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1px;\">\u7f51\u5740\u53bb\u8bbf\u95eeScType\u4ea4\u4e92\u5f0f Web \u5e73\u53f0\u3002ScType\u5e73\u53f0\u4e3a\u5355\u7ec6\u80de RNA-seq \u6570\u636e\u5206\u6790\u63d0\u4f9b\u4e86\u5b8c\u6574\u7684\u7ba1\u9053\uff0c\u5305\u62ec\u6570\u636e\u5904\u7406\u3001\u5f52\u4e00\u5316\u3001\u805a\u7c7b\u548c\u7ec6\u80de\u7c7b\u578b\u6ce8\u91ca\u3002\u5177\u4f53\u6b65\u9aa4\u5982\u4e0b<\/span><\/section>\n<section style=\"text-align: justify;font-size: 10.5pt;font-family: \u7b49\u7ebf;font-weight: normal;margin-left: 36pt;text-indent: -36pt;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-size: 16px;font-variant: normal;text-transform: none;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\uff081\uff09\u5355\u51fb\u4e0a\u4f20\u9009\u9879\u5361\u6765\u4e0a\u4f20\u6570\u636e\u96c6\u3002\u6709\u5173\u8be6\u7ec6\u4fe1\u606f\uff0c\u8bf7\u53c2\u9605\u6280\u672f\u6587\u6863\uff08<\/span><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">http:\/\/sctype.app<\/span><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\uff09\u3002<\/span><\/section>\n<section style=\"text-align: justify;font-size: 10.5pt;font-family: \u7b49\u7ebf;font-weight: normal;margin-left: 36pt;text-indent: -36pt;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-size: 16px;font-variant: normal;text-transform: none;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\uff082\uff09\u9009\u62e9\u60a8\u7684scRNA-seq\u6570\u636e\u6587\u4ef6\u6216\u5c06\u5176\u62d6\u5230\u84dd\u8272\u533a\u57df\u3002ScType\u4e3ascRNA-seq\u6570\u636e\u63d0\u4f9b\u4e86\u591a\u79cd\u8f93\u5165\u6570\u636e\u683c\u5f0f\u3002<\/span><\/section>\n<section style=\"text-align: justify;font-size: 10.5pt;font-family: \u7b49\u7ebf;font-weight: normal;margin-left: 36pt;text-indent: -36pt;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-size: 16px;font-variant: normal;text-transform: none;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\uff083\uff09\u6307\u5b9a\u5206\u6790\u9009\u9879\u3002<\/span><\/section>\n<section style=\"text-align: justify;font-size: 10.5pt;font-family: \u7b49\u7ebf;font-weight: normal;margin-left: 36pt;text-indent: -36pt;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-size: 16px;font-variant: normal;text-transform: none;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\uff084\uff09\u4e0a\u4f20\u6570\u636e\u548c\u6307\u5b9a\u7684\u5206\u6790\u9009\u9879\u540e\uff0c\u5355\u51fb\u5f00\u59cb\u5206\u6790\u6309\u94ae\u3002<\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100016483\" data-ratio=\"0.7685492801771872\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_gOk1It7yibicVgUfSyhXD7ZzdbawgA.png?resize=553%2C425\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzwiazfwqaFEHgqLAZibbKOhBJ2bKRXumBphAvY0HBiaWUM4HxbkgOk1It7yibicVgUfSyhXD7ZzdbawgA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"903\" height=\"425 .6099853515625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><span style=\"font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;\">         <o:p> <\/o:p><\/span>    <\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><o:page><\/o:page><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-weight: bold;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">ScType\u4ee3\u7801\u5b9e\u73b0\u6d41\u7a0b<\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(47, 85, 151);font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u5de5\u4f5c\u73af\u5883\uff0c\u8fd9\u91cc\u8981\u6539\u6210\u81ea\u5df1\u7684\u5de5\u4f5c\u73af\u5883\u54e6\uff01\uff01\uff01\uff01<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">setwd<\/span>(<span class=\"code-snippet__string\">\"D:\\work\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u52a0\u8f7dR\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">lapply<\/span>(c(<span class=\"code-snippet__string\">\"dplyr\"<\/span>,<span class=\"code-snippet__string\">\"Seurat\"<\/span>,<span class=\"code-snippet__string\">\"HGNChelper\"<\/span>), library, character.only = T)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6570\u636e\u4e0b\u8f7d\uff1ahttps:\/\/cf.10xgenomics.com\/samples\/cell\/pbmc3k\/pbmc3k_filtered_gene_bc_matrices.tar.gz<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u52a0\u8f7dPBMC\u6570\u636e\u96c6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span>.data <- Read10X(data.dir = <span class=\"code-snippet__string\">\".\/filtered_gene_bc_matrices\/hg19\/\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># Initialize the Seurat object with the raw (non-normalized data).