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powered-by=\"xiumi.us\">\n<p>\u7bb1\u7ebf\u56fe\uff08Boxplot\uff09\u662f\u4e00\u79cd\u5e38\u7528\u7684\u7edf\u8ba1\u56fe\u5f62\uff0c\u7528\u4e8e\u663e\u793a\u6570\u636e\u96c6\u7684\u5206\u5e03\u7279\u5f81\u548c\u79bb\u7fa4\u503c\u60c5\u51b5\u3002\u5b83\u901a\u8fc7\u4e94\u4e2a\u7edf\u8ba1\u91cf\uff08\u6700\u5c0f\u503c\u3001\u4e0b\u56db\u5206\u4f4d\u6570\u3001\u4e2d\u4f4d\u6570\u3001\u4e0a\u56db\u5206\u4f4d\u6570\u3001\u6700\u5927\u503c\uff09\u6765\u63cf\u8ff0\u6570\u636e\u7684\u4f4d\u7f6e\u548c\u79bb\u6563\u7a0b\u5ea6\u3002<\/p>\n<p>\u4e0b\u9762\u6765\u770b\u4ee3\u7801\uff0c\u8fd9\u662f\u793a\u4f8b\u6570\u636e\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.8824074074074074\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_WHiarq7vWibic5ia9iahlKzxAXyQXg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOs92DwIqqBwVY0VRaWYaQk8vPw0h59WHiarq7vWibic5ia9iahlKzxAXyQXg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#install.packages(\"ggpubr\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr) <span class=\"code-snippet__comment\">#\u5f15\u7528\u5305library(ggpubr)\u52a0\u8f7dggpubr\u5305\uff0c\u8be5\u5305\u63d0\u4f9b\u4e86\u7528\u4e8e\u7ed8\u5236\u7bb1\u578b\u56fe\u548c\u7edf\u8ba1\u5206\u6790\u7684\u51fd\u6570\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">inputFile=<span class=\"code-snippet__string\">\"geneExp.txt\"<\/span> <span class=\"code-snippet__comment\">#\u8f93\u5165\u6587\u4ef6\u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">\"C:\\biowolf\\geneCeRNA\\08.panDiff\"<\/span>) <span class=\"code-snippet__comment\">#\u4f7f\u7528setwd()\u51fd\u6570\u8bbe\u7f6e\u5de5\u4f5c\u76ee\u5f55\uff0c\u5373\u6570\u636e\u6587\u4ef6\u6240\u5728\u7684\u76ee\u5f55<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6\u8f93\u5165\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=read.table(inputFile, header=<span class=\"code-snippet__built_in\">T<\/span>, sep=<span class=\"code-snippet__string\">\"t\"<\/span>, check.names=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9648148148148148\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_boW8BLicXQjFQcNgtbCgTiaiaUCQFA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOvEeDcJtKdBBeibC5pzClv5icJw00rboW8BLicXQjFQcNgtbCgTiaiaUCQFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u6587\u4ef6\uff0c\u5e76\u6839\u636e\u764c\u75c7\u7c7b\u578b\u8fdb\u884c\u5206\u7ec4\uff0c\u7136\u540e\u7ed8\u5236\u7bb1\u578b\u56fe\u6765\u5c55\u793a\u4e0d\u540c\u764c\u75c7\u7c7b\u578b\u4e2d\u57fa\u56e0\u8868\u8fbe\u7684\u5dee\u5f02\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">gene=colnames(data)[<span class=\"code-snippet__number\">2<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">colnames(data)[<span class=\"code-snippet__number\">2<\/span>]=<span