{"id":59518,"date":"2024-06-17T08:26:31","date_gmt":"2024-06-17T00:26:31","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=59518"},"modified":"2024-06-17T08:26:31","modified_gmt":"2024-06-17T00:26:31","slug":"%e7%ba%af%e4%bb%a3%e7%a0%81%e5%ae%9e%e6%93%8d%ef%bc%8c%e5%b0%8f%e6%9e%9c%e5%b8%a6%e4%bd%a0%e8%ae%a1%e7%ae%97%e5%9f%ba%e5%9b%a0%e4%b8%8e%e5%85%8d%e7%96%ab%e7%bb%86%e8%83%9e%e5%9c%a8tmb%e4%b8%ad-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=59518","title":{"rendered":"\u7eaf\u4ee3\u7801\u5b9e\u64cd\uff0c\u5c0f\u679c\u5e26\u4f60\u8ba1\u7b97\u57fa\u56e0\u4e0e\u514d\u75ab\u7ec6\u80de\u5728TMB\u4e2d\u7684\u76f8\u5173\u6027"},"content":{"rendered":"


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powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section powered-by=\"xiumi.us\">\n<section>\n<p><span style=\"font-size: 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16px;\"><strong>CIBERSORT<\/strong>\u662f\u4e00\u79cd\u5e38\u7528\u4e8e\u89e3\u6790\u80bf\u7624\u7ec4\u7ec7\u4e2d\u4e0d\u540c\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u5206\u5e03\u7684\u7b97\u6cd5\u3002\u901a\u8fc7\u5c06CIBERSORT\u7ed3\u679c\u4e0e\u7279\u5b9a\u57fa\u56e0\u7684\u8868\u8fbe\u6570\u636e\u8fdb\u884c\u76f8\u5173\u6027\u5206\u6790\uff0c\u53ef\u4ee5\u63ed\u793a\u8fd9\u4e9b\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u5728\u57fa\u56e0\u8c03\u63a7\u8fc7\u7a0b\u4e2d\u7684\u6f5c\u5728\u91cd\u8981\u6027\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\"><strong>\u672c\u671f\u4e3b\u8981\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u5355\u57fa\u56e0\u4e0e\u591a\u79cd\u514d\u75ab\u7ec6\u80de\u7684\u76f8\u5173\u6027\u5206\u6790\u7684\u5b9e\u64cd\u4fdd\u59c6\u7ea7\u4ee3\u7801\u3002<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 16px;\"><strong>\u4ee3\u7801\u4e2d\u5bf9<\/strong><strong>\u793a\u4f8b\u57fa\u56e0\u7684<\/strong>CXCR4<strong>\u7684\u8868\u8fbe\u91cf\u8fdb\u884c<\/strong><strong>\u514d\u75ab\u76f8\u5173\u5206\u6790<\/strong><strong>\u3002<\/strong><strong>\u4e0b\u9762\u662f\u4e0b\u8f7d\u6574\u7406\u7684\u6837\u672c\u8868\u8fbe\u91cf\u4fe1\u606f\u548c\u6837\u672c\u514d\u75ab\u7ec6\u80de\u4fe1\u606f\u3002<\/strong><\/span><strong><span style=\"font-family: \u5b8b\u4f53;font-size: 12pt;\"><o:p><\/o:p><\/span><\/strong><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:"Times New Roman";mso-fareast-font-family:\u5b8b\u4f53;color:rgb(36,41,47);font-size:12.0000pt;mso-font-kerning:0.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6980830670926518\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_HEWgdU3bcJWavv6ClOIGqLRdLfo7LQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxkQdNGeWtDGfzAUzolwgtQ5eobyAOE5pa1CekibT3INPDwlHEWgdU3bcJWavv6ClOIGqLRdLfo7LQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"626\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6998394863563403\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_fcpLMicDgonT3iacnl3Sy5ibkYawWA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxkQdNGeWtDGfzAUzolwgtQ34DMnY7l0we8qiakA4SyhJMwpA4hfcpLMicDgonT3iacnl3Sy5ibkYawWA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"623\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:Cambria;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:"Times New Roman";font-size:12.