<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner,body{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media{padding:20px;overflow:hidden;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}@media screen and (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media_content{overflow:hidden;color:#333;font-size:17px;word-wrap:break-word;-webkit-hyphens:auto;-ms-hyphens:auto;hyphens:auto;text-align:justify;position:relative;z-index:0}.rich_media_content *{max-width:100%!important;box-sizing:border-box!important;-webkit-box-sizing:border-box!important;word-wrap:break-word!important}.rich_media_content p{clear:both;min-height:1em}.rich_media_content em{font-style:italic}.rich_media_content fieldset{min-width:0}.rich_media_content .list-paddingleft-1,.rich_media_content .list-paddingleft-2,.rich_media_content .list-paddingleft-3{padding-left:2.2em}.rich_media_content .list-paddingleft-1 .list-paddingleft-2,.rich_media_content .list-paddingleft-2 .list-paddingleft-2,.rich_media_content .list-paddingleft-3 .list-paddingleft-2{padding-left:30px}.rich_media_content .list-paddingleft-1{padding-left:1.2em}.rich_media_content .list-paddingleft-3{padding-left:3.2em}.rich_media_content .code-snippet,.rich_media_content .code-snippet__fix{max-width:1000%!important}.rich_media_content .code-snippet *,.rich_media_content .code-snippet__fix *{max-width:1000%!important}.rich_media_title{font-size:22px;line-height:1.4;margin-bottom:13px;padding-bottom:13px;border-bottom:1px solid #e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag .code-snippet__attr{color:#0e9ce5}.code-snippet__string,.code-snippet__doctag{color:#d14}.code-snippet__title,.code-snippet__section,.code-snippet__selector-id{color:#d14}.code-snippet__subst{font-weight:normal}.code-snippet__type,.code-snippet__class .code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u751f\u4fe1\u6587\u7ae0\u5fc5\u5907\u5206\u6790\u5185\u5bb9\uff0c\u5bf9\u4e9a\u578b\u5229\u7528MCPcounter\u548cCIBERSORT\u4e24\u79cd\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790\",\"time\":\"2023-08-21 19:02:53\",\"timeStamp\":\"1692615773\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491566&idx=5&sn=852ad8c59fcfe0b38977532fd727c462&chksm=cf7c8f2bf80b063df6dbe4849a99497b8edf7232f7f1508e0a110a922b60d7f8cb76ba3adc6e#rd\" target=\"_blank\" rel=\"noopener\">\u751f\u4fe1\u6587\u7ae0\u5fc5\u5907\u5206\u6790\u5185\u5bb9\uff0c\u5bf9\u4e9a\u578b\u5229\u7528MCPcounter\u548cCIBERSORT\u4e24\u79cd\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u82b1 <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-08-21 19:02:53<\/em><\/div>\n<p><content><\/p>\n<div class=\"reprint\">\n<p>\u8f6c\u81ea\u516c\u4f17\u53f7\uff1a\u751f\u4fe1\u82b1<br \/><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484214&idx=1&sn=9a5856b790d928fbaa6657c4c6450175\" target=\"_blank\" title=\"\u9605\u8bfb\u539f\u6587\" rel=\"noopener\">http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484214&idx=1&sn=9a5856b790d928fbaa6657c4c6450175<\/a><\/p>\n<\/div>\n<section style=\"font-size: 14px;margin-bottom: 24px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(65, 128, 216);\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;padding-left: 12px;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);\" powered-by=\"xiumi.us\">\n<p><strong>\u70b9\u51fb\u84dd\u5b57 \u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(201, 229, 70);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u4eca\u5929\u5c0f\u82b1\u5e26\u6765\u7684\u5206\u4eab\u4e3a\u9996\u5148\u5229\u7528ConsensusCluster\u5bf9\u80bf\u7624\u6837\u672c\u8fdb\u884c\u5206\u578b\uff0c\u7136\u540e\u5bf9\u4e0d\u540c\u4e9a\u578b\u5229\u7528MCPcounter\u548cCIBERSORT\u4e24\u79cd\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\u6700\u540e\u5bf9\u4e0d\u540c\u4e9a\u578b\u4e4b\u95f4\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u8fdb\u884c\u663e\u8457\u5dee\u5f02\u5206\u6790\uff0c\u8be5\u5206\u6790\u5728\u751f\u4fe1\u6587\u7ae0\u4e2d\u51fa\u73b0\u7684\u9891\u7387\u975e\u5e38\u9ad8\uff0c\u503c\u5f97\u5c0f\u4f19\u4f34\u4eec\u5b66\u4e60\u55f7\uff0c\u63a5\u4e0b\u6765\u8ddf\u7740\u5c0f\u82b1\u5f00\u542f\u4eca\u5929\u7684\u5b66\u4e60\u4e4b\u65c5\u5427\uff01<br \/><\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;\" powered-by=\"xiumi.us\">\n<p><strong>\u51c6\u5907\u9700\u8981\u7684R\u5305<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5b89\u88c5\u9700\u8981\u7684R\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"tidyverse\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"devtools\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(devtools)<\/span><\/code><code><span class=\"code-snippet_outer\">install_github(<span class=\"code-snippet__string\">\"ebecht\/MCPcounter\"<\/span>,ref=<span class=\"code-snippet__string\">\"master\"<\/span>, subdir=<span class=\"code-snippet__string\">\"Source\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggplot2\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggsci\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">BiocManager::install(<span class=\"code-snippet__string\">\"ConsensusClusterPlus\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u52a0\u8f7d\u9700\u8981\u7684R\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(MCPcounter)<\/span><\/code><code><span class=\"code-snippet_outer\">library(reshape2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(tidyverse)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggsci)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ConsensusClusterPlus)<\/span><\/code><code><span class=\"code-snippet_outer\">source(<span class=\"code-snippet__string\">'Cibersort.R'<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;\" powered-by=\"xiumi.us\">\n<p><strong>ConsensusCluster\u4e00\u81f4\u6027\u805a\u7c7b\u5206\u6790<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7b5b\u9009\u51fa\u7684\u6709\u9884\u540e\u7684TEX\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6587\u4ef6\uff0c\u884c\u540d\u4e3a\u57fa\u56e0\u540d\uff0c\u5217\u4e3a\u6837\u672c\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">expr1<- read.table(file = <span class=\"code-snippet__string\">\"expr_cluster.txt\"<\/span>,<span class=\"code-snippet__comment\">#\u5de5\u4f5c\u76ee\u5f55\u4e0b\u7684\u6587\u4ef6\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">header = <span class=\"code-snippet__built_in\">T<\/span>,<span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u6709\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sep = <span class=\"code-snippet__string\">\"t\"<\/span>,<span class=\"code-snippet__comment\">#\u5206\u9694\u7b26<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">row.names = <span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u884c\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">check.names=<span class=\"code-snippet__built_in\">F<\/span>)<span class=\"code-snippet__comment\">#\u4e0d\u68c0\u67e5\u884c\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dir.create(<span class=\"code-snippet__string\">'ConsensusCluster\/'<\/span>)<span class=\"code-snippet__comment\">#\u6784\u5efa\u6587\u4ef6\u5939<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#ConsensusCluster\u4e00\u81f4\u6027\u805a\u7c7b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">results = ConsensusClusterPlus(as.matrix(expr1),<\/span><\/code><code><span class=\"code-snippet_outer\">maxK=<span class=\"code-snippet__number\">6<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">reps=<span class=\"code-snippet__number\">100<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">pItem=<span class=\"code-snippet__number\">0.8<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">pFeature=<span class=\"code-snippet__number\">1<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">title=<span class=\"code-snippet__string\">'ConsensusCluster\/'<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">clusterAlg=<span class=\"code-snippet__string\">\"km\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">distance=<span class=\"code-snippet__string\">\"euclidean\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">seed=<span class=\"code-snippet__number\">123456<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">plot=<span