{"id":58954,"date":"2024-06-17T07:17:47","date_gmt":"2024-06-16T23:17:47","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=58954"},"modified":"2024-06-17T07:17:47","modified_gmt":"2024-06-16T23:17:47","slug":"estimate%e5%8c%85%e8%ae%a1%e7%ae%97%e5%a4%9a%e7%a7%8d%e5%be%97%e5%88%86%e5%b9%b6%e7%94%a8ggplot%e5%ae%9e%e7%8e%b0%e5%8f%af%e8%a7%86%e5%8c%96%ef%bc%9a%e4%bb%8e%e5%85%8d%e7%96%ab%e5%9f%ba%e8%b4%a8-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=58954","title":{"rendered":"Estimate\u5305\u8ba1\u7b97\u591a\u79cd\u5f97\u5206\u5e76\u7528ggplot\u5b9e\u73b0\u53ef\u89c6\u5316\uff1a\u4ece\u514d\u75ab\u57fa\u8d28\u5f97\u5206\u5230\u5f69\u8679\u4e4b\u6865\uff01"},"content":{"rendered":"<p><html><br \/>\n<head><br \/>\n<title><\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" 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15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;width: 30%;vertical-align: top;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 30% !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;text-align: center;line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" 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onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNXhQ4GD7nqK0jxIA9vNfaZMfcibQiaOCcR0jFpvjkSAu3UmKL82ohCV5Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"500\" style=\"vertical-align: middle;width: 100%;\"  data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section style=\"text-align: center;white-space: normal;margin-bottom: 8px;\"><span style=\"font-size: 20px;\"><strong><em><span style=\"color: rgb(62, 62, 62);\">\u4ec0\u4e48\u662festimate\u5305\uff1f<\/span><\/em><\/strong><\/span><\/section>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5728\u8fd9\u4e4b\u524d\uff0c\u5c0f\u679c\u5148\u7ed9\u5927\u5bb6\u5bf9estimate\u5305\u505a\u4e00\u4e2a\u7b80\u5355\u7684\u4ecb\u7ecd\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">estimate\u662f\u4e00\u4e2a\u5728\u80bf\u7624\u7ec4\u5b66\u7814\u7a76\u4e2d\u5e38\u7528\u7684R\u5305\uff0c\u5176\u4e3b\u8981\u529f\u80fd\u662f\u7528\u4e8e\u8bc4\u4f30\u80bf\u7624\u7ec4\u7ec7\u4e2d\u514d\u75ab\u7ec6\u80de\u6d78\u6da6\u7684\u7a0b\u5ea6\uff0c\u5e76\u5bf9\u514d\u75ab\u76f8\u5173\u7684\u4fe1\u53f7\u901a\u8def\u8fdb\u884c\u5206\u6790\u3002estimate\u57fa\u4e8e\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u8fdb\u884c\u8ba1\u7b97\uff0c\u4e3b\u8981\u5229\u7528\u4e86\u514d\u75ab\u548c\u80bf\u7624\u7ec6\u80de\u7279\u5f02\u7684\u57fa\u56e0\u8868\u8fbe\u6807\u8bb0\uff0c\u5e76\u6839\u636e\u8fd9\u4e9b\u6807\u8bb0\u7684\u8868\u8fbe\u6c34\u5e73\u4f30\u7b97\u7ec4\u7ec7\u4e2d\u514d\u75ab\u548c\u975e\u514d\u75ab\u7ec6\u80de\u7684\u6570\u91cf\u3002\u6700\u7ec8\uff0cestimate\u4f1a\u8f93\u51fa\u4e09\u4e2a\u6307\u6807\uff1aImmuneScore\u3001StromalScore\u548cESTIMATEScore\uff0c\u7528\u4e8e\u8bc4\u4f30\u514d\u75ab\u548c\u6210\u7ea4\u7ef4\u7ec6\u80de\u6d78\u6da6\u7684\u7a0b\u5ea6\u53ca\u5176\u5f71\u54cd\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5c0f\u679c\u63d0\u9192\uff0c\u4eca\u5929\u6211\u4eec\u8981\u5b66\u7684\u5c31\u662f\u901a\u8fc7estimate\u8ba1\u7b97ImmuneScore\u503c\u5e76\u5c06\u5176\u53ef\u89c6\u5316\uff0c\u540c\u65f6\u5728\u5b66\u4f1a\u8ba1\u7b97ImmuneScore\uff08\u514d\u75ab\u5f97\u5206\uff09\u7684\u57fa\u7840\u4e0a\u5c0f\u679c\u8fd8\u4f1a\u5411\u5927\u5bb6\u4ecb\u7ecd\u5982\u4f55\u8ba1\u7b97StromalScore\uff08\u57fa\u8d28\u5f97\u5206\uff09\uff0c\u90a3\u4e48\u5927\u5bb6\u5c31\u548c\u5c0f\u679c\u4e00\u8d77\u6765\u770b\u4e00\u4e0b\u5427\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<\/section>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.5\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_s2ZHCtbN9nDKgpfniawYJJzqxT6v6g-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNq0nuSrPNnVriasrjcop8ONDW3ss2ZHCtbN9nDKgpfniawYJJzqxT6v6g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"500\" style=\"vertical-align: middle;width: 100%;\"  data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-align: center;white-space: normal;margin-bottom: 16px;\"><strong><em><span style=\"font-size: 20px;color: rgb(62, 62, 62);\">\u6570\u636e\u5bfc\u5165\u53ca\u9884\u5904\u7406<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u9996\u5148\u6211\u4eec\u9700\u8981\u5b89\u88c5\u5e76\u8f7d\u5165\u4e00\u4e2a\u53eb\u505a\u201cestimate\u201d\u7684\u5305\uff0c\u8fd9\u4e2a\u5305\u53ef\u4ee5\u7528\u6765\u8ba1\u7b97Immune Score\u7684\u5206\u503c\u54e6\u3002<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">library(estimate)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\u6211\u4eec\u6765\u5bfc\u5165\u6211\u4eec\u51c6\u5907\u597d\u7684\u6570\u636e\uff1a<\/span><\/p>\n<section powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">rawdata_file &lt;- <span class=\"code-snippet__string\">\"tumor_all_gene_exp.txt\"<\/span><\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\u6211\u4eec\u4f7f\u7528\u201cfilterCommonGenes\u201d\u51fd\u6570\u5bf9\u6570\u636e\u96c6\u8fdb\u884c\u8fc7\u6ee4\uff0c\u5e76\u5c06\u7ed3\u679c\u4fdd\u5b58\u5230\u201cmy.gct\u201d\u7684\u6587\u4ef6\u4e2d\u3002\u5728\u8fd9\u4e2a\u51fd\u6570\u4e2d\uff0c\u6211\u4eec\u8fd8\u8981\u6307\u5b9a\u201cGeneSymbol\u201d\u4f5c\u4e3a\u57fa\u56e0\u7684ID\uff1a<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">filterCommonGenes(input.f=rawdata_file, <\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;output.f=<span class=\"code-snippet__string\">\"my.gct\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;id=<span class=\"code-snippet__string\">\"GeneSymbol\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><br  \/><\/section>\n<\/section>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><br  \/><\/section>\n<\/section>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.49899799599198397\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_PSgMgn9cx3xIMRpoK7HUMUxLr0HL3A.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNszG7picpYriceh3kdysIicAjyUtPSgMgn9cx3xIMRpoK7HUMUxLr0HL3A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"499\" style=\"vertical-align: middle;width: 100%;\"  data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;font-size: 20px;\" powered-by=\"xiumi.us\">\n<p style=\"margin-bottom: 8px;\"><em><strong>\u8ba1\u7b97\u514d\u75ab\u5f97\u5206<\/strong><\/em><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u7136\u540e\u6211\u4eec\u9700\u8981\u4f7f\u7528\u201cestimateScore\u201d\u51fd\u6570\u6765\u8ba1\u7b97\u514d\u75ab\u5f97\u5206\u3002\u5728\u8fd9\u4e2a\u51fd\u6570\u4e2d\uff0c\u6211\u4eec\u9700\u8981\u6307\u5b9a\u8f93\u5165\u6587\u4ef6\u7684\u8def\u5f84\uff0c\u4ee5\u53ca\u8f93\u51fa\u6587\u4ef6\u7684\u8def\u5f84\u3002\u6211\u4eec\u8fd8\u8981\u6307\u5b9a\u6570\u636e\u7684\u5e73\u53f0\uff08\u4f8b\u5982affymetrix\uff09\uff1a<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">estimateScore(input.ds = <span class=\"code-snippet__string\">\"my.gct\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;output.ds=<span class=\"code-snippet__string\">\"estimate_score.gct\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;platform=<span class=\"code-snippet__string\">\"affymetrix\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><span style=\"font-size: 14px;\">\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u9700\u8981\u8bfb\u53d6\u8f93\u51fa\u6587\u4ef6\u201cestimate_score.gct\u201d\u4e2d\u7684\u6570\u636e\uff0c\u5e76\u5c06\u5176\u4fdd\u5b58\u5230\u540d\u4e3a\u201craw_data\u201d\u7684\u6570\u636e\u96c6\u4e2d\uff1a<\/span><br  \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">raw_data = read.table(<span class=\"code-snippet__string\">\"estimate_score.gct\"<\/span>,skip = <span class=\"code-snippet__number\">2<\/span>,header = F,quote = <span class=\"code-snippet__string\">'',sep = '<\/span>t',stringsAsFactors=FALSE)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(raw_data)=raw_data[,<span class=\"code-snippet__number\">1<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">raw_data= as.data.frame(t(raw_data[,<span class=\"code-snippet__number\">3<\/span>:ncol(raw_data)]))<\/span><\/code><code><span class=\"code-snippet_outer\">row.names(raw_data)&lt;-c(<span class=\"code-snippet__number\">1<\/span>:nrow(raw_data))<\/span><\/code><code><span class=\"code-snippet_outer\">colnames(raw_data)[<span class=\"code-snippet__number\">1<\/span>] &lt;- c(<span class=\"code-snippet__string\">\"sample\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i in <span class=\"code-snippet__number\">2<\/span>:<span class=\"code-snippet__number\">5<\/span>){<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp;raw_data[,i] &lt;- as.numeric(as.character(raw_data[,i]))<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u6211\u4eec\u4e00\u8d77\u6765\u770b\u4e0b\u8f93\u51fa\u7684\u6570\u636e\u5427\uff1a<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8754512635379061\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_Yy2y56EhxV5uaU01M9BIVCkA1fYJ5w.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtND9FzjxgjQnnQ8EVpm2mn3NJsYy2y56EhxV5uaU01M9BIVCkA1fYJ5w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u7136\u540e\uff0c\u6211\u4eec\u9700\u8981\u8bfb\u53d6\u51c6\u5907\u597d\u7684\u201cgroup.txt\u201d\u7684\u6587\u4ef6\uff0c\u8fd9\u4e2a\u6587\u4ef6\u5305\u542b\u4e86\u6bcf\u4e2a\u6837\u672c\u7684\u540d\u79f0\u4ee5\u53ca\u5176\u5bf9\u5e94\u7684\u96c6\u7fa4\uff0c\u6211\u4eec\u9700\u8981\u5c06\u5176\u4e0e\u4e0a\u9762\u7684\u7ed3\u679c\u6587\u4ef6\u5408\u5e76\u624d\u80fd\u65b9\u4fbf\u4e4b\u540e\u7684\u53ef\u89c6\u5316\u5de5\u4f5c\uff1a<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">phenotype_file &lt;- read.table(<span class=\"code-snippet__string\">'group.txt'<\/span>,header = T,sep = <span class=\"code-snippet__string\">'t'<\/span>,quote = <span class=\"code-snippet__string\">'',stringsAsFactors=FALSE)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">phenotype_file &lt;- phenotype_file[phenotype_file[,1] %in% raw_data[,\"sample\"],]<\/span><\/code><code><span class=\"code-snippet_outer\">merge_matrix &lt;- left_join(phenotype_file,raw_data,by=\"sample\") #\u5408\u5e76\u6570\u636e\u96c6<\/span><\/code><code><span class=\"code-snippet_outer\">my_comparisons &lt;- list( c(\"cluster1\", \"cluster2\"),c(\"cluster2\",\"cluster3\"),c(\"cluster1\", \"cluster3\"))<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br  \/><\/p>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4400871459694989\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_5b6n1ktWiauTdCD5ibDmDlYhn4Gvlg-2.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNylh9Ot54aFJuqnzRED27naywY85b6n1ktWiauTdCD5ibDmDlYhn4Gvlg\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"459\" style=\"vertical-align: middle;width: 100%;\"  data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;font-size: 20px;\" powered-by=\"xiumi.us\">\n<section style=\"margin-bottom: 8px;\"><em><strong>\u8ba1\u7b97\u7ed3\u679c\u53ef\u89c6\u5316<\/strong><\/em><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5728\u4e0a\u9762\u7684\u6b65\u9aa4\u4e2d\uff0c\u6211\u4eec\u6210\u529f\u7684\u8ba1\u7b97\u51fa\u4e86Immune