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<h1 style=\"text-align: center;\"><em style=\"letter-spacing: 0.034em;\"><\/em><\/h1>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">#if (!requireNamespace(\"BiocManager\", quietly = TRUE))<\/span><\/code><code><span class=\"code-snippet_outer\"># install.packages(\"BiocManager\")<\/span><\/code><code><span class=\"code-snippet_outer\">#BiocManager::install(\"limma\")<\/span><\/code><code><span class=\"code-snippet_outer\">#\u68c0\u67e5\u662f\u5426\u5b89\u88c5\u4e86\u201cBiocManager\u201d\u5305\u3002\u5982\u679c\u6ca1\u6709\u5b89\u88c5\uff0c\u5b83\u4f1a\u8fdb\u884c\u5b89\u88c5\u3002BiocManager\u7528\u4e8e\u5728R\u4e2d\u7ba1\u7406Bioconductor\u5305\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">#install.packages(\"ggplot2\")<\/span><\/code><code><span class=\"code-snippet_outer\">#install.packages(\"ggpubr\")<\/span><\/code><code><span class=\"code-snippet_outer\">#install.packages(\"ggExtra\")<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">library(limma)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggplot2)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggExtra)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5b89\u88c5\u548c\u52a0\u8f7d\u6240\u9700\u7684R\u5305\uff0c\u4f8b\u5982\u201climma\u201d\u3001\u201cggplot2\u201d\u3001\u201cggpubr\u201d\u548c\u201cggExtra\u201d\u3002\u8fd9\u4e9b\u5305\u7528\u4e8e\u5404\u79cd\u6570\u636e\u5206\u6790\u548c\u53ef\u89c6\u5316\u4efb\u52a1\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">immuneFile=\"CIBERSORT-Results.txt\" #\u514d\u75ab\u7ed3\u679c\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">expFile=\"symbol.txt\" #\u8868\u8fbe\u8f93\u5165\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">gene=\"CXCR4\" #\u57fa\u56e0\u540d\u79f0<\/span><\/code><code><span class=\"code-snippet_outer\">pFilter=0.05 #CIBERSORT\u7ed3\u679c\u8fc7\u6ee4\u6761\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5982\u201cimmuneFile\u201d\uff08\u5305\u542bCIBERSORT\u7ed3\u679c\u7684\u6587\u4ef6\uff09\u3001<\/span><\/code><code><span class=\"code-snippet_outer\">#\u201cexpFile\u201d\uff08\u8868\u8fbe\u5f0f\u8f93\u5165\u6587\u4ef6\uff09\u3001\u201cgene\u201d\uff08\u611f\u5174\u8da3\u7684\u7279\u5b9a\u57fa\u56e0\uff09\u548c\u201cpFilter\u201d\uff08CIBERSORT\u7ed3\u679c\u8fc7\u6ee4\u9608\u503c\uff09\u3002<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u8bfb\u53d6\u514d\u75ab\u7ed3\u679c\u6587\u4ef6\uff0c\u5e76\u5bf9\u6570\u636e\u8fdb\u884c\u6574\u7406<\/span><\/code><code><span class=\"code-snippet_outer\">immune=read.table(immuneFile,sep=\"t\",header=T,row.names=1,check.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\">immune=immune[immune[,\"P-value\"]<pFilter,]<\/span><\/code><code><span class=\"code-snippet_outer\">immune=as.matrix(immune[,1:(ncol(immune)-3)])<\/span><\/code><code><span class=\"code-snippet_outer\">#\u4ece\u201cimmuneFile\u201d\u4e2d\u8bfb\u53d6CIBERSORT\u7ed3\u679c\uff0c<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5e76\u8fc7\u6ee4\u6389p\u503c\u5c0f\u4e8e\u6307\u5b9a\u9608\u503c\uff08\u201cpFilter\u201d\uff09\u7684\u884c\u3002\u8fd8\u4ece\u201cimmune\u201d\u6570\u636e\u6846\u4e2d\u5220\u9664\u4e0d\u5fc5\u8981\u7684\u5217\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">#\u8bfb\u53d6\u8868\u8fbe\u6587\u4ef6\uff0c\u5e76\u5bf9\u8f93\u5165\u6587\u4ef6\u6574\u7406<\/span><\/code><code><span class=\"code-snippet_outer\">rt=read.table(expFile,sep=\"t\",header=T,check.