class=\"code-snippet__string\">\"pdf\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">icl <- calcICL(results,title = <span class=\"code-snippet__string\">'ConsensusCluster\/'<\/span>,plot = <span class=\"code-snippet__string\">'pdf'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">Kvec = <span class=\"code-snippet__number\">2<\/span>:<span class=\"code-snippet__number\">6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">x1 = <span class=\"code-snippet__number\">0.1<\/span>; x2 = <span class=\"code-snippet__number\">0.6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">PAC = <span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__literal\">NA<\/span>,<span class=\"code-snippet__built_in\">length<\/span>(Kvec))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">names<\/span>(PAC) = paste(<span class=\"code-snippet__string\">\"K=\"<\/span>,Kvec,sep=<span class=\"code-snippet__string\">\"\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> Kvec){<\/span><\/code><code><span class=\"code-snippet_outer\">M = results[[i]]$consensusMatrix<\/span><\/code><code><span class=\"code-snippet_outer\">Fn = ecdf(M[lower.tri(M)])<\/span><\/code><code><span class=\"code-snippet_outer\">PAC[i-<span class=\"code-snippet__number\">1<\/span>] = Fn(x2) - Fn(x1)<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">optK = Kvec[which.min(PAC)]<\/span><\/code><code><span class=\"code-snippet_outer\">optK<\/span><\/code><code><span class=\"code-snippet_outer\">PAC <- as.data.frame(PAC)<\/span><\/code><code><span class=\"code-snippet_outer\">PAC$K <- 2:<span class=\"code-snippet__number\">6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236PAC\u9a8c\u8bc1\u66f2\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggplot(PAC,aes(factor(K),PAC,group=<span class=\"code-snippet__number\">1<\/span>))+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_line()+<\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw()+theme(panel.grid = element_blank())+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_point(size=<span class=\"code-snippet__number\">4<\/span>,shape=<span class=\"code-snippet__number\">21<\/span>,color=<span class=\"code-snippet__string\">'darkred'<\/span>,fill=<span class=\"code-snippet__string\">'skyblue'<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">ylab(<span class=\"code-snippet__string\">'Proportion of ambiguous clustering'<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">xlab(<span class=\"code-snippet__string\">'Cluster number K'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(<span class=\"code-snippet__string\">\"PAC.pdf\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6700\u5c0f\u503c\u4e5f\u5c31\u662f\u6700\u4f73K\uff0c\u4ecd\u7136\u4e3a3.<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6\u5206\u578b\u4fe1\u606f\uff0ck=3<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">clusterNum=<span class=\"code-snippet__number\">3<\/span> <span class=\"code-snippet__comment\">#k\u503c\u53d8\u5316\u8c03\u6574<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cluster=results[[clusterNum]][[<span class=\"code-snippet__string\">\"consensusClass\"<\/span>]]<\/span><\/code><code><span class=\"code-snippet_outer\">sub <- data.frame(Sample=<span class=\"code-snippet__built_in\">names<\/span>(cluster),Cluster=cluster)<\/span><\/code><code><span class=\"code-snippet_outer\">sub$Cluster <- paste0(<span class=\"code-snippet__string\">'C'<\/span>,sub$Cluster)<\/span><\/code><code><span class=\"code-snippet_outer\">table(sub$Cluster)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(sub,file = <span class=\"code-snippet__string\">\"K3.txt\"<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,<span class=\"code-snippet__built_in\">quote<\/span>=<span class=\"code-snippet__built_in\">F<\/span>)<span class=\"code-snippet__comment\">#\u8f93\u51fa\u7ed3\u679c<\/span><\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 11px;\"><strong>\u4e9a\u578b\u95f4\u5229\u7528MCPCounter\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u663e\u8457\u5dee\u5f02\u5206\u6790<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#EXP.txt\uff0c\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6587\u4ef6\uff0c\u884c\u540d\u4e3a\u57fa\u56e0\u540d\uff0c\u5217\u540d\u4e3a\u6837\u672c\u4fe1\u606f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">exp<\/span><-read.table(<span class=\"code-snippet__string\">\"EXP.txt\"<\/span>,header=<span class=\"code-snippet__built_in\">T<\/span>,row.names=<span class=\"code-snippet__number\">1<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,check.names=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.27037037037037037\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_ICIF0mtUwDzoZ2jib0rSXib0UnsGfw.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSold3KMQ00F8HlibWDicV0TsqSX4fICIF0mtUwDzoZ2jib0rSXib0UnsGfw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5229\u7528MCPcount R\u5305\u8ba1\u7b97\u514d\u75ab\u6d78\u6da6\u4e30\u5ea6\uff0c\u901a\u8fc7MCPcounter.estimate\u51fd\u6570\u8fdb\u884c\u8ba1\u7b97<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">MCP<- MCPcounter.estimate(<span class=\"code-snippet__built_in\">exp<\/span>, featuresType = <span class=\"code-snippet__string\">\"HUGO_symbols\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fdd\u5b58MCPcounter\u7ed3\u679c\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">write.table(MCP,file=<span class=\"code-snippet__string\">\"MCPcounter.txt\"<\/span>,row.names=<span class=\"code-snippet__built_in\">T<\/span>,col.names=<span class=\"code-snippet__built_in\">T<\/span>,<span class=\"code-snippet__built_in\">quote<\/span>=<span class=\"code-snippet__built_in\">F<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u884c\u5217\u8f6c\u7f6e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">MCP<-as.data.frame(t(MCP))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06\u884c\u540d\u8f6c\u5316\u4e3a\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">MCP<-rownames_to_column(MCP,var=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fde\u63a5\u4e9a\u578b\u5206\u7ec4\u4fe1\u606f\u548c\u514d\u75ab\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">MCPcluster<-left_join(MCP,sub,by=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u957f\u5bbd\u6570\u636e\u8f6c\u6362<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">MCPcluster<-melt(MCPcluster,id.vars=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"Cluster\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(MCPcluster)<-<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"Cluster\"<\/span>,<span class=\"code-snippet__string\">\"celltype\"<\/span>,<span class=\"code-snippet__string\">\"MCPcounter\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u663e\u8457\u5dee\u5f02\u5206\u6790\u5c0f\u63d0\u7434+\u7bb1\u7ebf\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">boxplot_MCPCounter<- ggplot(MCPcluster, aes(x = celltype, y = MCPcounter))+<\/span><\/code><code><span class=\"code-snippet_outer\">labs(y=<span class=\"code-snippet__string\">\"MCPcounter\"<\/span>,x= <span class=\"code-snippet__string\">\"\"<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_boxplot(aes(fill = Cluster),position=position_dodge(<span class=\"code-snippet__number\">0.5<\/span>),width=<span class=\"code-snippet__number\">0.5<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">scale_fill_npg()+<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fee\u6539\u4e3b\u9898<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">theme_classic() +<\/span><\/code><code><span class=\"code-snippet_outer\">theme(axis.title = element_text(size = <span class=\"code-snippet__number\">12<\/span>,color =<span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>,color = <span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid.minor.y = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid.minor.x = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text.x = element_text(angle = <span class=\"code-snippet__number\">45<\/span>, hjust = <span class=\"code-snippet__number\">1<\/span> ),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.position = <span class=\"code-snippet__string\">\"top\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">legend.