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powered-by=\"xiumi.us\">\u4e34\u5e8a\u76f8\u5173\u5206\u671f\u662f\u6307\u5c06\u60a3\u8005\u7684\u75be\u75c5\u5206\u4e3a\u4e0d\u540c\u7684\u9636\u6bb5\u6216\u5206\u671f\uff0c\u4ee5\u4fbf\u8bc4\u4f30\u75be\u75c5\u7684\u4e25\u91cd\u7a0b\u5ea6\u3001\u9884\u540e\u4ee5\u53ca\u6307\u5bfc\u6cbb\u7597\u51b3\u7b56\u3002\u4e34\u5e8a\u76f8\u5173\u5206\u671f\u5728\u533b\u5b66\u9886\u57df\u4e2d\u5177\u6709\u91cd\u8981\u610f\u4e49\uff0c\u5177\u4f53\u7684\u5206\u6790\u65b9\u6cd5\u548c\u6b65\u9aa4\u53ef\u80fd\u4f1a\u56e0\u7814\u7a76\u76ee\u7684\u3001\u6570\u636e\u7c7b\u578b\u548c\u75be\u75c5\u7279\u70b9\u800c\u6709\u6240\u5dee\u5f02\u3002\u5728\u8fdb\u884c\u80bf\u7624\u4e34\u5e8a\u9636\u6bb5\u5206\u6790\u65f6\uff0c\u53ef\u4ee5\u6839\u636e\u5177\u4f53\u7684\u9700\u6c42\u9009\u62e9\u5408\u9002\u7684\u7edf\u8ba1\u65b9\u6cd5\u548cR\u5305\u6765\u8fdb\u884c\u5206\u6790\uff0c\u5e76\u7ed3\u5408\u4e34\u5e8a\u5b9e\u9645\u8fdb\u884c\u7ed3\u679c\u89e3\u91ca\u548c\u5e94\u7528\u3002\u6839\u636e\u5206\u6790\u7ed3\u679c\uff0c\u89e3\u91ca\u4e0d\u540c\u4e34\u5e8a\u5206\u671f\u7ec4\u522b\u4e4b\u95f4\u7684\u751f\u5b58\u5dee\u5f02\uff0c\u8bc4\u4f30\u4e34\u5e8a\u5206\u671f\u5728\u9884\u6d4b\u60a3\u8005\u751f\u5b58\u7387\u548c\u6cbb\u7597\u7b56\u7565\u5236\u5b9a\u4e2d\u7684\u610f\u4e49\u3002<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#if (!requireNamespace(\"BiocManager\", quietly = TRUE))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#if (!requireNamespace(\"BiocManager\", quietly = TRUE))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># install.packages(\"BiocManager\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#BiocManager::install(\"limma\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#install.packages(\"ggpubr\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">options(stringsAsFactors=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(limma)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">scoreFile=<span class=\"code-snippet__string\">\"scores.txt\"<\/span> <span class=\"code-snippet__comment\">#score\u6587\u4ef6\u6307\u5b9a\u5b58\u50a8\u5206\u6570\u6570\u636e\u7684\u6587\u4ef6\u540d\u3002<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.20176405733186328\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_QMaiaNtjLMWajtSRw3RRYjm7JeQFOw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUhDtnJb1oOOz3jWt3I7bLkjl6I9QQ8XwvbO8ziaZV4Kz9xKEQMaiaNtjLMWajtSRw3RRYjm7JeQFOw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"907\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">cliFile=<span class=\"code-snippet__string\">\"clinical.txt\"<\/span> <span class=\"code-snippet__comment\">#\u4e34\u5e8a\u6570\u636e\u6587\u4ef6\u6307\u5b9a\u5b58\u50a8\u4e34\u5e8a\u6570\u636e\u7684\u6587\u4ef6\u540d<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9061976549413735\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_j87aOQ6QnaoZibxS7aWdL6udysV0sg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUhDtnJb1oOOz3jWt3I7bLk1Qwl3ic1dtLJ8zA6Cibe4r1ceOZj87aOQ6QnaoZibxS7aWdL6udysV0sg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"597\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">\"C:\\Users\\lexb4\\Desktop\\TMEimmune\\10.cliCor\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fee\u6539\u5de5\u4f5c\u8bbe\u7f6e\u5de5\u4f5c\u76ee\u5f55\uff0c\u5373\u6570\u636e\u6587\u4ef6\u6240\u5728\u7684\u8def\u5f84\u3002\u76ee\u5f55<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6score\u6587\u4ef6\uff0c\u5e76\u5bf9\u8f93\u5165\u6587\u4ef6\u6574\u7406<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rt=read.table(scoreFile,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,header=<span class=\"code-snippet__built_in\">T<\/span>,check.names=<span class=\"code-snippet__built_in\">F<\/span>,row.names=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6score\u6587\u4ef6\u4e3a\u6570\u636e\u6846\uff0c\u5e76\u8bbe\u7f6e\u7b2c\u4e00\u5217\u4f5c\u4e3a\u884c\u540d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=as.matrix(rt)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06\u6570\u636e\u6846\u8f6c\u6362\u4e3a\u77e9\u9635\u683c\u5f0f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rownames(data)=gsub(<span class=\"code-snippet__string\">\"(.