{"id":58194,"date":"2024-06-16T22:31:57","date_gmt":"2024-06-16T14:31:57","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=58194"},"modified":"2024-06-16T22:31:57","modified_gmt":"2024-06-16T14:31:57","slug":"%e6%83%b3%e6%8f%90%e9%ab%98%e5%8d%95%e7%bb%86%e8%83%9e%e6%b3%a8%e9%87%8a%e5%87%86%e7%a1%ae%e5%ba%a6%ef%bc%9f%e6%89%8b%e5%8a%a8%e6%a0%a1%e6%ad%a3%e9%aa%8c%e8%af%81%e5%88%86%e6%9e%90-3","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=58194","title":{"rendered":"\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01"},"content":{"rendered":"


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target=\"_blank\" rel=\"noopener\">#\u5355\u7ec6\u80de\u6d4b\u5e8f<\/a><\/p>\n<\/div>\n<p style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\" data-mpa-powered-by=\"yiban.io\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100015224\" data-ratio=\"0.2222222222222222\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/1_r0Gd7iaumIGMiaia97aHFV15nrpdzw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxQW8Zek7Noib2uBhB3ZE3yMxI2Hfic7cGTdPYiacicg47jficdr5BVr0Gd7iaumIGMiaia97aHFV15nrpdzw\/640?wx_fmt=png&from=appmsg'\" data-type=\"png\" data-w=\"900\" style=\"\" \/><\/p>\n<p style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: 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none;\">\u57fa\u51c6\u7814\u7a76\u8868\u660e\uff0c\u81ea\u52a8\u6ce8\u91ca\u5de5\u5177\u7684\u6027\u80fd\u662f\u5904\u4e8e\u53d8\u5316\u4e2d\u7684\uff0c\u5b83\u7684\u7cbe\u51c6\u5ea6\u53d6\u51b3\u4e8e\u88ab\u6ce8\u91ca\u7ec6\u80de\u7c7b\u578b\u7684\u6570\u636e\u96c6\u548c\u57fa\u56e0\u8868\u8fbe\u8c31\u7684\u72ec\u7279\u6027\uff0c\u6bd4\u5982\u8bf4\uff0c\u4f7f\u7528\u81ea\u52a8\u6ce8\u91ca\u533a\u5206T\u7ec6\u80de\u548cB\u7ec6\u80de\u76f8\u5bf9\u7b80\u5355\uff0c\u4f46\u4f7f\u7528\u81ea\u52a8\u6ce8\u91ca\u5de5\u5177\u533a\u5206CD8+\u7ec6\u80de\u6bd2\u6027T\u7ec6\u80de\u548c\u81ea\u7136\u6740\u4f24\u7ec6\u80de\u5374\u6bd4\u8f83\u56f0\u96be\u3002\u56e0\u6b64\uff0c\u6211\u4eec\u9700\u8981\u5bf9\u81ea\u52a8\u6ce8\u91ca\u8fdb\u884c\u624b\u52a8\u6539\u8fdb\u4ee5\u53ca\u9a8c\u8bc1\u5206\u6790\uff0c\u4ece\u800c\u8ba9\u6211\u4eec\u7684\u6ce8\u91ca\u7ed3\u679c\u66f4\u52a0\u51c6\u786e\u53ef\u4fe1\u3002<\/span><span style=\"letter-spacing: 1px;font-size: 16px;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);\">\u672c\u6b21\u4ee3\u7801\u7684\u6570\u636e\u91cf\u504f\u5927\uff0c\u6b22\u8fce\u5927\u5bb6\u6765\u79df\u8d41\u6211\u4eec\u7684\u670d\u52a1\u5668\u54e6\uff0c\u5173\u6ce8\u516c\u4f17\u53f7\u8fd8\u53ef\u4ee5\u83b7\u5f97\u72ec\u7279\u7684\u751f\u4fe1\u5206\u6790\u601d\u8def\u5b9a\u5236~<\/span><\/span><\/p>\n<section style=\"font-size: 15px;padding-right: 2px;padding-left: 2px;margin-bottom: 0px;\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;\">\n<section style=\"display: flex;width: 100%;flex-flow: column;\" powered-by=\"xiumi.us\">\n<section style=\"z-index: 1;transform: perspective(0px);transform-style: flat;\" powered-by=\"xiumi.us\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;transform: translate3d(4px, 0px, 0px) rotateX(180deg);margin-bottom: -2px;\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 10px;height: 14px;vertical-align: top;overflow: hidden;background-color: rgb(91, 91, 91);border-style: solid;border-width: 2px;border-color: rgb(255, 255, 255);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 10px;height: 14px;vertical-align: top;overflow: hidden;background-color: rgb(91, 91, 91);border-style: solid;border-width: 2px;border-color: rgb(255, 255, 255);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(194, 209, 255);\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;margin-top: -4px;margin-bottom: 5px;transform: translate3d(5px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(193, 209, 255);padding: 10px 20px;box-shadow: rgb(255, 255, 255) -2px 2px 0px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;align-self: center;flex: 0 0 auto;width: 100%;height: auto;\">\n<section style=\"text-align: center;color: rgb(0, 0, 0);\" powered-by=\"xiumi.us\">\n<p><strong>\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c111\u201d<\/strong><\/p>\n<p><strong>\u9886\u53d6\u672c\u7bc7\u4ee3\u7801\u3001\u57fa\u56e0\u96c6\u6216\u793a\u4f8b\u6570\u636e\u7b49\u6587\u4ef6<\/strong><\/p>\n<p><strong>\u6587\u4ef6\u7f16\u53f7\uff1a240124<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><span style=\"color: rgb(171, 25, 66);\"><strong>\u9700\u8981\u79df\u8d41\u670d\u52a1\u5668\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u626b\u7801\u6dfb\u52a0\u5c0f\u679c\uff0c\u6b64\u5916\u5c0f\u679c\u8fd8\u63d0\u4f9b\u751f\u4fe1\u5206\u6790\uff0c\u601d\u8def\u8bbe\u8ba1\uff0c\u6587\u732e\u590d\u73b0\u7b49\uff0c\u6709\u9700\u8981\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u64a9~<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100015748\" data-ratio=\"1\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/2_f6PG40Qrdu5e9B1iaIMItPiajABYWA.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxqaqPbQprPd4vw8Ytp0cprbzXiakMDdFriaB6B59ibhlBCrNyQFf6PG40Qrdu5e9B1iaIMItPiajABYWA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"430\" style=\"width: 170px;height: 170px;\" \/><\/p>\n<\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"letter-spacing: 1px;font-size: 16px;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;\">\u63a5\u4e0b\u6765\u5c0f\u679c\u5c06\u4ece\u5934\u5f00\u59cb\uff0c\u5e26\u7740\u5c0f\u4f19\u4f34\u4eec\u4e00\u8d77\u5b8c\u6210\u5355\u7ec6\u80de\u6ce8\u91ca\u7684\u6574\u4e2a\u8fc7\u7a0b~\u672c\u6b21\u6559\u7a0b\u6211\u4eec\u4f7f\u7528\u7684\u662f\u7ec6\u80de\u662f\uff1aPeripheral blood mononuclear cells\uff08PBMC\uff09\u3002\u8fd9\u662f\u5916\u5468\u8840\u4e2d\u5177\u6709\u5355\u4e2a\u6838\u7684\u7ec6\u80de\u7684\u6df7\u5408\u7fa4\u4f53\uff0c\u662f\u514d\u75ab\u7ec6\u80de\u7684\u4e30\u5bcc\u6765\u6e90\uff0c\u53ef\u4ee5\u8fdb\u4e00\u6b65\u7eaf\u5316\u5206\u79bb\u51fa\u591a\u4e2a\u7ec6\u80de\u7c7b\u578b\u3002<\/span>    <br \/><\/span><\/section>\n<table style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;width: 100%;\">\n<tbody>\n<tr>\n<td style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;text-align: justify;\"><br \/><\/td>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\">\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015419\" data-ratio=\"0.7287037037037037\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/3_FlxSmATUuxfBicgeJzmZY9ZEX4yXeA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP64FGgZpczzQo8bEOR97WbgoibgeFlxSmATUuxfBicgeJzmZY9ZEX4yXeA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"314.0\" width=\"426.010009765625\" \/><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">         <\/span><\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"letter-spacing: 1px;font-size: 