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sans-serif;color: rgb(36, 41, 47);\">\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7684\u6d41\u7a0b\u5305\u62ec\u6837\u672c\u51c6\u5907\u3001\u82af\u7247\/\u5207\u7247\u5236\u5907\u3001\u6d4b\u5e8f\u548c\u6570\u636e\u5206\u6790\u7b49\u6b65\u9aa4\uff0c\u4e3b\u8981\u5c31\u8981\u5f97\u5230\u56fe\u50cf\u6570\u636e\u548c\u6d4b\u5e8f\u6570\u636e\u3002<\/span><\/span><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;color:rgb(36,41,47);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5050505050505051\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_zE67SaM0mVFndibia9TmrfQ2eskERQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyUib967AtI3cevgHibsGBVJfia18fwpRxrORubYiboibiaP6dscrExzE67SaM0mVFndibia9TmrfQ2eskERQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:Cambria;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:"Times New Roman";font-size:12.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<h1 style=\"text-align: center;\"><\/h1>\n<p><em><span style=\"font-size: 16px;\"><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">1.\u6837\u672c\u51c6\u5907<\/span><\/strong><\/span><\/em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u6837\u672c\u51c6\u5907\u662f\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7814\u7a76\u7684\u7b2c\u4e00\u6b65\uff0c\u5176\u76ee\u7684\u662f\u5c06\u7ec6\u80de\u6216\u7ec4\u7ec7\u6837\u672c\u4fdd\u6301\u5728\u539f\u4f4d\uff0c\u5e76\u4f7fRNA\u4fdd\u6301\u5b8c\u6574\u6027\u548c\u53ef\u9760\u6027\u3002\u4e0d\u540c\u7684\u6837\u672c\u5904\u7406\u65b9\u6cd5\u4f1a\u5f71\u54cd\u6700\u7ec8\u7ed3\u679c\u7684\u51c6\u786e\u6027\u548c\u53ef\u9760\u6027\uff0c\u56e0\u6b64\u9700\u8981\u6839\u636e\u5b9e\u9a8c\u8981\u6c42\u548c\u5177\u4f53\u64cd\u4f5c\u60c5\u51b5\u8fdb\u884c\u9009\u62e9\u3002<o:p><\/o:p><\/span><\/p>\n<p><em><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\">2.\u82af\u7247\/\u5207\u7247\u5236\u5907<\/span><\/strong><\/em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5236\u5907\u82af\u7247\/\u5207\u7247\u662f\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6280\u672f\u7684\u5173\u952e\u6b65\u9aa4\u4e4b\u4e00\u3002\u5bf9\u4e8eST\u6280\u672f\uff0c\u9700\u8981\u5c06\u6837\u672c\u5207\u7247\u540e\u653e\u7f6e\u5728\u7279\u6b8a\u8bbe\u8ba1\u7684\u57fa\u56e0\u82af\u7247\u4e0a\uff0c\u5e76\u5728\u5207\u7247\u4e0a\u52a0\u5165\u5bf9\u4e8eDNA\u7684\u63a2\u9488\uff1b\u5bf9\u4e8eVisium\u6280\u672f\uff0c\u4f7f\u7528\u6807\u51c6\u7684\u56fa\u5b9a\u548c\u67d3\u8272\u6280\u672f\uff0c\u5305\u62ec\u82cf\u6728\u7cbe\u548c\u4f0a\u7ea2(H&E)\u67d3\u8272\uff0c\u4f7f\u7528\u660e\u573a\u663e\u5fae\u955c\u89c2\u5bdf\u8f7d\u73bb\u7247\u4e0a\u7684\u7ec4\u7ec7\u5207\u7247\uff0c\u9700\u8981\u5728\u6837\u672c\u7ec4\u7ec7\u4e2d\u6ce8\u5165\u5305\u542b\u7a7a\u95f4\u6761\u5f62\u7801\u7684\u6c34\u51dd\u80f6\uff0c\u5e76\u5c06\u5176\u56fa\u5b9a\u4e8e\u8f7d\u73bb\u7247\u4e0a\u3002\u8fd9\u6837\u53ef\u4ee5\u5728\u968f\u540e\u7684\u6d4b\u5e8f\u8fc7\u7a0b\u4e2d\u4fdd\u7559\u6bcf\u4e2a\u533a\u57df\u7684\u4f4d\u7f6e\u4fe1\u606f\u3002<o:p><\/o:p><\/span><\/p>\n<p><em><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\">3.