class=\"code-snippet__string\">\"expression\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">##gene\u53d8\u91cf\u5b58\u50a8\u57fa\u56e0\u540d\uff0ccolnames(data)[2]\u5c06\u7b2c\u4e8c\u5217\u7684\u5217\u540d\uff08\u57fa\u56e0\u8868\u8fbe\u503c\uff09\u66f4\u6539\u4e3a\"expression\"\u3002\u7edf\u8ba1\u6bcf\u4e2a\u80bf\u7624\u6b63\u5e38\u6837\u54c1\u6570\u76ee\uff1a\u4ece\u6570\u636e\u6846\u4e2d\u9009\u62e9\u6b63\u5e38\u6837\u54c1\uff0c\u5e76\u4f7f\u7528table()\u51fd\u6570\u7edf\u8ba1\u6bcf\u4e2a\u80bf\u7624\u7c7b\u578b\u7684\u6837\u54c1\u6570\u76ee\u3002NormalNum\u4fdd\u5b58\u6837\u54c1\u6570\u76ee\u5927\u4e8e\u7b49\u4e8e5\u7684\u80bf\u7624\u7c7b\u578b\u3002NormalCancer\u4fdd\u5b58\u6837\u54c1\u6570\u76ee\u5927\u4e8e\u7b49\u4e8e5\u7684\u80bf\u7624\u7c7b\u578b\u7684\u540d\u79f0\u3002\u6570\u636e\u7b5b\u9009\uff1a\u4f7f\u7528which()\u51fd\u6570\u548c%in%\u64cd\u4f5c\u7b26\uff0c\u4ece\u6570\u636e\u6846\u4e2d\u9009\u62e9\u5c5e\u4e8eNormalCancer\u4e2d\u80bf\u7624\u7c7b\u578b\u7684\u6570\u636e\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7edf\u8ba1\u6bcf\u4e2a\u80bf\u7624\u6b63\u5e38\u6837\u54c1\u6570\u76ee,\u6311\u9009\u6b63\u5e38\u6837\u54c1\u6570\u76ee>=5\u7684\u80bf\u7624<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">Normal=data[data[,<span class=\"code-snippet__string\">\"Type\"<\/span>]==<span class=\"code-snippet__string\">\"Normal\"<\/span>,]<\/span><\/code><code><span class=\"code-snippet_outer\">NormalNum=table(Normal[,<span class=\"code-snippet__string\">\"CancerType\"<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\">NormalNum=NormalNum[NormalNum>=<span class=\"code-snippet__number\">5<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">NormalCacner=<span class=\"code-snippet__built_in\">names<\/span>(NormalNum)<\/span><\/code><code><span class=\"code-snippet_outer\">data=data[which(data[,<span class=\"code-snippet__string\">\"CancerType\"<\/span>] %in% NormalCacner),]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u7bb1\u578b\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p=ggboxplot(data, x=<span class=\"code-snippet__string\">\"CancerType\"<\/span>, y=<span class=\"code-snippet__string\">\"expression\"<\/span>, color=<span class=\"code-snippet__string\">\"Type\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab=<span class=\"code-snippet__string\">\"\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> ylab=paste0(gene,<span class=\"code-snippet__string\">\" expression\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> palette = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"blue\"<\/span>,<span class=\"code-snippet__string\">\"red\"<\/span>) )<\/span><\/code><code><span class=\"code-snippet_outer\">p=p+rotate_x_text(<span class=\"code-snippet__number\">60<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u7bb1\u578b\u56fe\uff1a\u4f7f\u7528ggboxplot()\u51fd\u6570\u7ed8\u5236\u7bb1\u578b\u56fe\u3002x=\"CancerType\"\u6307\u5b9ax\u8f74\u4e3a\u764c\u75c7\u7c7b\u578b\uff0cy=\"expression\"\u6307\u5b9ay\u8f74\u4e3a\u57fa\u56e0\u8868\u8fbe\u503c\u3002color=\"Type\"\u6307\u5b9a\u6839\u636e\u6837\u54c1\u7c7b\u578b\uff08\u6b63\u5e38\/\u80bf\u7624\uff09\u8fdb\u884c\u989c\u8272\u533a\u5206\u3002xlab=\"\"\u5c06x\u8f74\u6807\u7b7e\u8bbe\u7f6e\u4e3a\u7a7a\uff0cylab=paste0(gene,\" expression\")\u8bbe\u7f6ey\u8f74\u6807\u7b7e\u4e3a\u57fa\u56e0\u540d + \"expression\"\u3002palette = c(\"blue\",\"red\")\u8bbe\u7f6e\u989c\u8272\u65b9\u6848\uff0c\u84dd\u8272\u8868\u793a\u6b63\u5e38\u6837\u54c1\uff0c\u7ea2\u8272\u8868\u793a\u80bf\u7624\u6837\u54c1\u3002rotate_x_text(60)\u5c06x\u8f74\u6807\u7b7e\u65cb\u8f6c60\u5ea6\uff0c\u4ee5\u907f\u514d\u6807\u7b7e\u91cd\u53e0\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u56fe\u5f62<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pdf(file=<span class=\"code-snippet__string\">\"boxplot.pdf\"<\/span>, width=<span class=\"code-snippet__number\">7.5<\/span>, height=<span class=\"code-snippet__number\">5<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">p+stat_compare_means(aes(group=Type),<\/span><\/code><code><span class=\"code-snippet_outer\"> method=<span class=\"code-snippet__string\">\"wilcox.test\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7edf\u8ba1\u5206\u6790\u65b9\u6cd5\uff08wilcox.test\uff09\u6765\u6bd4\u8f83\u4e0d\u540c\u7c7b\u578b\u4e4b\u95f4\u7684\u5dee\u5f02<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> symnum.args=<span class=\"code-snippet__built_in\">list<\/span>(cutpoints = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">0<\/span>, <span class=\"code-snippet__number\">0.001<\/span>, <span class=\"code-snippet__number\">0.01<\/span>, <span class=\"code-snippet__number\">0.05<\/span>, <span class=\"code-snippet__number\">1<\/span>), symbols = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"***\"<\/span>, <span class=\"code-snippet__string\">\"**\"<\/span>, <span class=\"code-snippet__string\">\"*\"<\/span>, <span class=\"code-snippet__string\">\" \"<\/span>)), <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u56fe\u4e2d\u6807\u6ce8\u663e\u8457\u6027\u6c34\u5e73<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">###\u8bbe\u7f6e\u8f93\u51fa\u56fe\u5f62\u7684\u5927\u5c0f\u3002stat_compare_means()\u51fd\u6570\u4e8e\u5728\u7bb1\u578b\u56fe\u4e0a\u8fdb\u884c\u7edf\u8ba1\u5206\u6790\uff0c\u6807\u6ce8\u4e0d\u540c\u764c\u75c7\u7c7b\u578b\u4e4b\u95f4\u7684\u663e\u8457\u6027\u5dee\u5f02\u3002aes(group=Type)\u6307\u5b9a\u8fdb\u884c\u6bd4\u8f83\u7684\u5206\u7ec4\u53d8\u91cf\u4e3a\u6837\u54c1\u7c7b\u578b\uff08\u6b63\u5e38\/\u80bf\u7624\uff09\u3002method=\"wilcox.test\"\u4f7f\u7528Wilcoxon\u79e9\u548c\u68c0\u9a8c\u8fdb\u884c\u6bd4\u8f83\u3002symnum.args\u53c2\u6570\u5b9a\u4e49\u4e86\u663e\u8457\u6027\u6c34\u5e73\u7684\u7b26\u53f7\u8868\u793a\u3002label=\"p.signif\"\u6807\u6ce8\u663e\u8457\u6027\u6c34\u5e73\u3002dev.off()\u5173\u95edpdf\u8f93\u51fa\u3002<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>\u6765\u7ed9\u5927\u5bb6\u5c55\u793a\u5c55\u793a\u7ed3\u679c\u56fe\u5427\uff0c\u4f7f\u7528firefox\u547d\u4ee4\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.6509259259259259\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_DiaTHcAUf7DbCJlsrZib1lPsozd8HA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOMARJS9D7PTM7GeGoOlmaK198SbDiaTHcAUf7DbCJlsrZib1lPsozd8HA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