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.title= element_text(size= <span class=\"code-snippet__number\">12<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">) +<\/span><\/code><code><span class=\"code-snippet_outer\">stat_compare_means(aes(group = Cluster),<\/span><\/code><code><span class=\"code-snippet_outer\">label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">method = <span class=\"code-snippet__string\">\"kruskal.test\"<\/span>,<span class=\"code-snippet__comment\">#kruskal.test\u591a\u7ec4\u68c0\u9a8c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">hide.ns = <span class=\"code-snippet__built_in\">T<\/span>)<span class=\"code-snippet__comment\">#\u9690\u85cf\u4e0d\u663e\u8457\u7684<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(file=<span class=\"code-snippet__string\">\"MCPCounter.pdf\"<\/span>,boxplot_MCPCounter,height=<span class=\"code-snippet__number\">10<\/span>,width=<span class=\"code-snippet__number\">15<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>CIBERSORT\u7b97\u6cd5\u4e2d\u4e0d\u540c\u4e9a\u578b\u4e4b\u95f4\u514d\u75ab\u6d78\u6da6\u663e\u8457\u5dee\u5f02\u5206\u6790<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#22\u79cd\u514d\u75ab\u7ec6\u80de\u8868\u8fbe\u91cf\u6587\u4ef6\uff0c\u7b2c\u4e00\u5217\u4e3aGene symbol,\u5176\u4ed6\u5217\u4e3a22\u79cd\u514d\u75ab\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">LM22.file <- <span class=\"code-snippet__string\">\"LM22.txt\"<\/span><\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.2564814814814815\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_eicibABJGJ5kc7FdxH9PpFDOg803bw.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSJdqkT8EkweActILKHM2yfBx5VibeicibABJGJ5kc7FdxH9PpFDOg803bw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">TCGA_exp.file <- <span class=\"code-snippet__string\">\"EXP.txt\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#perm\u8868\u793a\u7f6e\u6362\u6b21\u6570, QN\u5982\u679c\u662f\u82af\u7247\u8bbe\u7f6e\u4e3aT\uff0c\u5982\u679c\u662f\u6d4b\u5e8f\u5c31\u8bbe\u7f6e\u4e3aF<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">TCGA_cibersort.results <- CIBERSORT(LM22.file ,TCGA_exp.file, perm = <span class=\"code-snippet__number\">50<\/span>, QN = <span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(TCGA_cibersort.results, <span class=\"code-snippet__string\">\"TCGA_CIBERSORT.txt\"<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,col.names=<span class=\"code-snippet__built_in\">T<\/span>,row.names=<span class=\"code-snippet__built_in\">T<\/span>,<span class=\"code-snippet__built_in\">quote<\/span>=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6cibersort\u524d22\u5217\u6570\u636e\uff0c23-25\u5217\u4e3a P-value\uff0c P-value\uff0cRMSE\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort_data <- as.data.frame(TCGA_cibersort.results[,<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">22<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06\u884c\u540d\u8f6c\u5316\u4e3a\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort_data<-rownames_to_column(cibersort_data,var=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fde\u63a5\u4e9a\u578b\u5206\u7ec4\u4fe1\u606f\u548c\u514d\u75ab\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort<-left_join(cibersort_data,sub,by=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u957f\u5bbd\u6570\u636e\u8f6c\u6362<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort<-melt(cibersort,id.vars=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"Cluster\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(cibersort)<-<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"Cluster\"<\/span>,<span class=\"code-snippet__string\">\"celltype\"<\/span>,<span class=\"code-snippet__string\">\"CIBERSORT\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u663e\u8457\u5dee\u5f02\u5c0f\u63d0\u7434+\u7bb1\u7ebf\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">boxplot_CIBERSORT<- ggplot(cibersort, aes(x = celltype, y =CIBERSORT ))+<\/span><\/code><code><span class=\"code-snippet_outer\">labs(y=<span class=\"code-snippet__string\">\"CIBERSORT\"<\/span>,x= <span class=\"code-snippet__string\">\"\"<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_boxplot(aes(fill = Cluster),position=position_dodge(<span class=\"code-snippet__number\">0.5<\/span>),width=<span class=\"code-snippet__number\">0.5<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">scale_fill_npg()+<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fee\u6539\u4e3b\u9898<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">theme_classic() +<\/span><\/code><code><span class=\"code-snippet_outer\">theme(axis.title = element_text(size = <span class=\"code-snippet__number\">12<\/span>,color =<span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>,color = <span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid.minor.y = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid.minor.x = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text.x = element_text(angle = <span class=\"code-snippet__number\">45<\/span>, hjust = <span class=\"code-snippet__number\">1<\/span> ),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.position = <span class=\"code-snippet__string\">\"top\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">legend.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.title= element_text(size= <span class=\"code-snippet__number\">12<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">) +<\/span><\/code><code><span class=\"code-snippet_outer\">stat_compare_means(aes(group = Cluster),<\/span><\/code><code><span class=\"code-snippet_outer\">label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>,<span class=\"code-snippet__comment\">#\u6dfb\u52a0\u661f\u53f7\u6807\u7b7e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">method = <span class=\"code-snippet__string\">\"kruskal.test\"<\/span>,<span class=\"code-snippet__comment\">#kruskal.test\u591a\u7ec4\u68c0\u9a8c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">hide.ns = <span class=\"code-snippet__built_in\">F<\/span>)<span class=\"code-snippet__comment\">#\u663e\u793a\u4e0d\u663e\u8457\u7684<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fdd\u5b58\u56fe\u7247<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(file=<span class=\"code-snippet__string\">\"CIBERSORT.pdf\"<\/span>,boxplot_CIBERSORT,height=<span class=\"code-snippet__number\">10<\/span>,width=<span class=\"code-snippet__number\">15<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;\" powered-by=\"xiumi.us\">\n<p><strong>\u7ed3\u679c\u6587\u4ef6<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"color: rgb(16, 116, 231);padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>1.MCPCounter.pdf<\/strong><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u8be5\u7ed3\u679c\u56fe\u7247\u4e3a MCPCounter\u7b97\u6cd5\u4e2d\u4e0d\u540c\u4e9a\u578b\u4e4b\u95f4\u5177\u6709\u663e\u8457\u5dee\u5f02\u7684\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u6bd4\u8f83\u56fe\uff0c1\u8868\u793ap\u5c0f\u4e8e0.05\uff0c11\u8868\u793ap\u5c0f\u4e8e0.01\uff0c111\u8868\u793ap\u5c0f\u4e8e0.001<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6675925925925926\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_Kt532tibxnb7Mmcr4zI0MF7MwdOWag.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSsHI1tluTQAibPgbxlnqvzoBuC1Kt532tibxnb7Mmcr4zI0MF7MwdOWag\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong style=\"color: rgb(16, 116, 231);\">2.CIBERSORT.pdf<\/strong><br \/><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u8be5\u7ed3\u679c\u56fe\u7247\u4e3a CIBERSORT\u7b97\u6cd5\u4e2d\u4e0d\u540c\u4e9a\u578b\u4e4b\u95f4\u5177\u6709\u663e\u8457\u5dee\u5f02\u7684\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u6bd4\u8f83\u56fe\uff0c1\u8868\u793ap\u5c0f\u4e8e0.05\uff0c11\u8868\u793ap\u5c0f\u4e8e0.01\uff0c111\u8868\u793ap\u5c0f\u4e8e0.001<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6685185185185185\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_olUTiaAnYU0CM9qxAZJNsia7ffjkfA.