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rgba(249, 172, 172, 0.19);min-width: 5%;height: auto;line-height: 1;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-right: 8px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 8px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);box-shadow: rgb(255, 255, 255) 8px 8px 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;background-color: rgb(249, 172, 172);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;background-color: rgb(249, 172, 172);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 8px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);box-shadow: rgb(255, 255, 255) 8px 8px 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 100 100 0%;height: auto;background-color: rgba(249, 172, 172, 0.19);\">\n<section style=\"transform: translate3d(7px, 0px, 0px);margin-top: 3px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;\">\n<p style=\"text-align: left;white-space: normal;\"><strong>\u4e0b\u8f7d\u5b89\u88c5<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">\u8f6f\u4ef6\u5b98\u7f51\uff1aThe Subread package (sourceforge.net)<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u53ef\u4ee5\u76f4\u63a5\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\u8fdb\u884c\u4e0b\u8f7d<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">wget https:\/\/jaist.dl.sourceforge.net\/project\/subread\/subread-<span class=\"code-snippet__number\">1.6<\/span>.0\/subread-<span class=\"code-snippet__number\">1.6<\/span>.0-Linux-x86_64.tar.gz<\/span><\/code><code><span class=\"code-snippet_outer\">tar -zxvf subread-<span class=\"code-snippet__number\">1.6<\/span>.0-Linux-x86_64.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;background-color: rgba(249, 172, 172, 0.19);min-width: 5%;height: auto;line-height: 1;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-right: 8px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 8px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);box-shadow: rgb(255, 255, 255) 8px 8px 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;background-color: rgb(249, 172, 172);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;background-color: rgb(249, 172, 172);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 8px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);box-shadow: rgb(255, 255, 255) 8px 8px 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 100 100 0%;height: auto;background-color: rgba(249, 172, 172, 0.19);\">\n<section style=\"transform: translate3d(7px, 0px, 0px);margin-top: 3px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;\">\n<p style=\"text-align: left;white-space: normal;\"><strong>\u8f93\u5165\u8f93\u51fa\u6570\u636e<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0em;white-space: normal;\" powered-by=\"xiumi.us\">\u8981\u4f7f\u7528featureCounts\u8f6f\u4ef6\uff0c\u6211\u4eec\u9700\u8981\u63d0\u4f9b\u4ee5\u4e0b\u8f93\u5165\u6570\u636e:<\/p>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">a\uff1a\u8bfb\u6bb5\u6620\u5c04\u6587\u4ef6\uff08Alignment file\uff09\uff1a\u8fd9\u662f\u7531\u6d4b\u5e8f\u6570\u636e\u6bd4\u5bf9\u8f6f\u4ef6\u751f\u6210\u7684\u6587\u4ef6\uff0c\u901a\u5e38\u662fBAM\u683c\u5f0f\u6216SAM\u683c\u5f0f\u7684\u6587\u4ef6\u3002\u8be5\u6587\u4ef6\u5305\u542b\u4e86\u6d4b\u5e8f\u6570\u636e\u4e2d\u6bcf\u4e2aread\u7684\u6620\u5c04\u4f4d\u7f6e\u4fe1\u606f\u3002<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">b\uff1a\u6ce8\u91ca\u6587\u4ef6\uff08Annotation 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onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEV8wyfQdyzQia3UCaaoDh9VFyqRIIr6bw3B34RHicK5jB2PyCLzcym4ibujBNOEZ2eo5nibld7ZApBvZQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"374\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;color: rgb(160, 160, 160);\" powered-by=\"xiumi.us\">\n<p style=\"text-align: center;white-space: normal;\">\u56fe1 SAF\u6587\u4ef6\u683c\u5f0f\uff08\u5217\u4ee5\u5236\u8868\u7b26\u5206\u9694\uff09<\/p>\n<\/section>\n<p style=\"text-indent: 0em;white-space: normal;\" powered-by=\"xiumi.us\">\u9664\u4e86\u8fd9\u4e9b\u5fc5\u9700\u7684\u8f93\u5165\u6570\u636e\u4e4b\u5916\uff0c\u60a8\u8fd8\u53ef\u4ee5\u63d0\u4f9b\u4e00\u4e9b\u53ef\u9009\u7684\u53c2\u6570\uff0c\u4ee5\u6839\u636e\u60a8\u7684\u5206\u6790\u9700\u6c42\u8fdb\u884c\u81ea\u5b9a\u4e49\u3002\u8fd9\u4e9b\u53c2\u6570\u53ef\u4ee5\u63a7\u5236\u8ba1\u6570\u7684\u7279\u5b9a\u8bbe\u7f6e\uff0c\u5982\u662f\u5426\u8003\u8651\u591a\u4e2a\u6620\u5c04\u4f4d\u7f6e\u3001\u662f\u5426\u8003\u8651\u94fe\u7279\u5f02\u6027\u7b49\u3002<\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;background-color: rgba(249, 172, 172, 0.19);min-width: 5%;height: auto;line-height: 1;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-right: 8px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 8px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);box-shadow: rgb(255, 255, 255) 8px 8px 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;background-color: rgb(249, 172, 172);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;background-color: rgb(249, 172, 172);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 8px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);box-shadow: rgb(255, 255, 255) 8px 8px 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 100 100 0%;height: auto;background-color: rgba(249, 172, 172, 0.19);\">\n<section style=\"transform: translate3d(7px, 0px, 0px);margin-top: 3px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;\">\n<p style=\"text-align: left;white-space: normal;\"><strong>\u53c2\u6570\u4ecb\u7ecd<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">featureCounts\u8f6f\u4ef6\u63d0\u4f9b\u4e86\u591a\u4e2a\u53c2\u6570\uff0c\u7528\u4e8e\u63a7\u5236\u8ba1\u6570\u8fc7\u7a0b\u548c\u5b9a\u91cf\u5206\u6790\u7684\u5404\u4e2a\u65b9\u9762\u3002\u4ee5\u4e0b\u662ffeatureCounts\u7684\u4e00\u4e9b\u5e38\u7528\u53c2\u6570\u7684\u7b80\u8981\u4ecb\u7ecd\uff1a<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">-a<annotation_file>\uff1a\u6307\u5b9a\u6ce8\u91ca\u6587\u4ef6\u7684\u8def\u5f84\u548c\u540d\u79f0\uff0c\u8be5\u6587\u4ef6\u63cf\u8ff0\u4e86\u57fa\u56e0\u6216\u8f6c\u5f55\u672c\u7684\u4f4d\u7f6e\u548c\u7279\u5f81\u3002<\/annotation_file><\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">-o<output_file>\uff1a\u6307\u5b9a\u8f93\u51fa\u6587\u4ef6\u7684\u8def\u5f84\u548c\u540d\u79f0\uff0c\u7528\u4e8e\u4fdd\u5b58\u8ba1\u6570\u7ed3\u679c\u3002<\/output_file><\/p>\n<p style=\"text-indent: 0em;white-space: 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href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-06-09 19:00:09<\/em><\/div>\n<p><content><\/p>\n<div class=\"reprint\">\n<p>\u8f6c\u81ea\u516c\u4f17\u53f7\uff1aR\u8bed\u8a00\u5b66\u5f92<br \/><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg5MDk3Mzg4OA==&mid=2247483899&idx=1&sn=414f5030e1aa8778c35e4861ed24967d\" target=\"_blank\" title=\"\u9605\u8bfb\u539f\u6587\" rel=\"noopener\">http:\/\/mp.weixin.qq.com\/s?