<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 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#33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation 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0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar 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rgb(255, 255, 255);\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 403.391px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;\">\n<section style=\"margin-top: 10px;\">\n<section style=\"text-align: center;\">\n<section style=\"font-size: 16px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;vertical-align: top;width: 70.0348%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 70.0348% !important;\">\n<section style=\"margin-top: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 29.9652%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 29.9652% !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;line-height: 0;\">\n<section>\n<section style=\"display: inline-block;vertical-align: middle;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 0;\">\n<section style=\"width: 111px;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 1.75em;text-indent: 0em;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;height: auto;width: 111px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_GBhsibRAgNiaQsH4ichdP9ibEhHMFA-100.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYngnlT6jiavu08Bvp2iblzGf3IkoBD9NGBhsibRAgNiaQsH4ichdP9ibEhHMFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"400\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-role=\"list\">\n<section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 8px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"padding-left: 8px;display: inline-block;width: 578px;vertical-align: top;border-left: 3px solid rgb(219, 219, 219);border-bottom-left-radius: 0px;align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"color: rgba(0, 0, 0, 0.5);text-align: justify;\">\n<p><span style=\"letter-spacing: 0.578px;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\">hello\uff01\u5c0f\u679c\u53c8\u6765\u5566\uff01<\/span><\/span><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\">\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u524d\u60c5\u56de\u987e\uff1a\u4e0a\u671f\u6211\u4eec\u5229\u7528gatk\u751f\u6210\u4e86\u7d22\u5f15\u6587\u4ef6\u5e76\u5bf9sam\u6587\u4ef6\u8fdb\u884c\u4e86\u6392\u5e8f\uff0c\u6298\u8d77\u6211\u4eec\u63a5\u7740\u5f80\u4e0b\u8d70\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e00\u4e2a\u5c0ftip\uff1a\u7d22\u5f15\u6587\u4ef6\u6700\u597d\u8981\u548c\u53c2\u8003\u57fa\u56e0\u7ec4\u7684fasta\u6587\u653e\u5230\u540c\u4e00\u4e2a\u76ee\u5f55\u54e6\uff01\u4e0d\u7136\u540e\u9762\u4f1a\u51fa\u9519\u7684\uff0c\u5c0f\u679c\u7684\u8840\u6cea\u6559\u8bad\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u597d\u4e86\u6211\u4eec\u7ee7\u7eed\u5427\uff0c\u73b0\u5728\u6211\u4eec\u9700\u8981\u5229\u7528GATK\u7684MarkDuplicates\u5de5\u5177\u53bb\u9664PCR\u91cd\u590d\uff0c\u5e76\u5c06\u7ed3\u679c\u5b58\u50a8\u5230\u6307\u5b9a\u7684\u8f93\u51fa\u6587\u4ef6\u4e2d\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span> sample in `cat \/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/sample.txt`; <span class=\"code-snippet__keyword\">do<\/span> #\u5bf9sample.txt\u4e2d\u7684\u6bcf\u4e00\u4e2a\u6837\u672c\u5faa\u73af\u64cd\u4f5c<\/span><\/code><code><span class=\"code-snippet_outer\"> echo ${sample} #\u8f93\u51fa\u5f53\u524d\u6837\u672c\u7684\u540d\u79f0<\/span><\/code><code><span class=\"code-snippet_outer\"> gatk --java-options <span class=\"code-snippet__string\">\"-Xmx16g -Djava.io.tmpdir=.