*?)\\-(.*?)\\-(.*?)\\-(.*?)\\-.*\"<\/span>,<span class=\"code-snippet__string\">\"\\1\\-\\2\\-\\3\"<\/span>,rownames(data))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9\u884c\u540d\u8fdb\u884c\u5904\u7406\uff0c\u63d0\u53d6\u6837\u672c\u540d\u79f0\u7684\u90e8\u5206\u4fe1\u606f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=avereps(data)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9\u91cd\u590d\u7684\u6837\u672c\u8fdb\u884c\u5e73\u5747\u5904\u7406\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6\u4e34\u5e8a\u6570\u636e\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cli=read.table(cliFile,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,header=<span class=\"code-snippet__built_in\">T<\/span>,check.names=<span class=\"code-snippet__built_in\">F<\/span>,row.names=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5408\u5e76\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">samSample=intersect(row.names(data),row.names(cli))<\/span><\/code><code><span class=\"code-snippet_outer\">data=data[samSample,]<\/span><\/code><code><span class=\"code-snippet_outer\">cli=cli[samSample,]<\/span><\/code><code><span class=\"code-snippet_outer\">rt=cbind(data,cli)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u627e\u5230\u6570\u636e\u548c\u4e34\u5e8a\u6570\u636e\u4e2d\u5171\u6709\u7684\u6837\u672c\u3002\u6839\u636e\u5171\u6709\u7684\u6837\u672c\u7b5b\u9009\u6570\u636e\u548c\u4e34\u5e8a\u6570\u636e\u3002#\u5c06\u6570\u636e\u548c\u4e34\u5e8a\u6570\u636e\u6309\u5217\u5408\u5e76\u4e3a\u4e00\u4e2a\u6570\u636e\u6846\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e34\u5e8a\u76f8\u5173\u6027\u5206\u6790\uff0c\u8f93\u51fa\u56fe\u5f62\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(clinical <span class=\"code-snippet__keyword\">in<\/span> colnames(rt[,<span class=\"code-snippet__number\">4<\/span>:ncol(rt)])){<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9\u4e34\u5e8a\u6570\u636e\u4e2d\u7684\u6bcf\u4e00\u5217\u8fdb\u884c\u5faa\u73af\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(score <span class=\"code-snippet__keyword\">in<\/span> colnames(rt[,<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">3<\/span>])){<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9\u5206\u6570\u6570\u636e\u4e2d\u7684\u6bcf\u4e00\u5217\u8fdb\u884c\u5faa\u73af\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=rt[<span class=\"code-snippet__built_in\">c<\/span>(score,clinical)]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4ece\u5408\u5e76\u7684\u6570\u636e\u6846\u4e2d\u9009\u62e9\u5206\u6570\u6570\u636e\u548c\u5f53\u524d\u4e34\u5e8a\u6570\u636e\u5217\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(data)=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"score\"<\/span>,<span class=\"code-snippet__string\">\"clinical\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u6570\u636e\u6846\u7684\u5217\u540d\u4e3a\"score\"\u548c\"clinical\"\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=data[(data[,<span class=\"code-snippet__string\">\"clinical\"<\/span>]!