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data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"Volcano plot\u2014\u2014\u522b\u518d\u95ee\u6211\u8fd9\u4e3a\u4ec0\u4e48\u662f\u706b\u5c71\u56fe\",\"time\":\"2023-07-13 19:00:16\",\"timeStamp\":\"1689246016\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247490042&idx=3&sn=3fe6c12e4097dc6e4e91cf53458153a2&chksm=cf7c893ff80b0029343c53f081b504a4b5708b6c1b3f82723d28df751247a49e19e69a3f6c73#rd\" target=\"_blank\" rel=\"noopener\">Volcano plot\u2014\u2014\u522b\u518d\u95ee\u6211\u8fd9\u4e3a\u4ec0\u4e48\u662f\u706b\u5c71\u56fe<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u56fe <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" 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style=\"font-size: 17px;color: rgb(255, 255, 255);text-align: right;letter-spacing: 4px;\" powered-by=\"xiumi.us\">\n<p><strong>\u70b9\u51fb\u84dd\u5b57<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;border-left: 4px solid rgb(242, 183, 183);border-bottom-left-radius: 0px;flex: 100 100 0%;align-self: stretch;height: auto;padding: 3px 10px;background-color: rgba(254, 245, 240, 0.8);\">\n<section style=\"color: rgb(181, 126, 121);font-size: 17px;letter-spacing: 4px;\" powered-by=\"xiumi.us\">\n<p><strong>\u5173\u6ce8\u5c0f\u56fe<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">\u4eca\u5929\u5c0f\u56fe\u751f\u4fe1\u9910\u9986\u7ed9\u5c0f\u4f19\u4f34\u63a8\u8350\u9053\u751f\u4fe1\u9910\uff0c\u80fd\u91cf\u8f7b\u9910\uff1a\u201c\u706b\u5c71\u5305\u201d<\/p>\n<p style=\"text-indent: 2em;white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u80fd\u91cf\u6e90\u4e8e\u771f\u6750\u5b9e\u6599<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">\u706b\u5c71\u56fe\uff08Volcano plot\uff09\u662f\u4e00\u79cd\u6bd4\u8f83\u201c\u8fdc\u53e4\u201d\u7684\u4e00\u79cd\u6563\u70b9\u56fe\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u57fa\u56e0\u7ec4\u3001\u8f6c\u5f55\u7ec4\u3001\u86cb\u767d\u7ec4\u7b49\u7ec4\u95f4\u5dee\u5f02\u5206\u6790\u7ed3\u679c\u7684\u5c55\u793a\u3002\u56fe\u8868\u7684\u6a2a\u8f74\u4e00\u822c\u5c55\u793a\u5dee\u5f02\u500d\u6570\u7684\u53d8\u5316\uff0c\u800c\u7eb5\u8f74\u8868\u793a\u5dee\u5f02\u5206\u6790\u7ed3\u679c\u7684\u53ef\u9760\u6027\u3002\u901a\u8fc7\u627e\u5230\u663e\u8457\u5dee\u5f02\u7684\u57fa\u56e0\uff0c\u7814\u7a76\u8005\u53ef\u4ee5\u8fdb\u4e00\u6b65\u505a\u540e\u7eed\u7684\u5b9e\u9a8c\u3002<\/p>\n<p style=\"text-indent: 2em;white-space: normal;\"><br \/><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u5728\u751f\u7269\u4fe1\u606f\u5206\u6790\u4e2d\uff0c\u706b\u5c71\u56fe\u662f\u975e\u5e38\u5e38\u89c1\u7684\u4e00\u79cd\u6570\u636e\u5c55\u793a\u5f62\u5f0f\u3002\u7531\u4e8e\u706b\u5c71\u56fe\u53ef\u4ee5\u975e\u5e38\u6e05\u6670\u7684\u5c55\u793a\u51fa\u54ea\u4e9b\u57fa\u56e0\u5728\u4e0d\u540c\u6837\u672c\u4e2d\u662f\u5177\u6709\u5dee\u5f02\u8868\u8fbe\u663e\u8457\u6027\u7684\u57fa\u56e0\uff0c\u56e0\u6b64\u5728\u751f\u7269\u533b\u5b66\u4e2d\uff0c\u5e38\u5e94\u7528\u4e8e\u75c5\u4f8b\u548c\u5bf9\u7167\u7ec4\u7684\u8f6c\u5f55\u7ec4\u7814\u7a76\uff0c\u4e5f\u80fd\u5e94\u7528\u4e8e\u57fa\u56e0\u7ec4\uff0c\u86cb\u767d\u8d28\u7ec4\uff0c\u4ee3\u8c22\u7ec4\u7b49\u7edf\u8ba1\u6570\u636e\u3002<\/p>\n<p style=\"text-indent: 2em;white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u8f7b\u98df\u83dc\u5355<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u5e38\u89c1\u706b\u5c71\u56fe\u7c7b\u578b\uff1a<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.002409638554217\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_JqfnBibcQaOW1767zReRL1icsSPdqA-1.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QvJwJT1YzjHzVO1g6SoONncKW7bHZLO6cvLMDc1ibw7KX5mlzCJqfnBibcQaOW1767zReRL1icsSPdqA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"415\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.0192771084337349\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_K9IxYgKpY8VK1ribyvD0Z7SJMicBAg-1.png?w=640\" 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data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<ul style=\"list-style-type: \uf06c;padding-left: 40px;list-style-position: outside;\" class=\"list-paddingleft-1\" powered-by=\"xiumi.us\">\n<li>\n<p>\u6bcf\u4e2a\u70b9\u4ee3\u8868\u4e00\u4e2a\u68c0\u6d4b\u5230\u7684\u57fa\u56e0\u3002<\/p>\n<\/li>\n<li>\n<p>\u6a2a\u8f74\u548c\u7eb5\u8f74\u7528\u4e8e\u56fa\u5b9a\u70b9\u5728\u7a7a\u95f4\u7684\u4f4d\u7f6e\u3002<\/p>\n<\/li>\n<li>\n<p>\u4e00\u822c\u6a2a\u8f74\u662fLog2(fold change)\uff0c\u70b9\u8d8a\u504f\u79bb\u4e2d\u5fc3\uff0c\u8868\u793a\u5dee\u5f02\u500d\u6570\u8d8a\u5927\u3002<\/p>\n<\/li>\n<li>\n<p>\u7eb5\u8f74\u662f-Log 10 (pvalue)\uff0c\u70b9\u8d8a\u9760\u56fe\u7684\u9876\u90e8\u8868\u793a\u5dee\u5f02\u8d8a\u663e\u8457\u3002<\/p>\n<\/li>\n<li>\n<p>\u70b9\u7684\u5927\u5c0f\u548c\u989c\u8272\u4e5f\u53ef\u4ee5\u8868\u793a\u66f4\u591a\u7684\u5c5e\u6027\uff0c\u5982\u540e\u9762\u56fe\u4e2d\u70b9\u7684\u989c\u8272\u6807\u8bb0\u5176\u5bf9\u5e94\u7684\u57fa\u56e0\u662f\u4e0a\u8c03, \u4e0b\u8c03\u8fd8\u662f\u65e0\u5dee\u5f02\u3002<\/p>\n<\/li>\n<li>\n<p>\u5927\u5c0f\u4e5f\u53ef\u7528\u4e8e\u5c55\u793a\u57fa\u56e0\u8868\u8fbe\u7684\u5e73\u5747\u4e30\u5ea6\uff0c\u4e00\u822c\u6211\u4eec\u5173\u6ce8\u8868\u8fbe\u6c34\u5e73\u8f83\u9ad8\u4e14\u5dee\u5f02\u8f83\u5927\u7684\u57fa\u56e0\u7528\u4e8e\u540e\u7eed\u7684\u5206\u6790\u548c\u9a8c\u8bc1\u3002<\/p>\n<\/li>\n<\/ul>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u706b\u5c71\u56fe\u662f\u8bb8\u591a\u751f\u4fe1\u5e73\u53f0\u9996\u6279\u4e0a\u7ebf\u7684\u5e38\u89c1\u56fe\uff0c\u867d\u7136\u6211\u4eec\u7ecf\u8fc7\u4e86\u5927\u91cf\u4f18\u5316\uff0c\u589e\u52a0\u4e86\u8bf8\u591a\u53ef\u8c03\u53c2\u6570\uff1a\u989c\u8272\uff0c\u70b9\u5927\u5c0f\uff0c\u751a\u81f3legend\u7684\u4f4d\u7f6e\u548c\u987a\u5e8f\u90fd\u8fdb\u884c\u4e86\u4f18\u5316\uff0c\u4f46\u662f\uff0c\u8fd8\u662f\u4e0d\u80fd\u6ee1\u8db3\u5c0f\u4f19\u4f34\u7684\u9700\u6c42\uff1a<\/p>\n<p style=\"white-space: normal;\">\u80fd\u4e0d\u80fd\u6807\u6ce8\u6211\u611f\u5174\u8da3\u7684\u57fa\u56e0\uff1f<\/p>\n<p