<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 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#e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid 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solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"bioawk\u4e00\u4e2a\u8d85\u597d\u7528\u751f\u4fe1\u6587\u672c\u5904\u7406\u8f6f\u4ef6\",\"time\":\"2023-09-30 19:00:08\",\"timeStamp\":\"1696071608\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493710&idx=1&sn=649a6508cedbd3011243b5f144cff9ac&chksm=cf7f788bf808f19d7222d932261c1e00449658c0f7c5350ac449f2c1a6a7f5e357bd2535ae24#rd\" target=\"_blank\" rel=\"noopener\">bioawk\u4e00\u4e2a\u8d85\u597d\u7528\u751f\u4fe1\u6587\u672c\u5904\u7406\u8f6f\u4ef6<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-09-30 19:00:08<\/em><\/div>\n<p><content><\/p>\n<div class=\"topic\">\n<p>\u6536\u5f55\u4e8e\u8bdd\u9898<\/p>\n<p><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2664545260517294084#wechat_redirect\" title=\"\u751f\u4fe1\u5b9e\u64cd\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5b9e\u64cd<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3138206666342268940#wechat_redirect\" title=\"\u751f\u4fe1\u5c0f\u767d\u76f8\u5173\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5c0f\u767d\u76f8\u5173<\/a><\/p>\n<\/div>\n<section data-mpa-powered-by=\"yiban.io\">\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section powered-by=\"xiumi.us\">\n<section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;\">\u4eca\u5929\u5c0f\u679c\u7ed9\u5927\u5bb6\u5206\u4eab\u7684\u662f\uff1a<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;\">bioawk\uff0c\u975e\u5e38\u597d\u7528\u7684\u5de5\u5177\uff0c\u52a9\u529b\u60a8\u66f4\u597d\u66f4\u5feb\u5730\u5904\u7406\u751f\u4fe1\u6570\u636e\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;\">awk\u662flinux\u7cfb\u7edf\u4e2d\u5e38\u7528\u7684\u5904\u7406\u6587\u672c\u6587\u4ef6\u7684\u8bed\u8a00\uff0c\u5b83\u63d0\u4f9b\u4e00\u4e2a\u7c7b\u7f16\u7a0b\u73af\u5883\u6765\u4fee\u6539\u548c\u91cd\u65b0\u7ec4\u7ec7\u6587\u4ef6\u4e2d\u7684\u6570\u636e\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;\">\u56e0\u4e3a\u5176\u529f\u80fd\u5f3a\u5927\uff0c\u80fd\u591f\u72ec\u5f53\u4e00\u9762\u800c\u5e7f\u53d7\u4f7f\u7528\u8005\u7684\u597d\u8bc4\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;\">\u4e4b\u6240\u4ee5\u53eb awk \u662f\u56e0\u4e3a\u5176\u53d6\u4e86\u4e09\u4f4d\u521b\u59cb\u4eba Alfred Aho\uff0cPeter Weinberger, \u548c Brian Kernighan \u7684 Family Name \u7684\u9996\u5b57\u7b26\u3002<\/span><span style=\"text-align: left;letter-spacing: 0.034em;\"><\/span><\/p>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u751f\u4fe1\u9886\u57df\uff0c\u6570\u636e\u683c\u5f0f\u590d\u6742\u591a\u6837\uff0c\u5bf9\u5176\u6570\u636e\u6587\u672c\u7684\u5904\u7406\u4ea7\u751f\u4e86\u65b0\u7684\u6311\u6218\u3002awk\u867d\u7136\u4e5f\u80fd\u505a\uff0c\u4f46\u662f\u7528\u8d77\u6765\u7ec8\u7a76\u8fd8\u662f\u6ca1\u6709\u90a3\u4e48\u5f97\u5fc3\u5e94\u624b\u3002\u5e7f\u5927\u751f\u4fe1\u4eba\u5c31\u60f3\uff0c\u8981\u662f\u80fd\u6709\u4e2a\u4e13\u95e8\u5904\u7406\u751f\u4fe1\u6570\u636e\u6587\u4ef6\u7684awk\u8be5\u6709\u591a\u597d\u554a~<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u751f\u4fe1\u9886\u57df\u7684\u5927\u725b\u674e\u6052\uff0c\u6293\u4f4f\u4e86\u8fd9\u4e00\u75db\u70b9\uff0c\u52a8\u624b\u5bf9awk\u7684\u6e90\u7801\u8fdb\u884c\u4e86\u6539\u9020\u3002\u4e13\u95e8\u9488\u5bf9\u751f\u4fe1\u9886\u57df\u7684\u6587\u672c\u5904\u7406\u5de5\u5177\u2014\u2014bioawk\u5c31\u7531\u6b64\u5e94\u8fd0\u800c\u751f\u4e86\u3002\u800c\u4e14\u662f\u9752\u51fa\u4e8e\u84dd\u800c\u80dc\u4e8e\u84dd\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u505a\u751f\u4fe1\u7684\u4f60\u8fd8\u6ca1\u7528\u8fc7\u7684\u8bdd\uff0c\u8d76\u7d27\u6765\u8ddf\u5c0f\u679c\u4e0a\u624b\u5427~<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<h2 style=\"text-align: center;\"><em><span style=\"font-size: 20px;\"><strong><span style=\"font-family: \u9ed1\u4f53;\">\u8f6f\u4ef6\u5b89\u88c5<\/span><\/strong><\/span><\/em><strong><span style=\"mso-spacerun:'yes';font-family:\u9ed1\u4f53;mso-ascii-font-family:Arial;mso-hansi-font-family:Arial;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:16.