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15px;\">\u6b63\u662f\u5728\u8fd9\u79cd\u80cc\u666f\u4e0b\uff0c”scran”\u5305\u5d2d\u9732\u5934\u89d2\uff0c\u6210\u4e3a\u5904\u7406\u548c\u89e3\u91ca\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u7684\u4e0d\u53ef\u6216\u7f3a\u7684\u5de5\u5177\u3002\u8fd9\u4e2a\u5305\u5728\u6570\u636e\u5904\u7406\u65b9\u9762\u8868\u73b0\u51fa\u8272\uff0c\u901a\u8fc7\u652f\u6301\u9ad8\u7ea7\u7684\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u9884\u5904\u7406\uff0c\u4f8b\u5982\u8fc7\u6ee4\u4f4e\u8868\u8fbe\u57fa\u56e0\u548c\u6821\u6b63\u6280\u672f\u504f\u5dee\uff0c\u6709\u52a9\u4e8e\u51cf\u5c11\u6570\u636e\u4e2d\u7684\u566a\u58f0\uff0c\u63d0\u9ad8\u6570\u636e\u8d28\u91cf\u3002\u8fd9\u4f7f\u5f97\u540e\u7eed\u7684\u5206\u6790\u66f4\u51c6\u786e\uff0c\u66f4\u5177\u6709\u53ef\u89e3\u91ca\u6027\uff0c\u4e3a\u7ec6\u80de\u5185\u90e8\u7684\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u63d0\u4f9b\u4e86\u66f4\u6e05\u6670\u7684\u56fe\u666f\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;font-size: 15px;\">“scran”\u7684\u5185\u5efa\u964d\u7ef4\u6280\u672f\u5982PCA\u548ct-SNE\uff0c\u5145\u5206\u5229\u7528\u4e86\u6570\u5b66\u548c\u7edf\u8ba1\u5b66\u7684\u539f\u7406\uff0c\u5c06\u9ad8\u7ef4\u7684\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u8f6c\u5316\u4e3a\u4e8c\u7ef4\u6216\u4e09\u7ef4\u7684\u8868\u793a\u3002\u8fd9\u4e2a\u964d\u7ef4\u8fc7\u7a0b\u4e0d\u4ec5\u4fbf\u4e8e\u6570\u636e\u7684\u53ef\u89c6\u5316\uff0c\u4e5f\u4e3a\u805a\u7c7b\u548c\u7ec6\u80de\u4e9a\u578b\u7684\u5212\u5206\u63d0\u4f9b\u4e86\u57fa\u7840\u3002\u800c\u805a\u7c7b\u529f\u80fd\uff0c\u6b63\u662f”scran”\u5305\u7684\u53c8\u4e00\u4eae\u70b9\u3002\u5b83\u53ef\u4ee5\u5c06\u8868\u8fbe\u6a21\u5f0f\u76f8\u4f3c\u7684\u7ec6\u80de\u5f52\u4e3a\u4e00\u7c7b\uff0c\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u53d1\u73b0\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u548c\u72b6\u6001\u3002\u6b64\u5916\uff0c\u5bf9\u6279\u6b21\u6548\u5e94\u7684\u6821\u6b63\u529f\u80fd\u4e5f\u4f7f\u5f97\u6570\u636e\u66f4\u5177\u53ef\u6bd4\u6027\uff0c\u4e3a\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u7684\u5dee\u5f02\u63d0\u4f9b\u4e86\u66f4\u51c6\u786e\u7684\u8bc4\u4f30\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;font-size: 15px;\"><em style=\"font-family: \u5b8b\u4f53;font-size: 16px;letter-spacing: 0.578px;text-align: left;white-space: normal;color: rgb(178, 178, 178);\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.476px;text-decoration: underline;\">\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c111\u201d\u9886\u53d6\u4ee3\u7801\uff0c\u4ee3\u7801\u7f16\u53f7\uff1a231105<\/span><\/em><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;text-indent: 0pt;letter-spacing: 0.034em;font-family: \u5b8b\u4f53;font-variant-numeric: normal;font-variant-east-asian: normal;\">\u4e5f\u5c31\u662f\u8bf4\uff0c”scran”\u5305\u4e0d\u4ec5\u586b\u8865\u4e86\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u5206\u6790\u5de5\u5177\u7684\u7a7a\u767d\uff0c\u66f4\u6210\u4e3a\u751f\u7269\u5b66\u5bb6\u4eec\u63ed\u793a\u7ec6\u80de\u95f4\u5dee\u5f02\u548c\u529f\u80fd\u7684\u5f97\u529b\u52a9\u624b\u3002\u8fd9\u4e2a\u5305\u7684\u591a\u6837\u529f\u80fd\u548c\u5f3a\u5927\u6027\u80fd\uff0c\u65e0\u7591\u4e3a\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6280\u672f\u7684\u5e94\u7528\u62d3\u5bbd\u4e86\u9053\u8def\uff0c\u63a8\u52a8\u4e86\u751f\u7269\u5b66\u9886\u57df\u7684\u524d\u6cbf\u7814\u7a76\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u8981\u4f7f\u7528<\/span><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;\">scran<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5305\uff0c\u53ef\u4ee5\u5728R\u4e2d\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\u8fdb\u884c\u5b89\u88c5\u548c\u52a0\u8f7d\uff1a<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">> install.packages(\"scran\") #\u5b89\u88c5scran\u8bed\u8a00\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">> library(scran) #\u52a0\u8f7d\u8bed\u8a00\u5305<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u9996\u5148\uff0c<\/span><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;\">“scran”<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5305\u63d0\u4f9b\u4e86\u7528\u4e8e\u6570\u636e\u9884\u5904\u7406\u7684\u51fd\u6570\uff0c\u5982\u53bb\u9664\u4f4e\u8868\u8fbe\u57fa\u56e0\u548c\u9ad8\u5ea6\u53d8\u5f02\u57fa\u56e0\uff0c\u4ece\u800c\u51cf\u5c11\u566a\u58f0\u7684\u5f71\u54cd\uff0c\u63d0\u9ad8\u6570\u636e\u8d28\u91cf\u3002\u63a5\u7740\uff0c<\/span><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;\">“scran”<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5305\u5229\u7528\u4e86\u9ad8\u7ea7\u964d\u7ef4\u6280\u672f\uff0c\u5982\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u548ct\u5206\u5e03\u968f\u673a\u90bb\u57df\u5d4c\u5165\uff08t-SNE\uff09\uff0c\u5c06\u9ad8\u7ef4\u6570\u636e\u6620\u5c04\u5230\u4f4e\u7ef4\u7a7a\u95f4\uff0c\u4f7f\u5f97\u6570\u636e\u66f4\u6613\u4e8e\u53ef\u89c6\u5316\u548c\u5206\u6790\u3002\u8fd9\u6709\u52a9\u4e8e\u7814\u7a76\u4eba\u5458\u53d1\u73b0\u7ec6\u80de\u4e4b\u95f4\u7684\u6a21\u5f0f\u548c\u805a\u7c7b\u3002<\/span><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u53e6\u4e00\u4e2a<\/span><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;\">“scran”<\/span><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5305\u7684\u5173\u952e\u529f\u80fd\u662f\u805a\u7c7b\u5206\u6790\u3002\u5b83\u63d0\u4f9b\u4e86\u591a\u79cd\u805a\u7c7b\u65b9\u6cd5\uff0c\u5982\u5c42\u6b21\u805a\u7c7b\u548cK\u5747\u503c\u805a\u7c7b\uff0c\u53ef\u4ee5\u5c06\u7ec6\u80de\u5206\u6210\u4e0d\u540c\u7684\u7fa4\u96c6\uff0c\u6bcf\u4e2a\u7fa4\u96c6\u4ee3\u8868\u4e00\u4e2a\u7ec6\u80de\u7c7b\u578b\u6216\u72b6\u6001\u3002\u8fd9\u6709\u52a9\u4e8e\u786e\u5b9a\u7ec4\u7ec7\u6216\u5668\u5b98\u4e2d\u4e0d\u540c\u7ec6\u80de\u79cd\u7c7b\u7684\u5b58\u5728\u4ee5\u53ca\u5b83\u4eec\u4e4b\u95f4\u7684\u76f8\u5bf9\u6bd4\u4f8b\u3002<\/span><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 