style=\"white-space: normal;\">\u80fd\u4e0d\u80fd\u7ed9\u70b9\u52a0\u4e0a\u900f\u660e\u5ea6\uff1f<\/p>\n<p style=\"white-space: normal;\">\u80fd\u4e0d\u80fd\u989d\u5916\u52a0\u4e9b\u7ebf\uff1f<\/p>\n<p style=\"white-space: normal;\">\u80fd\u4e0d\u80fd\u653e\u5927\u67d0\u4e9b\u70b9\uff1f<\/p>\n<p style=\"white-space: normal;\">\u80fd\u4e0d\u80fd\u628ap\u503c\u4e3a0\u7684\u70b9\u9608\u503c\u5bbd\u677e\u70b9\uff1f<\/p>\n<p style=\"white-space: normal;\">\u80fd\u4e0d\u80fd\u6807\u6ce8\u4e0b\u4e0a\u8c03\u591a\u5c11\uff0c\u4e0b\u8c03\u591a\u5c11\uff1f<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">\u5c0f\u56fe\u53ef\u4ee5\u8d1f\u8d23\u4efb\u5730\u544a\u8bc9\u5c0f\u4f19\u4f34\uff0c\u90fd\u80fd\u6ee1\u8db3\uff0cBut\uff0c\u4efb\u4f55\u5728\u7ebf\u5de5\u5177\u90fd\u4e0d\u80fd\u6ee1\u8db3\u60a8\u7684\u5b9a\u5236\u9700\u6c42\u3002\u8981\u60f3\u6ee1\u8db3\uff0c\u9700\u8981\u4f60\u81ea\u5df1\u7f16\u7a0b\u6216\u8005\u7f16\u8f91\u751f\u6210\u7684\u56fe\u3002<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u4eca\u5929\uff0c\u7ed9\u5c0f\u4f19\u4f34\u5e26\u6765\u7684\u5236\u4f5c\u6750\u6599\u662fRColorBrewer\u3001ggrepel\u3001ggplot R\u5305\u3002\u8fd9\u91cc\u5c0f\u56fe\u7ed9\u4e0d\u61c2\u7f16\u7a0b\u7684\u5c0f\u4f19\u4f34\u5e26\u6765\u4e0d\u4e00\u6837\u7684\u706b\u5c71\u56fe\u4f53\u9a8c\u3002\u8d70\u8d77\u2014\u2014\u2014\u2014<\/p>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u5236\u4f5c\u8fc7\u7a0b<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">rm(<span class=\"code-snippet__built_in\">list<\/span> = ls())#\u6e05\u9664\u6240\u6709\u7684\u53d8\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">library(RColorBrewer)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggrepel) #\u6807\u7b7e\u7528<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u63a5\u4e0b\u6765\u51c6\u5907\u4f5c\u56fe\u7684\u6570\u636e<br \/><\/p>\n<p style=\"white-space: normal;\">\u4eca\u5929\u7528\u5230\u7684geo\u6570\u636e\u662f\u7ecflimma\u5dee\u5f02\u5206\u6790\u540e\u7684\uff0c\u6570\u636e\u7684\u683c\u5f0f\u662f\u6587\u672ctext\uff0c\u53ef\u76f4\u63a5\u5bfc\u5165\u5230R\u4e2d\uff1a<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6105263157894737\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_tMma2iaLsf7OAZsuz6qNbuFTQibCHg-1.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QvJwJT1YzjHzVO1g6SoONnchdMVbYnZmHXYzcpiaf0oLrdSibibptMma2iaLsf7OAZsuz6qNbuFTQibCHg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"380\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">'D:\\limma'<\/span>)#\u52a0\u8f7d\u8def\u5f84<\/span><\/code><code><span class=\"code-snippet_outer\">df=read.table(<span class=\"code-snippet__string\">\"\u706b\u5c71\u56fe\u8f93\u5165\u6570\u636e.txt\"<\/span>,sep=<span class=\"code-snippet__string\">'t'<\/span>,header = T)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u786e\u5b9a\u662f\u4e0a\u8c03\u8fd8\u662f\u4e0b\u8c03\uff0c\u7528\u4e8e\u7ed9\u56fe\u4e2d\u70b9\u4e0a\u8272<\/span><\/code><code><span class=\"code-snippet_outer\">df$threshold = factor(ifelse(df$pvalue < <span class=\"code-snippet__number\">0.05<\/span> & <span class=\"code-snippet__built_in\">abs<\/span>(df$log2FoldChange) >= <span class=\"code-snippet__number\">0.25<\/span>, ifelse(df$log2FoldChange >= <span class=\"code-snippet__number\">0.25<\/span> ,<span class=\"code-snippet__string\">'Up'<\/span>,<span class=\"code-snippet__string\">'Down'<\/span>),<span class=\"code-snippet__string\">'NoSignifi'<\/span>),levels=c(<span class=\"code-snippet__string\">'Up'<\/span>,<span class=\"code-snippet__string\">'Down'<\/span>,<span class=\"code-snippet__string\">'NoSignifi'<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\">df$gene <- row.names(df) #\u6dfb\u52a0\u4e00\u5217\u57fa\u56e0\u540d\uff0c\u4ee5\u4fbf\u5907\u6ce8<\/span><\/code><code><span class=\"code-snippet_outer\">p <- ggplot(df,aes(x=log2FoldChange,y= -<span class=\"code-snippet__built_in\">log10<\/span>(pvalue),color=threshold))+<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(data = df[df$pvalue<<span class=\"code-snippet__number\">0.05<\/span>&<span class=\"code-snippet__built_in\">abs<\/span>(df$log2FoldChange)><span class=\"code-snippet__number\">0.25<\/span>,],size = <span class=\"code-snippet__number\">0.5<\/span>)+ <\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(data = df[df$pvalue><span class=\"code-snippet__number\">0.05<\/span>|<span class=\"code-snippet__built_in\">abs<\/span>(df$log2FoldChange)<<span class=\"code-snippet__number\">0.25<\/span>,],size = <span class=\"code-snippet__number\">0.5<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_color_manual(values=c(<span class=\"code-snippet__string\">\"#4393C3\"<\/span>,<span class=\"code-snippet__string\">\"#00000033\"<\/span>,<span class=\"code-snippet__string\">\"#FC4E2A\"<\/span>))+#\u786e\u5b9a\u70b9\u7684\u989c\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_text_repel(<\/span><\/code><code><span class=\"code-snippet_outer\"> data = df[df$pvalue<<span class=\"code-snippet__number\">0.05<\/span>&<span class=\"code-snippet__built_in\">abs<\/span>(df$fd)><span class=\"code-snippet__number\">1<\/span>,],<\/span><\/code><code><span class=\"code-snippet_outer\"> aes(label = gene),<\/span><\/code><code><span class=\"code-snippet_outer\"> size = <span class=\"code-snippet__number\">4.5<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> color = <span class=\"code-snippet__string\">\"black\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> segment.color = <span class=\"code-snippet__string\">\"black\"<\/span>, show.legend = FALSE )+#\u6dfb\u52a0\u5173\u6ce8\u7684\u70b9\u7684\u57fa\u56e0\u540d<\/span><\/code><code><span class=\"code-snippet_outer\"> ylab(<span class=\"code-snippet__string\">'-log10 (P_value)'<\/span>)+#\u4fee\u6539y\u8f74\u540d\u79f0<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab(<span class=\"code-snippet__string\">'log2 (FoldChange)'<\/span>)+#\u4fee\u6539x\u8f74\u540d\u79f0<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_vline(xintercept=c(<span