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decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014755\" data-ratio=\"0.24351851851851852\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_MT3apibaLcULmicticv7I56yHtsywQ.png?resize=553%2C134\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGQHA5RAyESZib8N6K107BSWIQgtDZMT3apibaLcULmicticv7I56yHtsywQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"134 .739990234375\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/span><\/p>\n\u5728\u8fd9\u91cc\u8f93\u5165\u6211\u4eec\u7684\u76f8\u5173\u7684\u4fe1\u606f\u641c\u7d22\uff0c\u8fd9\u91cc\u5c0f\u56fe\u8bd5\u9a8c\u80ba\u764c\u8fdb\u884c\u5c55\u793a\uff0clung cancer \u6211\u4eec\u5728\u641c\u7d22\u7684\u65f6\u5019\u540e\u9762\u8981\u52a0\u5165\u5355\u7ec6\u80de\u76f8\u5173\u7684\u5173\u952e\u5b57\uff0clung caner scRNAseq \u7136\u540e\u5728\u52a0\u4e0a human\u4eba\u7c7b\uff0c\u4e00\u822c\u5c31\u4f7f\u7528lung caner scRNAseq human \u5982\u679c\u662f\u5176\u4ed6\u75be\u75c5 \u6362\u4e00\u4e0b\u5c31\u53ef\u4ee5\uff0c\u6bd4\u5982\u5bab\u9888\u764c cervical cancer scRNAseq human<\/span><\/p>\n\u6765\u770b\u770b\u6548\u679c <\/span> <o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014759\" data-ratio=\"0.46111111111111114\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_ibDc1EOSibeicT3VFrnyAdTFbvgtIA.png?resize=553%2C255\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGyStUDY8TMvCIhibkxQ54WfsQZMX3ibDc1EOSibeicT3VFrnyAdTFbvgtIA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"255 .20001220703125\" width=\"553 .739990234375\" 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<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014756\" data-ratio=\"0.8270509977827051\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_ug8Jg3OJiccEGsA1YPutFZdXj64H5Q.png?resize=553%2C457\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGib4rJ5Ka8TwkiascxcbOqJKnoTDug8Jg3OJiccEGsA1YPutFZdXj64H5Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"902\" height=\"457 .94000244140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014757\" data-ratio=\"0.7972465581977471\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_kIUzNPwwL7uXnZoNmP9pV4RGZ0pb6g.png?resize=553%2C441\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGsrv6nOqibNW2PKNxic9JdUkMKCkIUzNPwwL7uXnZoNmP9pV4RGZ0pb6g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"799\" height=\"441 .469970703125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span> <o:page><\/o:page><\/p>\n\u8fd9\u91cc\u6709\u5f88\u591a\u5355\u7ec6\u80de\u6570\u636e\uff0c\u5176\u5b9e\u6211\u4eec\u9009\u62e9\u4e0b\u8f7d\u7684\u4e5f\u662f\u6709\u76ee\u7684\u7684\uff0c\u6211\u4eec\u5982\u679c\u9009\u62e9<\/span><img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014762\" data-ratio=\"0.22692307692307692\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_OjRIcJV0tZdIwxibcKes5ERjsVHHKw.png?resize=519%2C117\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGDXE511s2OnexNpREcxIdvSEEPOjRIcJV0tZdIwxibcKes5ERjsVHHKw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"520\" height=\"117 .989990234375\" width=\"519 .9400024414062\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e09\u4e2a\u91cc\u9762\u7684\u4e00\u79cd\uff0c\u70b9\u51fb\u8fdb\u53bb\u4e0b\u8f7d<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014764\" data-ratio=\"0.36561743341404357\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_NIjtQib3eiayYpsVlDeMWadS9FsZJA.png?resize=553%2C202\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGo7avX8gWKL9wIQkS9HsLf2BOayNIjtQib3eiayYpsVlDeMWadS9FsZJA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"826\" height=\"202 .47000122070312\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014760\" data-ratio=\"0.04980340760157274\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_yopfkHSGV16twFXABnRicboVmqtESQ.png?resize=553%2C27\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGEhVRO3jkP9ic8QpCJqCGHA2cTGyopfkHSGV16twFXABnRicboVmqtESQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"763\" height=\"27 .530029296875\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/span><\/p>\n\u4e0b\u8f7d\u8fd9\u4e2a\u5c31\u53ef\u4ee5\uff0c\u8fd9\u79cd\u662fCSV\u683c\u5f0f\u7684\uff0c\u4e0b\u9762\u5c0f\u679c\u4f1a\u6559\u5c0f\u4f19\u4f34\u5bfc\u5165\u6570\u636e\u683c\u5f0f\u7684\u65b9\u5f0f\uff0c\u5176\u5b9e\u6211\u4eec\u4e0b\u8f7d\u6570\u636e\u96c6\u53ef\u80fd\u8fd8\u6709\u5176\u4ed6\u6570\u636e\u683c\u5f0f\uff0c\u4e00\u822c\u6700\u591a\u7684\u5c31\u662fH5\u683c\u5f0f\uff0c\u8fd8\u6709mtx\u683c\u5f0f\uff0c\u8fd9\u91cc\u5c0f\u4f19\u4f34\u4e0d\u7528\u62c5\u5fc3\uff0c\u5c0f\u679c\u90fd\u4f1a\u6559\u5c0f\u4f19\u4f34\uff0c\u6211\u4eec\u6765\u770b\u4e00\u4e2aH5\u683c\u5f0f\u7684\uff0c<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014763\" data-ratio=\"0.23465211459754434\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_UL11RDbvn9E5Bje6kBkzycHQ3iamfA.png?resize=553%2C129\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGz1tHkn8xofPuNqAwXW7Tx9AeUUL11RDbvn9E5Bje6kBkzycHQ3iamfA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"733\" height=\"129 .94000244140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/span><\/p>\n\u4e0b\u8f7d\u8fd8\u89e3\u538b\u540e<\/span><\/p>\n<\/span><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014761\" data-ratio=\"0.18654434250764526\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_aD54fiaERkTXRBWXozbgSPfh3Tu5hg.png?resize=553%2C103\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGShKUJD3v7yflTD4L0Rj88P8ysaD54fiaERkTXRBWXozbgSPfh3Tu5hg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"654\" height=\"103 .27001953125\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/span><\/p>\n\u5c31\u4f1a\u8fd9\u6837\uff0c<\/span> <o:page><\/o:page><\/p>\n\u8fd8\u6709\u4e00\u79cd<\/span><\/p>\n<\/span><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014765\" data-ratio=\"0.08986928104575163\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_RnE1o8KZemsQiaIzqsOficmPickGCQ.png?resize=553%2C49\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGqHCcsFsxfa2mfrW3gFAD6KmfVNFRnE1o8KZemsQiaIzqsOficmPickGCQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"612\" height=\"49 .72999572753906\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u6211\u4eec\u4e0b\u9762\u5bf9\u8fd9\u4e09\u4e2a\u683c\u5f0f\u5bfc\u5165\u8fdb\u884c\u5b66\u4e60\uff0c<\/span> <\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"> <\/o:p><\/span><\/p>\n\u6211\u4eec\u5148\u4eceH5\u683c\u5f0f\u5b66\u4e60\uff0c\u8fd9\u4e2a\u6bd4\u8f83\u7b80\u5355\u4e00\u884c\u4ee3\u7801\u5c31\u5b66\u4f1a\u4e86\uff0c\u8fd9\u91cc\u5c0f\u679c\u968f\u4fbf\u7528\u4e00\u4e2a\u6570\u636e\u96c6\u5c55\u793a\uff0c<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>library(hdf5r)<\/span><\/code><code>library(Seurat)<\/span><\/code><code>library(dplyr)<\/span><\/span><\/code><\/pre>\n<\/section>\n\u6211\u4eec\u8fd8\u8981\u52a0\u8f7d\u4e00\u4e0b\u8fd9\u4e2a\u5305\uff0c\u7528\u6765\u5904\u7406\u6570\u636e\u7684<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code>data_sample<\/span> <- Read10X_h5(\"GSM4983457_NC_filtered_feature_bc_matrix.h5\"<\/span>) #\u5bfc\u5165H5\u683c\u5f0f\u6570\u636e<\/span><\/span><\/code><code>data_seurat <- CreateSeuratObject(data_sample,project = \"data_sample\"<\/span>) #\u540e\u9762\u5c31\u53ef\u4ee5\u8fdb\u884c\u5355\u7ec6\u80de\u5904\u7406\u7684\u6807\u51c6\u6d41\u7a0b\u4e86<\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014768\" data-ratio=\"0.11077844311377245\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_fSDeCAWiamf8z29Z8UvMXnR9brNACA.png?resize=553%2C61\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGyTyp9Dsv65YPrtibtA2LRIM3kvfSDeCAWiamf8z29Z8UvMXnR9brNACA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"668\" height=\"61 .27001953125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u6837\u5c31\u5bfc\u5165\u6210\u529f\u4e86\uff0c<\/span><\/p>\n\u4e0b\u9762\u6211\u4eec\u5bf9mtx\u683c\u5f0f\u8fdb\u884c\u5bfc\u5165\uff0c<\/span><\/p>\n\u800c\u6211\u4eec\u5f80\u5f80\u4e0b\u8f7dmtx\u683c\u5f0f\u7684\u4e00\u822c\u4f1a\u6709\u8fd9\u4e09\u4e2a\u6587\u4ef6<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014767\" data-ratio=\"0.17936507936507937\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/13_VAaWXUDHCeCBHpYZfCSGiaqCVWvpxA.png?resize=553%2C99\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGWrEzmNorq2ucukmCR67WDdcxmVAaWXUDHCeCBHpYZfCSGiaqCVWvpxA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"630\" height=\"99 .27001953125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code># \u8bfb\u53d610x\u6570\u636e\uff0cdata.dir\u53c2\u6570\u6307\u5b9a\u5b58\u653e\u6587\u4ef6\u7684\u8def\u5f84 <\/span><\/span><\/code><code>seurat_data<\/span> <- Read10X(data.dir = \"E:\\JYStudio\\Rtest\\Ztest1\\\u5355\u7ec6\u80de\\\u5355\u7ec6\u80de\u6570\u636e\u5904\u7406\\Tumor\"<\/span>)<\/span><\/code><code>##\u8fd9\u4e2a\u5c0f\u4f19\u4f34\u8981\u81ea\u884c\u8bbe\u7f6e\u5b58\u653e\u8fd9\u4e2a\u6587\u4ef6\u7684\u76ee\u5f55\uff0c<\/span><\/span><\/code><code># \u7136\u540e\u6211\u4eec\u521b\u5efaSeurat\u5bf9\u8c61<\/span><\/span><\/code><code>data_seurat <- CreateSeuratObject(counts = seurat_data,<\/span><\/code><code> project = \"GSM5155196_352\"<\/span>,<\/span><\/code><code> min.features = 200<\/span>,<\/span><\/code><code> min.cells = 3<\/span>)<\/span><\/code><code>\u8fd9\u4e2a\u5c31\u548c\u4e0a\u8ff0\u521b\u5efa\u5bf9\u8c61\uff0c\u540e\u7eed\u5c31\u53ef\u4ee5\u8fdb\u884c\u5355\u7ec6\u80de\u7684\u6807\u51c6\u6d41\u7a0b\u4e86<\/span><\/code><code>\u4f7f\u7528\u8fd9\u4e2a\u4ee3\u7801\u5c31\u53ef\u4ee5\u5bfc\u5165\u6570\u636e\uff0c<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014766\" data-ratio=\"0.11746987951807229\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/14_W9zyn1LMljVCx9Qy2prclk3iaxF8Ww.png?resize=553%2C65\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGG0bSZvTOG1zorJXALbkY2BtMaWW9zyn1LMljVCx9Qy2prclk3iaxF8Ww\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"664\" height=\"65 .0\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u5bfc\u5165\u540e\u5c31\u662f\u4e00\u6837\u7684\uff0c<\/span><\/p>\n\u6700\u540e\u4e00\u79cd\u5c31\u662fcsv\u683c\u5f0f\u7684\uff0c\u5c0f\u4f19\u4f34\u6709\u7684\u4e0b\u8f7d\u53ea\u6709csv\u683c\u5f0f\u7684\u600e\u4e48\u529e\uff0c\u5c0f\u679c\u8fd9\u91cc\u6559\u5927\u5bb6<\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"swift\"><code>##\u7b2c\u4e8c\u79cd<\/span><\/code><code>library(tidyr)<\/span><\/code><code>library(tidyverse)<\/span><\/code><code>library(org.Mm<\/span>.eg.db)<\/span><\/code><code>library(scater)<\/span><\/code><code>library(scran)<\/span><\/code><code>library(pheatmap)<\/span><\/code><code>library(limma) <\/span><\/code><code>library(edgeR)<\/span><\/code><code>library(dynamicTreeCut)<\/span><\/code><code>library(cluster)<\/span><\/code><code>library(monocle)<\/span><\/code><code>aa <- read.csv(\"GSE122340.csv\"<\/span>,row.names = 1<\/span>,header = T<\/span>)<\/span><\/code><code>all.counts <- as<\/span>.matrix(aa)<\/span><\/code><code>pbmc <- CreateSeuratObject<\/span>(counts = all.counts, project = \"TCGA\"<\/span>, names.delim = \"-\"<\/span>,<\/span><\/code><code> min<\/span>.cells = 3<\/span>, min<\/span>.features = 200<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n\u6211\u4eec\u628a\u5305\u52a0\u8f7d\u597d\u4e86\u4e4b\u540e\uff0c\u7136\u540e\u5bfc\u5165\u81ea\u5df1csv\u683c\u5f0f\u6587\u4ef6\uff0c\u7136\u540e\u5bfc\u5165\u6210\u529f\u540e\uff0c\u5c31\u53ef\u4ee5\u6b63\u5e38\u8fdb\u884c\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u4e86\uff01<\/span><\/p>\n\u597d\u4e86\u5173\u4e8e\u6570\u636e\u4e0b\u8f7d\u548c\u5bfc\u5165\u5c31\u5b8c\u6210\u4e86\uff0c\u5c0f\u4f19\u4f34\u5feb\u53bb\u52a8\u624b\u8bd5\u8bd5\uff0c\u4e0b\u9762\u6211\u4eec\u5c31\u5f00\u59cb\u6b63\u5f0f\u5206\u6790\u4e86\uff01<\/span><\/p>\n\u8fd9\u91cc\u5c0f\u679c\u628a\u5bfc\u5165\u6570\u636e\u5904\u7406\u4e00\u4e0b\uff1a<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code># \u67e5\u770bSeurat\u5bf9\u8c61\u7684\u57fa\u672c\u4fe1\u606f<\/span><\/span><\/code><code>data_seurat<\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014769\" data-ratio=\"0.16512345679012347\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/15_YHoZWB8cXBgAWjezOiavwvp7JhWOxQ.png?resize=553%2C91\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGowk2YzvfVIQ4VHK2ibF0DEO60ZYHoZWB8cXBgAWjezOiavwvp7JhWOxQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"648\" height=\"91 .4000244140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e2a\u5c31\u662f\u53ef\u4ee5\u770b\u5230\u6211\u4eec\u5bfc\u5165\u5355\u7ec6\u80de\u6570\u636e\u7684\u603b\u4f53<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"swift\"><code>data_seurat[[\"percent.mt\"<\/span>]]<-PercentageFeatureSet<\/span>(data_seurat,pattern = \"MT-\"<\/span>)<\/span><\/code><code> <\/span><\/code><code>VlnPlot<\/span>(data_seurat,features = c<\/span>(\"nCount_RNA\"<\/span>,\"nFeature_RNA\"<\/span>,\"percent.mt\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014771\" data-ratio=\"0.9801526717557252\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/16_pP8sv9aDHfsHhuPAFM7DULBWjsyalA.png?resize=553%2C542\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGGot9GDxoOOoKmUGrfXboYBNQpP8sv9aDHfsHhuPAFM7DULBWjsyalA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"655\" height=\"542 .739990234375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u6211\u4eec\u4ee5\u4e0d\u540c\u5f62\u5f0f\u5c55\u793a\u770b\u770b\u57fa\u56e0\u6570\u636e\u90a3\u4e9b\u7ec6\u80de\uff0c\u6bd4\u5982\u7ebf\u7c92\u4f53\u7b49<\/span><\/p>\n\u7136\u540e\u6211\u4eec\u8981\u5254\u9664\uff0c<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cpp\"><code>plot1<-FeatureScatter(data_seurat,feature1 = \"nCount_RNA\"<\/span>,feature2 = \"percent.mt\"<\/span>)<\/span><\/code><code>plot2<-FeatureScatter(data_seurat,feature1 = \"nCount_RNA\"<\/span>,feature2 = \"nFeature_RNA\"<\/span>)<\/span><\/code><code>CombinePlots(plots = list<\/span>(plot1,plot2)) <\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014770\" data-ratio=\"0.9909774436090225\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/17_vOH08ZhDC9ACu7tHNPR0ibcGGaZbjg.png?resize=439%2C435\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGg1uUjicxutaqBGXYgff6oFdWibRvOH08ZhDC9ACu7tHNPR0ibcGGaZbjg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"665\" height=\"435 .25\" width=\"439 .2099609375\" data-recalc-dims=\"1\" \/><\/span> <\/span><\/span><\/p>\n\u5254\u9664\u60c5\u51b5\uff0c\u8fd9\u4e2a\u65e0\u5173\u7d27\u8981\uff0c<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>pbmc<-subset(data_seurat,subset = nFeature_RNA>200 & nFeature_RNA<2500 & percent.mt<5)<\/span><\/span><\/code><\/pre>\n<\/section>\n\u8fd9\u4e2a\u6839\u636e\u5c0f\u4f19\u4f34\u81ea\u5df1\u7684\u60c5\u51b5\u53bb\u9009\u62e9\uff0c\u8303\u56f43000\u8fd8\u67095000 \u8fd8\u6709\u6700\u5c0f\u4e3a200\u8fd8\u662f\u591a\u5c11\uff0c\u5c0f\u4f19\u4f34\u53ef\u4ee5\u6839\u636e\u81ea\u8eab\u60c5\u51b5\uff0c<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code>ncol<\/span>(as<\/span>.data<\/span>.frame<\/span>(pbmc<\/span>[[\"RNA\"]<\/span>]@counts<\/span>))<\/span><\/code><code>pbmc<-NormalizeData(pbmc,normalization.method = \"LogNormalize\"<\/span>,scale.factor = 10000<\/span>)#\u5bf9\u6bcf\u4e2a\u7ec6\u80de\u6807\u51c6\u5316.<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014773\" data-ratio=\"0.18343815513626835\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/18_TSCIxmZuhOUdFm06jEzBJxRoXhic6w.png?resize=553%2C101\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGh9Wp33d14ZmLhxMDRahwLqkxxTSCIxmZuhOUdFm06jEzBJxRoXhic6w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"954\" height=\"101 .52996826171875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u6211\u4eec\u5bf9\u6bcf\u4e2a\u7ec6\u80de\u90fd\u8fdb\u884c\u6807\u51c6\u5316\uff0c<\/span> <o:page><\/o:page><\/p>\n\u4e0b\u9762\u6211\u4eec\u5bf9\u6570\u636e\u8fdb\u884c\u5904\u7406<\/span><\/p>\n\u8fd9\u6b21\u5c0f\u679c\u7ee7\u7eed\u6559\u5c0f\u4f19\u4f34\u5bf9\u6570\u636e\u5904\u7406\uff0c\u8fd8\u6709\u6211\u4eec\u7684\u91cd\u5934\u620f\u5bf9\u5355\u7ec6\u80de\u6ce8\u91ca\uff0c\u4e0b\u9762\u6211\u4eec\u5f00\u59cb\u5427<\/span><\/p>\n\u4e0a\u6b21\u6211\u4eec\u5904\u7406\u5230\uff0c<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014774\" data-ratio=\"0.1592920353982301\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/19_j2S19L1DBXQ6IBEPAR1GIylKTOdfkA.png?resize=553%2C88\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGQZplUtfQmGyiaicRMBLYTHzLvXj2S19L1DBXQ6IBEPAR1GIylKTOdfkA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1017\" height=\"88 .19999694824219\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u5bf9\u6bcf\u4e2a\u7ec6\u80de\u90fd\u8fdb\u884c\u4e86\u6807\u51c6\u5316\uff0c\u4e0b\u9762\u6211\u4eec\u5c31\u662f\u8be5\u5bfb\u627e\u9ad8\u53d8\u7684\u57fa\u56e0\uff0c\u8fd9\u6837\u624d\u80fd\u627e\u5230\u4e0e\u6211\u4eec\u6574\u4f53\u5355\u7ec6\u80de\u76f8\u5173\u7684\u57fa\u56e0\u4e4b\u95f4\u7684\u5173\u7cfb\uff0c\u6765\u770b\u770b\u5982\u4f55\u5bfb\u627e<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code>#\u5bfb\u627e\u9ad8\u53d8\u57fa\u56e0<\/span><\/span><\/code><code>pbmc<\/span><-FindVariableFeatures(pbmc,selection.method = \"vst\"<\/span>,nfeatures = 2000)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014772\" data-ratio=\"0.23094425483503983\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/20_Jz5QNyp4icBqArPJ3WXj3DhOg6viaQ.png?resize=553%2C127\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGbpx8W2EYg6lYa5MzDYsmWNMS4aJz5QNyp4icBqArPJ3WXj3DhOg6viaQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"879\" height=\"127 .8699951171875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u7136\u540e\u6211\u4eec\u8fdb\u884c<\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code>#PCA<\/span><\/span><\/code><code>top10<\/span><-head(VariableFeatures(pbmc))<\/span><\/code><\/pre>\n<\/section>\n\u8fd9\u884c\u4ee3\u7801\u6211\u4eec\u67e5\u770b\u4e00\u4e0b\u524d\u5341\u4e2a\u7684\u9ad8\u53d8\u57fa\u56e0\uff0c\u53ef\u4ee5\u53d1\u73b0\u6211\u7684\u8fd9\u4e2a\u53ea\u67096\u4e2a\u9ad8\u53d8\u57fa\u56e0\uff0c\u53ef\u80fd\u6211\u9009\u53d6\u7684\u6570\u503c\u6bd4\u8f83\u5c11\uff0c\u4e0d\u8fc7\u4e0d\u5f71\u54cd<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014775\" data-ratio=\"0.08728943338437979\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/21_N5BuVUfqnOyEDoJvF95r1BUeoOXg8Q.png?resize=553%2C48\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGG1ibzZjgrjKZlfFErjqkn27XRibN5BuVUfqnOyEDoJvF95r1BUeoOXg8Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"653\" height=\"48 .27001953125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u6211\u4eec\u7ee7\u7eed\u5f80\u4e0b\u8d70<\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code>plot1<-VariableFeaturePlot(pbmc)<\/span><\/span><\/code><code>plot2<-LabelPoints(plot<\/span> = plot1,points=top10,repel=T)<\/span><\/span><\/code><code>plot1<\/span> <\/span><\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014777\" data-ratio=\"1.0062402496099845\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/22_n8Xqq5lm8ddj9OFibibo6J1iaC1Dzg.png?resize=553%2C557\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGVs1373mvD8iauMbBYsokrw5rr3G9n8Xqq5lm8ddj9OFibibo6J1iaC1Dzg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"641\" height=\"557 .2100219726562\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n <o:p> <\/o:p><\/span><\/p>\nplot2<\/span> <o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014776\" data-ratio=\"1.0404984423676011\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/23_8vZJSeyibHibNicZIrbuVdv5aaZAhw.png?resize=553%2C576\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGbVM1icqoSRGnN72AGTBFicr0xaDgg8vZJSeyibHibNicZIrbuVdv5aaZAhw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"642\" height=\"576 .1400146484375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u628a\u6211\u4eec\u627e\u5230\u7684\u9ad8\u53d8\u57fa\u56e0\u653e\u5728\u706b\u5c71\u56fe\u4e0a\u9762\uff0c\u6211\u4eec\u5bfb\u627e\u9ad8\u53d8\u57fa\u56e0\u4e5f\u662f\u4e3a\u4e86\u5728\u540e\u7eed\u5bfb\u627e\u7ec6\u80de\u4e9a\u7fa4\u7684\u65f6\u5019\u5206\u79bb\u6709\u7528\uff0c\u6211\u4eec\u540e\u7eed\u518d\u8bf4\uff0c\u4e0b\u9762\u6211\u4eec\u5bf9\u57fa\u56e0\u8fdb\u884c\u6807\u51c6\u5316\uff0c\u548c\u4e0a\u9762\u4e0d\u540c\u8fd9\u6b21\u662f\u5bf9\u57fa\u56e0<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code>all<\/span>.genes<-rownames(pbmc)<\/span><\/code><code>pbmc<\/span><-ScaleData(pbmc,features = all<\/span>.genes)#\u6bcf\u79cd\u57fa\u56e0\u5728\u7ec6\u80de\u4e2d\u7684\u6807\u51c6\u5316<\/span><\/code><code>pbmc<\/span> <- ScaleData(pbmc, vars.to.regress = \"percent.mt\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014778\" data-ratio=\"0.21203703703703702\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/24_hdzqWSHDT1mPthCSQEY8HVs2rFqFwA.png?resize=553%2C117\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGN0SLiaC5cw2VdYConnJ9dn55ehdzqWSHDT1mPthCSQEY8HVs2rFqFwA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"117 .5999755859375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span> <o:page><\/o:page><\/p>\n\u8fd9\u91cc\u5206\u6790\u597d\u4e86\uff0c\u6211\u4eec\u5c31\u53ef\u4ee5\u540e\u7eed\u5206\u6790\uff0c\u5bf9\u7ec6\u80de\u8fdb\u884c\u805a\u7c7b\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>pbmc<-RunPCA(pbmc,features<\/span> = VariableFeatures(object<\/span> = pbmc))<\/span><\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014779\" data-ratio=\"0.3206627680311891\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/25_tSdricRsp00R0ViaufOlibno4kEXyg.png?resize=553%2C177\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGvnecDy0I74jDzFutwUib4C6yTClptSdricRsp00R0ViaufOlibno4kEXyg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1026\" height=\"177 .53997802734375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u5728\u505a\u4e00\u4e2aPCA\u5206\u6790\uff0c\u770b\u770b\u4e0d\u540c\u7c7b\u4e4b\u95f4\u7684\u5173\u8054<\/span> <\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"> <\/o:p><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>print<\/span>(pbmc[[\"pca\"<\/span>]],dims=1:5,nfeatures=5)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014782\" data-ratio=\"0.6183986371379898\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/26_ibm2BMicr8BQbDia1YqtbicQtEUSPw.png?resize=553%2C342\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGfAYAwMegicAWwtaEU0J0asuRYkCQWibm2BMicr8BQbDia1YqtbicQtEUSPw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"587\" height=\"342 .469970703125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"javascript\"><code>VizDimLoadings(pbmc,dims = 1<\/span>:2<\/span>,reduction = \"pca\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014784\" data-ratio=\"1.0061919504643964\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/27_jRGKOiakaygCWORpVAodCEDxLFAv0A.png?resize=553%2C557\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGibRDaic61LUU7ibBt0rN0Euib733KjRGKOiakaygCWORpVAodCEDxLFAv0A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"646\" height=\"557 .1400146484375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"javascript\"><code>DimPlot(pbmc,reduction = \"pca\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014780\" data-ratio=\"1.0257234726688103\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/28_WNkCfJMOC39Y4MRiaZaicjsbxq5VdA.png?resize=553%2C568\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGG9ia0pyemViaXTc0jf5jsoc8p4RA0WNkCfJMOC39Y4MRiaZaicjsbxq5VdA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"622\" height=\"568 .010009765625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>DimHeatmap(pbmc,dims=1,cells=500,balanced = T)<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014781\" data-ratio=\"1.0207336523125996\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/29_8WQj8Rfvp6cRN8JvibPJo2RboWGibg.png?resize=553%2C565\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGFES4CyPibsLsdFzWlib5TibZ7AM1P8WQj8Rfvp6cRN8JvibPJo2RboWGibg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"627\" height=\"565 .2100219726562\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/p>\n\u8fd9\u4e2a\u5c31\u53ef\u4ee5\u770b\u5230\u6bcf\u4e2a\u57fa\u56e0\u5728\u4e0d\u540c\u7684\u4e9a\u7fa4\u4e4b\u95f4\u7684\u8868\u8fbe\u5982\u4f55\uff0c\u8fd8\u53ef\u4ee5\u5206\u5f00\u6765\u770b<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>DimHeatmap(pbmc,dims = 1:15,cells=500,balanced = T)<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014783\" data-ratio=\"1.0234375\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/30_f6qeE4nwsj8wqNibGt0qKZIhVlUdGA.png?resize=553%2C566\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGv1OfDElic5pd3zOjo7nt7OnWf6f6qeE4nwsj8wqNibGt0qKZIhVlUdGA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"640\" height=\"566 .739990234375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e2a\u5c31\u53ef\u4ee5\u770b\u51fa\u6765\u4e00\u4e9b\u57fa\u56e0\u5728\u4e0d\u540c\u4e9a\u7fa4\u7684\u8868\u8fbe\u91cf\uff0c\u4f46\u662f\u6211\u4eec\u4e0d\u77e5\u9053\u5206\u591a\u5c11\u5408\u9002\u5462\uff0c\u8fd9\u91cc\u5c31\u9700\u8981\u8fdb\u884c\u540e\u7eed\u7684\u5206\u6790\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>pbmc<-JackStraw(pbmc,num.replicate<\/span> = 100)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014785\" data-ratio=\"0.1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/31_SmtbF6HXiazyAicVmouAjpEic0Uibg.png?resize=553%2C55\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGN1cUYHe21Frq6ddw4XfuibqJKdeOYSmtbF6HXiazyAicVmouAjpEic0Uibg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"760\" height=\"55 .33001708984375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e00\u6b65\u5c31\u662f\u5206\u79bb\u5206\u6790\uff0c\u5c0f\u4f19\u4f34\u8010\u5fc3\u7b49\u5f85\u4e00\u4e0b<\/span><\/p>\n\u5c31\u662f\u5728\u5bfb\u627e\u6700\u4f73\u5206\u7fa4<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code>pbmc<-ScoreJackStraw(pbmc,dims=1:20)<\/span><\/span><\/code><code>JackStrawPlot(pbmc,dim=1:20)<\/span><\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014787\" data-ratio=\"0.12771739130434784\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/32_u0310oJ6rJib6HpjibTCNrOHicjSfg.png?resize=553%2C70\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGcVJFwdQiaBdJJNemSI5ibGOBhnIEtu0310oJ6rJib6HpjibTCNrOHicjSfg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"736\" height=\"70 .66999816894531\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014788\" data-ratio=\"0.9984639016897081\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/33_8ukMaVLp0Ba3wNSxDF0ZaX9hTpfTlw.png?resize=553%2C552\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGNGqCtE7zXdFc5BbmPe8IHZ0ic8ukMaVLp0Ba3wNSxDF0ZaX9hTpfTlw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"651\" height=\"552 .8800048828125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e2a\u5c31\u662f\u4e0d\u540c\u4e9a\u7fa4\u7684P\u503c\u60c5\u51b5\uff0c<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>ElbowPlot(pbmc)<\/span><\/code><\/pre>\n<\/section>\n<span style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;font-family: \u7b49\u7ebf;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;\"> <o:p> <\/o:p><\/span> <\/span><\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014786\" data-ratio=\"0.9818181818181818\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/34_8JdYTM1J9TnCUKTf9R5CIdnmicyXJA.png?resize=442%2C434\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGticSwQ2jibtQeG0u1bwxvI4fJW98JdYTM1J9TnCUKTf9R5CIdnmicyXJA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"660\" height=\"434 .65997314453125\" width=\"442 .7099609375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e2a\u788e\u77f3\u56fe\u6211\u4eec\u5c31\u53ef\u4ee5\u770b\u770b\u5728\u54ea\u91cc\u5e73\u7f13\u6211\u4eec\u5c31\u9009\u62e9\u54ea\u91cc\uff0c\u5c0f\u56fe\u8fd9\u5f20\u56fe\u5c31\u9009\u62e917\u6216\u800515\u90fd\u53ef\u4ee5<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code>pbmc<-FindNeighbors(pbmc,dims=1:17)<\/span><\/span><\/code><code>pbmc<-FindClusters(pbmc,resolution = 1.2)#0.4-1.2\u4e4b\u95f4\uff0c\u6570\u503c\u8d8a\u5927\u5206\u7fa4\u8d8a\u591a<\/span><\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014789\" data-ratio=\"0.41656942823803966\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/35_A4Mdj51eMP5Xxtj9u8lhTkicnUD6cA.png?resize=553%2C230\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGIrAqmMPiagYibrOuIgaUicfr4wfOA4Mdj51eMP5Xxtj9u8lhTkicnUD6cA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"857\" height=\"230 .66998291015625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>head(Idents(pbmc), 5) <span style=\"color: rgba(0, 0, 0, 0.9);font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: var(--articleFontsize);letter-spacing: 0.034em;text-align: justify;\"> <\/span><span style=\"color: rgba(0, 0, 0, 0.9);font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: var(--articleFontsize);letter-spacing: 0.034em;text-align: justify;\"><\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014790\" data-ratio=\"0.09537037037037037\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/36_DJOdN37rchHVdm499qazkoKJPlDpzg.png?resize=553%2C52\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGVEXM1c3SnzPh8RzEhDV5rBOjDJOdN37rchHVdm499qazkoKJPlDpzg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"52 .72999572753906\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u53ef\u4ee5\u770b\u51fa\u6765\u6211\u4eec\u5206\u4e869\u4e2a\u4e9a\u7fa4<\/span><\/p>\n\u7136\u540e\u6211\u4eec\u8fdb\u884c\u964d\u7ef4<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>pbmc<-RunUMAP(pbmc,dims=1:17)#\u4e00\u79cd\u964d\u7ef4\u65b9\u6cd5<\/span><\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014791\" data-ratio=\"0.38799414348462663\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/37_cK6fhXf4cz0ia9Pn7iadTiajkjyb2Q.png?resize=553%2C214\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGrDW9ibAcO2pLicNicyURSFvCZia0yib8cK6fhXf4cz0ia9Pn7iadTiajkjyb2Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"683\" height=\"214 .79998779296875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>DimPlot(pbmc,reduction = \"umap\"<\/span>)#umap\u7528\u4e8e\u5927\u578b\u6570\u636e<\/span><\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n\u7136\u540e\u540e\u9762\u6211\u4eec\u5c31\u8981\u8fdb\u884c\u964d\u7ef4\u7ed8\u5236\u56fe\uff0c<\/span> <o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014792\" data-ratio=\"1.0015384615384615\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/38_2SbsbVZcJrNlzEknzAA98tGso7OEjA.png?resize=553%2C554\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGjkJj4LicBmCawXCSH2mCzdiaH22SbsbVZcJrNlzEknzAA98tGso7OEjA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"650\" height=\"554 .6099853515625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u7528\u7684\u662fumap\u65b9\u5f0f\u8fdb\u884c\u53ef\u89c6\u5316\u964d\u7ef4\u7ed3\u679c\uff0c\u5bf9\u4e8e\u5927\u6570\u636e\u6765\u8bf4\u662f\u6bd4\u8f83\u9002\u7528\u7684\uff0c\u6211\u4eec\u8fd8\u53ef\u4ee5\u8fdb\u884c\u5176\u4ed6\u65b9\u5f0f\uff0c\u6bd4\u5982tsne\uff0c\u9002\u5408\u5c0f\u6570\u636e<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>pbmc<-RunTSNE(pbmc,dims=1:17)#\u53e6\u4e00\u79cd\u964d\u7ef4\u65b9\u6cd5<\/span><\/span><\/code><code>DimPlot(pbmc,reduction = \"tsne\"<\/span>)#tsne\u7528\u4e8e\u5c0f\u578b\u6570\u636e\uff0c\u5206\u79bb\u6548\u679c\u66f4\u597d<\/span><\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014793\" data-ratio=\"1.0031446540880504\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/39_BicCjysrVZ9LS2KtLqPq6ORI8vr8YA.png?resize=553%2C555\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGIjsmeiapOh5zprbeUwIdk9jAEeBicCjysrVZ9LS2KtLqPq6ORI8vr8YA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"636\" height=\"555 .47998046875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e2a\u5c31\u53ef\u4ee5\u770b\u51fa\u6765\uff0c\u5c0f\u679c\u7684\u6570\u636e\u662f\u5c0f\u578b\u6570\u636e\uff0c\u6240\u4ee5\u5462\uff0c\u5728\u4f7f\u7528tsne\u65b9\u5f0f\u7684\u66f4\u660e\u663e\u7684\u5206\u79bb\u5355\u7ec6\u80de\u4e9a\u7fa4<\/span><\/p>\n\u6700\u540e\u5462\u5c31\u662f\u9700\u8981\u627e\u5230\u6211\u4eec\u7684\u5404\u7c7b\u7684\u5173\u952e\u57fa\u56e0<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>#\u627e\u5404\u7c7b\u5173\u952e\u57fa\u56e0<\/span><\/code><code>pbmc.markers<-FindAllMarkers(pbmc,only.pos<\/span> = T,min.pct<\/span> = 0.25,logfc.threshold<\/span> = 0.25)#\u5bfb\u627e\u6240\u6709\u7c7b\u7684makers\u57fa\u56e0<\/span> <\/span><\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014794\" data-ratio=\"0.3141509433962264\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/40_dtX5AH8WWRxfT5MWyvkIsV319KZFdg.png?resize=553%2C173\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGw2QIRWWFG2dIsga7kmM2Bsn1dtX5AH8WWRxfT5MWyvkIsV319KZFdg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1060\" height=\"173 .94000244140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code>pbmc.markers%>%group_by(cluster) %>% top_n(n=2<\/span>,wt=avg_log2FC)<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014798\" data-ratio=\"0.4679334916864608\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/41_FvkzfwrndScaeB9yDZLVzaTWojwiaQ.png?resize=553%2C259\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGQMFme4gxjsLuzZPrB83BWib9iaKFvkzfwrndScaeB9yDZLVzaTWojwiaQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"842\" height=\"259 .1399841308594\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u5bf9\u7ed3\u679c\u5c55\u793a\uff0c\u4e00\u4e9b\u5173\u952e\u57fa\u56e0\uff0c\u6211\u4eec\u53ef\u4ee5\u9009\u62e9\u524d\u5341\u540d\u7684\u5c55\u793a\u5176\u5728\u5355\u7ec6\u80de\u4e9a\u7fa4\u4e2d\u7684\u5f62\u5f0f<\/span> <\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"> <\/o:p><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>VlnPlot(pbmc,features = c(\"GPX2\"<\/span>,\"AKR1C2\"<\/span>),slot = \"counts\"<\/span>,log<\/span>=T)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n\u5c0f\u56fe\u8fd9\u91cc\u5bf9\u524d\u4e24\u540d\u7684\u57fa\u56e0\u8fdb\u884c\u5c55\u793a<\/span> <o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014795\" data-ratio=\"0.9984375\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/42_PyjwmPSboYJ0UDnFErlqPCeibxFbag.png?resize=553%2C552\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGFmYM0QFjzRSN40o0XpkeTDzW8PyjwmPSboYJ0UDnFErlqPCeibxFbag\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"640\" height=\"552 .8800048828125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"swift\"><code>FeaturePlot<\/span>(pbmc,features = c<\/span>(\"GPX2\"<\/span>),reduction = \"tsne\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014796\" data-ratio=\"1.0063492063492063\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/43_I6S6nUhnb65icZx6pYm4boSt4LK59w.png?resize=421%2C424\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGHD6VgHST6ibB7wicmCmHwZsJRMBI6S6nUhnb65icZx6pYm4boSt4LK59w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"630\" height=\"424 .6400146484375\" width=\"421 .95001220703125\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u5c31\u53ef\u4ee5\u5f88\u660e\u663e\u7684\u770b\u51faGPX2\u8fd9\u4e2a\u57fa\u56e0\u5728\u90a3\u4e9b\u7ec6\u80de\u4e2d\u5360\u6bd4\u6700\u591a\uff0c<\/span><\/p>\nreduction = “tsne”\u8fd9\u4e2a\u53c2\u6570\u53ef\u4ee5\u6539\u53d8\u7ed8\u56fe\u7684\u65b9\u5f0f\uff0c<\/span><\/p>\n\u7136\u540e\u6211\u4eec\u5bf9\u524d\u5341\u540d\u7684\u8fdb\u884c\u4fdd\u5b58<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code>top10<\/span><-pbmc.markers%>%group_by(cluster) %>% top_n(n=10<\/span>,wt=avg_log2FC)<\/span><\/code><code>DoHeatmap(pbmc,features = top10<\/span>$gene)+NoLegend()<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014799\" data-ratio=\"1.0156985871271587\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/44_8m6UrZrIyuuL1DBBONENFg0nuMNe3A.png?resize=553%2C562\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGp7snxbeAjfVTDTvToYiaV6XVd8m6UrZrIyuuL1DBBONENFg0nuMNe3A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"637\" height=\"562 .4099731445312\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u5bf9\u5173\u952e\u57fa\u56e0\u8868\u8fbe\u91cf\u8fdb\u884c\u70ed\u56fe\u7684\u7ed8\u5236\uff0c\u770b\u770b\u8fd9\u4e9b\u57fa\u56e0\u5728\u90a3\u4e2a\u4e9a\u7fa4\u4e2d\u8868\u8fbe\u5982\u4f55\uff0c<\/span><\/p>\nsaveRDS(pbmc,”pbmc.rds”)<\/span><\/p>\n\u6700\u540e\u6211\u4eec\u4fdd\u5b58\u4e00\u4e0b\u8fd9\u4e9b\u4fe1\u606f\uff0c<\/span><\/p>\n\u7136\u540e\u6211\u4eec\u5bf9\u8fd9\u4e9b\u7ec6\u80de\u8fdb\u884c\u6ce8\u91ca\uff0c\u8fd9\u91cc\u6211\u4eec\u4f7f\u7528SingleR\u5305\u8fdb\u884c\u81ea\u52a8\u6ce8\u91ca\uff0c\u6bd4\u8f83\u65b9\u4fbf\uff0c\u5c0f\u679c\u4ee5\u53ca\u628a\u8fd9\u4e2a\u6570\u636e\u7684\u4fe1\u606f\u4e0b\u8f7d\u4e0b\u6765\u4e86\uff0c\u5c0f\u4f19\u4f34\u53ef\u4ee5\u81ea\u884c\u53bb\u540e\u53f0\u9886\u53d6<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code>library(SingleR)<\/span><\/code><code>#<\/span>BiocManager::install(\"celldex\"<\/span>)<\/span><\/code><code>library(celldex) <\/span><\/code><code>library(Seurat)<\/span><\/code><code>library(pheatmap)<\/span><\/code><\/pre>\n<\/section>\n\u52a0\u8f7d\u4e00\u4e0b\u5305\uff0c\u6211\u4eec\u8fdb\u884c\u5355\u7ec6\u80de\u6ce8\u91ca<\/span><\/p>\n##\u4e0b\u8f7d\u6ce8\u91ca\u6570\u636e\u5e93\uff0c\u8fd9\u91cc\u627e\u5c0f\u679c\u9886\u53d6\u4e0e\u559c\u7231\u4e0b\u8f7d\u597d\u7684\u6570\u636e\u5e93\u4fe1\u606f<\/span><\/p>\n\u5c0f\u4f19\u4f34\u4e5f\u53ef\u4ee5\u81ea\u884c\u53bb\u4e0b\u8f7d\uff0c\u4e0d\u8fc7\u4e00\u822c\u4f1a\u56e0\u4e3a\u7f51\u7edc\u95ee\u9898\u5931\u8d25\uff0c<\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code>hpca<\/span>.se<\/span> <-<\/span> HumanPrimaryCellAtlasData<\/span>()<\/span><\/code><code>hpca<\/span>.se<\/span><\/span><\/code><code> <\/span><\/code><code> <\/span><\/code><code>#\u76f4\u63a5load<\/span>\u4e0b\u8f7d\u597d\u7684\u6570\u636e\u5e93<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code>load<\/span>(\"HumanPrimaryCellAtlas_hpca<\/span>.se_human<\/span>.RData<\/span>\")<\/span><\/code><code> <\/span><\/code><code>load<\/span>(\"BlueprintEncode_bpe<\/span>.se_human<\/span>.RData<\/span>\")<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014797\" data-ratio=\"0.10063897763578275\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/45_cgvLsRrUGqp68nebfHrADeV3rzO4VQ.png?resize=553%2C55\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGpgH6l4NdKJLVa7iaPiaIpvXETPcgvLsRrUGqp68nebfHrADeV3rzO4VQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"626\" height=\"55 .66998291015625\" width=\"553 .739990234375\" data-recalc-dims=\"1\"> <\/span><o:p style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;\"> <\/o:p><\/span><\/p>\npbmc#\u4e0a\u9762\u5c0f\u679c\u5904\u7406\u597d\u7684\u5355\u7ec6\u80de\u4fe1\u606f<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014800\" data-ratio=\"0.17565485362095531\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/46_9ozS4MXjwDLfLiaoDuHNYAguG27lGA.png?resize=553%2C97\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGS1icULtuBicuKMP5SJlGZQdXV2s9ozS4MXjwDLfLiaoDuHNYAguG27lGA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"649\" height=\"97 .20001220703125\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code>meta=pbmc@meta.data #pbmc\u7684meta\u6587\u4ef6\uff0c\u5305\u542b\u4e86seurat\u7684\u805a\u7c7b\u7ed3\u679c <\/span><\/span><\/code><code>head(meta)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014802\" data-ratio=\"0.3614931237721022\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/47_q1YJmXNRGnwHiawvibNZxXzhvKic1Q.png?resize=553%2C200\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGlTtMOOH9QMiaJzNEZWb5xqoPLzQbq1YJmXNRGnwHiawvibNZxXzhvKic1Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1018\" height=\"200 .1400146484375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u67e5\u770b\u4e00\u4e0b\u4fe1\u606f<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code>plot1 <- DimPlot(pbmc, reduction = \"umap\"<\/span>, label = TRUE<\/span>)<\/span><\/code><code> <\/span><\/code><code>plot2<-DimPlot(pbmc, reduction = \"tsne\"<\/span>,<\/span><\/code><code> label = TRUE<\/span>)<\/span><\/code><code>plot1 + plot2<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014803\" data-ratio=\"0.7731481481481481\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/48_hk4O6GcVWA9nQ5nZaicKE4XbNibXxw.png?resize=553%2C427\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGG0M27NtnyogjJ93GRvrmnlCibHgPhk4O6GcVWA9nQ5nZaicKE4XbNibXxw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"427 .94000244140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u7136\u540e\u6211\u4eec\u5c31\u5f00\u59cb\u6ce8\u91ca\u5427\uff01<\/span><\/p>\n <o:p> <\/o:p><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>#\u8fdb\u884csingleR\u6ce8\u91ca<\/span><\/span><\/code><code>pbmc_for_SingleR <- GetAssayData(pbmc, slot=\"data\"<\/span>) ##\u83b7\u53d6\u6807\u51c6\u5316\u77e9\u9635<\/span><\/span><\/code><code>pbmc.hesc <- SingleR(test<\/span> = pbmc_for_SingleR, ref = hpca.se, labels = hpca.se$label<\/span>.main) #<\/span><\/span><\/code><code>pbmc.hesc<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014804\" data-ratio=\"0.37648327939590076\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/49_MhHTwlQyT8q1BoTaMr7ic4vfBWhxlw.png?resize=553%2C208\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGz1pz562CM6h27FsEkYkVH4p9FMhHTwlQyT8q1BoTaMr7ic4vfBWhxlw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"927\" height=\"208 .47000122070312\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>#seurat \u548c singleR\u7684table\u8868<\/span><\/span><\/code><code>table(pbmc.hesc$labels<\/span>,meta$seurat_clusters<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014801\" data-ratio=\"0.42219020172910665\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/50_LmSzuSpDNLzbEX57Y5mpVrE0ChJE1A.png?resize=553%2C233\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGZNLLuvMCicwaKSyRw9X0VK3odLmSzuSpDNLzbEX57Y5mpVrE0ChJE1A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"694\" height=\"233 .79998779296875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u6ce8\u91ca\u7ed3\u679c<\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code>#umap\u56fe<\/span><\/span><\/code><code> <\/span><\/code><code>pbmc@meta.data$labels <-pbmc.hesc$labels<\/span><\/code><code> <\/span><\/code><code> <\/span><\/code><code>print<\/span>(DimPlot(pbmc, group.by = c(\"seurat_clusters\"<\/span>, \"labels\"<\/span>),reduction = \"umap\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014809\" data-ratio=\"0.7675925925925926\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/51_ia1Micpia4WMSDUWz4ic1Ixu3VWB1A.png?resize=553%2C425\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGlcpOyJnP5TpZcVxeccic9a9vMqKWPia1Micpia4WMSDUWz4ic1Ixu3VWB1A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"425 .1400146484375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u6211\u4eec\u6ce8\u91ca\u5b8c\u6210\u4e86\uff01<\/span><\/p>\n\u4f46\u662f\u6211\u4eec\u8fd8\u9700\u8981\u591a\u4e2a\u6570\u636e\u5e93\u9a8c\u8bc1\u4ee5\u53ca\u5bf9\u7ed3\u679c\u8bca\u65ad\uff0c\u6765\u770b\u770b\uff1a<\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code>##\u591a\u4e2a\u6570\u636e\u5e93\u6ce8\u91ca<\/span><\/span><\/code><code>pbmc3 <- pbmc<\/span><\/code><code> <\/span><\/code><code>pbmc3.hesc <- SingleR(test = pbmc_for_SingleR, ref = list<\/span>(BP=bpe.se, HPCA=hpca.se),<\/span><\/code><code> labels = list<\/span>(bpe.se$label.main, hpca.se$label.main))<\/span><\/code><code>table(pbmc3.hesc$labels,meta$seurat_clusters)<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014807\" data-ratio=\"0.4830028328611898\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/52_apfF83IxmFfSanrPAPTtrxAxXgFcUg.png?resize=553%2C267\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGfiavUkEl3f7sDyVlvhKiabUTbMiapfF83IxmFfSanrPAPTtrxAxXgFcUg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"706\" height=\"267 .4700012207031\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code>pbmc3@meta.data$labels <-pbmc3.hesc$labels<\/span><\/code><code> <\/span><\/code><\/pre>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code>print(DimPlot(pbmc3, group<\/span>.by<\/span> = c(\"seurat_clusters\"<\/span>, \"labels\"<\/span>),reduction = \"tsne\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014806\" data-ratio=\"1.0093457943925233\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/53_FEVJ4ibvQVfEicqFQnPrP2VpYEAf4w.png?resize=553%2C558\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGQ6jic16gt1njLvWjTx9icLS5Y8ib4FEVJ4ibvQVfEicqFQnPrP2VpYEAf4w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"642\" height=\"558 .93994140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>#\u7ed3\u679c\u8bca\u65ad<\/span><\/span><\/code><code>print<\/span>(plotScoreHeatmap(pbmc.hesc))<\/span><\/code><code> <\/span><\/code><code>plotDeltaDistribution(pbmc.hesc, ncol = 3)<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014808\" data-ratio=\"0.7861111111111111\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/54_JdnAnlHXu2F0CslH0vNXBrfMHicS5g.png?resize=553%2C435\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGSQD1SF51d3arYeMKVVGhGQIMicJdnAnlHXu2F0CslH0vNXBrfMHicS5g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"435 .34002685546875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code>#cluster\u7ed3\u679c\u5bf9\u6bd4<\/span><\/span><\/code><code> <\/span><\/code><code>tab<\/span> <- table(label = pbmc.hesc$labels<\/span>,<\/span><\/code><code> <\/span><\/code><code> cluster = meta$seurat_clusters<\/span>)<\/span><\/code><code> <\/span><\/code><code>pheatmap(log10(tab + 10<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014805\" data-ratio=\"0.9863429438543247\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/55_cmcubZuSw3WM9ic1WH6RFjicWTPiaA.png?resize=553%2C546\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGdLRFLruwOCt9CfzaqwePFIbEvQkicmcubZuSw3WM9ic1WH6RFjicWTPiaA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"659\" height=\"546 .2100219726562\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u597d\u4e86\uff0c\u5230\u6b64\u6211\u4eec\u7684\u5355\u7ec6\u80de\u5165\u95e8\u5b66\u4e60\u7684\u6559\u7a0b\u5c31\u7ed3\u675f\u4e86\uff0c\u5c0f\u4f19\u4f34\u8981\u597d\u597d\u6d88\u5316\u4e00\u4e0b\uff0c\u5bf9\u6b64\u6570\u636e\u8fdb\u884c\u4eb2\u624b\u64cd\u4f5c\uff01<\/span><\/p>\n <\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100014811\" data-ratio=\"0.5555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/56_VmRkv7tSNKliafDnsuFR7BoeDgBrww.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxPqeNQsFjm2HibIuSld9Nh9GicQSWhJwHjia5tEevQvDIebLErVmRkv7tSNKliafDnsuFR7BoeDgBrww\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"900\" style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;\" data-recalc-dims=\"1\" \/> <\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"margin-top: 0pt;margin-bottom: 0pt;margin-left: 0pt;letter-spacing: 0.578px;text-indent: 0pt;text-wrap: wrap;text-align: justify;line-height: normal;font-size: 12pt;font-family: helvetica;color: rgb(0, 0, 0);break-after: auto;\"><\/span><\/span><span style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;color: rgba(0, 0, 0, 0.9);font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;text-align: justify;\"><\/span><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014812\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/57_FbEg9fibwQicL8l2ExKOZZTmAHXabQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAx3HetBkB7aOGibFWKqibqIATyC4zFibzCgdrxFwRep6cDMLIbA0FbEg9fibwQicL8l2ExKOZZTmAHXabQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;caret-color: rgba(0, 0, 0, 0);\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);text-align: left;\">\n<section 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top;\">\n<section style=\"margin-top: -1em;padding: 3px 10px;outline: 0px;display: inline-block;vertical-align: top;color: rgb(0, 154, 68);\">\n\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;\">\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" rel=\"noopener\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/a> <\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" rel=\"noopener\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/a><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491909&idx=1&sn=902e34bcf53e605fba2b457ec6a33e36&chksm=cf7f7180f808f8961542f458a49da14e85d8831449fb65fbf2623d55affc564686a5422f56fd&scene=21#wechat_redirect\" textvalue=\"3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" rel=\"noopener\">3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8<\/a><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 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rel=\"noopener\">http:\/\/mp.weixin.qq.com\/s?__biz=MzkxODUxNDgzNA==&mid=2247487257&idx=1&sn=b2b6ff08473e3b13c54b8459d880d248<\/a><\/p>\n<\/div>\n\u5c0f\u679c\u7684\u5355\u7ec6\u80de\u5206\u6790\u4fdd\u59c6\u7ea7\u6559\u7a0b\uff01\u624b\u628a\u624b\u6559\u4f60\u5bf9\u5355\u7ec6\u80de\u6570\u636e\u4e0b\u8f7d\u548c\u5904\u7406\u5230\u5355\u7ec6\u80de\u6ce8\u91ca\uff01\uff01<\/span><\/p>\n\u6700\u8fd1\u5355\u7ec6\u80de\u7684\u6570\u636e\u5206\u6790\u53c8\u6084\u6084\u7684\u706b\u4e86\u8d77\u6765\uff0c\u5c0f\u4f19\u4f34\u4e5f\u4e86\u89e3\u4e86\u4e0d\u5c11\uff0c\u4f46\u662f\u4e0d\u77e5\u9053\u4ece\u4f55\u4e0b\u624b\uff0c\u800c\u4e14\u5462\u6709\u5173\u5355\u7ec6\u80de\u6570\u636e\u7684\u4e0b\u8f7d\u4e5f\u662f\u4e00\u5934\u96fe\u6c34\u3002\u5176\u5b9e\u5462\u5355\u7ec6\u80de\u6570\u636e\u5904\u7406\u4e5f\u5e76\u4e0d\u662f\u5f88\u590d\u6742\uff0c\u53ea\u662f\u5165\u95e8\u4e0d\u5bb9\u6613\uff0c\u4e00\u65e6\u4e0a\u624b\u540e\u7eed\u7684\u5206\u6790\u5c31\u5f88\u7b80\u5355\u3002\u8fd8\u6709\u5462\uff0c\u6211\u4eec\u5728\u505a\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u5206\u6790\u65f6\u5019\uff0c\u4ed6\u7684\u96be\u70b9\u4e3b\u8981\u5728\u4e8e\u7ec6\u80de\u7684\u8d28\u91cf\u4e0d\u786e\u5b9a\uff0c\u7ec6\u80de\u7684\u6570\u91cf\u5927\uff0c\u4ece\u5355\u7ec6\u80de\u6d4b\u5e8f\u6280\u672f\u8bde\u751f\u81f3\u4eca\uff0c\u6d4b\u5230\u7684\u7ec6\u80de\u901a\u91cf\u8d8a\u6765\u8d8a\u9ad8\u3002\u6240\u4ee5\u5c0f\u679c\u8fd9\u91cc\u51fa\u4e00\u671f\u6559\u7a0b\u53ef\u4ee5\u6559\u5c0f\u4f19\u4f34\u5165\u95e8\u5355\u7ec6\u80de\uff0c\u8ba9\u6211\u4eec\u4ece\u6570\u636e\u4e0b\u8f7d\u5230\u5904\u7406\uff0c\u5c0f\u4f19\u4f34\u5b66\u4f1a\u4e86\u4e4b\u540e\uff0c\u53ef\u4ee5\u4e86\u89e3\u5355\u7ec6\u80de\u5904\u7406\u7684\u5927\u81f4\u6d41\u7a0b\u3002\u4e0b\u9762\u5c0f\u679c\u5462\uff0c\u5c31\u5e26\u5c0f\u4f19\u4f34\u53bb\u4e0b\u8f7d\u81ea\u5df1\u9700\u8981\u7684\u5355\u7ec6\u80de\u6570\u636e\u8fd8\u6709\u5982\u4f55\u53bb\u5904\u7406\u3002<\/span><\/p>\n\u6211\u4eec\u4e00\u822c\u7684\u5bf9\u5355\u7ec6\u80de\u6570\u636e\u5904\u7406\u5462\uff0c\u80af\u5b9a\u662f\u9700\u8981\u548c\u81ea\u5df1\u505a\u7684\u75be\u75c5\u6216\u8005\u80bf\u7624\u76f8\u5173\u7684\u5355\u7ec6\u80de\u6570\u636e\u96c6\uff0c\u6211\u4eec\u5f80\u5f80\u7528\u7684\u505a\u7684\u4e0b\u8f7d\u65b9\u5f0f\u5c31\u662f\u5728NCBI\u8fd9\u4e2a\u5e73\u53f0\uff0cGEO\u6570\u636e\u5e93\u6536\u96c6\uff0c\u4e0b\u9762\u5c0f\u679c\u5c31\u5e26\u5c0f\u4f19\u4f34\u770b\u770b\u5982\u4f55\u53bb\u4e0b\u8f7d\uff01<\/span><\/p>\n\u6211\u4eec\u4e00\u822c\u6253\u5f00\u8fd9\u4e2a\u7f51\u5740\uff1a<\/span>https:\/\/www.ncbi.nlm.nih.gov\/<\/span><\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014755\" data-ratio=\"0.24351851851851852\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_MT3apibaLcULmicticv7I56yHtsywQ.png?resize=553%2C134\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGQHA5RAyESZib8N6K107BSWIQgtDZMT3apibaLcULmicticv7I56yHtsywQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"134 .739990234375\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/span><\/p>\n\u5728\u8fd9\u91cc\u8f93\u5165\u6211\u4eec\u7684\u76f8\u5173\u7684\u4fe1\u606f\u641c\u7d22\uff0c\u8fd9\u91cc\u5c0f\u56fe\u8bd5\u9a8c\u80ba\u764c\u8fdb\u884c\u5c55\u793a\uff0clung cancer \u6211\u4eec\u5728\u641c\u7d22\u7684\u65f6\u5019\u540e\u9762\u8981\u52a0\u5165\u5355\u7ec6\u80de\u76f8\u5173\u7684\u5173\u952e\u5b57\uff0clung caner scRNAseq \u7136\u540e\u5728\u52a0\u4e0a human\u4eba\u7c7b\uff0c\u4e00\u822c\u5c31\u4f7f\u7528lung caner scRNAseq human \u5982\u679c\u662f\u5176\u4ed6\u75be\u75c5 \u6362\u4e00\u4e0b\u5c31\u53ef\u4ee5\uff0c\u6bd4\u5982\u5bab\u9888\u764c cervical cancer scRNAseq human<\/span><\/p>\n\u6765\u770b\u770b\u6548\u679c <\/span> <o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014759\" data-ratio=\"0.46111111111111114\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_ibDc1EOSibeicT3VFrnyAdTFbvgtIA.png?resize=553%2C255\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGyStUDY8TMvCIhibkxQ54WfsQZMX3ibDc1EOSibeicT3VFrnyAdTFbvgtIA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"255 .20001220703125\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/span><\/p>\n\u8fd9\u91cc\u9700\u8981\u628a\u641c\u7d22\u6765\u6e90\u9009\u62e9GEO\u6570\u636e\u5e93\uff0c\u7136\u540e\uff1a<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014758\" data-ratio=\"0.8626560726447219\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_s95u7sic8kFdmbibOq25v7U7nIwEyQ.png?resize=553%2C477\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGnsp8dA1yx5p6bVLgHiaSKy4mqSrs95u7sic8kFdmbibOq25v7U7nIwEyQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"881\" height=\"477 .66998291015625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u7136\u540e\u5c31\u4f1a\u6709\u4e0b\u9762\u7684\u754c\u9762\uff0c\u8fd9\u5c31\u9700\u8981\u5c0f\u4f19\u4f34\u53bb\u81ea\u884c\u53bb\u9009\u62e9\uff0c\u6211\u4eec\u968f\u673a\u70b9\u51fb\u67e5\u770b\u4e00\u4e0b<\/span><\/p>\n <o:p> <\/o:p><\/span> <o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014756\" data-ratio=\"0.8270509977827051\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_ug8Jg3OJiccEGsA1YPutFZdXj64H5Q.png?resize=553%2C457\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGib4rJ5Ka8TwkiascxcbOqJKnoTDug8Jg3OJiccEGsA1YPutFZdXj64H5Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"902\" height=\"457 .94000244140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014757\" data-ratio=\"0.7972465581977471\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_kIUzNPwwL7uXnZoNmP9pV4RGZ0pb6g.png?resize=553%2C441\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGsrv6nOqibNW2PKNxic9JdUkMKCkIUzNPwwL7uXnZoNmP9pV4RGZ0pb6g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"799\" height=\"441 .469970703125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span> <o:page><\/o:page><\/p>\n\u8fd9\u91cc\u6709\u5f88\u591a\u5355\u7ec6\u80de\u6570\u636e\uff0c\u5176\u5b9e\u6211\u4eec\u9009\u62e9\u4e0b\u8f7d\u7684\u4e5f\u662f\u6709\u76ee\u7684\u7684\uff0c\u6211\u4eec\u5982\u679c\u9009\u62e9<\/span><img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014762\" data-ratio=\"0.22692307692307692\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_OjRIcJV0tZdIwxibcKes5ERjsVHHKw.png?resize=519%2C117\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGDXE511s2OnexNpREcxIdvSEEPOjRIcJV0tZdIwxibcKes5ERjsVHHKw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"520\" height=\"117 .989990234375\" width=\"519 .9400024414062\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e09\u4e2a\u91cc\u9762\u7684\u4e00\u79cd\uff0c\u70b9\u51fb\u8fdb\u53bb\u4e0b\u8f7d<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014764\" data-ratio=\"0.36561743341404357\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_NIjtQib3eiayYpsVlDeMWadS9FsZJA.png?resize=553%2C202\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGo7avX8gWKL9wIQkS9HsLf2BOayNIjtQib3eiayYpsVlDeMWadS9FsZJA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"826\" height=\"202 .47000122070312\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014760\" data-ratio=\"0.04980340760157274\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_yopfkHSGV16twFXABnRicboVmqtESQ.png?resize=553%2C27\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGEhVRO3jkP9ic8QpCJqCGHA2cTGyopfkHSGV16twFXABnRicboVmqtESQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"763\" height=\"27 .530029296875\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/span><\/p>\n\u4e0b\u8f7d\u8fd9\u4e2a\u5c31\u53ef\u4ee5\uff0c\u8fd9\u79cd\u662fCSV\u683c\u5f0f\u7684\uff0c\u4e0b\u9762\u5c0f\u679c\u4f1a\u6559\u5c0f\u4f19\u4f34\u5bfc\u5165\u6570\u636e\u683c\u5f0f\u7684\u65b9\u5f0f\uff0c\u5176\u5b9e\u6211\u4eec\u4e0b\u8f7d\u6570\u636e\u96c6\u53ef\u80fd\u8fd8\u6709\u5176\u4ed6\u6570\u636e\u683c\u5f0f\uff0c\u4e00\u822c\u6700\u591a\u7684\u5c31\u662fH5\u683c\u5f0f\uff0c\u8fd8\u6709mtx\u683c\u5f0f\uff0c\u8fd9\u91cc\u5c0f\u4f19\u4f34\u4e0d\u7528\u62c5\u5fc3\uff0c\u5c0f\u679c\u90fd\u4f1a\u6559\u5c0f\u4f19\u4f34\uff0c\u6211\u4eec\u6765\u770b\u4e00\u4e2aH5\u683c\u5f0f\u7684\uff0c<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014763\" data-ratio=\"0.23465211459754434\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_UL11RDbvn9E5Bje6kBkzycHQ3iamfA.png?resize=553%2C129\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGz1tHkn8xofPuNqAwXW7Tx9AeUUL11RDbvn9E5Bje6kBkzycHQ3iamfA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"733\" height=\"129 .94000244140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/span><\/p>\n\u4e0b\u8f7d\u8fd8\u89e3\u538b\u540e<\/span><\/p>\n<\/span><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014761\" data-ratio=\"0.18654434250764526\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_aD54fiaERkTXRBWXozbgSPfh3Tu5hg.png?resize=553%2C103\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGShKUJD3v7yflTD4L0Rj88P8ysaD54fiaERkTXRBWXozbgSPfh3Tu5hg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"654\" height=\"103 .27001953125\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/span><\/p>\n\u5c31\u4f1a\u8fd9\u6837\uff0c<\/span> <o:page><\/o:page><\/p>\n\u8fd8\u6709\u4e00\u79cd<\/span><\/p>\n<\/span><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014765\" data-ratio=\"0.08986928104575163\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_RnE1o8KZemsQiaIzqsOficmPickGCQ.png?resize=553%2C49\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGqHCcsFsxfa2mfrW3gFAD6KmfVNFRnE1o8KZemsQiaIzqsOficmPickGCQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"612\" height=\"49 .72999572753906\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u6211\u4eec\u4e0b\u9762\u5bf9\u8fd9\u4e09\u4e2a\u683c\u5f0f\u5bfc\u5165\u8fdb\u884c\u5b66\u4e60\uff0c<\/span> <\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"> <\/o:p><\/span><\/p>\n\u6211\u4eec\u5148\u4eceH5\u683c\u5f0f\u5b66\u4e60\uff0c\u8fd9\u4e2a\u6bd4\u8f83\u7b80\u5355\u4e00\u884c\u4ee3\u7801\u5c31\u5b66\u4f1a\u4e86\uff0c\u8fd9\u91cc\u5c0f\u679c\u968f\u4fbf\u7528\u4e00\u4e2a\u6570\u636e\u96c6\u5c55\u793a\uff0c<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>library(hdf5r)<\/span><\/code><code>library(Seurat)<\/span><\/code><code>library(dplyr)<\/span><\/span><\/code><\/pre>\n<\/section>\n\u6211\u4eec\u8fd8\u8981\u52a0\u8f7d\u4e00\u4e0b\u8fd9\u4e2a\u5305\uff0c\u7528\u6765\u5904\u7406\u6570\u636e\u7684<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code>data_sample<\/span> <- Read10X_h5(\"GSM4983457_NC_filtered_feature_bc_matrix.h5\"<\/span>) #\u5bfc\u5165H5\u683c\u5f0f\u6570\u636e<\/span><\/span><\/code><code>data_seurat <- CreateSeuratObject(data_sample,project = \"data_sample\"<\/span>) #\u540e\u9762\u5c31\u53ef\u4ee5\u8fdb\u884c\u5355\u7ec6\u80de\u5904\u7406\u7684\u6807\u51c6\u6d41\u7a0b\u4e86<\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014768\" data-ratio=\"0.11077844311377245\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_fSDeCAWiamf8z29Z8UvMXnR9brNACA.png?resize=553%2C61\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGyTyp9Dsv65YPrtibtA2LRIM3kvfSDeCAWiamf8z29Z8UvMXnR9brNACA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"668\" height=\"61 .27001953125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u6837\u5c31\u5bfc\u5165\u6210\u529f\u4e86\uff0c<\/span><\/p>\n\u4e0b\u9762\u6211\u4eec\u5bf9mtx\u683c\u5f0f\u8fdb\u884c\u5bfc\u5165\uff0c<\/span><\/p>\n\u800c\u6211\u4eec\u5f80\u5f80\u4e0b\u8f7dmtx\u683c\u5f0f\u7684\u4e00\u822c\u4f1a\u6709\u8fd9\u4e09\u4e2a\u6587\u4ef6<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014767\" data-ratio=\"0.17936507936507937\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/13_VAaWXUDHCeCBHpYZfCSGiaqCVWvpxA.png?resize=553%2C99\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGWrEzmNorq2ucukmCR67WDdcxmVAaWXUDHCeCBHpYZfCSGiaqCVWvpxA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"630\" height=\"99 .27001953125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code># \u8bfb\u53d610x\u6570\u636e\uff0cdata.dir\u53c2\u6570\u6307\u5b9a\u5b58\u653e\u6587\u4ef6\u7684\u8def\u5f84 <\/span><\/span><\/code><code>seurat_data<\/span> <- Read10X(data.dir = \"E:\\JYStudio\\Rtest\\Ztest1\\\u5355\u7ec6\u80de\\\u5355\u7ec6\u80de\u6570\u636e\u5904\u7406\\Tumor\"<\/span>)<\/span><\/code><code>##\u8fd9\u4e2a\u5c0f\u4f19\u4f34\u8981\u81ea\u884c\u8bbe\u7f6e\u5b58\u653e\u8fd9\u4e2a\u6587\u4ef6\u7684\u76ee\u5f55\uff0c<\/span><\/span><\/code><code># \u7136\u540e\u6211\u4eec\u521b\u5efaSeurat\u5bf9\u8c61<\/span><\/span><\/code><code>data_seurat <- CreateSeuratObject(counts = seurat_data,<\/span><\/code><code> project = \"GSM5155196_352\"<\/span>,<\/span><\/code><code> min.features = 200<\/span>,<\/span><\/code><code> min.cells = 3<\/span>)<\/span><\/code><code>\u8fd9\u4e2a\u5c31\u548c\u4e0a\u8ff0\u521b\u5efa\u5bf9\u8c61\uff0c\u540e\u7eed\u5c31\u53ef\u4ee5\u8fdb\u884c\u5355\u7ec6\u80de\u7684\u6807\u51c6\u6d41\u7a0b\u4e86<\/span><\/code><code>\u4f7f\u7528\u8fd9\u4e2a\u4ee3\u7801\u5c31\u53ef\u4ee5\u5bfc\u5165\u6570\u636e\uff0c<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014766\" data-ratio=\"0.11746987951807229\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/14_W9zyn1LMljVCx9Qy2prclk3iaxF8Ww.png?resize=553%2C65\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGG0bSZvTOG1zorJXALbkY2BtMaWW9zyn1LMljVCx9Qy2prclk3iaxF8Ww\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"664\" height=\"65 .0\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u5bfc\u5165\u540e\u5c31\u662f\u4e00\u6837\u7684\uff0c<\/span><\/p>\n\u6700\u540e\u4e00\u79cd\u5c31\u662fcsv\u683c\u5f0f\u7684\uff0c\u5c0f\u4f19\u4f34\u6709\u7684\u4e0b\u8f7d\u53ea\u6709csv\u683c\u5f0f\u7684\u600e\u4e48\u529e\uff0c\u5c0f\u679c\u8fd9\u91cc\u6559\u5927\u5bb6<\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"swift\"><code>##\u7b2c\u4e8c\u79cd<\/span><\/code><code>library(tidyr)<\/span><\/code><code>library(tidyverse)<\/span><\/code><code>library(org.Mm<\/span>.eg.db)<\/span><\/code><code>library(scater)<\/span><\/code><code>library(scran)<\/span><\/code><code>library(pheatmap)<\/span><\/code><code>library(limma) <\/span><\/code><code>library(edgeR)<\/span><\/code><code>library(dynamicTreeCut)<\/span><\/code><code>library(cluster)<\/span><\/code><code>library(monocle)<\/span><\/code><code>aa <- read.csv(\"GSE122340.csv\"<\/span>,row.names = 1<\/span>,header = T<\/span>)<\/span><\/code><code>all.counts <- as<\/span>.matrix(aa)<\/span><\/code><code>pbmc <- CreateSeuratObject<\/span>(counts = all.counts, project = \"TCGA\"<\/span>, names.delim = \"-\"<\/span>,<\/span><\/code><code> min<\/span>.cells = 3<\/span>, min<\/span>.features = 