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h2>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5c0f\u679c\u63a8\u8350\u7528conda\u5b89\u88c5<\/span><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<h1 style=\"text-align: center;\"><em style=\"letter-spacing: 0.034em;\"><\/em><\/h1>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\">conda install -y bioawk<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">\u5f53\u7136\u4e5f\u53ef\u4e0b\u8f7d\u6e90\u7801\u7f16\u8bd1<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> sudo apt-get install byacc git <span class=\"code-snippet__comment\">#\u5b89\u88c5git<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> git <span class=\"code-snippet__built_in\">clone<\/span> git:\/\/github.com\/lh3\/bioawk.git <span class=\"code-snippet__comment\">#\u9ed8\u8ba4\u5b89\u88c5\u5728\u5f53\u524d\u76ee\u5f55<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> git <span class=\"code-snippet__built_in\">clone<\/span> git:\/\/github.com\/lh3\/bioawk.git <span class=\"code-snippet__variable\">${path}<\/span>\/bioawk <span class=\"code-snippet__comment\">#\u82e5\u52a0path\u5219\u5fc5\u987b\u4e3a\u7a7a\uff0c\u56e0\u6b64\u53ef\u5728\u60f3\u8981\u5b89\u88c5\u7684\u76ee\u5f55\u4e0b\u8d77\u4e00\u4e2a\u65b0\u540d\u79f0\uff0cgit\u4f1aclone\u5230\u5176\u4e0b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__built_in\">cd<\/span> bioawk<\/span><\/code><code><span class=\"code-snippet_outer\"> make<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__built_in\">echo<\/span> <span class=\"code-snippet__string\">\"export PATH=<span class=\"code-snippet__variable\">${path}<\/span>\/bioawk\/:${PATH}\"<\/span> >> ~\/.bashrc <\/span><\/code><code><span class=\"code-snippet_outer\"> . ~\/.bashrc <\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" data-type=\"jpeg\" data-w=\"1080\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_IlmBAWe69EMUPMhNEgrv6NXX85lJxg-22.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwyrj7kbUR3ULG9ZUbLrLkgibzL8qUpQROfe8ebc3gNSnu1dIlmBAWe69EMUPMhNEgrv6NXX85lJxg\/640?wx_fmt=jpeg'\" data-recalc-dims=\"1\" \/><\/p>\n<h2 style=\"text-align: center;\"><span style=\"font-size: 20px;\"><em><strong><span style=\"font-family: \u9ed1\u4f53;\"><span style=\"font-family: Arial;\">Bioawk<\/span>\u7684\u4f7f\u7528<\/span><\/strong><\/em><\/span><strong><span style=\"mso-spacerun:'yes';font-family:\u9ed1\u4f53;mso-ascii-font-family:Arial;mso-hansi-font-family:Arial;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:16.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h2>\n<h3><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>\u4e00\u3001\u5e2e\u52a9\u6587\u6863\u4e0e\u4f7f\u7528\u65b9\u6cd5<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/h3>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u9996\u5148\u6211\u4eec\u67e5\u770b\u4e00\u4e0b\u5e2e\u52a9\u6587\u6863\uff0c<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\">#\u67e5\u770b\u5e2e\u52a9\u6587\u6863<\/span><\/code><code><span class=\"code-snippet_outer\">bioawk -c help<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.16931982633863965\" data-s=\"300,640\" data-type=\"png\" data-w=\"691\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_NGCPdq2wHgmq6HwLKgHiaH0b7N0xxA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOictcnajbxgHBT1Sjf0q4eBa7fOrNGCPdq2wHgmq6HwLKgHiaH0b7N0xxA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code><span class=\"code-snippet_outer\">#bioawk\u7684\u9009\u9879\u53c2\u6570\uff0c\u4f7f\u7528\u65b9\u6cd5<\/span><\/code><code><span