class=\"code-snippet__number\">-0.25<\/span>,<span class=\"code-snippet__number\">0.25<\/span>),lty=<span class=\"code-snippet__number\">3<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.5<\/span>) +#\u6dfb\u52a0\u6a2a\u7ebf|logFoldChange|><span class=\"code-snippet__number\">0.25<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> geom_hline(yintercept = -<span class=\"code-snippet__built_in\">log10<\/span>(<span class=\"code-snippet__number\">0.05<\/span>),lty=<span class=\"code-snippet__number\">3<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.5<\/span>) +#\u6dfb\u52a0\u7ad6\u7ebfpadj<<span class=\"code-snippet__number\">0.05<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> theme_classic( # \u4e3b\u9898\u8bbe\u7f6e\uff0c\u8fd9\u4e2a\u662f\u65e0\u7ebf\u6761\u4e3b\u9898<\/span><\/code><code><span class=\"code-snippet_outer\"> base_line_size = <span class=\"code-snippet__number\">1<\/span> # \u5750\u6807\u8f74\u7684\u7c97\u7ec6<\/span><\/code><code><span class=\"code-snippet_outer\"> )+<\/span><\/code><code><span class=\"code-snippet_outer\"> theme(axis.title.x = element_text(size = <span class=\"code-snippet__number\">15<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> color = <span class=\"code-snippet__string\">\"black\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> face = <span class=\"code-snippet__string\">\"bold\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.title.y = element_text(size = <span class=\"code-snippet__number\">15<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> color = <span class=\"code-snippet__string\">\"black\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> face = <span class=\"code-snippet__string\">\"bold\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> vjust = <span class=\"code-snippet__number\">1.9<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> hjust = <span class=\"code-snippet__number\">0.5<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> angle = <span class=\"code-snippet__number\">90<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.title = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.text = element_text(color=<span class=\"code-snippet__string\">\"black\"<\/span>, # \u8bbe\u7f6e\u56fe\u4f8b\u6807\u7b7e\u6587\u5b57<\/span><\/code><code><span class=\"code-snippet_outer\"> size = <span class=\"code-snippet__number\">10<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> face = <span class=\"code-snippet__string\">\"bold\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text.x = element_text(size = <span class=\"code-snippet__number\">13<\/span>, # \u4fee\u6539X\u8f74\u4e0a\u5b57\u4f53\u5927\u5c0f\uff0c<\/span><\/code><code><span class=\"code-snippet_outer\"> color = <span class=\"code-snippet__string\">\"black\"<\/span>, # \u989c\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"> face = <span class=\"code-snippet__string\">\"bold\"<\/span>, <span class=\"code-snippet__meta\"># face\u53d6\u503c\uff1aplain\u666e\u901a\uff0cbold\u52a0\u7c97\uff0citalic\u659c\u4f53\uff0cbold.italic\u659c\u4f53\u52a0\u7c97<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> vjust = <span class=\"code-snippet__number\">0.5<\/span>, # \u4f4d\u7f6e<\/span><\/code><code><span class=\"code-snippet_outer\"> hjust = <span class=\"code-snippet__number\">0.5<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> angle = <span class=\"code-snippet__number\">0<\/span>), #\u89d2\u5ea6<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text.y = element_text(size = <span class=\"code-snippet__number\">13<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> color = <span class=\"code-snippet__string\">\"black\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> face = <span class=\"code-snippet__string\">\"bold\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> vjust = <span class=\"code-snippet__number\">0.5<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> hjust = <span class=\"code-snippet__number\">0.5<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> angle = <span class=\"code-snippet__number\">0<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\"> )<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u4e0a\u83dc<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__function\">p<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">pdf<\/span><span class=\"code-snippet__params\">(file=<span class=\"code-snippet__string\">\"volcano_plot.pdf\"<\/span>, width=<span class=\"code-snippet__number\">11<\/span>, height=<span class=\"code-snippet__number\">9<\/span>)<\/span>#\u5bfc\u51fa\u56fe\u7247<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">print<\/span><span class=\"code-snippet__params\">(p)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dev.<span class=\"code-snippet__title\">off<\/span><span class=\"code-snippet__params\">()<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8962264150943396\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_4gbBqhpfCQj6ZpRsFoBxdkbn0xJb6Q-1.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QvJwJT1YzjHzVO1g6SoONncj0PNsft0ecRgFtZx2oiaU2tF44gbBqhpfCQj6ZpRsFoBxdkbn0xJb6Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"424\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u8bf7\u4eab\u7528<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u706b\u5c71\u56fe\u7684\u672c\u8d28\u5c31\u662f\u4e00\u5f20\u6563\u70b9\u56fe\uff0c\u6211\u4eec\u53ea\u8981\u7406\u6e05\u4e86\u7ed8\u56fe\u903b\u8f91\uff0c\u65e0\u8bba\u7528\u516c\u5171\u5e73\u53f0\uff08\u4e91\u751f\u4fe1\u751f\u7269\u4fe1\u606f\u5b66\u5e73\u53f0\uff1ahttp:\/\/www.biocloudservice.com\/home.html\uff09\u8fd8\u662f\u548c\u5c0f\u56fe\u5b66\uff08\u516c\u4f17\u53f7\uff1a\u751f\u4fe1\u56fe\uff09\uff0c\u90fd\u53ef\u4ee5\u7ed8\u5236\u51fa\u6f02\u4eae\u7684\u706b\u5c71\u56fe\uff01\u8be5\u7a0b\u5e8f\u8fd8\u8fdc\u8fdc\u4e0d\u6b62\u8fd9\u4e9b\uff0c\u4f8b\u5982\u6587\u5b57\u91cd\u53e0\u3001\u8fde\u7ebf\u4ea4\u53c9\u7b49\u7b49\u3002\u5c0f\u4f19\u4f34\u8d76\u7d27\u52a8\u624b\u505a\u8d77\u6765\uff0c\u76f8\u4fe1\u80af\u5b9a\u7ed8\u5236\u7684\u56fe\u7247\u6bd4\u5c0f\u56fe\u7cbe\u7f8e\u3002<br \/><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u671f\u5f85\u5c0f\u56fe\u751f\u4fe1\u9910\u9986\u4e0b\u6b21\u65b0\u54c1\uff01<\/p>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;border-style: solid;border-width: 1px;border-color: rgb(176, 176, 176);padding: 7px;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgba(249, 238, 237, 0.5);padding: 18px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;font-size: 14px;\">\n<p style=\"white-space: normal;\">\u5230\u6b64\uff0cGEO\u6570\u636e\u5e93\u82af\u7247\u6570\u636e\u7684\u4e0b\u8f7d\uff0cprobe id\u7684\u8f6c\u6362\u5df2\u7ecf\u57fa\u672c\u5b8c\u6210\u4e86\uff0c\u6574\u4e2a\u5206\u6790\u4e2d\u7684\u6570\u636e\u5904\u7406\u5185\u5bb9\u4e5f\u5df2\u7ecf\u57fa\u672c\u89e3\u51b3\u3002\u63a5\u4e0b\u6765\uff0c\u5c31\u662f\u9488\u5bf9\u8fd9\u4e9b\u5dee\u5f02\u57fa\u56e0\u7684\u5e38\u89c4\u5206\u6790\uff0c\u4ee5\u53caGO\u5206\u6790\uff0cKEGG\u5206\u6790\uff0cGSEA\u5206\u6790\uff0c\u86cb\u767d-\u86cb\u767d\u4e92\u4f5c\u7f51\u7edc\uff08Protein-protein interaction\uff0cPPI\uff09\u3002\u8bf7\u671f\u5f85\u5c0f\u56fe\u7684\u4e0b\u671f\u5206\u4eab\uff01\u5982\u6709\u5206\u6790\u95ee\u9898\u6216\u9700\u6c42\u6b22\u8fce\u5c0f\u4f19\u4f34\u4eec\u968f\u65f6\u6765\u548c\u5c0f\u56fe\u63a2\u8ba8\u3002<\/p>\n<p style=\"white-space: normal;\">\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1\u5e73\u53f0 ( http:\/\/www.biocloudservice.com\/home.html)<\/p>\n<\/section>\n<\/section>\n<section style=\"transform: scale(0.5);transform-origin: center center;margin-top: -65px;margin-bottom: -65px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_avcU8Pta8ZVDUicicbRMrPPMJCXCLA-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/2wJ7bWfS0QvJwJT1YzjHzVO1g6SoONncibXefNicnUH8pklkibRqLicnTa2NGPiavcU8Pta8ZVDUicicbRMrPPMJCXCLA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"258\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"min-height: 40px;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"width: 100%;margin-right: auto;margin-bottom: -10px;margin-left: auto;\">\n<table width=\"100%\">\n<tbody>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:29.classicTable1:0\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:29.classicTable1:0.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;background-color: rgb(249, 174, 165);padding: 10px 0px;\" width=\"96.3700%\">\n<section style=\"line-height: 1;text-align: center;font-size: 17px;color: rgb(255, 255, 255);letter-spacing: 5px;\" powered-by=\"xiumi.us\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:29.classicTable1:1\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:29.classicTable1:1.td@@0\" style=\"border-top: 0px none rgb(62, 62, 62);border-right: 0px none rgb(62, 62, 62);border-left: 0px none rgb(62, 62, 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href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkxODUxNDgzNA==&mid=2247483730&idx=1&sn=abc7095012e15da0a0c27ec4d79ec243&chksm=c1b17caff6c6f5b93c5fc3b02da32d7e65aaf6063f50cde671373bf81595115c30ae0235ffb1&scene=21#wechat_redirect\" textvalue=\"GSEA\u5bcc\u96c6\u5206\u6790\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">GSEA\u5bcc\u96c6\u5206\u6790<\/a><\/p>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:29.classicTable1:3\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:29.classicTable1:3.td@@0\" style=\"border-top: 0px none rgb(62, 62, 62);border-right: 0px none rgb(62, 62, 62);border-left: 0px none rgb(62, 62, 62);border-bottom-color: rgb(202, 198, 198);border-bottom-style: dashed;background-color: rgb(247, 247, 247);padding-right: 5px;padding-left: 5px;\" width=\"96.3700%\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: middle;border-width: 0px;align-self: center;flex: 0 0 auto;\">\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkxODUxNDgzNA==&mid=2247483716&idx=1&sn=13be92cf86d7f856532fc75783a70115&chksm=c1b17cb9f6c6f5afb518568114f874db73df789d3ffd7a7c2c45192748a3aede5441bf598669&scene=21#wechat_redirect\" textvalue=\"\u4e00\u6587\u89e3\u51b3GEO\u82af\u7247\u6570\u636e\u63a2\u9488ID\u8f6c\u6362\u7684\u5de5\u4f5c\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u4e00\u6587\u89e3\u51b3GEO\u82af\u7247\u6570\u636e\u63a2\u9488ID\u8f6c\u6362\u7684\u5de5\u4f5c<\/a><\/p>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/section>\n<section style=\"width: 100%;margin-right: auto;margin-bottom: -10px;margin-left: auto;\"><span style=\"outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;background-color: rgb(255, 255, 255);letter-spacing: 0.578px;\">\ud83d\udc47<\/span><span style=\"font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 0.544px;background-color: rgb(255, 255, 255);\">\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8fdb\u5165\u7f51\u5740<\/span><\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div 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[…]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58112"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=58112"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58112\/revisions"}],"predecessor-version":[{"id":58119,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58112\/revisions\/58119"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=58112"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=58112"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=58112"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

{"id":58112,"date":"2024-06-16T22:25:35","date_gmt":"2024-06-16T14:25:35","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=58112"},"modified":"2024-06-16T22:25:35","modified_gmt":"2024-06-16T14:25:35","slug":"volcano-plot-%e5%88%ab%e5%86%8d%e9%97%ae%e6%88%91%e8%bf%99%e4%b8%ba%e4%bb%80%e4%b9%88%e6%98%af%e7%81%ab%e5%b1%b1%e5%9b%be-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=58112","title":{"rendered":"Volcano plot\u2014\u2014\u522b\u518d\u95ee\u6211\u8fd9\u4e3a\u4ec0\u4e48\u662f\u706b\u5c71\u56fe"},"content":{"rendered":"