<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span> <- CreateSeuratObject(counts = pbmc.data, project = <span class=\"code-snippet__string\">\"pbmc3k\"<\/span>, min.cells = 3, min.features = 200)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6570\u636e\u6807\u51c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span>[[<span class=\"code-snippet__string\">\"percent.mt\"<\/span>]] <- PercentageFeatureSet(pbmc, pattern = <span class=\"code-snippet__string\">\"^MT-\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># pbmc <- subset(pbmc, subset = nFeature_RNA > 200 & nFeature_RNA < 2500 & percent.mt < 5) # make some filtering based on QC metrics visualizations, see Seurat tutorial: https:\/\/satijalab.org\/seurat\/articles\/pbmc3k_tutorial.html<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span> <- NormalizeData(pbmc, normalization.method = <span class=\"code-snippet__string\">\"LogNormalize\"<\/span>, scale.factor = 10000)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span> <- FindVariableFeatures(pbmc, selection.method = <span class=\"code-snippet__string\">\"vst\"<\/span>, nfeatures = 2000) <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8fdb\u884cPCA\u5904\u7406<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span> <- ScaleData(pbmc, features = rownames(pbmc))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span> <- RunPCA(pbmc, features = VariableFeatures(object = pbmc))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u68c0\u67e5 PC \u7ec4\u4ef6\u7684\u6570\u91cf\uff08\u6839\u636e Elbow \u56fe\uff0c\u6211\u4eec\u9009\u62e9\u4e86 10 \u4e2a PC \u8fdb\u884c\u4e0b\u6e38\u5206\u6790\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">ElbowPlot<\/span>(pbmc)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u805a\u7c7b\u548c\u53ef\u89c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span> <- FindNeighbors(pbmc, dims = 1:10)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span> <- FindClusters(pbmc, resolution = 0.8)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span> <- RunUMAP(pbmc, dims = 1:10)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">DimPlot<\/span>(pbmc, reduction = <span class=\"code-snippet__string\">\"umap\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(47, 85, 151);font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">          <o:p> <\/o:p><\/span><span style=\"font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">    <\/span><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100016480\" data-ratio=\"0.8385321100917431\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_t5GGQMpKp73ggZc38QXuJjbt70NhmA.png?resize=544%2C456\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzwiazfwqaFEHgqLAZibbKOhBia8qKowL1fSwWMH9pyic6icDYGJt5GGQMpKp73ggZc38QXuJjbt70NhmA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"545\" height=\"456 .739990234375\" style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 1px;\" width=\"544 .739990234375\" data-recalc-dims=\"1\" \/><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 1px;\">   <\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 1px;\">  <\/span><o:p style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 1px;\"> <\/o:p><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(47, 85, 151);font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">##\u7ec6\u80de\u7c7b\u578b\u6ce8\u91ca<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u4f7f\u7528 ScType \u81ea\u52a8\u5206\u914d\u5355\u5143\u683c\u7c7b\u578b\u3002\u9996\u5148\u52a0\u8f7d\u4e24\u4e2a\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f7d\u5165\u57fa\u56e0\u7ec4\u6784\u5efa\u51fd\u6570<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">source<\/span>(<span class=\"code-snippet__string\">\"https:\/\/raw.githubusercontent.com\/IanevskiAleksandr\/sc-type\/master\/R\/gene_sets_prepare.R\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u52a0\u8f7d\u7ec6\u80de\u7c7b\u578b\u6ce8\u91ca<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__built_in\">source<\/span>(<span class=\"code-snippet__string\">\"https:\/\/raw.githubusercontent.com\/IanevskiAleksandr\/sc-type\/master\/R\/sctype_score_.R\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(47, 85, 151);font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u63a5\u4e0b\u6765\uff0c\u8ba9\u6211\u4eec\u4ece\u8f93\u5165\u7ec6\u80de\u6807\u8bb0\u6587\u4ef6\u4e2d\u51c6\u5907\u57fa\u56e0\u96c6\u3002<\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;font-variant: normal;text-transform: none;\">\u9ed8\u8ba4\u60c5\u51b5\u4e0b\uff0c\u6211\u4eec\u4f7f\u7528\u5185\u7f6e\u7684\u5355\u5143\u683c\u6807\u8bb0\u6570\u636e\u5e93\uff0c\u4f46\u662f\uff0c\u8bf7\u968f\u610f\u4f7f\u7528\u60a8\u81ea\u5df1\u7684\u6570\u636e\u3002<\/span>    <\/span><o:page><\/o:page><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u53ea\u9700\u51c6\u5907\u4e00\u4e2a\u4e0e\u6211\u4eec\u7684\u6570\u636e\u5e93\u6587\u4ef6\u76f8\u540c\u683c\u5f0f\u7684\u8f93\u5165 XLSX \u6587\u4ef6<\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u6570\u636e\u5e93\u6587\u4ef6\u5e94\u5305\u542b\u56db\u5217\uff08\u7ec4\u7ec7\u7c7b\u578b – \u7ec4\u7ec7\u7c7b\u578b\uff0c\u5355\u5143\u683c\u540d\u79f0 – \u7ec6\u80de\u7c7b\u578b\uff0cgeneSymbolmore1 – \u9633\u6027\u6807\u8bb0\u57fa\u56e0\uff0cgeneSymbolmore2 – \u9884\u8ba1\u4e0d\u4f1a\u7531\u7ec6\u80de\u7c7b\u578b\u8868\u8fbe\u7684\u6807\u8bb0\u57fa\u56e0\uff09<\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u6700\u540e\uff0c\u8ba9\u6211\u4eec\u4e3a\u6bcf\u4e2a\u805a\u7c7b\u5206\u914d\u7ec6\u80de\u7c7b\u578b<\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(47, 85, 151);font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#DB file<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">db_ = <span class=\"code-snippet__string\">\"https:\/\/raw.githubusercontent.com\/IanevskiAleksandr\/sc-type\/master\/ScTypeDB_full.xlsx\"<\/span>;<\/span><\/code><code><span class=\"code-snippet_outer\">tissue = <span class=\"code-snippet__string\">\"Immune system\"<\/span> <span class=\"code-snippet__comment\"># e.g. Immune system,Pancreas,Liver,Eye,Kidney,Brain,Lung,Adrenal,Heart,Intestine,Muscle,Placenta,Spleen,Stomach,Thymus <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># prepare gene sets<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">gs_list = gene_sets_prepare(db_, tissue)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># prepare gene sets<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">gs_list = gene_sets_prepare(db_, tissue)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># get cell-type by cell matrix<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">es.max = sctype_score(scRNAseqData = pbmc[[<span class=\"code-snippet__string\">\"RNA\"<\/span>]]@scale.data, scaled = TRUE, <\/span><\/code><code><span class=\"code-snippet_outer\"> gs = gs_list$gs_positive, gs2 = gs_list$gs_negative)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(47, 85, 151);font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"> <\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;font-variant: normal;text-transform: none;\">\u6ce8\u610f\uff1ascRNAseqData \u53c2\u6570\u5e94\u4e0e\u8f93\u5165\u7684 scRNA-seq \u77e9\u9635\u76f8\u5bf9\u5e94\u3002<\/span>    <\/span><o:page><\/o:page><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u5982\u679c\u4f7f\u7528 Seurat\uff0c\u5219\u4e3a pbmc[[“RNA”]]@scale.data\uff08\u9ed8\u8ba4\u503c\uff09\uff1b\u5982\u679c\u4f7f\u7528 sctransform \u8fdb\u884c\u5f52\u4e00\u5316\uff0c\u5219\u4e3a pbmc[[“SCT”]]@scale.data\u3001<\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u6216 pbmc[[“integrated”]]@scale.data\uff08\u5bf9\u591a\u4e2a\u5355\u7ec6\u80de\u6570\u636e\u96c6\u8fdb\u884c\u8054\u5408\u5206\u6790\u65f6\uff09<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 1px;\">        <\/span><o:p style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 1px;\"> <\/o:p><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(47, 85, 151);font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6309\u5206\u7fa4\u5408\u5e76<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cL_resutls = <span class=\"code-snippet__keyword\">do<\/span>.call(<span class=\"code-snippet__string\">\"rbind\"<\/span>, lapply(unique(pbmc@meta.data$seurat_clusters), <span class=\"code-snippet__function\"><span class=\"code-snippet__keyword\">function<\/span><span class=\"code-snippet__params\">(cl)<\/span><\/span>{<\/span><\/code><code><span class=\"code-snippet_outer\"> es.max.cl = sort(rowSums(es.max[ ,rownames(pbmc@meta.data[pbmc@meta.data$seurat_clusters==cl, ])]), decreasing = !