=<span class=\"code-snippet__string\">\"unknow\"<\/span>),]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5254\u9664\u4e34\u5e8a\u6570\u636e\u4e2d\u672a\u77e5\u7684\u6837\u672c\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u6bd4\u8f83\u7ec4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">group=levels(factor(data$clinical))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6\u4e34\u5e8a\u6570\u636e\u4e2d\u7684\u5206\u7ec4\u4fe1\u606f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data$clinical=factor(data$clinical, levels=group)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06\u4e34\u5e8a\u6570\u636e\u4e2d\u7684\u5206\u7ec4\u8f6c\u6362\u4e3a\u56e0\u5b50\u7c7b\u578b\uff0c\u5e76\u8bbe\u7f6e\u5206\u7ec4\u987a\u5e8f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">comp=combn(group,<span class=\"code-snippet__number\">2<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">my_comparisons=<span class=\"code-snippet__built_in\">list<\/span>()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> <span class=\"code-snippet__number\">1<\/span>:ncol(comp)){my_comparisons[[i]]<-comp[,i]}<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06\u6bd4\u8f83\u7ec4\u9010\u4e00\u6dfb\u52a0\u5230\u5217\u8868\u4e2d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236boxplot<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">boxplot=ggboxplot(data, x=<span class=\"code-snippet__string\">\"clinical\"<\/span>, y=<span class=\"code-snippet__string\">\"score\"<\/span>, color=<span class=\"code-snippet__string\">\"clinical\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">xlab=clinical,<\/span><\/code><code><span class=\"code-snippet_outer\">ylab=score,<\/span><\/code><code><span class=\"code-snippet_outer\">legend.title=clinical,<\/span><\/code><code><span class=\"code-snippet_outer\">add = <span class=\"code-snippet__string\">\"jitter\"<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">stat_compare_means(comparisons = my_comparisons)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u56fe\u7247<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pdf(file=paste0(score,<span class=\"code-snippet__string\">\".\"<\/span>,clinical,<span class=\"code-snippet__string\">\".pdf\"<\/span>),width=<span class=\"code-snippet__number\">5.5<\/span>,height=<span class=\"code-snippet__number\">5<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u7bb1\u7ebf\u56fe\u5e76\u8fdb\u884c\u7ec4\u95f4\u6bd4\u8f83\uff0c\u6700\u540e\u5c06\u7ed3\u679c\u4fdd\u5b58\u4e3aPDF\u6587\u4ef6\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">print(boxplot)<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\"><span style=\"text-indent: 0em;\">\u8fdb\u884c\u80bf\u7624\u4e34\u5e8a\u9636\u6bb5\u5206\u6790\u3002\u8bfb\u53d6\u4e86\u4e24\u4e2a\u6587\u4ef6\uff0c\u4e00\u4e2a\u662f\u5305\u542b\u80bf\u7624\u6837\u672c\u7684\u5206\u6570\u6570\u636e\u6587\u4ef6\uff0c\u53e6\u4e00\u4e2a\u662f\u5305\u542b\u4e0e\u8fd9\u4e9b\u6837\u672c\u76f8\u5173\u7684\u4e34\u5e8a\u6570\u636e\u6587\u4ef6\u3002\u9996\u5148\uff0c\u4ee3\u7801\u5bf9\u5206\u6570\u6570\u636e\u8fdb\u884c\u6574\u7406\u548c\u5904\u7406\uff0c\u63d0\u53d6\u6837\u672c\u540d\u79f0\u4fe1\u606f\u5e76\u53bb\u9664\u91cd\u590d\u7684\u6837\u672c\u3002\u7136\u540e\uff0c\u5c06\u5206\u6570\u6570\u636e\u548c\u4e34\u5e8a\u6570\u636e\u6309\u7167\u5171\u6709\u7684\u6837\u672c\u8fdb\u884c\u5408\u5e76\uff0c\u5f62\u6210\u4e00\u4e2a\u5305\u542b\u5206\u6570\u548c\u5bf9\u5e94\u4e34\u5e8a\u6570\u636e\u7684\u6570\u636e\u6846\u3002<\/span><br \/><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\"><span style=\"text-indent: 0em;\"><br \/><\/span><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u63a5\u4e0b\u6765\uff0c\u4ee3\u7801\u901a\u8fc7\u5faa\u73af\u904d\u5386\u6bcf\u4e2a\u4e34\u5e8a\u6570\u636e\u548c\u5206\u6570\u6570\u636e\u7684\u7ec4\u5408\uff0c\u8fdb\u884c\u4e34\u5e8a\u76f8\u5173\u6027\u5206\u6790\u548c\u56fe\u5f62\u7ed8\u5236\u3002\u5bf9\u4e8e\u6bcf\u4e2a\u7ec4\u5408\uff0c\u5b83\u9996\u5148\u7b5b\u9009\u51fa\u6709\u6548\u7684\u6570\u636e\uff0c\u5254\u9664\u4e34\u5e8a\u6570\u636e\u4e2d\u672a\u77e5\u7684\u6837\u672c\uff0c\u5e76\u63d0\u53d6\u5206\u7ec4\u4fe1\u606f\u3002\u7136\u540e\uff0c\u5b83\u6839\u636e\u5206\u7ec4\u4fe1\u606f\u8fdb\u884c\u7ec4\u95f4\u6bd4\u8f83\uff0c\u5e76\u7ed8\u5236\u7bb1\u7ebf\u56fe\u6765\u5c55\u793a\u5206\u6570\u6570\u636e\u5728\u4e0d\u540c\u4e34\u5e8a\u9636\u6bb5\u4e4b\u95f4\u7684\u5dee\u5f02\u3002\u7bb1\u7ebf\u56fe\u663e\u793a\u4e86\u5206\u7ec4\u4e4b\u95f4\u7684\u4e2d\u4f4d\u6570\u3001\u56db\u5206\u4f4d\u6570\u548c\u5f02\u5e38\u503c\u7684\u5206\u5e03\u60c5\u51b5\uff0c\u800c\u4e14\u901a\u8fc7\u6dfb\u52a0\u7edf\u8ba1\u6807\u8bb0\uff0c\u53ef\u4ee5\u53ef\u89c6\u5316\u5730\u663e\u793a\u51fa\u4e0d\u540c\u7ec4\u4e4b\u95f4\u7684\u663e\u8457\u6027\u5dee\u5f02\u3002<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\"><br \/><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u6700\u540e\uff0c\u4ee3\u7801\u5c06\u6bcf\u4e2a\u7ec4\u5408\u7684\u7bb1\u7ebf\u56fe\u4fdd\u5b58\u4e3aPDF\u6587\u4ef6\uff0c\u4ee5\u4fbf\u8fdb\u4e00\u6b65\u7684\u5206\u6790\u548c\u53ef\u89c6\u5316\u5c55\u793a\u3002<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u8fd9\u6bb5\u4ee3\u7801\u7684\u610f\u4e49\u5728\u4e8e\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u63a2\u7d22\u80bf\u7624\u4e34\u5e8a\u9636\u6bb5\u4e0e\u5206\u6570\u6570\u636e\u4e4b\u95f4\u7684\u5173\u7cfb\u3002\u901a\u8fc7\u4e34\u5e8a\u76f8\u5173\u6027\u5206\u6790\u548c\u56fe\u5f62\u5c55\u793a\uff0c\u53ef\u4ee5\u53d1\u73b0\u5206\u6570\u6570\u636e\u5728\u4e0d\u540c\u4e34\u5e8a\u9636\u6bb5\u4e4b\u95f4\u7684\u5dee\u5f02\uff0c\u8fdb\u800c\u5bf9\u80bf\u7624\u7684\u4e34\u5e8a\u7279\u5f81\u548c\u8fdb\u5c55\u72b6\u6001\u8fdb\u884c\u5206\u6790\u548c\u89e3\u91ca\u3002\u8fd9\u5bf9\u4e8e\u6df1\u5165\u7406\u89e3\u80bf\u7624\u53d1\u5c55\u8fc7\u7a0b\u4e2d\u7684\u751f\u7269\u5b66\u7279\u5f81\u548c\u9884\u540e\u60c5\u51b5\u5177\u6709\u91cd\u8981\u610f\u4e49\uff0c\u4e3a\u8fdb\u4e00\u6b65\u7684\u7814\u7a76\u548c\u6cbb\u7597\u51b3\u7b56\u63d0\u4f9b\u6709\u4ef7\u503c\u7684\u4fe1\u606f\u3002\u6709\u95ee\u9898\u53ef\u4ee5\u8054\u7cfb\u5c0f\u5e08\u59b9\u3002<\/p>\n<\/section>\n<p style=\"white-space: 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id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-08-27 19:00:38<\/em><\/div>\n<p><content><\/p>\n<div class=\"reprint\">\n<p>\u8f6c\u81ea\u516c\u4f17\u53f7\uff1aR\u8bed\u8a00\u5b66\u5f92<br \/><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg5MDk3Mzg4OA==&mid=2247485091&idx=1&sn=8d1ace8be9d283a4044e73578c3061ca\" target=\"_blank\" title=\"\u9605\u8bfb\u539f\u6587\" rel=\"noopener\">http:\/\/mp.weixin.qq.com\/s?__biz=Mzg5MDk3Mzg4OA==&mid=2247485091&idx=1&sn=8d1ace8be9d283a4044e73578c3061ca<\/a><\/p>\n<\/div>\n<section style=\"font-size: 16px;margin-bottom: 24px;\">\n<section style=\"text-align: center;justify-content: center;margin: 10px 0%;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" 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inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u84dd<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u5b57<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"color: rgb(255, 226, 93);font-size: 12px;\" powered-by=\"xiumi.us\">\n<p>\u2605<\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u5173<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u6ce8<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u6211<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"font-size: 19px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(249, 172, 172);background-color: rgb(249, 172, 172);width: 