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10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 90px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(255, 229, 231);padding: 9px;\">\n<section powered-by=\"xiumi.us\" style=\"font-size: 18px;text-align: justify;\">\n<p><span style=\"font-size: 17px;\"><strong>\u811a\u672c\u89e3\u8bfb<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 90px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(189, 216, 245);\">\n<section powered-by=\"xiumi.us\" style=\"text-align: center;\">\n<section style=\"font-size: 12px;color: rgb(255, 255, 255);\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: -6px;transform: scale(0.66);transform-origin: left top;\">\n<section style=\"text-align: left;line-height: 1;transform: translate3d(12px, 0px, 0px);\">\n<section style=\"width: 0px;display: inline-block;border-top-width: 0.6em;border-top-style: solid;border-top-color: rgb(247, 221, 223);border-left-width: 0.6em;border-left-style: solid;border-left-color: rgb(247, 221, 223);border-bottom-width: 0.6em !important;border-bottom-style: solid !important;border-bottom-color: transparent !important;border-right-width: 0.6em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">argv <- commandArgs(<span class=\"code-snippet__literal\">TRUE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">in_file <- argv[<span class=\"code-snippet__number\">1<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">map_file <- argv[<span class=\"code-snippet__number\">2<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u91ccargv\u4e3b\u8981\u662f\u53ef\u4ee5\u8bfb\u53d6shell\u4ea4\u4e92\u9875\u9762\u7684\u53c2\u6570\uff0c\u8fd9\u91cc\u7684\u53c2\u6570\u4e3a\u4e24\u4e2a\u6587\u4ef6\uff0c\u7b2c\u4e00\u4e2a\u4e3a\u4e30\u5ea6\u8868\uff0c\u53e6\u5916\u4e00\u4e2a\u4e3a\u5206\u7ec4\u8868<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#read.table\u8bfb\u53d6\u76f8\u5173\u7684\u6570\u636e\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">map <- read.table(map_file,header = <span class=\"code-snippet__built_in\">F<\/span>,<span class=\"code-snippet__built_in\">quote<\/span> = <span class=\"code-snippet__string\">\"\"<\/span>,sep = <span class=\"code-snippet__string\">\"t\"<\/span>,stringsAsFactors = <span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">table <- read.table(in_file,header = <span class=\"code-snippet__built_in\">T<\/span>,sep = <span class=\"code-snippet__string\">\"t\"<\/span>,<span class=\"code-snippet__built_in\">quote<\/span> = <span class=\"code-snippet__string\">\"\"<\/span>,stringsAsFactors = <span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6\u5206\u7ec4\u4fe1\u606f<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">group <- unique(map$V4)<\/span><\/code><code><span class=\"code-snippet_outer\">result <- data.frame(table[,<span class=\"code-snippet__number\">1<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7528\u4e00\u4e2afor\u5faa\u73af\u6765\u8fdb\u884c\u5408\u5e76\uff08\u6ce8\u610f\u8fd9\u91cc\u662f\u6309\u7b2c\u56db\u5217\u5373\u5206\u7ec4\u7684\u5217\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> group){<\/span><\/code><code><span class=\"code-snippet_outer\">samples <- map[which(map$V4==i),][[<span class=\"code-snippet__number\">1<\/span>]]<\/span><\/code><code><span class=\"code-snippet_outer\">tmp_samples <- table[,colnames(table) %in% samples]<\/span><\/code><code><span class=\"code-snippet_outer\">tmp_samples$result <- rowSums(tmp_samples)\/<span