\/tmp\"<\/span> MarkDuplicates #\u8c03\u7528GATK\u7684MarkDuplicates\u5de5\u5177<\/span><\/code><code><span class=\"code-snippet_outer\"> -I \/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/tair_sam\/${sample}.sort.bam #\u8f93\u5165\u6587\u4ef6\u4e3a\u6392\u5e8f\u540e\u7684BAM\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\"> -M \/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/tair_sam\/sort\/${sample}.metrics --CREATE_INDEX #\u6307\u5b9a\u8f93\u51fa\u91cd\u590d\u4fe1\u606f\u7684\u6587\u4ef6\u4ee5\u53ca\u8f93\u51fa\u6587\u4ef6\u7684\u521b\u5efa<\/span><\/code><code><span class=\"code-snippet_outer\"> -O \/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/tair_sam\/sort\/${sample}.sort.MarkDup.bam #\u8f93\u51fa\u6587\u4ef6\u540d<\/span><\/code><code><span class=\"code-snippet_outer\">done<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5176\u4e2d\uff0c${sample}\u4ee3\u8868sample.txt\u6587\u4ef6\u4e2d\u6bcf\u4e00\u884c\u6240\u5b58\u50a8\u7684\u6837\u672c\u540d\u79f0\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">-I\u9009\u9879\u7528\u4e8e\u6307\u5b9a\u8f93\u5165\u7684BAM\u6587\u4ef6\uff0c-M\u9009\u9879\u6307\u5b9a\u8f93\u51fa\u91cd\u590d\u4fe1\u606f\u7684\u6587\u4ef6\uff0c–CREATE_INDEX\u9009\u9879\u6307\u5b9a\u8f93\u51fa\u6587\u4ef6\u7684\u540c\u65f6\u4e5f\u521b\u5efa\u5176\u5bf9\u5e94\u7684\u7d22\u5f15\u6587\u4ef6\uff0c-O\u9009\u9879\u6307\u5b9a\u8f93\u51fa\u6587\u4ef6\u540d\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">–java-options “-Xmx16g -Djava.io.tmpdir=.\/tmp”\u9009\u9879\u5219\u662f\u5728\u8fd0\u884cGATK\u65f6\u4e3aJava\u865a\u62df\u673a\u6307\u5b9a\u53c2\u6570\uff0c\u5176\u4e2d-Xmx16g\u6307\u5b9a\u4e86\u6700\u5927\u5806\u5185\u5b58\u4e3a16GB\uff0c-Djava.io.tmpdir=.\/tmp\u6307\u5b9a\u4e86\u4e34\u65f6\u6587\u4ef6\u7684\u76ee\u5f55\u4e3a\u5f53\u524d\u5de5\u4f5c\u76ee\u5f55\u4e0b\u7684tmp\u6587\u4ef6\u5939\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\u6211\u4eec\u5bf9\u6bcf\u4e2a\u6837\u672c\u8fdb\u884cHaplotypeCaller\u53d8\u5f02\u68c0\u6d4b\uff0c\u5e76\u751f\u6210GVCF\u683c\u5f0f\u7684\u6587\u4ef6\uff0c\u6ce8\u610f\u66ff\u6362\u81ea\u5df1\u7684\u6587\u4ef6\u8def\u5f84\uff1a<\/span><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">REF=\/media\/desk16\/iyun003\/download\/ninanjie_data\/GCF_000001735<span class=\"code-snippet__number\">.4<\/span>_TAIR10<span class=\"code-snippet__number\">.1<\/span>_genomic.fna<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span> sample in `cat \/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/sample.txt`;<span class=\"code-snippet__keyword\">do<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> echo <span class=\"code-snippet__string\">\"$sample\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> gatk --java-options <span class=\"code-snippet__string\">\"-Xmx4g\"<\/span> HaplotypeCaller <\/span><\/code><code><span class=\"code-snippet_outer\"> -R <span class=\"code-snippet__string\">\"${REF}\"<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> -I <span class=\"code-snippet__string\">\"\/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/tair_sam\/sort\/${sample}.sort.MarkDup.bam\"<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> -O <span class=\"code-snippet__string\">\"\/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/gvcf\/${sample}.g.vcf.gz\"<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> -ERC GVCF<\/span><\/code><code><span class=\"code-snippet_outer\">done<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5176\u4e2d\uff1aREF \u6307\u5b9a\u53c2\u8003\u57fa\u56e0\u7ec4\u6587\u4ef6\u8def\u5f84<\/span><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">sample \u6307\u5b9a\u6837\u672c\u540d\u79f0\uff0c\u901a\u8fc7\u8bfb\u53d6 sample.txt \u6587\u4ef6\u83b7\u53d6<\/span><\/code><code><span class=\"code-snippet_outer\">gatk --java-options <span class=\"code-snippet__string\">\"-Xmx4g\"<\/span> HaplotypeCaller \u8fd0\u884c GATK \u7684 HaplotypeCaller \u53d8\u5f02\u68c0\u6d4b\u7a0b\u5e8f\uff0c--java-options <span class=\"code-snippet__string\">\"-Xmx4g\"<\/span> \u6307\u5b9a\u7a0b\u5e8f\u8fd0\u884c\u65f6\u6700\u5927\u5360\u7528<span class=\"code-snippet__number\">4<\/span>GB\u5185\u5b58<\/span><\/code><code><span class=\"code-snippet_outer\">-R <span class=\"code-snippet__string\">\"${REF}\"<\/span> \u6307\u5b9a\u53c2\u8003\u57fa\u56e0\u7ec4\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">-I <span class=\"code-snippet__string\">\"\/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/tair_sam\/sort\/${sample}.sort.MarkDup.bam\"<\/span> \u6307\u5b9a\u8f93\u5165BAM\u6587\u4ef6\u8def\u5f84<\/span><\/code><code><span class=\"code-snippet_outer\">-O <span class=\"code-snippet__string\">\"\/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/gvcf\/${sample}.g.vcf.gz\"<\/span> \u6307\u5b9a\u8f93\u51fa\u6587\u4ef6\u8def\u5f84\u53ca\u6587\u4ef6\u540d<\/span><\/code><code><span class=\"code-snippet_outer\">-ERC GVCF \u6307\u5b9a\u8f93\u51fa\u4e3aGVCF\u683c\u5f0f<\/span><\/code><code><span class=\"code-snippet_outer\">\u8fd9\u4e00\u6b65\u8fd0\u884c\u65f6\u95f4\u8f83\u4e45\uff0c\u53ef\u4ee5\u901a\u8fc7\u67e5\u770b\u8fd0\u884c\u65e5\u5fd7\u6765\u4e86\u89e3\u8fd0\u884c\u60c5\u51b5\u54e6\uff0c\u5c0f\u679c\u628a\u5b83\u653e\u5230\u811a\u672c\u91cc\u540e\u53f0\u8fd0\u884c\uff0c\u5c31\u4e0d\u7528\u62c5\u5fc3\u610f\u5916\u4e2d\u65ad\u4e86\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">nohup bash gvcf.sh > gvcf.<span class=\"code-snippet__built_in\">log<\/span> &<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u67e5\u770b\u65e5\u5fd7<em><strong>cat gvcf.log<\/strong><\/em><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5c0f\u679c\u8fd9\u8fb9\u653e\u4e00\u4e0b\u8fd0\u884c\u8fc7\u7a0b\u4e2d\u7684\u7ed3\u679c\uff0c\u53ef\u4ee5\u770b\u5230\u5176\u4ed6\u7ed3\u679c\u8fd8\u6ca1\u6709\u51fa\u6765\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u597d\u5566\uff0c\u4eca\u5929\u7684\u5185\u5bb9\u6682\u65f6\u5c31\u5230\u8fd9\u91cc\u4e86\uff0c\u6211\u4eec\u4e0b\u671f\u7ee7\u7eed\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: left;white-space: normal;\"><span style=\"font-size: 14px;\"><strong><em>\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1 – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/em><\/strong><\/span><\/p>\n<p style=\"text-align: left;white-space: normal;\"><span style=\"font-size: 14px;\"><strong><em>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a18502195490\uff08\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/em><\/strong><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<section data-tools=\"135\u7f16\u8f91\u5668\" data-id=\"105054\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;font-size: 16px;\">\n<section style=\"margin: 10px auto;text-align: center;\">\n<section style=\"padding-top: 30px;padding-bottom: 30px;background-color: rgb(242, 246, 255);border-radius: 7px;\">\n<section style=\"display: flex;justify-content: space-evenly;align-items: center;\">\n<section data-width=\"26%\" style=\"width: 150.266px;\">\n<section style=\"padding: 7px;border-radius: 15px;box-shadow: rgba(169, 187, 223, 0.45) 4px 4px 8px, rgb(255, 255, 255) -2.5px -2.5px 5px;\">\n<section data-width=\"100%\" style=\"width: 