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSZcmWgAndI2IIEtlc9iaP4kH8Su6olUTiaAnYU0CM9qxAZJNsia7ffjkfA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>3.MCPcounter.txt<\/strong><\/span><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u8be5\u7ed3\u679c\u6587\u4ef6\u4e3a\u5229\u7528MCPcounter\u7b97\u6cd5\u8ba1\u7b97\u7684\u514d\u75ab\u7ec6\u80de\u77e9\u9635\uff0c\u884c\u540d\u4e3a\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\uff0c\u5217\u540d\u4e3a\u6837\u672c\u540d\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.15555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_nx9Ff4kLK30HPVMPvib7e8eP2cyVbQ.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSLmXODgVQ3zfau0BtApTsLSGaHnx9Ff4kLK30HPVMPvib7e8eP2cyVbQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"color: rgb(16, 116, 231);\">4.TCGA_CIBERSORT.txt<\/span><\/strong><\/p>\n<p>\u8be5\u7ed3\u679c\u6587\u4ef6\u4e3a\u5229\u7528CIBERSORT\u7b97\u6cd5\u8ba1\u7b97\u7684\u514d\u75ab\u7ec6\u80de\u77e9\u9635\uff0c\u5217\u540d\u4e3a\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\uff0c\u884c\u540d\u4e3a\u6837\u672c\u540d\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.25925925925925924\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_q304ER0ohQY9meUUk23hXz0MtGV6xw.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzS6KT68EibbEW123zvyvDnFfIyPq304ER0ohQY9meUUk23hXz0MtGV6xw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"color: rgb(16, 116, 231);padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><strong>5.K3.txt<\/strong><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u8be5\u7ed3\u679c\u6587\u4ef6\u4e3a\u5229\u7528ConsensusCluster\u4e00\u81f4\u6027\u805a\u7c7b\u5206\u6790\uff0cK=3\u65f6\u4e9a\u578b\u5206\u7ec4\u4fe1\u606f\u6587\u4ef6,\u7b2c\u4e00\u5217\u4e3a\u6837\u672c\u540d\uff0c\u7b2c\u4e8c\u5217\u4e3a\u5206\u7ec4\u4fe1\u606f\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.2916666666666667\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_vkdcSpKXWobDvQNsYzzg6ibW8I3iaA.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSuC6UBJ1KFABxtcwvohwFBlhrvNvkdcSpKXWobDvQNsYzzg6ibW8I3iaA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<p>\u6700\u7ec8\u5c0f\u82b1\u6210\u529f\u7684\u5bf9\u4e0d\u540c\u4e9a\u578b\u901a\u8fc7CIBERSORT\u548cMCPcounter\u7b97\u6cd5\u8fdb\u884c\u4e86\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\uff0c\u770b\u8d77\u6765\u56fe\u7247\u6548\u679c\u975e\u5e38\u4e0d\u9519\uff0c\u6b22\u8fce\u5927\u5bb6\u548c\u5c0f\u82b1\u4e00\u8d77\u8ba8\u8bba\u5b66\u4e60\u5440\uff01\u514d\u75ab\u6d78\u6da6\u76f8\u5173\u5206\u6790\u5185\u5bb9\uff0c\u4f8b\u5982\u5355\u6837\u672c\u5bcc\u96c6\u7b97\u6cd5\u5206\u6790\u514d\u75ab\u6d78\u6da6\u4e30\u5ea6(http:\/\/www.biocloudservice.com\/106\/106.php)\uff0c\u8ba1\u7b9764\u79cd\u514d\u75ab\u7ec6\u80de\u76f8\u5bf9\u542b\u91cf(http:\/\/www.biocloudservice.com\/107\/107.php)\u7b49\u90fd\u53ef\u4ee5\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u4e91\u5e73\u53f0\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\uff0c\u4e00\u952e\u5b8c\u6210\u8be5\u5206\u6790\u55f7\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u5c1d\u8bd5\u5965\uff0c\u7f51\u5740\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002\u4eca\u5929\u5c0f\u82b1\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\uff0c\u4e0b\u671f\u518d\u89c1\u5965\u3002<\/p>\n<p><br \/><\/p>\n<p>\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1 – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/p>\n<p><br \/><\/p>\n<p>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a18502195490\uff08\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/p>\n<\/section>\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row;transform: translate3d(40px, 0px, 0px);margin-bottom: -40px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 26px;height: 26px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);border-left: 1px solid rgb(160, 160, 160);border-bottom-left-radius: 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_pBN4r4MaBCk9p9v16yZwvMvS5nM7sQ-9.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"895\" style=\"\" data-recalc-dims=\"1\" \/><\/span><\/a><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3488888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_wGGHoIUWPWSIgBgzuWA7zggZhslM2g-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSbGdkTKlXI3tbocicP8GUCzibJV5ibjQs6D7lQhs1pnDYZQiauBwGGHoIUWPWSIgBgzuWA7zggZhslM2g\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p powered-by=\"xiumi.us\">\uff08\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8df3\u8f6c\uff09<\/p>\n<section style=\"text-align: left;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;min-width: 10%;vertical-align: top;padding-right: 20px;\">\n<section style=\"transform: translate3d(20px, 0px, 0px);margin: 10px 0% -30px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);text-align: justify;font-size: 12px;\">\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.866\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_hTb9TCBmE3ZicYpP2jqDjWX6xDRuTQ.gif?resize=18%2C15\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_gif\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSj0ZoyOLjZBYjCtRku4tPJdKviahTb9TCBmE3ZicYpP2jqDjWX6xDRuTQ\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"500\" height=\"15\" style=\"width: 18px;height: 15px;\" width=\"18\" data-recalc-dims=\"1\" \/> \u70b9\u4e00\u4e0b\u9605\u8bfb\u539f\u6587\u4e86\u89e3\u66f4\u591a\u8d44\u8baf<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 50px;height: 40px;vertical-align: top;overflow: hidden;\">\n<section style=\"text-align: center;margin-right: 0%;margin-left: 0%;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7709090909090909\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_QM3WCXd8qvGSWQt39mNFgcFB8x6CfA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSL2jMD7ichFZu3oAMIdicF0lCfyQM3WCXd8qvGSWQt39mNFgcFB8x6CfA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"275\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u751f\u4fe1\u6587\u7ae0\u5fc5\u5907\u5206\u6790\u5185\u5bb9\uff0c\u5bf9\u4e9a\u578b\u5229\u7528MCPcounter\u548cCIBERSORT\u4e24\u79cd\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790 \u5c0f\u82b1&nbs […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57820"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=57820"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57820\/revisions"}],"predecessor-version":[{"id":57832,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57820\/revisions\/57832"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=57820"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=57820"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=57820"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

{"id":57820,"date":"2024-06-16T20:36:22","date_gmt":"2024-06-16T12:36:22","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=57820"},"modified":"2024-06-16T20:36:22","modified_gmt":"2024-06-16T12:36:22","slug":"%e7%94%9f%e4%bf%a1%e6%96%87%e7%ab%a0%e5%bf%85%e5%a4%87%e5%88%86%e6%9e%90%e5%86%85%e5%ae%b9%ef%bc%8c%e5%af%b9%e4%ba%9a%e5%9e%8b%e5%88%a9%e7%94%a8mcpcounter%e5%92%8ccibersort%e4%b8%a4%e7%a7%8d%e7%ae%97","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=57820","title":{"rendered":"\u751f\u4fe1\u6587\u7ae0\u5fc5\u5907\u5206\u6790\u5185\u5bb9\uff0c\u5bf9\u4e9a\u578b\u5229\u7528MCPcounter\u548cCIBERSORT\u4e24\u79cd\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790"},"content":{"rendered":"

\n