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data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"Volcano plot\u2014\u2014\u522b\u518d\u95ee\u6211\u8fd9\u4e3a\u4ec0\u4e48\u662f\u706b\u5c71\u56fe\",\"time\":\"2023-07-13 19:00:16\",\"timeStamp\":\"1689246016\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247490042&idx=3&sn=3fe6c12e4097dc6e4e91cf53458153a2&chksm=cf7c893ff80b0029343c53f081b504a4b5708b6c1b3f82723d28df751247a49e19e69a3f6c73#rd\" target=\"_blank\" rel=\"noopener\">Volcano plot\u2014\u2014\u522b\u518d\u95ee\u6211\u8fd9\u4e3a\u4ec0\u4e48\u662f\u706b\u5c71\u56fe<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u56fe <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" 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style=\"font-size: 17px;color: rgb(255, 255, 255);text-align: right;letter-spacing: 4px;\" powered-by=\"xiumi.us\">\n<p><strong>\u70b9\u51fb\u84dd\u5b57<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;border-left: 4px solid rgb(242, 183, 183);border-bottom-left-radius: 0px;flex: 100 100 0%;align-self: stretch;height: auto;padding: 3px 10px;background-color: rgba(254, 245, 240, 0.8);\">\n<section style=\"color: rgb(181, 126, 121);font-size: 17px;letter-spacing: 4px;\" powered-by=\"xiumi.us\">\n<p><strong>\u5173\u6ce8\u5c0f\u56fe<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">\u4eca\u5929\u5c0f\u56fe\u751f\u4fe1\u9910\u9986\u7ed9\u5c0f\u4f19\u4f34\u63a8\u8350\u9053\u751f\u4fe1\u9910\uff0c\u80fd\u91cf\u8f7b\u9910\uff1a\u201c\u706b\u5c71\u5305\u201d<\/p>\n<p style=\"text-indent: 2em;white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u80fd\u91cf\u6e90\u4e8e\u771f\u6750\u5b9e\u6599<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">\u706b\u5c71\u56fe\uff08Volcano plot\uff09\u662f\u4e00\u79cd\u6bd4\u8f83\u201c\u8fdc\u53e4\u201d\u7684\u4e00\u79cd\u6563\u70b9\u56fe\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u57fa\u56e0\u7ec4\u3001\u8f6c\u5f55\u7ec4\u3001\u86cb\u767d\u7ec4\u7b49\u7ec4\u95f4\u5dee\u5f02\u5206\u6790\u7ed3\u679c\u7684\u5c55\u793a\u3002\u56fe\u8868\u7684\u6a2a\u8f74\u4e00\u822c\u5c55\u793a\u5dee\u5f02\u500d\u6570\u7684\u53d8\u5316\uff0c\u800c\u7eb5\u8f74\u8868\u793a\u5dee\u5f02\u5206\u6790\u7ed3\u679c\u7684\u53ef\u9760\u6027\u3002\u901a\u8fc7\u627e\u5230\u663e\u8457\u5dee\u5f02\u7684\u57fa\u56e0\uff0c\u7814\u7a76\u8005\u53ef\u4ee5\u8fdb\u4e00\u6b65\u505a\u540e\u7eed\u7684\u5b9e\u9a8c\u3002<\/p>\n<p style=\"text-indent: 2em;white-space: normal;\"><br \/><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u5728\u751f\u7269\u4fe1\u606f\u5206\u6790\u4e2d\uff0c\u706b\u5c71\u56fe\u662f\u975e\u5e38\u5e38\u89c1\u7684\u4e00\u79cd\u6570\u636e\u5c55\u793a\u5f62\u5f0f\u3002\u7531\u4e8e\u706b\u5c71\u56fe\u53ef\u4ee5\u975e\u5e38\u6e05\u6670\u7684\u5c55\u793a\u51fa\u54ea\u4e9b\u57fa\u56e0\u5728\u4e0d\u540c\u6837\u672c\u4e2d\u662f\u5177\u6709\u5dee\u5f02\u8868\u8fbe\u663e\u8457\u6027\u7684\u57fa\u56e0\uff0c\u56e0\u6b64\u5728\u751f\u7269\u533b\u5b66\u4e2d\uff0c\u5e38\u5e94\u7528\u4e8e\u75c5\u4f8b\u548c\u5bf9\u7167\u7ec4\u7684\u8f6c\u5f55\u7ec4\u7814\u7a76\uff0c\u4e5f\u80fd\u5e94\u7528\u4e8e\u57fa\u56e0\u7ec4\uff0c\u86cb\u767d\u8d28\u7ec4\uff0c\u4ee3\u8c22\u7ec4\u7b49\u7edf\u8ba1\u6570\u636e\u3002<\/p>\n<p style=\"text-indent: 2em;white-space: normal;\"><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u8f7b\u98df\u83dc\u5355<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\">\u5e38\u89c1\u706b\u5c71\u56fe\u7c7b\u578b\uff1a<\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.002409638554217\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_JqfnBibcQaOW1767zReRL1icsSPdqA-1.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QvJwJT1YzjHzVO1g6SoONncKW7bHZLO6cvLMDc1ibw7KX5mlzCJqfnBibcQaOW1767zReRL1icsSPdqA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"415\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.0192771084337349\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_K9IxYgKpY8VK1ribyvD0Z7SJMicBAg-1.png?w=640\" 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data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<ul style=\"list-style-type: \uf06c;padding-left: 40px;list-style-position: outside;\" class=\"list-paddingleft-1\" powered-by=\"xiumi.us\">\n<li>\n<p>\u6bcf\u4e2a\u70b9\u4ee3\u8868\u4e00\u4e2a\u68c0\u6d4b\u5230\u7684\u57fa\u56e0\u3002<\/p>\n<\/li>\n<li>\n<p>\u6a2a\u8f74\u548c\u7eb5\u8f74\u7528\u4e8e\u56fa\u5b9a\u70b9\u5728\u7a7a\u95f4\u7684\u4f4d\u7f6e\u3002<\/p>\n<\/li>\n<li>\n<p>\u4e00\u822c\u6a2a\u8f74\u662fLog2(fold change)\uff0c\u70b9\u8d8a\u504f\u79bb\u4e2d\u5fc3\uff0c\u8868\u793a\u5dee\u5f02\u500d\u6570\u8d8a\u5927\u3002<\/p>\n<\/li>\n<li>\n<p>\u7eb5\u8f74\u662f-Log 10 (pvalue)\uff0c\u70b9\u8d8a\u9760\u56fe\u7684\u9876\u90e8\u8868\u793a\u5dee\u5f02\u8d8a\u663e\u8457\u3002<\/p>\n<\/li>\n<li>\n<p>\u70b9\u7684\u5927\u5c0f\u548c\u989c\u8272\u4e5f\u53ef\u4ee5\u8868\u793a\u66f4\u591a\u7684\u5c5e\u6027\uff0c\u5982\u540e\u9762\u56fe\u4e2d\u70b9\u7684\u989c\u8272\u6807\u8bb0\u5176\u5bf9\u5e94\u7684\u57fa\u56e0\u662f\u4e0a\u8c03, \u4e0b\u8c03\u8fd8\u662f\u65e0\u5dee\u5f02\u3002<\/p>\n<\/li>\n<li>\n<p>\u5927\u5c0f\u4e5f\u53ef\u7528\u4e8e\u5c55\u793a\u57fa\u56e0\u8868\u8fbe\u7684\u5e73\u5747\u4e30\u5ea6\uff0c\u4e00\u822c\u6211\u4eec\u5173\u6ce8\u8868\u8fbe\u6c34\u5e73\u8f83\u9ad8\u4e14\u5dee\u5f02\u8f83\u5927\u7684\u57fa\u56e0\u7528\u4e8e\u540e\u7eed\u7684\u5206\u6790\u548c\u9a8c\u8bc1\u3002<\/p>\n<\/li>\n<\/ul>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u706b\u5c71\u56fe\u662f\u8bb8\u591a\u751f\u4fe1\u5e73\u53f0\u9996\u6279\u4e0a\u7ebf\u7684\u5e38\u89c1\u56fe\uff0c\u867d\u7136\u6211\u4eec\u7ecf\u8fc7\u4e86\u5927\u91cf\u4f18\u5316\uff0c\u589e\u52a0\u4e86\u8bf8\u591a\u53ef\u8c03\u53c2\u6570\uff1a\u989c\u8272\uff0c\u70b9\u5927\u5c0f\uff0c\u751a\u81f3legend\u7684\u4f4d\u7f6e\u548c\u987a\u5e8f\u90fd\u8fdb\u884c\u4e86\u4f18\u5316\uff0c\u4f46\u662f\uff0c\u8fd8\u662f\u4e0d\u80fd\u6ee1\u8db3\u5c0f\u4f19\u4f34\u7684\u9700\u6c42\uff1a<\/p>\n<p style=\"white-space: normal;\">\u80fd\u4e0d\u80fd\u6807\u6ce8\u6211\u611f\u5174\u8da3\u7684\u57fa\u56e0\uff1f<\/p>\n<p