{"id":58291,"date":"2024-06-16T22:41:08","date_gmt":"2024-06-16T14:41:08","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=58291"},"modified":"2024-06-16T22:41:08","modified_gmt":"2024-06-16T14:41:08","slug":"%e5%8d%95%e7%bb%86%e8%83%9e%e8%a1%a8%e5%9e%8b%e9%a2%84%e6%b5%8b%e9%bb%91%e7%a7%91%e6%8a%80%ef%bc%813%e5%88%86%e9%92%9f%e5%b8%a6%e4%bd%a0%e8%a7%a3%e9%94%81scpp%e5%ae%8c%e5%85%a8%e4%bd%93%ef%bc%81-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=58291","title":{"rendered":"\u5355\u7ec6\u80de\u8868\u578b\u9884\u6d4b\u9ed1\u79d1\u6280\uff013\u5206\u949f\u5e26\u4f60\u89e3\u9501ScPP\u5b8c\u5168\u4f53\uff01\uff01"},"content":{"rendered":"


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rel=\"noopener\">#\u751f\u4fe1\u5c0f\u767d\u76f8\u5173<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2812010014583390210#wechat_redirect\" title=\"R\u4ee3\u7801\" target=\"_blank\" rel=\"noopener\">#R\u4ee3\u7801<\/a><\/p>\n<\/div>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\">  <\/span><o:p><span style=\"font-size: 16px;\"> <\/span><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015147\" data-ratio=\"0.23333333333333334\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_rE2v0O5WiaUDJjgv5bxK2G1ePHyH2g-57.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAysHY49vPGCraibakiaNq6hQgn8C5MfvVLQGxlNZPEia2PajKAzrE2v0O5WiaUDJjgv5bxK2G1ePHyH2g\/640?wx_fmt=jpeg&wxfrom=5&wx_lazy=1&wx_co=1'\" data-w=\"1080\" data-recalc-dims=\"1\" \/><\/o:p><br \/><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u5355\u7ec6\u80de\u5206\u6790\u4e00\u76f4\u662f\u751f\u4fe1\u4ee5\u53ca\u533b\u5b66\u671f\u520a\u6bd4\u8f83\u6d41\u884c\u7684\u65b9\u5411\uff0c\u5c0f\u679c\u5728\u6d4f\u89c8\u671f\u520a\u7684\u65f6\u5019\u53d1\u73b0\u4e86\u8fd9\u6837\u4e00\u4e2aR\u5305\u2014ScPP\uff08Single Cells\u2019Phenotype 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style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u6211\u4eec\u5728\u8fdb\u884c\u5355\u7ec6\u80de\u8868\u578b\u9884\u6d4b\u65f6\u901a\u5e38\u9700\u8981\u4ee5\u4e0b\u51e0\u4e2a\u6b65\u9aa4\uff1a<\/span><span style=\"font-family: \u7b49\u7ebf;letter-spacing: 0.034em;\">         <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><span style=\"font-size: 16px;\"> <\/span><\/o:p><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">1.\u4ece\u5355\u7ec6\u80de\u6570\u636e\u4e2d\u63d0\u53d6\u7279\u5f81\uff0c\u6bd4\u5982\u8bf4\u57fa\u56e0\u8868\u8fbe\u3001\u8868\u89c2\u9057\u4f20\u4fee\u9970\u3001\u86cb\u767d\u8d28\u6c34\u5e73\u6216\u7ec6\u80de\u5f62\u6001\u7b49\u7279\u5f81\u3002<\/span><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">2.\u9009\u62e9\u4e0e\u8868\u578b\u76f8\u5173\u7684\u7279\u5f81\uff0c\u53ef\u4ee5\u901a\u8fc7\u5dee\u5f02\u5206\u6790\u3001\u7279\u5f81\u9009\u62e9\u6216\u6df1\u5ea6\u5b66\u4e60\u7b49\u65b9\u6cd5\u8fdb\u884c\u7b5b\u9009\u3002<\/span><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">3.\u5efa\u7acb\u9884\u6d4b\u6a21\u578b\uff0c\u8fd9\u4e00\u6b65\u53ef\u4ee5\u901a\u8fc7\u76d1\u7763\u5b66\u4e60\u3001\u65e0\u76d1\u7763\u5b66\u4e60\u6216\u534a\u76d1\u7763\u5b66\u4e60\u7b49\u65b9\u6cd5\u5b8c\u6210\u3002<\/span><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">4.\u9a8c\u8bc1\u548c\u8bc4\u4f30\u9884\u6d4b\u7ed3\u679c\uff0c\u901a\u8fc7\u4ea4\u53c9\u9a8c\u8bc1\u3001ROC\u66f2\u7ebf\u3001\u6df7\u6dc6\u77e9\u9635\u6216\u751f\u7269\u5b66\u5b9e\u9a8c\u7b49\u65b9\u6cd5\u8fdb\u884c\u9a8c\u8bc1\u3002<\/span>    <span style=\"font-family: \u7b49\u7ebf;letter-spacing: 0.034em;\">         <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><span style=\"font-size: 16px;\"> <\/span><\/o:p><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u4e86\u89e3\u4e86\u4ec0\u4e48\u662f\u5355\u7ec6\u80de\u8868\u578b\u9884\u6d4b\uff0c\u6211\u4eec\u6765\u770b\u4e00\u4e0b\u4eca\u5929\u7684\u4e3b\u89d2\u2014\u2014ScPP\u3002\u4e0b\u9762\u7684\u56fe\u7247\u662f\u901a\u8fc7ScPP\u8fdb\u884c\u5355\u7ec6\u80de\u8868\u578b\u9884\u6d4b\u7684\u6d41\u7a0b\u56fe\uff1a<\/span><span style=\"letter-spacing: 0.034em;\">        <\/span><\/span><\/section>\n<table style=\"width: 100%;\">\n<tbody>\n<tr>\n<td width=\"0.0\" height=\"28.267097\" style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;\"><br \/><\/td>\n<td width=\"0.0\" height=\"28.267097\" style=\"border-width: initial;border-style: none;border-color: initial;\"><br \/><\/td>\n<\/tr>\n<tr>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\"><br \/><\/td>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\">\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100015142\" data-ratio=\"0.8089887640449438\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_5PG28xtd9uOgxShPDT0CMtMtepBUSw.png?resize=488%2C395\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPOe9tUFY5Qb6zia1UHx0JvgHAeUfq1Z1ChI2icWbR3z4anI5PG28xtd9uOgxShPDT0CMtMtepBUSw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1068\" height=\"395 .010009765625\" width=\"488 .6700439453125\" data-recalc-dims=\"1\" \/><span style=\"font-size: 16px;\"><br \/>\n        <\/span><\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><br \/><\/p>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><br \/><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u9996\u5148\uff0c\u6211\u4eec\u53ef\u4ee5\u901a\u8fc7Bulk RNA-seq\u83b7\u53d6\u4e00\u7ec4\u7ec6\u80de\u7684\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u6570\u636e\uff0c\u7136\u540e\u8fd0\u7528Binary\u8fdb\u884c\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u5f53\u7136\u4e5f\u53ef\u4ee5\u4f7f\u7528\u751f\u5b58\u5206\u6790\u6216\u8005\u8fde\u7eed\u53d8\u91cf\u76f8\u5173\u6027\u5206\u6790\uff0c\u63d0\u53d6\u51faPhenotype+ \u6807\u8bb0\u57fa\u56e0\u4ee5\u53caPhenotype-\u6807\u8bb0\u57fa\u56e0\uff0c\u8fdb\u800c\u5c06\u5176\u7528\u4e8e\u9274\u5b9a\u548c\u5206\u7c7b\u7ec6\u80de\u3002<\/span><span style=\"font-family: \u7b49\u7ebf;letter-spacing: 0.034em;\">        <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><span style=\"font-size: 16px;\"> <\/span><\/o:p><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u9664\u4e86Bulk