16px;text-indent: 2em;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;\">\u5982\u56fe\uff0c\u662f\u672c\u6b21\u6240\u9700\u5f97\u57fa\u56e0\u6587\u4ef6\u3002\u53e6\u5916\u672c\u6b21\u5206\u6790\u6240\u9700\u7684R\u7248\u672c\u4ee5\u53caR\u5305\u7248\u672c\u5c0f\u679c\u4e5f\u5e2e\u5927\u5bb6\u6574\u7406\u597d\u4e86~<\/span><span style=\"letter-spacing: 1px;font-size: 16px;text-indent: 2em;\">       <\/span><\/span><\/section>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015551\" data-ratio=\"0.24166666666666667\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/4_Ovfibzr8mKMX4fcREDAPIBa0bvK1rQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP6viaulNbgWiaxHKWX7Qyv5ic34SkqOvfibzr8mKMX4fcREDAPIBa0bvK1rQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"133.66998291015625\" style=\"letter-spacing: 0.578px;white-space: normal;\" width=\"553.739990234375\" \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cpp\"><code><span class=\"code-snippet_outer\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">Step 1 \u5b89\u88c5R\u5305install.packages(c(\"BiocManager\",\"Seurat\",\"SCINA\",\"ggplot2\",\"devtools\",\"shiny\")) BiocManager::install(c(\"SingleCellExperiment\",\"scater\",\"dplyr\", \"scmap\",\"celldex\",\"SingleR\",\"GSVA\",\"S4Vectors\", \"SummarizedExperiment\"))devtools::install_github(\"immunogenomics\/harmony\")devtools::install_github(\"romanhaa\/cerebroApp\")devtools::install_github(\"mw201608\/msigdb\")<\/span><\/span><\/code><\/pre>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">Step 2 \u57fa\u4e8e\u53c2\u8003\u7684\u81ea\u52a8\u6ce8\u91ca<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">#\u4eceGEO\u6570\u636e\u5e93\u4e0b\u8f7d\u6570\u636e\uff08\u7531\u4e8e\u5df2\u7ecf\u4e0b\u8f7d\u597d\u4e86\u6240\u4ee5\u76f4\u63a5\u5bfc\u5165\u6587\u4ef6\u5373\u53ef\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">set.seed(9742)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">ref <- readRDS(\"..\/data\/data_DICE.RData\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\uff03\u683c\u5f0f\u5316\u6570\u636e\uff0c\u786e\u4fdd\u4e0e\u6b63\u5728\u4f7f\u7528\u7684\u5de5\u5177\u517c\u5bb9\uff0c\u8fd9\u91cc\u4f7f\u7528\u7684\u662fscmap<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u5728\u540d\u4e3a\u201ccell_type1\u201d\u7684\u5217\u4e2d\u5206\u914d\u5355\u5143\u683c\u7c7b\u578b\u6807\u7b7e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">SummarizedExperiment::colData(ref)$cell_type1 <- SummarizedExperiment::colData(ref)$label.fine<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u5728\u540d\u4e3a\u201cfeature_symbol\u201d\u7684\u5217\u4e2d\u5206\u914d\u57fa\u56e0\u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">SummarizedExperiment::rowData(ref)$feature_symbol <- rownames(ref)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u5c06\u6570\u636e\u8f6c\u6362\u4e3aSingleCellExperiment\u5bf9\u8c61<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">ref_sce <- SingleCellExperiment::SingleCellExperiment(assays=list(logcounts=Matrix::Matrix(SummarizedExperiment::assays(ref)$logcounts)), <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> colData=SummarizedExperiment::colData(ref),<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> rowData=SummarizedExperiment::rowData(ref))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/section><\/pre>\n<\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(0, 112, 192);font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><o:p><\/o:p><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8fd9\u6837\u53c2\u8003\u6570\u636e\u5c31\u53ef\u4ee5\u4f7f\u7528\u4e86\uff0c\u7136\u540e\u6211\u4eec\u9700\u8981\u5904\u7406\u8fd9\u4e9b\u6570\u636e\u6765\u6784\u5efa\u5c06\u7528\u6765\u5c06\u672a\u6807\u8bb0\u6570\u636e\u6620\u5c04\u7684\u7d22\u5f15\u3002\u9996\u5148\uff0c\u6211\u4eec\u9009\u62e9\u8981\u4f7f\u7528\u7684\u57fa\u56e0\uff0c\u8fd9\u4e9b\u57fa\u56e0\u5c06\u662f\u5728\u901a\u8fc7\u57fa\u56e0\u4e22\u5931\u5206\u5e03\u5c06\u7ebf\u6027\u6a21\u578b\u62df\u5408\u5230\u57fa\u56e0\u8868\u8fbe\u540e\u88ab scmap \u68c0\u7d22\u7684\u8ba4\u4e3a\u6700\u5177\u4fe1\u606f\u6027\u7684\u57fa\u56e0\uff0c\u4fe1\u606f\u6700\u4e30\u5bcc\u7684\u7ec6\u80de\u5177\u6709\u9ad8\u8868\u8fbe\u503c\u548c\u4f4e\u4e22\u5931\u7387\u3002<\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">#\u9996\u5148\u9009\u62e9\u4fe1\u606f\u6700\u4e30\u5bcc\u7684\u7279\u5f81\u6765\u521b\u5efa scmap-cluster \u53c2\u8003ref_sce <- scmap::selectFeatures(ref_sce, suppress_plot=FALSE)<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<section style=\"text-align: center;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015562\" data-ratio=\"0.6222222222222222\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/5_9aThmzWRq2VwtmJLtofXLKib7V4eRw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP6yJC9ZMvY2xztic0Ra1jUEgdM7D9aThmzWRq2VwtmJLtofXLKib7V4eRw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"318.6700439453125\" style=\"letter-spacing: 0.578px;text-indent: 34px;white-space: normal;\" width=\"512.5399780273438\" \/><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"letter-spacing: 1px;font-size: 16px;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;\">\u5982\u56fe\u662f\u901a\u8fc7\u53ef\u89c6\u5316\u6765\u5c55\u793a\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u7684\u8d28\u91cf\u63a7\u5236\uff0c\u7279\u5f81\u9009\u62e9\u7684\u7ed3\u679c\u3002log2(Expression)\u8868\u793a\u6bcf\u4e2a\u7279\u5f81\u7684\u5e73\u5747\u8868\u8fbe\u6c34\u5e73\uff0clog2(% of dropouts)\u8868\u793a\u6bcf\u4e2a\u7279\u5f81\u5728\u591a\u5c11\u6bd4\u4f8b\u7684\u7ec6\u80de\u4e2d\u6ca1\u6709\u88ab\u68c0\u6d4b\u5230\u3002\u56fe\u4e2d\u7684\u7ea2\u8272\u70b9\u5c31\u662f\u6839\u636e\u6807\u51c6\u7b97\u6cd5\u9009\u62e9\u51fa\u6765\u7684\u7279\u5f81\uff0c\u800c\u84dd\u8272\u70b9\u662f\u88ab\u6392\u9664\u7684\u7279\u5f81\u3002<\/span>    <span style=\"letter-spacing: 1px;font-size: 16px;\">        <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> <\/span><\/o:p><br \/><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><o:p style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 1px;\"><\/o:p><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">#\u68c0\u67e5 scmap \u9009\u62e9\u7684\u524d 50 \u4e2a\u57fa\u56e0rownames(ref_sce)[which(SummarizedExperiment::rowData(ref_sce)$scmap_features)][1:50]<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><o:p><\/o:p><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"letter-spacing: 1px;font-size: 16px;font-variant: normal;text-transform: none;\">\u5982\u56fe\uff0c\u662f\u6211\u4eec\u7b5b\u9009\u7684\u524d<\/span>          <\/span><\/section>\n<section style=\"text-align: center;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015554\" data-ratio=\"0.3430232558139535\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/6_oLPECemPrXg9FmDv6TdUMCJhqeLwwg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP6cuDu9aV5EkP4QSKuPIQOJtKroLPECemPrXg9FmDv6TdUMCJhqeLwwg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"860\" height=\"189.94000244140625\" style=\"letter-spacing: 0.578px;white-space: normal;\" width=\"553.739990234375\" \/><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">50\u4e2a\u57fa\u56e0<\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">#\u901a\u8fc7\u68c0\u67e5\u57fa\u56e0\u540d\u79f0\u5411\u91cf\u7684\u957f\u5ea6\u6765\u68c0\u67e5\u9009\u62e9\u4e86\u591a\u5c11\u4e2a\u57fa\u56e0length(rownames(ref_sce)[which(SummarizedExperiment::rowData(ref_sce)$scmap_features)])<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015552\" data-ratio=\"0.05277777777777778\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/7_rhXFHIfcicTugFVkfvGKd8LK9bO00A.