136.266px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"136\" data-cropsely1=\"0\" data-cropsely2=\"137\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_jEYQoRbKh3HUg3IGgzymibpTnmaM6Q-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwIX3hF1ZZeqvXLknM5Oq7CDG9YFpmvU1ge12vc3NvibhViceQjEYQoRbKh3HUg3IGgzymibpTnmaM6Q\/640?wx_fmt=jpeg'\" data-type=\"png\" data-w=\"430\" data-width=\"100%\" style=\"height: 137px;vertical-align: inherit;width: 137px;display: block;border-radius: 7px;\" title=\"\u5c0f\u4e91-\u5fae\u4fe1\u4e8c\u7ef4\u7801.png\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section data-width=\"62%\" style=\"width: 358.359px;\">\n<section style=\"display: inline-block;\">\n<section style=\"padding: 7px 15px;border-radius: 20px;box-shadow: rgba(169, 187, 223, 0.45) 6px 6px 8px inset, rgb(255, 255, 255) -2.5px -2.5px 5px inset;\">\n<section data-brushtype=\"text\" style=\"font-size: 14px;letter-spacing: 1.5px;color: rgb(111, 133, 172);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><span style=\"letter-spacing: 1.5px;\">\u626b\u7801\u52a0\u5c0f\u679c<\/span><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><span style=\"letter-spacing: 1.5px;\">\u9886\u53d6\u751f\u4fe1\u5927\u793c\u5305<\/span><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 <\/span><span style=\"letter-spacing: 0.5px;text-indent: 0em;\"><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5229\u7528gatk\u8fdb\u884c\u53d8\u5f02\u68c0\u6d4b\u7684n\u6b65\u8d70\u6218\u7565\u4e4b\u4e8c \u539f\u521b \u5c0f\u679c \u751f\u4fe1\u679c&nb […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57964"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=57964"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57964\/revisions"}],"predecessor-version":[{"id":57967,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57964\/revisions\/57967"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=57964"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=57964"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=57964"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

{"id":57964,"date":"2024-06-16T20:55:59","date_gmt":"2024-06-16T12:55:59","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=57964"},"modified":"2024-06-16T20:55:59","modified_gmt":"2024-06-16T12:55:59","slug":"%e5%88%a9%e7%94%a8gatk%e8%bf%9b%e8%a1%8c%e5%8f%98%e5%bc%82%e6%a3%80%e6%b5%8b%e7%9a%84n%e6%ad%a5%e8%b5%b0%e6%88%98%e7%95%a5%e4%b9%8b%e4%ba%8c","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=57964","title":{"rendered":"\u5229\u7528gatk\u8fdb\u884c\u53d8\u5f02\u68c0\u6d4b\u7684n\u6b65\u8d70\u6218\u7565\u4e4b\u4e8c"},"content":{"rendered":"

\n
\n<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner,body{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media{padding:20px;overflow:hidden;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}@media screen and (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media_content{overflow:hidden;color:#333;font-size:17px;word-wrap:break-word;-webkit-hyphens:auto;-ms-hyphens:auto;hyphens:auto;text-align:justify;position:relative;z-index:0}.rich_media_content *{max-width:100%!important;box-sizing:border-box!important;-webkit-box-sizing:border-box!important;word-wrap:break-word!important}.rich_media_content p{clear:both;min-height:1em}.rich_media_content em{font-style:italic}.rich_media_content fieldset{min-width:0}.rich_media_content .list-paddingleft-1,.rich_media_content .list-paddingleft-2,.rich_media_content .list-paddingleft-3{padding-left:2.2em}.rich_media_content .list-paddingleft-1 .list-paddingleft-2,.rich_media_content .list-paddingleft-2 .list-paddingleft-2,.rich_media_content .list-paddingleft-3 .list-paddingleft-2{padding-left:30px}.rich_media_content .list-paddingleft-1{padding-left:1.2em}.rich_media_content .list-paddingleft-3{padding-left:3.2em}.rich_media_content .code-snippet,.rich_media_content .code-snippet__fix{max-width:1000%!important}.rich_media_content .code-snippet *,.rich_media_content .code-snippet__fix *{max-width:1000%!important}.rich_media_title{font-size:22px;line-height:1.4;margin-bottom:13px;padding-bottom:13px;border-bottom:1px solid #e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag .code-snippet__attr{color:#0e9ce5}.code-snippet__string,.code-snippet__doctag{color:#d14}.code-snippet__title,.code-snippet__section,.code-snippet__selector-id{color:#d14}.code-snippet__subst{font-weight:normal}.code-snippet__type,.code-snippet__class .code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u5229\u7528gatk\u8fdb\u884c\u53d8\u5f02\u68c0\u6d4b\u7684n\u6b65\u8d70\u6218\u7565\u4e4b\u4e8c\",\"time\":\"2023-06-13 19:00:48\",\"timeStamp\":\"1686654048\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247488317&idx=1&sn=e45b442b47faa0bcef7a3ed9cc876667&chksm=cf7c83f8f80b0aee8df32b4c5153a7594cceb806ee2958dffc175f057f9a542f1cd330b74d5e#rd\" target=\"_blank\" rel=\"noopener\">\u5229\u7528gatk\u8fdb\u884c\u53d8\u5f02\u68c0\u6d4b\u7684n\u6b65\u8d70\u6218\u7565\u4e4b\u4e8c<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-06-13 19:00:48<\/em><\/div>\n<p><content><\/p>\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;font-size: 16px;\">\n<section>\n<section>\n<section>\n<section>\n<section>\n<section data-width=\"100%\" style=\"width: 578px;flex: 0 0 100%;opacity: 1;\">\n<section style=\"line-height: 1.8;\">\n<section>\n<section>\n<section style=\"margin-top: 10px;margin-bottom: 20px;display: flex;flex-flow: row nowrap;text-align: center;justify-content: center;\">\n<section style=\"display: inline-block;width: 578px;vertical-align: top;border-style: solid;border-width: 1px;border-color: rgb(67, 67, 67);flex: 0 0 auto;align-self: flex-start;height: auto;box-shadow: rgb(255, 219, 118) 6px 6px 0px;background-color: rgb(255, 255, 255);\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 403.391px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;\">\n<section style=\"margin-top: 10px;\">\n<section style=\"text-align: center;\">\n<section style=\"font-size: 16px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;vertical-align: top;width: 70.0348%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 70.0348% !important;\">\n<section style=\"margin-top: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 29.9652%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 29.9652% !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;line-height: 0;\">\n<section>\n<section style=\"display: inline-block;vertical-align: middle;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 0;\">\n<section style=\"width: 111px;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 1.75em;text-indent: 0em;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;height: auto;width: 111px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_GBhsibRAgNiaQsH4ichdP9ibEhHMFA-100.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYngnlT6jiavu08Bvp2iblzGf3IkoBD9NGBhsibRAgNiaQsH4ichdP9ibEhHMFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"400\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-role=\"list\">\n<section>\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 8px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"padding-left: 8px;display: inline-block;width: 578px;vertical-align: top;border-left: 3px solid rgb(219, 219, 219);border-bottom-left-radius: 0px;align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"color: rgba(0, 0, 0, 0.5);text-align: justify;\">\n<p><span style=\"letter-spacing: 