6\u80de\u5206\u5316\u548c\u6f14\u5316\u7684\u8f68\u8ff9\uff0c\u4ece\u800c\u6df1\u5165\u4e86\u89e3\u53d1\u80b2\u8fc7\u7a0b\u4e2d\u7684\u91cd\u8981\u6027\u8d28\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">scater\u7684\u6570\u636e\u53ef\u89c6\u5316\u529f\u80fd\u4e3a\u7814\u7a76\u4eba\u5458\u5448\u73b0\u5206\u6790\u7ed3\u679c\u63d0\u4f9b\u4e86\u6709\u529b\u7684\u652f\u6301\u3002<span style=\"color: rgb(255, 169, 0);\"><strong>\u901a\u8fc7\u5185\u7f6e\u7684\u591a\u79cd\u6570\u636e\u53ef\u89c6\u5316\u5de5\u5177\uff0c\u5982\u6563\u70b9\u56fe\u3001\u70ed\u56fe\u548c\u8f68\u8ff9\u56fe\uff0c\u7528\u6237\u80fd\u591f\u4ee5\u76f4\u89c2\u7684\u65b9\u5f0f\u5c55\u793a\u4ed6\u4eec\u7684\u53d1\u73b0\u3002<\/strong><\/span>\u8fd9\u6837\u7684\u53ef\u89c6\u5316\u6709\u52a9\u4e8e\u66f4\u6e05\u6670\u5730\u4f20\u8fbe\u5206\u6790\u7ed3\u679c\uff0c\u5e76\u4e3a\u5176\u4ed6\u7814\u7a76\u4eba\u5458\u7406\u89e3\u5355\u7ec6\u80de\u6570\u636e\u7684\u6d1e\u5bdf\u63d0\u4f9b\u4e86\u65b9\u4fbf\u3002<\/span><\/p>\n<p><strong><span style=\"font-size: 15px;\">\u793a\u4f8b\uff1a<\/span><\/strong><span style=\"font-size: 15px;\"><\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u5f53\u4f7f\u7528scater\u5305\u65f6\uff0c\u901a\u5e38\u9700\u8981\u5bfc\u5165\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u96c6\uff0c\u5e76\u8fdb\u884c\u4e00\u7cfb\u5217\u7684\u9884\u5904\u7406\u3001\u53ef\u89c6\u5316\u548c\u5206\u6790\u6b65\u9aa4\u3002\u9996\u5148\uff0c\u5047\u8bbe\u4f60\u5df2\u7ecf\u5b89\u88c5\u4e86scater\u5305\u548c\u5176\u4ed6\u5fc5\u8981\u7684\u4f9d\u8d56\u9879\u3002\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u5c06\u52a0\u8f7d\u793a\u4f8b\u6570\u636e\u96c6\u5e76\u8fdb\u884c\u57fa\u672c\u7684\u5206\u6790\u548c\u53ef\u89c6\u5316\u3002<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"># \u52a0\u8f7d\u6240\u9700\u7684\u5e93<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> library(scater)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> library(SingleCellExperiment)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u4ecescater\u5305\u52a0\u8f7d\u793a\u4f8b\u6570\u636e\u96c6<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> data(\"sc_example_counts\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u5c06\u793a\u4f8b\u6570\u636e\u96c6\u8f6c\u6362\u4e3aSingleCellExperiment\u5bf9\u8c61<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- SingleCellExperiment(<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">+ assays = list(counts = as.matrix(sc_example_counts))<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">+ )<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u8fdb\u884c\u8d28\u91cf\u63a7\u5236<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- calculateQCMetrics(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u7ed8\u5236\u7ec6\u80de\u6570\u76ee\u548c\u57fa\u56e0\u6570\u76ee\u7684\u6563\u70b9\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> plotQC(sce, type = \"cell\", x = \"total_features_by_counts\", y = \"n_genes_by_counts\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u8fdb\u884c\u5f52\u4e00\u5316<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- normalize(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u4f7f\u7528PCA\u8fdb\u884c\u964d\u7ef4<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- runPCA(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u7ed8\u5236PCA\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> plotReducedDim(sce, dimred = \"PCA\")<\/span><\/code><\/pre>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7148014440433214\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_rKHqzp6wuMiamoFHSrVgl8N02O8Cpg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzhoNjktYmc4lN1vzqfM4E3Upw9xHdzWm9u4vmqBiaFBu2qg2rKHqzp6wuMiamoFHSrVgl8N02O8Cpg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"># \u8fdb\u884c\u805a\u7c7b<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- clusterCells(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u7ed8\u5236\u805a\u7c7b\u7ed3\u679c\u7684t-SNE\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- runTSNE(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> plotReducedDim(sce, dimred = \"TSNE\", label = \"clusters\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<section style=\"display: inline-block;width: 100%;vertical-align: top;overflow-x: auto;\" powered-by=\"xiumi.us\">\n<section style=\"overflow: hidden;width: 10%;max-width: 10% !important;\">\n<section style=\"display: inline-block;vertical-align: middle;width: 100%;\" powered-by=\"xiumi.us\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.49547920433996384\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_QQYvnB6VNO6xquMiahtFXszXrNBxUw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzhoNjktYmc4lN1vzqfM4E3BIzfe8iaOIbhBjNb3zw8wIgBKnQQYvnB6VNO6xquMiahtFXszXrNBxUw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"553\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"># \u8fdb\u884c\u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> sce <- DESeq(sce)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u7ed8\u5236\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684MA\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> plotMA(sce, contrast = \"condition_B_vs_A\")<\/span><\/code><\/pre>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6985294117647058\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_IcTTWBQkgzbA5cUDuqFicTEESeyjzQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzhoNjktYmc4lN1vzqfM4E3qZnIh7j4tP1PhdTEmEF1XMMNcIcTTWBQkgzbA5cUDuqFicTEESeyjzQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"272\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.44223826714801445\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_dVxLRWhIEHXBkzlbFxGGGURMWlHNng.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzhoNjktYmc4lN1vzqfM4E3R6svhtGJpCtDBaONWhVPM0AGdVxLRWhIEHXBkzlbFxGGGURMWlHNng\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"># \u7ed8\u5236\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u70ed\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">> plotHeatmap(sce, contrast = \"condition_B_vs_A\", cluster_rows = FALSE)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<section style=\"display: inline-block;width: 100%;vertical-align: top;overflow-x: auto;\" powered-by=\"xiumi.us\">\n<section style=\"overflow-y: hidden;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.3345388788426763\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_63bKxa8F5viaHqibEtsu3BQOhnGntQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzhoNjktYmc4lN1vzqfM4E3ywJEoPyVPdM2pw8sfjoMvUIGsf63bKxa8F5viaHqibEtsu3BQOhnGntQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"553\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">\u4e0a\u8ff0\u793a\u4f8b\u4ee3\u7801\u6f14\u793a\u4e86\u5982\u4f55\u4f7f\u7528scater\u5305\u52a0\u8f7d\u793a\u4f8b\u6570\u636e\u96c6\u3001\u8fdb\u884c\u8d28\u91cf\u63a7\u5236\u3001\u5f52\u4e00\u5316\u3001\u964d\u7ef4\u3001\u805a\u7c7b\u548c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u5e76\u751f\u6210\u7ec6\u80de\u6570\u76ee\u4e0e\u57fa\u56e0\u6570\u76ee\u7684\u6563\u70b9\u56fe\u3001PCA\u56fe\u3001t-SNE\u56fe\u3001MA\u56fe\u548c\u70ed\u56fe\u3002\u8fd9\u4e9b\u53ef\u89c6\u5316\u56fe\u8868\u6709\u52a9\u4e8e\u6211\u4eec\u76f4\u89c2\u5730\u7406\u89e3\u6570\u636e\u7684\u7279\u5f81\u548c\u6a21\u5f0f\uff0c\u8fdb\u800c\u4ece\u4e2d\u6316\u6398\u751f\u7269\u5b66\u76f8\u5173\u7684\u4fe1\u606f\u3002<\/span><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">\u7ed3\u8bba<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br