\u6d4b\u5e8f<\/span><\/strong><\/em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u6d4b\u5e8f\u662f\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7814\u7a76\u7684\u6838\u5fc3\u6b65\u9aa4\uff0c\u76ee\u7684\u662f\u786e\u5b9a\u6bcf\u4e2a\u4f4d\u7f6e\u4e0a\u7684\u57fa\u56e0\u8868\u8fbe\u4fe1\u606f\u3002\u76ee\u524d\uff0c\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6280\u672f\u5df2\u7ecf\u6210\u4e3a\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7814\u7a76\u4e2d\u4e3b\u8981\u7684\u6d4b\u5e8f\u624b\u6bb5\uff0c\u53ef\u4ee5\u4f7f\u7528Illumina\u7b49\u5546\u4e1a\u6d4b\u5e8f\u5e73\u53f0\u8fdb\u884c\u6d4b\u5e8f\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 16px;\"><em><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">4.\u6570\u636e\u5904\u7406\u548c\u5206\u6790<\/span><\/strong><\/em><\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u6d4b\u5e8f\u5b8c\u6210\u540e\uff0c\u9700\u8981\u5bf9\u6570\u636e\u8fdb\u884c\u5904\u7406\u548c\u5206\u6790\u3002\u8fd9\u5305\u62ec\u4ece\u539f\u59cb\u5e8f\u5217\u6570\u636e\u4e2d\u53bb\u9664\u4f4e\u8d28\u91cf\u7684\u5e8f\u5217\u3001\u5bf9\u5e8f\u5217\u8fdb\u884c\u6bd4\u5bf9\u3001\u8868\u8fbe\u77e9\u9635\u7684\u6784\u5efa\u548c\u91cd\u5efa\u7ec4\u7ec7\u5207\u7247\u56fe\u50cf\u7b49\u6b65\u9aa4\u3002\u6570\u636e\u5904\u7406\u548c\u5206\u6790\u7684\u7ed3\u679c\u53ef\u4ee5\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u53d1\u73b0\u7ec4\u7ec7\u7ed3\u6784\u3001\u7ec6\u80de\u7c7b\u578b\u548c\u76f8\u4e92\u4f5c\u7528\uff0c\u8fdb\u4e00\u6b65\u7406\u89e3\u751f\u7269\u5206\u5b50\u673a\u5236\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5bf9\u4e8e\u6570\u636e\u5206\u6790\uff0c\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u4e5f\u548cscRNA-seq\u7c7b\u4f3c\uff0c\u4e3b\u8981\u5305\u62ec\u6570\u636e\u9884\u5904\u7406\u3001\u964d\u7ef4\u548c\u805a\u7c7b\u3001\u68c0\u6d4b\u7a7a\u95f4\u53d8\u91cf\u7279\u5f81\u7b49\u3002\u73b0\u4f7f\u7528seurat\u8fdb\u884c\u793a\u8303\uff0c\u4ee3\u7801\u5982\u4e0b\uff1a<\/span><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<h1 style=\"text-align: center;\"><em style=\"letter-spacing: 0.034em;\"><\/em><\/h1>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u52a0\u8f7d\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(Seurat)<\/span><\/code><code><span class=\"code-snippet_outer\">library(SeuratData)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(patchwork)<\/span><\/code><code><span class=\"code-snippet_outer\">library(dplyr)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u52a0\u8f7d\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">InstallData(<span class=\"code-snippet__string\">\"stxBrain\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">brain <- LoadData(<span class=\"code-snippet__string\">\"stxBrain\"<\/span>, type = <span class=\"code-snippet__string\">\"anterior1\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u6570\u636e\u9884\u5904\u7406\uff0c\u6807\u51c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">brain <- SCTransform(brain, assay = <span class=\"code-snippet__string\">\"Spatial\"<\/span>, verbose = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">##\u964d\u7ef4\u3001\u805a\u7c7b\u548c\u53ef\u89c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">brain <- RunPCA(brain, assay = <span class=\"code-snippet__string\">\"SCT\"<\/span>, verbose = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">brain <- FindNeighbors(brain, reduction = <span class=\"code-snippet__string\">\"pca\"<\/span>, dims = <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">30<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">brain <- FindClusters(brain, verbose = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">brain <- RunUMAP(brain, reduction = <span class=\"code-snippet__string\">\"pca\"<\/span>, dims = <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">30<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">p1 <- DimPlot(brain, reduction = <span class=\"code-snippet__string\">\"umap\"<\/span>, label = <span class=\"code-snippet__literal\">TRUE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">p2 <- SpatialDimPlot(brain, label = <span class=\"code-snippet__literal\">TRUE<\/span>, label.size = <span class=\"code-snippet__number\">3<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">p1 + p2<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.48328267477203646\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_fsT2icMeMgsCYbLPjT1ibuWZITyiag.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyUib967AtI3cevgHibsGBVJfZiaa0ZgiaKIDgFD9H9a0rW87vcfHFfsT2icMeMgsCYbLPjT1ibuWZITyiag\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"658\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"white-space:pre-wrap;font-family: Consolas, "Liberation Mono", Menlo, Courier, monospace;font-size: 14px;background-color: rgb(250, 250, 250);color: rgb(51, 51, 51);letter-spacing: 0.034em;\">\u200d<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u68c0\u6d4b\u7a7a\u95f4\u53d8\u91cf\u7279\u5f81<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">brain <- FindSpatiallyVariableFeatures(brain, assay = <span class=\"code-snippet__string\">\"SCT\"<\/span>, features = VariableFeatures(brain)[<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">1000<\/span>], selection.method = <span class=\"code-snippet__string\">\"moransi\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 14px;\">\u5c0f\u4f19\u4f34\u4eec\u662f\u4e0d\u662f\u5bf9\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6709\u4e86\u66f4\u591a\u7684\u4e86\u89e3\uff0c\u4e5f\u53ef\u4ee5\u4f7f\u7528\u6211\u4eec\u7684\u5c0f\u5de5\u5177\u8fdb\u884c\u5b9e\u8df5\uff1a<\/span><span style=\"font-size: 14px;\">MIA(multimodal-intersection-analysis)<span style=\"font-size: 14px;\">\u7b97\u6cd5\u6574\u5408\u5355\u7ec6\u80de\u53ca\u7a7a\u95f4\u8f6c\u5f55\u7ec4\uff08<\/span><span style=\"font-size: 14px;\">http:\/\/www.biocloudservice.com\/801\/801.php<\/span><span style=\"font-size: 14px;\">\uff09<\/span><\/span><span style=\"font-size: 14px;\">\uff0c\u5927\u5bb6\u7528\u8d77\u6765\u54e6\u3002<\/span><span style=\"font-size: 