__biz=Mzg5MDk3Mzg4OA==&mid=2247483899&idx=1&sn=414f5030e1aa8778c35e4861ed24967d<\/a><\/p>\n<\/div>\n<section style=\"font-size: 16px;margin-bottom: 24px;\">\n<section style=\"margin-top: 20px;margin-right: 0%;margin-left: 0%;text-align: left;justify-content: flex-start;transform: translate3d(-13px, 0px, 0px);display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: 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0em;white-space: normal;\" powered-by=\"xiumi.us\">\u8f6c\u5f55\u7ec4\u4e0a\u6e38\u5206\u6790\u4e2d\uff0c\u6211\u4eec\u9700\u8981\u5bf9reads\u8fdb\u884c\u7cbe\u786e\u7684\u8ba1\u6570\uff0c\u901a\u8fc7\u83b7\u5f97\u539f\u59cb\u8ba1\u6570\u6570\u636e\uff0c\u6211\u4eec\u53ef\u4ee5\u8fdb\u884c\u540e\u7eed\u7684\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u3001\u57fa\u56e0\u8868\u8fbe\u91cf\u6bd4\u8f83\u7b49\u5b9a\u91cf\u5206\u6790\u3002\u4eca\u5929\u5c0f\u5e08\u59b9\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u7684featureCounts\u5de5\u5177\u5c31\u53ef\u4ee5\u8f7b\u677e\u5b9e\u73b0\u8fd9\u4e2a\u529f\u80fd\u3002<\/p>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;background-color: rgba(249, 172, 172, 0.19);min-width: 5%;height: auto;line-height: 1;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-right: 8px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 8px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);box-shadow: rgb(255, 255, 255) 8px 8px 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;background-color: rgb(249, 172, 172);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;background-color: rgb(249, 172, 172);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 8px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);box-shadow: rgb(255, 255, 255) 8px 8px 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 100 100 0%;height: auto;background-color: rgba(249, 172, 172, 0.19);\">\n<section style=\"transform: translate3d(7px, 0px, 0px);margin-top: 3px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;\">\n<p style=\"text-align: left;white-space: normal;\"><strong>\u8fd9\u4e2a\u5de5\u5177\u662f\u4ec0\u4e48\uff1f<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">featureCounts\u662f\u4e00\u79cd\u5e38\u7528\u7684\u57fa\u56e0\u8ba1\u6570\u5de5\u5177\uff0c\u7528\u4e8e\u4ece\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6570\u636e\u4e2d\u83b7\u5f97\u57fa\u56e0\u6216\u8f6c\u5f55\u672c\u7684\u8ba1\u6570\u4fe1\u606f\u3002\u5b83\u53ef\u4ee5\u5904\u7406RNA-seq\u6216ChIP-seq\u7b49\u6d4b\u5e8f\u6570\u636e\uff0c\u5e76\u5c06reads\u6839\u636e\u5176\u5728\u57fa\u56e0\u6216\u8f6c\u5f55\u672c\u4e0a\u7684\u6620\u5c04\u4f4d\u7f6e\u8fdb\u884c\u8ba1\u6570\u3002\u8be5\u8f6f\u4ef6\u7684\u4e3b\u8981\u529f\u80fd\u662f\u5c06\u6bcf\u4e2a\u57fa\u56e0\u6216\u8f6c\u5f55\u672c\u4e0ereads\u5173\u8054\uff0c\u5e76\u751f\u6210\u8ba1\u6570\u77e9\u9635\uff0c\u5176\u4e2d\u6bcf\u884c\u8868\u793a\u4e00\u4e2a\u57fa\u56e0\u6216\u8f6c\u5f55\u672c\uff0c\u6bcf\u5217\u8868\u793a\u4e00\u4e2a\u6837\u672c\uff0c\u5355\u5143\u683c\u4e2d\u7684\u503c\u8868\u793a\u8be5\u57fa\u56e0\u6216\u8f6c\u5f55\u672c\u5728\u76f8\u5e94\u6837\u672c\u4e2d\u7684reads\u6570\u3002<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">featuresCounts\u901a\u5e38\u4e0e\u6d4b\u5e8f\u6570\u636e\u6bd4\u5bf9\u8f6f\u4ef6\uff08\u4f8b\u5982STAR\u3001HISAT2\u7b49\uff09\u7ed3\u5408\u4f7f\u7528\uff0c\u8fd9\u4e9b\u8f6f\u4ef6\u7528\u4e8e\u5c06reads\u6620\u5c04\u5230\u57fa\u56e0\u7ec4\u6216\u8f6c\u5f55\u7ec4\u4e0a\u3002\u4e00\u65e6reads\u88ab\u6bd4\u5bf9\u4e0a\uff0cfeaturesCounts\u4f1a\u6839\u636e\u6bd4\u5bf9\u7ed3\u679c\u8fdb\u884c\u8ba1\u6570\uff0c\u4ece\u800c\u5f97\u5230\u6bcf\u4e2a\u57fa\u56e0\u6216\u8f6c\u5f55\u672c\u7684reads\u6570\u3002<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u63a5\u4e0b\u6765\u5c31\u8ddf\u7740\u5c0f\u5e08\u59b9\u4e00\u8d77\u5b9e\u8df5\u8d77\u6765\u5427\uff01<\/p>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;background-color: rgba(249, 172, 172, 0.19);min-width: 5%;height: auto;line-height: 1;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-right: 8px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 8px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);box-shadow: rgb(255, 255, 255) 8px 8px 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;background-color: rgb(249, 172, 172);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;background-color: rgb(249, 172, 172);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 8px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);box-shadow: rgb(255, 255, 255) 8px 8px 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 100 100 0%;height: auto;background-color: rgba(249, 172, 172, 0.19);\">\n<section style=\"transform: translate3d(7px, 0px, 0px);margin-top: 3px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;\">\n<p style=\"text-align: left;white-space: normal;\"><strong>\u4e0b\u8f7d\u5b89\u88c5<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;white-space: normal;\">\u8f6f\u4ef6\u5b98\u7f51\uff1aThe Subread package (sourceforge.net)<\/p>\n<p style=\"text-indent: 0em;white-space: normal;\">\u53ef\u4ee5\u76f4\u63a5\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\u8fdb\u884c\u4e0b\u8f7d<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">wget https:\/\/jaist.dl.sourceforge.net\/project\/subread\/subread-<span class=\"code-snippet__number\">1.6<\/span>.0\/subread-<span class=\"code-snippet__number\">1.6<\/span>.0-Linux-x86_64.tar.gz<\/span><\/code><code><span class=\"code-snippet_outer\">tar -zxvf subread-<span class=\"code-snippet__number\">1.6<\/span>.0-Linux-x86_64.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;background-color: rgba(249, 172, 172, 0.19);min-width: 5%;height: auto;line-height: 1;\">\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;margin-right: 8px;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 8px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);box-shadow: rgb(255, 255, 255) 8px 8px 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;background-color: rgb(249, 172, 172);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;min-width: 5%;flex: 0 0 auto;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 16px;vertical-align: top;overflow: hidden;background-color: rgb(249, 172, 172);\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 8px;height: 8px;vertical-align: top;overflow: hidden;background-color: rgb(255, 255, 255);box-shadow: rgb(255, 255, 255) 8px 8px 0px;\">\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 100 100 0%;height: auto;background-color: rgba(249, 172, 172, 0.19);\">\n<section style=\"transform: translate3d(7px, 0px, 0px);margin-top: 3px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: justify;\">\n<p style=\"text-align: left;white-space: normal;\"><strong>\u8f93\u5165\u8f93\u51fa\u6570\u636e<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0em;white-space: normal;\" powered-by=\"xiumi.us\">\u8981\u4f7f\u7528featureCounts\u8f6f\u4ef6\uff0c\u6211\u4eec\u9700\u8981\u63d0\u4f9b\u4ee5\u4e0b\u8f93\u5165\u6570\u636e:<\/p>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">a\uff1a\u8bfb\u6bb5\u6620\u5c04\u6587\u4ef6\uff08Alignment file\uff09\uff1a\u8fd9\u662f\u7531\u6d4b\u5e8f\u6570\u636e\u6bd4\u5bf9\u8f6f\u4ef6\u751f\u6210\u7684\u6587\u4ef6\uff0c\u901a\u5e38\u662fBAM\u683c\u5f0f\u6216SAM\u683c\u5f0f\u7684\u6587\u4ef6\u3002\u8be5\u6587\u4ef6\u5305\u542b\u4e86\u6d4b\u5e8f\u6570\u636e\u4e2d\u6bcf\u4e2aread\u7684\u6620\u5c04\u4f4d\u7f6e\u4fe1\u606f\u3002<\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\">b\uff1a\u6ce8\u91ca\u6587\u4ef6\uff08Annotation 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