mpa-from-tpl=\"t\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"font-size: 15px;\">\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u5728scRNA-seq\u6570\u636e\u5206\u6790\u4e2d\u626e\u6f14\u7740\u81f3\u5173\u91cd\u8981\u7684\u89d2\u8272\uff0c\u800cscater\u5305\u4e3a\u8fd9\u4e00\u73af\u8282\u63d0\u4f9b\u4e86\u5f3a\u5927\u7684\u652f\u6301\u3002<\/span><span style=\"color: rgb(255, 169, 0);\"><strong><span style=\"font-size: 15px;letter-spacing: 0.034em;\">\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u65e8\u5728\u8bc6\u522b\u5728\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u6216\u6761\u4ef6\u4e4b\u95f4\u8868\u8fbe\u6c34\u5e73\u663e\u8457\u53d8\u5316\u7684\u57fa\u56e0\uff0c\u8fd9\u6709\u52a9\u4e8e\u6211\u4eec\u66f4\u6df1\u5165\u5730\u7406\u89e3\u7ec6\u80de\u7684\u529f\u80fd\u548c\u8c03\u63a7\u673a\u5236<\/span><\/strong><\/span><strong><span style=\"font-size: 15px;\">\u3002<\/span><\/strong><span style=\"font-size: 15px;\">scater\u5305\u901a\u8fc7\u5b9e\u73b0\u591a\u79cd\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u65b9\u6cd5\uff0c\u5982edgeR\u548cDESeq2\uff0c\u4e3a\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4e86\u591a\u6837\u5316\u7684\u5de5\u5177\uff0c\u5e2e\u52a9\u4ed6\u4eec\u4ece\u6d77\u91cf\u7684\u6570\u636e\u4e2d\u6316\u6398\u51fa\u6709\u610f\u4e49\u7684\u751f\u7269\u5b66\u4fe1\u606f\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 15px;\">\u5728\u4f7f\u7528scater\u5305\u8fdb\u884c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u65f6\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u6839\u636e\u5b9e\u9645\u60c5\u51b5\u9009\u62e9\u9002\u5408\u7684\u65b9\u6cd5<\/span>\u3002<span style=\"color: rgb(255, 169, 0);\"><strong><span style=\"font-size: 15px;\">\u4f8b\u5982\uff0cedgeR\u548cDESeq2\u662f\u4e24\u79cd\u5e38\u7528\u7684\u7edf\u8ba1\u65b9\u6cd5\uff0c\u5b83\u4eec\u80fd\u591f\u8003\u8651\u5230\u6570\u636e\u7684\u79bb\u6563\u6027\u548c\u6279\u6b21\u6548\u5e94\uff0c\u4ece\u800c\u66f4\u51c6\u786e\u5730\u6355\u6349\u57fa\u56e0\u8868\u8fbe\u4e4b\u95f4\u7684\u5dee\u5f02<\/span><\/strong><\/span><strong><span style=\"font-size: 15px;\">\u3002<\/span><\/strong><span style=\"font-size: 15px;\">\u901a\u8fc7\u8fd9\u4e9b\u65b9\u6cd5\uff0c\u7528\u6237\u53ef\u4ee5\u9274\u5b9a\u51fa\u5728\u4e0d\u540c\u7ec6\u80de\u72b6\u6001\u6216\u5904\u7406\u6761\u4ef6\u4e0b\u8868\u8fbe\u663e\u8457\u53d8\u5316\u7684\u57fa\u56e0\uff0c\u8fd9\u4e9b\u57fa\u56e0\u5f80\u5f80\u4e0e\u7279\u5b9a\u7684\u751f\u7269\u5b66\u8fc7\u7a0b\u5bc6\u5207\u76f8\u5173\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u901a\u8fc7\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u627e\u5230\u4e00\u7cfb\u5217\u4e0e\u7ec6\u80de\u7c7b\u578b\u7279\u5f02\u6027\u6216\u73af\u5883\u54cd\u5e94\u76f8\u5173\u7684\u57fa\u56e0\u3002\u8fd9\u4e9b\u57fa\u56e0\u53ef\u80fd\u5728\u7279\u5b9a\u7684\u53d1\u80b2\u9636\u6bb5\u3001\u75be\u75c5\u72b6\u6001\u6216\u73af\u5883\u523a\u6fc0\u4e0b\u53d1\u6325\u7740\u5173\u952e\u4f5c\u7528\u3002\u901a\u8fc7\u6df1\u5165\u7814\u7a76\u8fd9\u4e9b\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\uff0c\u6211\u4eec\u53ef\u4ee5\u66f4\u597d\u5730\u7406\u89e3\u7ec6\u80de\u7684\u529f\u80fd\u548c\u8c03\u63a7\u673a\u5236\uff0c\u63ed\u793a\u5176\u5728\u4e0d\u540c\u751f\u7406\u548c\u75c5\u7406\u8fc7\u7a0b\u4e2d\u7684\u4f5c\u7528\u3002<\/span><\/p>\n<p><br