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP6HmHOcibFpDDVTPB5udBJ2ZuIWibrhXFHIfcicTugFVkfvGKd8LK9bO00A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"29.07000732421875\" style=\"letter-spacing: 0.578px;white-space: normal;\" width=\"553.739990234375\" \/><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"letter-spacing: 1px;font-size: 16px;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;\">\u73b0\u5728\u6211\u4eec\u53ef\u4ee5\u770b\u5230 scmap \u9009\u62e9\u4f7f\u7528\u7684\u57fa\u56e0\u3002\u5982\u679c\u7f3a\u5c11\u5173\u952e\u6807\u8bb0\u57fa\u56e0\uff0c\u6211\u4eec\u53ef\u4ee5\u786e\u4fdd\u5c06\u5b83\u4eec\u5305\u542b\u5728\u5185\uff0c\u5982\u4e0b\u6240\u793a\uff1a<\/span>    <\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">#\u521b\u5efa\u8981\u4f7f\u7528\u7684\u5173\u952e\u6807\u8bb0\u7684\u5217my_key_markers = c(\"TRAC\", \"TRBC1\", \"TRBC2\", \"TRDC\", \"TRGC1\", \"TRGC2\", \"IGKC\")# \u786e\u4fdd\u6807\u8bb0\u4f4d\u4e8e scmap \u4f7f\u7528\u7684\u529f\u80fd\u5217\u8868\u4e2dSummarizedExperiment::rowData(ref_sce)$scmap_features[rownames(ref_sce) %in% my_key_markers] <- TRUE# \u901a\u8fc7\u518d\u6b21\u68c0\u67e5\u57fa\u56e0\u540d\u79f0\u5411\u91cf\u7684\u957f\u5ea6\u6765\u68c0\u67e5\u662f\u5426\u6dfb\u52a0\u4e86\u4efb\u4f55\u57fa\u56e0\u957f\u5ea6length(rownames(ref_sce)[which(SummarizedExperiment::rowData(ref_sce)$scmap_features)])<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015553\" data-ratio=\"0.044444444444444446\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/8_ascdtV60mq0fibiaRlicJXYqT8H2Iw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP6oSibOnv9c7GhEB5IaRPyYXmpokGcascdtV60mq0fibiaRlicJXYqT8H2Iw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"24.79998779296875\" style=\"letter-spacing: 0.578px;white-space: normal;\" width=\"553.739990234375\" \/><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u6211\u4eec\u53ef\u4ee5\u5220\u9664\u53ef\u80fd\u662f\u6280\u672f\u4ea7\u7269\u7684\u57fa\u56e0\uff0c\u4f8b\u5982\u7ebf\u7c92\u4f53 RNA\uff0c\u5982\u4e0b\u6240\u793a\uff1a<\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u4ece\u6570\u636e\u96c6\u4e2d\u521b\u5efa\u7ebf\u7c92\u4f53\u57fa\u56e0\u5217\u8868\uff08\u4ee5\u201cMT\u201d\u5f00\u5934\u7684\u57fa\u56e0\uff09mt_genes <- rownames(ref_sce)[grep(\"^MT-\", rownames(ref_sce))]# \u4ece scmap \u4f7f\u7528\u7684\u7279\u5f81\u4e2d\u5220\u9664\u8fd9\u4e9b\u57fa\u56e0SummarizedExperiment::rowData(ref_sce)$scmap_features[rownames(ref_sce) %in% mt_genes] <- FALSE# \u68c0\u67e5\u6709\u591a\u5c11\u4e2a\u57fa\u56e0length(rownames(ref_sce)[which(SummarizedExperiment::rowData(ref_sce)$scmap_features)])<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015555\" data-ratio=\"0.04722222222222222\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/9_TAmsPapetUhjpRkDAEGQWVznN610VA.png\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/10_TAmsPapetUhjpRkDAEGQWVznN610VA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP6fdgE1mECicYZJC1pCnYFARuK2TAmsPapetUhjpRkDAEGQWVznN610VA\/640?wx_fmt=png''\" data-type=\"png\" data-w=\"1080\" height=\"26.07000732421875\" style=\"letter-spacing: 0.578px;white-space: normal;\" width=\"553.739990234375\" \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><section style=\"margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">#\u63d0\u53d6\u7279\u5f81\u5e76\u5c06\u5176\u5206\u914d\u7ed9\u65b0\u53d8\u91cf\u201cscmap_feature_genes\u201dscmap_feature_genes <- rownames(ref_sce)[which(SummarizedExperiment::rowData(ref_sce)$scmap_features)]#\u57fa\u56e0\/\u7279\u5f81\u7684\u6570\u91cf\u4e0e\u6211\u4eec\u521a\u521a\u68c0\u67e5\u7684\u76f8\u540clength(scmap_feature_genes)<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<table style=\"width: 100%;\">\n<tbody>\n<tr>\n<td width=\"0.0\" height=\"20.600313\" style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;text-align: justify;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015556\" data-ratio=\"0.04722222222222222\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/9_TAmsPapetUhjpRkDAEGQWVznN610VA.png\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/10_TAmsPapetUhjpRkDAEGQWVznN610VA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP6fdgE1mECicYZJC1pCnYFARuK2TAmsPapetUhjpRkDAEGQWVznN610VA\/640?wx_fmt=png''\" data-type=\"png\" data-w=\"1080\" height=\"26.07000732421875\" width=\"553.739990234375\" style=\"letter-spacing: 0.578px;white-space: normal;\" \/><\/td>\n<td width=\"0.0\" height=\"20.600313\" style=\"border-width: initial;border-style: none;border-color: initial;text-align: justify;\"><br \/><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u73b0\u5728\u6211\u4eec\u6784\u5efa scmap-cluster \u4e2d\u4f7f\u7528\u7684\u53c2\u8003\u914d\u7f6e\u6587\u4ef6\uff0c\u7528\u4e8e\u57fa\u4e8e\u7c07\u7684\u7ec6\u80de\u7c7b\u578b\u6ce8\u91ca\u3002\u6211\u4eec \u53ef\u4ee5\u4ece SingleCellExperiment \u5bf9\u8c61\u5185\u90e8\u8bbf\u95ee\u548c\u7ed8\u5236\u8fd9\u4e9b\u914d\u7f6e\u6587\u4ef6\uff0c\u5982\u4e0b\u6240\u793a\uff1a<\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u521b\u5efa\u53c2\u8003\u8d44\u6599\uff1b# \u751f\u6210\u53c2\u8003\u914d\u7f6e\u6587\u4ef6\u540e\uff0cscmap-cluster \u4e0d\u9700\u8981\u539f\u59cb\u6570\u636eref_sce <- scmap::indexCluster(ref_sce)# \u5c06\u529f\u80fd\u53ef\u89c6\u5316\u4e3a\u70ed\u56fe# \u91cd\u65b0\u683c\u5f0f\u5316\u6570\u636e\uff0c\u4ee5\u4fbf\u5b83\u4eec\u53ef\u4ee5\u7528\u4f5c ggplot2 \u7684\u8f93\u5165cormat <- reshape2::melt(as.matrix(S4Vectors::metadata(ref_sce)$scmap_cluster_index))# \u7ed8\u56fe ggplot2::ggplot(cormat, ggplot2::aes(x = Var2, y = Var1, fill = value)) + ggplot2::geom_tile() + ggplot2::scale_fill_gradient2(low = \"blue\", high = \"darkred\", name = \"Expression value\") + ggplot2::theme_minimal() + ggplot2::theme(axis.text.x = ggplot2::element_text(angle = 90, vjust = 1, size = 18, hjust = 1), axis.text.y = ggplot2::element_text(size = 15), axis.title.x = ggplot2::element_blank(), axis.title.y = ggplot2::element_blank())<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015561\" data-ratio=\"0.9854014598540146\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/11_Tx5NpLGqvHvQuicibTDIWrv2Y7XxrA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP69pphs9K0HpiaoH3pAicD6icx8ne12Tx5NpLGqvHvQuicibTDIWrv2Y7XxrA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"959\" height=\"411.8699951171875\" style=\"letter-spacing: 0.578px;text-indent: 34px;white-space: normal;\" width=\"418.010009765625\" \/><\/p>\n<p><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;\">\u7ed3\u679c\u5982\u56fe\u6240\u793a\uff0c<\/span><span style=\"font-size: 16px;letter-spacing: 1px;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;color: rgb(17, 17, 17);\">\u8fd9\u4e2a\u56fe\u7247\u663e\u793a\u4e86\u4e0d\u540c\u7c7b\u578b\u7684\u7ec6\u80de\u4e0e\u7279\u5b9a\u57fa\u56e0\u4e4b\u95f4\u7684\u5173\u7cfb\u3002\u989c\u8272\u7684\u6df1\u6d45\u8868\u793a\u5173\u8054\u7a0b\u5ea6\u6216\u6d3b\u52a8\u6c34\u5e73\u7684\u9ad8\u4f4e\u3002\u5c0f\u679c\u8fd9\u91cc\u53ea\u622a\u53d6\u4e86\u4e00\u90e8\u5206\uff0c\u5b8c\u6574\u7684\u56fe\u7247\u5927\u5bb6\u53ef\u4ee5\u81ea\u5df1\u8bd5\u7740\u7ed8\u5236\u54e6~<\/span>    <span style=\"font-size: 16px;letter-spacing: 1px;color: rgb(17, 17, 17);\">         <\/span><\/span><\/p>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><o:p><\/o:p><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"letter-spacing: 1px;font-size: 16px;font-variant: normal;text-transform: none;color: rgb(17, 17, 17);\">\u63a5\u4e0b\u6765\u6211\u4eec\u7528scmap-cell \u6765\u6ce8\u91ca\u6570\u636e\u96c6\u4e2d\u7684\u5355\u4e2a\u7ec6\u80de\u3002<\/span><span style=\"letter-spacing: 1px;font-size: 16px;color: rgb(17, 17, 17);\"> <\/span><\/span><o:p style=\"color: rgb(17, 17, 17);font-family: Roboto;font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> <\/span><\/o:p><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u5c06\u8868\u8fbe\u5f0f\u4fe1\u606f\u5b58\u50a8\u4e3a\u53d8\u91cf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">scmap_cluster_reference <- S4Vectors::metadata(ref_sce)$scmap_cluster_index<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u66f4\u65b0\u4e4b\u524d\u7684\u5f15\u7528\u4ee5\u4e5f\u5305\u542b scmap-cell \u5f15\u7528<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">ref_sce <- scmap::indexCell(ref_sce)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">#\u4ece\u5f15\u7528\u4e2d\u63d0\u53d6 scmap \u7d22\u5f15\u5e76\u5c06\u5176\u5b58\u50a8\u4e3a\u53d8\u91cf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">scmap_cell_reference <- S4Vectors::metadata(ref_sce)$scmap_cell_index<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u4ece\u5f15\u7528\u4e2d\u63d0\u53d6\u5173\u8054\u7684\u7ec6\u80de ID \u5e76\u4fdd\u5b58\u4e3a\u53d8\u91cf<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">scmap_cell_metadata <- SummarizedExperiment::colData(ref_sce)<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"letter-spacing: 1px;font-size: 16px;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;\">\u7136\u540e\u5c06\u67e5\u8be2\u6570\u636e\u96c6\u4e2d\u7684\u7ec6\u80de\u5206\u914d\u7ed9\u53c2\u8003\u6570\u636e\u96c6\uff0c\u5229\u7528\u4e00\u4e2a\u5df2\u6709\u7684\u53c2\u8003\u6570\u636e\u96c6\u6765\u5bf9\u4e00\u4e2a\u65b0\u7684\u67e5\u8be2\u6570\u636e\u96c6\u8fdb\u884c\u6ce8\u91ca\u6216\u6620\u5c04\u3002<\/span><span style=\"letter-spacing: 1px;font-size: 16px;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;color: rgb(51, 51, 51);background: rgb(255, 255, 255);\">\u6211\u4eec\u5c06\u4f7f\u7528\u7684\u67e5\u8be2\u6570\u636e\u96c6\u7531 10X genomics \u63d0\u4f9b<\/span><span style=\"letter-spacing: 1px;font-size: 16px;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;\">\uff0c\u5927\u5bb6\u53ef\u4ee5\u4ece\u5c0f\u679c\u7ed9\u5927\u5bb6\u63d0\u4f9b\u7684\u94fe\u63a5\u4e0b\u8f7d~<\/span><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">data <- Seurat::Read10X(\"..\/data\/hg19\/\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u4ece\u539f\u59cb\u77e9\u9635\u4e2d\u8fdb\u884c SingleCellExperiment<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">query_sce <- SingleCellExperiment::SingleCellExperiment(assays=list(counts=data)) <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u4ece Seurat \u5bf9\u8c61\u5236\u4f5c SingleCellExperiment<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">query_seur <- Seurat::CreateSeuratObject(data)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">query_sce <- Seurat::as.SingleCellExperiment(query_seur)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u4f7f\u7528 scatter \u5305\u6807\u51c6\u5316\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">query_sce <- scater::logNormCounts(query_sce)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u6dfb\u52a0feature_symbol\u5217\uff08\u5373\u57fa\u56e0\u7b26\u53f7\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">SummarizedExperiment::rowData(query_sce)$feature_symbol <- rownames(query_sce)<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> \u73b0\u5728\uff0c\u6211\u4eec\u5df2\u51c6\u5907\u597d\u4f7f\u7528 scmap-cluster \u5bf9\u6570\u636e\u8fdb\u884c\u6ce8\u91ca\u3002  <\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> <\/span><\/o:p><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u8fd0\u884c scmapCluster<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">scmap_cluster_res <- scmap::scmapCluster(projection=query_sce,<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">index_list=list(immune1 = scmap_cluster_reference),<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">threshold=0.1)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u7ed8\u5236\u6ce8\u91ca\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">par(mar=c(13, 4, 1, 0)) <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">barplot(table(scmap_cluster_res$combined_labs), las=2)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/section><\/pre>\n<\/section>\n<section style=\"text-align: center;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015550\" data-ratio=\"0.6650853889943074\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_cEwG382lmhuythxibV0iaEbkEx6dew.png?resize=459%2C305\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP69uczfPicnTS0NncNibiaBqa0BT95ibicEwG382lmhuythxibV0iaEbkEx6dew\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1054\" height=\"305 .5400085449219\" style=\"letter-spacing: 0.578px;white-space: normal;\" width=\"459 .34002685546875\" data-recalc-dims=\"1\" \/><\/section>\n<table style=\"width: 100%;\">\n<tbody>\n<tr>\n<td width=\"46.667377\" height=\"34.667194\" style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;text-align: justify;\"><br \/><\/td>\n<td width=\"46.667377\" height=\"34.667194\" style=\"border-width: initial;border-style: none;border-color: initial;text-align: justify;\"><br \/><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u4e0a\u56fe\u5c31\u662f\u6211\u4eec\u8fdb\u884c\u6ce8\u91ca\u7684\u7ed3\u679c\u5566\uff0c\u53ef\u4ee5\u770b\u5230Monocytes CD14+\u7ec6\u80de\u7684\u6570\u91cf\u662f\u6700\u591a\u7684\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u5c06\u6b64\u6ce8\u91ca\u4fe1\u606f\u5b58\u50a8\u5728\u67e5\u8be2\u5bf9\u8c61\u4e2d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">SummarizedExperiment::colData(query_sce)$scmap_cluster <- scmap_cluster_res$combined_labs<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u5236\u4f5c\u5355\u5143\u683c\u7684 UMAP\uff0c\u5e76\u4f7f\u7528 scmapCluster \u4e2d\u7684\u5355\u5143\u683c\u7c7b\u578b\u6ce8\u91ca\u8fdb\u884c\u6807\u8bb0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">query_sce <- scater::runUMAP(query_sce)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">scater::plotReducedDim(query_sce, dimred=\"UMAP\", colour_by=\"scmap_cluster\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/section><\/pre>\n<\/section>\n<section style=\"text-align: center;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015549\" data-ratio=\"0.687962962962963\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/13_cISU4bPbHK5982VynALQ0gn1U9UQNQ.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP6UcG90meCKiblvQKDGbGTsYVwwcISU4bPbHK5982VynALQ0gn1U9UQNQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"381.1400146484375\" style=\"letter-spacing: 0.578px;white-space: normal;\" width=\"553.739990234375\" \/><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u7ed3\u679c\u5982\u56fe\uff0c\u5c55\u793a\u4e86\u4e0d\u540c\u7c7b\u578b\u7684\u7ec6\u80de\u5728\u4e8c\u7ef4\u7a7a\u95f4\u4e2d\u7684\u5206\u5e03\u548c\u805a\u7c7b\u3002\u6bcf\u79cd\u989c\u8272\u4ee3\u8868\u4e00\u79cd\u7279\u5b9a\u7c7b\u578b\u7684\u7ec6\u80de\uff0c\u4f8b\u5982B\u7ec6\u80de\u3001T\u7ec6\u80de\u7b49\u3002<\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">          <o:p> <\/o:p><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(17, 17, 17);font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u9664\u6b64\u4e4b\u5916\uff0c\u6211\u4eec\u8fd8\u53ef\u4ee5\u7528scmap_cell\uff0csingleR\uff0cHarmony_lab\u7b49\u65b9\u5f0f\u8fdb\u884c\u81ea\u52a8\u6ce8\u91ca~<\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">Step 3 \u7ec6\u5316\u6ce8\u91ca\u5728\u8fd9\u91cc\u6211\u4eec\u4f7f\u7528\u8de8\u5de5\u5177\u7684\u6700\u5e38\u89c1\u6807\u7b7e\u6765\u5206\u914d\u6700\u7ec8\u7684\u81ea\u52a8\u6ce8\u91ca\u6807\u7b7e\u3002annotation_columns <- c(\"scmap_cluster\", \"scmap_cell\", \"singleR\", \"Harmony_lab\")get_consensus_label <- function(labels){ labels <- labels[labels != \"ambiguous\"]if (length(labels) == 0) {return(\"ambiguous\")} freq <- table(labels) label <- names(freq)[which(freq == max(freq))]if (length(label) > 1) {return(\"ambiguous\")}return(label)}SummarizedExperiment::colData(query_sce)$consensus_lab <- apply(SummarizedExperiment::colData(query_sce)[,annotation_columns], 1, get_consensus_label)scater::plotReducedDim(query_sce, dimred=\"UMAP\", colour_by=\"consensus_lab\")<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<table style=\"width: 100%;\">\n<tbody>\n<tr>\n<td style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;text-align: justify;\"><br \/><\/td>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\">\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015430\" data-ratio=\"0.7046296296296296\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/14_EGEdZkIFTVMfYVcSOEicHjYicADtsQ.png?resize=553%2C390\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP6yeQdLs2fl6GFgicCKo2twvxBqqBEGEdZkIFTVMfYVcSOEicHjYicADtsQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"390 .27001953125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><br \/>\n        <\/span><\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-size: 16px;letter-spacing: 1px;\">\u7ec6\u5316\u7684\u7ed3\u679c\u5982\u56fe\uff0c\u53ef\u4ee5\u4ece\u4fa7\u8fb9\u7684\u6807\u6ce8\u770b\u51fa\u6765\u660e\u663e\u6bd4\u4e4b\u524d\u7684\u5206\u7c7b\u66f4\u52a0\u7ec6\u81f4\u4e86\u3002<\/span><span style=\"font-size: 16px;letter-spacing: 1px;\">       <\/span><\/span><\/p>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><o:p><\/o:p><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;\">Step 4 \u624b\u52a8\u6ce8\u91ca<\/span><span style=\"font-size: 16px;letter-spacing: 1px;\">         <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 1px;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> <\/span><\/o:p><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><o:p><\/o:p><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;\">\u9996\u5148\u6211\u4eec\u9700\u8981\u68c0\u7d22\u6807\u8bb0\u57fa\u56e0\uff0c\u5982\u679c\u6ca1\u6709\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u6807\u8bb0\u7269\u7684\u5217\u8868\uff0c\u4e5f\u6ca1\u6709\u9ad8\u8d28\u91cf\u7684\u53c2\u8003\u6570\u636e\u96c6\u7684\u8bdd\uff0c\u53ef\u4ee5\u4ece\u67e5\u8be2\u6570\u636e\u7684\u6bcf\u4e2a\u805a\u7c7b\u4e2d\u63d0\u53d6\u9876\u7ea7\u6807\u8bb0\u57fa\u56e0\u3002\u8fd9\u91cc\u6211\u4eec\u4f7f\u7528 Seurat\u6765\u5b8c\u6210\uff0c\u5c06\u6570\u636e\u683c\u5f0f\u5316\u4e3aSeurat \u5bf9\u8c61\u3002\u5148\u5bf9\u6570\u636e\u8fdb\u884c\u5f52\u4e00\u5316\u548c\u7f29\u653e\uff0c\u5e76\u4e14\u786e\u5b9a\u7ec6\u80de\u4e4b\u95f4\u7684\u53ef\u53d8\u57fa\u56e0\u3002<\/span><span style=\"font-size: 16px;letter-spacing: 1px;\">     <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 1px;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> <\/span><\/o:p><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u4f7f\u7528 Seurat \u7684 SCTransform \u51fd\u6570\u7f29\u653e\u548c\u6807\u51c6\u5316\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">query_seur <- Seurat::SCTransform(query_seur)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/section><\/pre>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">#\u63a5\u4e0b\u6765\uff0c\u5bf9\u6570\u636e\u6267\u884c\u4e0d\u540c\u7c7b\u578b\u7684\u964d\u7ef4\uff0c\u4ee5\u4fbf\u53ef\u4ee5\u5728\u4e8c\u7ef4\u7a7a\u95f4\u4e2d\u5c06\u5355\u5143\u683c\u7ec4\u5408\u5728\u4e00\u8d77\u3002query_seur <- Seurat::RunPCA(query_seur)query_seur <- Seurat::RunTSNE(query_seur)query_seur <- Seurat::RunUMAP(query_seur, dims = 1:50)#\u4ee5\u9009\u5b9a\u7684\u5206\u8fa8\u7387\u5bf9\u6570\u636e\u8fdb\u884c\u805a\u7c7b# \u786e\u5b9a\u6bcf\u4e2a\u5355\u5143\u683c\u7684\u201c\u6700\u8fd1\u90bb\u5c45\u201dquery_seur <- Seurat::FindNeighbors(query_seur, dims = 1:50)# \u805a\u7c7bquery_seur <- Seurat::FindClusters(query_seur, resolution = 