class=\"code-snippet_outer\">bioawk<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.044797687861271675\" data-s=\"300,640\" data-type=\"png\" data-w=\"692\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_WKRTytjqBIAHzktVKq3MUEKxUTpTrg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOicKMGEx17icecyfvrF8sCJk16FPWKRTytjqBIAHzktVKq3MUEKxUTpTrg\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u63a5\u4e0b\u91cc\u662f\u70b9\u56fe#\u70b9\u56fe<\/span><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">-F: \u6307\u5b9a\u5217\u4e0e\u5217\u4e4b\u95f4\u7684\u5206\u9694\u7b26\uff1b<\/span><\/code><code><span class=\"code-snippet_outer\">-c: \u652f\u6301\u7684\u6587\u4ef6\u683c\u5f0f\uff1b<\/span><\/code><code><span class=\"code-snippet_outer\">-v: \u8bbe\u7f6e\u81ea\u5b9a\u4e49\u7684\u53d8\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">-f: \u4ece\u6307\u5b9a\u7684\u6587\u4ef6\u4e2d\u8bfb\u53d6\u7a0b\u5e8f<\/span><\/code><code><span class=\"code-snippet_outer\">[file]: \u6700\u540e\u6307\u5b9a\u7684\u64cd\u4f5c\u6587\u4ef6\u3002<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\">\u63a5\u4e0b\u6765\u5c0f\u679c\u4f1a\u5e26\u5927\u5bb6\u7531\u6d45\u81f3\u6df1\u5b66\u4e60<span style=\"font-family:Calibri;\">bioawk<\/span>\u7684\u4f7f\u7528\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: \u5b8b\u4f53;\"><br \/><\/span><\/p>\n<h3><span style=\"font-size: 14px;\"><strong><span style=\"font-family: \u5b8b\u4f53;\">\u4e8c\u3001\u5904\u7406<span style=\"font-family: Calibri;\">fasta<\/span>\u5e8f\u5217\u6587\u4ef6<\/span><\/strong><strong><span style=\"font-family: Calibri;\"><o:p><\/o:p><\/span><\/strong><\/span><\/h3>\n<h4><span style=\"font-size: 14px;\"><span style=\"font-family: \u9ed1\u4f53;\">\uff08\u4e00\uff09<\/span><span style=\"font-family: Arial;\"><span style=\"font-family: \u9ed1\u4f53;\">\u6253\u5370<\/span>fasta<span style=\"font-family: \u9ed1\u4f53;\">\u5e8f\u5217<\/span>ID<span style=\"font-family: \u9ed1\u4f53;\">\u3001<\/span><\/span><span style=\"font-family: \u9ed1\u4f53;\">\u5e8f\u5217<\/span><span style=\"font-family: Arial;\"><span style=\"font-family: \u9ed1\u4f53;\">\u957f\u5ea6\u3001<\/span>GC<span style=\"font-family: \u9ed1\u4f53;\">\u542b\u91cf<\/span><\/span><\/span><strong><span style=\"mso-spacerun:'yes';font-family:Arial;mso-fareast-font-family:\u9ed1\u4f53;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:14.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h4>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">bioawk -cfastx '{print\" ID:\"$name\" t length:\"length($seq)\" t GC:\"gc($seq)\" \"}' xxx.fna<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">-c fastx: \u6307\u5b9a\u6587\u4ef6\u4e3afastx\u683c\u5f0f\uff0c\u5305\u62ecfasta\uff0cfastq<\/span><\/code><code><span class=\"code-snippet_outer\">\u2018{code}\u2019: \u4ee3\u7801\u9700\u8981\u7528{}\u62ec\u8d77\u6765<\/span><\/code><code><span class=\"code-snippet_outer\">$name: \u53d6\u6587\u4ef6\u4e2d\u7684\u53d8\u91cfname<\/span><\/code><code><span class=\"code-snippet_outer\">$seq: \u53d6\u6587\u4ef6\u4e2d\u7684\u5e8f\u5217<\/span><\/code><code><span class=\"code-snippet_outer\">length(): length\u51fd\u6570\u7edf\u8ba1\u5e8f\u5217\u957f\u5ea6<\/span><\/code><code><span class=\"code-snippet_outer\">gc(): \u5e8f\u5217\u4e2d\u7684gc\u542b\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">XXX.fna: \u6307\u5b9a\u7684\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">t: \u4e24\u5217\u4fe1\u606f\u7528\u5236\u8868\u7b26t\u5206\u5272<\/span><\/code><code><span class=\"code-snippet_outer\">\u2018 \u2019: \u811a\u672c{}\u88ab\u89c6\u4e3a\u5355\u4e2a\u6587\u672c\u5b57\u7b26\u4e32\u9700\u8981\u2019\u2019\u62ec\u8d77\u6765\uff0c\u5e76\u4e14\u5355\u5f15\u53f7\u8868\u793a\u8f93\u5165\u7684\u8d77\u59cb\u4e0e\u7ed3\u675f\u3002<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.1199421965317919\" data-s=\"300,640\" data-type=\"png\" data-w=\"692\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_QS8iazLoRWxhibia63ySVH2bPaoowA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOicfMABqdQYzpCRAV7k2iapR3B8yc3HQS8iazLoRWxhibia63ySVH2bPaoowA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\">\u8f93\u5165\u4ee3\u7801\u5373\u53ef\u6253\u5370\u7ed3\u679c\u5230\u7ec8\u7aef\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><br \/><\/o:p><\/span><\/p>\n<h4><span style=\"font-size: 14px;\"><strong><span style=\"font-family: \u9ed1\u4f53;\">\uff08<\/span><\/strong><strong><span style=\"font-family: \u9ed1\u4f53;\">\u4e8c<\/span><\/strong><strong><span style=\"font-family: \u9ed1\u4f53;\">\uff09<\/span><\/strong><strong><span style=\"font-family: \u9ed1\u4f53;\">\u83b7\u53d6<\/span><\/strong><strong><span style=\"font-family: Arial;\">fasta<\/span><\/strong><strong><span style=\"font-family: \u9ed1\u4f53;\">\u5e8f\u5217\u7684\u53cd\u5411\u4e92\u8865\u94fe\u5e8f\u5217<\/span><\/strong><\/span><\/h4>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8fd9\u662f\u793a\u4f8b\u6587\u4ef6demo.fasta,\u7684\u6570\u636e<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<h4><strong><span style=\"mso-spacerun:'yes';font-family:Arial;mso-fareast-font-family:\u9ed1\u4f53;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:14.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h4>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3\" data-s=\"300,640\" data-type=\"png\" data-w=\"200\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_UYaibiafliadey4JzSZP2urZXeWcVQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOicLr3gNDXPll0ibYxZ0zhyKFrCxNtDUYaibiafliadey4JzSZP2urZXeWcVQ\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\">#\u8f93\u51fa\u793a\u4f8b\u6570\u636e\u76845\u2019->3\u2019\u53cd\u5411\u4e92\u8865\u5e8f\u5217<\/span><\/code><code><span class=\"code-snippet_outer\">bioawk -c fastx '{ print \">\" $name;print revcomp($seq) }' demo.fasta > revdemo.fasta<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">\u4f7f\u7528revcomp()\u51fd\u6570\u5373\u53ef\u5f97\u52305\u2019->3\u2019\u53cd\u5411\u4e92\u8865\u5e8f\u5217<\/span><\/code><code><span class=\"code-snippet_outer\">revcomp($seq): \u62ec\u53f7\u4e2d\u8f93\u5165\u9700\u8981\u5904\u7406\u7684\u5e8f\u5217\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">> revdemo.fasta: \u5c06\u7ed3\u679c\u8f93\u51fa\u5230\u65b0\u7684fasta\u6587\u4ef6 revdemo\u4e2d<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3005464480874317\" data-s=\"300,640\" data-type=\"png\" data-w=\"183\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_JaOAwtIQ2LIBK7brz9egAic4sRhQGA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOicdYhIyKVrABabiab6gNicdfX3vaOJaOAwtIQ2LIBK7brz9egAic4sRhQGA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0a\u8ff0\u7684\u5355\u56fe\u662f\u4e0d\u662f\u90fd\u5f88\u719f\u6089\uff0c\u90fd\u662f\u5e73\u65f6\u6211\u4eec\u505a\u7684\u6bd4\u8f83\u591a\u7684\u56fe\uff0c\u6211\u4eec\u521b\u5efa\u5b8c\u6210\u540e\uff0c\u5c31\u5f00\u59cb\u7ed8\u5236\u7ec4\u5408\u56fe\u7247\u628a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u8fd9\u91cc\u4f7f\u7528ggpubr\u5305\u4e2d\u51fd\u6570ggarrange\uff08\uff09\u5728\u4e00\u9875\u4e0a\u8fdb\u884c\u5c55\u793a\u4e0a\u8ff0\u7684\u7ed3\u679c<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5bf9ToothGrowth\u6570\u636e\u96c6\u7684\u7bb1\u7ebf\u56fe\uff0c\u70b9\u56fe\u7ec4\u5408\u5c55\u793a\uff1a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">ggarrange(Box_plot, Dot_plot,labels = c(“A”, “B”),ncol = 2, nrow = 1)<\/span><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<h3><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>\u4e09\u3001\u5904\u7406fastq\u6587\u4ef6<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/h3>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u6307\u5b9a\u7684\u6587\u4ef6\u683c\u5f0f\u4f9d\u7136\u9009\u62e9 -c fastx\uff0c\u5b83\u80fd\u591f\u81ea\u52a8\u8bc6\u522b\u5e8f\u5217\u662ffastq \u8fd8\u662f