1.8em;height: 1.8em;line-height: 1.8em;border-radius: 100%;margin-left: auto;margin-right: auto;font-size: 17px;color: rgb(255, 255, 255);\">\n<p>\u4eec<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0em;white-space: normal;\" powered-by=\"xiumi.us\">\u4e34\u5e8a\u76f8\u5173\u5206\u671f\u662f\u6307\u5c06\u60a3\u8005\u7684\u75be\u75c5\u5206\u4e3a\u4e0d\u540c\u7684\u9636\u6bb5\u6216\u5206\u671f\uff0c\u4ee5\u4fbf\u8bc4\u4f30\u75be\u75c5\u7684\u4e25\u91cd\u7a0b\u5ea6\u3001\u9884\u540e\u4ee5\u53ca\u6307\u5bfc\u6cbb\u7597\u51b3\u7b56\u3002\u4e34\u5e8a\u76f8\u5173\u5206\u671f\u5728\u533b\u5b66\u9886\u57df\u4e2d\u5177\u6709\u91cd\u8981\u610f\u4e49\uff0c\u5177\u4f53\u7684\u5206\u6790\u65b9\u6cd5\u548c\u6b65\u9aa4\u53ef\u80fd\u4f1a\u56e0\u7814\u7a76\u76ee\u7684\u3001\u6570\u636e\u7c7b\u578b\u548c\u75be\u75c5\u7279\u70b9\u800c\u6709\u6240\u5dee\u5f02\u3002\u5728\u8fdb\u884c\u80bf\u7624\u4e34\u5e8a\u9636\u6bb5\u5206\u6790\u65f6\uff0c\u53ef\u4ee5\u6839\u636e\u5177\u4f53\u7684\u9700\u6c42\u9009\u62e9\u5408\u9002\u7684\u7edf\u8ba1\u65b9\u6cd5\u548cR\u5305\u6765\u8fdb\u884c\u5206\u6790\uff0c\u5e76\u7ed3\u5408\u4e34\u5e8a\u5b9e\u9645\u8fdb\u884c\u7ed3\u679c\u89e3\u91ca\u548c\u5e94\u7528\u3002\u6839\u636e\u5206\u6790\u7ed3\u679c\uff0c\u89e3\u91ca\u4e0d\u540c\u4e34\u5e8a\u5206\u671f\u7ec4\u522b\u4e4b\u95f4\u7684\u751f\u5b58\u5dee\u5f02\uff0c\u8bc4\u4f30\u4e34\u5e8a\u5206\u671f\u5728\u9884\u6d4b\u60a3\u8005\u751f\u5b58\u7387\u548c\u6cbb\u7597\u7b56\u7565\u5236\u5b9a\u4e2d\u7684\u610f\u4e49\u3002<\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#if (!requireNamespace(\"BiocManager\", quietly = TRUE))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#if (!requireNamespace(\"BiocManager\", quietly = TRUE))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># install.packages(\"BiocManager\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#BiocManager::install(\"limma\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#install.packages(\"ggpubr\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">options(stringsAsFactors=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(limma)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">scoreFile=<span class=\"code-snippet__string\">\"scores.txt\"<\/span> <span class=\"code-snippet__comment\">#score\u6587\u4ef6\u6307\u5b9a\u5b58\u50a8\u5206\u6570\u6570\u636e\u7684\u6587\u4ef6\u540d\u3002<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.20176405733186328\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_QMaiaNtjLMWajtSRw3RRYjm7JeQFOw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUhDtnJb1oOOz3jWt3I7bLkjl6I9QQ8XwvbO8ziaZV4Kz9xKEQMaiaNtjLMWajtSRw3RRYjm7JeQFOw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"907\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">cliFile=<span class=\"code-snippet__string\">\"clinical.txt\"<\/span> <span class=\"code-snippet__comment\">#\u4e34\u5e8a\u6570\u636e\u6587\u4ef6\u6307\u5b9a\u5b58\u50a8\u4e34\u5e8a\u6570\u636e\u7684\u6587\u4ef6\u540d<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9061976549413735\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_j87aOQ6QnaoZibxS7aWdL6udysV0sg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUhDtnJb1oOOz3jWt3I7bLk1Qwl3ic1dtLJ8zA6Cibe4r1ceOZj87aOQ6QnaoZibxS7aWdL6udysV0sg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"597\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">\"C:\\Users\\lexb4\\Desktop\\TMEimmune\\10.cliCor\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fee\u6539\u5de5\u4f5c\u8bbe\u7f6e\u5de5\u4f5c\u76ee\u5f55\uff0c\u5373\u6570\u636e\u6587\u4ef6\u6240\u5728\u7684\u8def\u5f84\u3002\u76ee\u5f55<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6score\u6587\u4ef6\uff0c\u5e76\u5bf9\u8f93\u5165\u6587\u4ef6\u6574\u7406<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rt=read.table(scoreFile,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,header=<span