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\">rt=as.matrix(rt)<\/span><\/code><code><span class=\"code-snippet_outer\">rownames(rt)=rt[,1]<\/span><\/code><code><span class=\"code-snippet_outer\">exp=rt[,2:ncol(rt)]<\/span><\/code><code><span class=\"code-snippet_outer\">dimnames=list(rownames(exp),colnames(exp))<\/span><\/code><code><span class=\"code-snippet_outer\">data=matrix(as.numeric(as.matrix(exp)),nrow=nrow(exp),dimnames=dimnames)<\/span><\/code><code><span class=\"code-snippet_outer\">data=avereps(data)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u4ece\u201cexpFile\u201d\u4e2d\u8bfb\u53d6\u57fa\u56e0\u8868\u8fbe\u6570\u636e\uff0c\u63d0\u53d6\u6307\u5b9a\u201cgene\u201d\u7684\u76f8\u5173\u6570\u636e\uff0c<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5e76\u5904\u7406\u6570\u636e\u4ee5\u53bb\u9664\u6b63\u5e38\u6837\u672c\u5e76\u9009\u62e9\u4e0e\u514d\u75ab\u6570\u636e\u4ea4\u96c6\u7684\u6837\u672c\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">#\u7136\u540e\uff0c\u9009\u62e9\u9664\u7b2c\u4e00\u5217\u5916\u7684\u6240\u6709\u5217\u4f5c\u4e3a\u8868\u8fbe\u6570\u636e\uff0c\u5e76\u91cd\u65b0\u5b9a\u4e49\u7ef4\u5ea6\u540d\u79f0\u3002\u6700\u540e\uff0c\u6570\u636e\u8fdb\u884c\u4e86\u5e73\u5747\u5904\u7406\u3002<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(colnames(data),\"\\-\"),\"[\",4)<\/span><\/code><code><span class=\"code-snippet_outer\">group=sapply(strsplit(group,\"\"),\"[\",1)<\/span><\/code><code><span class=\"code-snippet_outer\">group=gsub(\"2\",\"1\",group)<\/span><\/code><code><span class=\"code-snippet_outer\">data=data[,group==0]<\/span><\/code><code><span class=\"code-snippet_outer\">#\u53bb\u9664\u6b63\u5e38\u6837\u54c1\uff0c\u6839\u636e\u6837\u672c\u540d\u4e2d\u7684\u4fe1\u606f\u5224\u65ad\u662f\u5426\u4e3a\u6b63\u5e38\u6837\u672c\uff0c\u5e76\u53bb\u9664\u4e86\u6b63\u5e38\u6837\u672c\uff0c\u4fdd\u7559\u4ec5\u5305\u542b\u80bf\u7624\u6837\u672c\u7684\u6570\u636e\u3002<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">#\u6837\u54c1\u53d6\u4ea4\u96c6\u5c06\u57fa\u56e0\u7684\u8868\u8fbe\u503c\u6dfb\u52a0\u5230\u514d\u75ab\u6570\u636e\u4e2d\uff0c\u7136\u540e\u53d6\u514d\u75ab\u6570\u636e\u548c\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u7684\u4ea4\u96c6\uff0c<\/span><\/code><code><span class=\"code-snippet_outer\">#\u4ee5\u786e\u4fdd\u4e24\u8005\u5177\u6709\u76f8\u540c\u7684\u6837\u672c\u3002\u8fd9\u6837\u53ef\u4ee5\u4fdd\u8bc1\u514d\u75ab\u6570\u636e\u548c\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u4e4b\u95f4\u6709\u76f8\u540c\u7684\u6837\u672c\uff0c\u4fbf\u4e8e\u540e\u7eed\u76f8\u5173\u6027\u5206\u6790\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">data=rbind(data,gene=data[gene,])<\/span><\/code><code><span class=\"code-snippet_outer\">exp=t(data[c(\"gene\",gene),])<\/span><\/code><code><span class=\"code-snippet_outer\">sameSample=intersect(row.names(immune),row.names(exp))<\/span><\/code><code><span class=\"code-snippet_outer\">immune1=immune[sameSample,]<\/span><\/code><code><span class=\"code-snippet_outer\">exp1=exp[sameSample,]<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">outTab=data.frame()<\/span><\/code><code><span class=\"code-snippet_outer\">x=as.numeric(exp1[,1])<\/span><\/code><code><span class=\"code-snippet_outer\">#\u6309\u514d\u75ab\u7ec6\u80de\u5faa\u73af<\/span><\/code><code><span class=\"code-snippet_outer\">for(j in colnames(immune1)[1:22]){<\/span><\/code><code><span class=\"code-snippet_outer\"> y=as.numeric(immune1[,j])<\/span><\/code><code><span class=\"code-snippet_outer\"> if(sd(y)>0.001){<\/span><\/code><code><span class=\"code-snippet_outer\"> df1=as.data.frame(cbind(x,y))<\/span><\/code><code><span class=\"code-snippet_outer\"> corT=cor.test(x,y,method=\"spearman\")<\/span><\/code><code><span class=\"code-snippet_outer\"> cor=corT$estimate<\/span><\/code><code><span class=\"code-snippet_outer\"> pValue=corT$p.value<\/span><\/code><code><span class=\"code-snippet_outer\"> p1=ggplot(df1, aes(x, y)) + <\/span><\/code><code><span class=\"code-snippet_outer\"> ylab(j)+xlab(gene)+<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point()+ geom_smooth(method=\"lm\",formula=y~x) + theme_bw()+<\/span><\/code><code><span