p>\u6839\u636e\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u7ed8\u5236\u7bb1\u578b\u56fe\uff0c\u5c55\u793a\u4e0d\u540c\u764c\u75c7\u7c7b\u578b\u4e2d\u57fa\u56e0\u8868\u8fbe\u7684\u5dee\u5f02\uff0c\u5e76\u901a\u8fc7\u7edf\u8ba1\u5206\u6790\u65b9\u6cd5\u6bd4\u8f83\u4e0d\u540c\u764c\u75c7\u7c7b\u578b\u4e4b\u95f4\u7684\u663e\u8457\u6027\u5dee\u5f02\u3002\u8fd9\u53ef\u4ee5\u5e2e\u52a9\u4e86\u89e3\u4e0d\u540c\u764c\u75c7\u7c7b\u578b\u4e2d\u57fa\u56e0\u8868\u8fbe\u7684\u53d8\u5316\uff0c\u5e76\u53d1\u73b0\u53ef\u80fd\u4e0e\u764c\u75c7\u53d1\u5c55\u76f8\u5173\u7684\u57fa\u56e0\u3002<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 1px;padding-right: 10px;padding-left: 10px;\" 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115);font-size: 19px;padding-right: 11px;padding-left: 11px;line-height: 1;text-align: justify;\">\n<p style=\"text-align: left;text-wrap: wrap;\"><strong>\u70b9\u51fb\u84dd\u5b57 \u5173\u6ce8\u6211\u4eec<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;line-height: 0;flex: 0 0 0%;height: auto;\">\n<section style=\"text-align: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(59, 149, 115);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 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\/><\/p>\n<p>\u6cdb\u764c\u5206\u6790\u662f\u5bf9\u591a\u79cd\u764c\u75c7\u7c7b\u578b\u8fdb\u884c\u6574\u4f53\u5206\u6790\u548c\u6bd4\u8f83\u7684\u7814\u7a76\u65b9\u6cd5\u3002\u7efc\u5408\u8003\u8651\u591a\u79cd\u764c\u75c7\u7c7b\u578b\u7684\u5171\u6027\u548c\u5dee\u5f02\uff0c\u63ed\u793a\u51fa\u764c\u75c7\u53d1\u5c55\u7684\u4e00\u822c\u6027\u89c4\u5f8b\u548c\u751f\u7269\u5b66\u673a\u5236\u3002\u53d1\u73b0\u6f5c\u5728\u9776\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u5bf9\u591a\u4e2a\u764c\u75c7\u7c7b\u578b\u7684\u57fa\u56e0\u8868\u8fbe\u3001\u7a81\u53d8\u3001\u878d\u5408\u7b49\u4fe1\u606f\u8fdb\u884c\u7efc\u5408\u5206\u6790\uff0c\u53ef\u4ee5\u53d1\u73b0\u5171\u540c\u7684\u5f02\u5e38\u4fe1\u53f7\u548c\u9776\u6807\uff0c\u8fd9\u4e9b\u9776\u6807\u53ef\u80fd\u5728\u591a\u79cd\u764c\u75c7\u7c7b\u578b\u4e2d\u8d77\u5230\u91cd\u8981\u4f5c\u7528\u3002\u8fd9\u6709\u52a9\u4e8e\u53d1\u73b0\u6f5c\u5728\u7684\u6cbb\u7597\u9776\u70b9\u548c\u9884\u540e\u6807\u5fd7\u7269\uff0c\u63d0\u4f9b\u66f4\u5e7f\u6cdb\u7684\u6cbb\u7597\u9009\u62e9\u548c\u4e2a\u4f53\u5316\u6cbb\u7597\u7b56\u7565\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u8bc6\u522b\u764c\u75c7\u9a71\u52a8\u57fa\u56e0\uff1a\u6cdb\u764c\u5206\u6790\u53ef\u4ee5\u5e2e\u52a9\u8bc6\u522b\u5728\u591a\u79cd\u764c\u75c7\u7c7b\u578b\u4e2d\u9ad8\u5ea6\u5171\u6709\u7684\u764c\u75c7\u9a71\u52a8\u57fa\u56e0\u3002\u8fd9\u4e9b\u57fa\u56e0\u5bf9\u4e8e\u764c\u75c7\u53d1\u5c55\u548c\u8fdb\u5c55\u8d77\u5230\u5173\u952e\u4f5c\u7528\uff0c\u56e0\u6b64\u6210\u4e3a\u91cd\u8981\u7684\u6cbb\u7597\u76ee\u6807\u548c\u7814\u7a76\u5bf9\u8c61\u3002\u901a\u8fc7\u5bf9\u8fd9\u4e9b\u57fa\u56e0\u7684\u6df1\u5165\u7814\u7a76\uff0c\u53ef\u4ee5\u66f4\u597d\u5730\u7406\u89e3\u764c\u75c7\u7684\u53d1\u751f\u673a\u5236\uff0c\u5e76\u4e3a\u764c\u75c7\u6cbb\u7597\u63d