style=\"white-space: normal;\">\u80fd\u4e0d\u80fd\u7ed9\u70b9\u52a0\u4e0a\u900f\u660e\u5ea6\uff1f<\/p>\n<p style=\"white-space: normal;\">\u80fd\u4e0d\u80fd\u989d\u5916\u52a0\u4e9b\u7ebf\uff1f<\/p>\n<p style=\"white-space: normal;\">\u80fd\u4e0d\u80fd\u653e\u5927\u67d0\u4e9b\u70b9\uff1f<\/p>\n<p style=\"white-space: normal;\">\u80fd\u4e0d\u80fd\u628ap\u503c\u4e3a0\u7684\u70b9\u9608\u503c\u5bbd\u677e\u70b9\uff1f<\/p>\n<p style=\"white-space: normal;\">\u80fd\u4e0d\u80fd\u6807\u6ce8\u4e0b\u4e0a\u8c03\u591a\u5c11\uff0c\u4e0b\u8c03\u591a\u5c11\uff1f<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">\u5c0f\u56fe\u53ef\u4ee5\u8d1f\u8d23\u4efb\u5730\u544a\u8bc9\u5c0f\u4f19\u4f34\uff0c\u90fd\u80fd\u6ee1\u8db3\uff0cBut\uff0c\u4efb\u4f55\u5728\u7ebf\u5de5\u5177\u90fd\u4e0d\u80fd\u6ee1\u8db3\u60a8\u7684\u5b9a\u5236\u9700\u6c42\u3002\u8981\u60f3\u6ee1\u8db3\uff0c\u9700\u8981\u4f60\u81ea\u5df1\u7f16\u7a0b\u6216\u8005\u7f16\u8f91\u751f\u6210\u7684\u56fe\u3002<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u4eca\u5929\uff0c\u7ed9\u5c0f\u4f19\u4f34\u5e26\u6765\u7684\u5236\u4f5c\u6750\u6599\u662fRColorBrewer\u3001ggrepel\u3001ggplot R\u5305\u3002\u8fd9\u91cc\u5c0f\u56fe\u7ed9\u4e0d\u61c2\u7f16\u7a0b\u7684\u5c0f\u4f19\u4f34\u5e26\u6765\u4e0d\u4e00\u6837\u7684\u706b\u5c71\u56fe\u4f53\u9a8c\u3002\u8d70\u8d77\u2014\u2014\u2014\u2014<\/p>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u5236\u4f5c\u8fc7\u7a0b<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">rm(<span class=\"code-snippet__built_in\">list<\/span> = ls())#\u6e05\u9664\u6240\u6709\u7684\u53d8\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">library(RColorBrewer)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggrepel) #\u6807\u7b7e\u7528<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u63a5\u4e0b\u6765\u51c6\u5907\u4f5c\u56fe\u7684\u6570\u636e<br \/><\/p>\n<p style=\"white-space: normal;\">\u4eca\u5929\u7528\u5230\u7684geo\u6570\u636e\u662f\u7ecflimma\u5dee\u5f02\u5206\u6790\u540e\u7684\uff0c\u6570\u636e\u7684\u683c\u5f0f\u662f\u6587\u672ctext\uff0c\u53ef\u76f4\u63a5\u5bfc\u5165\u5230R\u4e2d\uff1a<\/p>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6105263157894737\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_tMma2iaLsf7OAZsuz6qNbuFTQibCHg-1.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QvJwJT1YzjHzVO1g6SoONnchdMVbYnZmHXYzcpiaf0oLrdSibibptMma2iaLsf7OAZsuz6qNbuFTQibCHg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"380\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">'D:\\limma'<\/span>)#\u52a0\u8f7d\u8def\u5f84<\/span><\/code><code><span class=\"code-snippet_outer\">df=read.table(<span class=\"code-snippet__string\">\"\u706b\u5c71\u56fe\u8f93\u5165\u6570\u636e.txt\"<\/span>,sep=<span class=\"code-snippet__string\">'t'<\/span>,header = T)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u786e\u5b9a\u662f\u4e0a\u8c03\u8fd8\u662f\u4e0b\u8c03\uff0c\u7528\u4e8e\u7ed9\u56fe\u4e2d\u70b9\u4e0a\u8272<\/span><\/code><code><span class=\"code-snippet_outer\">df$threshold = factor(ifelse(df$pvalue < <span class=\"code-snippet__number\">0.05<\/span> & <span class=\"code-snippet__built_in\">abs<\/span>(df$log2FoldChange) >= <span class=\"code-snippet__number\">0.25<\/span>, ifelse(df$log2FoldChange >= <span class=\"code-snippet__number\">0.25<\/span> ,<span class=\"code-snippet__string\">'Up'<\/span>,<span class=\"code-snippet__string\">'Down'<\/span>),<span class=\"code-snippet__string\">'NoSignifi'<\/span>),levels=c(<span class=\"code-snippet__string\">'Up'<\/span>,<span class=\"code-snippet__string\">'Down'<\/span>,<span class=\"code-snippet__string\">'NoSignifi'<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\">df$gene <- row.names(df) #\u6dfb\u52a0\u4e00\u5217\u57fa\u56e0\u540d\uff0c\u4ee5\u4fbf\u5907\u6ce8<\/span><\/code><code><span class=\"code-snippet_outer\">p <- ggplot(df,aes(x=log2FoldChange,y= -<span class=\"code-snippet__built_in\">log10<\/span>(pvalue),color=threshold))+<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(data = df[df$pvalue<<span class=\"code-snippet__number\">0.05<\/span>&<span class=\"code-snippet__built_in\">abs<\/span>(df$log2FoldChange)><span class=\"code-snippet__number\">0.25<\/span>,],size = <span class=\"code-snippet__number\">0.5<\/span>)+ <\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(data = df[df$pvalue><span class=\"code-snippet__number\">0.05<\/span>|<span class=\"code-snippet__built_in\">abs<\/span>(df$log2FoldChange)<<span class=\"code-snippet__number\">0.25<\/span>,],size = <span class=\"code-snippet__number\">0.5<\/span>)+<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_color_manual(values=c(<span class=\"code-snippet__string\">\"#4393C3\"<\/span>,<span class=\"code-snippet__string\">\"#00000033\"<\/span>,<span class=\"code-snippet__string\">\"#FC4E2A\"<\/span>))+#\u786e\u5b9a\u70b9\u7684\u989c\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_text_repel(<\/span><\/code><code><span class=\"code-snippet_outer\"> data = df[df$pvalue<<span class=\"code-snippet__number\">0.05<\/span>&<span class=\"code-snippet__built_in\">abs<\/span>(df$fd)><span class=\"code-snippet__number\">1<\/span>,],<\/span><\/code><code><span class=\"code-snippet_outer\"> aes(label = gene),<\/span><\/code><code><span class=\"code-snippet_outer\"> size = <span class=\"code-snippet__number\">4.5<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> color = <span class=\"code-snippet__string\">\"black\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> segment.color = <span class=\"code-snippet__string\">\"black\"<\/span>, show.legend = FALSE )+#\u6dfb\u52a0\u5173\u6ce8\u7684\u70b9\u7684\u57fa\u56e0\u540d<\/span><\/code><code><span class=\"code-snippet_outer\"> ylab(<span class=\"code-snippet__string\">'-log10 (P_value)'<\/span>)+#\u4fee\u6539y\u8f74\u540d\u79f0<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab(<span class=\"code-snippet__string\">'log2 (FoldChange)'<\/span>)+#\u4fee\u6539x\u8f74\u540d\u79f0<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_vline(xintercept=c(<span