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style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(246, 248, 247);padding: 25px;\">\n<section style=\"text-align: justify;letter-spacing: 0.5px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 2.0625em;margin-bottom: 12px;text-wrap: 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onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-05_\u300c\u8f6c\u300d\u7b80\u5355\u7c97\u66b4\uff01\u4f4e\u65b9\u5dee\u6ee4\u6ce2VS\u9ad8\u76f8\u5173\u6ee4\u6ce2\uff0c\u54ea\u4e2a\u66f4\u7b80\u5355\uff1f\u4e00\u62db\u8ba9\u4f60\u7684\u6570\u636e\u53d8\u5f97\u8f7b\u677e\u65e0\u6bd4\uff01\uff08\u4e0a\uff09\/4_CiaN7s0q9HZZaRlJ3BoSMR3nbR2Bkw.png\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-05_\u300c\u8f6c\u300d\u7b80\u5355\u7c97\u66b4\uff01\u4f4e\u65b9\u5dee\u6ee4\u6ce2VS\u9ad8\u76f8\u5173\u6ee4\u6ce2\uff0c\u54ea\u4e2a\u66f4\u7b80\u5355\uff1f\u4e00\u62db\u8ba9\u4f60\u7684\u6570\u636e\u53d8\u5f97\u8f7b\u677e\u65e0\u6bd4\uff01\uff08\u4e0a\uff09\/5_CiaN7s0q9HZZaRlJ3BoSMR3nbR2Bkw.png\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-05_\u300c\u8f6c\u300d\u7b80\u5355\u7c97\u66b4\uff01\u4f4e\u65b9\u5dee\u6ee4\u6ce2VS\u9ad8\u76f8\u5173\u6ee4\u6ce2\uff0c\u54ea\u4e2a\u66f4\u7b80\u5355\uff1f\u4e00\u62db\u8ba9\u4f60\u7684\u6570\u636e\u53d8\u5f97\u8f7b\u677e\u65e0\u6bd4\uff01\uff08\u4e0a\uff09\/7_CiaN7s0q9HZZaRlJ3BoSMR3nbR2Bkw.png\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-05_\u300c\u8f6c\u300d\u7b80\u5355\u7c97\u66b4\uff01\u4f4e\u65b9\u5dee\u6ee4\u6ce2VS\u9ad8\u76f8\u5173\u6ee4\u6ce2\uff0c\u54ea\u4e2a\u66f4\u7b80\u5355\uff1f\u4e00\u62db\u8ba9\u4f60\u7684\u6570\u636e\u53d8\u5f97\u8f7b\u677e\u65e0\u6bd4\uff01\uff08\u4e0a\uff09\/8_CiaN7s0q9HZZaRlJ3BoSMR3nbR2Bkw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEWvVqOBicHvFuDE5TR9k3Dk6lH2zXemAzicuLTokHibcUiaOrMNeCiaN7s0q9HZZaRlJ3BoSMR3nbR2Bkw\/640?wx_fmt=png''''''\" data-type=\"png\" data-w=\"500\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 100 100 0%;height: auto;\">\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 7px;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(93, 91, 92);height: 3px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(246, 248, 247);padding: 25px;\">\n<section style=\"text-align: justify;letter-spacing: 0.5px;\" powered-by=\"xiumi.us\">\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">\u4eca\u5929\u5c0f\u5e08\u59b9\u4ee5\u86cb\u767d\u8d28\u8868\u8fbe\u6570\u636e\u4e3a\u4f8b\uff0c\u5e26\u5927\u5bb6\u4e00\u8d77\u8bd5\u8bd5\u5427\uff5e<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">\u9996\u5148\u5bfc\u5165\u6570\u636e\uff0c\u5220\u9664\u7f3a\u5931\u503c<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">library(limma)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">library(edgeR)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">data=read.csv(file.choose(),header = TRUE)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">data <- na.omit(data)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">\u5728\u539f\u59cb\u6570\u636e\u4e2d\u4e0d\u540c\u57fa\u56e0\u5c5e\u4e8e\u4e0d\u540c\u5217\uff0c\u4e3a\u4e86\u65b9\u4fbf\u64cd\u4f5c\uff0c\u9700\u8981\u5bf9\u6570\u636e\u8fdb\u884c\u8f6c\u7f6e<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">X <- data[,2:79]<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"># \u4e3adata\u8bbe\u7f6e\u65b0\u5217<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">X$Genotype <- ifelse(X$Genotype == “Control”, 1, 0)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">Xt <- t(X)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">\u4f7f\u7528filterByExpr\u51fd\u6570\u8fdb\u884c\u4f4e\u65b9\u5dee\u6ee4\u6ce2\uff0c\u53bb\u6389\u8868\u8fbe\u91cf\u5c0f\u4e8e1\u7684\u57fa\u56e0<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">keep <- filterByExpr(Xt,group = Xt[78,], min.count=1)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">X_filtered <- Xt[keep, ]<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">\u67e5\u770b\u6ee4\u6ce2\u524d\u540e\u57fa\u56e0\u6570<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">cat(“Number of genes before filtering:”, nrow(Xt), “n”)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">cat(“Number of genes after filtering:”, nrow(X_filtered), “n”)<\/p>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.12734082397003746\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_aYa9jJpkd4ImzBUAfIiczen2QHpZFQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEWvVqOBicHvFuDE5TR9k3Dk6qN0HSPibGE6Vld4joNnrrOeQLiciaYa9jJpkd4ImzBUAfIiczen2QHpZFQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1068\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(246, 248, 247);padding: 25px;\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 100 100 0%;height: auto;\">\n<section style=\"overflow: hidden;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;\">\n<section style=\"transform: rotate(0.1deg);\">\n<section style=\"width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 100%;margin-right: -6px;margin-left: -7px;border-bottom: 2px dotted rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;padding-right: 32px;padding-left: 32px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;transform: translate3d(11px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(93, 91, 92);min-width: 5%;flex: 0 0 auto;height: auto;align-self: stretch;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;transform: translate3d(-22px, 0px, 0px);margin-top: 4px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"text-align: center;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 42px;height: auto;\"><img decoding=\"async\" data-ratio=\"0.986\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_cE6XdQiasqicVAIAia5LHVEXScnBfw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEWvVqOBicHvFuDE5TR9k3Dk6tGXWuiapfpFrdmg6hiaffco2EAxRricE6XdQiasqicVAIAia5LHVEXScnBfw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"500\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;min-width: 5%;flex: 0 0 auto;height: auto;padding-left: 14px;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(255, 255, 255);\">\n<p><strong>\u6700\u540e<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;height: auto;align-self: center;\">\n<section style=\"overflow: hidden;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;\">\n<section style=\"transform: rotate(0.1deg);\">\n<section style=\"width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 100%;margin-right: -6px;margin-left: -7px;border-bottom: 2px dotted rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;letter-spacing: 0.5px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 2.0625em;margin-bottom: 12px;text-wrap: wrap;\">\u7528\u4f4e\u65b9\u5dee\u6ee4\u6ce2\uff0c\u77ac\u95f4\u5c31\u628a\u7ef4\u5ea6\u4ece78\u964d\u5230\u4e8617\uff01\u662f\u4e0d\u662f\u5f88\u795e\u5947\u5462\uff1f\u5feb\u6765\u548c\u5c0f\u5e08\u59b9\u4e00\u8d77\u8bd5\u8bd5\u5427\uff01<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"text-indent: 2.0625em;margin-bottom: 12px;text-wrap: wrap;\">\u66f4\u591a\u5b9e\u7528\u65b9\u4fbf\u7684\u5c0f\u5de5\u5177\u5728\u4e91\u751f\u4fe1\u5e73\u53f0\u7b49\u7740\u4f60\u54e6\uff5e<\/p>\n<p style=\"text-indent: 2.0625em;text-wrap: wrap;\">http:\/\/www.biocloudservice.com\/home.html<\/p>\n<\/section>\n<\/section>\n<\/section>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_akXKU5NcO1KwuWc1Rk7ZJ56LC2pYrQ-6.