\n<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner,body{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media{padding:20px;overflow:hidden;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}@media screen and (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media_content{overflow:hidden;color:#333;font-size:17px;word-wrap:break-word;-webkit-hyphens:auto;-ms-hyphens:auto;hyphens:auto;text-align:justify;position:relative;z-index:0}.rich_media_content *{max-width:100%!important;box-sizing:border-box!important;-webkit-box-sizing:border-box!important;word-wrap:break-word!important}.rich_media_content p{clear:both;min-height:1em}.rich_media_content em{font-style:italic}.rich_media_content fieldset{min-width:0}.rich_media_content .list-paddingleft-1,.rich_media_content .list-paddingleft-2,.rich_media_content .list-paddingleft-3{padding-left:2.2em}.rich_media_content .list-paddingleft-1 .list-paddingleft-2,.rich_media_content .list-paddingleft-2 .list-paddingleft-2,.rich_media_content .list-paddingleft-3 .list-paddingleft-2{padding-left:30px}.rich_media_content .list-paddingleft-1{padding-left:1.2em}.rich_media_content .list-paddingleft-3{padding-left:3.2em}.rich_media_content .code-snippet,.rich_media_content .code-snippet__fix{max-width:1000%!important}.rich_media_content .code-snippet *,.rich_media_content .code-snippet__fix *{max-width:1000%!important}.rich_media_title{font-size:22px;line-height:1.4;margin-bottom:13px;padding-bottom:13px;border-bottom:1px solid #e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag .code-snippet__attr{color:#0e9ce5}.code-snippet__string,.code-snippet__doctag{color:#d14}.code-snippet__title,.code-snippet__section,.code-snippet__selector-id{color:#d14}.code-snippet__subst{font-weight:normal}.code-snippet__type,.code-snippet__class .code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u751f\u4fe1\u6587\u7ae0\u5fc5\u5907\u5206\u6790\u5185\u5bb9\uff0c\u5bf9\u4e9a\u578b\u5229\u7528MCPcounter\u548cCIBERSORT\u4e24\u79cd\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790\",\"time\":\"2023-08-21 19:02:53\",\"timeStamp\":\"1692615773\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491566&idx=5&sn=852ad8c59fcfe0b38977532fd727c462&chksm=cf7c8f2bf80b063df6dbe4849a99497b8edf7232f7f1508e0a110a922b60d7f8cb76ba3adc6e#rd\" target=\"_blank\" rel=\"noopener\">\u751f\u4fe1\u6587\u7ae0\u5fc5\u5907\u5206\u6790\u5185\u5bb9\uff0c\u5bf9\u4e9a\u578b\u5229\u7528MCPcounter\u548cCIBERSORT\u4e24\u79cd\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u82b1 <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-08-21 19:02:53<\/em><\/div>\n<p><content><\/p>\n<div class=\"reprint\">\n<p>\u8f6c\u81ea\u516c\u4f17\u53f7\uff1a\u751f\u4fe1\u82b1<br \/><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484214&idx=1&sn=9a5856b790d928fbaa6657c4c6450175\" target=\"_blank\" title=\"\u9605\u8bfb\u539f\u6587\" rel=\"noopener\">http:\/\/mp.weixin.qq.com\/s?__biz=MzkzODUxNjcwMA==&mid=2247484214&idx=1&sn=9a5856b790d928fbaa6657c4c6450175<\/a><\/p>\n<\/div>\n<section style=\"font-size: 14px;margin-bottom: 24px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(65, 128, 216);\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;padding-left: 12px;\">\n<section style=\"text-align: justify;color: rgb(255, 255, 255);\" powered-by=\"xiumi.us\">\n<p><strong>\u70b9\u51fb\u84dd\u5b57 \u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;margin-right: -17px;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(201, 229, 70);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 34px;align-self: flex-start;flex: 0 0 auto;height: auto;overflow: hidden;\">\n<section style=\"transform: rotateZ(60deg);\" powered-by=\"xiumi.us\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;\">\n<section style=\"width: 100%;height: 5px;background-color: rgb(255, 255, 255);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u4eca\u5929\u5c0f\u82b1\u5e26\u6765\u7684\u5206\u4eab\u4e3a\u9996\u5148\u5229\u7528ConsensusCluster\u5bf9\u80bf\u7624\u6837\u672c\u8fdb\u884c\u5206\u578b\uff0c\u7136\u540e\u5bf9\u4e0d\u540c\u4e9a\u578b\u5229\u7528MCPcounter\u548cCIBERSORT\u4e24\u79cd\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\u6700\u540e\u5bf9\u4e0d\u540c\u4e9a\u578b\u4e4b\u95f4\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u8fdb\u884c\u663e\u8457\u5dee\u5f02\u5206\u6790\uff0c\u8be5\u5206\u6790\u5728\u751f\u4fe1\u6587\u7ae0\u4e2d\u51fa\u73b0\u7684\u9891\u7387\u975e\u5e38\u9ad8\uff0c\u503c\u5f97\u5c0f\u4f19\u4f34\u4eec\u5b66\u4e60\u55f7\uff0c\u63a5\u4e0b\u6765\u8ddf\u7740\u5c0f\u82b1\u5f00\u542f\u4eca\u5929\u7684\u5b66\u4e60\u4e4b\u65c5\u5427\uff01<br \/><\/p>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;\" powered-by=\"xiumi.us\">\n<p><strong>\u51c6\u5907\u9700\u8981\u7684R\u5305<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5b89\u88c5\u9700\u8981\u7684R\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"tidyverse\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"devtools\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">library(devtools)<\/span><\/code><code><span class=\"code-snippet_outer\">install_github(<span class=\"code-snippet__string\">\"ebecht\/MCPcounter\"<\/span>,ref=<span class=\"code-snippet__string\">\"master\"<\/span>, subdir=<span class=\"code-snippet__string\">\"Source\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggplot2\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">install.packages(<span class=\"code-snippet__string\">\"ggsci\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">BiocManager::install(<span class=\"code-snippet__string\">\"ConsensusClusterPlus\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u52a0\u8f7d\u9700\u8981\u7684R\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(MCPcounter)<\/span><\/code><code><span class=\"code-snippet_outer\">library(reshape2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(tidyverse)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggsci)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ConsensusClusterPlus)<\/span><\/code><code><span class=\"code-snippet_outer\">source(<span class=\"code-snippet__string\">'Cibersort.R'<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;\" powered-by=\"xiumi.us\">\n<p><strong>ConsensusCluster\u4e00\u81f4\u6027\u805a\u7c7b\u5206\u6790<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7b5b\u9009\u51fa\u7684\u6709\u9884\u540e\u7684TEX\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6587\u4ef6\uff0c\u884c\u540d\u4e3a\u57fa\u56e0\u540d\uff0c\u5217\u4e3a\u6837\u672c\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">expr1<- read.table(file = <span class=\"code-snippet__string\">\"expr_cluster.txt\"<\/span>,<span class=\"code-snippet__comment\">#\u5de5\u4f5c\u76ee\u5f55\u4e0b\u7684\u6587\u4ef6\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">header = <span class=\"code-snippet__built_in\">T<\/span>,<span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u6709\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sep = <span class=\"code-snippet__string\">\"t\"<\/span>,<span class=\"code-snippet__comment\">#\u5206\u9694\u7b26<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">row.names = <span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u884c\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">check.names=<span class=\"code-snippet__built_in\">F<\/span>)<span class=\"code-snippet__comment\">#\u4e0d\u68c0\u67e5\u884c\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dir.create(<span class=\"code-snippet__string\">'ConsensusCluster\/'<\/span>)<span class=\"code-snippet__comment\">#\u6784\u5efa\u6587\u4ef6\u5939<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#ConsensusCluster\u4e00\u81f4\u6027\u805a\u7c7b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">results = ConsensusClusterPlus(as.matrix(expr1),<\/span><\/code><code><span class=\"code-snippet_outer\">maxK=<span class=\"code-snippet__number\">6<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">reps=<span class=\"code-snippet__number\">100<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">pItem=<span class=\"code-snippet__number\">0.8<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">pFeature=<span class=\"code-snippet__number\">1<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">title=<span class=\"code-snippet__string\">'ConsensusCluster\/'<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">clusterAlg=<span class=\"code-snippet__string\">\"km\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">distance=<span class=\"code-snippet__string\">\"euclidean\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">seed=<span class=\"code-snippet__number\">123456<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">plot=<span class=\"code-snippet__string\">\"pdf\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">icl <- calcICL(results,title = <span class=\"code-snippet__string\">'ConsensusCluster\/'<\/span>,plot = <span