RNA-seq\uff0c\u4e5f\u53ef\u4ee5\u76f4\u63a5\u8fdb\u884cSingle-cell RNA-seq\uff0c\u627e\u5230Phenotype\u6807\u8bb0\u57fa\u56e0\uff0c\u7136\u540e\u8fdb\u884cAUCell\u5206\u6790\uff0c\u8bc6\u522b\u5177\u6709\u6807\u8bb0\u57fa\u56e0\u7684\u7ec6\u80de\u7fa4\uff0c\u5e76\u4e14\u5c06Phenotype-\u4ee5\u53caPhenotype+\u533a\u5206\u7684\u7ed3\u679c\u8fdb\u884c\u5408\u5e76\uff0c\u5c31\u53ef\u4ee5\u5f97\u5230\u5728\u6574\u4e2a\u7ec6\u80de\u4e2d\uff0c\u8868\u578b\u57fa\u56e0\u8868\u8fbe\u9ad8\u548c\u8868\u8fbe\u4f4e\u7684\u5206\u5e03\u56fe\uff0c\u4e5f\u5c31\u5b8c\u6210\u4e86\u8868\u578b\u9884\u6d4b\u3002<\/span>    <span style=\"font-family: \u7b49\u7ebf;letter-spacing: 0.034em;\">         <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><span style=\"font-size: 16px;\"> <\/span><\/o:p><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u63a5\u4e0b\u6765\u662f\u4ee3\u7801\u5b9e\u64cd\uff1a<\/span><span style=\"font-family: \u7b49\u7ebf;letter-spacing: 0.034em;\">         <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><span style=\"font-size: 16px;\"> <\/span><\/o:p><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">Step 1 \u8fdb\u884cScPP\u5305\u7684\u521d\u59cb\u5316<\/span><span style=\"font-family: \u7b49\u7ebf;letter-spacing: 0.034em;\">         <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><span style=\"font-size: 16px;\"> <\/span><\/o:p><\/section>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">#\u5bfc\u5165ScPP\u4e2d\u7684\u529f\u80fd\u51fd\u6570<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">##\u4e3a\u5355\u7ec6\u80de\u8868\u8fbe\u6570\u636e\u8fdb\u884c\u9884\u5904\u7406\u3002\u8f93\u5165\u6570\u636e\u662f\u4e00\u4e2a\u8ba1\u6570\u77e9\u9635\uff0c\u5176\u4e2d\u7ec6\u80de\u662f\u5217\u540d\uff0c\u57fa\u56e0\u662f\u884c\u540d<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">sc_Preprocess <- function(counts, project = \"sc_preprocess\",<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> normalization.method = \"LogNormalize\", scale.factor = 10000,<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> selection.method = \"vst\", resolution = 0.1,<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> dims_Neighbors = 1:20, dims_TSNE = 1:20, dims_UMAP = 1:20){<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> library(Seurat)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> sc_dat <- CreateSeuratObject(counts = counts, project = project)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> sc_dat <- NormalizeData(object = sc_dat, normalization.method = normalization.method, scale.factor = scale.factor)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> sc_dat <- FindVariableFeatures(object = sc_dat, selection.method = selection.method)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> sc_dat <- ScaleData(object = sc_dat, features = rownames(sc_dat)) <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> sc_dat <- RunPCA(object = sc_dat, features = VariableFeatures(sc_dat))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> sc_dat <- FindNeighbors(object = sc_dat, dims = dims_Neighbors)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> sc_dat <- FindClusters(object = sc_dat, resolution = resolution)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> sc_dat <- RunTSNE(object = sc_dat, dims = dims_TSNE)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> sc_dat <- RunUMAP(object = sc_dat, dims = dims_UMAP)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">return(sc_dat)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">}<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">#\u8fd0\u7528Binary\u8fdb\u884c\u5dee\u5f02\u8868\u8fbe\u5206\u6790<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">##\u751f\u6210\u4e8c\u5143\u53d8\u91cf\u4e0e\u8868\u578b+\u6216\u8868\u578b-\u7ec4\u76f8\u5173\u7684\u6807\u8bb0\u57fa\u56e0\u4ee5\u53ca\u7279\u5f81\uff0c\u4e00\u5171\u5305\u542b4\u4e2a\u53c2\u6570:bulk_data\u3001features\u3001ref_group\u548cLog2FC_cutoff<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">marker_Binary <- function(bulk_data, features, ref_group, Log2FC_cutoff = 0.585){<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> library(dplyr)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if (missing(ref_group))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> stop(\"'ref_group' is missing or incorrect.\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if (missing(bulk_data) || !class(bulk_data) %in% c(\"matrix\", \"data.frame\"))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> stop(\"'bulk_data' is missing or incorrect.\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if (missing(features) || !class(features) %in% c(\"matrix\", \"data.frame\"))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> stop(\"'features' is missing or incorrect.\") <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">ref = features$Sample[features$Feature == ref_group]<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> tes = features$Sample[features$Feature != ref_group]<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> ref_pos = which(colnames(bulk_data) %in% ref)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> tes_pos = which(colnames(bulk_data) %in% tes)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> pvalues <- apply(bulk_data, 1, function(x) {<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> t.test(as.numeric(x)[tes_pos], as.numeric(x)[ref_pos])$p.value<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> })<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> log2FCs <- rowMeans(bulk_data[, tes_pos]) - rowMeans(bulk_data[, ref_pos])<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> res <- data.frame(pvalue = pvalues, log2FC = log2FCs)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> res <- res[order(res$pvalue), ]<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> res$fdr <- p.adjust(res$pvalue, method = \"fdr\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> geneList <- list(<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> gene_pos = res %>% filter(pvalue < 0.05, log2FC > Log2FC_cutoff ) %>% rownames(.),<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> gene_neg = res %>% filter(pvalue < 0.05, log2FC < -Log2FC_cutoff ) %>% rownames(.)