<span class=\"code-snippet__number\">0<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> head(data.frame(cluster = cl, type = names(es.max.cl), scores = es.max.cl, ncells = sum(pbmc@meta.data$seurat_clusters==cl)), <span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">}))<\/span><\/code><code><span class=\"code-snippet_outer\">sctype_scores = cL_resutls %>% group_by(cluster) %>% top_n(n = <span class=\"code-snippet__number\">1<\/span>, wt = scores) <\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u5c06\u4f4e\u7f6e\u4fe1\u5ea6\uff08ScType \u5206\u6570\u4f4e\uff09\u7fa4\u8bbe\u4e3a \"unknown\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">sctype_scores$type[<span class=\"code-snippet__keyword\">as<\/span>.numeric(<span class=\"code-snippet__keyword\">as<\/span>.character(sctype_scores$scores)) < sctype_scores$ncells\/<span class=\"code-snippet__number\">4<\/span>] = <span class=\"code-snippet__string\">\"Unknown\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">print<\/span>(sctype_scores[,<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">3<\/span>])<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(47, 85, 151);font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;font-variant: normal;text-transform: none;\">\u7ed3\u679c<\/span>    <\/span><o:page><\/o:page><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100016481\" data-ratio=\"0.6142131979695431\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_jDKLOIYCgBqKjWV6pDeCiapJvFCibg.png?resize=394%2C242\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzwiazfwqaFEHgqLAZibbKOhBs3T1A7rIWf17eWkW17Rxf2HQGrjDKLOIYCgBqKjWV6pDeCiapJvFCibg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"394\" height=\"242 .0\" width=\"394 .010009765625\" data-recalc-dims=\"1\" \/><\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">sctype_score \u51fd\u6570\u901a\u8fc7 gs \u548c gs2\u53c2\u6570\u63a5\u53d7\u6b63\u6807\u8bb0\u548c\u8d1f\u6807\u8bb0\u3002\u5982\u679c\u6ca1\u6709\u8d1f\u6807\u8bb0(\u5373\u6807\u8bb0\u63d0\u4f9b\u8bc1\u636e\u8bc1\u660e\u7ec6\u80de\u5177\u6709\u7279\u5b9a\u7684\u7ec6\u80de\u7c7b\u578b) \uff0c\u53ea\u9700\u5c06 gs2\u53c2\u6570\u8bbe\u7f6e\u4e3a NULL (\u5373gs2 = NULL)\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 1px;\">        <\/span><o:p style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 1px;\"> <\/o:p><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(47, 85, 151);font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"ruby\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">##\u53ef\u89c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u5c06\u8bc6\u522b\u7684\u7ec6\u80de\u7c7b\u578b\u53e0\u52a0\u5728 UMAP \u56fe\u4e0a<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc@meta.data$customclassif = <span class=\"code-snippet__string\">\"\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(j <span class=\"code-snippet__keyword\">in<\/span> unique(sctype_scores$cluster)){<\/span><\/code><code><span class=\"code-snippet_outer\"> cl_type = sctype_scores[sctype_scores$cluster==j,]; <\/span><\/code><code><span class=\"code-snippet_outer\"> pbmc@meta.data$customclassif[pbmc@meta.data$seurat_clusters == j] = as.character(cl_type$type[<span class=\"code-snippet__number\">1<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\">DimPlot(pbmc, reduction = <span class=\"code-snippet__string\">\"umap\"<\/span>, label = TRUE, repel = TRUE, group.by = <span class=\"code-snippet__string\">'customclassif'<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><br \/><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100016482\" data-ratio=\"0.7591362126245847\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_9CfmuZ19LpGjXqbbarwLavgDp76icg.png?resize=553%2C420\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzwiazfwqaFEHgqLAZibbKOhBjEuJpPiaSTolM0Y1xic58MCX6HU9CfmuZ19LpGjXqbbarwLavgDp76icg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"602\" height=\"420 .3399658203125\" width=\"553 .47998046875\" data-recalc-dims=\"1\" \/><\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u6b64\u5916\uff0c\u53ef\u4ee5\u53ef\u89c6\u5316\u4e00\u4e2a\u6c14\u6ce1\u56fe\uff0c\u663e\u793a ScType \u8003\u8651\u7528\u4e8e\u805a\u7c7b\u6ce8\u91ca\u7684\u6240\u6709\u7ec6\u80de\u7c7b\u578b\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 1px;\">         <\/span><o:p style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 1px;\"> <\/o:p><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(47, 85, 151);font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u52a0\u8f7dR\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">lapply(c(<span class=\"code-snippet__string\">\"ggraph\"<\/span>,<span class=\"code-snippet__string\">\"igraph\"<\/span>,<span