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEXjjmsEC5aXz0RE4be2ib1fJPnBD8yX0Z6OF2zocd2ngHpLOakXKU5NcO1KwuWc1Rk7ZJ56LC2pYrQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_LzaFJVwzzLgfnj6Qf8lBiaTR4Pziag-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/oCSOtP61aEUEOPhTDbhztaU7davdEP2YWOAtToI143Aic2TkkStosJ9iahcnLzaFJVwzzLgfnj6Qf8lBiaTR4Pziag\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"895\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/v.xiumi.us\/board\/v5\/6rNpH\/474738576\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/v.xiumi.us\/board\/v5\/6rNpH\/474738576\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7b80\u5355\u7c97\u66b4\uff01\u4f4e\u65b9\u5dee\u6ee4\u6ce2VS\u9ad8\u76f8\u5173\u6ee4\u6ce2\uff0c\u54ea\u4e2a\u66f4\u7b80\u5355\uff1f\u4e00\u62db\u8ba9\u4f60\u7684\u6570\u636e\u53d8\u5f97\u8f7b\u677e\u65e0\u6bd4\uff01\uff08\u4e0a\uff09 \u5c0f\u5e08\u59b9 &nbs […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58136"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=58136"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58136\/revisions"}],"predecessor-version":[{"id":58144,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58136\/revisions\/58144"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=58136"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=58136"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=58136"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

{"id":58136,"date":"2024-06-16T22:27:57","date_gmt":"2024-06-16T14:27:57","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=58136"},"modified":"2024-06-16T22:27:57","modified_gmt":"2024-06-16T14:27:57","slug":"%e7%ae%80%e5%8d%95%e7%b2%97%e6%9a%b4%ef%bc%81%e4%bd%8e%e6%96%b9%e5%b7%ae%e6%bb%a4%e6%b3%a2vs%e9%ab%98%e7%9b%b8%e5%85%b3%e6%bb%a4%e6%b3%a2%ef%bc%8c%e5%93%aa%e4%b8%aa%e6%9b%b4%e7%ae%80%e5%8d%95%ef%bc%9f","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=58136","title":{"rendered":"\u7b80\u5355\u7c97\u66b4\uff01\u4f4e\u65b9\u5dee\u6ee4\u6ce2VS\u9ad8\u76f8\u5173\u6ee4\u6ce2\uff0c\u54ea\u4e2a\u66f4\u7b80\u5355\uff1f\u4e00\u62db\u8ba9\u4f60\u7684\u6570\u636e\u53d8\u5f97\u8f7b\u677e\u65e0\u6bd4\uff01\uff08\u4e0a\uff09"},"content":{"rendered":"

\n
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style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(246, 248, 247);padding: 25px;\">\n<section style=\"text-align: justify;letter-spacing: 0.5px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 2.0625em;margin-bottom: 12px;text-wrap: 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onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-05_\u300c\u8f6c\u300d\u7b80\u5355\u7c97\u66b4\uff01\u4f4e\u65b9\u5dee\u6ee4\u6ce2VS\u9ad8\u76f8\u5173\u6ee4\u6ce2\uff0c\u54ea\u4e2a\u66f4\u7b80\u5355\uff1f\u4e00\u62db\u8ba9\u4f60\u7684\u6570\u636e\u53d8\u5f97\u8f7b\u677e\u65e0\u6bd4\uff01\uff08\u4e0a\uff09\/4_CiaN7s0q9HZZaRlJ3BoSMR3nbR2Bkw.png\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-05_\u300c\u8f6c\u300d\u7b80\u5355\u7c97\u66b4\uff01\u4f4e\u65b9\u5dee\u6ee4\u6ce2VS\u9ad8\u76f8\u5173\u6ee4\u6ce2\uff0c\u54ea\u4e2a\u66f4\u7b80\u5355\uff1f\u4e00\u62db\u8ba9\u4f60\u7684\u6570\u636e\u53d8\u5f97\u8f7b\u677e\u65e0\u6bd4\uff01\uff08\u4e0a\uff09\/5_CiaN7s0q9HZZaRlJ3BoSMR3nbR2Bkw.png\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-05_\u300c\u8f6c\u300d\u7b80\u5355\u7c97\u66b4\uff01\u4f4e\u65b9\u5dee\u6ee4\u6ce2VS\u9ad8\u76f8\u5173\u6ee4\u6ce2\uff0c\u54ea\u4e2a\u66f4\u7b80\u5355\uff1f\u4e00\u62db\u8ba9\u4f60\u7684\u6570\u636e\u53d8\u5f97\u8f7b\u677e\u65e0\u6bd4\uff01\uff08\u4e0a\uff09\/7_CiaN7s0q9HZZaRlJ3BoSMR3nbR2Bkw.png\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-05_\u300c\u8f6c\u300d\u7b80\u5355\u7c97\u66b4\uff01\u4f4e\u65b9\u5dee\u6ee4\u6ce2VS\u9ad8\u76f8\u5173\u6ee4\u6ce2\uff0c\u54ea\u4e2a\u66f4\u7b80\u5355\uff1f\u4e00\u62db\u8ba9\u4f60\u7684\u6570\u636e\u53d8\u5f97\u8f7b\u677e\u65e0\u6bd4\uff01\uff08\u4e0a\uff09\/8_CiaN7s0q9HZZaRlJ3BoSMR3nbR2Bkw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEWvVqOBicHvFuDE5TR9k3Dk6lH2zXemAzicuLTokHibcUiaOrMNeCiaN7s0q9HZZaRlJ3BoSMR3nbR2Bkw\/640?wx_fmt=png''''''\" data-type=\"png\" data-w=\"500\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;align-self: flex-end;flex: 100 100 0%;height: auto;\">\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 7px;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(93, 91, 92);height: 3px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(246, 248, 247);padding: 25px;\">\n<section style=\"text-align: justify;letter-spacing: 0.5px;\" powered-by=\"xiumi.us\">\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">\u4eca\u5929\u5c0f\u5e08\u59b9\u4ee5\u86cb\u767d\u8d28\u8868\u8fbe\u6570\u636e\u4e3a\u4f8b\uff0c\u5e26\u5927\u5bb6\u4e00\u8d77\u8bd5\u8bd5\u5427\uff5e<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">\u9996\u5148\u5bfc\u5165\u6570\u636e\uff0c\u5220\u9664\u7f3a\u5931\u503c<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">library(limma)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">library(edgeR)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">data=read.csv(file.choose(),header = TRUE)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">data <- na.omit(data)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">\u5728\u539f\u59cb\u6570\u636e\u4e2d\u4e0d\u540c\u57fa\u56e0\u5c5e\u4e8e\u4e0d\u540c\u5217\uff0c\u4e3a\u4e86\u65b9\u4fbf\u64cd\u4f5c\uff0c\u9700\u8981\u5bf9\u6570\u636e\u8fdb\u884c\u8f6c\u7f6e<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">X <- data[,2:79]<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"># \u4e3adata\u8bbe\u7f6e\u65b0\u5217<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">X$Genotype <- ifelse(X$Genotype == “Control”, 1, 0)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">Xt <- t(X)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">\u4f7f\u7528filterByExpr\u51fd\u6570\u8fdb\u884c\u4f4e\u65b9\u5dee\u6ee4\u6ce2\uff0c\u53bb\u6389\u8868\u8fbe\u91cf\u5c0f\u4e8e1\u7684\u57fa\u56e0<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">keep <- filterByExpr(Xt,group = Xt[78,], min.count=1)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">X_filtered <- Xt[keep, ]<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">\u67e5\u770b\u6ee4\u6ce2\u524d\u540e\u57fa\u56e0\u6570<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">cat(“Number of genes before filtering:”, nrow(Xt), “n”)<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\">cat(“Number of genes after filtering:”, nrow(X_filtered), “n”)<\/p>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.12734082397003746\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_aYa9jJpkd4ImzBUAfIiczen2QHpZFQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEWvVqOBicHvFuDE5TR9k3Dk6qN0HSPibGE6Vld4joNnrrOeQLiciaYa9jJpkd4ImzBUAfIiczen2QHpZFQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1068\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(246, 248, 247);padding: 25px;\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 100 100 0%;height: auto;\">\n<section style=\"overflow: hidden;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;\">\n<section style=\"transform: rotate(0.1deg);\">\n<section style=\"width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 100%;margin-right: -6px;margin-left: -7px;border-bottom: 2px dotted rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;align-self: center;padding-right: 32px;padding-left: 32px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;transform: translate3d(11px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;background-color: rgb(93, 91, 92);min-width: 5%;flex: 0 