200<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n\u6211\u4eec\u628a\u5305\u52a0\u8f7d\u597d\u4e86\u4e4b\u540e\uff0c\u7136\u540e\u5bfc\u5165\u81ea\u5df1csv\u683c\u5f0f\u6587\u4ef6\uff0c\u7136\u540e\u5bfc\u5165\u6210\u529f\u540e\uff0c\u5c31\u53ef\u4ee5\u6b63\u5e38\u8fdb\u884c\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u4e86\uff01<\/span><\/p>\n\u597d\u4e86\u5173\u4e8e\u6570\u636e\u4e0b\u8f7d\u548c\u5bfc\u5165\u5c31\u5b8c\u6210\u4e86\uff0c\u5c0f\u4f19\u4f34\u5feb\u53bb\u52a8\u624b\u8bd5\u8bd5\uff0c\u4e0b\u9762\u6211\u4eec\u5c31\u5f00\u59cb\u6b63\u5f0f\u5206\u6790\u4e86\uff01<\/span><\/p>\n\u8fd9\u91cc\u5c0f\u679c\u628a\u5bfc\u5165\u6570\u636e\u5904\u7406\u4e00\u4e0b\uff1a<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code># \u67e5\u770bSeurat\u5bf9\u8c61\u7684\u57fa\u672c\u4fe1\u606f<\/span><\/span><\/code><code>data_seurat<\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014769\" data-ratio=\"0.16512345679012347\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/15_YHoZWB8cXBgAWjezOiavwvp7JhWOxQ.png?resize=553%2C91\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGowk2YzvfVIQ4VHK2ibF0DEO60ZYHoZWB8cXBgAWjezOiavwvp7JhWOxQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"648\" height=\"91 .4000244140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e2a\u5c31\u662f\u53ef\u4ee5\u770b\u5230\u6211\u4eec\u5bfc\u5165\u5355\u7ec6\u80de\u6570\u636e\u7684\u603b\u4f53<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"swift\"><code>data_seurat[[\"percent.mt\"<\/span>]]<-PercentageFeatureSet<\/span>(data_seurat,pattern = \"MT-\"<\/span>)<\/span><\/code><code> <\/span><\/code><code>VlnPlot<\/span>(data_seurat,features = c<\/span>(\"nCount_RNA\"<\/span>,\"nFeature_RNA\"<\/span>,\"percent.mt\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014771\" data-ratio=\"0.9801526717557252\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/16_pP8sv9aDHfsHhuPAFM7DULBWjsyalA.png?resize=553%2C542\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGGot9GDxoOOoKmUGrfXboYBNQpP8sv9aDHfsHhuPAFM7DULBWjsyalA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"655\" height=\"542 .739990234375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u6211\u4eec\u4ee5\u4e0d\u540c\u5f62\u5f0f\u5c55\u793a\u770b\u770b\u57fa\u56e0\u6570\u636e\u90a3\u4e9b\u7ec6\u80de\uff0c\u6bd4\u5982\u7ebf\u7c92\u4f53\u7b49<\/span><\/p>\n\u7136\u540e\u6211\u4eec\u8981\u5254\u9664\uff0c<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cpp\"><code>plot1<-FeatureScatter(data_seurat,feature1 = \"nCount_RNA\"<\/span>,feature2 = \"percent.mt\"<\/span>)<\/span><\/code><code>plot2<-FeatureScatter(data_seurat,feature1 = \"nCount_RNA\"<\/span>,feature2 = \"nFeature_RNA\"<\/span>)<\/span><\/code><code>CombinePlots(plots = list<\/span>(plot1,plot2)) <\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014770\" data-ratio=\"0.9909774436090225\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/17_vOH08ZhDC9ACu7tHNPR0ibcGGaZbjg.png?resize=439%2C435\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGg1uUjicxutaqBGXYgff6oFdWibRvOH08ZhDC9ACu7tHNPR0ibcGGaZbjg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"665\" height=\"435 .25\" width=\"439 .2099609375\" data-recalc-dims=\"1\" \/><\/span> <\/span><\/span><\/p>\n\u5254\u9664\u60c5\u51b5\uff0c\u8fd9\u4e2a\u65e0\u5173\u7d27\u8981\uff0c<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>pbmc<-subset(data_seurat,subset = nFeature_RNA>200 & nFeature_RNA<2500 & percent.mt<5)<\/span><\/span><\/code><\/pre>\n<\/section>\n\u8fd9\u4e2a\u6839\u636e\u5c0f\u4f19\u4f34\u81ea\u5df1\u7684\u60c5\u51b5\u53bb\u9009\u62e9\uff0c\u8303\u56f43000\u8fd8\u67095000 \u8fd8\u6709\u6700\u5c0f\u4e3a200\u8fd8\u662f\u591a\u5c11\uff0c\u5c0f\u4f19\u4f34\u53ef\u4ee5\u6839\u636e\u81ea\u8eab\u60c5\u51b5\uff0c<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code>ncol<\/span>(as<\/span>.data<\/span>.frame<\/span>(pbmc<\/span>[[\"RNA\"]<\/span>]@counts<\/span>))<\/span><\/code><code>pbmc<-NormalizeData(pbmc,normalization.method = \"LogNormalize\"<\/span>,scale.factor = 10000<\/span>)#\u5bf9\u6bcf\u4e2a\u7ec6\u80de\u6807\u51c6\u5316.<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014773\" data-ratio=\"0.18343815513626835\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/18_TSCIxmZuhOUdFm06jEzBJxRoXhic6w.png?resize=553%2C101\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGh9Wp33d14ZmLhxMDRahwLqkxxTSCIxmZuhOUdFm06jEzBJxRoXhic6w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"954\" height=\"101 .52996826171875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u6211\u4eec\u5bf9\u6bcf\u4e2a\u7ec6\u80de\u90fd\u8fdb\u884c\u6807\u51c6\u5316\uff0c<\/span> <o:page><\/o:page><\/p>\n\u4e0b\u9762\u6211\u4eec\u5bf9\u6570\u636e\u8fdb\u884c\u5904\u7406<\/span><\/p>\n\u8fd9\u6b21\u5c0f\u679c\u7ee7\u7eed\u6559\u5c0f\u4f19\u4f34\u5bf9\u6570\u636e\u5904\u7406\uff0c\u8fd8\u6709\u6211\u4eec\u7684\u91cd\u5934\u620f\u5bf9\u5355\u7ec6\u80de\u6ce8\u91ca\uff0c\u4e0b\u9762\u6211\u4eec\u5f00\u59cb\u5427<\/span><\/p>\n\u4e0a\u6b21\u6211\u4eec\u5904\u7406\u5230\uff0c<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014774\" data-ratio=\"0.1592920353982301\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/19_j2S19L1DBXQ6IBEPAR1GIylKTOdfkA.png?resize=553%2C88\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGQZplUtfQmGyiaicRMBLYTHzLvXj2S19L1DBXQ6IBEPAR1GIylKTOdfkA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1017\" height=\"88 .19999694824219\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u5bf9\u6bcf\u4e2a\u7ec6\u80de\u90fd\u8fdb\u884c\u4e86\u6807\u51c6\u5316\uff0c\u4e0b\u9762\u6211\u4eec\u5c31\u662f\u8be5\u5bfb\u627e\u9ad8\u53d8\u7684\u57fa\u56e0\uff0c\u8fd9\u6837\u624d\u80fd\u627e\u5230\u4e0e\u6211\u4eec\u6574\u4f53\u5355\u7ec6\u80de\u76f8\u5173\u7684\u57fa\u56e0\u4e4b\u95f4\u7684\u5173\u7cfb\uff0c\u6765\u770b\u770b\u5982\u4f55\u5bfb\u627e<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code>#\u5bfb\u627e\u9ad8\u53d8\u57fa\u56e0<\/span><\/span><\/code><code>pbmc<\/span><-FindVariableFeatures(pbmc,selection.method = \"vst\"<\/span>,nfeatures = 2000)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014772\" data-ratio=\"0.23094425483503983\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/20_Jz5QNyp4icBqArPJ3WXj3DhOg6viaQ.png?resize=553%2C127\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGbpx8W2EYg6lYa5MzDYsmWNMS4aJz5QNyp4icBqArPJ3WXj3DhOg6viaQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"879\" height=\"127 .8699951171875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u7136\u540e\u6211\u4eec\u8fdb\u884c<\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code>#PCA<\/span><\/span><\/code><code>top10<\/span><-head(VariableFeatures(pbmc))<\/span><\/code><\/pre>\n<\/section>\n\u8fd9\u884c\u4ee3\u7801\u6211\u4eec\u67e5\u770b\u4e00\u4e0b\u524d\u5341\u4e2a\u7684\u9ad8\u53d8\u57fa\u56e0\uff0c\u53ef\u4ee5\u53d1\u73b0\u6211\u7684\u8fd9\u4e2a\u53ea\u67096\u4e2a\u9ad8\u53d8\u57fa\u56e0\uff0c\u53ef\u80fd\u6211\u9009\u53d6\u7684\u6570\u503c\u6bd4\u8f83\u5c11\uff0c\u4e0d\u8fc7\u4e0d\u5f71\u54cd<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014775\" data-ratio=\"0.08728943338437979\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/21_N5BuVUfqnOyEDoJvF95r1BUeoOXg8Q.png?resize=553%2C48\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGG1ibzZjgrjKZlfFErjqkn27XRibN5BuVUfqnOyEDoJvF95r1BUeoOXg8Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"653\" height=\"48 .27001953125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u6211\u4eec\u7ee7\u7eed\u5f80\u4e0b\u8d70<\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code>plot1<-VariableFeaturePlot(pbmc)<\/span><\/span><\/code><code>plot2<-LabelPoints(plot<\/span> = plot1,points=top10,repel=T)<\/span><\/span><\/code><code>plot1<\/span> <\/span><\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014777\" data-ratio=\"1.0062402496099845\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/22_n8Xqq5lm8ddj9OFibibo6J1iaC1Dzg.png?resize=553%2C557\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGVs1373mvD8iauMbBYsokrw5rr3G9n8Xqq5lm8ddj9OFibibo6J1iaC1Dzg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"641\" height=\"557 .2100219726562\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n <o:p> <\/o:p><\/span><\/p>\nplot2<\/span> <o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014776\" data-ratio=\"1.0404984423676011\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/23_8vZJSeyibHibNicZIrbuVdv5aaZAhw.png?resize=553%2C576\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGbVM1icqoSRGnN72AGTBFicr0xaDgg8vZJSeyibHibNicZIrbuVdv5aaZAhw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"642\" height=\"576 .1400146484375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u628a\u6211\u4eec\u627e\u5230\u7684\u9ad8\u53d8\u57fa\u56e0\u653e\u5728\u706b\u5c71\u56fe\u4e0a\u9762\uff0c\u6211\u4eec\u5bfb\u627e\u9ad8\u53d8\u57fa\u56e0\u4e5f\u662f\u4e3a\u4e86\u5728\u540e\u7eed\u5bfb\u627e\u7ec6\u80de\u4e9a\u7fa4\u7684\u65f6\u5019\u5206\u79bb\u6709\u7528\uff0c\u6211\u4eec\u540e\u7eed\u518d\u8bf4\uff0c\u4e0b\u9762\u6211\u4eec\u5bf9\u57fa\u56e0\u8fdb\u884c\u6807\u51c6\u5316\uff0c\u548c\u4e0a\u9762\u4e0d\u540c\u8fd9\u6b21\u662f\u5bf9\u57fa\u56e0<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code>all<\/span>.genes<-rownames(pbmc)<\/span><\/code><code>pbmc<\/span><-ScaleData(pbmc,features = all<\/span>.genes)#\u6bcf\u79cd\u57fa\u56e0\u5728\u7ec6\u80de\u4e2d\u7684\u6807\u51c6\u5316<\/span><\/code><code>pbmc<\/span> <- ScaleData(pbmc, vars.to.regress = \"percent.mt\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" data-imgfileid=\"100014778\" data-ratio=\"0.21203703703703702\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/24_hdzqWSHDT1mPthCSQEY8HVs2rFqFwA.png?resize=553%2C117\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGN0SLiaC5cw2VdYConnJ9dn55ehdzqWSHDT1mPthCSQEY8HVs2rFqFwA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"117 .5999755859375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span> <o:page><\/o:page><\/p>\n\u8fd9\u91cc\u5206\u6790\u597d\u4e86\uff0c\u6211\u4eec\u5c31\u53ef\u4ee5\u540e\u7eed\u5206\u6790\uff0c\u5bf9\u7ec6\u80de\u8fdb\u884c\u805a\u7c7b\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>pbmc<-RunPCA(pbmc,features<\/span> = VariableFeatures(object<\/span> = pbmc))<\/span><\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014779\" data-ratio=\"0.3206627680311891\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/25_tSdricRsp00R0ViaufOlibno4kEXyg.png?resize=553%2C177\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGvnecDy0I74jDzFutwUib4C6yTClptSdricRsp00R0ViaufOlibno4kEXyg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1026\" height=\"177 .53997802734375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u5728\u505a\u4e00\u4e2aPCA\u5206\u6790\uff0c\u770b\u770b\u4e0d\u540c\u7c7b\u4e4b\u95f4\u7684\u5173\u8054<\/span> <\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"> <\/o:p><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>print<\/span>(pbmc[[\"pca\"<\/span>]],dims=1:5,nfeatures=5)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014782\" data-ratio=\"0.6183986371379898\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/26_ibm2BMicr8BQbDia1YqtbicQtEUSPw.png?resize=553%2C342\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGfAYAwMegicAWwtaEU0J0asuRYkCQWibm2BMicr8BQbDia1YqtbicQtEUSPw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"587\" height=\"342 .469970703125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"javascript\"><code>VizDimLoadings(pbmc,dims = 1<\/span>:2<\/span>,reduction = \"pca\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014784\" data-ratio=\"1.0061919504643964\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/27_jRGKOiakaygCWORpVAodCEDxLFAv0A.png?resize=553%2C557\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGibRDaic61LUU7ibBt0rN0Euib733KjRGKOiakaygCWORpVAodCEDxLFAv0A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"646\" height=\"557 .1400146484375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"javascript\"><code>DimPlot(pbmc,reduction = \"pca\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014780\" data-ratio=\"1.0257234726688103\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/28_WNkCfJMOC39Y4MRiaZaicjsbxq5VdA.png?resize=553%2C568\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGG9ia0pyemViaXTc0jf5jsoc8p4RA0WNkCfJMOC39Y4MRiaZaicjsbxq5VdA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"622\" height=\"568 .010009765625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>DimHeatmap(pbmc,dims=1,cells=500,balanced = T)<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014781\" data-ratio=\"1.0207336523125996\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/29_8WQj8Rfvp6cRN8JvibPJo2RboWGibg.png?resize=553%2C565\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGFES4CyPibsLsdFzWlib5TibZ7AM1P8WQj8Rfvp6cRN8JvibPJo2RboWGibg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"627\" height=\"565 .2100219726562\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/p>\n\u8fd9\u4e2a\u5c31\u53ef\u4ee5\u770b\u5230\u6bcf\u4e2a\u57fa\u56e0\u5728\u4e0d\u540c\u7684\u4e9a\u7fa4\u4e4b\u95f4\u7684\u8868\u8fbe\u5982\u4f55\uff0c\u8fd8\u53ef\u4ee5\u5206\u5f00\u6765\u770b<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>DimHeatmap(pbmc,dims = 1:15,cells=500,balanced = T)<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014783\" data-ratio=\"1.0234375\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/30_f6qeE4nwsj8wqNibGt0qKZIhVlUdGA.png?resize=553%2C566\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGv1OfDElic5pd3zOjo7nt7OnWf6f6qeE4nwsj8wqNibGt0qKZIhVlUdGA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"640\" height=\"566 .739990234375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e2a\u5c31\u53ef\u4ee5\u770b\u51fa\u6765\u4e00\u4e9b\u57fa\u56e0\u5728\u4e0d\u540c\u4e9a\u7fa4\u7684\u8868\u8fbe\u91cf\uff0c\u4f46\u662f\u6211\u4eec\u4e0d\u77e5\u9053\u5206\u591a\u5c11\u5408\u9002\u5462\uff0c\u8fd9\u91cc\u5c31\u9700\u8981\u8fdb\u884c\u540e\u7eed\u7684\u5206\u6790\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>pbmc<-JackStraw(pbmc,num.replicate<\/span> = 100)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014785\" data-ratio=\"0.1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/31_SmtbF6HXiazyAicVmouAjpEic0Uibg.png?resize=553%2C55\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGN1cUYHe21Frq6ddw4XfuibqJKdeOYSmtbF6HXiazyAicVmouAjpEic0Uibg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"760\" height=\"55 .33001708984375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e00\u6b65\u5c31\u662f\u5206\u79bb\u5206\u6790\uff0c\u5c0f\u4f19\u4f34\u8010\u5fc3\u7b49\u5f85\u4e00\u4e0b<\/span><\/p>\n\u5c31\u662f\u5728\u5bfb\u627e\u6700\u4f73\u5206\u7fa4<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code>pbmc<-ScoreJackStraw(pbmc,dims=1:20)<\/span><\/span><\/code><code>JackStrawPlot(pbmc,dim=1:20)<\/span><\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014787\" data-ratio=\"0.12771739130434784\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/32_u0310oJ6rJib6HpjibTCNrOHicjSfg.png?resize=553%2C70\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGcVJFwdQiaBdJJNemSI5ibGOBhnIEtu0310oJ6rJib6HpjibTCNrOHicjSfg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"736\" height=\"70 .66999816894531\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014788\" data-ratio=\"0.9984639016897081\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/33_8ukMaVLp0Ba3wNSxDF0ZaX9hTpfTlw.png?resize=553%2C552\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGNGqCtE7zXdFc5BbmPe8IHZ0ic8ukMaVLp0Ba3wNSxDF0ZaX9hTpfTlw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"651\" height=\"552 .8800048828125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e2a\u5c31\u662f\u4e0d\u540c\u4e9a\u7fa4\u7684P\u503c\u60c5\u51b5\uff0c<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>ElbowPlot(pbmc)<\/span><\/code><\/pre>\n<\/section>\n<span style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;font-family: \u7b49\u7ebf;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;\"> <o:p> <\/o:p><\/span> <\/span><\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014786\" data-ratio=\"0.9818181818181818\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/34_8JdYTM1J9TnCUKTf9R5CIdnmicyXJA.png?resize=442%2C434\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGticSwQ2jibtQeG0u1bwxvI4fJW98JdYTM1J9TnCUKTf9R5CIdnmicyXJA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"660\" height=\"434 .65997314453125\" width=\"442 .7099609375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e2a\u788e\u77f3\u56fe\u6211\u4eec\u5c31\u53ef\u4ee5\u770b\u770b\u5728\u54ea\u91cc\u5e73\u7f13\u6211\u4eec\u5c31\u9009\u62e9\u54ea\u91cc\uff0c\u5c0f\u56fe\u8fd9\u5f20\u56fe\u5c31\u9009\u62e917\u6216\u800515\u90fd\u53ef\u4ee5<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code>pbmc<-FindNeighbors(pbmc,dims=1:17)<\/span><\/span><\/code><code>pbmc<-FindClusters(pbmc,resolution = 1.2)#0.4-1.2\u4e4b\u95f4\uff0c\u6570\u503c\u8d8a\u5927\u5206\u7fa4\u8d8a\u591a<\/span><\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014789\" data-ratio=\"0.41656942823803966\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/35_A4Mdj51eMP5Xxtj9u8lhTkicnUD6cA.png?resize=553%2C230\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGIrAqmMPiagYibrOuIgaUicfr4wfOA4Mdj51eMP5Xxtj9u8lhTkicnUD6cA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"857\" height=\"230 .66998291015625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code>head(Idents(pbmc), 5) <span style=\"color: rgba(0, 0, 0, 0.9);font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: var(--articleFontsize);letter-spacing: 0.034em;text-align: justify;\"> <\/span><span style=\"color: rgba(0, 0, 0, 0.9);font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: var(--articleFontsize);letter-spacing: 0.034em;text-align: justify;\"><\/span><\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014790\" data-ratio=\"0.09537037037037037\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/36_DJOdN37rchHVdm499qazkoKJPlDpzg.png?resize=553%2C52\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGVEXM1c3SnzPh8RzEhDV5rBOjDJOdN37rchHVdm499qazkoKJPlDpzg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"52 .72999572753906\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u53ef\u4ee5\u770b\u51fa\u6765\u6211\u4eec\u5206\u4e869\u4e2a\u4e9a\u7fa4<\/span><\/p>\n\u7136\u540e\u6211\u4eec\u8fdb\u884c\u964d\u7ef4<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>pbmc<-RunUMAP(pbmc,dims=1:17)#\u4e00\u79cd\u964d\u7ef4\u65b9\u6cd5<\/span><\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014791\" data-ratio=\"0.38799414348462663\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/37_cK6fhXf4cz0ia9Pn7iadTiajkjyb2Q.png?resize=553%2C214\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGrDW9ibAcO2pLicNicyURSFvCZia0yib8cK6fhXf4cz0ia9Pn7iadTiajkjyb2Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"683\" height=\"214 .79998779296875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>DimPlot(pbmc,reduction = \"umap\"<\/span>)#umap\u7528\u4e8e\u5927\u578b\u6570\u636e<\/span><\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n\u7136\u540e\u540e\u9762\u6211\u4eec\u5c31\u8981\u8fdb\u884c\u964d\u7ef4\u7ed8\u5236\u56fe\uff0c<\/span> <o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014792\" data-ratio=\"1.0015384615384615\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/38_2SbsbVZcJrNlzEknzAA98tGso7OEjA.png?resize=553%2C554\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGjkJj4LicBmCawXCSH2mCzdiaH22SbsbVZcJrNlzEknzAA98tGso7OEjA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"650\" height=\"554 .6099853515625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u7528\u7684\u662fumap\u65b9\u5f0f\u8fdb\u884c\u53ef\u89c6\u5316\u964d\u7ef4\u7ed3\u679c\uff0c\u5bf9\u4e8e\u5927\u6570\u636e\u6765\u8bf4\u662f\u6bd4\u8f83\u9002\u7528\u7684\uff0c\u6211\u4eec\u8fd8\u53ef\u4ee5\u8fdb\u884c\u5176\u4ed6\u65b9\u5f0f\uff0c\u6bd4\u5982tsne\uff0c\u9002\u5408\u5c0f\u6570\u636e<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>pbmc<-RunTSNE(pbmc,dims=1:17)#\u53e6\u4e00\u79cd\u964d\u7ef4\u65b9\u6cd5<\/span><\/span><\/code><code>DimPlot(pbmc,reduction = \"tsne\"<\/span>)#tsne\u7528\u4e8e\u5c0f\u578b\u6570\u636e\uff0c\u5206\u79bb\u6548\u679c\u66f4\u597d<\/span><\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014793\" data-ratio=\"1.0031446540880504\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/39_BicCjysrVZ9LS2KtLqPq6ORI8vr8YA.png?resize=553%2C555\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGIjsmeiapOh5zprbeUwIdk9jAEeBicCjysrVZ9LS2KtLqPq6ORI8vr8YA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"636\" height=\"555 .47998046875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u4e2a\u5c31\u53ef\u4ee5\u770b\u51fa\u6765\uff0c\u5c0f\u679c\u7684\u6570\u636e\u662f\u5c0f\u578b\u6570\u636e\uff0c\u6240\u4ee5\u5462\uff0c\u5728\u4f7f\u7528tsne\u65b9\u5f0f\u7684\u66f4\u660e\u663e\u7684\u5206\u79bb\u5355\u7ec6\u80de\u4e9a\u7fa4<\/span><\/p>\n\u6700\u540e\u5462\u5c31\u662f\u9700\u8981\u627e\u5230\u6211\u4eec\u7684\u5404\u7c7b\u7684\u5173\u952e\u57fa\u56e0<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code>#\u627e\u5404\u7c7b\u5173\u952e\u57fa\u56e0<\/span><\/code><code>pbmc.markers<-FindAllMarkers(pbmc,only.pos<\/span> = T,min.pct<\/span> = 0.25,logfc.threshold<\/span> = 0.25)#\u5bfb\u627e\u6240\u6709\u7c7b\u7684makers\u57fa\u56e0<\/span> <\/span><\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014794\" data-ratio=\"0.3141509433962264\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/40_dtX5AH8WWRxfT5MWyvkIsV319KZFdg.png?resize=553%2C173\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGw2QIRWWFG2dIsga7kmM2Bsn1dtX5AH8WWRxfT5MWyvkIsV319KZFdg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1060\" height=\"173 .94000244140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code>pbmc.markers%>%group_by(cluster) %>% top_n(n=2<\/span>,wt=avg_log2FC)<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014798\" data-ratio=\"0.4679334916864608\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/41_FvkzfwrndScaeB9yDZLVzaTWojwiaQ.png?resize=553%2C259\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGQMFme4gxjsLuzZPrB83BWib9iaKFvkzfwrndScaeB9yDZLVzaTWojwiaQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"842\" height=\"259 .1399841308594\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u5bf9\u7ed3\u679c\u5c55\u793a\uff0c\u4e00\u4e9b\u5173\u952e\u57fa\u56e0\uff0c\u6211\u4eec\u53ef\u4ee5\u9009\u62e9\u524d\u5341\u540d\u7684\u5c55\u793a\u5176\u5728\u5355\u7ec6\u80de\u4e9a\u7fa4\u4e2d\u7684\u5f62\u5f0f<\/span> <\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: var(--articleFontsize);letter-spacing: 0.034em;\"> <\/o:p><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>VlnPlot(pbmc,features = c(\"GPX2\"<\/span>,\"AKR1C2\"<\/span>),slot = \"counts\"<\/span>,log<\/span>=T)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n\u5c0f\u56fe\u8fd9\u91cc\u5bf9\u524d\u4e24\u540d\u7684\u57fa\u56e0\u8fdb\u884c\u5c55\u793a<\/span> <o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014795\" data-ratio=\"0.9984375\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/42_PyjwmPSboYJ0UDnFErlqPCeibxFbag.png?resize=553%2C552\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGFmYM0QFjzRSN40o0XpkeTDzW8PyjwmPSboYJ0UDnFErlqPCeibxFbag\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"640\" height=\"552 .8800048828125\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"swift\"><code>FeaturePlot<\/span>(pbmc,features = c<\/span>(\"GPX2\"<\/span>),reduction = \"tsne\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014796\" data-ratio=\"1.0063492063492063\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/43_I6S6nUhnb65icZx6pYm4boSt4LK59w.png?resize=421%2C424\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGHD6VgHST6ibB7wicmCmHwZsJRMBI6S6nUhnb65icZx6pYm4boSt4LK59w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"630\" height=\"424 .6400146484375\" width=\"421 .95001220703125\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u5c31\u53ef\u4ee5\u5f88\u660e\u663e\u7684\u770b\u51faGPX2\u8fd9\u4e2a\u57fa\u56e0\u5728\u90a3\u4e9b\u7ec6\u80de\u4e2d\u5360\u6bd4\u6700\u591a\uff0c<\/span><\/p>\nreduction = “tsne”\u8fd9\u4e2a\u53c2\u6570\u53ef\u4ee5\u6539\u53d8\u7ed8\u56fe\u7684\u65b9\u5f0f\uff0c<\/span><\/p>\n\u7136\u540e\u6211\u4eec\u5bf9\u524d\u5341\u540d\u7684\u8fdb\u884c\u4fdd\u5b58<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code>top10<\/span><-pbmc.markers%>%group_by(cluster) %>% top_n(n=10<\/span>,wt=avg_log2FC)<\/span><\/code><code>DoHeatmap(pbmc,features = top10<\/span>$gene)+NoLegend()<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014799\" data-ratio=\"1.0156985871271587\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/44_8m6UrZrIyuuL1DBBONENFg0nuMNe3A.png?resize=553%2C562\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGp7snxbeAjfVTDTvToYiaV6XVd8m6UrZrIyuuL1DBBONENFg0nuMNe3A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"637\" height=\"562 .4099731445312\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u5bf9\u5173\u952e\u57fa\u56e0\u8868\u8fbe\u91cf\u8fdb\u884c\u70ed\u56fe\u7684\u7ed8\u5236\uff0c\u770b\u770b\u8fd9\u4e9b\u57fa\u56e0\u5728\u90a3\u4e2a\u4e9a\u7fa4\u4e2d\u8868\u8fbe\u5982\u4f55\uff0c<\/span><\/p>\nsaveRDS(pbmc,”pbmc.rds”)<\/span><\/p>\n\u6700\u540e\u6211\u4eec\u4fdd\u5b58\u4e00\u4e0b\u8fd9\u4e9b\u4fe1\u606f\uff0c<\/span><\/p>\n\u7136\u540e\u6211\u4eec\u5bf9\u8fd9\u4e9b\u7ec6\u80de\u8fdb\u884c\u6ce8\u91ca\uff0c\u8fd9\u91cc\u6211\u4eec\u4f7f\u7528SingleR\u5305\u8fdb\u884c\u81ea\u52a8\u6ce8\u91ca\uff0c\u6bd4\u8f83\u65b9\u4fbf\uff0c\u5c0f\u679c\u4ee5\u53ca\u628a\u8fd9\u4e2a\u6570\u636e\u7684\u4fe1\u606f\u4e0b\u8f7d\u4e0b\u6765\u4e86\uff0c\u5c0f\u4f19\u4f34\u53ef\u4ee5\u81ea\u884c\u53bb\u540e\u53f0\u9886\u53d6<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code>library(SingleR)<\/span><\/code><code>#<\/span>BiocManager::install(\"celldex\"<\/span>)<\/span><\/code><code>library(celldex) <\/span><\/code><code>library(Seurat)<\/span><\/code><code>library(pheatmap)<\/span><\/code><\/pre>\n<\/section>\n\u52a0\u8f7d\u4e00\u4e0b\u5305\uff0c\u6211\u4eec\u8fdb\u884c\u5355\u7ec6\u80de\u6ce8\u91ca<\/span><\/p>\n##\u4e0b\u8f7d\u6ce8\u91ca\u6570\u636e\u5e93\uff0c\u8fd9\u91cc\u627e\u5c0f\u679c\u9886\u53d6\u4e0e\u559c\u7231\u4e0b\u8f7d\u597d\u7684\u6570\u636e\u5e93\u4fe1\u606f<\/span><\/p>\n\u5c0f\u4f19\u4f34\u4e5f\u53ef\u4ee5\u81ea\u884c\u53bb\u4e0b\u8f7d\uff0c\u4e0d\u8fc7\u4e00\u822c\u4f1a\u56e0\u4e3a\u7f51\u7edc\u95ee\u9898\u5931\u8d25\uff0c<\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code>hpca<\/span>.se<\/span> <-<\/span> HumanPrimaryCellAtlasData<\/span>()<\/span><\/code><code>hpca<\/span>.se<\/span><\/span><\/code><code> <\/span><\/code><code> <\/span><\/code><code>#\u76f4\u63a5load<\/span>\u4e0b\u8f7d\u597d\u7684\u6570\u636e\u5e93<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code>load<\/span>(\"HumanPrimaryCellAtlas_hpca<\/span>.se_human<\/span>.RData<\/span>\")<\/span><\/code><code> <\/span><\/code><code>load<\/span>(\"BlueprintEncode_bpe<\/span>.se_human<\/span>.RData<\/span>\")<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014797\" data-ratio=\"0.10063897763578275\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/45_cgvLsRrUGqp68nebfHrADeV3rzO4VQ.png?resize=553%2C55\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGpgH6l4NdKJLVa7iaPiaIpvXETPcgvLsRrUGqp68nebfHrADeV3rzO4VQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"626\" height=\"55 .66998291015625\" width=\"553 .739990234375\" data-recalc-dims=\"1\"> <\/span><o:p style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;\"> <\/o:p><\/span><\/p>\npbmc#\u4e0a\u9762\u5c0f\u679c\u5904\u7406\u597d\u7684\u5355\u7ec6\u80de\u4fe1\u606f<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014800\" data-ratio=\"0.17565485362095531\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/46_9ozS4MXjwDLfLiaoDuHNYAguG27lGA.png?resize=553%2C97\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGS1icULtuBicuKMP5SJlGZQdXV2s9ozS4MXjwDLfLiaoDuHNYAguG27lGA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"649\" height=\"97 .20001220703125\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code>meta=pbmc@meta.data #pbmc\u7684meta\u6587\u4ef6\uff0c\u5305\u542b\u4e86seurat\u7684\u805a\u7c7b\u7ed3\u679c <\/span><\/span><\/code><code>head(meta)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014802\" data-ratio=\"0.3614931237721022\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/47_q1YJmXNRGnwHiawvibNZxXzhvKic1Q.png?resize=553%2C200\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGlTtMOOH9QMiaJzNEZWb5xqoPLzQbq1YJmXNRGnwHiawvibNZxXzhvKic1Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1018\" height=\"200 .1400146484375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u67e5\u770b\u4e00\u4e0b\u4fe1\u606f<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code>plot1 <- DimPlot(pbmc, reduction = \"umap\"<\/span>, label = TRUE<\/span>)<\/span><\/code><code> <\/span><\/code><code>plot2<-DimPlot(pbmc, reduction = \"tsne\"<\/span>,<\/span><\/code><code> label = TRUE<\/span>)<\/span><\/code><code>plot1 + plot2<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014803\" data-ratio=\"0.7731481481481481\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/48_hk4O6GcVWA9nQ5nZaicKE4XbNibXxw.png?resize=553%2C427\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGG0M27NtnyogjJ93GRvrmnlCibHgPhk4O6GcVWA9nQ5nZaicKE4XbNibXxw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"427 .94000244140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u7136\u540e\u6211\u4eec\u5c31\u5f00\u59cb\u6ce8\u91ca\u5427\uff01<\/span><\/p>\n <o:p> <\/o:p><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>#\u8fdb\u884csingleR\u6ce8\u91ca<\/span><\/span><\/code><code>pbmc_for_SingleR <- GetAssayData(pbmc, slot=\"data\"<\/span>) ##\u83b7\u53d6\u6807\u51c6\u5316\u77e9\u9635<\/span><\/span><\/code><code>pbmc.hesc <- SingleR(test<\/span> = pbmc_for_SingleR, ref = hpca.se, labels = hpca.se$label<\/span>.main) #<\/span><\/span><\/code><code>pbmc.hesc<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014804\" data-ratio=\"0.37648327939590076\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/49_MhHTwlQyT8q1BoTaMr7ic4vfBWhxlw.png?resize=553%2C208\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGz1pz562CM6h27FsEkYkVH4p9FMhHTwlQyT8q1BoTaMr7ic4vfBWhxlw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"927\" height=\"208 .47000122070312\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>#seurat \u548c singleR\u7684table\u8868<\/span><\/span><\/code><code>table(pbmc.hesc$labels<\/span>,meta$seurat_clusters<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<\/span><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014801\" data-ratio=\"0.42219020172910665\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/50_LmSzuSpDNLzbEX57Y5mpVrE0ChJE1A.png?resize=553%2C233\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGZNLLuvMCicwaKSyRw9X0VK3odLmSzuSpDNLzbEX57Y5mpVrE0ChJE1A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"694\" height=\"233 .79998779296875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u6ce8\u91ca\u7ed3\u679c<\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code>#umap\u56fe<\/span><\/span><\/code><code> <\/span><\/code><code>pbmc@meta.data$labels <-pbmc.hesc$labels<\/span><\/code><code> <\/span><\/code><code> <\/span><\/code><code>print<\/span>(DimPlot(pbmc, group.by = c(\"seurat_clusters\"<\/span>, \"labels\"<\/span>),reduction = \"umap\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014809\" data-ratio=\"0.7675925925925926\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/51_ia1Micpia4WMSDUWz4ic1Ixu3VWB1A.png?resize=553%2C425\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGlcpOyJnP5TpZcVxeccic9a9vMqKWPia1Micpia4WMSDUWz4ic1Ixu3VWB1A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"425 .1400146484375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u8fd9\u91cc\u6211\u4eec\u6ce8\u91ca\u5b8c\u6210\u4e86\uff01<\/span><\/p>\n\u4f46\u662f\u6211\u4eec\u8fd8\u9700\u8981\u591a\u4e2a\u6570\u636e\u5e93\u9a8c\u8bc1\u4ee5\u53ca\u5bf9\u7ed3\u679c\u8bca\u65ad\uff0c\u6765\u770b\u770b\uff1a<\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code>##\u591a\u4e2a\u6570\u636e\u5e93\u6ce8\u91ca<\/span><\/span><\/code><code>pbmc3 <- pbmc<\/span><\/code><code> <\/span><\/code><code>pbmc3.hesc <- SingleR(test = pbmc_for_SingleR, ref = list<\/span>(BP=bpe.se, HPCA=hpca.se),<\/span><\/code><code> labels = list<\/span>(bpe.se$label.main, hpca.se$label.main))<\/span><\/code><code>table(pbmc3.hesc$labels,meta$seurat_clusters)<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014807\" data-ratio=\"0.4830028328611898\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/52_apfF83IxmFfSanrPAPTtrxAxXgFcUg.png?resize=553%2C267\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGfiavUkEl3f7sDyVlvhKiabUTbMiapfF83IxmFfSanrPAPTtrxAxXgFcUg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"706\" height=\"267 .4700012207031\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code>pbmc3@meta.data$labels <-pbmc3.hesc$labels<\/span><\/code><code> <\/span><\/code><\/pre>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code>print(DimPlot(pbmc3, group<\/span>.by<\/span> = c(\"seurat_clusters\"<\/span>, \"labels\"<\/span>),reduction = \"tsne\"<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014806\" data-ratio=\"1.0093457943925233\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/53_FEVJ4ibvQVfEicqFQnPrP2VpYEAf4w.png?resize=553%2C558\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGQ6jic16gt1njLvWjTx9icLS5Y8ib4FEVJ4ibvQVfEicqFQnPrP2VpYEAf4w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"642\" height=\"558 .93994140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code>#\u7ed3\u679c\u8bca\u65ad<\/span><\/span><\/code><code>print<\/span>(plotScoreHeatmap(pbmc.hesc))<\/span><\/code><code> <\/span><\/code><code>plotDeltaDistribution(pbmc.hesc, ncol = 3)<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014808\" data-ratio=\"0.7861111111111111\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/54_JdnAnlHXu2F0CslH0vNXBrfMHicS5g.png?resize=553%2C435\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGSQD1SF51d3arYeMKVVGhGQIMicJdnAnlHXu2F0CslH0vNXBrfMHicS5g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"435 .34002685546875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code>#cluster\u7ed3\u679c\u5bf9\u6bd4<\/span><\/span><\/code><code> <\/span><\/code><code>tab<\/span> <- table(label = pbmc.hesc$labels<\/span>,<\/span><\/code><code> <\/span><\/code><code> cluster = meta$seurat_clusters<\/span>)<\/span><\/code><code> <\/span><\/code><code>pheatmap(log10(tab + 10<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<o:page><\/o:page><\/p>\n<img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014805\" data-ratio=\"0.9863429438543247\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/55_cmcubZuSw3WM9ic1WH6RFjicWTPiaA.png?resize=553%2C546\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwoicvOBiafjVyOT0qf8C9eGGdLRFLruwOCt9CfzaqwePFIbEvQkicmcubZuSw3WM9ic1WH6RFjicWTPiaA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"659\" height=\"546 .2100219726562\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/span><\/p>\n\u597d\u4e86\uff0c\u5230\u6b64\u6211\u4eec\u7684\u5355\u7ec6\u80de\u5165\u95e8\u5b66\u4e60\u7684\u6559\u7a0b\u5c31\u7ed3\u675f\u4e86\uff0c\u5c0f\u4f19\u4f34\u8981\u597d\u597d\u6d88\u5316\u4e00\u4e0b\uff0c\u5bf9\u6b64\u6570\u636e\u8fdb\u884c\u4eb2\u624b\u64cd\u4f5c\uff01<\/span><\/p>\n <\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100014811\" data-ratio=\"0.5555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/56_VmRkv7tSNKliafDnsuFR7BoeDgBrww.jpg?w=640\" 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justify;\"><\/span><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100014812\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/57_FbEg9fibwQicL8l2ExKOZZTmAHXabQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAx3HetBkB7aOGibFWKqibqIATyC4zFibzCgdrxFwRep6cDMLIbA0FbEg9fibwQicL8l2ExKOZZTmAHXabQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;caret-color: rgba(0, 0, 0, 0);\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);text-align: left;\">\n<section style=\"outline: 0px;font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;outline: 0px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;outline: 0px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;outline: 0px;\">\n<section style=\"outline: 0px;display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;outline: 0px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;outline: 0px;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"margin-top: -1em;padding: 3px 10px;outline: 0px;display: inline-block;vertical-align: top;color: rgb(0, 154, 68);\">\n\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;\">\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 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rel=\"noopener\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/a><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491909&idx=1&sn=902e34bcf53e605fba2b457ec6a33e36&chksm=cf7f7180f808f8961542f458a49da14e85d8831449fb65fbf2623d55affc564686a5422f56fd&scene=21#wechat_redirect\" textvalue=\"3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" rel=\"noopener\">3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8<\/a><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491905&idx=1&sn=fb34ebd6282962c7803badaee7a39096&chksm=cf7f7184f808f8929608c0050344ad86193d0fc6455c1c18e16cc17bf4b37832b63d3b18284e&scene=21#wechat_redirect\" textvalue=\"4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;\" rel=\"noopener\">4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b<\/span><\/a><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493633&idx=1&sn=2263dfe7572c0bcf6816c2c1a37d79de&chksm=cf7f78c4f808f1d2209321d6ace39fde5fa60c0691b56f88eac21916f5a2872ce8ee345f1d5f&scene=21#wechat_redirect\" textvalue=\"\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;letter-spacing: 0.578px;font-size: 14px;\" rel=\"noopener\">5.\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01<\/a><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n <o:p> <\/o:p><\/span> 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[…]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58633"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=58633"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58633\/revisions"}],"predecessor-version":[{"id":58691,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58633\/revisions\/58691"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=58633"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=58633"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=58633"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}