fasta\u3002Fastq\u6587\u4ef6\u6bd4fasta\u6587\u4ef6\u591a\u4e86\u6d4b\u5e8f\u8d28\u91cf\u7684\u4fe1\u606f\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u6211\u4eec\u7528\u4e8e\u6f14\u793a\u7684fastq\u90e8\u5206\u6587\u4ef6\u5982\u4e0b\uff1a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u662f\u4e0d\u662f\u5c31\u5b8c\u6210\u4e86\u5462\uff0cAB\u5e8f\u53f7\u5c0f\u4f19\u4f34\u53ef\u4ee5\u81ea\u884c\u8c03\u6574<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u540e\u9762\u6211\u4eec\u5bf9#mtcars \u6570\u636e\u96c6\u7684\u6761\u5f62\u56fe\uff0c\u6563\u70b9\u56fe\u7ec4\u5408\u5c55\u793a<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2922173274596182\" data-s=\"300,640\" data-type=\"png\" data-w=\"681\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_AAtfDibNDzNslqzyzawSI3v0vIhicQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOictPmadtTwcyUgGwiblFxDs71AkqIAAtfDibNDzNslqzyzawSI3v0vIhicQ\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<h4><span style=\"font-size: 14px;\"><strong><span style=\"font-family: \u9ed1\u4f53;\">\uff08<\/span><\/strong><strong><span style=\"font-family: \u9ed1\u4f53;\">\u4e00<\/span><\/strong><strong><span style=\"font-family: \u9ed1\u4f53;\">\uff09<\/span><\/strong><strong><span style=\"font-family: Arial;\"><span style=\"font-family: \u9ed1\u4f53;\">\u8ba1\u7b97<\/span>fastq<span style=\"font-family: \u9ed1\u4f53;\">\u5e8f\u5217\u7684\u884c\u6570<\/span><\/span><\/strong><strong><span style=\"font-family: Arial;\"><o:p><\/o:p><\/span><\/strong><\/span><\/h4>\n<p><span style=\"font-family: \u5b8b\u4f53;font-size: 14px;\">\u6784\u5efa\u6d4b\u5e8f\u6570\u636e\u5206\u6790 <span style=\"font-family: Calibri;\">pipeline <\/span>\u65f6\uff0c\u5f53<\/span><span style=\"font-family: \u5b8b\u4f53;font-size: 14px;\">fastq<\/span><span style=\"font-family: \u5b8b\u4f53;font-size: 14px;\"><span style=\"font-family: Calibri;\"> <\/span>\u6570\u636e\u5728\u505a\u5b8c <span style=\"font-family: Calibri;\">trimming <\/span>\u540e\uff0c\u6211\u4eec\u5f80\u5f80\u8981\u5173\u6ce8\u5269\u4e0b\u591a\u5c11 <span style=\"font-family: Calibri;\">reads<\/span>\uff0c\u8fd9\u662f\u53ef\u4ee5\u7528<span style=\"font-family: Calibri;\"> <\/span><\/span><span style=\"font-family: \u5b8b\u4f53;font-size: 14px;\">bioawk<\/span><span style=\"font-family: \u5b8b\u4f53;font-size: 14px;\"><span style=\"font-family: Calibri;\"> <\/span>\u8fdb\u884c\u5feb\u901f\u7edf\u8ba1\u3002<\/span><\/p>\n<p><span style=\"font-family: \u5b8b\u4f53;font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.06791907514450866\" data-s=\"300,640\" data-type=\"png\" data-w=\"692\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_ATXN906XYxnelicdrSuIdTOwPqPiaA.png?w=640\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-30_bioawk\u4e00\u4e2a\u8d85\u597d\u7528\u751f\u4fe1\u6587\u672c\u5904\u7406\u8f6f\u4ef6\/9_ATXN906XYxnelicdrSuIdTOwPqPiaA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOic9iafaUtCHMvOica3EoOjT4yyU5A5ATXN906XYxnelicdrSuIdTOwPqPiaA\/640?wx_fmt=png''\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\">bioawk -c fastx 'END {print NR}' SRR1039509_2.fastq<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.06791907514450866\" data-s=\"300,640\" data-type=\"png\" data-w=\"692\" style=\"font-size: 16px;letter-spacing: 0.578px;text-align: center;height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_ATXN906XYxnelicdrSuIdTOwPqPiaA.png?w=640\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-30_bioawk\u4e00\u4e2a\u8d85\u597d\u7528\u751f\u4fe1\u6587\u672c\u5904\u7406\u8f6f\u4ef6\/9_ATXN906XYxnelicdrSuIdTOwPqPiaA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOic9iafaUtCHMvOica3EoOjT4yyU5A5ATXN906XYxnelicdrSuIdTOwPqPiaA\/640?wx_fmt=png''\" data-recalc-dims=\"1\" \/><\/p>\n<p><br \/><\/p>\n<p><br \/><\/p>\n<h4><strong><span style=\"mso-spacerun:'yes';font-family:\u9ed1\u4f53;mso-ascii-font-family:Arial;mso-hansi-font-family:Arial;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:14.0000pt;mso-font-kerning:1.0000pt;\">\uff08\u4e8c\uff09\u7edf<\/span><\/strong><strong><span style=\"mso-spacerun:'yes';font-family:Arial;mso-fareast-font-family:\u9ed1\u4f53;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:14.0000pt;mso-font-kerning:1.0000pt;\"><span style=\"font-family:\u9ed1\u4f53;\">\u8ba1\u7279\u6b8a\u78b1\u57fa\u5f00\u5934\u7684<\/span> reads <span style=\"font-family:\u9ed1\u4f53;\">\u6570<\/span><\/span><\/strong><strong><span style=\"mso-spacerun:'yes';font-family:Arial;mso-fareast-font-family:\u9ed1\u4f53;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:14.