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class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-09-04 19:02:21<\/em><\/div>\n<p><content><\/p>\n<section style=\"font-size: 16px;\" data-mpa-powered-by=\"yiban.io\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: left;\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section style=\"letter-spacing: 0.578px;\">\n<section>\n<section style=\"width: 100%;flex: 0 0 100%;\" powered-by=\"xiumi.us\">\n<section style=\"opacity: 1;width: 100%;\">\n<section powered-by=\"xiumi.us\">\n<section style=\"line-height: 1.8;\">\n<section style=\"margin-top: 10px;margin-bottom: 20px;width: 100%;\" powered-by=\"xiumi.us\">\n<section style=\"flex-flow: row nowrap;text-align: center;width: 100%;\">\n<section style=\"display: flex;justify-content: center;flex-direction: row;\">\n<section style=\"display: inline-block;width: 100%;flex: 0 0 auto;align-self: flex-start;vertical-align: top;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;border-style: solid;border-width: 1px;border-color: rgb(67, 67, 67);height: auto;box-shadow: rgb(255, 219, 118) 6px 6px 0px;background-color: rgb(255, 255, 255);width: 100%;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row nowrap;\">\n<section style=\"display: inline-block;vertical-align: top;width: 70.0348%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 70.0348% !important;\">\n<section style=\"margin-top: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u751f\u4fe1\u4ebaR\u8bed\u8a00\u5b66\u4e60\u5fc5\u5907<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u7acb\u523b\u62e5\u6709\u4e00\u4e2aRstudio\u8d26\u53f7<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\"><strong><span style=\"font-size: 15px;\">\u5f00\u542f\u5347\u7ea7\u6a21\u5f0f\u5427<\/span><\/strong><\/span><\/p>\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"color: rgb(255, 129, 36);font-size: 12px;letter-spacing: 0.5px;\">\uff0856\u7ebf\u7a0b\uff0c256G\u5185\u5b58\uff0c\u4e2a\u4eba\u5b58\u50a81T\uff09<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 29.9652%;align-self: flex-start;flex: 0 0 auto;height: auto;max-width: 29.9652% !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;\" powered-by=\"xiumi.us\">\n<section style=\"text-align: center;line-height: 0;\">\n<section>\n<section style=\"display: inline-block;vertical-align: middle;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 0;\">\n<section style=\"width: 111px;\" powered-by=\"xiumi.us\">\n<section style=\"line-height: 1.75em;text-indent: 0em;\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;height: auto;width: 111px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_GBhsibRAgNiaQsH4ichdP9ibEhHMFA-123.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyL6u7wJswOp8vtpzGp6vYngnlT6jiavu08Bvp2iblzGf3IkoBD9NGBhsibRAgNiaQsH4ichdP9ibEhHMFA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"400\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<section style=\"display: contents;\" powered-by=\"xiumi.us\">\n<section style=\"display: contents;\">\n<section style=\"display: contents;\">\n<section style=\"height: 0px;overflow: hidden;\"><span style=\"font-size: 14px;letter-spacing: 0.578px;caret-color: red;\"> <\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"white-space: normal;\"><span style=\"font-size: 14px;\"> <\/span><\/p>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0d\u77e5\u9053\u6709\u591a\u5c11\u521a\u5f00\u59cb\u63a5\u89e6\u5355\u7ec6\u80de\u6d4b\u5e8f\u5206\u6790\u7684\u5c0f\u4f19\u4f34\u5728\u9762\u5bf9\u65e0\u7a77\u65e0\u5c3d\u7684R\u5305\u548c\u4ee3\u7801\u65f6\u611f\u5230\u754f\u96be\uff0c\u4eff\u4f5b\u4e00\u5806\u77e5\u8bc6\u8981\u5f3a\u884c\u6d8c\u5165\u6211\u4eec\u7684\u8111\u6d77\u3002\u4eca\u5929\u5c0f\u679c\u60f3\u5411\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e2aR\u5305\u2014\u2014popsicleR\u3002\u8be5R\u5305\u7684\u4f5c\u8005\u6574\u5408\u4e86\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5206\u6790\u6240\u9700\u7684R\u5305\uff0c\u7528\u4e03\u884c\u4ee3\u7801\u5c31\u53ef\u4ee5\u5b8c\u6210\u4ece\u8d28\u63a7\u5230\u7ec6\u80de\u6ce8\u91ca\u7684\u6574\u5957\u6d41\u7a0b\u3002\u5b66\u4e60\u4f7f\u7528\u8be5R\u5305\u53ef\u4ee5\u4e3a\u521d\u5b66\u8005\u6784\u5efa\u601d\u7ef4\u6846\u67b6\uff0c\u4ece\u800c\u4f7f\u5b66\u4e60\u8fc7\u7a0b\u66f4\u52a0\u7b80\u5355\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0b\u9762\uff0c\u5c0f\u679c\u5c06\u4e3a\u5927\u5bb6\u8be6\u7ec6\u4ecb\u7ecd\u4e00\u4e0bpopsicle\u3002\u5b83\u7ed3\u5408\u4e86\u5e7f\u6cdb\u4f7f\u7528\u7684\u6d41\u7a0b\u4e2d\u5b9e\u73b0\u7684\u65b9\u6cd5\uff0c\u4ee5\u4ea4\u4e92\u65b9\u5f0f\u6267\u884c scRNA-seq \u6570\u636e\u5206\u6790\u7684\u6240\u6709\u4e3b\u8981\u9884\u5904\u7406\u548c QC \u6b65\u9aa4\u3002\u8be5\u8f6f\u4ef6\u5305\u7531\u4e03\u4e2a\u529f\u80fd\u7ec4\u4ef6\u7ec4\u6210\uff1a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 1\uff1a\u6267\u884c\u8d28\u91cf\u63a7\u5236\u6307\u6807\u7684\u63a2\u7d22<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 2\uff1a\u7ec6\u80de\u7b5b\u9009<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 3\uff1a\u53cc\u7ec6\u80de\u68c0\u6d4b<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 4\uff1a\u6570\u636e\u6807\u51c6\u5316<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 5\uff1a\u7f29\u653e\u53ca\u56de\u5f52<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 6\uff1a\u7ec6\u80de\u805a\u7c7b<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 7\uff1a\u7ec6\u80de\u6ce8\u91ca<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.8372093023255813\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_wZxvJu7w8OqDnW4AJp7IwSiczaNNzQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEDfselIjP3okKave9Wxz5lznVRwZxvJu7w8OqDnW4AJp7IwSiczaNNzQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"301\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><strong><span style=\"font-family: \u7b49\u7ebf;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/strong><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u5206\u6790\u7684\u6bcf\u4e2a\u6b65\u9aa4\u4e2d\uff0cpopsicleR \u901a\u8fc7\u5f69\u8272\u6587\u672c\u6d88\u606f\u4ea4\u4e92\u5f0f\u5730\u6307\u5bfc\u7528\u6237\uff0c\u5e76\u5728\u4e13\u7528\u6587\u4ef6\u5939\u4e2d\u4fdd\u5b58\u5404\u79cd\u56fe\uff0c\u4ee5\u7814\u7a76\u591a\u4e2a QC \u6307\u6807\u5e76\u8bc4\u4f30\u8fc7\u6ee4\u548c\u56de\u5f52\u53c2\u6570\u5bf9\u7ec6\u80de\u7fa4\u8bc6\u522b\u548c\u5206\u7c7b\u7684\u5f71\u54cd\u3002\u8dd1\u5b8c\u6d41\u7a0b\u540e\u53ef\u4e3a\u5c0f\u4f19\u4f34\u4eec\u4ea7\u51fa20\u591a\u5f20\u5404\u5f0f\u5404\u6837\u4e14\u7f8e\u89c2\u7684\u56fe\u8868\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5c0f\u679c\u8fd9\u8fb9\u5df2\u7ecf\u4e3a\u5c0f\u4f19\u4f34\u4eec\u51c6\u5907\u597d\u8f93\u5165\u6587\u4ef6\u4e86\uff0c\u83b7\u53d6\u94fe\u63a5\u653e\u5728\u6587\u7ae0\u7684\u6700\u540e\u54e6~\u5c31\u8ba9\u6211\u4eec\u6b63\u5f0f\u5f00\u59cb\u4eca\u5929\u7684\u5b66\u4e60\u5427\uff01<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"># Step 0 \u5b89\u88c5popsicleR\u5305\u53ca\u5176\u4f9d\u8d56<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u56e0\u4e3apopsicleR\u6574\u5408\u5927\u91cf\u7684R\u5305\uff0c\u56e0\u6b64\u5728\u5b89\u88c5\u4f9d\u8d56\u7684\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u6709\u4e00\u4e9b\u7e41\u7410\u54e6\uff0c\u4e0b\u9762\u7684\u4ee3\u7801\u867d\u7136\u5f88\u591a\uff0c\u4f46\u53ea\u9700\u8981\u5c0f\u4f19\u4f34\u4eec\u76f4\u63a5\u8fd0\u884c\u5c31\u53ef\u4ee5\u5566\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">options(timeout = 999) # \u65b9\u4fbf\u5728GitHub\u4e0a\u4e0b\u8f7d\u5bf9\u5e94\u7684R\u5305<\/span><span style=\"\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"\"><br \/><\/span><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\">CRANdep <- c(\"Seurat\",\"reticulate\",\"R.utils\",\"dplyr\",\"ggplot2\",\"clustree\",\"ape\",\"gtools\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"future\",\"grid\",\"gridExtra\",\"magrittr\",\"limma\",\"patchwork\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"crayon\",\"ggExtra\",\"RColorBrewer\",\"ggplotify\",\"RANN\",\"umap\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"celldex\",\"curl\",\"httr\",\"lattice\",\"shinythemes\",\"usethis\",\"rcmdcheck\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"roxygen2\",\"rversions\",\"devtools\",\"pheatmap\",\"BiocManager\",\"corrplot\")<\/span><\/code><code><span class=\"code-snippet_outer\">newPackages <- CRANdep[!(CRANdep %in% installed.packages()[,\"Package\"])]<\/span><\/code><code><span class=\"code-snippet_outer\">if(length(newPackages)){install.packages(newPackages)}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">CRANarcdep <- c(\"Matrix\",\"optimbase\",\"optimsimplex\",\"neldermead\",\"session\")<\/span><\/code><code><span class=\"code-snippet_outer\">newPackages <- CRANarcdep[!(CRANarcdep %in% installed.packages()[,\"Package\"])]<\/span><\/code><code><span class=\"code-snippet_outer\">if(length(newPackages)){<\/span><\/code><code><span class=\"code-snippet_outer\"> packagesurl <- c(\"https:\/\/cran.r-project.org\/src\/contrib\/Archive\/Matrix\/Matrix_1.3-2.tar.gz\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"https:\/\/cran.r-project.org\/src\/contrib\/Archive\/optimbase\/optimbase_1.0-9.tar.gz\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"https:\/\/cran.r-project.org\/src\/contrib\/Archive\/optimsimplex\/optimsimplex_1.0-7.tar.gz\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"https:\/\/cran.r-project.org\/src\/contrib\/Archive\/neldermead\/neldermead_1.0-11.tar.gz\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"https:\/\/cran.r-project.org\/src\/contrib\/Archive\/session\/session_1.0.3.tar.gz\")<\/span><\/code><code><span class=\"code-snippet_outer\"> for (i in 1:length(newPackages)){<\/span><\/code><code><span class=\"code-snippet_outer\"> source_repo <- packagesurl[grep(newPackages[i], packagesurl)]<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(source_repo, repos=NULL, type=\"source\")<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">BioCdep <- c(\"SingleR\",\"limma\",\"BiocFileCache\",\"AnnotationHub\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"ExperimentHub\",\"celldex\",\"scDblFinder\")<\/span><\/code><code><span class=\"code-snippet_outer\">newPackages <- BioCdep[!(BioCdep %in% installed.packages()[,\"Package\"])]<\/span><\/code><code><span class=\"code-snippet_outer\">if(length(newPackages)){BiocManager::install(newPackages)}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">if(!\"scMCA\"%in% installed.packages()[,\"Package\"]){devtools::install_github(\"ggjlab\/scMCA\")}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">devtools::install_github(\"bicciatolab\/popsicleR\")<\/span><\/code><code><span class=\"code-snippet_outer\">library(popsicleR)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u8f7d\u5165R\u5305\u540e\u51fa\u73b0<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.14285714285714285\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_kDeGxAgKvbs3CKhthCbpHDNvl4fEKQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEBRj58q3Huvx1wlicz9W0iasTiaokDeGxAgKvbs3CKhthCbpHDNvl4fEKQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5373\u4e3a\u5b89\u88c5\u6210\u529f\uff0c\u6211\u4eec\u4fbf\u53ef\u4ee5\u8fdb\u884c\u4e0b\u4e00\u6b65\u5566<\/span><span style=\"\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\"># step 1 \u4ece\u539f\u59cb\u6570\u636e\u521b\u5efa Seurat \u5bf9\u8c61\u5e76\u53ef\u89c6\u5316 QC \u6307\u6807<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- PrePlots(sample = 'CRR073027',<\/span><\/code><code><span class=\"code-snippet_outer\"> input_data = 'filtered_feature_bc_matrix\/',<\/span><\/code><code><span class=\"code-snippet_outer\"> percentage = 0.1, gene_filter = 200, cellranger = TRUE, organism = \"human\"<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"mso-spacerun:"yes";font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:"Times New Roman";\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@para sample: \u7814\u7a76\u9879\u76ee\u7684name\uff1b<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@para input_data: \u8f93\u5165\u6587\u4ef6\u7684\u8def\u5f84\uff0c\u5728\u8fd9\u9762\u5c0f\u4f19\u4f34\u4eec\u65e2\u53ef\u4ee5\u4f7f\u7528\u5c0f\u679c\u4e3a\u5927\u5bb6\u51c6\u5907\u597d\u7684cellranger\u7684\u77e9\u9635\u7ed3\u679c\uff0c\u4e5f\u53ef\u4ee5\u662f\u975ecellranger\u7684\u666e\u901a\u8ba1\u6570\u77e9\u9635\uff0c\u90fd\u662f\u517c\u5bb9\u7684\u54e6<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@para genelist: \u5728\u8fd9\u91cc\u5982\u679c\u5c0f\u4f19\u4f34\u4eec\u6709\u5c5e\u4e8e\u81ea\u5df1\u7684\u4e00\u5957\u4e0e\u7ec6\u80de\u5bf9\u5e94\u7684marker\u5217\u8868\u4e5f\u53ef\u4ee5\u4f7f\u7528\u5411\u91cf\u7684\u5f62\u5f0f\u8fdb\u884c\u6dfb\u52a0\u54e6\uff0c\u5c0f\u767d\u6ca1\u6709\u7684\u8bdd\uff0c\u4e5f\u6ca1\u6709\u5173\u7cfb\uff0c\u4f5c\u8005\u4e3a\u5927\u5bb6\u51c6\u5907\u597d\u4e86\u4e00\u5957marker\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@para percentage: \u8fd9\u91cc\u9650\u5236\u4e86\u6240\u6709\u7684\u57fa\u56e0\u8d77\u7801\u57281%\u7684\u7ec6\u80de\u4e2d\u8fdb\u884c\u8868\u8fbe\uff0c\u9ed8\u8ba4\u503c\u4e3a0.1<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@para gene_filter: \u9650\u5236\u6240\u6709\u7684\u7ec6\u80de\u6700\u8d77\u7801\u68c0\u6d4b\u51fa200\u4e2a\u57fa\u56e0<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@cellranger: \u82e5\u8f93\u5165\u6587\u4ef6\u4e3acellranger\u7684\u8f93\u51fa\u6587\u4ef6\u5219\u8bbe\u5b9a\u4e3aTRUE<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@organism: \u9009\u62e9\u7814\u7a76\u7684\u7269\u79cd<\/span><span style=\"\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0b\u9762\u5c0f\u679c\u4e3a\u5927\u5bb6\u7b5b\u9009\u4e86\u8fd0\u884c\u5b8c\u8d28\u63a7\u7ed3\u679c\u540e\u7684\u6570\u5f20\u7ed3\u679c\u56fe\u7ed9\u5404\u4f4d\u5c0f\u4f19\u4f34\u9884\u89c8\u4e00\u4e0b<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.28715728715728717\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_zyz4UlpTHoyY1DqiaLJLkcqVcV5wbw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEhJTJN20vzkZmU1OSOaMoTDMAkzyz4UlpTHoyY1DqiaLJLkcqVcV5wbw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6585365853658537\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_D6Ac83flSQyy2rwzPzlcTJ6sZaru3g.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkE17abgasoDXzagPaFwAJiafW8QD6Ac83flSQyy2rwzPzlcTJ6sZaru3g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"574\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.0053191489361701\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_pcxwGbeQurCnRYcPj5TCIyqM0iac6g.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEFeichW5XtE4yE5tBCibOZJ0XB40pcxwGbeQurCnRYcPj5TCIyqM0iac6g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"376\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_opsjKibOX3JrynicW5qbsvicFiacvA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkE9L6L0WJ6uGelrgaefjo0fgRia8cnLopsjKibOX3JrynicW5qbsvicFiacvA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"355\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"ruby\"><code><span class=\"code-snippet_outer\"># step 2 \u53cc\u91cd\u7ec6\u80de\u68c0\u6d4b<\/span><\/code><code><span class=\"code-snippet_outer\">\u8be5\u5904\u4ee3\u7801\u7684\u8fd0\u884c\u65f6\u95f4\u4f1a\u8f83\u957f\uff0c\u6709\u6761\u4ef6\u7684\u5c0f\u4f19\u4f34\u5efa\u8bae\u4f7f\u7528\u670d\u52a1\u5668\u8fdb\u884c\u8fd0\u884c\u54e6~<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- CalculateDoublets(UMI = seurat_obj, method = \"scrublet\", dbs_thr ='none', dbs_remove = FALSE)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># step 3 \u53cc\u91cd\u7ec6\u80de\u79fb\u9664<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- CalculateDoublets(UMI = seurat_obj, method = \"scrublet\", dbs_thr = 0.22, dbs_remove = TRUE)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u53c2\u6570\u8bf4\u660e:<\/span><\/code><code><span class=\"code-snippet_outer\">@para UMI: \u8f93\u5165\u7684\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u5bf9\u8c61<\/span><\/code><code><span class=\"code-snippet_outer\">@para method: \u53cc\u91cd\u7ec6\u80de\u68c0\u6d4b\u65b9\u6cd5\uff0c\u53ef\u4ee5\u9009\u62e9\"scrublet\",\"scDblFinder\"\u5176\u4e2d\u4e00\u79cd\u65b9\u5f0f<\/span><\/code><code><span class=\"code-snippet_outer\"># \u6765\u81ea\u5c0f\u679c\u7684\u6e29\u99a8\u63d0\u793a\uff0cdbs_thr\u53c2\u6570\u662f\u5728\u9009\u62e9scrublet\u65b9\u6cd5\u7684\u60c5\u51b5\u4e0b\u624d\u8fdb\u884c\u9009\u62e9\u7684\u54e6<\/span><\/code><code><span class=\"code-snippet_outer\">@para dbs_thr: \u8bbe\u5b9a\u7684\u53cc\u91cd\u7ec6\u80de\u9608\u503c\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3a'none'<\/span><\/code><code><span class=\"code-snippet_outer\">@para dbs_thr: \u8bbe\u5b9a\u7684\u53cc\u91cd\u7ec6\u80de\u9608\u503c\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3a0.22<\/span><\/code><code><span class=\"code-snippet_outer\">@para dbs_remove: \u662f\u5426\u79fb\u9664\u53cc\u91cd\u7ec6\u80de<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># step 4\u6807\u51c6\u5316<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- Normalize(UMI = seurat_obj, variable_genes = 2000)<\/span><\/code><code><span class=\"code-snippet_outer\"># \u53c2\u6570\u8bf4\u660e: <\/span><\/code><code><span class=\"code-snippet_outer\"># @para UMI: \u8f93\u5165\u7684\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u5bf9\u8c61<\/span><\/code><code><span class=\"code-snippet_outer\"># @para variable_genes: \u9009\u62e9\u7684\u53d8\u5f02\u57fa\u56e0\u6570\u91cf\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3a2000 <\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># step 5 \u56de\u5f52<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- ApplyRegression(UMI = seurat_obj, organism = \"human\", variables = \"none\", explore_PC = FALSE)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># step 6\u5bf9PC\u503c\u8fdb\u884c\u89c2\u6d4b\u540e\u8fdb\u884c\u56de\u5f52\u7814\u7a76<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- ApplyRegression(UMI = seurat_obj, organism = \"human\", variables = \"none\", explore_PC = TRUE)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u53c2\u6570\u8bf4\u660e: <\/span><\/code><code><span class=\"code-snippet_outer\">@para UMI: \u8f93\u5165\u7684\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u5bf9\u8c61<\/span><\/code><code><span class=\"code-snippet_outer\">@para organism: \u7814\u7a76\u7684\u7269\u79cd\uff0c\u8fd9\u91cc\u9009\u62e9\u4e86\"human\"<\/span><\/code><code><span class=\"code-snippet_outer\">@para variables: \u8fdb\u884c\u56de\u5f52\u5206\u6790\u7684\u53d8\u91cf\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3a\"none\"<\/span><\/code><code><span class=\"code-snippet_outer\"># \u5728\u6211\u4eec\u63a2\u7d22\u4e3b\u6210\u5206\u7684\u4e2a\u6570\u540e\u4fbf\u53ef\u5bf9\u8be5\u53c2\u6570\u8fdb\u884c\u8c03\u6574<\/span><\/code><code><span class=\"code-snippet_outer\">@para explore_PC: \u662f\u5426\u63a2\u7d22\u4e3b\u6210\u5206\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3aFALSE <\/span><\/code><code><span class=\"code-snippet_outer\">@para explore_PC: \u662f\u5426\u63a2\u7d22\u4e3b\u6210\u5206\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3aTRUE<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># step 7\u7ec6\u80de\u7c7b\u578b\u6ce8\u91ca<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- MakeAnnotation(UMI = seurat_obj, organism = \"human\", marker.list = \"none\", cluster_res= 0.8)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u53c2\u6570\u8bf4\u660e: <\/span><\/code><code><span class=\"code-snippet_outer\">@para UMI: \u8f93\u5165\u7684\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u5bf9\u8c61<\/span><\/code><code><span class=\"code-snippet_outer\">@para organism: \u7814\u7a76\u7684\u7269\u79cd\uff0c\u8fd9\u91cc\u9009\u62e9\u4e86\"human\"<\/span><\/code><code><span class=\"code-snippet_outer\">@para marker.list: \u7ec6\u80de\u7c7b\u578b\u6807\u8bb0\u57fa\u56e0\u5217\u8868\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3a\"none\"<\/span><\/code><code><span class=\"code-snippet_outer\">@para cluster_res: \u805a\u7c7b\u5206\u8fa8\u7387\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3a0.8<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u53ef\u4ee5\u770b\u51fa\uff0c\u867d\u7136\u8fd9\u6bb5\u4ee3\u7801\u67097\u884c\uff0c\u4f46\u5b9e\u9645\u4e0a\u6709\u4e24\u884c\u4ee3\u7801\u662f\u4e3a\u4e86\u91cd\u590d\u8fd0\u884c\u4ee5\u9009\u62e9\u53c2\u6570\u7684\uff0c\u56e0\u6b64\u6211\u4eec\u53ef\u4ee5\u53ea\u75285\u884c\u4ee3\u7801\u5c31\u5b8c\u6210\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5206\u6790\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">popsicleR\u8fd9\u4e2aR\u5305\u7684\u6e90\u4ee3\u7801\u5c06\u8fd1\u67092000\u884c\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u8fdb\u5165\u5b98\u65b9\u6587\u6863\u8fdb\u884c\u5b66\u4e60\u54e6\uff08https:\/\/github.com\/bicciatolab\/popsicleR\/\uff09\uff0c\u8fd9\u4e0d\u4ec5\u4ec5\u662f\u5bf9\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5206\u6790\u6d41\u7a0b\u7684\u5b66\u4e60\uff0c\u8fd8\u53ef\u4ee5\u901a\u8fc7\u9605\u8bfb\u6e90\u7801\u4e86\u89e3\u5230\u5404\u79cd\u5404\u6837\u7684\u8868\u683c\u7684\u7ed8\u5236\u4ee3\u7801\uff0c\u5728\u65e5\u540e\u7684\u7814\u7a76\u5b66\u4e60\u4e2d\u9887\u6709\u76ca\u5904\u3002<\/span><span style=\"text-align: center;letter-spacing: 0.034em;\"><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u597d\u5566~\u4eca\u5929\u5c0f\u679c\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\u5566\uff01\u5e0c\u671b\u4eca\u5929\u5c0f\u679c\u7684\u5206\u4eab\u53ef\u4ee5\u5e2e\u5230\u521a\u5f00\u59cb\u5165\u95e8\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5206\u6790\u7684\u5c0f\u4f19\u4f34\u4eec\u54e6~\u5982\u679c\u5c0f\u4f19\u4f34\u6709\u5176\u4ed6\u6570\u636e\u5206\u6790\u9700\u6c42\uff0c\u53ef\u4ee5\u5c1d\u8bd5\u4f7f\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\u4e91\u5e73\u53f0\uff0c\u96f6\u4ee3\u7801\u5b8c\u6210\u5206\u6790\uff0c\u975e\u5e38\u65b9\u4fbf\u5965\uff0c\u4e91\u5e73\u53f0\u7f51\u5740\u4e3a\uff1a(<\/span><span style=\"color: rgb(5, 99, 193);text-decoration: underline;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">http:\/\/www.biocloudservice.com\/home.html<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">)\uff0c\u5176\u4e2d\u4e5f\u5305\u62ec\u4e86\u901a\u8def\u8868\u8fbe\u5206\u6790(<\/span><span style=\"color: rgb(5, 99, 193);text-decoration: underline;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">http:\/\/www.biocloudservice.com\/313\/313.php<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">)\uff0c\u5355\u7ec6\u80de\u7684\u57fa\u56e0\u5171\u8868\u8fbe\u5206\u6790(<\/span><span style=\"color: rgb(5, 99, 193);text-decoration: underline;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">http:\/\/www.biocloudservice.com\/906\/906.php<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">)\u7b49\u5404\u79cd\u5c0f\u5de5\u5177\u54e6~\uff0c\u6709\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u767b\u5f55\u7f51\u7ad9\u8fdb\u884c\u4e86\u89e3\u3002<\/span><span style=\"\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"color: rgb(255, 0, 0);font-size: 10.5pt;background: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u4e91\u751f\u4fe1\u5e73\u53f0\u94fe\u63a5\uff1a<\/span><span style=\"text-decoration: underline;font-family: \u5fae\u8f6f\u96c5\u9ed1;color: rgb(255, 0, 0);background: rgb(255, 255, 255);\">http:\/\/www.biocloudservice.com\/home.html\u3002<\/span><span style=\"mso-spacerun:"yes";font-family:\u5fae\u8f6f\u96c5\u9ed1;color:rgb(255,0,0);font-size:10.5000pt;mso-font-kerning:1.0000pt;background:rgb(255,255,255);mso-shading:rgb(255,255,255);\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:\u5fae\u8f6f\u96c5\u9ed1;color:rgb(255,0,0);font-size:10.5000pt;mso-font-kerning:1.0000pt;background:rgb(255,255,255);mso-shading:rgb(255,255,255);\">\u4e91\u751f\u4fe1\u5e73\u53f0\u94fe\u63a5\uff1ahttp:\/\/www.biocloudservice.com\/home.html\u3002<\/span><span style=\"mso-spacerun:"yes";font-family:\u5b8b\u4f53;font-size:12.