class=\"code-snippet__string\">\"tidyverse\"<\/span>, <span class=\"code-snippet__string\">\"data.tree\"<\/span>), library, character.only = T)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u7ed8\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">cL_resutls=cL_resutls[order(cL_resutls$cluster),]; edges = cL_resutls; edges$type = paste<span class=\"code-snippet__number\">0<\/span>(edges$type,<span class=\"code-snippet__string\">\"_\"<\/span>,edges$cluster); edges$cluster = paste<span class=\"code-snippet__number\">0<\/span>(<span class=\"code-snippet__string\">\"cluster \"<\/span>, edges$cluster); edges = edges[,c(<span class=\"code-snippet__string\">\"cluster\"<\/span>, <span class=\"code-snippet__string\">\"type\"<\/span>)]; colnames(edges) = c(<span class=\"code-snippet__string\">\"from\"<\/span>, <span class=\"code-snippet__string\">\"to\"<\/span>); rownames(edges) <- NULL<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\">nodes_lvl1 = sctype_scores[,c(<span class=\"code-snippet__string\">\"cluster\"<\/span>, <span class=\"code-snippet__string\">\"ncells\"<\/span>)]; nodes_lvl1$cluster = paste<span class=\"code-snippet__number\">0<\/span>(<span class=\"code-snippet__string\">\"cluster \"<\/span>, nodes_lvl1$cluster); nodes_lvl1$Colour = <span class=\"code-snippet__string\">\"#f1f1ef\"<\/span>; nodes_lvl1$ord = <span class=\"code-snippet__number\">1<\/span>; nodes_lvl1$realname = nodes_lvl1$cluster; nodes_lvl1 = as.data.frame(nodes_lvl1); nodes_lvl2 = c(); <\/span><\/code><code><span class=\"code-snippet_outer\">ccolss= c(<span class=\"code-snippet__string\">\"#5f75ae\"<\/span>,<span class=\"code-snippet__string\">\"#92bbb8\"<\/span>,<span class=\"code-snippet__string\">\"#64a841\"<\/span>,<span class=\"code-snippet__string\">\"#e5486e\"<\/span>,<span class=\"code-snippet__string\">\"#de8e06\"<\/span>,<span class=\"code-snippet__string\">\"#eccf5a\"<\/span>,<span class=\"code-snippet__string\">\"#b5aa0f\"<\/span>,<span class=\"code-snippet__string\">\"#e4b680\"<\/span>,<span class=\"code-snippet__string\">\"#7ba39d\"<\/span>,<span class=\"code-snippet__string\">\"#b15928\"<\/span>,<span class=\"code-snippet__string\">\"#ffff99\"<\/span>, <span class=\"code-snippet__string\">\"#6a3d9a\"<\/span>,<span class=\"code-snippet__string\">\"#cab2d6\"<\/span>,<span class=\"code-snippet__string\">\"#ff7f00\"<\/span>,<span class=\"code-snippet__string\">\"#fdbf6f\"<\/span>,<span class=\"code-snippet__string\">\"#e31a1c\"<\/span>,<span class=\"code-snippet__string\">\"#fb9a99\"<\/span>,<span class=\"code-snippet__string\">\"#33a02c\"<\/span>,<span class=\"code-snippet__string\">\"#b2df8a\"<\/span>,<span class=\"code-snippet__string\">\"#1f78b4\"<\/span>,<span class=\"code-snippet__string\">\"#a6cee3\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span> (i in <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__keyword\">length<\/span>(unique(cL_resutls$cluster))){<\/span><\/code><code><span class=\"code-snippet_outer\"> dt_tmp = cL_resutls[cL_resutls$cluster == unique(cL_resutls$cluster)[i], ]; nodes_lvl2 = rbind(nodes_lvl2, data.frame(cluster = paste<span class=\"code-snippet__number\">0<\/span>(dt_tmp$type,<span class=\"code-snippet__string\">\"_\"<\/span>,dt_tmp$cluster), ncells = dt_tmp$scores, Colour = ccolss[i], <span class=\"code-snippet__keyword\">ord<\/span> = <span class=\"code-snippet__number\">2<\/span>, realname = dt_tmp$type))<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">nodes = rbind(nodes_lvl1, nodes_lvl2); nodes$ncells[nodes$ncells<<span class=\"code-snippet__number\">1<\/span>] = <span class=\"code-snippet__number\">1<\/span>;<\/span><\/code><code><span class=\"code-snippet_outer\">files_db = openxlsx::read.xlsx(db<span class=\"code-snippet__number\">_<\/span>)[,c(<span class=\"code-snippet__string\">\"cellName\"<\/span>,<span class=\"code-snippet__string\">\"shortName\"<\/span>)]; files_db = unique(files_db); nodes = merge(nodes, files_db, all.x = T, all.y = F, by.x = <span class=\"code-snippet__string\">\"realname\"<\/span>, by.y = <span class=\"code-snippet__string\">\"cellName\"<\/span>, <span class=\"code-snippet__keyword\">sort<\/span> = F)<\/span><\/code><code><span class=\"code-snippet_outer\">nodes$shortName[is.na(nodes$shortName)] = nodes$realname[is.na(nodes$shortName)]; nodes = nodes[,c(<span class=\"code-snippet__string\">\"cluster\"<\/span>, <span class=\"code-snippet__string\">\"ncells\"<\/span>, <span class=\"code-snippet__string\">\"Colour\"<\/span>, <span