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style=\"font-size: 14px;color: rgb(0, 0, 0);\">\u5305\u62ec\u5de5\u5177\u3001\u65b9\u6cd5\u548c\u6570\u636e\u5e93\uff0c\u5e76\u5e26\u6709\u5b58\u50a8\u5e93URL\u3002<\/span><\/span><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5206\u6790\u6d41\u7a0b\u7684\u7b2c\u4e8c\u6b65\u662f\u8d28\u91cf\u63a7\u5236\uff08QC\uff09\uff0c\u4f8b\u5982\u786e\u5b9a\u6bcf\u4e2a\u6761\u5f62\u7801\u7684\u8ba1\u6570\u3001\u57fa\u56e0\u6570\u53ca\u5176\u4e2d\u7ebf\u7c92\u4f53\u57fa\u56e0\u7684\u57fa\u6570\u6bd4\u4f8b\uff0c\u4f4e\u57fa\u56e0\u6570\u548c\u9ad8\u7ebf\u7c92\u4f53\u8bfb\u6570\u6bd4\u4f8b\u901a\u5e38\u8868\u660e\u7ec6\u80de\u8d28\u91cf\u5dee\uff0c\u5728\u8fd9\u91cc\u5c0f\u679c\u63a8\u8350\u51e0\u4e2a\u53cc\u91cd\u68c0\u6d4b\u5de5\u5177\uff0c\u5305\u62ecDoubleDecon, scrulet, DoubletFinder\u7b49\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8781431334622823\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_KAz28vBiamheFuibnpGsnYiay1lzOA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYndk7Gcs4uPNWwZHb79N0VxC0ibiczpKAz28vBiamheFuibnpGsnYiay1lzOA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"517\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"white-space: normal;text-align: center;font-size: 16px;margin-bottom: 0px;letter-spacing: 0.578px;\"><span style=\"font-size: 12px;\">\u56fe 2 \u6570\u636e\u5206\u6790\u7684\u5178\u578b\u6b65\u9aa4<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">\uff082\uff09\u6570\u636e\u6807\u51c6\u5316<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5355\u7ec6\u80de\u6570\u636e\u9700\u8981\u4e0d\u540c\u7c7b\u578b\u548c\u7ea7\u522b\u7684\u5f52\u4e00\u5316\uff0c\u4f8b\u5982\uff0c\u603b\u6d4b\u5e8f\u8bfb\u53d6\u8ba1\u6570\u6570\u6539\u53d8\u539f\u59cb\u8ba1\u6570\u6570\uff0c\u56e0\u6b64\u57fa\u56e0\u8ba1\u6570\u5e94\u6309\u6bd4\u4f8b\u7f29\u653e\u5230\u603b\u4f53\u8ba1\u6570\u6df1\u5ea6\u3002Scran\u4f7f\u7528\u57fa\u4e8e\u6c60\u7684\u5927\u5c0f\u56e0\u5b50\u4f30\u8ba1\u548c\u7ebf\u6027\u56de\u5f52\u5bf9\u6570\u636e\u8fdb\u884c\u5f52\u4e00\u5316\uff0c\u8fd9\u662f\u9664seurat\u4f7f\u7528\u7684\u7b80\u5355\u5bf9\u6570\u5f52\u4e00\u5316\u4e4b\u5916\u6700\u6d41\u884c\u7684\u65b9\u6cd5\u4e4b\u4e00\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 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14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">\uff084\uff09\u53ef\u89c6\u5316\u548c\u805a\u7c7b<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 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style=\"white-space: normal;text-align: center;font-size: 16px;margin-bottom: 0px;letter-spacing: 0.578px;\"><span style=\"font-size: 12px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6344294003868471\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_SiaNG8TBsWCWFsl9icawKvibF9s9HQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYnUkCbAabTuMuuetSRG8C3jItCIEeSiaNG8TBsWCWFsl9icawKvibF9s9HQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"517\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"white-space: normal;text-align: center;font-size: 16px;margin-bottom: 0px;letter-spacing: 0.578px;\"><span style=\"font-size: 12px;\">\u56fe 3 \u6570\u636e\u53ef\u89c6\u5316<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">\uff085\uff09\u7ec6\u80de\u6c34\u5e73\u5206\u6790:<\/span><\/em><\/strong><\/p>\n<p style=\"text-align: center;white-space: normal;\"><span style=\"font-size: 16px;\"><strong><em>\u7ec6\u80de\u5206\u6570\u53d8\u5316\u3001\u5206\u89e3\u548c\u8f68\u8ff9\u5206\u6790<\/em><\/strong><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 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src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_8xV4WhZz0cdib3uFDTxPFAeT7M7asA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYnaMthjHicPPcJF5VviaCJUZkSWFj8xV4WhZz0cdib3uFDTxPFAeT7M7asA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"554\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"white-space: normal;text-align: center;\"><span style=\"font-size: 12px;\">\u56fe 1 scRNA-seq\u5206\u6790\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5de5\u5177<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5c0f\u679c\u5728\u8fd9\u91cc\u4ec5\u5c55\u793a\u90e8\u5206\u76f8\u5173\u5de5\u5177\uff0c\u6709\u5173scRNA-seq\u5206\u6790\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5de5\u5177\u7684\u5168\u9762\u6982\u8ff0\uff0c\u8bf7\u53c2\u89c1https:\/\/www.scrna-tools.org\/tools\u3002<span style=\"font-size: 14px;color: rgb(0, 0, 0);\">\u5305\u62ec\u5de5\u5177\u3001\u65b9\u6cd5\u548c\u6570\u636e\u5e93\uff0c\u5e76\u5e26\u6709\u5b58\u50a8\u5e93URL\u3002<\/span><\/span><\/p>\n<p style=\"white-space: normal;\"><br \/><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5206\u6790\u6d41\u7a0b\u7684\u7b2c\u4e8c\u6b65\u662f\u8d28\u91cf\u63a7\u5236\uff08QC\uff09\uff0c\u4f8b\u5982\u786e\u5b9a\u6bcf\u4e2a\u6761\u5f62\u7801\u7684\u8ba1\u6570\u3001\u57fa\u56e0\u6570\u53ca\u5176\u4e2d\u7ebf\u7c92\u4f53\u57fa\u56e0\u7684\u57fa\u6570\u6bd4\u4f8b\uff0c\u4f4e\u57fa\u56e0\u6570\u548c\u9ad8\u7ebf\u7c92\u4f53\u8bfb\u6570\u6bd4\u4f8b\u901a\u5e38\u8868\u660e\u7ec6\u80de\u8d28\u91cf\u5dee\uff0c\u5728\u8fd9\u91cc\u5c0f\u679c\u63a8\u8350\u51e0\u4e2a\u53cc\u91cd\u68c0\u6d4b\u5de5\u5177\uff0c\u5305\u62ecDoubleDecon, scrulet, DoubletFinder\u7b49\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.8781431334622823\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_KAz28vBiamheFuibnpGsnYiay1lzOA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYndk7Gcs4uPNWwZHb79N0VxC0ibiczpKAz28vBiamheFuibnpGsnYiay1lzOA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"517\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"white-space: normal;text-align: center;font-size: 16px;margin-bottom: 0px;letter-spacing: 0.578px;\"><span style=\"font-size: 12px;\">\u56fe 2 \u6570\u636e\u5206\u6790\u7684\u5178\u578b\u6b65\u9aa4<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">\uff082\uff09\u6570\u636e\u6807\u51c6\u5316<\/span><\/em><\/strong><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">\u5355\u7ec6\u80de\u6570\u636e\u9700\u8981\u4e0d\u540c\u7c7b\u578b\u548c\u7ea7\u522b\u7684\u5f52\u4e00\u5316\uff0c\u4f8b\u5982\uff0c\u603b\u6d4b\u5e8f\u8bfb\u53d6\u8ba1\u6570\u6570\u6539\u53d8\u539f\u59cb\u8ba1\u6570\u6570\uff0c\u56e0\u6b64\u57fa\u56e0\u8ba1\u6570\u5e94\u6309\u6bd4\u4f8b\u7f29\u653e\u5230\u603b\u4f53\u8ba1\u6570\u6df1\u5ea6\u3002Scran\u4f7f\u7528\u57fa\u4e8e\u6c60\u7684\u5927\u5c0f\u56e0\u5b50\u4f30\u8ba1\u548c\u7ebf\u6027\u56de\u5f52\u5bf9\u6570\u636e\u8fdb\u884c\u5f52\u4e00\u5316\uff0c\u8fd9\u662f\u9664seurat\u4f7f\u7528\u7684\u7b80\u5355\u5bf9\u6570\u5f52\u4e00\u5316\u4e4b\u5916\u6700\u6d41\u884c\u7684\u65b9\u6cd5\u4e4b\u4e00\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 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style=\"white-space: normal;text-align: center;font-size: 16px;margin-bottom: 0px;letter-spacing: 0.578px;\"><span style=\"font-size: 12px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6344294003868471\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_SiaNG8TBsWCWFsl9icawKvibF9s9HQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYnUkCbAabTuMuuetSRG8C3jItCIEeSiaNG8TBsWCWFsl9icawKvibF9s9HQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"517\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"white-space: normal;text-align: center;font-size: 16px;margin-bottom: 0px;letter-spacing: 0.578px;\"><span style=\"font-size: 12px;\">\u56fe 3 \u6570\u636e\u53ef\u89c6\u5316<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;white-space: normal;\"><strong><em><span style=\"font-size: 20px;\">\uff085\uff09\u7ec6\u80de\u6c34\u5e73\u5206\u6790:<\/span><\/em><\/strong><\/p>\n<p style=\"text-align: center;white-space: normal;\"><span style=\"font-size: 16px;\"><strong><em>\u7ec6\u80de\u5206\u6570\u53d8\u5316\u3001\u5206\u89e3\u548c\u8f68\u8ff9\u5206\u6790<\/em><\/strong><\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\">Monocle\u662f\u4e00\u79cd\u673a\u5668\u5b66\u4e60\u65b9\u6cd5\uff0c\u7528\u4e8e\u91cd\u5efa\u6bcf\u4e2a\u7ec6\u80de\u4ece\u4e00\u79cd\u72b6\u6001\u8fc7\u6e21\u5230\u53e6\u4e00\u79cd\u72b6\u6001\u65f6\u5fc5\u987b\u6267\u884c\u7684\u57fa\u56e0\u8868\u8fbe\u53d8\u5316\u5e8f\u5217\u3002\u5c0f\u679c\u4e3a\u5927\u5bb6\u63a8\u8350\u4e00\u4e2a\u6709\u7528\u7684\u8f6f\u4ef6\u5305\u662fPHATE\uff0c\u8fd9\u662f\u4e00\u79cd\u5229\u7528\u6570\u636e\u70b9\u4e4b\u95f4\u7684\u4fe1\u606f\u51e0\u4f55\u8ddd\u79bb\u6355\u83b7\u5c40\u90e8\u548c\u5168\u5c40\u975e\u7ebf\u6027\u7ed3\u6784\u7684\u53ef\u89c6\u5316\u65b9\u6cd5\u3002\u63a8\u65ad\u7684\u8f68\u8ff9\u4e0d\u4e00\u5b9a\u8981\u4ee3\u8868\u4e00\u4e2a\u751f\u7269\u8fc7\u7a0b\uff0c\u5e94\u8be5\u6536\u96c6\u8fdb\u4e00\u6b65\u7684\u8bc1\u636e\u6765\u6e90\u6765\u89e3\u91ca\u4ece\u8fd9\u4e9b\u65b9\u6cd5\u4e2d\u5f97\u51fa\u7684\u8f68\u8ff9\u3002<\/span><\/p>\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"><br \/><\/span><\/p>\n<p 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