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h4>\n<p><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><span style=\"font-family:\u5b8b\u4f53;\">\u5728\u4e00\u4e9b\u7279\u6b8a\u573a\u5408\u91cc\uff0c\u9700\u8981\u5206\u6790<\/span> reads <span style=\"font-family:\u5b8b\u4f53;\">\uff0c\u7528<\/span><\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\">bioawk<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"> <span style=\"font-family:\u5b8b\u4f53;\">\u53ef\u4ee5\u5f88\u65b9\u4fbf\u5feb\u901f\u6765\u5b8c\u6210\u3002\u6bd4\u5982\u7edf\u8ba1\u7279\u6b8a\u78b1\u57fa\u5f00\u5934\u7684 <\/span>reads <span style=\"font-family:\u5b8b\u4f53;\">\u6570\u3002<\/span><o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"># <span style=\"font-family:\u5b8b\u4f53;\">\u7edf\u8ba1\u4ee5<\/span>GATTAC<span style=\"font-family:\u5b8b\u4f53;\">\u5f00\u5934\u7684<\/span>reads<span style=\"font-family:\u5b8b\u4f53;\">\u7684\u6570\u91cf<\/span><o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\">bioawk -c fastx ‘$seq~\/^GATTAC\/ {++n} END { print n }’ my_fastq.tar.gz<o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"># \u7edf\u8ba1\u4ee5GATTAC\u5f00\u5934\u7684reads\u7684\u6570\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">bioawk -c fastx '$seq~\/^GATTAC\/ {++n} END { print n }' my_fastq.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><br \/><\/p>\n<h4><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\uff08\u4e09\uff09<strong>\u67e5\u770b<\/strong><strong><span style=\"font-family: Arial;\">GC\u542b\u91cf\u5927\u4e8e60%\u7684reads<span style=\"font-family: \u9ed1\u4f53;\">\u7684\u8d28\u91cf\u60c5\u51b5<\/span><\/span><\/strong><\/span><\/h4>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">bioawk<\/span> -c fastx <span class=\"code-snippet__string\">'{if (gc(<span class=\"code-snippet__variable\">$seq<\/span>)>0.6) printf \"%sn%snn\", <span class=\"code-snippet__variable\">$seq<\/span>, <span class=\"code-snippet__variable\">$qual<\/span>}'<\/span> my_fastq.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<p style=\"margin-bottom: 0px;font-size: 16px;letter-spacing: 0.578px;text-align: left;text-wrap: wrap;\"><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8fd9\u91cc\u4f7f\u7528\u4e86if (gc($seq)>0.6) \u6761\u4ef6\u5224\u65ad\u8bed\u53e5\uff0c\u6761\u4ef6\u662fgc\u542b\u91cf\u5927\u4e8e60%\u3002<o:p><\/o:p><\/span><\/p>\n<p style=\"margin-bottom: 0px;font-size: 16px;letter-spacing: 0.578px;text-align: left;text-wrap: wrap;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u6253\u5370printf\uff0c\u5e8f\u5217 $seq\u4ee5\u53ca\u6d4b\u5e8f\u8d28\u91cf$qual<\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<h4><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>\uff08\u56db\uff09\u8ba1\u7b97\u5e8f\u5217\u5e73\u5747\u8d28\u91cf\u5206\u6570<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/h4>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">bioawk -c fastx ‘{print “>”$name; print meanqual($qual)}’ SRR1039509_2.fastq<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u6211\u4eec\u53ef\u4ee5\u8fd0\u884cbioawk\u8ba1\u7b97\u6bcf\u6761\u5e8f\u5217\u7684\u5e73\u5747\u8d28\u91cf\u5206\u6570,\u901a\u8fc7\u51fd\u6570meanqual()\u6765\u5b9e\u73b0\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.0833333333333333\" data-s=\"300,640\" data-type=\"png\" data-w=\"252\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_lE4JF4zyhqLAFTbcicKYF5U7NVdRHA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOicblOZTibO7bECvwtszcl9rnZRibzlE4JF4zyhqLAFTbcicKYF5U7NVdRHA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8fd4\u56de\u5e8f\u5217\u7684\u540d\u79f0\uff0c\u4ee5\u53ca\u8d28\u91cf\u5206\u6570\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<h4><span