0.5)#\u53ef\u89c6\u5316Seurat::DimPlot(query_seur, reduction = \"UMAP\")<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<table style=\"width: 100%;\">\n<tbody>\n<tr>\n<td width=\"0.0\" height=\"7.20011\" style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;text-align: justify;\"><br \/><\/td>\n<td width=\"0.0\" height=\"7.20011\" style=\"border-width: initial;border-style: none;border-color: initial;text-align: justify;\"><br \/><\/td>\n<\/tr>\n<tr>\n<td style=\"border-width: initial;border-style: none;border-color: initial;text-align: justify;\"><br \/><\/td>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\">\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015428\" data-ratio=\"0.649074074074074\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/15_M0GlOicMa8zhictnDPXyoXWFmXtiaA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP6SJGyB7ZFbEHDkXImTyenMxfdFvrM0GlOicMa8zhictnDPXyoXWFmXtiaA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"359.4700012207031\" width=\"553.739990234375\" \/><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><br \/>\n        <\/span><\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5982\u56fe\uff0c\u662f\u8fd8\u6ca1\u6709\u63d0\u53d6\u6807\u8bb0\u57fa\u56e0\u7684\u521d\u6b65\u7ed8\u5236\u7684\u70ed\u56fe\uff0c\u63a5\u4e0b\u6765\u6211\u4eec\u63d0\u53d6\u9876\u90e8\u57fa\u56e0\u3002<br \/><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">#\u4f7f\u7528 Seurat \u4e2d\u7684 FindAllMarkers \u51fd\u6570\u6765\u5b8c\u6210markers_seur <- Seurat::FindAllMarkers(query_seur, only.pos = TRUE)# \u67e5\u770b\u6807\u8bb0\uff0c\u5b83\u4eec\u6309\u7167\u6bcf\u4e2a\u7c07\u7684 p \u503c\u4ece\u6700\u4f4e\u5230\u6700\u9ad8\u7684\u987a\u5e8f\u6392\u5217markers_seur        <\/span><\/span><\/code><\/pre>\n<\/section>\n<table style=\"width: 100%;\">\n<tbody>\n<tr>\n<td style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;text-align: justify;\"><br \/><\/td>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\">\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015427\" data-ratio=\"0.39444444444444443\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/16_X31mkp1BvGMlNK6oNnhou1snhSTttA.png?resize=553%2C218\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP67OtEicHzWqEnr8uSgSrWjSLCjX31mkp1BvGMlNK6oNnhou1snhSTttA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"218 .39999389648438\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><br \/>\n        <\/span><\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;letter-spacing: 1px;\">\u7ed3\u679c\u5982\u56fe\uff0c\u662f\u6211\u4eec\u6311\u9009\u7684\u6807\u8bb0\u57fa\u56e0\u3002\u4e0d\u540c\u7ec6\u80de\u7c07\u4e4b\u95f4\u7684\u6807\u8bb0\u57fa\u56e0\u7684\u8868\u8fbe\u901a\u5e38\u7528\u70b9\u56fe\u6216\u70ed\u56fe\u6765\u5c55\u793a\u3002\u70b9\u56fe\u663e\u793a\u4e86\u6bcf\u4e2a\u7ec6\u80de\u7c07\u4e2d\u8868\u8fbe\u67d0\u4e2a\u6807\u8bb0\u57fa\u56e0\u7684\u7ec6\u80de\u7684\u767e\u5206\u6bd4\uff08\u70b9\u7684\u5927\u5c0f\uff09\u548c\u8be5\u7ec6\u80de\u7c07\u7684\u5e73\u5747\u68c0\u6d4b\u5230\u7684\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u3002\u70ed\u56fe\u663e\u793a\u4e86\u4e0d\u540c\u7ec6\u80de\u7c07\u7684\u5e73\u5747\u6807\u8bb0\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u3002\u8fd9\u4e24\u79cd\u56fe\u90fd\u662f\u7528Seurat\u521b\u5efa\u7684\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">require(dplyr)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u68c0\u7d22\u6bcf\u4e2a\u7c07\u7684\u524d 5 \u4e2a\u6807\u8bb0\u57fa\u56e0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u4f7f\u7528 AVG_LOG \u5217\u4e0b\u5177\u6709\u6700\u9ad8\u503c\u7684\u57fa\u56e0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">top5 <- markers_seur %>% group_by(cluster) %>%<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> dplyr::slice_max(get(grep(\"^avg_log\", colnames(markers_seur), value = TRUE)),<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> n = 5)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u7ed8\u5236\u70b9\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">Seurat::DotPlot(query_seur, features = unique(top5$gene)) +<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> ggplot2::theme(axis.text.x = ggplot2::element_text(angle = 90, vjust = 1, <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> size = 8, hjust = 1)) +<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> Seurat::NoLegend()<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/section><\/pre>\n<\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(0, 112, 192);font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><o:p><\/o:p><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015560\" data-ratio=\"0.6796296296296296\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/17_icxRomyEbDPtnjT0MX4ynpib1sDTBg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP6ic1FP9icgfWrx938ibnpMKtp6PfEnicxRomyEbDPtnjT0MX4ynpib1sDTBg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"376.07000732421875\" style=\"letter-spacing: 0.578px;white-space: normal;\" width=\"553.739990234375\" \/>    \u5982\u56fe\u6240\u793a\uff0c\u6e05\u6670\u7684\u663e\u793a\u4e86\u6bcf\u4e2a\u7ec6\u80de\u7c07\u4e2d\u8868\u8fbe\u67d0\u4e2a\u6807\u8bb0\u57fa\u56e0\u7684\u7ec6\u80de\u7684\u767e\u5206\u6bd4\uff08\u70b9\u7684\u5927\u5c0f\uff09\u548c\u8be5\u7ec6\u80de\u7c07\u7684\u5e73\u5747\u68c0\u6d4b\u5230\u7684\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u3002<\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"># \u7ed8\u5236\u70ed\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">Seurat::DoHeatmap(query_seur, features = unique(top5$gene)) +<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> Seurat::NoLegend() +<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> ggplot2::theme(axis.text.y = ggplot2::element_text(size = 8))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/section><\/pre>\n<\/section>\n<p><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015559\" data-ratio=\"0.7037037037037037\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/18_mUGIcLfnlh8lf83JtXw0rXohN30MLg.png?resize=553%2C389\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAy0GywOg1XfhBuHGjOqZbP6u0TBiccgGQyWFoVODyC6iaBiaawmUGIcLfnlh8lf83JtXw0rXohN30MLg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"389 .6700439453125\" style=\"letter-spacing: 0.578px;white-space: normal;\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"letter-spacing: 1px;font-size: 16px;\">\u5982\u56fe\u6240\u793a\uff0c\u663e\u793a\u4e86\u4e0d\u540c\u7ec6\u80de\u7c07\u7684\u5e73\u5747\u6807\u8bb0\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u3002<\/span><span style=\"letter-spacing: 1px;font-size: 16px;color: rgb(0, 112, 192);\">         <\/span><\/span><o:p style=\"color: rgb(0, 112, 