class=\"code-snippet__string\">'pdf'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">Kvec = <span class=\"code-snippet__number\">2<\/span>:<span class=\"code-snippet__number\">6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">x1 = <span class=\"code-snippet__number\">0.1<\/span>; x2 = <span class=\"code-snippet__number\">0.6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">PAC = <span class=\"code-snippet__built_in\">rep<\/span>(<span class=\"code-snippet__literal\">NA<\/span>,<span class=\"code-snippet__built_in\">length<\/span>(Kvec))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">names<\/span>(PAC) = paste(<span class=\"code-snippet__string\">\"K=\"<\/span>,Kvec,sep=<span class=\"code-snippet__string\">\"\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> Kvec){<\/span><\/code><code><span class=\"code-snippet_outer\">M = results[[i]]$consensusMatrix<\/span><\/code><code><span class=\"code-snippet_outer\">Fn = ecdf(M[lower.tri(M)])<\/span><\/code><code><span class=\"code-snippet_outer\">PAC[i-<span class=\"code-snippet__number\">1<\/span>] = Fn(x2) - Fn(x1)<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">optK = Kvec[which.min(PAC)]<\/span><\/code><code><span class=\"code-snippet_outer\">optK<\/span><\/code><code><span class=\"code-snippet_outer\">PAC <- as.data.frame(PAC)<\/span><\/code><code><span class=\"code-snippet_outer\">PAC$K <- 2:<span class=\"code-snippet__number\">6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236PAC\u9a8c\u8bc1\u66f2\u7ebf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggplot(PAC,aes(factor(K),PAC,group=<span class=\"code-snippet__number\">1<\/span>))+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_line()+<\/span><\/code><code><span class=\"code-snippet_outer\">theme_bw()+theme(panel.grid = element_blank())+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_point(size=<span class=\"code-snippet__number\">4<\/span>,shape=<span class=\"code-snippet__number\">21<\/span>,color=<span class=\"code-snippet__string\">'darkred'<\/span>,fill=<span class=\"code-snippet__string\">'skyblue'<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">ylab(<span class=\"code-snippet__string\">'Proportion of ambiguous clustering'<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">xlab(<span class=\"code-snippet__string\">'Cluster number K'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(<span class=\"code-snippet__string\">\"PAC.pdf\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6700\u5c0f\u503c\u4e5f\u5c31\u662f\u6700\u4f73K\uff0c\u4ecd\u7136\u4e3a3.<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6\u5206\u578b\u4fe1\u606f\uff0ck=3<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">clusterNum=<span class=\"code-snippet__number\">3<\/span> <span class=\"code-snippet__comment\">#k\u503c\u53d8\u5316\u8c03\u6574<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cluster=results[[clusterNum]][[<span class=\"code-snippet__string\">\"consensusClass\"<\/span>]]<\/span><\/code><code><span class=\"code-snippet_outer\">sub <- data.frame(Sample=<span class=\"code-snippet__built_in\">names<\/span>(cluster),Cluster=cluster)<\/span><\/code><code><span class=\"code-snippet_outer\">sub$Cluster <- paste0(<span class=\"code-snippet__string\">'C'<\/span>,sub$Cluster)<\/span><\/code><code><span class=\"code-snippet_outer\">table(sub$Cluster)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(sub,file = <span class=\"code-snippet__string\">\"K3.txt\"<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,<span class=\"code-snippet__built_in\">quote<\/span>=<span class=\"code-snippet__built_in\">F<\/span>)<span class=\"code-snippet__comment\">#\u8f93\u51fa\u7ed3\u679c<\/span><\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 12px;\" powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 11px;\"><strong>\u4e9a\u578b\u95f4\u5229\u7528MCPCounter\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u663e\u8457\u5dee\u5f02\u5206\u6790<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#EXP.txt\uff0c\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6587\u4ef6\uff0c\u884c\u540d\u4e3a\u57fa\u56e0\u540d\uff0c\u5217\u540d\u4e3a\u6837\u672c\u4fe1\u606f\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">exp<\/span><-read.table(<span class=\"code-snippet__string\">\"EXP.txt\"<\/span>,header=<span class=\"code-snippet__built_in\">T<\/span>,row.names=<span class=\"code-snippet__number\">1<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,check.names=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.27037037037037037\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_ICIF0mtUwDzoZ2jib0rSXib0UnsGfw.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSold3KMQ00F8HlibWDicV0TsqSX4fICIF0mtUwDzoZ2jib0rSXib0UnsGfw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5229\u7528MCPcount R\u5305\u8ba1\u7b97\u514d\u75ab\u6d78\u6da6\u4e30\u5ea6\uff0c\u901a\u8fc7MCPcounter.estimate\u51fd\u6570\u8fdb\u884c\u8ba1\u7b97<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">MCP<- MCPcounter.estimate(<span class=\"code-snippet__built_in\">exp<\/span>, featuresType = <span class=\"code-snippet__string\">\"HUGO_symbols\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fdd\u5b58MCPcounter\u7ed3\u679c\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">write.table(MCP,file=<span class=\"code-snippet__string\">\"MCPcounter.txt\"<\/span>,row.names=<span class=\"code-snippet__built_in\">T<\/span>,col.names=<span class=\"code-snippet__built_in\">T<\/span>,<span class=\"code-snippet__built_in\">quote<\/span>=<span class=\"code-snippet__built_in\">F<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u884c\u5217\u8f6c\u7f6e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">MCP<-as.data.frame(t(MCP))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06\u884c\u540d\u8f6c\u5316\u4e3a\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">MCP<-rownames_to_column(MCP,var=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fde\u63a5\u4e9a\u578b\u5206\u7ec4\u4fe1\u606f\u548c\u514d\u75ab\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">MCPcluster<-left_join(MCP,sub,by=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u957f\u5bbd\u6570\u636e\u8f6c\u6362<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">MCPcluster<-melt(MCPcluster,id.vars=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"Cluster\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(MCPcluster)<-<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"Cluster\"<\/span>,<span class=\"code-snippet__string\">\"celltype\"<\/span>,<span class=\"code-snippet__string\">\"MCPcounter\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u663e\u8457\u5dee\u5f02\u5206\u6790\u5c0f\u63d0\u7434+\u7bb1\u7ebf\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">boxplot_MCPCounter<- ggplot(MCPcluster, aes(x = celltype, y = MCPcounter))+<\/span><\/code><code><span class=\"code-snippet_outer\">labs(y=<span class=\"code-snippet__string\">\"MCPcounter\"<\/span>,x= <span class=\"code-snippet__string\">\"\"<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_boxplot(aes(fill = Cluster),position=position_dodge(<span class=\"code-snippet__number\">0.5<\/span>),width=<span class=\"code-snippet__number\">0.5<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">scale_fill_npg()+<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fee\u6539\u4e3b\u9898<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">theme_classic() +<\/span><\/code><code><span class=\"code-snippet_outer\">theme(axis.title = element_text(size = <span class=\"code-snippet__number\">12<\/span>,color =<span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>,color = <span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid.minor.y = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid.minor.x = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text.x = element_text(angle = <span class=\"code-snippet__number\">45<\/span>, hjust = <span class=\"code-snippet__number\">1<\/span> ),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.position = <span class=\"code-snippet__string\">\"top\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">legend.