style=\"font-size: 14px;\"><strong><span style=\"font-family: \u9ed1\u4f53;\"><span style=\"font-family: \u9ed1\u4f53;\">\uff08\u4e94\uff09\u5c06<\/span><span style=\"font-family: Arial;\">fastq<\/span><span style=\"font-family: \u9ed1\u4f53;\">\u683c\u5f0f\u8f6c\u6362\u4e3a<\/span><span style=\"font-family: Arial;\">fasta<\/span><span style=\"font-family: \u9ed1\u4f53;\">\u683c\u5f0f<\/span><\/span><\/strong><\/span><strong><span style=\"mso-spacerun:'yes';font-family:\u9ed1\u4f53;mso-ascii-font-family:Arial;mso-hansi-font-family:Arial;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:14.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h4>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">bioawk<\/span> -c fastx <span class=\"code-snippet__string\">'{print \">\"<span class=\"code-snippet__variable\">$name<\/span>; print <span class=\"code-snippet__variable\">$seq<\/span>}'<\/span> SRR1039509_2.fastq<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3598265895953757\" data-s=\"300,640\" data-type=\"png\" data-w=\"692\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_tLSmQ0c4BHpClljchdxickuYtr4CkA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOicmn4rgawE2OYouUibmIiapN2Ib6QtLSmQ0c4BHpClljchdxickuYtr4CkA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><\/span><\/p>\n<p style=\"line-height: normal;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans 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"Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;color: rgb(0, 0, 0);\">\u5c0f\u4f19\u4f34\u4eec\uff0c\u4eca\u5929\u6709\u6ca1\u6709\u5b66\u5230\u65b0\u77e5\u8bc6\u5462\uff0c\u60f3\u8981\u7ee7\u7eed\u4e86\u89e3R\u8bed\u8a00\u5185\u5bb9\u53ef\u4ee5\u6301\u7eed\u5173\u6ce8\u5c0f\u679c\u54e6\uff5e\u6216\u8005\u4e5f\u53ef\u4ee5\u5173\u6ce8\u6211\u4eec\u7684\u5b98\u7f51\u4e5f\u4f1a\u6301\u7eed\u66f4\u65b0\u7684\u54e6\uff5e<\/span><strong style=\"letter-spacing: 0.578px;font-size: var(--articleFontsize);\"><\/strong><\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"width: 520px;\">\n<section style=\"border-top: 1px solid rgb(0, 0, 0);width: 519.479px;float: left;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"margin-top: -3px;width: 6px;height: 6px;border-radius: 100%;float: right;background-color: rgb(46, 172, 109);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: -1em;padding: 3px 10px;display: inline-block;vertical-align: top;background-color: rgb(255, 255, 255);color: rgb(0, 154, 68);\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/a><br \/><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491909&idx=1&sn=902e34bcf53e605fba2b457ec6a33e36&chksm=cf7f7180f808f8961542f458a49da14e85d8831449fb65fbf2623d55affc564686a5422f56fd&scene=21#wechat_redirect\" textvalue=\"3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8<\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491905&idx=1&sn=fb34ebd6282962c7803badaee7a39096&chksm=cf7f7184f808f8929608c0050344ad86193d0fc6455c1c18e16cc17bf4b37832b63d3b18284e&scene=21#wechat_redirect\" textvalue=\"4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b<\/a><\/section>\n<p><br \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: center;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247490130&idx=1&sn=1578a7fab283ba24e1ce5cd6087e2d0d&chksm=cf7c8a97f80b038111f1d3df359dbee67a49462b61b5bfed1060dd83c5d7b975f0db40f4626a&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.4255555555555556\" data-s=\"300,640\" data-type=\"jpeg\" data-w=\"900\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_3enDMf2LD6pjia3zQolKXIfTwuh6AA.