{"id":59077,"date":"2024-06-17T07:34:40","date_gmt":"2024-06-16T23:34:40","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=59077"},"modified":"2024-06-17T07:34:40","modified_gmt":"2024-06-16T23:34:40","slug":"%e5%bf%ab%e6%9d%a5%e5%ad%a6%ef%bc%81%e4%bd%a0%e4%b8%8d%e7%9f%a5%e9%81%93%e7%9a%84tss%e5%af%8c%e9%9b%86%e7%83%ad%e5%9b%be%e4%b8%ad%e7%9a%84%e5%b0%8f%e7%bb%86%e8%8a%82-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=59077","title":{"rendered":"\u5feb\u6765\u5b66\uff01\u4f60\u4e0d\u77e5\u9053\u7684TSS\u5bcc\u96c6\u70ed\u56fe\u4e2d\u7684\u5c0f\u7ec6\u8282"},"content":{"rendered":"<p><html><br \/>\n<head><br \/>\n<title><\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" 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span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag 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style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;line-height: 0;\">\n<section>\n<section style=\"display: inline-block;vertical-align: middle;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 0;\">\n<section style=\"width: 111px;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 1.75em;text-indent: 0em;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;height: auto;width: 111px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_Xkz3aJGClianeaJMFaPKsbDRmfsHNw-8.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPdqELib8iaLzsvaTzaicfA3URAEIyVaH5J7WwYnm1GpxBmYrwXkz3aJGClianeaJMFaPKsbDRmfsHNw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"400\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section style=\"display: contents;\" powered-by=\"xiumi.us\">\n<section style=\"display: contents;\">\n<section style=\"display: contents;\">\n<section style=\"height: 0px;overflow: hidden;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;caret-color: red;\">&nbsp;<\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 10px 0% 8px;justify-content: flex-start;display: flex;flex-flow: row nowrap;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-left: 3px solid rgb(219, 219, 219);border-bottom-left-radius: 0px;padding-left: 8px;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"color: rgba(0, 0, 0, 0.5);text-align: justify;font-size: 14px;\" powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\">\u4e00\u6587\u641e\u5b9aTSS\u5bcc\u96c6\u70ed\u56fe<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">&nbsp;<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4eca\u5929\u5c0f\u679c\u6709\u70b9\u5fe7\u4f24\uff0c\u4e3a\u4ec0\u4e48\u8bba\u6587\u91cc\u9762\u7684TSS\u5bcc\u96c6\u56fe\u90a3\u4e48\u7f8e\u89c2\uff0c\u5c0f\u679c\u7684\u5374\u7565\u663e\u4e11\u964b\u5462<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e0b\u9762\u662f\u8bba\u6587\u4e2d\u7684TSS\u5bcc\u96c6\u56fe\uff0c\u5927\u5bb6\u6b23\u8d4f\u4e00\u4e0b<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br  \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8194444444444444\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_qUve0SeQNG5pKticX0h4mIQB6BCWnA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPdqELib8iaLzsvaTzaicfA3Uj1J8zmwIZ9U0Wq7kjxr9evicVyqUve0SeQNG5pKticX0h4mIQB6BCWnA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"288\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e0b\u9762\u5462\u5219\u662f\u76f8\u6bd4\u8d77\u6765\u7565\u663e\u4e0d\u884c\u7684\u5c0f\u679c\u505a\u7684TSS\u5bcc\u96c6\u56fe\uff0c\u5927\u5bb6\u6b23\u8d4f\u4e00\u4e0b<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">&nbsp;<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"3.58955223880597\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_UTesQvN7iazrPmDFhl1GJpaFkoMc8Q.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPdqELib8iaLzsvaTzaicfA3UfvetFBGwZNZ7wmLA4wibMhlXianUTesQvN7iazrPmDFhl1GJpaFkoMc8Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"134\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e0d\u6bd4\u4e0d\u77e5\u9053\uff0c\u4e00\u6bd4\u5413\u4e00\u8df3\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5c0f\u679c\u505a\u7684\u56fe\u91cc\u9762\u6709\u5f88\u591a\u7684\u201d\u9ed1\u7ebf\u201d\uff0c\u5341\u5206\u5f71\u54cd\u7f8e\u89c2!