class=\"code-snippet__built_in\">length<\/span>(samples)<\/span><\/code><code><span class=\"code-snippet_outer\">result <- cbind(result,tmp_samples$result)<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">##\u8ba1\u7b97\u76f8\u5bf9\u4e30\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colsum <- colSums(result[,-<span class=\"code-snippet__number\">1<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> <span class=\"code-snippet__number\">2<\/span>:ncol(result)){<\/span><\/code><code><span class=\"code-snippet_outer\">result[,i] <- result[,i]\/colsum[[i-<span class=\"code-snippet__number\">1<\/span>]]<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fee\u6539\u4e00\u4e0b\u5217\u540d\uff0c\u5c31\u53ef\u4ee5\u5566\uff5e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(result) <- <span class=\"code-snippet__built_in\">c<\/span>(colnames(table)[<span class=\"code-snippet__number\">1<\/span>],group)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6700\u540e\u518d\u628a\u6587\u4ef6\u8fd4\u5199\u51fa<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">write.table(result,file = <span class=\"code-snippet__string\">\"result.xls\"<\/span>,row.names = <span class=\"code-snippet__built_in\">F<\/span> ,col.names = <span class=\"code-snippet__built_in\">T<\/span>,sep = <span class=\"code-snippet__string\">\"t\"<\/span>, <span class=\"code-snippet__built_in\">quote<\/span> = <span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\">\u6574\u4e2a\u811a\u672c\u867d\u7136\u4ee3\u7801\u4e0d\u591a\uff0c\u4e3b\u8981\u7528\u5230\u4e86for\u5faa\u73af\u6765\u5b9e\u73b0\u7684\u3002<\/p>\n<section powered-by=\"xiumi.us\" style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 108px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(255, 229, 231);padding: 9px;\">\n<section powered-by=\"xiumi.us\" style=\"font-size: 18px;text-align: justify;\">\n<p><span style=\"font-size: 17px;\"><strong>\u811a\u672c\u7684\u7528\u6cd5<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 108px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(189, 216, 245);\">\n<section powered-by=\"xiumi.us\" style=\"text-align: center;\">\n<section style=\"font-size: 12px;color: rgb(255, 255, 255);\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: -6px;transform: scale(0.66);transform-origin: left top;\">\n<section style=\"text-align: left;line-height: 1;transform: translate3d(12px, 0px, 0px);\">\n<section style=\"width: 0px;display: inline-block;border-top-width: 0.6em;border-top-style: solid;border-top-color: rgb(247, 221, 223);border-left-width: 0.6em;border-left-style: solid;border-left-color: rgb(247, 221, 223);border-bottom-width: 0.6em !important;border-bottom-style: solid !important;border-bottom-color: transparent !important;border-right-width: 0.6em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>1\u3001\u8bfb\u8005\u53ef\u4ee5\u4e00\u884c\u884c\u590d\u5236\u5230\u81ea\u5df1R\u73af\u5883\u4e2d\u8fdb\u884c\u6d4b\u8bd5\u64cd\u4f5c\uff0c\u8fd9\u91cc\u6211\u7684\u5206\u7ec4\u6587\u4ef6\u7684\u6709\u6548\u5217\u4e3a\u7b2c\u56db\u5217\uff0c\u6839\u636e\u81ea\u5df1\u7684\u6570\u636e\u5206\u7ec4\u6765\u8c03\u6574\u76f8\u5e94\u7684\u4f4d\u7f6e\u3002<\/p>\n<p><br \/><\/p>\n<p>2\u3001\u8c03\u8282\u597d\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u628a\u8fd9\u4e2a\u811a\u672c\u4fdd\u5b58\u4e00\u4e0b\u4f8b\u5982\u53e6\u5b58\u4e3asamole2group.R\uff0c\u90a3\u4e0b\u6b21\u8fd0\u884c\u65f6\u5019\u53ef\u4ee5\u5728\u7ec8\u7aef\u64cd\u4f5c\u4ee5\u4e0b\u547d\u4ee4Rscript