14px;\"><o:p><\/o:p><\/span><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u671f\u5f85\u4e0b\u6b21\u4e0e\u60a8\u7684\u76f8\u89c1\u54e6\uff01<\/span><\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u5982\u679c\u5c0f\u4f19\u4f34\u6709\u5176\u4ed6\u6570\u636e\u5206\u6790\u9700\u6c42\uff0c\u53ef\u4ee5\u5c1d\u8bd5\u4f7f\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\u4e91\u5e73\u53f0\uff0c\u96f6\u4ee3\u7801\u5b8c\u6210\u5206\u6790\uff0c\u975e\u5e38\u65b9\u4fbf\u5965\uff0c\u4e91\u5e73\u53f0\u7f51\u5740\u4e3a\uff1a(http:\/\/www.biocloudservice.com\/home.html)\uff0c\u5176\u4e2d\u4e5f\u5305\u62ec\u4e86\u901a\u8def\u8868\u8fbe\u5206\u6790(http:\/\/www.biocloudservice.com\/313\/313.php)\uff0c\u5355\u7ec6\u80de\u7684\u57fa\u56e0\u5171\u8868\u8fbe\u5206\u6790(http:\/\/www.biocloudservice.com\/906\/906.php)\u7b49\u5404\u79cd\u5c0f\u5de5\u5177\u54e6~\uff0c\u6709\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u767b\u5f55\u7f51\u7ad9\u8fdb\u884c\u4e86\u89e3\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 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0.034em;\">\u5404\u4f4d\u5c0f\u4f19\u4f34\u4eec\u597d\u5440\uff0c\u5c0f\u679c\u6765\u55bd\uff01<\/span><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5c0f\u679c\u6700\u8fd1\u5728\u5b66\u4e60\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7684\u6d41\u7a0b\uff0c\u90a3\u4e0b\u9762\u5c31\u7ed9\u5927\u5bb6\u5206\u4eab\u4e00\u4e0b\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u90a3\u6211\u4eec\u4e86\u89e3\u5230\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u662f\u4e00\u79cd\u7814\u7a76\u7ec6\u80de\u6216\u7ec4\u7ec7\u4e2d\u57fa\u56e0\u8868\u8fbe\u7684\u65b9\u6cd5\u3002\u4e0e\u4f20\u7edf\u7684RNA\u6d4b\u5e8f\u6280\u672f\u76f8\u6bd4\uff0c\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5177\u6709\u66f4\u9ad8\u7684\u7a7a\u95f4\u89e3\u6790\u5ea6\uff0c\u56e0\u6b64\u53ef\u4ee5\u76f4\u63a5\u83b7\u5f97\u7ec4\u7ec7\u4e2d\u4e0d\u540c\u7ec6\u80de\u548c\u533a\u57df\u7684\u57fa\u56e0\u8868\u8fbe\u4fe1\u606f\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u76ee\u524d\u5e38\u7528\u7684\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6280\u672f\u5305\u62ecSpatial Transcriptomics\uff08ST\uff09\u548cVisium\u7b49\u5546\u4e1a\u5316\u6280\u672f\u3002\u5176\u4e2d\uff0cST\u5229\u7528\u57fa\u56e0\u82af\u7247\u5c06\u6837\u672c\u4f4d\u7f6e\u4fe1\u606f\u4fdd\u7559\u5728\u82af\u7247\u4e0a\uff0c\u7136\u540e\u901a\u8fc7\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u5bf9RNA\u8fdb\u884c\u6d4b\u5e8f\uff0c\u6700\u7ec8\u751f\u6210\u7ec4\u7ec7\u5207\u7247\u4e0a\u5b8c\u6574\u7684\u57fa\u56e0\u8868\u8fbe\u56fe\u50cf\u3002Visium\u5219\u91c7\u7528\u7a7a\u95f4\u6761\u7801\u6280\u672f\u5bf9\u7ec4\u7ec7\u6837\u672c\u8fdb\u884c\u5206\u79bb\u548c\u6d4b\u5e8f\uff0c\u4ece\u800c\u5f97\u5230\u9ad8\u5206\u8fa8\u7387\u7684\u7a7a\u95f4\u57fa\u56e0\u8868\u8fbe\u56fe\u50cf\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5212\u91cd\u70b9\u5212\u91cd\u70b9\uff0c<span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(36, 41, 47);\">\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7684\u6d41\u7a0b\u5305\u62ec\u6837\u672c\u51c6\u5907\u3001\u82af\u7247\/\u5207\u7247\u5236\u5907\u3001\u6d4b\u5e8f\u548c\u6570\u636e\u5206\u6790\u7b49\u6b65\u9aa4\uff0c\u4e3b\u8981\u5c31\u8981\u5f97\u5230\u56fe\u50cf\u6570\u636e\u548c\u6d4b\u5e8f\u6570\u636e\u3002<\/span><\/span><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;color:rgb(36,41,47);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5050505050505051\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_zE67SaM0mVFndibia9TmrfQ2eskERQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyUib967AtI3cevgHibsGBVJfia18fwpRxrORubYiboibiaP6dscrExzE67SaM0mVFndibia9TmrfQ2eskERQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:Cambria;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:"Times New Roman";font-size:12.