15px;\">\u793a\u4f8b\uff1a<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 15px;\">\u5f53\u4f7f\u7528scran\u8bed\u8a00\u5305\u65f6\uff0c\u4e00\u4e2a\u7b80\u5355\u7684\u793a\u4f8b\u53ef\u4ee5\u662f\u5bf9\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u7684\u9884\u5904\u7406\u3001\u964d\u7ef4\u548c\u805a\u7c7b\u5206\u6790\u3002\u9996\u5148\uff0c\u4f60\u9700\u8981\u5b89\u88c5\u5e76\u52a0\u8f7dscran\u5305\u4ee5\u53ca\u5176\u4ed6\u6240\u9700\u7684\u5305\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">> install.packages(\"scran\") <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">> library(scran)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\"># \u5047\u8bbe\u4f60\u5df2\u7ecf\u6709\u4e00\u4e2a\u540d\u4e3a'sce'\u7684SingleCellExperiment\u5bf9\u8c61<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\"># \u8fd9\u91cc\u5047\u8bbe'sce'\u5bf9\u8c61\u5df2\u7ecf\u5305\u542b\u4e86\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u548c\u6837\u672c\u4fe1\u606f<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 15px;\">\u4f7f\u7528scran\u5305\u7684\u51fd\u6570\u5bf9\u6570\u636e\u8fdb\u884c\u9884\u5904\u7406\uff0c\u6bd4\u5982\u53bb\u9664\u4f4e\u8868\u8fbe\u57fa\u56e0\u548c\u9ad8\u5ea6\u53d8\u5f02\u57fa\u56e0\uff0c\u4ee5\u53ca\u5f52\u4e00\u5316\u5904\u7406\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\"># \u8fc7\u6ee4\u4f4e\u8868\u8fbe\u57fa\u56e0\u548c\u9ad8\u5ea6\u53d8\u5f02\u57fa\u56e0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">> sce <- quickCluster(sce)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\"># \u5f52\u4e00\u5316\u5904\u7406<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">> sce <- computeSumFactors(sce)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">> sce <- logNormCounts(sce)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5229\u7528PCA\uff08\u4e3b\u6210\u5206\u5206\u6790\uff09\u5c06\u9ad8\u7ef4\u6570\u636e\u964d\u7ef4\uff0c\u4ee5\u4fbf\u8fdb\u884c\u53ef\u89c6\u5316\u3002<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\"># \u6267\u884cPCA\u964d\u7ef4<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">> sce <- runPCA(sce)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 15px;\">\u7ed8\u5236PCA\u964d\u7ef4\u540e\u7684\u6570\u636e\u5206\u5e03\uff0c\u53ef\u4ee5\u7528\u6563\u70b9\u56fe\u5c55\u793a\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u6216\u6837\u672c\u4e4b\u95f4\u7684\u5dee\u5f02\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\"># \u7ed8\u5236PCA\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">> plotPCA(sce, colour_by = \"cell_type\")<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.75\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_UejhOxAsrsZDnp7WKcicCq6Hu6GxlQ.png?resize=553%2C415\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6iaDcLL4qTldYXwUXoGNQTVxCrkUejhOxAsrsZDnp7WKcicCq6Hu6GxlQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"800\" height=\"415 .2099609375\" width=\"553 .6099853515625\" data-recalc-dims=\"1\"><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><o:page><\/o:page><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.8703703703703703\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_9Njicu1UD1N1EHSq1UH4HHuPIAvsXQ.png?resize=553%2C481\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6yZP4YjCq3Cpvy8OBqShWVZ0Fr9Njicu1UD1N1EHSq1UH4HHuPIAvsXQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"864\" height=\"481 .80999755859375\" width=\"553 .5399780273438\" data-recalc-dims=\"1\"><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 15px;\">\u4f7f\u7528scran\u5305\u4e2d\u7684\u805a\u7c7b\u51fd\u6570\uff0c\u5c06\u7ec6\u80de\u5206\u6210\u4e0d\u540c\u7684\u7fa4\u96c6\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\"># \u8fdb\u884c\u805a\u7c7b\u5206\u6790\uff0c\u5047\u8bbe\u4f60\u8981\u5206\u4e3a3\u4e2a\u805a\u7c7b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">> sce <- buildSNNGraph(sce, use.dimred = \"PCA\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">> sce <- clusterCells(sce, method = \"igraph\", k = 3)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;\"><span style=\"font-family: \u5b8b\u4f53;text-indent: 0pt;letter-spacing: 0.034em;\">\u7ed8\u5236\u805a\u7c7b\u7ed3\u679c\uff0c\u5c06\u4e0d\u540c\u805a\u7c7b\u7528\u4e0d\u540c\u989c\u8272\u6807\u6ce8\u5728\u964d\u7ef4\u56fe\u4e0a\u3002<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"shell\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\"># \u7ed8\u5236\u5e26\u805a\u7c7b\u6807\u7b7e\u7684PCA\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 15px;\">> plotPCA(sce, colour_by = \"cluster\")     <\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;font-size: 15px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.7681347150259067\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_2A6SsrhOur1GypN5p3eErmIKr50ssw.png?resize=553%2C425\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAz3JeicJYr7pOY6FNYBTEIa6DH0meWfwyo6tmBx03Y6X3z9b2A6SsrhOur1GypN5p3eErmIKr50ssw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"772\" height=\"425 .2099609375\" width=\"553 .5399780273438\" data-recalc-dims=\"1\"><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53;text-indent: 0pt;letter-spacing: 0.034em;\">\u901a\u8fc7\u8fd9\u4e2a\u7b80\u5355\u7684\u793a\u4f8b\uff0c\u53ef\u4ee5\u4f7f\u7528scran\u5305\u5bf9\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u8fdb\u884c\u9884\u5904\u7406\u3001\u964d\u7ef4\u548c\u805a\u7c7b\u5206\u6790\uff0c\u5e76\u5728\u964d\u7ef4\u56fe\u4e0a\u53ef\u89c6\u5316\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u6216\u805a\u7c7b\u7684\u5206\u5e03\u60c5\u51b5\u3002\u8fd9\u6709\u52a9\u4e8e\u66f4\u597d\u5730\u7406\u89e3\u6570\u636e\u4e2d\u7684\u7ec6\u80de\u591a\u6837\u6027\u548c\u5dee\u5f02\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53;text-indent: 0pt;letter-spacing: 