samole2group.R map_file table_file , \u8dd1\u5b8c\u7ed3\u675f\u4f1a\u8fd4\u56de\u540d\u5b57\u4e3aresult.xls\u7684\u7ed3\u679c\u6587\u4ef6\u3002\u67e5\u770b\u6587\u4ef6\u5373\u6309\u7ec4\u5408\u5e76\u597d\u7684\u6587\u4ef6\u3002<\/p>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.07399577167019028\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_CR8lOJzYX21zDnibGFRtRu4xxIMYVA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVHoGh0bPTAxnsLNicYHHjwzUevNqd4BkDKaM0ZVN3sSRiaMmoCR8lOJzYX21zDnibGFRtRu4xxIMYVA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"946\" style=\"vertical-align: middle;width: 0px;\" data-recalc-dims=\"1\" \/><img decoding=\"async\" data-ratio=\"0.11814345991561181\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_eiaqNUlLOrnlhDOYtich45QFKPFMRg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVHoGh0bPTAxnsLNicYHHjwzWtbAXzdvYFGkysdPaN6lUlr97YeiaqNUlLOrnlhDOYtich45QFKPFMRg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"948\" style=\"vertical-align: middle;width: 0px;\" data-recalc-dims=\"1\" \/><img decoding=\"async\" data-ratio=\"0.13425925925925927\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_0AAvlFccxpcmPYlibibYbIdwPnBDAw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVHoGh0bPTAxnsLNicYHHjwzibPsiaHnU3v2qYArAuNswBEiamWJn0AAvlFccxpcmPYlibibYbIdwPnBDAw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 0px;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 76.5px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(255, 229, 231);padding: 9px;\">\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<p><span style=\"font-size: 17px;\"><strong>\u603b\u7ed3<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 76.5px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(189, 216, 245);\">\n<section powered-by=\"xiumi.us\" style=\"text-align: center;\">\n<section style=\"font-size: 12px;color: rgb(255, 255, 255);\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: -6px;transform: scale(0.66);transform-origin: left top;\">\n<section style=\"text-align: left;line-height: 1;transform: translate3d(12px, 0px, 0px);\">\n<section style=\"width: 0px;display: inline-block;border-top-width: 0.6em;border-top-style: solid;border-top-color: rgb(247, 221, 223);border-left-width: 0.6em;border-left-style: solid;border-left-color: rgb(247, 221, 223);border-bottom-width: 0.6em !important;border-bottom-style: solid !important;border-bottom-color: transparent !important;border-right-width: 0.6em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;\">\u5982\u679c\u8bfb\u8005\u719f\u6089shell\u7f16\u7a0b\u8bed\u8a00\uff0c\u4e5f\u53ef\u4ee5\u91c7\u7528\u4e09\u5251\u5ba2\u4e2d\u7684\u4e00\u5458awk\u5b8c\u6210\u6b64\u90e8\u5206\u7684\u64cd\u4f5c\u5e76\u4e14\u64cd\u4f5c\u7b80\u4fbf\uff0c\u4f46\u662f\u9700\u8981\u751f\u4fe1\u5de5\u7a0b\u5e08\u5bf9\u4e8eawk\u7684\u7528\u6cd5\u638c\u63e1\u719f\u7ec3\uff1b\u6b64\u5916\u5982\u679c\u90fd\u89c9\u5f97\u6709\u56f0\u96be\u4e5f\u53ef\u4ee5\u7528excel\u534f\u52a9\u89e3\u51b3\u3002<\/p>\n<p style=\"text-indent: 0em;\"><br \/><\/p>\n<p style=\"text-indent: 