class=\"code-snippet__built_in\">T<\/span>,check.names=<span class=\"code-snippet__built_in\">F<\/span>,row.names=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6score\u6587\u4ef6\u4e3a\u6570\u636e\u6846\uff0c\u5e76\u8bbe\u7f6e\u7b2c\u4e00\u5217\u4f5c\u4e3a\u884c\u540d\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=as.matrix(rt)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06\u6570\u636e\u6846\u8f6c\u6362\u4e3a\u77e9\u9635\u683c\u5f0f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">rownames(data)=gsub(<span class=\"code-snippet__string\">\"(.*?)\\-(.*?)\\-(.*?)\\-(.*?)\\-.*\"<\/span>,<span class=\"code-snippet__string\">\"\\1\\-\\2\\-\\3\"<\/span>,rownames(data))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9\u884c\u540d\u8fdb\u884c\u5904\u7406\uff0c\u63d0\u53d6\u6837\u672c\u540d\u79f0\u7684\u90e8\u5206\u4fe1\u606f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=avereps(data)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9\u91cd\u590d\u7684\u6837\u672c\u8fdb\u884c\u5e73\u5747\u5904\u7406\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6\u4e34\u5e8a\u6570\u636e\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cli=read.table(cliFile,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,header=<span class=\"code-snippet__built_in\">T<\/span>,check.names=<span class=\"code-snippet__built_in\">F<\/span>,row.names=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5408\u5e76\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">samSample=intersect(row.names(data),row.names(cli))<\/span><\/code><code><span class=\"code-snippet_outer\">data=data[samSample,]<\/span><\/code><code><span class=\"code-snippet_outer\">cli=cli[samSample,]<\/span><\/code><code><span class=\"code-snippet_outer\">rt=cbind(data,cli)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u627e\u5230\u6570\u636e\u548c\u4e34\u5e8a\u6570\u636e\u4e2d\u5171\u6709\u7684\u6837\u672c\u3002\u6839\u636e\u5171\u6709\u7684\u6837\u672c\u7b5b\u9009\u6570\u636e\u548c\u4e34\u5e8a\u6570\u636e\u3002#\u5c06\u6570\u636e\u548c\u4e34\u5e8a\u6570\u636e\u6309\u5217\u5408\u5e76\u4e3a\u4e00\u4e2a\u6570\u636e\u6846\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e34\u5e8a\u76f8\u5173\u6027\u5206\u6790\uff0c\u8f93\u51fa\u56fe\u5f62\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(clinical <span class=\"code-snippet__keyword\">in<\/span> colnames(rt[,<span class=\"code-snippet__number\">4<\/span>:ncol(rt)])){<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9\u4e34\u5e8a\u6570\u636e\u4e2d\u7684\u6bcf\u4e00\u5217\u8fdb\u884c\u5faa\u73af\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(score <span class=\"code-snippet__keyword\">in<\/span> colnames(rt[,<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">3<\/span>])){<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5bf9\u5206\u6570\u6570\u636e\u4e2d\u7684\u6bcf\u4e00\u5217\u8fdb\u884c\u5faa\u73af\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=rt[<span class=\"code-snippet__built_in\">c<\/span>(score,clinical)]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4ece\u5408\u5e76\u7684\u6570\u636e\u6846\u4e2d\u9009\u62e9\u5206\u6570\u6570\u636e\u548c\u5f53\u524d\u4e34\u5e8a\u6570\u636e\u5217\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(data)=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"score\"<\/span>,<span class=\"code-snippet__string\">\"clinical\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u6570\u636e\u6846\u7684\u5217\u540d\u4e3a\"score\"\u548c\"clinical\"\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=data[(data[,<span class=\"code-snippet__string\">\"clinical\"<\/span>]!