0.578px;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;\">hello\uff01\u5c0f\u679c\u53c8\u6765\u5566\uff01<\/span><\/span><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\">\n<p style=\"text-align: center;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u524d\u60c5\u56de\u987e\uff1a\u4e0a\u671f\u6211\u4eec\u5229\u7528gatk\u751f\u6210\u4e86\u7d22\u5f15\u6587\u4ef6\u5e76\u5bf9sam\u6587\u4ef6\u8fdb\u884c\u4e86\u6392\u5e8f\uff0c\u6298\u8d77\u6211\u4eec\u63a5\u7740\u5f80\u4e0b\u8d70\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e00\u4e2a\u5c0ftip\uff1a\u7d22\u5f15\u6587\u4ef6\u6700\u597d\u8981\u548c\u53c2\u8003\u57fa\u56e0\u7ec4\u7684fasta\u6587\u653e\u5230\u540c\u4e00\u4e2a\u76ee\u5f55\u54e6\uff01\u4e0d\u7136\u540e\u9762\u4f1a\u51fa\u9519\u7684\uff0c\u5c0f\u679c\u7684\u8840\u6cea\u6559\u8bad\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u597d\u4e86\u6211\u4eec\u7ee7\u7eed\u5427\uff0c\u73b0\u5728\u6211\u4eec\u9700\u8981\u5229\u7528GATK\u7684MarkDuplicates\u5de5\u5177\u53bb\u9664PCR\u91cd\u590d\uff0c\u5e76\u5c06\u7ed3\u679c\u5b58\u50a8\u5230\u6307\u5b9a\u7684\u8f93\u51fa\u6587\u4ef6\u4e2d\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span> sample in `cat \/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/sample.txt`; <span class=\"code-snippet__keyword\">do<\/span> #\u5bf9sample.txt\u4e2d\u7684\u6bcf\u4e00\u4e2a\u6837\u672c\u5faa\u73af\u64cd\u4f5c<\/span><\/code><code><span class=\"code-snippet_outer\"> echo ${sample} #\u8f93\u51fa\u5f53\u524d\u6837\u672c\u7684\u540d\u79f0<\/span><\/code><code><span class=\"code-snippet_outer\"> gatk --java-options <span class=\"code-snippet__string\">\"-Xmx16g -Djava.io.tmpdir=.\/tmp\"<\/span> MarkDuplicates #\u8c03\u7528GATK\u7684MarkDuplicates\u5de5\u5177<\/span><\/code><code><span class=\"code-snippet_outer\"> -I \/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/tair_sam\/${sample}.sort.bam #\u8f93\u5165\u6587\u4ef6\u4e3a\u6392\u5e8f\u540e\u7684BAM\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\"> -M \/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/tair_sam\/sort\/${sample}.metrics --CREATE_INDEX #\u6307\u5b9a\u8f93\u51fa\u91cd\u590d\u4fe1\u606f\u7684\u6587\u4ef6\u4ee5\u53ca\u8f93\u51fa\u6587\u4ef6\u7684\u521b\u5efa<\/span><\/code><code><span class=\"code-snippet_outer\"> -O \/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/tair_sam\/sort\/${sample}.sort.MarkDup.bam #\u8f93\u51fa\u6587\u4ef6\u540d<\/span><\/code><code><span class=\"code-snippet_outer\">done<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5176\u4e2d\uff0c${sample}\u4ee3\u8868sample.txt\u6587\u4ef6\u4e2d\u6bcf\u4e00\u884c\u6240\u5b58\u50a8\u7684\u6837\u672c\u540d\u79f0\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">-I\u9009\u9879\u7528\u4e8e\u6307\u5b9a\u8f93\u5165\u7684BAM\u6587\u4ef6\uff0c-M\u9009\u9879\u6307\u5b9a\u8f93\u51fa\u91cd\u590d\u4fe1\u606f\u7684\u6587\u4ef6\uff0c–CREATE_INDEX\u9009\u9879\u6307\u5b9a\u8f93\u51fa\u6587\u4ef6\u7684\u540c\u65f6\u4e5f\u521b\u5efa\u5176\u5bf9\u5e94\u7684\u7d22\u5f15\u6587\u4ef6\uff0c-O\u9009\u9879\u6307\u5b9a\u8f93\u51fa\u6587\u4ef6\u540d\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">–java-options “-Xmx16g -Djava.io.tmpdir=.\/tmp”\u9009\u9879\u5219\u662f\u5728\u8fd0\u884cGATK\u65f6\u4e3aJava\u865a\u62df\u673a\u6307\u5b9a\u53c2\u6570\uff0c\u5176\u4e2d-Xmx16g\u6307\u5b9a\u4e86\u6700\u5927\u5806\u5185\u5b58\u4e3a16GB\uff0c-Djava.io.tmpdir=.