class=\"code-snippet__string\">\"ord\"<\/span>, <span class=\"code-snippet__string\">\"shortName\"<\/span>, <span class=\"code-snippet__string\">\"realname\"<\/span>)] <\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\">mygraph <- graph_from_data_frame(edges, vertices=nodes)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\">gggr<- ggraph(mygraph, layout = <span class=\"code-snippet__string\">'circlepack'<\/span>, weight=I(ncells)) + <\/span><\/code><code><span class=\"code-snippet_outer\"> geom_node_circle(aes(filter=<span class=\"code-snippet__keyword\">ord<\/span>==<span class=\"code-snippet__number\">1<\/span>,fill=I(<span class=\"code-snippet__string\">\"#F5F5F5\"<\/span>), colour=I(<span class=\"code-snippet__string\">\"#D3D3D3\"<\/span>)), alpha=<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">9<\/span>) + geom_node_circle(aes(filter=<span class=\"code-snippet__keyword\">ord<\/span>==<span class=\"code-snippet__number\">2<\/span>,fill=I(Colour), colour=I(<span class=\"code-snippet__string\">\"#D3D3D3\"<\/span>)), alpha=<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">9<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> theme_void() + geom_node_text(aes(filter=<span class=\"code-snippet__keyword\">ord<\/span>==<span class=\"code-snippet__number\">2<\/span>, label=shortName, colour=I(<span class=\"code-snippet__string\">\"#ffffff\"<\/span>), fill=<span class=\"code-snippet__string\">\"white\"<\/span>, repel = !<span class=\"code-snippet__number\">1<\/span>, parse = T, size = I(<span class=\"code-snippet__keyword\">log<\/span>(ncells,<span class=\"code-snippet__number\">25<\/span>)*<span class=\"code-snippet__number\">1.5<\/span>)))+ geom_node_label(aes(filter=<span class=\"code-snippet__keyword\">ord<\/span>==<span class=\"code-snippet__number\">1<\/span>, label=shortName, colour=I(<span class=\"code-snippet__string\">\"#000000\"<\/span>), size = I(<span class=\"code-snippet__number\">3<\/span>), fill=<span class=\"code-snippet__string\">\"white\"<\/span>, parse = T), repel = !<span class=\"code-snippet__number\">0<\/span>, segment.linetype=<span class=\"code-snippet__string\">\"dotted\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\">scater::multiplot(DimPlot(pbmc, reduction = <span class=\"code-snippet__string\">\"umap\"<\/span>, label = TRUE, repel = TRUE, cols = ccolss), gggr, cols = <span class=\"code-snippet__number\">2<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(47, 85, 151);font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">          <o:p> <\/o:p><\/span><span style=\"font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">    <\/span><o:page><\/o:page><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100016485\" data-ratio=\"0.5320139697322468\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_9Lh058zP8THCPI7LWvROzibyFZ1btQ.png?resize=553%2C294\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAzwiazfwqaFEHgqLAZibbKOhBDbSr9VhFM0wlhv5Z8XuV705299Lh058zP8THCPI7LWvROzibyFZ1btQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"859\" height=\"294 .5400085449219\" width=\"553 .47998046875\" data-recalc-dims=\"1\" \/><\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">          <o:p> <\/o:p><\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-weight: bold;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u5c0f\u7ed3<\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u7ec6\u80de\u7c7b\u578b\u6ce8\u91ca\u5bf9\u4e8e\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u81f3\u5173\u91cd\u8981\uff0c\u4f46\u4ee5\u524d\u7684\u65b9\u6cd5\u5f80\u5f80\u7e41\u7410\u800c\u8017\u65f6\u3002ScType\u7684\u51fa\u73b0\u6539\u53d8\u4e86\u8fd9\u4e00\u73b0\u72b6\uff0c\u63d0\u4f9b\u4e86\u4e00\u79cd\u9ad8\u6548\u4e14\u7cbe\u786e\u7684\u66ff\u4ee3\u65b9\u6848\u3002\u73b0\u5728\u53ea\u9700\u63d0\u4f9b\u6570\u636e\uff0cScType\u5c06\u4e3a\u60a8\u63d0\u4f9b\u53ef\u9760\u7684\u7ec6\u80de\u7c7b\u578b\u6ce8\u91ca\u3002\u8fd9\u5c06\u6781\u5927\u5730\u52a0\u901f\u7814\u7a76\u8fdb\u7a0b\uff0c\u540c\u65f6\u63d0\u4f9b\u66f4\u6df1\u5165\u7684\u6d1e\u5bdf\u529b\u3002\u5feb\u8ddf\u968f\u5c0f\u679c\u7684\u811a\u6b65\u653e\u624b\u4e00\u8bd5\u5427\uff0cScType\u5c06\u5e2e\u52a9\u60a8\u6df1\u5165\u63a2\u7d22\u5355\u7ec6\u80de\u6570\u636e\u4e2d\u7684\u7ec6\u80de\u591a\u6837\u6027\u3002<\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-size: 16px;font-variant: normal;text-transform: none;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u7528ScType\uff0c\u91ca\u653e\u4f60\u7684\u79d1\u7814\u6f5c\u529b\uff0c\u6df1\u5165\u4e86\u89e3\u7ec6\u80de\u7c7b\u578b\u7684\u7cbe\u5f69\u4e16\u754c\uff01\u4ee3\u7801\u57fa\u7840\u8584\u5f31\u7684\u540c\u5b66\u4e5f\u522b\u62c5\u5fc3\u4e0d\u4f1a\u5bf9\u5355\u7ec6\u80de\u6570\u636e\u8fdb\u884c\u6ce8\u91ca\u54e6\uff01\u4e0d\u59a8\u6765\u4f53\u9a8c\u4e00\u4e0b\u6211\u4eec\u76840\u4ee3\u7801\u751f\u4fe1\u5206\u6790\u5e73\u53f0\u5427\uff01\u5c0f\u679c\u4eb2\u81ea\u4e3a\u4f60\u5448\u4e0a\u7f51\u5740\uff1a<\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">http:\/\/www.biocloudservice.com\/home.html<\/span><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u3002<\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u4f60\u60f3\u8981\u7684\u5206\u6790\u5c0f\u679c\u8fd9\u91cc\u90fd\u80fd\u7ed9\u4f60\u63d0\u4f9b\u54e6\uff01\uff01<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;font-weight: bold;letter-spacing: 1px;\">        <\/span><o:p style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;font-weight: bold;letter-spacing: 1px;\"> <\/o:p><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-variant: normal;text-transform: none;font-weight: bold;font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\">\u53c2\u8003\u6587\u732e<\/span><\/section>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><span style=\"font-size: 16px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 1px;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;font-variant: normal;text-transform: none;\">Ianevski A, Giri AK, Aittokallio T. Fully-automated and ultra-fast cell-type identification using specific marker combinations from single-cell transcriptomic data. Nat Commun. 2022;13(1):1246.<\/span>    <\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 1px;\"> <\/span><\/section>\n<p style=\"text-indent: 0px;margin-bottom: 0px;\"><span style=\"color: rgb(0, 122, 170);\"><strong><span style=\"color: rgb(0, 122, 170);letter-spacing: 0.578px;text-indent: 32px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 15px;\">\u5c0f\u679c\u8fd8<span style=\"color: rgb(0, 122, 170);background-color: rgb(255, 255, 255);font-size: 15px;letter-spacing: 0.578px;text-decoration: none solid rgba(0, 0, 0, 0.9);\">\u63d0\u4f9b\u601d\u8def\u8bbe\u8ba1\u3001\u5b9a\u5236\u751f\u4fe1\u5206\u6790\u3001\u6587\u732e\u601d\u8def\u590d\u73b0\uff1b\u6709\u9700\u8981\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u76f4\u63a5\u626b\u7801\u54a8\u8be2\u5c0f\u679c\uff0c\u7aed\u8bda\u4e3a\u60a8\u7684\u79d1\u7814\u52a9\u529b\uff01<\/span><\/span><\/strong><\/span><\/p>\n<p 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0;width: 75%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100016487\" data-ratio=\"1\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_B2jkicacLzPjw4xMSqVvoucZjonVDA-4.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxVYfSsM0wdf9d3RpNiaTcHohxPNEVs2VFhPMukpU8lchxrvcB2jkicacLzPjw4xMSqVvoucZjonVDA\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"430\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 56.9106%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"line-height: 2;letter-spacing: 1px;color: rgb(43, 65, 110);\" powered-by=\"xiumi.us\">\n<p style=\"margin-bottom: 2px;\"><strong>\u5b9a\u5236\u751f\u4fe1\u5206\u6790<\/strong><\/p>\n<p style=\"margin-bottom: 2px;\"><strong>\u670d\u52a1\u5668\u79df\u8d41<\/strong><\/p>\n<p><strong>\u626b\u7801\u54a8\u8be2\u5c0f\u679c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497780&idx=2&sn=7eee90af6f387e4153bba13028e545a1&chksm=cf7f68f1f808e1e7dfb3a0597ef442e5c6e700ecf6ee5a035c766a4c7486c12eabd0ab218ea6&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" 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94);line-height: 1;letter-spacing: 5px;text-align: center;\">\n<p><strong>\u5f80\u671f\u56de\u987e<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"height: 3px;background-color: rgb(95, 156, 239);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: 2px;margin-right: 0%;margin-left: 0%;text-align: center;line-height: 0.8;font-size: 9px;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;border-top: 0.8em solid rgb(58, 66, 94);border-left: 0.7em solid transparent !important;border-right: 0.7em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;flex: 100 100 0%;align-self: flex-end;height: auto;\">\n<section style=\"margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"height: 3px;background-color: rgb(95, 156, 239);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: 2px;margin-right: 0%;margin-left: 0%;text-align: center;line-height: 0.8;font-size: 9px;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;border-top: 0.8em solid rgba(255, 255, 255, 0);border-left: 0.7em solid transparent !important;border-right: 0.7em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"min-height: 40px;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"width: 100%;margin-right: auto;margin-bottom: -10px;margin-left: auto;\">\n<table width=\"100%\">\n<tbody>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:0\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:0.