=<span class=\"code-snippet__string\">\"unknow\"<\/span>),]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5254\u9664\u4e34\u5e8a\u6570\u636e\u4e2d\u672a\u77e5\u7684\u6837\u672c\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u6bd4\u8f83\u7ec4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">group=levels(factor(data$clinical))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6\u4e34\u5e8a\u6570\u636e\u4e2d\u7684\u5206\u7ec4\u4fe1\u606f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data$clinical=factor(data$clinical, levels=group)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06\u4e34\u5e8a\u6570\u636e\u4e2d\u7684\u5206\u7ec4\u8f6c\u6362\u4e3a\u56e0\u5b50\u7c7b\u578b\uff0c\u5e76\u8bbe\u7f6e\u5206\u7ec4\u987a\u5e8f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">comp=combn(group,<span class=\"code-snippet__number\">2<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">my_comparisons=<span class=\"code-snippet__built_in\">list<\/span>()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> <span class=\"code-snippet__number\">1<\/span>:ncol(comp)){my_comparisons[[i]]<-comp[,i]}<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06\u6bd4\u8f83\u7ec4\u9010\u4e00\u6dfb\u52a0\u5230\u5217\u8868\u4e2d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236boxplot<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">boxplot=ggboxplot(data, x=<span class=\"code-snippet__string\">\"clinical\"<\/span>, y=<span class=\"code-snippet__string\">\"score\"<\/span>, color=<span class=\"code-snippet__string\">\"clinical\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">xlab=clinical,<\/span><\/code><code><span class=\"code-snippet_outer\">ylab=score,<\/span><\/code><code><span class=\"code-snippet_outer\">legend.title=clinical,<\/span><\/code><code><span class=\"code-snippet_outer\">add = <span class=\"code-snippet__string\">\"jitter\"<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">stat_compare_means(comparisons = my_comparisons)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u56fe\u7247<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pdf(file=paste0(score,<span class=\"code-snippet__string\">\".\"<\/span>,clinical,<span class=\"code-snippet__string\">\".pdf\"<\/span>),width=<span class=\"code-snippet__number\">5.5<\/span>,height=<span class=\"code-snippet__number\">5<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u7bb1\u7ebf\u56fe\u5e76\u8fdb\u884c\u7ec4\u95f4\u6bd4\u8f83\uff0c\u6700\u540e\u5c06\u7ed3\u679c\u4fdd\u5b58\u4e3aPDF\u6587\u4ef6\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">print(boxplot)<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\"><span style=\"text-indent: 0em;\">\u8fdb\u884c\u80bf\u7624\u4e34\u5e8a\u9636\u6bb5\u5206\u6790\u3002\u8bfb\u53d6\u4e86\u4e24\u4e2a\u6587\u4ef6\uff0c\u4e00\u4e2a\u662f\u5305\u542b\u80bf\u7624\u6837\u672c\u7684\u5206\u6570\u6570\u636e\u6587\u4ef6\uff0c\u53e6\u4e00\u4e2a\u662f\u5305\u542b\u4e0e\u8fd9\u4e9b\u6837\u672c\u76f8\u5173\u7684\u4e34\u5e8a\u6570\u636e\u6587\u4ef6\u3002\u9996\u5148\uff0c\u4ee3\u7801\u5bf9\u5206\u6570\u6570\u636e\u8fdb\u884c\u6574\u7406\u548c\u5904\u7406\uff0c\u63d0\u53d6\u6837\u672c\u540d\u79f0\u4fe1\u606f\u5e76\u53bb\u9664\u91cd\u590d\u7684\u6837\u672c\u3002\u7136\u540e\uff0c\u5c06\u5206\u6570\u6570\u636e\u548c\u4e34\u5e8a\u6570\u636e\u6309\u7167\u5171\u6709\u7684\u6837\u672c\u8fdb\u884c\u5408\u5e76\uff0c\u5f62\u6210\u4e00\u4e2a\u5305\u542b\u5206\u6570\u548c\u5bf9\u5e94\u4e34\u5e8a\u6570\u636e\u7684\u6570\u636e\u6846\u3002<\/span><br \/><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\"><span style=\"text-indent: 0em;\"><br \/><\/span><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u63a5\u4e0b\u6765\uff0c\u4ee3\u7801\u901a\u8fc7\u5faa\u73af\u904d\u5386\u6bcf\u4e2a\u4e34\u5e8a\u6570\u636e\u548c\u5206\u6570\u6570\u636e\u7684\u7ec4\u5408\uff0c\u8fdb\u884c\u4e34\u5e8a\u76f8\u5173\u6027\u5206\u6790\u548c\u56fe\u5f62\u7ed8\u5236\u3002\u5bf9\u4e8e\u6bcf\u4e2a\u7ec4\u5408\uff0c\u5b83\u9996\u5148\u7b5b\u9009\u51fa\u6709\u6548\u7684\u6570\u636e\uff0c\u5254\u9664\u4e34\u5e8a\u6570\u636e\u4e2d\u672a\u77e5\u7684\u6837\u672c\uff0c\u5e76\u63d0\u53d6\u5206\u7ec4\u4fe1\u606f\u3002\u7136\u540e\uff0c\u5b83\u6839\u636e\u5206\u7ec4\u4fe1\u606f\u8fdb\u884c\u7ec4\u95f4\u6bd4\u8f83\uff0c\u5e76\u7ed8\u5236\u7bb1\u7ebf\u56fe\u6765\u5c55\u793a\u5206\u6570\u6570\u636e\u5728\u4e0d\u540c\u4e34\u5e8a\u9636\u6bb5\u4e4b\u95f4\u7684\u5dee\u5f02\u3002\u7bb1\u7ebf\u56fe\u663e\u793a\u4e86\u5206\u7ec4\u4e4b\u95f4\u7684\u4e2d\u4f4d\u6570\u3001\u56db\u5206\u4f4d\u6570\u548c\u5f02\u5e38\u503c\u7684\u5206\u5e03\u60c5\u51b5\uff0c\u800c\u4e14\u901a\u8fc7\u6dfb\u52a0\u7edf\u8ba1\u6807\u8bb0\uff0c\u53ef\u4ee5\u53ef\u89c6\u5316\u5730\u663e\u793a\u51fa\u4e0d\u540c\u7ec4\u4e4b\u95f4\u7684\u663e\u8457\u6027\u5dee\u5f02\u3002<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\"><br \/><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u6700\u540e\uff0c\u4ee3\u7801\u5c06\u6bcf\u4e2a\u7ec4\u5408\u7684\u7bb1\u7ebf\u56fe\u4fdd\u5b58\u4e3aPDF\u6587\u4ef6\uff0c\u4ee5\u4fbf\u8fdb\u4e00\u6b65\u7684\u5206\u6790\u548c\u53ef\u89c6\u5316\u5c55\u793a\u3002<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u8fd9\u6bb5\u4ee3\u7801\u7684\u610f\u4e49\u5728\u4e8e\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u63a2\u7d22\u80bf\u7624\u4e34\u5e8a\u9636\u6bb5\u4e0e\u5206\u6570\u6570\u636e\u4e4b\u95f4\u7684\u5173\u7cfb\u3002\u901a\u8fc7\u4e34\u5e8a\u76f8\u5173\u6027\u5206\u6790\u548c\u56fe\u5f62\u5c55\u793a\uff0c\u53ef\u4ee5\u53d1\u73b0\u5206\u6570\u6570\u636e\u5728\u4e0d\u540c\u4e34\u5e8a\u9636\u6bb5\u4e4b\u95f4\u7684\u5dee\u5f02\uff0c\u8fdb\u800c\u5bf9\u80bf\u7624\u7684\u4e34\u5e8a\u7279\u5f81\u548c\u8fdb\u5c55\u72b6\u6001\u8fdb\u884c\u5206\u6790\u548c\u89e3\u91ca\u3002\u8fd9\u5bf9\u4e8e\u6df1\u5165\u7406\u89e3\u80bf\u7624\u53d1\u5c55\u8fc7\u7a0b\u4e2d\u7684\u751f\u7269\u5b66\u7279\u5f81\u548c\u9884\u540e\u60c5\u51b5\u5177\u6709\u91cd\u8981\u610f\u4e49\uff0c\u4e3a\u8fdb\u4e00\u6b65\u7684\u7814\u7a76\u548c\u6cbb\u7597\u51b3\u7b56\u63d0\u4f9b\u6709\u4ef7\u503c\u7684\u4fe1\u606f\u3002\u6709\u95ee\u9898\u53ef\u4ee5\u8054\u7cfb\u5c0f\u5e08\u59b9\u3002<\/p>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><strong>\u6765\u7ed9\u5927\u5bb6\u5c55\u793a\u5c55\u793a\u7ed3\u679c\u56fe\u5427\u3002<\/strong><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.097165991902834\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_r322eFtG54OiaRbjSaQdfzykhHicew.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUhDtnJb1oOOz3jWt3I7bLkIpmO93xDHONBMJaofIX59zmqonr322eFtG54OiaRbjSaQdfzykhHicew\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"247\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9218500797448166\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_mAr8aLzuegPa8rVfuyQFnbvsm57AfA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUhDtnJb1oOOz3jWt3I7bLkhISxU8RpJtAZ6ZxnQjShTueYmAr8aLzuegPa8rVfuyQFnbvsm57AfA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"627\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8726993865030674\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_HOIcKibo836GyZ9WxFugo0zkTGQJZA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEUhDtnJb1oOOz3jWt3I7bLkwrnhCSjV52BY1oI18Hw6rdZ6GHOIcKibo836GyZ9WxFugo0zkTGQJZA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"652\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: 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