192);font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> <\/span><\/o:p><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;color: rgb(0, 112, 192);font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><o:p><\/o:p><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;\">\u901a\u8fc7\u4e0a\u8ff0\u7684\u5de5\u5177\u548c\u65b9\u6cd5\u53ef\u4ee5\u4e3ascRNA-seq\u6570\u636e\u63d0\u4f9b\u53ef\u9760\u7684\u7ec6\u80de\u7c7b\u578b\u6807\u8bb0\u3002\u7136\u800c\uff0c\u7531\u4e8e\u7b97\u6cd5\u7684\u6d6e\u52a8\u4ee5\u53ca\u6807\u8bb0\u7684\u6a21\u7cca\u6027\uff0c\u4f7f\u7528\u5176\u4ed6\u7684\u65b9\u6cd5(\u4f8b\u5982\u7edf\u8ba1\u65b9\u6cd5)\u6216\u8fdb\u884c\u5b9e\u9a8c\u6765\u786e\u8ba4\u5355\u5143\u683c\u6ce8\u91ca\u6807\u7b7e\u662f\u975e\u5e38\u91cd\u8981\u7684\u3002\u6b64\u5916\uff0c\u7531\u4e8emRNA\u6d4b\u91cf\u53ea\u80fd\u90e8\u5206\u5b9a\u4e49\u7ec6\u80de\u7c7b\u578b\u548c\u529f\u80fd\uff0c\u56e0\u6b64\u5173\u4e8e\u65b0\u7ec6\u80de\u7c7b\u578b\u7684\u91cd\u8981\u7ed3\u8bba\u5fc5\u987b\u7ecf\u8fc7\u5b9e\u9a8c\u9a8c\u8bc1\u3002<\/span><span style=\"font-size: 16px;letter-spacing: 1px;\">        <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 1px;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> <\/span><\/o:p><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><o:p><\/o:p><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;font-variant: normal;text-transform: none;\">\u4f8b\u5982\uff0c\u53ef\u4ee5\u4f7f\u7528T\u7ec6\u80de\u53d7\u4f53(TCR)\u548cB\u7ec6\u80de\u53d7\u4f53\u514b\u9686\u5206\u578b\u6765\u6539\u8fdb\u7ec4\u7ec7\u9a7b\u7559\u514d\u75ab\u7ec6\u80de\u7684\u7ec6\u80de\u7c7b\u578b\u6807\u8bb0\uff0c\u4ee5\u68c0\u67e5T\u7ec6\u80de\u548cB\u7ec6\u80de\u7684\u8f6c\u5f55\u7279\u5f81\uff0c\u5206\u522b\u7531\u5b83\u4eec\u8868\u8fbe\u7684TCR\u548cB\u7ec6\u80de\u53d7\u4f53\u5206\u5c42\u3002<\/span>    <\/span><span style=\"font-size: 16px;letter-spacing: 1px;\">         <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 1px;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"> <\/span><\/o:p><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><o:p><\/o:p><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"letter-spacing: 1px;font-size: 16px;font-variant: normal;text-transform: none;\">\u4ee5\u4e0a\u5c31\u662f\u5c0f\u679c\u4eca\u5929\u7ed9\u5927\u5bb6\u5e26\u6765\u7684\u5168\u90e8\u5185\u5bb9\u5566\uff01\u5355\u7ec6\u80de\u6ce8\u91ca\u662f\u5355\u7ec6\u80de\u7684\u8f6c\u5f55\u7ec4\u5206\u6790\u4e2d\u975e\u5e38\u91cd\u8981\u7684\u4e00\u6b65\uff0c\u65e0\u8bba\u662f\u81ea\u52a8\u6ce8\u91ca\u8fd8\u662f\u624b\u52a8\u9009\u62e9\u6807\u8bb0\u57fa\u56e0\u8fdb\u884c\u6ce8\u91ca\uff0c\u90fd\u5177\u6709\u4e00\u5b9a\u7684\u4e0d\u786e\u5b9a\u6027\uff0c\u4f46\u662f\u6211\u4eec\u53ef\u4ee5\u901a\u8fc7\u5c06\u591a\u79cd\u81ea\u52a8\u6ce8\u91ca\u4ee5\u53ca\u624b\u52a8\u6ce8\u91ca\u7684\u7ed3\u679c\u8fdb\u884c\u6574\u5408\uff0c\u4f7f\u5f97\u81ea\u5df1\u5f97\u5206\u6790\u6570\u636e\u505a\u5230\u76f8\u5bf9\u7684\u7cbe\u51c6\u3002<\/span><span style=\"letter-spacing: 1px;font-size: 16px;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);\">\u5982\u679c\u5c0f\u4f19\u4f34\u4eec\u6709\u4efb\u4f55\u7591\u95ee\uff0c\u6b22\u8fce\u5173\u6ce8\u5c0f\u679c\uff0c\u5f53\u7136\uff0c\u4e5f\u53ef\u4ee5\u8bd5\u4e00\u8bd5\u6211\u4eec\u7684\u4e91\u751f\u4fe1\u5c0f\u5de5\u5177\uff0c\u53ea\u8981\u8f93\u5165\u5408\u9002\u7684\u6307\u4ee4\u5c31\u53ef\u4ee5\u76f4\u63a5\u7ed8\u5236\u60f3\u8981\u7684\u56fe\u5462\uff0c\u94fe\u63a5\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002<\/span><\/span><\/section>\n<section style=\"text-indent: 0px;text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><span style=\"color: rgb(0, 122, 170);font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong><span style=\"letter-spacing: 1px;font-size: 16px;color: rgb(0, 122, 170);text-indent: 32px;background-color: rgb(255, 255, 255);\">\u5c0f\u679c\u8fd8<span style=\"letter-spacing: 1px;font-size: 16px;color: rgb(0, 122, 170);background-color: rgb(255, 255, 255);text-decoration: none solid rgba(0, 0, 0, 0.9);\">\u63d0\u4f9b\u601d\u8def\u8bbe\u8ba1\u3001\u5b9a\u5236\u751f\u4fe1\u5206\u6790\u3001\u6587\u732e\u601d\u8def\u590d\u73b0\uff1b\u6709\u9700\u8981\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u76f4\u63a5\u626b\u7801\u54a8\u8be2\u5c0f\u679c\uff0c\u7aed\u8bda\u4e3a\u60a8\u7684\u79d1\u7814\u52a9\u529b\uff01<\/span><\/span><\/strong><\/span><\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\"><br \/><\/section>\n<section style=\"font-size: 15px;padding-right: 2px;padding-left: 2px;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\">\n<section style=\"text-align: justify;justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;flex: 0 0 auto;height: auto;border-color: rgb(114, 114, 114);border-width: 1px;border-style: solid;box-shadow: rgb(194, 209, 255) -5px 5px 0px;align-self: flex-start;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 42.6829%;vertical-align: middle;box-shadow: rgb(43, 65, 110) 0px 0px 0px;flex: 0 0 auto;height: auto;padding-left: 10px;align-self: center;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;line-height: 0;transform: translate3d(20px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 75%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015435\" data-ratio=\"1\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/19_B2jkicacLzPjw4xMSqVvoucZjonVDA.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxVYfSsM0wdf9d3RpNiaTcHohxPNEVs2VFhPMukpU8lchxrvcB2jkicacLzPjw4xMSqVvoucZjonVDA\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"430\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 56.9106%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"line-height: 2;letter-spacing: 1px;color: rgb(43, 65, 110);\" powered-by=\"xiumi.us\">\n<p style=\"margin-bottom: 2px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>\u5b9a\u5236\u751f\u4fe1\u5206\u6790<\/strong><\/span><\/p>\n<p style=\"margin-bottom: 2px;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>\u670d\u52a1\u5668\u79df\u8d41<\/strong><\/span><\/p>\n<p><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>\u626b\u7801\u54a8\u8be2\u5c0f\u679c<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497780&idx=2&sn=7eee90af6f387e4153bba13028e545a1&chksm=cf7f68f1f808e1e7dfb3a0597ef442e5c6e700ecf6ee5a035c766a4c7486c12eabd0ab218ea6&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015437\" data-ratio=\"0.3253333333333333\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-01-25_\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01\/20_7TvAyUibd1TPhJmZfialJtv6tu2SEg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxVYfSsM0wdf9d3RpNiaTcHoj9xlsoyiaBvzaezDUFAicibMAF8ts7TvAyUibd1TPhJmZfialJtv6tu2SEg\/640?wx_fmt=png&from=appmsg'\" data-type=\"png\" data-w=\"750\" style=\"vertical-align: middle;width: 100%;\" \/><\/span><\/span><\/a><\/section>\n<\/section>\n<section style=\"text-align: justify;line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img js_insertlocalimg\" data-imgfileid=\"100015436\" data-ratio=\"0.3811111111111111\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/21_9N02aBaau07RufXeXRLmvAHsrFZxtw.