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> )<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if(length(geneList[[1]]) > 0 & length(geneList[[2]] > 0)){return(geneList)}<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">else if (length(geneList[[1]]) == 0){warning(\"There is no genes positively correlated with the given feature in this bulk dataset.\");geneList = list(gene_pos = geneList[[2]]);return(geneList)}<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">else if (length(geneList[[2]]) == 0){warning(\"There is no genes negatively correlated with the given feature in this bulk dataset.\");geneList = list(gene_neg = geneList[[1]]);return(geneList)}<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">}<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">#\u8fdb\u884c\u8fde\u7eed\u53d8\u91cf\u76f8\u5173\u6027\u5206\u6790<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">##\u751f\u6210\u8fde\u7eed\u53d8\u91cf\u4ee5\u53ca\u548c\u8868\u578b\u76f8\u5173\u7684\u6807\u8bb0\u57fa\u56e0\u6216\u7279\u5f81\uff0c\u5305\u542b4\u4e2a\u53c2\u6570:bulk_data\u3001features\u3001method\u548cestimate_cutoff<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">marker_Continuous <- function(bulk_data, features, method = \"spearman\", estimate_cutoff = 0.2){<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> library(dplyr)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if (missing(bulk_data) || !class(bulk_data) %in% c(\"matrix\", \"data.frame\"))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> stop(\"'bulk_data' is missing or incorrect.\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if (missing(features) || !class(features) %in% c(\"integer\", \"numeric\"))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> stop(\"'features' is missing or incorrect.\") <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> CorrelationTest = apply(bulk_data,1,function(x){<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> pvalue = cor.test(as.numeric(x),log2(as.numeric(features)+1), method = method)$p.value <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> estimate = cor.test(as.numeric(x),log2(as.numeric(features)+1), method = method)$estimate<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> res = cbind(pvalue,estimate)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">return(res)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> })<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> resc = t(CorrelationTest)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> colnames(resc) = c(\"pvalue\",\"estimate\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> resc = as.data.frame(resc[order(as.numeric(resc[,1]),decreasing = FALSE),])<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> resc$fdr <- p.adjust(resc$pvalue,method = \"fdr\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> geneList <- list(<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> gene_pos = resc %>% filter(fdr < 0.05, estimate > estimate_cutoff) %>% rownames(.),<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> gene_neg = resc %>% filter(fdr < 0.05, estimate < -estimate_cutoff) %>% rownames(.)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> )<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if(length(geneList[[1]]) > 0 & length(geneList[[2]] > 0)){return(geneList)}<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">else if (length(geneList[[1]]) == 0){warning(\"There is no genes positively correlated with the given feature in this bulk dataset.\");geneList = list(gene_pos = geneList[[2]]);return(geneList)} <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">else if (length(geneList[[2]]) == 0){warning(\"There is no genes negatively correlated with the given feature in this bulk dataset.\");geneList = list(gene_neg = geneList[[1]]);return(geneList)}<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">}<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">##\u751f\u5b58\u6570\u636e\u5206\u6790\uff0c\u751f\u6210\u4e0e\u60a3\u8005\u9884\u540e\u76f8\u5173\u7684\u6807\u8bb0\u57fa\u56e0\u6216\u7279\u5f81\uff0c\u5305\u542b2\u4e2a\u53c2\u6570:bulk_data\u548csurvival_data<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">marker_Survival <- function(bulk_data,survival_data){<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> library(survival)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> library(dplyr)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if (missing(bulk_data) || !class(bulk_data) %in% c(\"matrix\", \"data.frame\"))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> stop(\"'bulk_data' is missing or incorrect.\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if (missing(survival_data) || !class(survival_data) %in% c(\"matrix\", \"data.frame\"))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> stop(\"'survival_data' is missing or incorrect.\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> SurvivalData <- data.frame(cbind(survival_data,t(bulk_data)))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> colnames(SurvivalData) = make.names(colnames(SurvivalData))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> var <- make.names(rownames(bulk_data))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> Model_Formula <- sapply(var, function(x) as.formula(paste(\"Surv(time, status) ~\", x))) <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> Model_all <- lapply(Model_Formula, function(x) coxph(x, data = SurvivalData))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> res <- lapply(seq_along(Model_all), function(i) {<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> coef_summary <- summary(Model_all[[i]])$coefficients<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> data.frame(<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> variable = var[i],<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> pvalue = coef_summary[,5],<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> coef = coef_summary[,2]<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> )<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> }) %>% bind_rows()<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> res <- res[order(res$pvalue), ]<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> res$fdr <- p.adjust(res$pvalue, method = \"fdr\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> geneList <- list(<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> gene_pos = res %>% filter(fdr < 0.05, coef > 1) %>% pull(variable), #correalted with worse survival<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> gene_neg = res %>% filter(fdr < 0.05, coef < 1) %>% pull(variable) #correlated with better survival<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> )<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if(length(geneList[[1]]) > 0 & length(geneList[[2]] > 0)){return(geneList)}<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">else if (length(geneList[[1]]) == 0){warning(\"There is no genes negatively correlated with patients' prognosis in this bulk dataset.\");geneList = list(gene_pos = geneList[[2]]);return(geneList)} <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">else if (length(geneList[[2]]) == 0){warning(\"There is no genes positively correlated with patients' prognosis in this bulk dataset.\");geneList = list(gene_neg = geneList[[1]]);return(geneList)}<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">}<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">##\u8fdb\u884c\u5355\u7ec6\u80de\u8868\u578b\u9884\u6d4b\uff0c\u5305\u542b3\u4e2a\u53c2\u6570:sc_dataset, geneList\u548cprobs\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">ScPP = function(sc_dataset, geneList, probs = 0.2){<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if(length(geneList) != 2){<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> stop(\"This gene list do not have enough information correlated with interested feature.\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> }<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if (missing(sc_dataset) || class(sc_dataset) != \"Seurat\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> stop(\"'sc_dataset' is missing or not a seurat object.\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> library(AUCell)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> cellrankings = AUCell_buildRankings(sc_dataset@assays$RNA@data,plotStats = FALSE)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> cellAUC = AUCell_calcAUC(geneList,cellrankings)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> metadata = as.data.frame(sc_dataset@meta.data)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> metadata$AUCup <- as.numeric(getAUC(cellAUC)[\"gene_pos\", ]) <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> metadata$AUCdown <- as.numeric(getAUC(cellAUC)[\"gene_neg\", ])<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> downcells1 = rownames(metadata)[which(metadata$AUCup <= quantile(metadata$AUCup,probs = probs))]<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> upcells1 = rownames(metadata)[which(metadata$AUCup >= quantile(metadata$AUCup,probs = (1-probs)))]<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> downcells2 = rownames(metadata)[which(metadata$AUCdown >= quantile(metadata$AUCdown,probs = (1-probs)))]<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> upcells2 = rownames(metadata)[which(metadata$AUCdown <= quantile(metadata$AUCdown,probs = probs))]<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> ScPP_neg = intersect(downcells1,downcells2)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> ScPP_pos = intersect(upcells1,upcells2)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> metadata$ScPP <- ifelse(rownames(metadata) %in% ScPP_pos, \"Phenotype+\", \"Background\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> metadata$ScPP <- ifelse(rownames(metadata) %in% ScPP_neg, \"Phenotype-\", metadata$ScPP)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">return(metadata)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">}<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">#\u5bfc\u5165\u7ed8\u56fe\u6240\u9700\u7684\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">library(ggplot2)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">#\u5b89\u88c5Scpp\u5305\u4ee5\u53ca\u76f8\u5173\u7684R\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if (!require(\"BiocManager\", quietly = TRUE))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">install.packages(\"BiocManager\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">BiocManager::install(\"AUCell\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">if (!requireNamespace(\"devtools\", quietly = TRUE))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">install.packages(\"devtools\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">devtools::install_github(\"WangX-Lab\/ScPP\")<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><br \/><\/p>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">Step 2 \u5e94\u7528\u4e8c\u5143\u53d8\u91cf\u8fdb\u884cScPP<\/span><span style=\"font-family: \u7b49\u7ebf;letter-spacing: 0.034em;\">       <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><span style=\"font-size: 16px;\"> <\/span><\/o:p><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">\u672c\u6b21\u5206\u6790\u6240\u9700\u7684\u6570\u636e\u5c0f\u679c\u5df2\u7ecf\u5e2e\u5927\u5bb6\u6574\u7406\u597d\u4e86\uff0c\u5927\u5bb6\u70b9\u51fb\u94fe\u63a5\u5373\u53ef\u4e0b\u8f7d\uff1a<\/span><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">\u94fe\u63a5\uff1ahttps:\/\/pan.baidu.com\/s\/1Kuox0OuispGobwKDeQJ0Bw<\/span><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u63d0\u53d6\u7801\uff1av4fz<\/span><span style=\"font-family: \u7b49\u7ebf;letter-spacing: 0.034em;\">        <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><span