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-top: 0.5em;\">\n<section style=\"padding: 0.5em;border-width: 1px;border-style: solid;box-shadow: rgb(226, 226, 226) 0px 16px 1px -13px;border-color: rgb(0, 150, 136);\">\n<section style=\"text-align: left;\">\n<section style=\"color: rgb(255, 129, 36);text-align: justify;\">\n<section style=\"margin-bottom: 8px;\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u70b9\u51fb<strong><span style=\"color: rgb(0, 150, 136);\">\u201c\u9605\u8bfb\u539f\u6587\u201d<\/span><\/strong>\u7acb\u523b\u62e5\u6709<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<p style=\"text-indent: 0em;line-height: 1.75em;\"><span style=\"letter-spacing: 0.5px;\">\u2193\u2193\u2193 <\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247490130&idx=1&sn=1578a7fab283ba24e1ce5cd6087e2d0d&chksm=cf7c8a97f80b038111f1d3df359dbee67a49462b61b5bfed1060dd83c5d7b975f0db40f4626a#rd\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<p><\/content><\/p>\n<div class=\"msg_source_url\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247490130&idx=1&sn=1578a7fab283ba24e1ce5cd6087e2d0d&chksm=cf7c8a97f80b038111f1d3df359dbee67a49462b61b5bfed1060dd83c5d7b975f0db40f4626a#rd\" target=\"_blank\" rel=\"noopener\">\u9605\u8bfb\u539f\u6587<\/a><\/div>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8 \u539f\u521b \u5c0f\u679c \u751f […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/59623"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=59623"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/59623\/revisions"}],"predecessor-version":[{"id":59631,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/59623\/revisions\/59631"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=59623"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=59623"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=59623"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

{"id":59623,"date":"2024-06-17T08:34:16","date_gmt":"2024-06-17T00:34:16","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=59623"},"modified":"2024-06-17T08:34:16","modified_gmt":"2024-06-17T00:34:16","slug":"%e5%af%b9%e5%8d%95%e7%bb%86%e8%83%9e%e5%88%86%e6%9e%90%e6%af%ab%e6%97%a0%e5%a4%b4%e7%bb%aa%ef%bc%9f%e8%ae%a9popsicler%e9%a2%86%e4%bd%a0%e5%85%a5%e9%97%a8-2","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=59623","title":{"rendered":"\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8"},"content":{"rendered":"

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14px;\">\u4e0d\u77e5\u9053\u6709\u591a\u5c11\u521a\u5f00\u59cb\u63a5\u89e6\u5355\u7ec6\u80de\u6d4b\u5e8f\u5206\u6790\u7684\u5c0f\u4f19\u4f34\u5728\u9762\u5bf9\u65e0\u7a77\u65e0\u5c3d\u7684R\u5305\u548c\u4ee3\u7801\u65f6\u611f\u5230\u754f\u96be\uff0c\u4eff\u4f5b\u4e00\u5806\u77e5\u8bc6\u8981\u5f3a\u884c\u6d8c\u5165\u6211\u4eec\u7684\u8111\u6d77\u3002\u4eca\u5929\u5c0f\u679c\u60f3\u5411\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e2aR\u5305\u2014\u2014popsicleR\u3002\u8be5R\u5305\u7684\u4f5c\u8005\u6574\u5408\u4e86\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5206\u6790\u6240\u9700\u7684R\u5305\uff0c\u7528\u4e03\u884c\u4ee3\u7801\u5c31\u53ef\u4ee5\u5b8c\u6210\u4ece\u8d28\u63a7\u5230\u7ec6\u80de\u6ce8\u91ca\u7684\u6574\u5957\u6d41\u7a0b\u3002\u5b66\u4e60\u4f7f\u7528\u8be5R\u5305\u53ef\u4ee5\u4e3a\u521d\u5b66\u8005\u6784\u5efa\u601d\u7ef4\u6846\u67b6\uff0c\u4ece\u800c\u4f7f\u5b66\u4e60\u8fc7\u7a0b\u66f4\u52a0\u7b80\u5355\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0b\u9762\uff0c\u5c0f\u679c\u5c06\u4e3a\u5927\u5bb6\u8be6\u7ec6\u4ecb\u7ecd\u4e00\u4e0bpopsicle\u3002\u5b83\u7ed3\u5408\u4e86\u5e7f\u6cdb\u4f7f\u7528\u7684\u6d41\u7a0b\u4e2d\u5b9e\u73b0\u7684\u65b9\u6cd5\uff0c\u4ee5\u4ea4\u4e92\u65b9\u5f0f\u6267\u884c scRNA-seq \u6570\u636e\u5206\u6790\u7684\u6240\u6709\u4e3b\u8981\u9884\u5904\u7406\u548c QC \u6b65\u9aa4\u3002\u8be5\u8f6f\u4ef6\u5305\u7531\u4e03\u4e2a\u529f\u80fd\u7ec4\u4ef6\u7ec4\u6210\uff1a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 1\uff1a\u6267\u884c\u8d28\u91cf\u63a7\u5236\u6307\u6807\u7684\u63a2\u7d22<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 2\uff1a\u7ec6\u80de\u7b5b\u9009<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 3\uff1a\u53cc\u7ec6\u80de\u68c0\u6d4b<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 4\uff1a\u6570\u636e\u6807\u51c6\u5316<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 5\uff1a\u7f29\u653e\u53ca\u56de\u5f52<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 6\uff1a\u7ec6\u80de\u805a\u7c7b<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>Step 7\uff1a\u7ec6\u80de\u6ce8\u91ca<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.8372093023255813\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_wZxvJu7w8OqDnW4AJp7IwSiczaNNzQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEDfselIjP3okKave9Wxz5lznVRwZxvJu7w8OqDnW4AJp7IwSiczaNNzQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"301\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<p><strong><span style=\"font-family: \u7b49\u7ebf;font-size: 10.5pt;\"><o:p><\/o:p><\/span><\/strong><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u5206\u6790\u7684\u6bcf\u4e2a\u6b65\u9aa4\u4e2d\uff0cpopsicleR \u901a\u8fc7\u5f69\u8272\u6587\u672c\u6d88\u606f\u4ea4\u4e92\u5f0f\u5730\u6307\u5bfc\u7528\u6237\uff0c\u5e76\u5728\u4e13\u7528\u6587\u4ef6\u5939\u4e2d\u4fdd\u5b58\u5404\u79cd\u56fe\uff0c\u4ee5\u7814\u7a76\u591a\u4e2a QC \u6307\u6807\u5e76\u8bc4\u4f30\u8fc7\u6ee4\u548c\u56de\u5f52\u53c2\u6570\u5bf9\u7ec6\u80de\u7fa4\u8bc6\u522b\u548c\u5206\u7c7b\u7684\u5f71\u54cd\u3002\u8dd1\u5b8c\u6d41\u7a0b\u540e\u53ef\u4e3a\u5c0f\u4f19\u4f34\u4eec\u4ea7\u51fa20\u591a\u5f20\u5404\u5f0f\u5404\u6837\u4e14\u7f8e\u89c2\u7684\u56fe\u8868\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5c0f\u679c\u8fd9\u8fb9\u5df2\u7ecf\u4e3a\u5c0f\u4f19\u4f34\u4eec\u51c6\u5907\u597d\u8f93\u5165\u6587\u4ef6\u4e86\uff0c\u83b7\u53d6\u94fe\u63a5\u653e\u5728\u6587\u7ae0\u7684\u6700\u540e\u54e6~\u5c31\u8ba9\u6211\u4eec\u6b63\u5f0f\u5f00\u59cb\u4eca\u5929\u7684\u5b66\u4e60\u5427\uff01<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"># Step 0 \u5b89\u88c5popsicleR\u5305\u53ca\u5176\u4f9d\u8d56<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u56e0\u4e3apopsicleR\u6574\u5408\u5927\u91cf\u7684R\u5305\uff0c\u56e0\u6b64\u5728\u5b89\u88c5\u4f9d\u8d56\u7684\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u6709\u4e00\u4e9b\u7e41\u7410\u54e6\uff0c\u4e0b\u9762\u7684\u4ee3\u7801\u867d\u7136\u5f88\u591a\uff0c\u4f46\u53ea\u9700\u8981\u5c0f\u4f19\u4f34\u4eec\u76f4\u63a5\u8fd0\u884c\u5c31\u53ef\u4ee5\u5566\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">options(timeout = 999) # \u65b9\u4fbf\u5728GitHub\u4e0a\u4e0b\u8f7d\u5bf9\u5e94\u7684R\u5305<\/span><span style=\"\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"\"><br \/><\/span><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\">CRANdep <- c(\"Seurat\",\"reticulate\",\"R.utils\",\"dplyr\",\"ggplot2\",\"clustree\",\"ape\",\"gtools\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"future\",\"grid\",\"gridExtra\",\"magrittr\",\"limma\",\"patchwork\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"crayon\",\"ggExtra\",\"RColorBrewer\",\"ggplotify\",\"RANN\",\"umap\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"celldex\",\"curl\",\"httr\",\"lattice\",\"shinythemes\",\"usethis\",\"rcmdcheck\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"roxygen2\",\"rversions\",\"devtools\",\"pheatmap\",\"BiocManager\",\"corrplot\")<\/span><\/code><code><span class=\"code-snippet_outer\">newPackages <- CRANdep[!(CRANdep %in% installed.packages()[,\"Package\"])]<\/span><\/code><code><span class=\"code-snippet_outer\">if(length(newPackages)){install.packages(newPackages)}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">CRANarcdep <- c(\"Matrix\",\"optimbase\",\"optimsimplex\",\"neldermead\",\"session\")<\/span><\/code><code><span class=\"code-snippet_outer\">newPackages <- CRANarcdep[!(CRANarcdep %in% installed.packages()[,\"Package\"])]<\/span><\/code><code><span class=\"code-snippet_outer\">if(length(newPackages)){<\/span><\/code><code><span class=\"code-snippet_outer\"> packagesurl <- c(\"https:\/\/cran.r-project.org\/src\/contrib\/Archive\/Matrix\/Matrix_1.3-2.tar.gz\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"https:\/\/cran.r-project.org\/src\/contrib\/Archive\/optimbase\/optimbase_1.0-9.tar.gz\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"https:\/\/cran.r-project.org\/src\/contrib\/Archive\/optimsimplex\/optimsimplex_1.0-7.tar.gz\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"https:\/\/cran.r-project.org\/src\/contrib\/Archive\/neldermead\/neldermead_1.0-11.tar.gz\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"https:\/\/cran.r-project.org\/src\/contrib\/Archive\/session\/session_1.0.3.tar.gz\")<\/span><\/code><code><span class=\"code-snippet_outer\"> for (i in 1:length(newPackages)){<\/span><\/code><code><span class=\"code-snippet_outer\"> source_repo <- packagesurl[grep(newPackages[i], packagesurl)]<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(source_repo, repos=NULL, type=\"source\")<\/span><\/code><code><span class=\"code-snippet_outer\"> }<\/span><\/code><code><span class=\"code-snippet_outer\">}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">BioCdep <- c(\"SingleR\",\"limma\",\"BiocFileCache\",\"AnnotationHub\",<\/span><\/code><code><span class=\"code-snippet_outer\"> \"ExperimentHub\",\"celldex\",\"scDblFinder\")<\/span><\/code><code><span class=\"code-snippet_outer\">newPackages <- BioCdep[!(BioCdep %in% installed.packages()[,\"Package\"])]<\/span><\/code><code><span class=\"code-snippet_outer\">if(length(newPackages)){BiocManager::install(newPackages)}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">if(!\"scMCA\"%in% installed.packages()[,\"Package\"]){devtools::install_github(\"ggjlab\/scMCA\")}<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">devtools::install_github(\"bicciatolab\/popsicleR\")<\/span><\/code><code><span class=\"code-snippet_outer\">library(popsicleR)<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u8f7d\u5165R\u5305\u540e\u51fa\u73b0<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.14285714285714285\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_kDeGxAgKvbs3CKhthCbpHDNvl4fEKQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEBRj58q3Huvx1wlicz9W0iasTiaokDeGxAgKvbs3CKhthCbpHDNvl4fEKQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5373\u4e3a\u5b89\u88c5\u6210\u529f\uff0c\u6211\u4eec\u4fbf\u53ef\u4ee5\u8fdb\u884c\u4e0b\u4e00\u6b65\u5566<\/span><span style=\"\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\"># step 1 \u4ece\u539f\u59cb\u6570\u636e\u521b\u5efa Seurat \u5bf9\u8c61\u5e76\u53ef\u89c6\u5316 QC \u6307\u6807<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- PrePlots(sample = 'CRR073027',<\/span><\/code><code><span class=\"code-snippet_outer\"> input_data = 'filtered_feature_bc_matrix\/',<\/span><\/code><code><span class=\"code-snippet_outer\"> percentage = 0.1, gene_filter = 200, cellranger = TRUE, organism = \"human\"<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"mso-spacerun:"yes";font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:"Times New Roman";\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@para sample: \u7814\u7a76\u9879\u76ee\u7684name\uff1b<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@para input_data: \u8f93\u5165\u6587\u4ef6\u7684\u8def\u5f84\uff0c\u5728\u8fd9\u9762\u5c0f\u4f19\u4f34\u4eec\u65e2\u53ef\u4ee5\u4f7f\u7528\u5c0f\u679c\u4e3a\u5927\u5bb6\u51c6\u5907\u597d\u7684cellranger\u7684\u77e9\u9635\u7ed3\u679c\uff0c\u4e5f\u53ef\u4ee5\u662f\u975ecellranger\u7684\u666e\u901a\u8ba1\u6570\u77e9\u9635\uff0c\u90fd\u662f\u517c\u5bb9\u7684\u54e6<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@para genelist: \u5728\u8fd9\u91cc\u5982\u679c\u5c0f\u4f19\u4f34\u4eec\u6709\u5c5e\u4e8e\u81ea\u5df1\u7684\u4e00\u5957\u4e0e\u7ec6\u80de\u5bf9\u5e94\u7684marker\u5217\u8868\u4e5f\u53ef\u4ee5\u4f7f\u7528\u5411\u91cf\u7684\u5f62\u5f0f\u8fdb\u884c\u6dfb\u52a0\u54e6\uff0c\u5c0f\u767d\u6ca1\u6709\u7684\u8bdd\uff0c\u4e5f\u6ca1\u6709\u5173\u7cfb\uff0c\u4f5c\u8005\u4e3a\u5927\u5bb6\u51c6\u5907\u597d\u4e86\u4e00\u5957marker\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@para percentage: \u8fd9\u91cc\u9650\u5236\u4e86\u6240\u6709\u7684\u57fa\u56e0\u8d77\u7801\u57281%\u7684\u7ec6\u80de\u4e2d\u8fdb\u884c\u8868\u8fbe\uff0c\u9ed8\u8ba4\u503c\u4e3a0.1<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@para gene_filter: \u9650\u5236\u6240\u6709\u7684\u7ec6\u80de\u6700\u8d77\u7801\u68c0\u6d4b\u51fa200\u4e2a\u57fa\u56e0<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@cellranger: \u82e5\u8f93\u5165\u6587\u4ef6\u4e3acellranger\u7684\u8f93\u51fa\u6587\u4ef6\u5219\u8bbe\u5b9a\u4e3aTRUE<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">@organism: \u9009\u62e9\u7814\u7a76\u7684\u7269\u79cd<\/span><span style=\"\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0b\u9762\u5c0f\u679c\u4e3a\u5927\u5bb6\u7b5b\u9009\u4e86\u8fd0\u884c\u5b8c\u8d28\u63a7\u7ed3\u679c\u540e\u7684\u6570\u5f20\u7ed3\u679c\u56fe\u7ed9\u5404\u4f4d\u5c0f\u4f19\u4f34\u9884\u89c8\u4e00\u4e0b<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.28715728715728717\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_zyz4UlpTHoyY1DqiaLJLkcqVcV5wbw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEhJTJN20vzkZmU1OSOaMoTDMAkzyz4UlpTHoyY1DqiaLJLkcqVcV5wbw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"693\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.6585365853658537\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_D6Ac83flSQyy2rwzPzlcTJ6sZaru3g.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkE17abgasoDXzagPaFwAJiafW8QD6Ac83flSQyy2rwzPzlcTJ6sZaru3g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"574\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.0053191489361701\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_pcxwGbeQurCnRYcPj5TCIyqM0iac6g.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkEFeichW5XtE4yE5tBCibOZJ0XB40pcxwGbeQurCnRYcPj5TCIyqM0iac6g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"376\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_opsjKibOX3JrynicW5qbsvicFiacvA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAztctFDciaTgXQTByLub6rkE9L6L0WJ6uGelrgaefjo0fgRia8cnLopsjKibOX3JrynicW5qbsvicFiacvA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"355\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"ruby\"><code><span class=\"code-snippet_outer\"># step 2 \u53cc\u91cd\u7ec6\u80de\u68c0\u6d4b<\/span><\/code><code><span class=\"code-snippet_outer\">\u8be5\u5904\u4ee3\u7801\u7684\u8fd0\u884c\u65f6\u95f4\u4f1a\u8f83\u957f\uff0c\u6709\u6761\u4ef6\u7684\u5c0f\u4f19\u4f34\u5efa\u8bae\u4f7f\u7528\u670d\u52a1\u5668\u8fdb\u884c\u8fd0\u884c\u54e6~<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- CalculateDoublets(UMI = seurat_obj, method = \"scrublet\", dbs_thr ='none', dbs_remove = FALSE)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># step 3 \u53cc\u91cd\u7ec6\u80de\u79fb\u9664<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- CalculateDoublets(UMI = seurat_obj, method = \"scrublet\", dbs_thr = 0.22, dbs_remove = TRUE)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u53c2\u6570\u8bf4\u660e:<\/span><\/code><code><span class=\"code-snippet_outer\">@para UMI: \u8f93\u5165\u7684\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u5bf9\u8c61<\/span><\/code><code><span class=\"code-snippet_outer\">@para method: \u53cc\u91cd\u7ec6\u80de\u68c0\u6d4b\u65b9\u6cd5\uff0c\u53ef\u4ee5\u9009\u62e9\"scrublet\",\"scDblFinder\"\u5176\u4e2d\u4e00\u79cd\u65b9\u5f0f<\/span><\/code><code><span class=\"code-snippet_outer\"># \u6765\u81ea\u5c0f\u679c\u7684\u6e29\u99a8\u63d0\u793a\uff0cdbs_thr\u53c2\u6570\u662f\u5728\u9009\u62e9scrublet\u65b9\u6cd5\u7684\u60c5\u51b5\u4e0b\u624d\u8fdb\u884c\u9009\u62e9\u7684\u54e6<\/span><\/code><code><span class=\"code-snippet_outer\">@para dbs_thr: \u8bbe\u5b9a\u7684\u53cc\u91cd\u7ec6\u80de\u9608\u503c\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3a'none'<\/span><\/code><code><span class=\"code-snippet_outer\">@para dbs_thr: \u8bbe\u5b9a\u7684\u53cc\u91cd\u7ec6\u80de\u9608\u503c\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3a0.22<\/span><\/code><code><span class=\"code-snippet_outer\">@para dbs_remove: \u662f\u5426\u79fb\u9664\u53cc\u91cd\u7ec6\u80de<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># step 4\u6807\u51c6\u5316<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- Normalize(UMI = seurat_obj, variable_genes = 2000)<\/span><\/code><code><span class=\"code-snippet_outer\"># \u53c2\u6570\u8bf4\u660e: <\/span><\/code><code><span class=\"code-snippet_outer\"># @para UMI: \u8f93\u5165\u7684\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u5bf9\u8c61<\/span><\/code><code><span class=\"code-snippet_outer\"># @para variable_genes: \u9009\u62e9\u7684\u53d8\u5f02\u57fa\u56e0\u6570\u91cf\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3a2000 <\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># step 5 \u56de\u5f52<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- ApplyRegression(UMI = seurat_obj, organism = \"human\", variables = \"none\", explore_PC = FALSE)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># step 