class=\"code-snippet_outer\"> stat_cor(method = 'spearman', aes(x =x, y =y))<\/span><\/code><code><span class=\"code-snippet_outer\"> if(pValue<pFilter){<\/span><\/code><code><span class=\"code-snippet_outer\"> pdf(file=paste0(j,\".pdf\"),width=5,height=5)<\/span><\/code><code><span class=\"code-snippet_outer\"> print(p1)<\/span><\/code><code><span class=\"code-snippet_outer\"> dev.off()<\/span><\/code><code><span class=\"code-snippet_outer\"> outTab=rbind(outTab,cbind(Cell=j,pValue))<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\">#\u76f8\u5173\u6027\u68c0\u9a8c\u8fdb\u884c\u76f8\u5173\u6027\u68c0\u9a8c\uff0c\u8ba1\u7b97\u57fa\u56e0\u8868\u8fbe\u503c\uff08x\uff09\u4e0e\u5404\u79cd\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u6bd4\u4f8b\uff08y\uff09\u4e4b\u95f4\u7684Spearman\u79e9\u76f8\u5173\u7cfb\u6570\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5faa\u73af\u904d\u5386\u6bcf\u79cd\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\uff0c\u5e76\u7ed8\u5236\u6563\u70b9\u56fe\u548c\u56de\u5f52\u7ebf\uff0c\u4ee5\u53ca\u6807\u6ce8\u76f8\u5173\u7cfb\u6570\u548cp\u503c\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5982\u679c\u76f8\u5173\u6027\u663e\u8457\uff08pValue<pFilter\uff09\uff0c\u5219\u5c06\u6563\u70b9\u56fe\u4fdd\u5b58\u4e3a\u4ee5\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u547d\u540d\u7684PDF\u6587\u4ef6\uff0c<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5e76\u5c06\u7ed3\u679c\u5b58\u50a8\u5728\u6570\u636e\u6846\"outTab\"\u4e2d\u3002\u6700\u540e\uff0c\u5c06\u5305\u542b\u663e\u8457\u76f8\u5173\u6027\u7684\u7ed3\u679c\u5b58\u50a8\u5728\u540d\u4e3a\"immuneCor.result.txt\"\u7684\u6587\u672c\u6587\u4ef6\u4e2d<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(outTab,file=\"immuneCor.result.txt\",sep=\"t\",row.names=F,quote=F)<\/span><\/code><\/pre>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4308617234468938\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_o9RpaMYHNlS11627TNR7INeyX69eyQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxkQdNGeWtDGfzAUzolwgtQpNEhcfyxMjHMMPuJ7SQiaicEEwo9RpaMYHNlS11627TNR7INeyX69eyQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"499\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.00199203187251\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_rcZttesic92jPibc0GbnCdFoSgI7XQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxkQdNGeWtDGfzAUzolwgtQE2ibCJ3G5LpDsv80v1Lps7CjibtsrcZttesic92jPibc0GbnCdFoSgI7XQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"502\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9917864476386037\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_NtbLSR47riaibckL3Rxq23LQDkSdjg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxkQdNGeWtDGfzAUzolwgtQz6JEjxPqRI5DwYQ3QLicMqJcKXGNtbLSR47riaibckL3Rxq23LQDkSdjg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"487\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9879032258064516\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_ZPv0eAZtXaLlesicwN0cIEWmp8V4DA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxkQdNGeWtDGfzAUzolwgtQ8q1jdKqLeeChGtaIPKY4fegCmZPv0eAZtXaLlesicwN0cIEWmp8V4DA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"496\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9637096774193549\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_FdctS7aE3ScrLtRHsfLffiaTg2ZEzA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxkQdNGeWtDGfzAUzolwgtQsX6dNVLaCjUyJpFjoLO7hSekcFdctS7aE3ScrLtRHsfLffiaTg2ZEzA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"496\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 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