<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u8fd9\u53ef\u600e\u4e48\u529e\u5462\uff0c\u5c0f\u679c\u8d76\u7d27\u53c8\u53bb\u4ed4\u7ec6\u9605\u8bfb\u4e86deeptools\u7684\u5e2e\u52a9\u6587\u6863<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">--plotType {lines,fill,se,<span class=\"code-snippet__built_in\">std<\/span>}<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<span class=\"code-snippet__string\">\"lines\"<\/span> will plot the profile line based on the<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;average type selected. <span class=\"code-snippet__string\">\"fill\"<\/span> fills the region between<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;zero <span class=\"code-snippet__keyword\">and<\/span> the profile curve. The fill in color is semi<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;transparent to distinguish different profiles. <span class=\"code-snippet__string\">\"se\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<span class=\"code-snippet__keyword\">and<\/span> <span class=\"code-snippet__string\">\"std\"<\/span> color the region between the profile <span class=\"code-snippet__keyword\">and<\/span> the<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;standard error <span class=\"code-snippet__keyword\">or<\/span> standard deviation of the data.<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;(<span class=\"code-snippet__keyword\">default<\/span>: lines)<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp;--sortUsingSamples SORTUSINGSAMPLES [SORTUSINGSAMPLES ...]<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<span class=\"code-snippet__function\">List of sample <span class=\"code-snippet__title\">numbers<\/span> <span class=\"code-snippet__params\">(order as in matrix)<\/span>, which are<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;used by --sortUsing <span class=\"code-snippet__keyword\">for<\/span> sorting. If no value is <span class=\"code-snippet__built_in\">set<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;it uses all samples. Example: --sortUsingSamples 1 3<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<span class=\"code-snippet__params\">(<span class=\"code-snippet__keyword\">default<\/span>: None)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp;--linesAtTickMarks &nbsp; &nbsp;Draw dashed lines from all tick marks through the<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;heatmap. This is then similar to the dashed line draw<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;at region bounds when <span class=\"code-snippet__keyword\">using<\/span> a reference point <span class=\"code-snippet__keyword\">and<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;--sortUsing <span class=\"code-snippet__title\">region_length<\/span> <span class=\"code-snippet__params\">(<span class=\"code-snippet__keyword\">default<\/span>: False)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp;--clusterUsingSamples CLUSTERUSINGSAMPLES [CLUSTERUSINGSAMPLES ...]<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;List of sample <span class=\"code-snippet__title\">numbers<\/span> <span class=\"code-snippet__params\">(order as in matrix)<\/span>, that are<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;used <span class=\"code-snippet__keyword\">for<\/span> clustering by --kmeans <span class=\"code-snippet__keyword\">or<\/span> --hclust <span class=\"code-snippet__keyword\">if<\/span> <span class=\"code-snippet__keyword\">not<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br  \/><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;given, all samples are taken into account <span class=\"code-snippet__keyword\">for<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;clustering. Example: --ClusterUsingSamples 1 3<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<span class=\"code-snippet__params\">(<span class=\"code-snippet__keyword\">default<\/span>: None)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet_outer\"> &nbsp;--averageTypeSummaryPlot <\/span>{mean,median,min,max,<span class=\"code-snippet__built_in\">std<\/span>,sum}<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;Define the type of statistic that should be plotted in<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;the summary image above the heatmap. The options are:<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<span class=\"code-snippet__string\">\"mean\"<\/span>, <span class=\"code-snippet__string\">\"median\"<\/span>, <span class=\"code-snippet__string\">\"min\"<\/span>, <span class=\"code-snippet__string\">\"max\"<\/span>, <span class=\"code-snippet__string\">\"sum\"<\/span> <span class=\"code-snippet__keyword\">and<\/span> <span class=\"code-snippet__string\">\"std\"<\/span>.