0 auto;height: auto;align-self: stretch;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;transform: translate3d(-22px, 0px, 0px);margin-top: 4px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"text-align: center;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 42px;height: auto;\"><img decoding=\"async\" data-ratio=\"0.986\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_cE6XdQiasqicVAIAia5LHVEXScnBfw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEWvVqOBicHvFuDE5TR9k3Dk6tGXWuiapfpFrdmg6hiaffco2EAxRricE6XdQiasqicVAIAia5LHVEXScnBfw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"500\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;min-width: 5%;flex: 0 0 auto;height: auto;padding-left: 14px;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(255, 255, 255);\">\n<p><strong>\u6700\u540e<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;height: auto;align-self: center;\">\n<section style=\"overflow: hidden;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;\">\n<section style=\"transform: rotate(0.1deg);\">\n<section style=\"width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 100%;margin-right: -6px;margin-left: -7px;border-bottom: 2px dotted rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 6px;height: 6px;border-radius: 100%;background-color: rgb(93, 91, 92);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;letter-spacing: 0.5px;\" powered-by=\"xiumi.us\">\n<p style=\"text-indent: 2.0625em;margin-bottom: 12px;text-wrap: wrap;\">\u7528\u4f4e\u65b9\u5dee\u6ee4\u6ce2\uff0c\u77ac\u95f4\u5c31\u628a\u7ef4\u5ea6\u4ece78\u964d\u5230\u4e8617\uff01\u662f\u4e0d\u662f\u5f88\u795e\u5947\u5462\uff1f\u5feb\u6765\u548c\u5c0f\u5e08\u59b9\u4e00\u8d77\u8bd5\u8bd5\u5427\uff01<\/p>\n<p style=\"margin-bottom: 12px;text-wrap: wrap;\"><br \/><\/p>\n<p style=\"text-indent: 2.0625em;margin-bottom: 12px;text-wrap: wrap;\">\u66f4\u591a\u5b9e\u7528\u65b9\u4fbf\u7684\u5c0f\u5de5\u5177\u5728\u4e91\u751f\u4fe1\u5e73\u53f0\u7b49\u7740\u4f60\u54e6\uff5e<\/p>\n<p style=\"text-indent: 2.0625em;text-wrap: wrap;\">http:\/\/www.biocloudservice.com\/home.html<\/p>\n<\/section>\n<\/section>\n<\/section>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_akXKU5NcO1KwuWc1Rk7ZJ56LC2pYrQ-6.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEXjjmsEC5aXz0RE4be2ib1fJPnBD8yX0Z6OF2zocd2ngHpLOakXKU5NcO1KwuWc1Rk7ZJ56LC2pYrQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.35083798882681566\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_LzaFJVwzzLgfnj6Qf8lBiaTR4Pziag-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/oCSOtP61aEUEOPhTDbhztaU7davdEP2YWOAtToI143Aic2TkkStosJ9iahcnLzaFJVwzzLgfnj6Qf8lBiaTR4Pziag\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"895\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/v.xiumi.us\/board\/v5\/6rNpH\/474738576\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/v.xiumi.us\/board\/v5\/6rNpH\/474738576\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7b80\u5355\u7c97\u66b4\uff01\u4f4e\u65b9\u5dee\u6ee4\u6ce2VS\u9ad8\u76f8\u5173\u6ee4\u6ce2\uff0c\u54ea\u4e2a\u66f4\u7b80\u5355\uff1f\u4e00\u62db\u8ba9\u4f60\u7684\u6570\u636e\u53d8\u5f97\u8f7b\u677e\u65e0\u6bd4\uff01\uff08\u4e0a\uff09 \u5c0f\u5e08\u59b9 &nbs […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58136"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=58136"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58136\/revisions"}],"predecessor-version":[{"id":58144,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58136\/revisions\/58144"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=58136"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=58136"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=58136"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}