mpa-from-tpl=\"t\" \/><\/p>\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section data-mpa-template=\"t\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 16px;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 0 0 auto;border-style: solid;border-width: 0px 0px 7px;border-bottom-color: rgb(175, 225, 255);min-width: 5%;height: auto;\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;font-size: 20px;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><strong mpa-from-tpl=\"t\" mpa-is-content=\"t\">\u6587\u7ae0\u5c0f\u7ed3<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: flex-end;\" mpa-from-tpl=\"t\">\n<section style=\"transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<section style=\"transform: translate3d(5px, 0px, 0px) rotateX(180deg);\" mpa-from-tpl=\"t\">\n<section style=\"display: inline-block;width: 6px;height: 6px;vertical-align: top;overflow: hidden;border-radius: 460px;background-color: rgb(244, 171, 189);\" mpa-from-tpl=\"t\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\" mpa-from-tpl=\"t\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"\">\n<p><span style=\"font-size: 15px;\">\u4ee5\u4e0a\u5c31\u662f\u5bf9R\u8bed\u8a00\u5305scater\u7684\u7b80\u5355\u4ecb\u7ecd\u5566\uff0cscater\u662f\u4e00\u4e2a\u5f3a\u5927\u7684R\u5305\uff0c\u4e3a\u751f\u7269\u5b66\u5bb6\u548c\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\u4eba\u5458\u5728\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u5206\u6790\u4e2d\u63d0\u4f9b\u4e86\u4e30\u5bcc\u7684\u529f\u80fd\u548c\u5de5\u5177\u3002\u5b83\u4e0d\u4ec5\u53ef\u4ee5\u5e2e\u52a9\u7528\u6237\u8fdb\u884c\u8d28\u91cf\u63a7\u5236\u3001\u5f52\u4e00\u5316\u548c\u53ef\u89c6\u5316\uff0c\u8fd8\u652f\u6301\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u4ece\u800c\u5728\u7814\u7a76\u7ec6\u80de\u591a\u6837\u6027\u3001\u529f\u80fd\u548c\u8c03\u63a7\u673a\u5236\u65b9\u9762\u63d0\u4f9b\u6709\u529b\u7684\u652f\u6301\u3002\u65e0\u8bba\u662f\u5728\u764c\u75c7\u7814\u7a76\u3001\u514d\u75ab\u5b66\u8fd8\u662f\u53d1\u80b2\u751f\u7269\u5b66\u9886\u57df\uff0cscater\u90fd\u5728\u5e2e\u52a9\u79d1\u7814\u4eba\u5458\u6df1\u5165\u6316\u6398\u5355\u7ec6\u80de\u6570\u636e\u80cc\u540e\u7684\u751f\u7269\u5b66\u4fe1\u606f\u3002<\/span><\/p>\n<p><strong><span style=\"font-size: 15px;\">\u5c0f\u4f19\u4f34\u4eec\uff0c\u4eca\u5929\u6709\u6ca1\u6709\u5b66\u5230\u65b0\u77e5\u8bc6\u5462\uff0c\u60f3\u8981\u7ee7\u7eed\u4e86\u89e3R\u8bed\u8a00\u5185\u5bb9\u53ef\u4ee5\u6301\u7eed\u5173\u6ce8\u5c0f\u679c\uff5e\u6216\u8005\u53ef\u4ee5\u626b\u63cf\u4e0b\u9762\u7684\u4e8c\u7ef4\u7801\u8fdb\u884c\u54a8\u8be2\u54e6~<\/span><\/strong><span style=\"font-size: 15px;\"><\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;text-align: left;\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"width: 520px;\">\n<section style=\"border-top: 1px solid rgb(0, 0, 0);width: 519.479px;float: left;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"margin-top: -3px;width: 6px;height: 6px;border-radius: 100%;float: right;background-color: rgb(46, 172, 109);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: -1em;padding: 3px 10px;display: inline-block;vertical-align: top;background-color: rgb(255, 255, 255);color: rgb(0, 154, 68);\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 15px;\" rel=\"noopener\"><span style=\"font-size: 15px;\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/span><\/a><br \/><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 15px;\" rel=\"noopener\"><span style=\"font-size: 15px;\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/span><\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a 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