0em;\">\u5c0f\u5e08\u59b9\u8ba4\u4e3a\u8fd9\u79cd\u7b80\u5355\u7684\u95ee\u9898\u4e5f\u8bb8\u5c31\u4f1a\u56f0\u6270\u6211\u4eec\u5f88\u4e45\uff08\u5c0f\u5e08\u59b9\u66fe\u7ecf\u4e5f\u5403\u8fc7\u8fd9\u65b9\u9762\u7684\u82e6\uff09\uff0c\u56e0\u6b64\u5efa\u8bae\u5728\u5904\u7406\u95ee\u9898\u7684\u8fc7\u7a0b\u4e2d\u6211\u4eec\u5e94\u8be5\u501f\u52a9\u81ea\u5df1\u6700\u719f\u6089\u7684\u5de5\u5177\u6216\u662f\u7f16\u7a0b\u8bed\u8a00\u6765\u534f\u52a9\u6211\u4eec\u89e3\u51b3\u95ee\u9898\uff0c\u5e76\u4e14\u501f\u9274\u524d\u4eba\u7684\u7ecf\u9a8c\uff0c\u89e3\u51b3\u540e\u4e5f\u9700\u8981\u4fdd\u5b58\u76f8\u5e94\u5199\u597d\u7684\u811a\u672c\u4ee5\u53ca\u811a\u672c\u7684\u4f7f\u7528\u8bf4\u660e\uff0c\u8fd9\u90fd\u662f\u751f\u4fe1\u5c0f\u767d\u5e94\u8be5\u517b\u6210\u7684\u826f\u597d\u7684\u4e60\u60ef\u3002<\/p>\n<p style=\"text-indent: 0em;\"><br \/><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u751f\u4fe1\u5c0f\u5de5\u5177\u6709\u65f6\u5019\u4e5f\u53ef\u4ee5\u8fbe\u5230\u4f60\u60f3\u8981\u7684\u76ee\u7684\u54e6\uff5ehttp:\/\/www.biocloudservice.com\/home.html<\/p>\n<p><br \/><\/p>\n<\/section>\n<p powered-by=\"xiumi.us\" style=\"text-indent: 0em;\">\u4eca\u5929\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\u5566\uff0c\u5982\u679c\u8fd8\u6709\u4ec0\u4e48\u95ee\u9898\u53ef\u4ee5\u548c\u5c0f\u5e08\u59b9\u8fdb\u884c\u8ba8\u8bba\u54e6\uff5e<\/p>\n<p powered-by=\"xiumi.us\" style=\"text-indent: 0em;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_akXKU5NcO1KwuWc1Rk7ZJ56LC2pYrQ-9.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEXjjmsEC5aXz0RE4be2ib1fJPnBD8yX0Z6OF2zocd2ngHpLOakXKU5NcO1KwuWc1Rk7ZJ56LC2pYrQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;justify-content: center;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;align-self: center;flex: 0 0 auto;min-width: 5%;height: auto;margin-right: 20px;margin-left: 20px;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: -3px;\">\n<section style=\"text-align: justify;color: rgb(246, 207, 63);font-size: 20px;\">\n<p style=\"text-align: center;\"><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;margin-bottom: 24px;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br 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10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 90px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(255, 229, 231);padding: 9px;\">\n<section powered-by=\"xiumi.us\" style=\"font-size: 18px;text-align: justify;\">\n<p><span style=\"font-size: 17px;\"><strong>\u811a\u672c\u89e3\u8bfb<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 90px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(189, 216, 245);\">\n<section powered-by=\"xiumi.us\" style=\"text-align: center;\">\n<section style=\"font-size: 12px;color: rgb(255, 255, 255);\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: -6px;transform: scale(0.66);transform-origin: left top;\">\n<section style=\"text-align: left;line-height: 1;transform: translate3d(12px, 0px, 0px);\">\n<section style=\"width: 0px;display: inline-block;border-top-width: 0.6em;border-top-style: solid;border-top-color: rgb(247, 221, 223);border-left-width: 0.6em;border-left-style: solid;border-left-color: rgb(247, 221, 223);border-bottom-width: 0.6em !important;border-bottom-style: solid !important;border-bottom-color: transparent !important;border-right-width: 0.6em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">argv <- commandArgs(<span class=\"code-snippet__literal\">TRUE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">in_file <- argv[<span class=\"code-snippet__number\">1<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">map_file <- argv[<span