0\u4f9b\u65b0\u7684\u9776\u5411\u6cbb\u7597\u7b56\u7565\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u7bb1\u7ebf\u56fe\uff08Boxplot\uff09\u662f\u4e00\u79cd\u5e38\u7528\u7684\u7edf\u8ba1\u56fe\u5f62\uff0c\u7528\u4e8e\u663e\u793a\u6570\u636e\u96c6\u7684\u5206\u5e03\u7279\u5f81\u548c\u79bb\u7fa4\u503c\u60c5\u51b5\u3002\u5b83\u901a\u8fc7\u4e94\u4e2a\u7edf\u8ba1\u91cf\uff08\u6700\u5c0f\u503c\u3001\u4e0b\u56db\u5206\u4f4d\u6570\u3001\u4e2d\u4f4d\u6570\u3001\u4e0a\u56db\u5206\u4f4d\u6570\u3001\u6700\u5927\u503c\uff09\u6765\u63cf\u8ff0\u6570\u636e\u7684\u4f4d\u7f6e\u548c\u79bb\u6563\u7a0b\u5ea6\u3002<\/p>\n<p>\u4e0b\u9762\u6765\u770b\u4ee3\u7801\uff0c\u8fd9\u662f\u793a\u4f8b\u6570\u636e\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" data-ratio=\"0.8824074074074074\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_WHiarq7vWibic5ia9iahlKzxAXyQXg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOs92DwIqqBwVY0VRaWYaQk8vPw0h59WHiarq7vWibic5ia9iahlKzxAXyQXg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#install.packages(\"ggpubr\")<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr) <span class=\"code-snippet__comment\">#\u5f15\u7528\u5305library(ggpubr)\u52a0\u8f7dggpubr\u5305\uff0c\u8be5\u5305\u63d0\u4f9b\u4e86\u7528\u4e8e\u7ed8\u5236\u7bb1\u578b\u56fe\u548c\u7edf\u8ba1\u5206\u6790\u7684\u51fd\u6570\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">inputFile=<span class=\"code-snippet__string\">\"geneExp.txt\"<\/span> <span class=\"code-snippet__comment\">#\u8f93\u5165\u6587\u4ef6\u540d\u79f0<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">\"C:\\biowolf\\geneCeRNA\\08.panDiff\"<\/span>) <span class=\"code-snippet__comment\">#\u4f7f\u7528setwd()\u51fd\u6570\u8bbe\u7f6e\u5de5\u4f5c\u76ee\u5f55\uff0c\u5373\u6570\u636e\u6587\u4ef6\u6240\u5728\u7684\u76ee\u5f55<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6\u8f93\u5165\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data=read.table(inputFile, header=<span class=\"code-snippet__built_in\">T<\/span>, sep=<span class=\"code-snippet__string\">\"t\"<\/span>, check.names=<span class=\"code-snippet__built_in\">F<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.9648148148148148\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_boW8BLicXQjFQcNgtbCgTiaiaUCQFA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSSYqN0u6bwwXzLCBdlX9BXOvEeDcJtKdBBeibC5pzClv5icJw00rboW8BLicXQjFQcNgtbCgTiaiaUCQFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8bfb\u53d6\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u6587\u4ef6\uff0c\u5e76\u6839\u636e\u764c\u75c7\u7c7b\u578b\u8fdb\u884c\u5206\u7ec4\uff0c\u7136\u540e\u7ed8\u5236\u7bb1\u578b\u56fe\u6765\u5c55\u793a\u4e0d\u540c\u764c\u75c7\u7c7b\u578b\u4e2d\u57fa\u56e0\u8868\u8fbe\u7684\u5dee\u5f02\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">gene=colnames(data)[<span class=\"code-snippet__number\">2<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">colnames(data)[<span class=\"code-snippet__number\">2<\/span>]=<span