Score\u7684\u503c\uff0c\u73b0\u5728\u6211\u4eec\u901a\u8fc7\u6211\u4eec\u719f\u6089\u7684ggplot2\u5305\u6765\u5b9e\u73b0\u8ba1\u7b97\u7ed3\u679c\u7684\u53ef\u89c6\u5316\uff1a<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u8bbe\u7f6emy_labely\u6807\u7b7e<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">max_labely &lt;- max(merge_matrix[,<span class=\"code-snippet__string\">\"ImmuneScore\"<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\">min_labely &lt;- min(merge_matrix[,<span class=\"code-snippet__string\">\"ImmuneScore\"<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\">my_labely &lt;- c(max_labely+<span class=\"code-snippet__number\">1350<\/span>, max_labely+<span class=\"code-snippet__number\">1000<\/span>, max_labely+<span class=\"code-snippet__number\">650<\/span>, max_labely+<span class=\"code-snippet__number\">300<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br  \/><\/p>\n<section style=\"line-height: 0;text-align: center;margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 20%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.42061855670103093\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_M4YPbLrMhk5rkKib7KWjdWUhv6ZXoA-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_jpg\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNHMkkJngnf5Q03jSVl55kdgmnXM4YPbLrMhk5rkKib7KWjdWUhv6ZXoA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"485\" style=\"vertical-align: middle;width: 100%;\"  data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"text-align: center;font-size: 20px;\" powered-by=\"xiumi.us\">\n<section style=\"margin-bottom: 8px;\"><em><strong>\u7ed8\u5236\u7bb1\u7ebf\u56fe<\/strong><\/em><\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">ggboxplot(merge_matrix, x = <span class=\"code-snippet__string\">\"Group\"<\/span>, y = <span class=\"code-snippet__string\">\"ImmuneScore\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp;color = <span class=\"code-snippet__string\">\"black\"<\/span>,alpha = <span class=\"code-snippet__number\">0.8<\/span>,size=<span class=\"code-snippet__number\">.3<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; add = <span class=\"code-snippet__string\">\"jitter\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp;add.params = <span class=\"code-snippet__built_in\">list<\/span>(color = <span class=\"code-snippet__string\">\"Group\"<\/span>,alpha = <span class=\"code-snippet__number\">.5<\/span>,size = <span class=\"code-snippet__number\">0.8<\/span>)) +stat_compare_means(comparisons = my_comparisons,method = <span class=\"code-snippet__string\">\"t.test\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; label.y = my_labely,na.rm=TRUE)+<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp;stat_summary(fun.y = <span class=\"code-snippet__string\">\"mean\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;geom = <span class=\"code-snippet__string\">\"point\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;color = <span class=\"code-snippet__string\">\"#485c70\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;shape = <span class=\"code-snippet__number\">8<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;size = <span class=\"code-snippet__number\">3<\/span>) +<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br  \/><\/p>\n<section style=\"font-size: 18px;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><em><strong>\u6dfb\u52a0\u6807\u9898\u548c\u8c03\u6574\u7ed8\u56fe\u7ec6\u8282<\/strong><\/em><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">ggtitle(<span class=\"code-snippet__string\">\"Immune Score\"<\/span>) +theme_bw()+<\/span><\/code><code><span class=\"code-snippet_outer\">theme(legend.position=<span class=\"code-snippet__string\">\"none\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; plot.title = element_text(size=<span class=\"code-snippet__number\">15<\/span>,face=<span class=\"code-snippet__string\">\"bold\"<\/span>,hjust = <span class=\"code-snippet__number\">0.5<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; axis.text.x=element_text(colour=<span class=\"code-snippet__string\">\"black\"<\/span>), <\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; axis.text.y=element_text(colour=<span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\">panel.grid.major = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;panel.grid.minor = element_blank()) +<\/span><\/code><\/pre>\n<\/section>\n<p style=\"white-space: normal;\" powered-by=\"xiumi.us\"><br  \/><\/p>\n<section style=\"text-align: left;font-size: 18px;color: rgb(62, 62, 62);\" powered-by=\"xiumi.us\">\n<p><strong><em>\u8bbe\u7f6ex\u8f74\u548cy\u8f74\u540d\u79f0\u4ee5\u53cay\u8f74\u663e\u793a\u8303\u56f4<\/em><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">ylab(<span class=\"code-snippet__string\">\"Immune Score\"<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">xlab(<span class=\"code-snippet__string\">\"Group\"<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">ylim(min_labely<span class=\"code-snippet__number\">-500<\/span>, max_labely+<span class=\"code-snippet__number\">1500<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">box_plot_pdf &lt;- <span class=\"code-snippet__string\">\"path\/to\/immune_score_box.pdf\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">ggsave(box_plot_pdf,width=<span class=\"code-snippet__number\">17.8<\/span>,height=<span class=\"code-snippet__number\">17.8<\/span>,units = <span class=\"code-snippet__string\">'cm'<\/span>, dpi = <span class=\"code-snippet__number\">500<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u6700\u540e\uff0c\u6211\u4eec\u4f7f\u7528 ggsave \u51fd\u6570\u5c06\u7ed8\u5236\u597d\u7684\u56fe\u4fdd\u5b58\u4e3a PDF \u683c\u5f0f\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u73b0\u5728\u548c\u6548\u679c\u4e00\u8d77\u6765\u770b\u4e0b\u6700\u540e\u7ed8\u5236\u597d\u7684\u56fe\u957f\u4ec0\u4e48\u6837\u5427\uff01<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4169278996865204\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_ynZAUA2v39CSxEdzcOSoxaicMstiaA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtN4iad0t5ZHibCM3TXHR5uq9TQkibAcynZAUA2v39CSxEdzcOSoxaicMstiaA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"638\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u600e\u4e48\u6837\uff0c\u4f60\u5b66\u4f1a\u600e\u4e48\u4f7f\u7528estimate\u5305\u4e86\u561b? \u66f4\u591a\u5b66\u4e60\u8d44\u6e90\u8bf7\u5927\u5bb6\u79fb\u6b65\u5c0f\u679c\u4e13\u5c5e\u4e91\u751f\u4fe1\u5e73\u53f0\u641c\u7d22\u66f4\u591a\u8d44\u6e90\u54e6\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">StromalScore<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u6709\u4e86\u8ba1\u7b97ImmuneScore\u7684\u57fa\u7840\uff0c\u8ba1\u7b97StromalScore\u4e5f\u662f\u540c\u6837\u7684\u8ba1\u7b97\u65b9\u6cd5\u548c\u53ef\u89c6\u5316\u65b9\u6cd5\uff0c\u53ea\u8981\u63d0\u53d6\u7684\u662f\u7ed3\u679c\u6587\u4ef6\u4e2d\u7684StromalScore\u5217\u5373\u53ef\uff1a<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8744186046511628\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_icBWSfmIFicxpoKgfpCiaqPyY7Opjg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNyRpiaF30Zc8libn4XH4vSLKVxEXyficBWSfmIFicxpoKgfpCiaqPyY7Opjg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"430\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u90a3\u4e48\u6211\u4eec\u518d\u6765\u770b\u770b\u6700\u540eStromalScore\u7684\u53ef\u89c6\u5316\u7ed3\u679c\uff1a<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.41590214067278286\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_KuqH8s4ibjptN5B64oTtWqv4n0QNIQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtNOvckg72WOuoHhEBSQC06w2xKxKuqH8s4ibjptN5B64oTtWqv4n0QNIQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"654\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u600e\u4e48\u6837\uff0c\u4eca\u5929\u7684\u56fe\u4f60\u5b66\u4f1a\u600e\u4e48\u7ed8\u5236\u4e86\u5417\uff1f<\/span><em