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<h1 style=\"text-align: center;\"><\/h1>\n<p><em><span style=\"font-size: 16px;\"><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">1.\u6837\u672c\u51c6\u5907<\/span><\/strong><\/span><\/em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u6837\u672c\u51c6\u5907\u662f\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7814\u7a76\u7684\u7b2c\u4e00\u6b65\uff0c\u5176\u76ee\u7684\u662f\u5c06\u7ec6\u80de\u6216\u7ec4\u7ec7\u6837\u672c\u4fdd\u6301\u5728\u539f\u4f4d\uff0c\u5e76\u4f7fRNA\u4fdd\u6301\u5b8c\u6574\u6027\u548c\u53ef\u9760\u6027\u3002\u4e0d\u540c\u7684\u6837\u672c\u5904\u7406\u65b9\u6cd5\u4f1a\u5f71\u54cd\u6700\u7ec8\u7ed3\u679c\u7684\u51c6\u786e\u6027\u548c\u53ef\u9760\u6027\uff0c\u56e0\u6b64\u9700\u8981\u6839\u636e\u5b9e\u9a8c\u8981\u6c42\u548c\u5177\u4f53\u64cd\u4f5c\u60c5\u51b5\u8fdb\u884c\u9009\u62e9\u3002<o:p><\/o:p><\/span><\/p>\n<p><em><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\">2.\u82af\u7247\/\u5207\u7247\u5236\u5907<\/span><\/strong><\/em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5236\u5907\u82af\u7247\/\u5207\u7247\u662f\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6280\u672f\u7684\u5173\u952e\u6b65\u9aa4\u4e4b\u4e00\u3002\u5bf9\u4e8eST\u6280\u672f\uff0c\u9700\u8981\u5c06\u6837\u672c\u5207\u7247\u540e\u653e\u7f6e\u5728\u7279\u6b8a\u8bbe\u8ba1\u7684\u57fa\u56e0\u82af\u7247\u4e0a\uff0c\u5e76\u5728\u5207\u7247\u4e0a\u52a0\u5165\u5bf9\u4e8eDNA\u7684\u63a2\u9488\uff1b\u5bf9\u4e8eVisium\u6280\u672f\uff0c\u4f7f\u7528\u6807\u51c6\u7684\u56fa\u5b9a\u548c\u67d3\u8272\u6280\u672f\uff0c\u5305\u62ec\u82cf\u6728\u7cbe\u548c\u4f0a\u7ea2(H&E)\u67d3\u8272\uff0c\u4f7f\u7528\u660e\u573a\u663e\u5fae\u955c\u89c2\u5bdf\u8f7d\u73bb\u7247\u4e0a\u7684\u7ec4\u7ec7\u5207\u7247\uff0c\u9700\u8981\u5728\u6837\u672c\u7ec4\u7ec7\u4e2d\u6ce8\u5165\u5305\u542b\u7a7a\u95f4\u6761\u5f62\u7801\u7684\u6c34\u51dd\u80f6\uff0c\u5e76\u5c06\u5176\u56fa\u5b9a\u4e8e\u8f7d\u73bb\u7247\u4e0a\u3002\u8fd9\u6837\u53ef\u4ee5\u5728\u968f\u540e\u7684\u6d4b\u5e8f\u8fc7\u7a0b\u4e2d\u4fdd\u7559\u6bcf\u4e2a\u533a\u57df\u7684\u4f4d\u7f6e\u4fe1\u606f\u3002<o:p><\/o:p><\/span><\/p>\n<p><em><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\">3.\u6d4b\u5e8f<\/span><\/strong><\/em><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u6d4b\u5e8f\u662f\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7814\u7a76\u7684\u6838\u5fc3\u6b65\u9aa4\uff0c\u76ee\u7684\u662f\u786e\u5b9a\u6bcf\u4e2a\u4f4d\u7f6e\u4e0a\u7684\u57fa\u56e0\u8868\u8fbe\u4fe1\u606f\u3002\u76ee\u524d\uff0c\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6280\u672f\u5df2\u7ecf\u6210\u4e3a\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7814\u7a76\u4e2d\u4e3b\u8981\u7684\u6d4b\u5e8f\u624b\u6bb5\uff0c\u53ef\u4ee5\u4f7f\u7528Illumina\u7b49\u5546\u4e1a\u6d4b\u5e8f\u5e73\u53f0\u8fdb\u884c\u6d4b\u5e8f\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 16px;\"><em><strong><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">4.