class=\"code-snippet__string\">\"expression\"<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">##gene\u53d8\u91cf\u5b58\u50a8\u57fa\u56e0\u540d\uff0ccolnames(data)[2]\u5c06\u7b2c\u4e8c\u5217\u7684\u5217\u540d\uff08\u57fa\u56e0\u8868\u8fbe\u503c\uff09\u66f4\u6539\u4e3a\"expression\"\u3002\u7edf\u8ba1\u6bcf\u4e2a\u80bf\u7624\u6b63\u5e38\u6837\u54c1\u6570\u76ee\uff1a\u4ece\u6570\u636e\u6846\u4e2d\u9009\u62e9\u6b63\u5e38\u6837\u54c1\uff0c\u5e76\u4f7f\u7528table()\u51fd\u6570\u7edf\u8ba1\u6bcf\u4e2a\u80bf\u7624\u7c7b\u578b\u7684\u6837\u54c1\u6570\u76ee\u3002NormalNum\u4fdd\u5b58\u6837\u54c1\u6570\u76ee\u5927\u4e8e\u7b49\u4e8e5\u7684\u80bf\u7624\u7c7b\u578b\u3002NormalCancer\u4fdd\u5b58\u6837\u54c1\u6570\u76ee\u5927\u4e8e\u7b49\u4e8e5\u7684\u80bf\u7624\u7c7b\u578b\u7684\u540d\u79f0\u3002\u6570\u636e\u7b5b\u9009\uff1a\u4f7f\u7528which()\u51fd\u6570\u548c%in%\u64cd\u4f5c\u7b26\uff0c\u4ece\u6570\u636e\u6846\u4e2d\u9009\u62e9\u5c5e\u4e8eNormalCancer\u4e2d\u80bf\u7624\u7c7b\u578b\u7684\u6570\u636e\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7edf\u8ba1\u6bcf\u4e2a\u80bf\u7624\u6b63\u5e38\u6837\u54c1\u6570\u76ee,\u6311\u9009\u6b63\u5e38\u6837\u54c1\u6570\u76ee>=5\u7684\u80bf\u7624<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">Normal=data[data[,<span class=\"code-snippet__string\">\"Type\"<\/span>]==<span class=\"code-snippet__string\">\"Normal\"<\/span>,]<\/span><\/code><code><span class=\"code-snippet_outer\">NormalNum=table(Normal[,<span class=\"code-snippet__string\">\"CancerType\"<\/span>])<\/span><\/code><code><span class=\"code-snippet_outer\">NormalNum=NormalNum[NormalNum>=<span class=\"code-snippet__number\">5<\/span>]<\/span><\/code><code><span class=\"code-snippet_outer\">NormalCacner=<span class=\"code-snippet__built_in\">names<\/span>(NormalNum)<\/span><\/code><code><span class=\"code-snippet_outer\">data=data[which(data[,<span class=\"code-snippet__string\">\"CancerType\"<\/span>] %in% NormalCacner),]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u7bb1\u578b\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p=ggboxplot(data, x=<span class=\"code-snippet__string\">\"CancerType\"<\/span>, y=<span class=\"code-snippet__string\">\"expression\"<\/span>, color=<span class=\"code-snippet__string\">\"Type\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> xlab=<span class=\"code-snippet__string\">\"\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> ylab=paste0(gene,<span class=\"code-snippet__string\">\" expression\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> palette = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"blue\"<\/span>,<span class=\"code-snippet__string\">\"red\"<\/span>) )<\/span><\/code><code><span class=\"code-snippet_outer\">p=p+rotate_x_text(<span class=\"code-snippet__number\">60<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7ed8\u5236\u7bb1\u578b\u56fe\uff1a\u4f7f\u7528ggboxplot()\u51fd\u6570\u7ed8\u5236\u7bb1\u578b\u56fe\u3002x=\"CancerType\"\u6307\u5b9ax\u8f74\u4e3a\u764c\u75c7\u7c7b\u578b\uff0cy=\"expression\"\u6307\u5b9ay\u8f74\u4e3a\u57fa\u56e0\u8868\u8fbe\u503c\u3002color=\"Type\"\u6307\u5b9a\u6839\u636e\u6837\u54c1\u7c7b\u578b\uff08\u6b63\u5e38\/\u80bf\u7624\uff09\u8fdb\u884c\u989c\u8272\u533a\u5206\u3002xlab=\"\"\u5c06x\u8f74\u6807\u7b7e\u8bbe\u7f6e\u4e3a\u7a7a\uff0cylab=paste0(gene,\" expression\")\u8bbe\u7f6ey\u8f74\u6807\u7b7e\u4e3a\u57fa\u56e0\u540d + \"expression\"\u3002palette = c(\"blue\",\"red\")\u8bbe\u7f6e\u989c\u8272\u65b9\u6848\uff0c\u84dd\u8272\u8868\u793a\u6b63\u5e38\u6837\u54c1\uff0c\u7ea2\u8272\u8868\u793a\u80bf\u7624\u6837\u54c1\u3002rotate_x_text(60)\u5c06x\u8f74\u6807\u7b7e\u65cb\u8f6c60\u5ea6\uff0c\u4ee5\u907f\u514d\u6807\u7b7e\u91cd\u53e0\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u8f93\u51fa\u56fe\u5f62<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pdf(file=<span class=\"code-snippet__string\">\"boxplot.pdf\"<\/span>, width=<span class=\"code-snippet__number\">7.5<\/span>, height=<span class=\"code-snippet__number\">5<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">p+stat_compare_means(aes(group=Type),<\/span><\/code><code><span class=\"code-snippet_outer\"> method=<span class=\"code-snippet__string\">\"wilcox.test\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7edf\u8ba1\u5206\u6790\u65b9\u6cd5\uff08wilcox.test\uff09\u6765\u6bd4\u8f83\u4e0d\u540c\u7c7b\u578b\u4e4b\u95f4\u7684\u5dee\u5f02<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> symnum.args=<span class=\"code-snippet__built_in\">list<\/span>(cutpoints = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">0<\/span>, <span class=\"code-snippet__number\">0.001<\/span>, <span class=\"code-snippet__number\">0.01<\/span>, <span class=\"code-snippet__number\">0.05<\/span>, <span class=\"code-snippet__number\">1<\/span>), symbols = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__string\">\"***\"<\/span>, <span class=\"code-snippet__string\">\"**\"<\/span>, <span class=\"code-snippet__string\">\"*\"<\/span>, <span class=\"code-snippet__string\">\" \"<\/span>)), <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u56fe\u4e2d\u6807\u6ce8\u663e\u8457\u6027\u6c34\u5e73<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> label = <span class=\"code-snippet__string\">\"p.signif\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">###\u8bbe\u7f6e\u8f93\u51fa\u56fe\u5f62\u7684\u5927\u5c0f\u3002stat_compare_means()\u51fd\u6570\u4e8e\u5728\u7bb1\u578b\u56fe\u4e0a\u8fdb\u884c\u7edf\u8ba1\u5206\u6790\uff0c\u6807\u6ce8\u4e0d\u540c\u764c\u75c7\u7c7b\u578b\u4e4b\u95f4\u7684\u663e\u8457\u6027\u5dee\u5f02\u3002aes(group=Type)\u6307\u5b9a\u8fdb\u884c\u6bd4\u8f83\u7684\u5206\u7ec4\u53d8\u91cf\u4e3a\u6837\u54c1\u7c7b\u578b\uff08\u6b63\u5e38\/\u80bf\u7624\uff09\u3002method=\"wilcox.test\"\u4f7f\u7528Wilcoxon\u79e9\u548c\u68c0\u9a8c\u8fdb\u884c\u6bd4\u8f83\u3002symnum.args\u53c2\u6570\u5b9a\u4e49\u4e86\u663e\u8457\u6027\u6c34\u5e73\u7684\u7b26\u53f7\u8868\u793a\u3002label=\"p.signif\"\u6807\u6ce8\u663e\u8457\u6027\u6c34\u5e73\u3002dev.off()\u5173\u95edpdf\u8f93\u51fa\u3002<\/span><\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br 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