class=\"code-snippet__number\">2<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8fd9\u91ccargv\u4e3b\u8981\u662f\u53ef\u4ee5\u8bfb\u53d6shell\u4ea4\u4e92\u9875\u9762\u7684\u53c2\u6570\uff0c\u8fd9\u91cc\u7684\u53c2\u6570\u4e3a\u4e24\u4e2a\u6587\u4ef6\uff0c\u7b2c\u4e00\u4e2a\u4e3a\u4e30\u5ea6\u8868\uff0c\u53e6\u5916\u4e00\u4e2a\u4e3a\u5206\u7ec4\u8868<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#read.table\u8bfb\u53d6\u76f8\u5173\u7684\u6570\u636e\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">map <- read.table(map_file,header = <span class=\"code-snippet__built_in\">F<\/span>,<span class=\"code-snippet__built_in\">quote<\/span> = <span class=\"code-snippet__string\">\"\"<\/span>,sep = <span class=\"code-snippet__string\">\"t\"<\/span>,stringsAsFactors = <span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">table <- read.table(in_file,header = <span class=\"code-snippet__built_in\">T<\/span>,sep = <span class=\"code-snippet__string\">\"t\"<\/span>,<span class=\"code-snippet__built_in\">quote<\/span> = <span class=\"code-snippet__string\">\"\"<\/span>,stringsAsFactors = <span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u63d0\u53d6\u5206\u7ec4\u4fe1\u606f<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">group <- unique(map$V4)<\/span><\/code><code><span class=\"code-snippet_outer\">result <- data.frame(table[,<span class=\"code-snippet__number\">1<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7528\u4e00\u4e2afor\u5faa\u73af\u6765\u8fdb\u884c\u5408\u5e76\uff08\u6ce8\u610f\u8fd9\u91cc\u662f\u6309\u7b2c\u56db\u5217\u5373\u5206\u7ec4\u7684\u5217\uff09<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> group){<\/span><\/code><code><span class=\"code-snippet_outer\">samples <- map[which(map$V4==i),][[<span class=\"code-snippet__number\">1<\/span>]]<\/span><\/code><code><span class=\"code-snippet_outer\">tmp_samples <- table[,colnames(table) %in% samples]<\/span><\/code><code><span class=\"code-snippet_outer\">tmp_samples$result <- rowSums(tmp_samples)\/<span class=\"code-snippet__built_in\">length<\/span>(samples)<\/span><\/code><code><span class=\"code-snippet_outer\">result <- cbind(result,tmp_samples$result)<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">##\u8ba1\u7b97\u76f8\u5bf9\u4e30\u5ea6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colsum <- colSums(result[,-<span class=\"code-snippet__number\">1<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">for<\/span>(i <span class=\"code-snippet__keyword\">in<\/span> <span class=\"code-snippet__number\">2<\/span>:ncol(result)){<\/span><\/code><code><span class=\"code-snippet_outer\">result[,i] <- result[,i]\/colsum[[i-<span class=\"code-snippet__number\">1<\/span>]]<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4fee\u6539\u4e00\u4e0b\u5217\u540d\uff0c\u5c31\u53ef\u4ee5\u5566\uff5e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">colnames(result) <- <span class=\"code-snippet__built_in\">c<\/span>(colnames(table)[<span class=\"code-snippet__number\">1<\/span>],group)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u6700\u540e\u518d\u628a\u6587\u4ef6\u8fd4\u5199\u51fa<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">write.table(result,file = <span class=\"code-snippet__string\">\"result.xls\"<\/span>,row.names = <span class=\"code-snippet__built_in\">F<\/span> ,col.names = <span class=\"code-snippet__built_in\">T<\/span>,sep = <span class=\"code-snippet__string\">\"t\"<\/span>, <span class=\"code-snippet__built_in\">quote<\/span> = <span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p powered-by=\"xiumi.us\">\u6574\u4e2a\u811a\u672c\u867d\u7136\u4ee3\u7801\u4e0d\u591a\uff0c\u4e3b\u8981\u7528\u5230\u4e86for\u5faa\u73af\u6765\u5b9e\u73b0\u7684\u3002<\/p>\n<section