\u6570\u636e\u5904\u7406\u548c\u5206\u6790<\/span><\/strong><\/em><\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u6d4b\u5e8f\u5b8c\u6210\u540e\uff0c\u9700\u8981\u5bf9\u6570\u636e\u8fdb\u884c\u5904\u7406\u548c\u5206\u6790\u3002\u8fd9\u5305\u62ec\u4ece\u539f\u59cb\u5e8f\u5217\u6570\u636e\u4e2d\u53bb\u9664\u4f4e\u8d28\u91cf\u7684\u5e8f\u5217\u3001\u5bf9\u5e8f\u5217\u8fdb\u884c\u6bd4\u5bf9\u3001\u8868\u8fbe\u77e9\u9635\u7684\u6784\u5efa\u548c\u91cd\u5efa\u7ec4\u7ec7\u5207\u7247\u56fe\u50cf\u7b49\u6b65\u9aa4\u3002\u6570\u636e\u5904\u7406\u548c\u5206\u6790\u7684\u7ed3\u679c\u53ef\u4ee5\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u53d1\u73b0\u7ec4\u7ec7\u7ed3\u6784\u3001\u7ec6\u80de\u7c7b\u578b\u548c\u76f8\u4e92\u4f5c\u7528\uff0c\u8fdb\u4e00\u6b65\u7406\u89e3\u751f\u7269\u5206\u5b50\u673a\u5236\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5bf9\u4e8e\u6570\u636e\u5206\u6790\uff0c\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u4e5f\u548cscRNA-seq\u7c7b\u4f3c\uff0c\u4e3b\u8981\u5305\u62ec\u6570\u636e\u9884\u5904\u7406\u3001\u964d\u7ef4\u548c\u805a\u7c7b\u3001\u68c0\u6d4b\u7a7a\u95f4\u53d8\u91cf\u7279\u5f81\u7b49\u3002\u73b0\u4f7f\u7528seurat\u8fdb\u884c\u793a\u8303\uff0c\u4ee3\u7801\u5982\u4e0b\uff1a<\/span><span style=\"mso-spacerun:'yes';font-family:'Times New Roman';mso-fareast-font-family:\u5b8b\u4f53;font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<h1 style=\"text-align: center;\"><em style=\"letter-spacing: 0.034em;\"><\/em><\/h1>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u52a0\u8f7d\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(Seurat)<\/span><\/code><code><span class=\"code-snippet_outer\">library(SeuratData)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(patchwork)<\/span><\/code><code><span class=\"code-snippet_outer\">library(dplyr)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u52a0\u8f7d\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">InstallData(<span class=\"code-snippet__string\">\"stxBrain\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">brain <- LoadData(<span class=\"code-snippet__string\">\"stxBrain\"<\/span>, type = <span class=\"code-snippet__string\">\"anterior1\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u6570\u636e\u9884\u5904\u7406\uff0c\u6807\u51c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">brain <- SCTransform(brain, assay = <span class=\"code-snippet__string\">\"Spatial\"<\/span>, verbose = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">##\u964d\u7ef4\u3001\u805a\u7c7b\u548c\u53ef\u89c6\u5316<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">brain <- RunPCA(brain, assay = <span class=\"code-snippet__string\">\"SCT\"<\/span>, verbose = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">brain <- FindNeighbors(brain, reduction = <span class=\"code-snippet__string\">\"pca\"<\/span>, dims = <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">30<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">brain <- FindClusters(brain, verbose = <span class=\"code-snippet__literal\">FALSE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">brain <- RunUMAP(brain, reduction = <span class=\"code-snippet__string\">\"pca\"<\/span>, dims = <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">30<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">p1 <- DimPlot(brain, reduction = <span class=\"code-snippet__string\">\"umap\"<\/span>, label = <span class=\"code-snippet__literal\">TRUE<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">p2 <- SpatialDimPlot(brain, label = <span class=\"code-snippet__literal\">TRUE<\/span>, label.size = <span class=\"code-snippet__number\">3<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">p1 + p2<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.48328267477203646\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_fsT2icMeMgsCYbLPjT1ibuWZITyiag.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyUib967AtI3cevgHibsGBVJfZiaa0ZgiaKIDgFD9H9a0rW87vcfHFfsT2icMeMgsCYbLPjT1ibuWZITyiag\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"658\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"white-space:pre-wrap;font-family: Consolas, "Liberation Mono", Menlo, Courier, monospace;font-size: 14px;background-color: rgb(250, 250, 250);color: rgb(51, 51, 51);letter-spacing: 0.034em;\">\u200d<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u68c0\u6d4b\u7a7a\u95f4\u53d8\u91cf\u7279\u5f81<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">brain <- FindSpatiallyVariableFeatures(brain, assay = <span class=\"code-snippet__string\">\"SCT\"<\/span>, features = VariableFeatures(brain)[<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">1000<\/span>], selection.method = <span class=\"code-snippet__string\">\"moransi\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 14px;\">\u5c0f\u4f19\u4f34\u4eec\u662f\u4e0d\u662f\u5bf9\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6709\u4e86\u66f4\u591a\u7684\u4e86\u89e3\uff0c\u4e5f\u53ef\u4ee5\u4f7f\u7528\u6211\u4eec\u7684\u5c0f\u5de5\u5177\u8fdb\u884c\u5b9e\u8df5\uff1a<\/span><span style=\"font-size: 14px;\">MIA(multimodal-intersection-analysis)<span style=\"font-size: 14px;\">\u7b97\u6cd5\u6574\u5408\u5355\u7ec6\u80de\u53ca\u7a7a\u95f4\u8f6c\u5f55\u7ec4\uff08<\/span><span style=\"font-size: 14px;\">http:\/\/www.biocloudservice.com\/801\/801.php<\/span><span style=\"font-size: 14px;\">\uff09<\/span><\/span><span 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sans-serif;\">\u5982\u679c\u5c0f\u4f19\u4f34\u6709\u5176\u4ed6\u6570\u636e\u5206\u6790\u9700\u6c42\uff0c\u53ef\u4ee5\u5c1d\u8bd5\u4f7f\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\u4e91\u5e73\u53f0\uff0c\u96f6\u4ee3\u7801\u5b8c\u6210\u5206\u6790\uff0c\u975e\u5e38\u65b9\u4fbf\u5965\uff0c\u4e91\u5e73\u53f0\u7f51\u5740\u4e3a\uff1a(http:\/\/www.biocloudservice.com\/home.html)\uff0c\u5176\u4e2d\u4e5f\u5305\u62ec\u4e86\u901a\u8def\u8868\u8fbe\u5206\u6790(http:\/\/www.biocloudservice.com\/313\/313.php)\uff0c\u5355\u7ec6\u80de\u7684\u57fa\u56e0\u5171\u8868\u8fbe\u5206\u6790(http:\/\/www.biocloudservice.com\/906\/906.php)\u7b49\u5404\u79cd\u5c0f\u5de5\u5177\u54e6~\uff0c\u6709\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u767b\u5f55\u7f51\u7ad9\u8fdb\u884c\u4e86\u89e3\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 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\/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6574\u4f53\u6d41\u7a0b\u662f\u4ec0\u4e48\uff1f\u5c0f\u679c\u6765\u4e3a\u60a8\u89e3\u60d1 \u539f\u521b \u5c0f\u679c \u751f\u4fe1\u679c&nb 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