\/tmp\u6307\u5b9a\u4e86\u4e34\u65f6\u6587\u4ef6\u7684\u76ee\u5f55\u4e3a\u5f53\u524d\u5de5\u4f5c\u76ee\u5f55\u4e0b\u7684tmp\u6587\u4ef6\u5939\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\u6211\u4eec\u5bf9\u6bcf\u4e2a\u6837\u672c\u8fdb\u884cHaplotypeCaller\u53d8\u5f02\u68c0\u6d4b\uff0c\u5e76\u751f\u6210GVCF\u683c\u5f0f\u7684\u6587\u4ef6\uff0c\u6ce8\u610f\u66ff\u6362\u81ea\u5df1\u7684\u6587\u4ef6\u8def\u5f84\uff1a<\/span><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">REF=\/media\/desk16\/iyun003\/download\/ninanjie_data\/GCF_000001735<span class=\"code-snippet__number\">.4<\/span>_TAIR10<span class=\"code-snippet__number\">.1<\/span>_genomic.fna<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span> sample in `cat \/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/sample.txt`;<span class=\"code-snippet__keyword\">do<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> echo <span class=\"code-snippet__string\">\"$sample\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> gatk --java-options <span class=\"code-snippet__string\">\"-Xmx4g\"<\/span> HaplotypeCaller <\/span><\/code><code><span class=\"code-snippet_outer\"> -R <span class=\"code-snippet__string\">\"${REF}\"<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> -I <span class=\"code-snippet__string\">\"\/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/tair_sam\/sort\/${sample}.sort.MarkDup.bam\"<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> -O <span class=\"code-snippet__string\">\"\/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/gvcf\/${sample}.g.vcf.gz\"<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> -ERC GVCF<\/span><\/code><code><span class=\"code-snippet_outer\">done<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5176\u4e2d\uff1aREF \u6307\u5b9a\u53c2\u8003\u57fa\u56e0\u7ec4\u6587\u4ef6\u8def\u5f84<\/span><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">sample \u6307\u5b9a\u6837\u672c\u540d\u79f0\uff0c\u901a\u8fc7\u8bfb\u53d6 sample.txt \u6587\u4ef6\u83b7\u53d6<\/span><\/code><code><span class=\"code-snippet_outer\">gatk --java-options <span class=\"code-snippet__string\">\"-Xmx4g\"<\/span> HaplotypeCaller \u8fd0\u884c GATK \u7684 HaplotypeCaller \u53d8\u5f02\u68c0\u6d4b\u7a0b\u5e8f\uff0c--java-options <span class=\"code-snippet__string\">\"-Xmx4g\"<\/span> \u6307\u5b9a\u7a0b\u5e8f\u8fd0\u884c\u65f6\u6700\u5927\u5360\u7528<span class=\"code-snippet__number\">4<\/span>GB\u5185\u5b58<\/span><\/code><code><span class=\"code-snippet_outer\">-R <span class=\"code-snippet__string\">\"${REF}\"<\/span> \u6307\u5b9a\u53c2\u8003\u57fa\u56e0\u7ec4\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">-I <span class=\"code-snippet__string\">\"\/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/tair_sam\/sort\/${sample}.sort.MarkDup.bam\"<\/span> \u6307\u5b9a\u8f93\u5165BAM\u6587\u4ef6\u8def\u5f84<\/span><\/code><code><span class=\"code-snippet_outer\">-O <span class=\"code-snippet__string\">\"\/media\/desk16\/iyun003\/GBS_test\/ERR_SRA\/gvcf\/${sample}.g.vcf.gz\"<\/span> \u6307\u5b9a\u8f93\u51fa\u6587\u4ef6\u8def\u5f84\u53ca\u6587\u4ef6\u540d<\/span><\/code><code><span class=\"code-snippet_outer\">-ERC GVCF \u6307\u5b9a\u8f93\u51fa\u4e3aGVCF\u683c\u5f0f<\/span><\/code><code><span class=\"code-snippet_outer\">\u8fd9\u4e00\u6b65\u8fd0\u884c\u65f6\u95f4\u8f83\u4e45\uff0c\u53ef\u4ee5\u901a\u8fc7\u67e5\u770b\u8fd0\u884c\u65e5\u5fd7\u6765\u4e86\u89e3\u8fd0\u884c\u60c5\u51b5\u54e6\uff0c\u5c0f\u679c\u628a\u5b83\u653e\u5230\u811a\u672c\u91cc\u540e\u53f0\u8fd0\u884c\uff0c\u5c31\u4e0d\u7528\u62c5\u5fc3\u610f\u5916\u4e2d\u65ad\u4e86\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">nohup bash gvcf.sh > gvcf.