style=\"letter-spacing: 0.578px;color: rgb(0, 0, 0);font-size: 17px;line-height: 1.59em;\"><\/em><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section data-tools=\"135\u7f16\u8f91\u5668\" data-id=\"105054\" style=\"font-size: 16px;\">\n<section style=\"margin: 10px auto;text-align: center;\">\n<section style=\"padding-top: 30px;padding-bottom: 30px;background-color: rgb(242, 246, 255);border-radius: 7px;\">\n<section style=\"display: flex;justify-content: space-evenly;align-items: center;\">\n<section data-width=\"26%\" style=\"width: 26%;max-width: 26% !important;\">\n<section style=\"padding: 7px;border-radius: 15px;box-shadow: rgba(169, 187, 223, 0.45) 4px 4px 8px, rgb(255, 255, 255) -2.5px -2.5px 5px;\">\n<section data-width=\"100%\" style=\"width: 100%;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1.005281690140845\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_0WFLKBMicbm6iawtkrNrMxpSVU9GBQ-11.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/zcicibWZessAxec5nH6tybm0EXxCaibxbtN261llpmfF6btPBTUKkibEdnRz5Y0WFLKBMicbm6iawtkrNrMxpSVU9GBQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"568\" data-width=\"100%\" style=\"vertical-align: inherit;width: 100%;display: block;border-radius: 7px;\" title=\"\u5c0f\u4e91-\u5fae\u4fe1\u4e8c\u7ef4\u7801.png\"  data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<\/section>\n<section data-width=\"62%\" style=\"width: 62%;max-width: 62% !important;\">\n<section style=\"display: inline-block;\">\n<section style=\"padding: 7px 15px;border-radius: 20px;box-shadow: rgba(169, 187, 223, 0.45) 6px 6px 8px inset, rgb(255, 255, 255) -2.5px -2.5px 5px inset;\">\n<section data-brushtype=\"text\" style=\"font-size: 14px;letter-spacing: 1.5px;color: rgb(111, 133, 172);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u5fae\u4fe1\u53f7 | 18502195490<\/span><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-top: 12px;padding: 7px 15px;border-radius: 20px;box-shadow: rgba(169, 187, 223, 0.45) 6px 6px 8px inset, rgb(255, 255, 255) -2.5px -2.5px 5px inset;\">\n<section data-brushtype=\"text\" style=\"font-size: 14px;letter-spacing: 1.5px;color: rgb(111, 133, 172);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u77e5\u4e4e | \u751f\u4fe1\u679c<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"letter-spacing: 0.578px;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193&nbsp;<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"http:\/\/www.biocloudservice.com\/home.html\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Estimate\u5305\u8ba1\u7b97\u591a\u79cd\u5f97\u5206\u5e76\u7528ggplot\u5b9e\u73b0\u53ef\u89c6\u5316\uff1a\u4ece\u514d\u75ab\u57fa\u8d28\u5f97\u5206\u5230\u5f69\u8679\u4e4b\u6865\uff01 \u539f\u521b&nbsp;&#038;nbs [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58954"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=58954"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58954\/revisions"}],"predecessor-version":[{"id":58966,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/58954\/revisions\/58966"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=58954"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=58954"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=58954"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}