0.034em;\">\u6b64\u5916\uff0c”scran”\u5305\u8fd8\u5177\u5907\u5728\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u5206\u6790\u4e2d\u81f3\u5173\u91cd\u8981\u7684\u6279\u6b21\u6548\u5e94\u6821\u6b63\u80fd\u529b\u3002\u5728\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u5b9e\u9a8c\u4e2d\uff0c\u6570\u636e\u53ef\u80fd\u6765\u81ea\u4e0d\u540c\u7684\u5b9e\u9a8c\u6279\u6b21\uff0c\u751a\u81f3\u6765\u81ea\u4e0d\u540c\u7684\u5b9e\u9a8c\u5ba4\u6216\u6d4b\u5e8f\u5e73\u53f0\uff0c\u8fd9\u53ef\u80fd\u5bfc\u81f4\u6280\u672f\u6027\u7684\u504f\u5dee\u3002\u8fd9\u4e9b\u6279\u6b21\u6548\u5e94\u4f1a\u5bf9\u6570\u636e\u7684\u7ed3\u679c\u4ea7\u751f\u663e\u8457\u5f71\u54cd\uff0c\u53ef\u80fd\u63a9\u76d6\u6216\u8bef\u5bfc\u7814\u7a76\u4eba\u5458\u5bf9\u7ec6\u80de\u95f4\u771f\u5b9e\u751f\u7269\u5b66\u5dee\u5f02\u7684\u7406\u89e3\u3002<br \/><\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53;text-indent: 0pt;letter-spacing: 0.034em;\">\u6279\u6b21\u6548\u5e94\u6821\u6b63\u662f\u4e00\u4e2a\u5173\u952e\u6b65\u9aa4\uff0c\u65e8\u5728\u6d88\u9664\u4e0d\u540c\u6279\u6b21\u4e4b\u95f4\u7684\u6280\u672f\u504f\u5dee\uff0c\u4f7f\u5f97\u6570\u636e\u66f4\u5177\u6709\u4e00\u81f4\u6027\u548c\u53ef\u6bd4\u6027\u3002”scran”\u5305\u901a\u8fc7\u63d0\u4f9b\u591a\u79cd\u65b9\u6cd5\uff0c\u8d4b\u4e88\u4e86\u7814\u7a76\u4eba\u5458\u5904\u7406\u6279\u6b21\u6548\u5e94\u7684\u7075\u6d3b\u6027\uff0c\u4ee5\u9002\u5e94\u4e0d\u540c\u6570\u636e\u96c6\u548c\u7814\u7a76\u8bbe\u8ba1\u7684\u9700\u6c42\u3002\u8fd9\u4e9b\u65b9\u6cd5\u53ef\u4ee5\u6839\u636e\u6570\u636e\u7684\u7279\u70b9\u81ea\u52a8\u6216\u624b\u52a8\u8c03\u6574\u53c2\u6570\uff0c\u4ece\u800c\u4f7f\u5f97\u6821\u6b63\u8fc7\u7a0b\u66f4\u52a0\u7cbe\u51c6\u548c\u51c6\u786e\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53;text-indent: 0pt;letter-spacing: 0.034em;\">\u901a\u8fc7\u5e94\u7528”scran”\u5305\u7684\u6279\u6b21\u6548\u5e94\u6821\u6b63\u529f\u80fd\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u6d88\u9664\u6570\u636e\u4e2d\u7684\u6279\u6b21\u504f\u5dee\uff0c\u4ece\u800c\u5f97\u5230\u66f4\u51c6\u786e\u3001\u66f4\u53ef\u9760\u7684\u5206\u6790\u7ed3\u679c\u3002\u8fd9\u4e3a\u4e0d\u540c\u5b9e\u9a8c\u6761\u4ef6\u4e0b\u7ec6\u80de\u7684\u6bd4\u8f83\u548c\u7ec6\u80de\u4e9a\u578b\u7684\u53d1\u73b0\u63d0\u4f9b\u4e86\u66f4\u6709\u529b\u7684\u652f\u6301\u3002\u540c\u65f6\uff0c\u8fd9\u4e5f\u4e3a\u8de8\u5b9e\u9a8c\u5ba4\u548c\u8de8\u5e73\u53f0\u7684\u6570\u636e\u6574\u5408\u63d0\u4f9b\u4e86\u4fbf\u5229\uff0c\u4f7f\u5f97\u6570\u636e\u7684\u53ef\u6bd4\u6027\u5f97\u4ee5\u7ef4\u6301\u3002<\/span><\/p>\n<p style=\"mso-style-parent: '';mso-margin-top-alt: auto;mso-margin-bottom-alt: auto;text-align: left;margin-left: 0.0pt;text-indent: 0.0pt;line-height: normal;mso-pagination: widow-orphan;font-size: 12.0pt;font-family: Times New Roman;mso-fareast-font-family: 'SimSun';mso-bidi-font-family: Times New Roman;font-weight: normal;mso-bidi-font-weight: normal;\"><span style=\"font-size: 15px;\"><span style=\"text-indent: 