<span class=\"code-snippet__built_in\">log<\/span> &<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u67e5\u770b\u65e5\u5fd7<em><strong>cat gvcf.log<\/strong><\/em><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5c0f\u679c\u8fd9\u8fb9\u653e\u4e00\u4e0b\u8fd0\u884c\u8fc7\u7a0b\u4e2d\u7684\u7ed3\u679c\uff0c\u53ef\u4ee5\u770b\u5230\u5176\u4ed6\u7ed3\u679c\u8fd8\u6ca1\u6709\u51fa\u6765\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u597d\u5566\uff0c\u4eca\u5929\u7684\u5185\u5bb9\u6682\u65f6\u5c31\u5230\u8fd9\u91cc\u4e86\uff0c\u6211\u4eec\u4e0b\u671f\u7ee7\u7eed\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: left;white-space: normal;\"><span style=\"font-size: 14px;\"><strong><em>\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1 – \u5b66\u751f\u7269\u4fe1\u606f\u5b66 (biocloudservice.com)<\/em><\/strong><\/span><\/p>\n<p style=\"text-align: left;white-space: normal;\"><span style=\"font-size: 14px;\"><strong><em>\u5982\u679c\u60f3\u7528\u670d\u52a1\u5668\u53ef\u4ee5\u8054\u7cfb\u5fae\u4fe1\uff1a18502195490\uff08\u5feb\u6765\u8054\u7cfb\u6211\u4eec\u4f7f\u7528\u5427\uff01\uff09<\/em><\/strong><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<section data-tools=\"135\u7f16\u8f91\u5668\" data-id=\"105054\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;font-size: 16px;\">\n<section style=\"margin: 10px auto;text-align: center;\">\n<section style=\"padding-top: 30px;padding-bottom: 30px;background-color: rgb(242, 246, 255);border-radius: 7px;\">\n<section style=\"display: flex;justify-content: space-evenly;align-items: center;\">\n<section data-width=\"26%\" style=\"width: 150.266px;\">\n<section style=\"padding: 7px;border-radius: 15px;box-shadow: rgba(169, 187, 223, 0.45) 4px 4px 8px, rgb(255, 255, 255) -2.5px -2.5px 5px;\">\n<section data-width=\"100%\" style=\"width: 136.266px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"136\" data-cropsely1=\"0\" data-cropsely2=\"137\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_jEYQoRbKh3HUg3IGgzymibpTnmaM6Q-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwIX3hF1ZZeqvXLknM5Oq7CDG9YFpmvU1ge12vc3NvibhViceQjEYQoRbKh3HUg3IGgzymibpTnmaM6Q\/640?wx_fmt=jpeg'\" data-type=\"png\" data-w=\"430\" data-width=\"100%\" style=\"height: 137px;vertical-align: inherit;width: 137px;display: block;border-radius: 7px;\" title=\"\u5c0f\u4e91-\u5fae\u4fe1\u4e8c\u7ef4\u7801.png\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section data-width=\"62%\" style=\"width: 358.359px;\">\n<section style=\"display: inline-block;\">\n<section style=\"padding: 7px 15px;border-radius: 20px;box-shadow: rgba(169, 187, 223, 0.45) 6px 6px 8px inset, rgb(255, 255, 255) -2.5px -2.5px 5px inset;\">\n<section data-brushtype=\"text\" style=\"font-size: 14px;letter-spacing: 1.5px;color: rgb(111, 133, 172);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><span style=\"letter-spacing: 1.5px;\">\u626b\u7801\u52a0\u5c0f\u679c<\/span><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><span style=\"letter-spacing: 1.5px;\">\u9886\u53d6\u751f\u4fe1\u5927\u793c\u5305<\/span><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 <\/span><span style=\"letter-spacing: 0.5px;text-indent: 0em;\"><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5229\u7528gatk\u8fdb\u884c\u53d8\u5f02\u68c0\u6d4b\u7684n\u6b65\u8d70\u6218\u7565\u4e4b\u4e8c \u539f\u521b \u5c0f\u679c \u751f\u4fe1\u679c&nb […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57964"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=57964"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57964\/revisions"}],"predecessor-version":[{"id":57967,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/57964\/revisions\/57967"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=57964"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=57964"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=57964"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}