style=\"font-size: 16px;\"> <\/span><\/o:p><\/section>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"># \u51c6\u5907\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">load(\"\/data\/binary.RData\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"># \u6570\u636e\u603b\u89c8<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">bulk[1:6,1:6] # bulk data<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">head(binary) # phenotype data of bulk<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">sc_count[1:6,1:6] # scRNA-seq data<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"># \u901a\u8fc7 ScPP \u9009\u62e9\u4fe1\u606f\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">sc = sc_Preprocess(sc_count)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">geneList = marker_Binary(bulk, binary, ref_group = \"Normal\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">metadata = ScPP(sc, geneList)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">write.csv(metadata, \"\/results\/binary_meta.csv\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">head(metadata)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">sc$ScPP = metadata$ScPP<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">Idents(sc) = \"ScPP\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">#\u5bf9ScPP\u8bc6\u522b\u7684\u7ec6\u80de\u8fdb\u884c\u53ef\u89c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">DimPlot(sc, group = \"ScPP\", cols = c(\"grey\",\"blue\",\"red\"))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">ggsave(\"Fig_binary.pdf\",path = \"\/results\/\",width = 10, height = 10, units = \"cm\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"> <\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<p><br \/><\/p>\n<table style=\"width: 100%;\">\n<tbody>\n<tr>\n<td width=\"-13.333536\" height=\"23.067017\" style=\"border-width: initial;border-style: none;border-color: initial;\"><br \/><\/td>\n<td width=\"-13.333536\" height=\"23.067017\" style=\"border-width: initial;border-style: none;border-color: initial;\"><br \/><\/td>\n<\/tr>\n<tr>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\"><br \/><\/td>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\">\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015140\" data-ratio=\"0.434715821812596\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_8f10XbWKJb1WjiaNK3BmmLrzuaydXQ.png?resize=640%2C278\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPOe9tUFY5Qb6zia1UHx0JvXbbwJDEumQbsEMNqIxpLHicSbo8f10XbWKJb1WjiaNK3BmmLrzuaydXQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"651\" height=\"278 .27239990234375\" width=\"640 .0120239257812\" data-recalc-dims=\"1\" \/><span style=\"font-size: 16px;\"><br \/>\n        <\/span><\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\">          <\/span><\/section>\n<table style=\"width: 100%;\">\n<tbody>\n<tr>\n<td style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;\"><br \/><\/td>\n<td style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;\">\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100015139\" data-ratio=\"0.08383233532934131\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_iaoMBafAEic51yDGajVDrhuteUoKtQ.jpg?resize=500%2C41\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxPOe9tUFY5Qb6zia1UHx0Jv0MFda7DYJdyMfZAn9y56Q1iaQoMiaoMBafAEic51yDGajVDrhuteUoKtQ\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"501\" height=\"41 .60063552856445\" width=\"500 .67431640625\" data-recalc-dims=\"1\" \/><span style=\"font-size: 16px;\">      <\/span><\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><br \/><\/section>\n<p><br \/><\/p>\n<table style=\"width: 100%;\">\n<tbody>\n<tr>\n<td style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;\"><br \/><\/td>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\">\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015143\" data-ratio=\"0.47314814814814815\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_ZuibPUBkpuHfatniaVBDwx9jaq666g.png?resize=500%2C236\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPOe9tUFY5Qb6zia1UHx0JvpzL40uzTiaKg3Puq0TtGnaPTEA5ZuibPUBkpuHfatniaVBDwx9jaq666g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"236 .739990234375\" width=\"500 .6700439453125\" data-recalc-dims=\"1\" \/><span style=\"font-size: 16px;\"><br \/>\n        <\/span><\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><br \/><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">\u5bfc\u5165\u7684\u6570\u636e\u5982\u56fe\uff0c\u662f\u6211\u4eec\u8fdb\u884cBinary\u5206\u6790\u6240\u9700\u7684\u4e09\u4e2a\u6570\u636e\uff0c\u5206\u522b\u662f\uff1aBinary\u6570\u636e\uff08\u6837\u672c\u7684\u80bf\u7624\u4e0e\u6b63\u5e38\u7684\u4e8c\u5143\u6570\u636e\uff09\uff0cBulk\u6570\u636e\uff08\u8868\u578b\u6570\u636e\uff09\uff0csc_count\u6570\u636e\uff08scRNA\u7684\u6d4b\u5e8f\u6570\u636e\uff09\u3002<\/span><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><br \/><\/section>\n<table style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;width: 100%;\">\n<tbody>\n<tr>\n<td width=\"123.33521\" height=\"32.933834\" style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;\"><br \/><\/td>\n<td width=\"123.33521\" height=\"32.933834\" style=\"border-width: initial;border-style: none;border-color: initial;\"><br \/><\/td>\n<\/tr>\n<tr>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\"><br \/><\/td>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\">\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015141\" data-ratio=\"0.7472527472527473\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_uibKaZH6sg0kP74iaCXk2O3ZcWK4vw.png?resize=317%2C237\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPOe9tUFY5Qb6zia1UHx0Jvjhficb7DuAkgBkfCJaQBO36pPQGuibKaZH6sg0kP74iaCXk2O3ZcWK4vw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"728\" height=\"237 .33999633789062\" style=\"width: 269px;height: auto;\" width=\"317 .4000244140625\" data-recalc-dims=\"1\" \/><span style=\"font-size: 16px;\">         <\/span><\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><br \/><\/p>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><br \/><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">\u63a5\u7740\u662f\u6211\u4eec\u5206\u6790\u7684\u7ed3\u679c\uff0c\u901a\u8fc7ScPP\u5229\u7528\u4e8c\u5143\u53d8\u91cf\u5206\u6790\u5f97\u5230\u4e86\u7ec6\u80de\u4e2d\u7684\u80cc\u666f\u57fa\u56e0\uff0c\u4ee5\u53ca\u5e26\u6709\u6807\u8bb0\u57fa\u56e0\u7684\u9ad8\u8868\u8fbe\u548c\u4f4e\u8868\u8fbe\u7fa4\uff0c\u56fe\u4e2d\u7684\u7ea2\u8272\u8868\u793a\u9ad8\u8868\u8fbe\u7fa4\uff0c\u8bf4\u660e\u8fd9\u90e8\u5206\u7684\u7ec6\u80de\u53ef\u80fd\u662f\u5177\u6709\u6211\u4eec\u6240\u9700\u8981\u7684\u8868\u578b\u7684\u7ec6\u80de\u3002<\/span><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">          <o:p> <\/o:p><\/span><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">Step 3 \u8fd0\u7528\u8fde\u7eed\u53d8\u91cf\u8fdb\u884cScPP<\/span><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">          <o:p> <\/o:p><\/span><\/section>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"># \u5bfc\u5165\u6570\u636e\uff08\u6240\u9700\u6570\u636e\u5c0f\u679c\u653e\u5728\u4e0a\u6587\u7684\u94fe\u63a5\u4e86\u54e6~\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"># library(ScPP)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">load(\"\/data\/continuous.RData\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"># \u6570\u636e\u603b\u89c8<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">bulk[1:6,1:6] # bulk data<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">head(continuous) # phenotype data of bulk <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">sc_count[1:6,1:6] # scRNA-seq data<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"># \u901a\u8fc7 ScPP \u9009\u62e9\u4fe1\u606f\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">sc = sc_Preprocess(sc_count)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">geneList = marker_Continuous(bulk, continuous$TMB_non_silent)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">metadata = ScPP(sc, geneList)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">write.csv(metadata, \"\/results\/continuous_meta.csv\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">sc$ScPP = metadata$ScPP<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">Idents(sc) = \"ScPP\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">#\u5bf9ScPP\u8bc6\u522b\u7684\u7ec6\u80de\u8fdb\u884c\u53ef\u89c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">DimPlot(sc, group = \"ScPP\", cols = c(\"grey\",\"blue\",\"red\"))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">ggsave(\"Fig_continuous.pdf\",path = \"\/results\/\",width = 10, height = 10, units = \"cm\")<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><o:page><\/o:page><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\">          <\/span><\/section>\n<table style=\"width: 100%;\">\n<tbody>\n<tr>\n<td width=\"0.0\" height=\"27.33375\" style=\"border-width: initial;border-style: none;border-color: initial;\"><br \/><\/td>\n<td width=\"0.0\" height=\"27.33375\" style=\"border-width: initial;border-style: none;border-color: initial;\"><br \/><\/td>\n<\/tr>\n<tr>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\"><br \/><\/td>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\">\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015145\" data-ratio=\"0.7241379310344828\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_1I9cOXagslicGC4sGR2PPtr4ASEBuw.png?resize=442%2C320\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPOe9tUFY5Qb6zia1UHx0JvtLg2TNzZNiaWkKBgoU6eCIhFuic1I9cOXagslicGC4sGR2PPtr4ASEBuw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1015\" height=\"320 .20001220703125\" width=\"442 .26995849609375\" data-recalc-dims=\"1\" \/><span style=\"font-size: 16px;\"><br \/>\n        <\/span><\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><br \/><\/p>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><br \/><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">          <o:p> <\/o:p><\/span><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u7ed3\u679c\u7684\u53ef\u89c6\u5316\u5982\u56fe\uff0c\u53ef\u4ee5\u53d1\u73b0\u548c\u8fd0\u7528\u4e8c\u5143\u53d8\u91cf\u8fdb\u884c\u7684ScPP\u7684\u7ed3\u679c\u662f\u6bd4\u8f83\u76f8\u4f3c\u7684\uff0c\u8fd9\u4e5f\u53ef\u4ee5\u8bc1\u660eScPP\u8fd9\u4e2a\u5305\u7684\u5206\u6790\u6bd4\u8f83\u7a33\u5b9a\u3002ScPP\u7684\u5206\u6790\u6587\u4ef6\u50a8\u5b58\u5728csv\u6587\u4ef6\u4e2d\uff0c\u5c0f\u4f19\u4f34\u4eec\u4e5f\u53ef\u4ee5\u7528csv\u6587\u4ef6\u8fdb\u884c\u5176\u4ed6\u7684\u5206\u6790~<\/span><span style=\"letter-spacing: 0.034em;\">  <\/span><\/span><\/section>\n<table style=\"width: 100%;\">\n<tbody>\n<tr>\n<td width=\"18.66695\" height=\"25.600391\" style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;\"><br \/><\/td>\n<td width=\"18.66695\" height=\"25.600391\" style=\"border-width: initial;border-style: none;border-color: initial;\"><br \/><\/td>\n<\/tr>\n<tr>\n<td style=\"border-width: initial;border-style: none;border-color: initial;word-break: break-all;\"><br \/><\/td>\n<td style=\"border-width: initial;border-style: none;border-color: initial;\">\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015144\" data-ratio=\"0.5361111111111111\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_H6tlJHZ4r4WGUPHrwTlW5PGpaicwfA.png?resize=487%2C261\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPOe9tUFY5Qb6zia1UHx0JvVVUyYuNqLIs6gxP0JT0M1tAkvH6tlJHZ4r4WGUPHrwTlW5PGpaicwfA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"261 .47003173828125\" width=\"487 .34002685546875\" data-recalc-dims=\"1\" \/><span style=\"font-size: 16px;\"><br \/>\n        <\/span><\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"letter-spacing: 0.034em;font-family: \u7b49\u7ebf;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;font-size: 16px;\">   <o:p> <\/o:p><\/span><span style=\"letter-spacing: 0.034em;font-size: 16px;\">    <\/span><\/p>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><o:page><\/o:page><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">Step 