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAz5wg6Ne6sYkannSE9NiatkcPh3icbKanJagacjU6LqJrGAL29N02aBaau07RufXeXRLmvAHsrFZxtw\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"900\" style=\"letter-spacing: 0.578px;text-align: center;text-wrap: wrap;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\">\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;text-align: justify;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-end;padding-right: 2px;padding-left: 2px;\">\n<section style=\"margin: 10px 0% 2px;transform: translate3d(2px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 16px;color: rgb(58, 66, 94);line-height: 1;letter-spacing: 5px;text-align: center;\">\n<p><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>\u5f80\u671f\u56de\u987e<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"height: 3px;background-color: rgb(95, 156, 239);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: 2px;margin-right: 0%;margin-left: 0%;text-align: center;line-height: 0.8;font-size: 9px;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;border-top: 0.8em solid rgb(58, 66, 94);border-left: 0.7em solid transparent !important;border-right: 0.7em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;flex: 100 100 0%;align-self: flex-end;height: auto;\">\n<section style=\"margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"height: 3px;background-color: rgb(95, 156, 239);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: 2px;margin-right: 0%;margin-left: 0%;text-align: center;line-height: 0.8;font-size: 9px;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;border-top: 0.8em solid rgba(255, 255, 255, 0);border-left: 0.7em solid transparent !important;border-right: 0.7em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"min-height: 40px;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"width: 100%;margin-right: auto;margin-bottom: -10px;margin-left: auto;\">\n<table width=\"100%\">\n<tbody>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:0\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:0.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\">\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;text-align: justify;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;box-shadow: rgb(0, 0, 0) 0px 0px 0px;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>01<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497780&idx=2&sn=7eee90af6f387e4153bba13028e545a1&chksm=cf7f68f1f808e1e7dfb3a0597ef442e5c6e700ecf6ee5a035c766a4c7486c12eabd0ab218ea6&scene=21#wechat_redirect\" textvalue=\"1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff0c\u4e24\u4eba\u6210\u56e2\uff0c1\u4eba\u514d\u5355\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff0c\u4e24\u4eba\u6210\u56e2\uff0c1\u4eba\u514d\u5355\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:1\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:1.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\">\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;text-align: justify;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>02<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498878&idx=1&sn=fc34ed3f1d15d060bbaf8166adcebd58&chksm=cf7f6cbbf808e5ad0b5417e50ed75cfbd1b3a83ac43b97f1dfa718c822878a9a004990075133&scene=21#wechat_redirect\" textvalue=\"\u5355\u4e2a\u6570\u636e\u5e93\u7528\u817b\u4e86\uff1f\u591a\u6570\u636e\u5e93\u201c\u7ec4\u5408\u62f3\u201d\u5e26\u4f60\u6253\u5f00\u514d\u75ab\u6d78\u6da6\u65b0\u601d\u8def\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u5355\u4e2a\u6570\u636e\u5e93\u7528\u817b\u4e86\uff1f\u591a\u6570\u636e\u5e93\u201c\u7ec4\u5408\u62f3\u201d\u5e26\u4f60\u6253\u5f00\u514d\u75ab\u6d78\u6da6\u65b0\u601d\u8def\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:2\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:2.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\">\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;text-align: justify;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>03<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498673&idx=1&sn=1e09b74a836c1e64a62ae1f66eeb3f93&chksm=cf7f6b74f808e262de70be43fd73bf5fefdc0914e525eb2032f741a0152e619689cedc76946f&scene=21#wechat_redirect\" textvalue=\"\u5b5f\u5fb7\u5c14\u968f\u673a\u5316\u7684\u51c6\u5907\u5de5\u4f5c\uff0cGWAS\u6570\u636e\u7684\u7f51\u7ad9\u4e0b\u8f7d\u65b9\u6cd5\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"11\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u5b5f\u5fb7\u5c14\u968f\u673a\u5316\u7684\u51c6\u5907\u5de5\u4f5c\uff0cGWAS\u6570\u636e\u7684\u7f51\u7ad9\u4e0b\u8f7d\u65b9\u6cd5<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:3\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:3.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section style=\"line-height: 1.6em;margin-top: 8px;margin-bottom: 8px;\">\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;text-align: justify;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>04<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);font-size: 16px;letter-spacing: 1px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498517&idx=1&sn=fa3c0104cee316982fe8162b79fe234f&chksm=cf7f6bd0f808e2c6cf979704bf883cfb5480444604f90da61dc286e7541fa5bb49df0a2fb58a&scene=21#wechat_redirect\" textvalue=\"\u8ddf\u7740\u5c0f\u679c\u5b66\u590d\u73b0-\u624b\u628a\u624b\u5e26\u4f60\u62ff\u4e0bIF=46.9Nature \u7ea7\u522b\u7684\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u56fe\uff01\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u8ddf\u7740\u5c0f\u679c\u5b66\u590d\u73b0-\u624b\u628a\u624b\u5e26\u4f60\u62ff\u4e0bIF=46.9Nature \u7ea7\u522b\u7684\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u56fe\uff01\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u60f3\u63d0\u9ad8\u5355\u7ec6\u80de\u6ce8\u91ca\u51c6\u786e\u5ea6\uff1f\u201c\u624b\u52a8\u6821\u6b63+\u9a8c\u8bc1\u5206\u6790\u201d\u5fc5\u4e0d\u53ef\u5c11\uff01 \u5c0f\u679c  \u751f\u4fe1\u679c &nb […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58194"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=58194"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58194\/revisions"}],"predecessor-version":[{"id":58207,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58194\/revisions\/58207"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=58194"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=58194"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=58194"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}