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.title= element_text(size= <span class=\"code-snippet__number\">12<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">) +<\/span><\/code><code><span class=\"code-snippet_outer\">stat_compare_means(aes(group = Cluster),<\/span><\/code><code><span class=\"code-snippet_outer\">label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">method = <span class=\"code-snippet__string\">\"kruskal.test\"<\/span>,<span class=\"code-snippet__comment\">#kruskal.test\u591a\u7ec4\u68c0\u9a8c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">hide.ns = <span class=\"code-snippet__built_in\">T<\/span>)<span class=\"code-snippet__comment\">#\u9690\u85cf\u4e0d\u663e\u8457\u7684<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(file=<span class=\"code-snippet__string\">\"MCPCounter.pdf\"<\/span>,boxplot_MCPCounter,height=<span class=\"code-snippet__number\">10<\/span>,width=<span class=\"code-snippet__number\">15<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>CIBERSORT\u7b97\u6cd5\u4e2d\u4e0d\u540c\u4e9a\u578b\u4e4b\u95f4\u514d\u75ab\u6d78\u6da6\u663e\u8457\u5dee\u5f02\u5206\u6790<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#22\u79cd\u514d\u75ab\u7ec6\u80de\u8868\u8fbe\u91cf\u6587\u4ef6\uff0c\u7b2c\u4e00\u5217\u4e3aGene symbol,\u5176\u4ed6\u5217\u4e3a22\u79cd\u514d\u75ab\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">LM22.file <- <span class=\"code-snippet__string\">\"LM22.txt\"<\/span><\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.2564814814814815\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_eicibABJGJ5kc7FdxH9PpFDOg803bw.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSJdqkT8EkweActILKHM2yfBx5VibeicibABJGJ5kc7FdxH9PpFDOg803bw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">TCGA_exp.file <- <span class=\"code-snippet__string\">\"EXP.txt\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#perm\u8868\u793a\u7f6e\u6362\u6b21\u6570, QN\u5982\u679c\u662f\u82af\u7247\u8bbe\u7f6e\u4e3aT\uff0c\u5982\u679c\u662f\u6d4b\u5e8f\u5c31\u8bbe\u7f6e\u4e3aF<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">TCGA_cibersort.results <- CIBERSORT(LM22.file ,TCGA_exp.file, perm = <span class=\"code-snippet__number\">50<\/span>, QN = <span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(TCGA_cibersort.results, <span class=\"code-snippet__string\">\"TCGA_CIBERSORT.txt\"<\/span>,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,col.names=<span class=\"code-snippet__built_in\">T<\/span>,row.names=<span class=\"code-snippet__built_in\">T<\/span>,<span class=\"code-snippet__built_in\">quote<\/span>=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6cibersort\u524d22\u5217\u6570\u636e\uff0c23-25\u5217\u4e3a P-value\uff0c P-value\uff0cRMSE\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort_data <- as.data.frame(TCGA_cibersort.results[,<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">22<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06\u884c\u540d\u8f6c\u5316\u4e3a\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort_data<-rownames_to_column(cibersort_data,var=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fde\u63a5\u4e9a\u578b\u5206\u7ec4\u4fe1\u606f\u548c\u514d\u75ab\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort<-left_join(cibersort_data,sub,by=<span class=\"code-snippet__string\">\"Sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u957f\u5bbd\u6570\u636e\u8f6c\u6362<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cibersort<-melt(cibersort,id.vars=<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"Cluster\"<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u5217\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(cibersort)<-<span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"Sample\"<\/span>,<span class=\"code-snippet__string\">\"Cluster\"<\/span>,<span class=\"code-snippet__string\">\"celltype\"<\/span>,<span class=\"code-snippet__string\">\"CIBERSORT\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u663e\u8457\u5dee\u5f02\u5c0f\u63d0\u7434+\u7bb1\u7ebf\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">boxplot_CIBERSORT<- ggplot(cibersort, aes(x = celltype, y =CIBERSORT ))+<\/span><\/code><code><span class=\"code-snippet_outer\">labs(y=<span class=\"code-snippet__string\">\"CIBERSORT\"<\/span>,x= <span class=\"code-snippet__string\">\"\"<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">geom_boxplot(aes(fill = Cluster),position=position_dodge(<span class=\"code-snippet__number\">0.5<\/span>),width=<span class=\"code-snippet__number\">0.5<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\">scale_fill_npg()+<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fee\u6539\u4e3b\u9898<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">theme_classic() +<\/span><\/code><code><span class=\"code-snippet_outer\">theme(axis.title = element_text(size = <span class=\"code-snippet__number\">12<\/span>,color =<span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>,color = <span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid.minor.y = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid.minor.x = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">axis.text.x = element_text(angle = <span class=\"code-snippet__number\">45<\/span>, hjust = <span class=\"code-snippet__number\">1<\/span> ),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid=element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.position = <span class=\"code-snippet__string\">\"top\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\">legend.text = element_text(size= <span class=\"code-snippet__number\">12<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">legend.title= element_text(size= <span class=\"code-snippet__number\">12<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">) +<\/span><\/code><code><span class=\"code-snippet_outer\">stat_compare_means(aes(group = Cluster),<\/span><\/code><code><span class=\"code-snippet_outer\">label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>,<span class=\"code-snippet__comment\">#\u6dfb\u52a0\u661f\u53f7\u6807\u7b7e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">method = <span class=\"code-snippet__string\">\"kruskal.test\"<\/span>,<span class=\"code-snippet__comment\">#kruskal.test\u591a\u7ec4\u68c0\u9a8c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">hide.ns = <span class=\"code-snippet__built_in\">F<\/span>)<span class=\"code-snippet__comment\">#\u663e\u793a\u4e0d\u663e\u8457\u7684<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fdd\u5b58\u56fe\u7247<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(file=<span class=\"code-snippet__string\">\"CIBERSORT.pdf\"<\/span>,boxplot_CIBERSORT,height=<span class=\"code-snippet__number\">10<\/span>,width=<span class=\"code-snippet__number\">15<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;transform: translate3d(-15px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;background-color: rgb(129, 167, 211);padding: 13px;\">\n<section style=\"text-align: justify;color: rgb(19, 53, 102);font-size: 16px;\" powered-by=\"xiumi.us\">\n<p><strong>\u7ed3\u679c\u6587\u4ef6<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"color: rgb(16, 116, 231);padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong>1.MCPCounter.pdf<\/strong><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u8be5\u7ed3\u679c\u56fe\u7247\u4e3a MCPCounter\u7b97\u6cd5\u4e2d\u4e0d\u540c\u4e9a\u578b\u4e4b\u95f4\u5177\u6709\u663e\u8457\u5dee\u5f02\u7684\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u6bd4\u8f83\u56fe\uff0c1\u8868\u793ap\u5c0f\u4e8e0.05\uff0c11\u8868\u793ap\u5c0f\u4e8e0.01\uff0c111\u8868\u793ap\u5c0f\u4e8e0.001<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6675925925925926\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_Kt532tibxnb7Mmcr4zI0MF7MwdOWag.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSsHI1tluTQAibPgbxlnqvzoBuC1Kt532tibxnb7Mmcr4zI0MF7MwdOWag\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong style=\"color: rgb(16, 116, 231);\">2.CIBERSORT.pdf<\/strong><br \/><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u8be5\u7ed3\u679c\u56fe\u7247\u4e3a CIBERSORT\u7b97\u6cd5\u4e2d\u4e0d\u540c\u4e9a\u578b\u4e4b\u95f4\u5177\u6709\u663e\u8457\u5dee\u5f02\u7684\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u6bd4\u8f83\u56fe\uff0c1\u8868\u793ap\u5c0f\u4e8e0.05\uff0c11\u8868\u793ap\u5c0f\u4e8e0.01\uff0c111\u8868\u793ap\u5c0f\u4e8e0.001<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6685185185185185\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_olUTiaAnYU0CM9qxAZJNsia7ffjkfA.