<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;(<span class=\"code-snippet__keyword\">default<\/span>: mean)<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp;--missingDataColor MISSINGDATACOLOR<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;If --missingDataAsZero was <span class=\"code-snippet__keyword\">not<\/span> <span class=\"code-snippet__built_in\">set<\/span>, such cases will be<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;colored in black by <span class=\"code-snippet__keyword\">default<\/span>. Using <span class=\"code-snippet__keyword\">this<\/span> parameter, a<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;different color can be <span class=\"code-snippet__built_in\">set<\/span>. A value between <span class=\"code-snippet__number\">0<\/span> <span class=\"code-snippet__keyword\">and<\/span> <span class=\"code-snippet__number\">1<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<span class=\"code-snippet__function\">will be used <span class=\"code-snippet__keyword\">for<\/span> a gray <span class=\"code-snippet__title\">scale<\/span> <span class=\"code-snippet__params\">(black is <span class=\"code-snippet__number\">0<\/span>)<\/span>. For a <span class=\"code-snippet__built_in\">list<\/span><\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;of possible color names see: http:<span class=\"code-snippet__comment\">\/\/packages.python.or<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;g\/ete2\/reference\/reference_svgcolors.html. Other<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;colors can be specified <span class=\"code-snippet__keyword\">using<\/span> the <span class=\"code-snippet__meta\">#rrggbb notation.<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<span class=\"code-snippet__params\">(<span class=\"code-snippet__keyword\">default<\/span>: black)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp;--colorNumber COLORNUMBER<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;N.B., --colorList is required <span class=\"code-snippet__keyword\">for<\/span> an effect. This<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;controls the number of transitions from one color to<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;the other. If --colorNumber is the number of colors in<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;--colorList then there will be no transitions between<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;the colors. <span class=\"code-snippet__params\">(<span class=\"code-snippet__keyword\">default<\/span>: <span class=\"code-snippet__number\">256<\/span>)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp;--heatmapHeight HEATMAPHEIGHT<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;Plot height in cm. The <span class=\"code-snippet__keyword\">default<\/span> <span class=\"code-snippet__keyword\">for<\/span> the heatmap height<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;is 28. The minimum value is 3 <span class=\"code-snippet__keyword\">and<\/span> the maximum is 100.<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<span class=\"code-snippet__params\">(<span class=\"code-snippet__keyword\">default<\/span>: <span class=\"code-snippet__number\">28<\/span>)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp;--heatmapWidth HEATMAPWIDTH<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;Plot width in cm. The <span class=\"code-snippet__keyword\">default<\/span> value is 4 The minimum<\/span><\/code><code><span class=\"code-snippet_outer\"> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;value is 1 <span class=\"code-snippet__keyword\">and<\/span> the maximum is 100. <span class=\"code-snippet__params\">(<span class=\"code-snippet__keyword\">default<\/span>: <span class=\"code-snippet__number\">4<\/span>)<\/span><\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e0a\u9762\u51e0\u4e2a\u53c2\u6570\u662f\u5c0f\u679c\u4ed4\u7ec6\u9605\u8bfb\u4e4b\u540e\u53d1\u73b0\u6709\u53ef\u80fd\u5f71\u54cd\u201c\u9ed1\u7ebf\u201d\u7684\u5173\u952e\u53c2\u6570\uff0c\u4e0d\u8fc7\u5c0f\u679c\u611f\u89c9\u4e00\u4e2a\u4e00\u4e2a\u53bb\u5b9e\u9a8c\u592a\u9ebb\u70e6\u4e86\uff0c\u53ea\u80fd\u53bb\u6c42\u52a9\u4e07\u80fd\u7684\u5ea6\u5a18\uff0c\u91cc\u9762\u6709\u5c0f\u4f19\u4f34\u8bf4\u51fa\u73b0\u9ed1\u7ebf\u662f\u56e0\u4e3a\u5728\u4f7f\u7528computeMatrix\u5f62\u6210\u77e9\u9635\u6587\u4ef6\u6ca1\u6709\u6dfb\u52a0\u53c2\u6570&#8211;missingDataAsZero\uff01\u5c0f\u679c\u6ca1\u6709\u60f3\u5230\u662f\u56e0\u4e3a\u5f62\u6210\u77e9\u9635\u6587\u4ef6\u7684\u95ee\u9898\uff0c\u5e78\u4e8f\u5c0f\u679c\u6ca1\u6709\u4e00\u4e2a\u4e2a\u53c2\u6570\u5b9e\u9a8c\uff0c\u90a3\u6015\u4e0d\u662f\u767d\u767d\u6d6a\u8d39\u65f6\u95f4\uff01<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br  \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5728\u4e86\u89e3\u5230\u4e86\u95ee\u9898\u6240\u5728\u4e4b\u540e\uff0c\u5c0f\u679c\u5feb\u9a6c\u52a0\u97ad\u53bb\u5b9e\u9a8c\u4e86\u4e00\u4e0b\uff0c\u4e0b\u9762\u662f\u5c0f\u679c\u7684\u4f7f\u7528computeMatrix\u547d\u4ee4\u5f62\u6210\u77e9\u9635\u6587\u4ef6\u7684\u53c2\u6570\uff1a<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">computeMatrix &nbsp;reference-point &nbsp;--referencePoint &nbsp;TSS &nbsp;-p <span class=\"code-snippet__number\">15<\/span> -b <span class=\"code-snippet__number\">10000<\/span> -a <span class=\"code-snippet__number\">10000<\/span> -R gene.bed -S &nbsp;test.bw &nbsp;--skipZeros --missingDataAsZero &nbsp; -o test_TSS.gz &nbsp;--outFileSortedRegions test_genes.bed<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e0b\u9762\u662f\u53c2\u6570\u7684\u89e3\u91ca\uff1a<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">reference-point # \u9009\u62e9\u6a21\u5f0f<\/span><\/code><code><span class=\"code-snippet_outer\"> -p <span class=\"code-snippet__number\">15<\/span> # \u7ebf\u7a0b\u6570<\/span><\/code><code><span class=\"code-snippet_outer\"> --referencePoint TSS &nbsp;# \u9009\u62e9\u53c2\u8003\u70b9\uff0c\u8fd8\u53ef\u4ee5\u9009\u62e9TES, center<\/span><\/code><code><span class=\"code-snippet_outer\"> -b <span class=\"code-snippet__number\">10000<\/span> -a <span class=\"code-snippet__number\">10000<\/span> &nbsp;# \u611f\u5174\u8da3\u7684\u533a\u57df\uff0c-b\u4e0a\u6e38\uff0c-a\u4e0b\u6e38<\/span><\/code><code><span class=\"code-snippet_outer\"> -R &nbsp;# \u57fa\u56e0\u6ce8\u91ca\u4fe1\u606f<\/span><\/code><code><span class=\"code-snippet_outer\"> -S &nbsp;# \u63d0\u4f9b\u7684 bigwig \u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\"> --skipZeros &nbsp;# \u662f\u5426\u5305\u542b\u96f6\u5206\u533a\u57df<\/span><\/code><code><span class=\"code-snippet_outer\">--missingDataAsZero # \u4e0d\u663e\u793a<span class=\"code-snippet__number\">0<\/span>\u7684\u6570\u636e<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e0b\u9762\u662fmissingDataAsZero \u53c2\u6570\u7684\u5b98\u65b9\u89e3\u91ca\uff0c\u5927\u5bb6\u53ef\u4ee5\u8bd5\u7740\u7406\u89e3\u4e00\u4e0b\u55f7<\/span><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"c\"><code><span class=\"code-snippet_outer\">If <span class=\"code-snippet__built_in\">set<\/span>, <span class=\"code-snippet__function\">missing <span class=\"code-snippet__title\">data<\/span> <span class=\"code-snippet__params\">(NAs)<\/span> will be treated as zeros.<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">                        The <span class=\"code-snippet__keyword\">default<\/span> is to ignore such cases, which will be<\/span><\/code><code><span class=\"code-snippet_outer\">                        depicted as black areas in a heatmap. <span class=\"code-snippet__params\">(see the<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">                        --missingDataColor argument of the plotHeatmap command<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet_outer\">                        <span class=\"code-snippet__keyword\">for<\/span> additional options)<\/span>. <span class=\"code-snippet__params\">(<span class=\"code-snippet__keyword\">default<\/span>: False)<\/span><\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4e0b\u9762\u6765\u6b23\u8d4f\u4e00\u4e0b\u5c0f\u679c\u65b0\u7684TSS\u5bcc\u96c6\u70ed\u56fe\u5427<\/span><span style=\"text-align: center;letter-spacing: 0.034em;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u679c\u7136\u6ca1\u6709\u4e86\u9ed1\u7ebf\uff0c\u662f\u4e0d\u662f\u6bd4\u4e4b\u524d\u66f4\u7f8e\u89c2\u4e86\u5462\uff1f<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br  \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u4eca\u5929\u7684\u5173\u4e8eTSS\u5bcc\u96c6\u70ed\u56fe\u7684\u5c0f\u7ec6\u8282\u5b66\u4e60\u5c31\u5230\u8fd9\u91cc\u5566\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u627e\u5c0f\u679c\u8ba8\u8bba\u54e6\uff0c\u6211\u4eec\u660e\u5929\u89c1\u54af~<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-cropselx1=\"0\" data-cropselx2=\"578\" data-cropsely1=\"0\" data-cropsely2=\"322\" data-galleryid=\"\" data-ratio=\"0.7193195625759417\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_oRxs73HBHUoxCtAqBPQnq1AXricRvg-12.png?w=640\" 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