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;box-shadow: rgb(0, 0, 0) 0px 0px 0px;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>01<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497780&idx=2&sn=7eee90af6f387e4153bba13028e545a1&chksm=cf7f68f1f808e1e7dfb3a0597ef442e5c6e700ecf6ee5a035c766a4c7486c12eabd0ab218ea6&scene=21#wechat_redirect\" textvalue=\"1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff0c\u4e24\u4eba\u6210\u56e2\uff0c1\u4eba\u514d\u5355\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff0c\u4e24\u4eba\u6210\u56e2\uff0c1\u4eba\u514d\u5355\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:1\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:1.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>02<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498878&idx=1&sn=fc34ed3f1d15d060bbaf8166adcebd58&chksm=cf7f6cbbf808e5ad0b5417e50ed75cfbd1b3a83ac43b97f1dfa718c822878a9a004990075133&scene=21#wechat_redirect\" textvalue=\"\u5355\u4e2a\u6570\u636e\u5e93\u7528\u817b\u4e86\uff1f\u591a\u6570\u636e\u5e93\u201c\u7ec4\u5408\u62f3\u201d\u5e26\u4f60\u6253\u5f00\u514d\u75ab\u6d78\u6da6\u65b0\u601d\u8def\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u5355\u4e2a\u6570\u636e\u5e93\u7528\u817b\u4e86\uff1f\u591a\u6570\u636e\u5e93\u201c\u7ec4\u5408\u62f3\u201d\u5e26\u4f60\u6253\u5f00\u514d\u75ab\u6d78\u6da6\u65b0\u601d\u8def\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:2\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:2.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>03<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498673&idx=1&sn=1e09b74a836c1e64a62ae1f66eeb3f93&chksm=cf7f6b74f808e262de70be43fd73bf5fefdc0914e525eb2032f741a0152e619689cedc76946f&scene=21#wechat_redirect\" textvalue=\"\u5b5f\u5fb7\u5c14\u968f\u673a\u5316\u7684\u51c6\u5907\u5de5\u4f5c\uff0cGWAS\u6570\u636e\u7684\u7f51\u7ad9\u4e0b\u8f7d\u65b9\u6cd5\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"11\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u5b5f\u5fb7\u5c14\u968f\u673a\u5316\u7684\u51c6\u5907\u5de5\u4f5c\uff0cGWAS\u6570\u636e\u7684\u7f51\u7ad9\u4e0b\u8f7d\u65b9\u6cd5<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:3\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:3.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>04<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498517&idx=1&sn=fa3c0104cee316982fe8162b79fe234f&chksm=cf7f6bd0f808e2c6cf979704bf883cfb5480444604f90da61dc286e7541fa5bb49df0a2fb58a&scene=21#wechat_redirect\" textvalue=\"\u8ddf\u7740\u5c0f\u679c\u5b66\u590d\u73b0-\u624b\u628a\u624b\u5e26\u4f60\u62ff\u4e0bIF=46.9Nature \u7ea7\u522b\u7684\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u56fe\uff01\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u8ddf\u7740\u5c0f\u679c\u5b66\u590d\u73b0-\u624b\u628a\u624b\u5e26\u4f60\u62ff\u4e0bIF=46.9Nature \u7ea7\u522b\u7684\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u56fe\uff01\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: center;margin-bottom: 0px;\"><br \/><\/p>\n<section style=\"text-align: justify;margin-top: 8px;margin-bottom: 24px;line-height: 1.6em;\"><o:page><\/o:page><\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5c0f\u679c\u5e26\u4f60\u73a9\u8f6c\u5355\u7ec6\u80de\u6ce8\u91ca\u5de5\u5177-ScType \u5e08\u59b9  \u751f\u4fe1\u679c  2024- […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58100"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=58100"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58100\/revisions"}],"predecessor-version":[{"id":58111,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58100\/revisions\/58111"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=58100"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=58100"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=58100"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}