6\u5bf9PC\u503c\u8fdb\u884c\u89c2\u6d4b\u540e\u8fdb\u884c\u56de\u5f52\u7814\u7a76<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- ApplyRegression(UMI = seurat_obj, organism = \"human\", variables = \"none\", explore_PC = TRUE)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u53c2\u6570\u8bf4\u660e: <\/span><\/code><code><span class=\"code-snippet_outer\">@para UMI: \u8f93\u5165\u7684\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u5bf9\u8c61<\/span><\/code><code><span class=\"code-snippet_outer\">@para organism: \u7814\u7a76\u7684\u7269\u79cd\uff0c\u8fd9\u91cc\u9009\u62e9\u4e86\"human\"<\/span><\/code><code><span class=\"code-snippet_outer\">@para variables: \u8fdb\u884c\u56de\u5f52\u5206\u6790\u7684\u53d8\u91cf\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3a\"none\"<\/span><\/code><code><span class=\"code-snippet_outer\"># \u5728\u6211\u4eec\u63a2\u7d22\u4e3b\u6210\u5206\u7684\u4e2a\u6570\u540e\u4fbf\u53ef\u5bf9\u8be5\u53c2\u6570\u8fdb\u884c\u8c03\u6574<\/span><\/code><code><span class=\"code-snippet_outer\">@para explore_PC: \u662f\u5426\u63a2\u7d22\u4e3b\u6210\u5206\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3aFALSE <\/span><\/code><code><span class=\"code-snippet_outer\">@para explore_PC: \u662f\u5426\u63a2\u7d22\u4e3b\u6210\u5206\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3aTRUE<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># step 7\u7ec6\u80de\u7c7b\u578b\u6ce8\u91ca<\/span><\/code><code><span class=\"code-snippet_outer\">seurat_obj <- MakeAnnotation(UMI = seurat_obj, organism = \"human\", marker.list = \"none\", cluster_res= 0.8)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"># \u53c2\u6570\u8bf4\u660e: <\/span><\/code><code><span class=\"code-snippet_outer\">@para UMI: \u8f93\u5165\u7684\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u5bf9\u8c61<\/span><\/code><code><span class=\"code-snippet_outer\">@para organism: \u7814\u7a76\u7684\u7269\u79cd\uff0c\u8fd9\u91cc\u9009\u62e9\u4e86\"human\"<\/span><\/code><code><span class=\"code-snippet_outer\">@para marker.list: \u7ec6\u80de\u7c7b\u578b\u6807\u8bb0\u57fa\u56e0\u5217\u8868\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3a\"none\"<\/span><\/code><code><span class=\"code-snippet_outer\">@para cluster_res: \u805a\u7c7b\u5206\u8fa8\u7387\uff0c\u8fd9\u91cc\u8bbe\u7f6e\u4e3a0.8<\/span><\/code><\/pre>\n<\/section>\n<p><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u53ef\u4ee5\u770b\u51fa\uff0c\u867d\u7136\u8fd9\u6bb5\u4ee3\u7801\u67097\u884c\uff0c\u4f46\u5b9e\u9645\u4e0a\u6709\u4e24\u884c\u4ee3\u7801\u662f\u4e3a\u4e86\u91cd\u590d\u8fd0\u884c\u4ee5\u9009\u62e9\u53c2\u6570\u7684\uff0c\u56e0\u6b64\u6211\u4eec\u53ef\u4ee5\u53ea\u75285\u884c\u4ee3\u7801\u5c31\u5b8c\u6210\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5206\u6790\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">popsicleR\u8fd9\u4e2aR\u5305\u7684\u6e90\u4ee3\u7801\u5c06\u8fd1\u67092000\u884c\uff0c\u611f\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u8fdb\u5165\u5b98\u65b9\u6587\u6863\u8fdb\u884c\u5b66\u4e60\u54e6\uff08https:\/\/github.com\/bicciatolab\/popsicleR\/\uff09\uff0c\u8fd9\u4e0d\u4ec5\u4ec5\u662f\u5bf9\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5206\u6790\u6d41\u7a0b\u7684\u5b66\u4e60\uff0c\u8fd8\u53ef\u4ee5\u901a\u8fc7\u9605\u8bfb\u6e90\u7801\u4e86\u89e3\u5230\u5404\u79cd\u5404\u6837\u7684\u8868\u683c\u7684\u7ed8\u5236\u4ee3\u7801\uff0c\u5728\u65e5\u540e\u7684\u7814\u7a76\u5b66\u4e60\u4e2d\u9887\u6709\u76ca\u5904\u3002<\/span><span style=\"text-align: center;letter-spacing: 0.034em;\"><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u597d\u5566~\u4eca\u5929\u5c0f\u679c\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\u5566\uff01\u5e0c\u671b\u4eca\u5929\u5c0f\u679c\u7684\u5206\u4eab\u53ef\u4ee5\u5e2e\u5230\u521a\u5f00\u59cb\u5165\u95e8\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5206\u6790\u7684\u5c0f\u4f19\u4f34\u4eec\u54e6~\u5982\u679c\u5c0f\u4f19\u4f34\u6709\u5176\u4ed6\u6570\u636e\u5206\u6790\u9700\u6c42\uff0c\u53ef\u4ee5\u5c1d\u8bd5\u4f7f\u7528\u672c\u516c\u53f8\u65b0\u5f00\u53d1\u7684\u751f\u4fe1\u5206\u6790\u5c0f\u5de5\u5177\u4e91\u5e73\u53f0\uff0c\u96f6\u4ee3\u7801\u5b8c\u6210\u5206\u6790\uff0c\u975e\u5e38\u65b9\u4fbf\u5965\uff0c\u4e91\u5e73\u53f0\u7f51\u5740\u4e3a\uff1a(<\/span><span style=\"color: rgb(5, 99, 193);text-decoration: underline;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">http:\/\/www.biocloudservice.com\/home.html<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">)\uff0c\u5176\u4e2d\u4e5f\u5305\u62ec\u4e86\u901a\u8def\u8868\u8fbe\u5206\u6790(<\/span><span style=\"color: rgb(5, 99, 193);text-decoration: underline;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">http:\/\/www.biocloudservice.com\/313\/313.php<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">)\uff0c\u5355\u7ec6\u80de\u7684\u57fa\u56e0\u5171\u8868\u8fbe\u5206\u6790(<\/span><span style=\"color: rgb(5, 99, 193);text-decoration: underline;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">http:\/\/www.biocloudservice.com\/906\/906.php<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">)\u7b49\u5404\u79cd\u5c0f\u5de5\u5177\u54e6~\uff0c\u6709\u5174\u8da3\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u767b\u5f55\u7f51\u7ad9\u8fdb\u884c\u4e86\u89e3\u3002<\/span><span style=\"\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"color: rgb(255, 0, 0);font-size: 10.5pt;background: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\u4e91\u751f\u4fe1\u5e73\u53f0\u94fe\u63a5\uff1a<\/span><span style=\"text-decoration: underline;font-family: \u5fae\u8f6f\u96c5\u9ed1;color: rgb(255, 0, 0);background: rgb(255, 255, 255);\">http:\/\/www.biocloudservice.com\/home.html\u3002<\/span><span style=\"mso-spacerun:"yes";font-family:\u5fae\u8f6f\u96c5\u9ed1;color:rgb(255,0,0);font-size:10.5000pt;mso-font-kerning:1.0000pt;background:rgb(255,255,255);mso-shading:rgb(255,255,255);\"><o:p><\/o:p><\/span><\/p>\n<p><span 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