powered-by=\"xiumi.us\" style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 108px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(255, 229, 231);padding: 9px;\">\n<section powered-by=\"xiumi.us\" style=\"font-size: 18px;text-align: justify;\">\n<p><span style=\"font-size: 17px;\"><strong>\u811a\u672c\u7684\u7528\u6cd5<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 108px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(189, 216, 245);\">\n<section powered-by=\"xiumi.us\" style=\"text-align: center;\">\n<section style=\"font-size: 12px;color: rgb(255, 255, 255);\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: -6px;transform: scale(0.66);transform-origin: left top;\">\n<section style=\"text-align: left;line-height: 1;transform: translate3d(12px, 0px, 0px);\">\n<section style=\"width: 0px;display: inline-block;border-top-width: 0.6em;border-top-style: solid;border-top-color: rgb(247, 221, 223);border-left-width: 0.6em;border-left-style: solid;border-left-color: rgb(247, 221, 223);border-bottom-width: 0.6em !important;border-bottom-style: solid !important;border-bottom-color: transparent !important;border-right-width: 0.6em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>1\u3001\u8bfb\u8005\u53ef\u4ee5\u4e00\u884c\u884c\u590d\u5236\u5230\u81ea\u5df1R\u73af\u5883\u4e2d\u8fdb\u884c\u6d4b\u8bd5\u64cd\u4f5c\uff0c\u8fd9\u91cc\u6211\u7684\u5206\u7ec4\u6587\u4ef6\u7684\u6709\u6548\u5217\u4e3a\u7b2c\u56db\u5217\uff0c\u6839\u636e\u81ea\u5df1\u7684\u6570\u636e\u5206\u7ec4\u6765\u8c03\u6574\u76f8\u5e94\u7684\u4f4d\u7f6e\u3002<\/p>\n<p><br \/><\/p>\n<p>2\u3001\u8c03\u8282\u597d\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u628a\u8fd9\u4e2a\u811a\u672c\u4fdd\u5b58\u4e00\u4e0b\u4f8b\u5982\u53e6\u5b58\u4e3asamole2group.R\uff0c\u90a3\u4e0b\u6b21\u8fd0\u884c\u65f6\u5019\u53ef\u4ee5\u5728\u7ec8\u7aef\u64cd\u4f5c\u4ee5\u4e0b\u547d\u4ee4Rscript samole2group.R map_file table_file , \u8dd1\u5b8c\u7ed3\u675f\u4f1a\u8fd4\u56de\u540d\u5b57\u4e3aresult.xls\u7684\u7ed3\u679c\u6587\u4ef6\u3002\u67e5\u770b\u6587\u4ef6\u5373\u6309\u7ec4\u5408\u5e76\u597d\u7684\u6587\u4ef6\u3002<\/p>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.07399577167019028\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_CR8lOJzYX21zDnibGFRtRu4xxIMYVA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVHoGh0bPTAxnsLNicYHHjwzUevNqd4BkDKaM0ZVN3sSRiaMmoCR8lOJzYX21zDnibGFRtRu4xxIMYVA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"946\" style=\"vertical-align: middle;width: 0px;\" data-recalc-dims=\"1\" \/><img decoding=\"async\" data-ratio=\"0.11814345991561181\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_eiaqNUlLOrnlhDOYtich45QFKPFMRg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVHoGh0bPTAxnsLNicYHHjwzWtbAXzdvYFGkysdPaN6lUlr97YeiaqNUlLOrnlhDOYtich45QFKPFMRg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"948\" style=\"vertical-align: middle;width: 0px;\" data-recalc-dims=\"1\" \/><img decoding=\"async\" data-ratio=\"0.13425925925925927\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_0AAvlFccxpcmPYlibibYbIdwPnBDAw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/oCSOtP61aEVHoGh0bPTAxnsLNicYHHjwzibPsiaHnU3v2qYArAuNswBEiamWJn0AAvlFccxpcmPYlibibYbIdwPnBDAw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 0px;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: center;justify-content: center;display: flex;flex-flow: row