4 \u8fd0\u7528\u751f\u5b58\u6570\u636e\u8fdb\u884cScPP<\/span><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">          <o:p> <\/o:p><\/span><span style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><\/span><\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">#\u5bfc\u5165\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">load(\"\/data\/survival.RData\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">#\u901a\u8fc7 ScPP \u9009\u62e9\u4fe1\u606f\u7ec6\u80de<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">sc = sc_Preprocess(sc_count)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">geneList = marker_Survival(bulk, survival)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">str(geneList)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">metadata = ScPP(sc, geneList)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">write.csv(metadata, \"\/results\/survival_meta.csv\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">sc$ScPP = metadata$ScPP<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">Idents(sc) = \"ScPP\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">#\u53ef\u89c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">DimPlot(sc, group = \"ScPP\", cols = c(\"grey\",\"blue\",\"red\"))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">ggsave(\"Fig_survival.pdf\",path = \"\/results\/\",width = 10, height = 10, units = \"cm\")<\/span><\/span><\/code><\/section><\/pre>\n<\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;color: rgb(0, 112, 192);font-size: 16px;\"><\/span><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;color: rgb(0, 112, 192);font-size: 16px;\">          <o:p> <\/o:p><\/span><span style=\"font-size: 16px;\">    <\/span><o:page><\/o:page><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015146\" data-ratio=\"0.7112195121951219\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_9nIiasPjNvpfqE0D9yPsZGxNU2qluA.png?resize=448%2C318\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPOe9tUFY5Qb6zia1UHx0JvkyMicXA23DFDqHqnkqIzYhSQFic9nIiasPjNvpfqE0D9yPsZGxNU2qluA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1025\" height=\"318 .739990234375\" width=\"448 .010009765625\" data-recalc-dims=\"1\" \/><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u7ed3\u679c\u5982\u56fe\uff0c\u53ef\u4ee5\u53d1\u73b0\u6211\u4eec\u8fdb\u884cScPP\u7684\u7ed3\u679c\u4e0e\u524d\u4e24\u6b21\u7684\u5206\u6790\u7565\u6709\u4e0d\u540c\uff0c\u4f46\u662f\u6574\u4f53\u7684\u7ec6\u80de\u8868\u578b\u5206\u5e03\u8fd8\u662f\u5927\u81f4\u76f8\u540c\u7684\u3002<\/span><span style=\"font-family: \u7b49\u7ebf;letter-spacing: 0.034em;\">       <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><span style=\"font-size: 16px;\"> <\/span><\/o:p><\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;\"><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;\">\u4ee5\u4e0a\u5c31\u662f\u5c0f\u679c\u4eca\u5929\u5206\u4eab\u7684\u5168\u90e8\u5185\u5bb9\u5566\uff01\u4eca\u5929\u6211\u4eec\u4e3b\u8981\u4e86\u89e3\u4e86\u4ec0\u4e48\u662f\u5355\u7ec6\u80de\u8868\u578b\u9884\u6d4b\uff0c\u4ee5\u53ca\u8fd0\u7528\u4e09\u79cd\u6570\u636e\uff08\u4e8c\u8fdb\u5236\u6570\u636e\uff0c\u751f\u5b58\u6570\u636e\uff0c\u8fde\u7eed\u6027\u6570\u636e\uff09\u5728ScPP\u8fd9\u4e2aR\u5305\u7684\u5e2e\u52a9\u4e0b\u8fdb\u884c\u5355\u7ec6\u80de\u8868\u578b\u9884\u6d4b\uff0c\u5e76\u4e14\u5bf9\u7ed3\u679c\u8fdb\u884c\u4e86\u89e3\u8bfb\u3002<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);\">\u5982\u679c\u5927\u5bb6\u5bf9\u4eca\u5929\u7684\u5206\u4eab\u6709\u4efb\u4f55\u7591\u95ee\uff0c\u6b22\u8fce\u5173\u6ce8\u516c\u4f17\u53f7\u5411\u5c0f\u679c\u63d0\u95ee\uff0c\u6211\u4eec\u6709\u4e13\u95e8\u7684\u751f\u4fe1\u5206\u6790\u670d\u52a1\u54e6\u3002\u540c\u65f6\uff0c\u5982\u679c\u5927\u5bb6\u6ca1\u6709\u505a\u51fa\u6765\u56fe\u4e5f\u4e0d\u8981\u7070\u5fc3\uff0c\u53ef\u4ee5\u8bd5\u4e00\u8bd5\u6211\u4eec\u7684\u4e91\u751f\u4fe1\u5c0f\u5de5\u5177\u54e6\uff0c\u53ea\u8981\u8f93\u5165\u5408\u9002\u7684\u6570\u636e\u4ee5\u53ca\u6307\u4ee4\u5c31\u53ef\u4ee5\u76f4\u63a5\u7ed8\u5236\u60f3\u8981\u7684\u56fe\u5462\uff0c\u94fe\u63a5\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002<\/span><\/span><\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><br \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0px;margin-bottom: 0px;\"><span style=\"color: rgb(0, 122, 170);\"><strong><span style=\"color: rgb(0, 122, 170);letter-spacing: 0.578px;text-indent: 32px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 15px;\">\u5c0f\u679c\u8fd8<span style=\"color: rgb(0, 122, 170);background-color: rgb(255, 255, 255);font-size: 15px;letter-spacing: 0.578px;text-decoration: none solid rgba(0, 0, 0, 0.9);\">\u63d0\u4f9b\u601d\u8def\u8bbe\u8ba1\u3001\u5b9a\u5236\u751f\u4fe1\u5206\u6790\u3001\u6587\u732e\u601d\u8def\u590d\u73b0\uff1b\u6709\u9700\u8981\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u76f4\u63a5\u626b\u7801\u54a8\u8be2\u5c0f\u679c\uff0c\u7aed\u8bda\u4e3a\u60a8\u7684\u79d1\u7814\u52a9\u529b\uff01<\/span><\/span><\/strong><\/span><\/p>\n<p style=\"margin-bottom: 0px;\"><br \/><\/p>\n<section style=\"font-size: 15px;padding-right: 2px;padding-left: 2px;margin-bottom: 0px;\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;flex: 0 0 auto;height: auto;border-color: rgb(114, 114, 114);border-width: 1px;border-style: solid;box-shadow: rgb(194, 209, 255) -5px 5px 0px;align-self: flex-start;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 42.6829%;vertical-align: middle;box-shadow: rgb(43, 65, 110) 0px 0px 0px;flex: 0 0 auto;height: auto;padding-left: 10px;align-self: center;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;line-height: 0;transform: translate3d(20px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 75%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015394\" data-ratio=\"1\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_B2jkicacLzPjw4xMSqVvoucZjonVDA-2.