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSZcmWgAndI2IIEtlc9iaP4kH8Su6olUTiaAnYU0CM9qxAZJNsia7ffjkfA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><span style=\"color: rgb(16, 116, 231);\"><strong>3.MCPcounter.txt<\/strong><\/span><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u8be5\u7ed3\u679c\u6587\u4ef6\u4e3a\u5229\u7528MCPcounter\u7b97\u6cd5\u8ba1\u7b97\u7684\u514d\u75ab\u7ec6\u80de\u77e9\u9635\uff0c\u884c\u540d\u4e3a\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\uff0c\u5217\u540d\u4e3a\u6837\u672c\u540d\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.15555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_nx9Ff4kLK30HPVMPvib7e8eP2cyVbQ.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSLmXODgVQ3zfau0BtApTsLSGaHnx9Ff4kLK30HPVMPvib7e8eP2cyVbQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"color: rgb(16, 116, 231);\">4.TCGA_CIBERSORT.txt<\/span><\/strong><\/p>\n<p>\u8be5\u7ed3\u679c\u6587\u4ef6\u4e3a\u5229\u7528CIBERSORT\u7b97\u6cd5\u8ba1\u7b97\u7684\u514d\u75ab\u7ec6\u80de\u77e9\u9635\uff0c\u5217\u540d\u4e3a\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\uff0c\u884c\u540d\u4e3a\u6837\u672c\u540d\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.25925925925925924\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_q304ER0ohQY9meUUk23hXz0MtGV6xw.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzS6KT68EibbEW123zvyvDnFfIyPq304ER0ohQY9meUUk23hXz0MtGV6xw\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"color: rgb(16, 116, 231);padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><strong>5.K3.txt<\/strong><\/p>\n<\/section>\n<section style=\"padding-right: 12px;padding-left: 12px;\" powered-by=\"xiumi.us\">\n<p>\u8be5\u7ed3\u679c\u6587\u4ef6\u4e3a\u5229\u7528ConsensusCluster\u4e00\u81f4\u6027\u805a\u7c7b\u5206\u6790\uff0cK=3\u65f6\u4e9a\u578b\u5206\u7ec4\u4fe1\u606f\u6587\u4ef6,\u7b2c\u4e00\u5217\u4e3a\u6837\u672c\u540d\uff0c\u7b2c\u4e8c\u5217\u4e3a\u5206\u7ec4\u4fe1\u606f\u3002<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.2916666666666667\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_vkdcSpKXWobDvQNsYzzg6ibW8I3iaA.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSuC6UBJ1KFABxtcwvohwFBlhrvNvkdcSpKXWobDvQNsYzzg6ibW8I3iaA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 98%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;border-style: solid;border-width: 2px;border-color: rgb(160, 160, 160);padding: 27px;\">\n<section style=\"text-align: justify;line-height: 1.6;letter-spacing: 0px;padding-right: 6px;padding-left: 6px;\" powered-by=\"xiumi.us\">\n<p>\u6700\u7ec8\u5c0f\u82b1\u6210\u529f\u7684\u5bf9\u4e0d\u540c\u4e9a\u578b\u901a\u8fc7CIBERSORT\u548cMCPcounter\u7b97\u6cd5\u8fdb\u884c\u4e86\u514d\u75ab\u6d78\u6da6\u5206\u6790\uff0c\uff0c\u770b\u8d77\u6765\u56fe\u7247\u6548\u679c\u975e\u5e38\u4e0d\u9519\uff0c\u6b22\u8fce\u5927\u5bb6\u548c\u5c0f\u82b1\u4e00\u8d77\u8ba8\u8bba\u5b66\u4e60\u5440\uff01\u514d\u75ab\u6d78\u6da6\u76f8\u5173\u5206\u6790\u5185\u5bb9\uff0c\u4f8b\u5982\u5355\u6837\u672c\u5bcc\u96c6\u7b97\u6cd5\u5206\u6790\u514d\u75ab\u6d78\u6da6\u4e30\u5ea6(http:\/\/www.biocloudservice.com\/106\/106.php)\uff0c\u8ba1\u7b9764\u79cd\u514d\u75ab\u7ec6\u80de\u76f8\u5bf9\u542b\u91cf(http:\/\/www.biocloudservice.com\/107\/107.php)\u7b49\u90fd\u53ef\u4ee5\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u96f6\u4ee3\u7801\u4e91\u5e73\u53f0\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\uff0c\u4e00\u952e\u5b8c\u6210\u8be5\u5206\u6790\u55f7\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u5c1d\u8bd5\u5965\uff0c\u7f51\u5740\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002\u4eca\u5929\u5c0f\u82b1\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\uff0c\u4e0b\u671f\u518d\u89c1\u5965\u3002<\/p>\n<p><br \/><\/p>\n<p>\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1 – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/p>\n<p><br \/><\/p>\n<p>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a18502195490\uff08\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/p>\n<\/section>\n<section style=\"text-align: right;justify-content: flex-end;display: flex;flex-flow: row;transform: translate3d(40px, 0px, 0px);margin-bottom: -40px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"transform: rotateZ(45deg);\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<section style=\"display: inline-block;width: 26px;height: 26px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);border-left: 1px solid rgb(160, 160, 160);border-bottom-left-radius: 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&mid=2650761303&idx=2&sn=6f73471d1e82c56c0b2160d0fcd32d53&scene=21#wechat_redirect\" textvalue=\"\" linktype=\"image\" imgurl=\"https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/0?wx_fmt=jpeg\" imgdata=\"[object Object]\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_pBN4r4MaBCk9p9v16yZwvMvS5nM7sQ-9.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSYCoxhZfqr5L0fxy3C9eSFdt72DV7zqsmrNaiaaoOhic3sHzUpBN4r4MaBCk9p9v16yZwvMvS5nM7sQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"895\" style=\"\" data-recalc-dims=\"1\" \/><\/span><\/a><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3488888888888889\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_wGGHoIUWPWSIgBgzuWA7zggZhslM2g-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/KDvR2NrGicSbGdkTKlXI3tbocicP8GUCzibJV5ibjQs6D7lQhs1pnDYZQiauBwGGHoIUWPWSIgBgzuWA7zggZhslM2g\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p powered-by=\"xiumi.us\">\uff08\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8df3\u8f6c\uff09<\/p>\n<section style=\"text-align: left;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;min-width: 10%;vertical-align: top;padding-right: 20px;\">\n<section style=\"transform: translate3d(20px, 0px, 0px);margin: 10px 0% -30px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(62, 62, 62);text-align: justify;font-size: 12px;\">\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.866\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_hTb9TCBmE3ZicYpP2jqDjWX6xDRuTQ.gif?resize=18%2C15\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_gif\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSj0ZoyOLjZBYjCtRku4tPJdKviahTb9TCBmE3ZicYpP2jqDjWX6xDRuTQ\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"500\" height=\"15\" style=\"width: 18px;height: 15px;\" width=\"18\" data-recalc-dims=\"1\" \/> \u70b9\u4e00\u4e0b\u9605\u8bfb\u539f\u6587\u4e86\u89e3\u66f4\u591a\u8d44\u8baf<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 50px;height: 40px;vertical-align: top;overflow: hidden;\">\n<section style=\"text-align: center;margin-right: 0%;margin-left: 0%;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.7709090909090909\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_QM3WCXd8qvGSWQt39mNFgcFB8x6CfA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/KDvR2NrGicSb7ARKFDGUia5uQoMlCrYOzSL2jMD7ichFZu3oAMIdicF0lCfyQM3WCXd8qvGSWQt39mNFgcFB8x6CfA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"275\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u751f\u4fe1\u6587\u7ae0\u5fc5\u5907\u5206\u6790\u5185\u5bb9\uff0c\u5bf9\u4e9a\u578b\u5229\u7528MCPcounter\u548cCIBERSORT\u4e24\u79cd\u7b97\u6cd5\u8fdb\u884c\u514d\u75ab\u6d78\u6da6\u5206\u6790 \u5c0f\u82b1&nbs […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57820"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=57820"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57820\/revisions"}],"predecessor-version":[{"id":57832,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57820\/revisions\/57832"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=57820"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=57820"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=57820"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}