nowrap;margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: auto;vertical-align: top;align-self: flex-start;flex: 0 0 auto;min-width: 5%;height: auto;\">\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 76.5px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(255, 229, 231);padding: 9px;\">\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<p><span style=\"font-size: 17px;\"><strong>\u603b\u7ed3<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;width: 76.5px;vertical-align: top;align-self: flex-start;flex: 0 0 auto;background-color: rgb(189, 216, 245);\">\n<section powered-by=\"xiumi.us\" style=\"text-align: center;\">\n<section style=\"font-size: 12px;color: rgb(255, 255, 255);\">\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: -6px;transform: scale(0.66);transform-origin: left top;\">\n<section style=\"text-align: left;line-height: 1;transform: translate3d(12px, 0px, 0px);\">\n<section style=\"width: 0px;display: inline-block;border-top-width: 0.6em;border-top-style: solid;border-top-color: rgb(247, 221, 223);border-left-width: 0.6em;border-left-style: solid;border-left-color: rgb(247, 221, 223);border-bottom-width: 0.6em !important;border-bottom-style: solid !important;border-bottom-color: transparent !important;border-right-width: 0.6em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-indent: 0em;\">\u5982\u679c\u8bfb\u8005\u719f\u6089shell\u7f16\u7a0b\u8bed\u8a00\uff0c\u4e5f\u53ef\u4ee5\u91c7\u7528\u4e09\u5251\u5ba2\u4e2d\u7684\u4e00\u5458awk\u5b8c\u6210\u6b64\u90e8\u5206\u7684\u64cd\u4f5c\u5e76\u4e14\u64cd\u4f5c\u7b80\u4fbf\uff0c\u4f46\u662f\u9700\u8981\u751f\u4fe1\u5de5\u7a0b\u5e08\u5bf9\u4e8eawk\u7684\u7528\u6cd5\u638c\u63e1\u719f\u7ec3\uff1b\u6b64\u5916\u5982\u679c\u90fd\u89c9\u5f97\u6709\u56f0\u96be\u4e5f\u53ef\u4ee5\u7528excel\u534f\u52a9\u89e3\u51b3\u3002<\/p>\n<p style=\"text-indent: 0em;\"><br \/><\/p>\n<p style=\"text-indent: 0em;\">\u5c0f\u5e08\u59b9\u8ba4\u4e3a\u8fd9\u79cd\u7b80\u5355\u7684\u95ee\u9898\u4e5f\u8bb8\u5c31\u4f1a\u56f0\u6270\u6211\u4eec\u5f88\u4e45\uff08\u5c0f\u5e08\u59b9\u66fe\u7ecf\u4e5f\u5403\u8fc7\u8fd9\u65b9\u9762\u7684\u82e6\uff09\uff0c\u56e0\u6b64\u5efa\u8bae\u5728\u5904\u7406\u95ee\u9898\u7684\u8fc7\u7a0b\u4e2d\u6211\u4eec\u5e94\u8be5\u501f\u52a9\u81ea\u5df1\u6700\u719f\u6089\u7684\u5de5\u5177\u6216\u662f\u7f16\u7a0b\u8bed\u8a00\u6765\u534f\u52a9\u6211\u4eec\u89e3\u51b3\u95ee\u9898\uff0c\u5e76\u4e14\u501f\u9274\u524d\u4eba\u7684\u7ecf\u9a8c\uff0c\u89e3\u51b3\u540e\u4e5f\u9700\u8981\u4fdd\u5b58\u76f8\u5e94\u5199\u597d\u7684\u811a\u672c\u4ee5\u53ca\u811a\u672c\u7684\u4f7f\u7528\u8bf4\u660e\uff0c\u8fd9\u90fd\u662f\u751f\u4fe1\u5c0f\u767d\u5e94\u8be5\u517b\u6210\u7684\u826f\u597d\u7684\u4e60\u60ef\u3002<\/p>\n<p style=\"text-indent: 0em;\"><br \/><\/p>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p>\u751f\u4fe1\u5c0f\u5de5\u5177\u6709\u65f6\u5019\u4e5f\u53ef\u4ee5\u8fbe\u5230\u4f60\u60f3\u8981\u7684\u76ee\u7684\u54e6\uff5ehttp:\/\/www.biocloudservice.com\/home.html<\/p>\n<p><br \/><\/p>\n<\/section>\n<p powered-by=\"xiumi.us\" style=\"text-indent: 0em;\">\u4eca\u5929\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\u5566\uff0c\u5982\u679c\u8fd8\u6709\u4ec0\u4e48\u95ee\u9898\u53ef\u4ee5\u548c\u5c0f\u5e08\u59b9\u8fdb\u884c\u8ba8\u8bba\u54e6\uff5e<\/p>\n<p powered-by=\"xiumi.us\" style=\"text-indent: 0em;\"><br \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_akXKU5NcO1KwuWc1Rk7ZJ56LC2pYrQ-9.png?w=640\" 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