class=\"code-snippet__number\">-0.25<\/span>,<span class=\"code-snippet__number\">0.25<\/span>),lty=<span class=\"code-snippet__number\">3<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.5<\/span>) +#\u6dfb\u52a0\u6a2a\u7ebf|logFoldChange|><span class=\"code-snippet__number\">0.25<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> geom_hline(yintercept = -<span class=\"code-snippet__built_in\">log10<\/span>(<span class=\"code-snippet__number\">0.05<\/span>),lty=<span class=\"code-snippet__number\">3<\/span>,col=<span class=\"code-snippet__string\">\"black\"<\/span>,lwd=<span class=\"code-snippet__number\">0.5<\/span>) +#\u6dfb\u52a0\u7ad6\u7ebfpadj<<span class=\"code-snippet__number\">0.05<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> theme_classic( # \u4e3b\u9898\u8bbe\u7f6e\uff0c\u8fd9\u4e2a\u662f\u65e0\u7ebf\u6761\u4e3b\u9898<\/span><\/code><code><span class=\"code-snippet_outer\"> base_line_size = <span class=\"code-snippet__number\">1<\/span> # \u5750\u6807\u8f74\u7684\u7c97\u7ec6<\/span><\/code><code><span class=\"code-snippet_outer\"> )+<\/span><\/code><code><span class=\"code-snippet_outer\"> theme(axis.title.x = element_text(size = <span class=\"code-snippet__number\">15<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> color = <span class=\"code-snippet__string\">\"black\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> face = <span class=\"code-snippet__string\">\"bold\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.title.y = element_text(size = <span class=\"code-snippet__number\">15<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> color = <span class=\"code-snippet__string\">\"black\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> face = <span class=\"code-snippet__string\">\"bold\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> vjust = <span class=\"code-snippet__number\">1.9<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> hjust = <span class=\"code-snippet__number\">0.5<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> angle = <span class=\"code-snippet__number\">90<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.title = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> legend.text = element_text(color=<span class=\"code-snippet__string\">\"black\"<\/span>, # \u8bbe\u7f6e\u56fe\u4f8b\u6807\u7b7e\u6587\u5b57<\/span><\/code><code><span class=\"code-snippet_outer\"> size = <span class=\"code-snippet__number\">10<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> face = <span class=\"code-snippet__string\">\"bold\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text.x = element_text(size = <span class=\"code-snippet__number\">13<\/span>, # \u4fee\u6539X\u8f74\u4e0a\u5b57\u4f53\u5927\u5c0f\uff0c<\/span><\/code><code><span class=\"code-snippet_outer\"> color = <span class=\"code-snippet__string\">\"black\"<\/span>, # \u989c\u8272<\/span><\/code><code><span class=\"code-snippet_outer\"> face = <span class=\"code-snippet__string\">\"bold\"<\/span>, <span class=\"code-snippet__meta\"># face\u53d6\u503c\uff1aplain\u666e\u901a\uff0cbold\u52a0\u7c97\uff0citalic\u659c\u4f53\uff0cbold.italic\u659c\u4f53\u52a0\u7c97<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> vjust = <span class=\"code-snippet__number\">0.5<\/span>, # \u4f4d\u7f6e<\/span><\/code><code><span class=\"code-snippet_outer\"> hjust = <span class=\"code-snippet__number\">0.5<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> angle = <span class=\"code-snippet__number\">0<\/span>), #\u89d2\u5ea6<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text.y = element_text(size = <span class=\"code-snippet__number\">13<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> color = <span class=\"code-snippet__string\">\"black\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> face = <span class=\"code-snippet__string\">\"bold\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> vjust = <span class=\"code-snippet__number\">0.5<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> hjust = <span class=\"code-snippet__number\">0.5<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> angle = <span class=\"code-snippet__number\">0<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\"> )<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u4e0a\u83dc<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__function\">p<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">pdf<\/span><span class=\"code-snippet__params\">(file=<span class=\"code-snippet__string\">\"volcano_plot.pdf\"<\/span>, width=<span class=\"code-snippet__number\">11<\/span>, height=<span class=\"code-snippet__number\">9<\/span>)<\/span>#\u5bfc\u51fa\u56fe\u7247<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">print<\/span><span class=\"code-snippet__params\">(p)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">dev.<span class=\"code-snippet__title\">off<\/span><span class=\"code-snippet__params\">()<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.8962264150943396\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_4gbBqhpfCQj6ZpRsFoBxdkbn0xJb6Q-1.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/2wJ7bWfS0QvJwJT1YzjHzVO1g6SoONncj0PNsft0ecRgFtZx2oiaU2tF44gbBqhpfCQj6ZpRsFoBxdkbn0xJb6Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"424\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"margin: 10px 0%;text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;border-left: 5px solid rgb(242, 203, 203);border-bottom-left-radius: 0px;padding-left: 9px;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-start;\">\n<section style=\"margin: 2px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(110, 75, 36);font-size: 17px;line-height: 1.3;text-align: justify;letter-spacing: 1px;\">\n<p style=\"white-space: normal;\"><strong>\u8bf7\u4eab\u7528<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u706b\u5c71\u56fe\u7684\u672c\u8d28\u5c31\u662f\u4e00\u5f20\u6563\u70b9\u56fe\uff0c\u6211\u4eec\u53ea\u8981\u7406\u6e05\u4e86\u7ed8\u56fe\u903b\u8f91\uff0c\u65e0\u8bba\u7528\u516c\u5171\u5e73\u53f0\uff08\u4e91\u751f\u4fe1\u751f\u7269\u4fe1\u606f\u5b66\u5e73\u53f0\uff1ahttp:\/\/www.biocloudservice.com\/home.html\uff09\u8fd8\u662f\u548c\u5c0f\u56fe\u5b66\uff08\u516c\u4f17\u53f7\uff1a\u751f\u4fe1\u56fe\uff09\uff0c\u90fd\u53ef\u4ee5\u7ed8\u5236\u51fa\u6f02\u4eae\u7684\u706b\u5c71\u56fe\uff01\u8be5\u7a0b\u5e8f\u8fd8\u8fdc\u8fdc\u4e0d\u6b62\u8fd9\u4e9b\uff0c\u4f8b\u5982\u6587\u5b57\u91cd\u53e0\u3001\u8fde\u7ebf\u4ea4\u53c9\u7b49\u7b49\u3002\u5c0f\u4f19\u4f34\u8d76\u7d27\u52a8\u624b\u505a\u8d77\u6765\uff0c\u76f8\u4fe1\u80af\u5b9a\u7ed8\u5236\u7684\u56fe\u7247\u6bd4\u5c0f\u56fe\u7cbe\u7f8e\u3002<br \/><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">\u671f\u5f85\u5c0f\u56fe\u751f\u4fe1\u9910\u9986\u4e0b\u6b21\u65b0\u54c1\uff01<\/p>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;border-style: solid;border-width: 1px;border-color: rgb(176, 176, 176);padding: 7px;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgba(249, 238, 237, 0.5);padding: 18px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;font-size: 14px;\">\n<p style=\"white-space: normal;\">\u5230\u6b64\uff0cGEO\u6570\u636e\u5e93\u82af\u7247\u6570\u636e\u7684\u4e0b\u8f7d\uff0cprobe id\u7684\u8f6c\u6362\u5df2\u7ecf\u57fa\u672c\u5b8c\u6210\u4e86\uff0c\u6574\u4e2a\u5206\u6790\u4e2d\u7684\u6570\u636e\u5904\u7406\u5185\u5bb9\u4e5f\u5df2\u7ecf\u57fa\u672c\u89e3\u51b3\u3002\u63a5\u4e0b\u6765\uff0c\u5c31\u662f\u9488\u5bf9\u8fd9\u4e9b\u5dee\u5f02\u57fa\u56e0\u7684\u5e38\u89c4\u5206\u6790\uff0c\u4ee5\u53caGO\u5206\u6790\uff0cKEGG\u5206\u6790\uff0cGSEA\u5206\u6790\uff0c\u86cb\u767d-\u86cb\u767d\u4e92\u4f5c\u7f51\u7edc\uff08Protein-protein interaction\uff0cPPI\uff09\u3002\u8bf7\u671f\u5f85\u5c0f\u56fe\u7684\u4e0b\u671f\u5206\u4eab\uff01\u5982\u6709\u5206\u6790\u95ee\u9898\u6216\u9700\u6c42\u6b22\u8fce\u5c0f\u4f19\u4f34\u4eec\u968f\u65f6\u6765\u548c\u5c0f\u56fe\u63a2\u8ba8\u3002<\/p>\n<p style=\"white-space: normal;\">\u6b22\u8fce\u4f7f\u7528\uff1a\u4e91\u751f\u4fe1\u5e73\u53f0 ( http:\/\/www.biocloudservice.com\/home.html)<\/p>\n<\/section>\n<\/section>\n<section style=\"transform: scale(0.5);transform-origin: center center;margin-top: -65px;margin-bottom: -65px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_avcU8Pta8ZVDUicicbRMrPPMJCXCLA-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/2wJ7bWfS0QvJwJT1YzjHzVO1g6SoONncibXefNicnUH8pklkibRqLicnTa2NGPiavcU8Pta8ZVDUicicbRMrPPMJCXCLA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"258\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"min-height: 40px;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"width: 100%;margin-right: auto;margin-bottom: -10px;margin-left: auto;\">\n<table width=\"100%\">\n<tbody>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:29.classicTable1:0\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:29.classicTable1:0.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;background-color: rgb(249, 174, 165);padding: 10px 0px;\" width=\"96.3700%\">\n<section style=\"line-height: 1;text-align: center;font-size: 17px;color: rgb(255, 255, 255);letter-spacing: 5px;\" powered-by=\"xiumi.us\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:29.classicTable1:1\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:29.classicTable1:1.td@@0\" style=\"border-top: 0px none rgb(62, 62, 62);border-right: 0px none rgb(62, 62, 62);border-left: 0px none rgb(62, 62, 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rel=\"noopener\">\u6700\u597d\u61c2\u7684GEO\u6570\u636e\u4e0b\u8f7d\u548c\u9884\u5904\u7406\uff0c\u4f60\u8981\u770b\u5417\uff1f<\/a><\/p>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:29.classicTable1:2\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:29.classicTable1:2.td@@0\" style=\"border-top: 0px none rgb(62, 62, 62);border-right: 0px none rgb(62, 62, 62);border-left: 0px none rgb(62, 62, 62);border-bottom-color: rgb(202, 198, 198);border-bottom-style: dashed;background-color: rgb(247, 247, 247);padding-right: 5px;padding-left: 5px;\" width=\"96.3700%\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: middle;border-width: 0px;align-self: center;flex: 0 0 auto;\">\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" 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center;justify-content: center;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: middle;border-width: 0px;align-self: center;flex: 0 0 auto;\">\n<p powered-by=\"xiumi.us\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkxODUxNDgzNA==&mid=2247483716&idx=1&sn=13be92cf86d7f856532fc75783a70115&chksm=c1b17cb9f6c6f5afb518568114f874db73df789d3ffd7a7c2c45192748a3aede5441bf598669&scene=21#wechat_redirect\" textvalue=\"\u4e00\u6587\u89e3\u51b3GEO\u82af\u7247\u6570\u636e\u63a2\u9488ID\u8f6c\u6362\u7684\u5de5\u4f5c\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u4e00\u6587\u89e3\u51b3GEO\u82af\u7247\u6570\u636e\u63a2\u9488ID\u8f6c\u6362\u7684\u5de5\u4f5c<\/a><\/p>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/section>\n<section style=\"width: 100%;margin-right: auto;margin-bottom: -10px;margin-left: auto;\"><span style=\"outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;background-color: rgb(255, 255, 255);letter-spacing: 0.578px;\">\ud83d\udc47<\/span><span style=\"font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 0.544px;background-color: rgb(255, 255, 255);\">\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8fdb\u5165\u7f51\u5740<\/span><\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div 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[…]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58112"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=58112"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58112\/revisions"}],"predecessor-version":[{"id":58119,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58112\/revisions\/58119"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=58112"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=58112"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=58112"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}