0pt;letter-spacing: 0.034em;font-family: \u5b8b\u4f53;font-variant-numeric: normal;font-variant-east-asian: normal;\">\u4ee5\u4e0a\u5c31\u662f\u5bf9R\u8bed\u8a00\u5305scran\u7684\u7b80\u5355\u4ecb\u7ecd\u5566\uff0c”scran”\u5305\u5728\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u53d1\u6325\u7740\u91cd\u8981\u4f5c\u7528\uff0c\u7279\u522b\u662f\u5728\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\u7684\u5206\u6790\u9886\u57df\u3002\u5b83\u4e3a\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4e86\u5f3a\u5927\u7684\u5de5\u5177\uff0c\u7528\u4e8e\u6570\u636e\u9884\u5904\u7406\u3001\u964d\u7ef4\u3001\u805a\u7c7b\u548c\u6279\u6b21\u6548\u5e94\u6821\u6b63\uff0c\u5e2e\u52a9\u4ed6\u4eec\u66f4\u597d\u5730\u7406\u89e3\u7ec6\u80de\u7684\u591a\u6837\u6027\u548c\u529f\u80fd\u3002\u901a\u8fc7<\/span><span style=\"text-indent: 0pt;letter-spacing: 0.034em;font-family: Calibri;font-variant-numeric: normal;font-variant-east-asian: normal;\">“scran”<\/span><span style=\"text-indent: 0pt;letter-spacing: 0.034em;font-family: \u5b8b\u4f53;font-variant-numeric: normal;font-variant-east-asian: normal;\">\u5305\uff0c\u7814\u7a76\u4eba\u5458\u80fd\u591f\u4ece\u6d77\u91cf\u7684\u5355\u7ec6\u80de\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u4e2d\u63d0\u53d6\u6709\u4ef7\u503c\u7684\u4fe1\u606f\uff0c\u63a8\u52a8\u751f\u7269\u5b66\u9886\u57df\u7684\u6df1\u5165\u7814\u7a76\u3002<\/span><span style=\"text-indent: 0pt;letter-spacing: 0.034em;font-family: Calibri;font-variant-numeric: normal;font-variant-east-asian: normal;\"><o:p> <\/o:p><\/span><span style=\"text-indent: 0pt;letter-spacing: 0.034em;\">    <\/span><\/span><\/p>\n<p style=\"text-align: left;margin-left: 0pt;text-indent: 0pt;line-height: normal;font-size: 12pt;font-family: "Times New Roman";font-weight: normal;\"><span style=\"text-indent: 0pt;color: rgb(0, 0, 0);font-family: helvetica;letter-spacing: 0.034em;font-variant-numeric: normal;font-variant-east-asian: normal;font-size: 15px;\">\u5c0f\u4f19\u4f34\u4eec\uff0c\u4eca\u5929\u6709\u6ca1\u6709\u5b66\u5230\u65b0\u77e5\u8bc6\u5462\uff0c\u60f3\u8981\u7ee7\u7eed\u4e86\u89e3R\u8bed\u8a00\u5185\u5bb9\u53ef\u4ee5\u6301\u7eed\u5173\u6ce8\u5c0f\u679c\u54e6\uff5e<\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);text-align: left;\">\n<section style=\"outline: 0px;font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;outline: 0px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;outline: 0px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;outline: 0px;\">\n<section style=\"outline: 0px;display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;outline: 0px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;outline: 0px;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"margin-top: -1em;padding: 3px 10px;outline: 0px;display: inline-block;vertical-align: top;color: rgb(0, 154, 68);\">\n<p style=\"outline: 0px;\"><strong style=\"outline: 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