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxVYfSsM0wdf9d3RpNiaTcHohxPNEVs2VFhPMukpU8lchxrvcB2jkicacLzPjw4xMSqVvoucZjonVDA\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"430\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 56.9106%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"line-height: 2;letter-spacing: 1px;color: rgb(43, 65, 110);\" powered-by=\"xiumi.us\">\n<p style=\"margin-bottom: 2px;\"><strong>\u5b9a\u5236\u751f\u4fe1\u5206\u6790<\/strong><\/p>\n<p style=\"margin-bottom: 2px;\"><strong>\u670d\u52a1\u5668\u79df\u8d41<\/strong><\/p>\n<p><strong>\u626b\u7801\u54a8\u8be2\u5c0f\u679c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-wrap: wrap;\" powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497780&idx=2&sn=7eee90af6f387e4153bba13028e545a1&chksm=cf7f68f1f808e1e7dfb3a0597ef442e5c6e700ecf6ee5a035c766a4c7486c12eabd0ab218ea6&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100015395\" data-ratio=\"0.3253333333333333\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_7TvAyUibd1TPhJmZfialJtv6tu2SEg-5.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxVYfSsM0wdf9d3RpNiaTcHoj9xlsoyiaBvzaezDUFAicibMAF8ts7TvAyUibd1TPhJmZfialJtv6tu2SEg\/640?wx_fmt=png&from=appmsg'\" data-type=\"png\" data-w=\"750\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/span><\/a><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img js_insertlocalimg\" data-imgfileid=\"100015396\" data-ratio=\"0.3811111111111111\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/13_9N02aBaau07RufXeXRLmvAHsrFZxtw-2.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAz5wg6Ne6sYkannSE9NiatkcPh3icbKanJagacjU6LqJrGAL29N02aBaau07RufXeXRLmvAHsrFZxtw\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"900\" style=\"letter-spacing: 0.578px;text-align: center;text-wrap: wrap;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 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!important;border-right: 0.7em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"min-height: 40px;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"width: 100%;margin-right: auto;margin-bottom: -10px;margin-left: auto;\">\n<table width=\"100%\">\n<tbody>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:0\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:0.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;box-shadow: rgb(0, 0, 0) 0px 0px 0px;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>01<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497780&idx=2&sn=7eee90af6f387e4153bba13028e545a1&chksm=cf7f68f1f808e1e7dfb3a0597ef442e5c6e700ecf6ee5a035c766a4c7486c12eabd0ab218ea6&scene=21#wechat_redirect\" textvalue=\"1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff0c\u4e24\u4eba\u6210\u56e2\uff0c1\u4eba\u514d\u5355\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff0c\u4e24\u4eba\u6210\u56e2\uff0c1\u4eba\u514d\u5355\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:1\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:1.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>02<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498878&idx=1&sn=fc34ed3f1d15d060bbaf8166adcebd58&chksm=cf7f6cbbf808e5ad0b5417e50ed75cfbd1b3a83ac43b97f1dfa718c822878a9a004990075133&scene=21#wechat_redirect\" textvalue=\"\u5355\u4e2a\u6570\u636e\u5e93\u7528\u817b\u4e86\uff1f\u591a\u6570\u636e\u5e93\u201c\u7ec4\u5408\u62f3\u201d\u5e26\u4f60\u6253\u5f00\u514d\u75ab\u6d78\u6da6\u65b0\u601d\u8def\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u5355\u4e2a\u6570\u636e\u5e93\u7528\u817b\u4e86\uff1f\u591a\u6570\u636e\u5e93\u201c\u7ec4\u5408\u62f3\u201d\u5e26\u4f60\u6253\u5f00\u514d\u75ab\u6d78\u6da6\u65b0\u601d\u8def\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:2\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:2.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>03<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498673&idx=1&sn=1e09b74a836c1e64a62ae1f66eeb3f93&chksm=cf7f6b74f808e262de70be43fd73bf5fefdc0914e525eb2032f741a0152e619689cedc76946f&scene=21#wechat_redirect\" textvalue=\"\u5b5f\u5fb7\u5c14\u968f\u673a\u5316\u7684\u51c6\u5907\u5de5\u4f5c\uff0cGWAS\u6570\u636e\u7684\u7f51\u7ad9\u4e0b\u8f7d\u65b9\u6cd5\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"11\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u5b5f\u5fb7\u5c14\u968f\u673a\u5316\u7684\u51c6\u5907\u5de5\u4f5c\uff0cGWAS\u6570\u636e\u7684\u7f51\u7ad9\u4e0b\u8f7d\u65b9\u6cd5<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:3\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:3.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>04<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498517&idx=1&sn=fa3c0104cee316982fe8162b79fe234f&chksm=cf7f6bd0f808e2c6cf979704bf883cfb5480444604f90da61dc286e7541fa5bb49df0a2fb58a&scene=21#wechat_redirect\" textvalue=\"\u8ddf\u7740\u5c0f\u679c\u5b66\u590d\u73b0-\u624b\u628a\u624b\u5e26\u4f60\u62ff\u4e0bIF=46.9Nature \u7ea7\u522b\u7684\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u56fe\uff01\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u8ddf\u7740\u5c0f\u679c\u5b66\u590d\u73b0-\u624b\u628a\u624b\u5e26\u4f60\u62ff\u4e0bIF=46.9Nature \u7ea7\u522b\u7684\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u56fe\uff01\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: center;margin-bottom: 0px;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><o:page><\/o:page><\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5355\u7ec6\u80de\u8868\u578b\u9884\u6d4b\u9ed1\u79d1\u6280\uff013\u5206\u949f\u5e26\u4f60\u89e3\u9501ScPP\u5b8c\u5168\u4f53\uff01\uff01 \u5c0f\u679c  \u751f\u4fe1\u679c &nbs […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58291"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=58291"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58291\/revisions"}],"predecessor-version":[{"id":58305,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58291\/revisions\/58305"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=58291"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=58291"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=58291"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}