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxweqRKIWbRUBSWSGID9aog3q3QVgsHZplb1JyboXh8CPz6b3enDMf2LD6pjia3zQolKXIfTwuh6AA\/640?wx_fmt=jpeg'\" data-recalc-dims=\"1\" \/><\/span><\/a><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" data-type=\"jpeg\" data-w=\"1080\" style=\"height: auto !important;\" 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[…]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/59708"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=59708"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/59708\/revisions"}],"predecessor-version":[{"id":59721,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/59708\/revisions\/59721"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=59708"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=59708"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=59708"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

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solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"bioawk\u4e00\u4e2a\u8d85\u597d\u7528\u751f\u4fe1\u6587\u672c\u5904\u7406\u8f6f\u4ef6\",\"time\":\"2023-09-30 19:00:08\",\"timeStamp\":\"1696071608\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493710&idx=1&sn=649a6508cedbd3011243b5f144cff9ac&chksm=cf7f788bf808f19d7222d932261c1e00449658c0f7c5350ac449f2c1a6a7f5e357bd2535ae24#rd\" target=\"_blank\" rel=\"noopener\">bioawk\u4e00\u4e2a\u8d85\u597d\u7528\u751f\u4fe1\u6587\u672c\u5904\u7406\u8f6f\u4ef6<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-09-30 19:00:08<\/em><\/div>\n<p><content><\/p>\n<div class=\"topic\">\n<p>\u6536\u5f55\u4e8e\u8bdd\u9898<\/p>\n<p><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2664545260517294084#wechat_redirect\" title=\"\u751f\u4fe1\u5b9e\u64cd\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5b9e\u64cd<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3138206666342268940#wechat_redirect\" title=\"\u751f\u4fe1\u5c0f\u767d\u76f8\u5173\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5c0f\u767d\u76f8\u5173<\/a><\/p>\n<\/div>\n<section data-mpa-powered-by=\"yiban.io\">\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section powered-by=\"xiumi.us\">\n<section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;\">\u4eca\u5929\u5c0f\u679c\u7ed9\u5927\u5bb6\u5206\u4eab\u7684\u662f\uff1a<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;\">bioawk\uff0c\u975e\u5e38\u597d\u7528\u7684\u5de5\u5177\uff0c\u52a9\u529b\u60a8\u66f4\u597d\u66f4\u5feb\u5730\u5904\u7406\u751f\u4fe1\u6570\u636e\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;\">awk\u662flinux\u7cfb\u7edf\u4e2d\u5e38\u7528\u7684\u5904\u7406\u6587\u672c\u6587\u4ef6\u7684\u8bed\u8a00\uff0c\u5b83\u63d0\u4f9b\u4e00\u4e2a\u7c7b\u7f16\u7a0b\u73af\u5883\u6765\u4fee\u6539\u548c\u91cd\u65b0\u7ec4\u7ec7\u6587\u4ef6\u4e2d\u7684\u6570\u636e\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;\">\u56e0\u4e3a\u5176\u529f\u80fd\u5f3a\u5927\uff0c\u80fd\u591f\u72ec\u5f53\u4e00\u9762\u800c\u5e7f\u53d7\u4f7f\u7528\u8005\u7684\u597d\u8bc4\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;\">\u4e4b\u6240\u4ee5\u53eb awk \u662f\u56e0\u4e3a\u5176\u53d6\u4e86\u4e09\u4f4d\u521b\u59cb\u4eba Alfred Aho\uff0cPeter Weinberger, \u548c Brian Kernighan \u7684 Family Name \u7684\u9996\u5b57\u7b26\u3002<\/span><span style=\"text-align: left;letter-spacing: 0.034em;\"><\/span><\/p>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u751f\u4fe1\u9886\u57df\uff0c\u6570\u636e\u683c\u5f0f\u590d\u6742\u591a\u6837\uff0c\u5bf9\u5176\u6570\u636e\u6587\u672c\u7684\u5904\u7406\u4ea7\u751f\u4e86\u65b0\u7684\u6311\u6218\u3002awk\u867d\u7136\u4e5f\u80fd\u505a\uff0c\u4f46\u662f\u7528\u8d77\u6765\u7ec8\u7a76\u8fd8\u662f\u6ca1\u6709\u90a3\u4e48\u5f97\u5fc3\u5e94\u624b\u3002\u5e7f\u5927\u751f\u4fe1\u4eba\u5c31\u60f3\uff0c\u8981\u662f\u80fd\u6709\u4e2a\u4e13\u95e8\u5904\u7406\u751f\u4fe1\u6570\u636e\u6587\u4ef6\u7684awk\u8be5\u6709\u591a\u597d\u554a~<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u751f\u4fe1\u9886\u57df\u7684\u5927\u725b\u674e\u6052\uff0c\u6293\u4f4f\u4e86\u8fd9\u4e00\u75db\u70b9\uff0c\u52a8\u624b\u5bf9awk\u7684\u6e90\u7801\u8fdb\u884c\u4e86\u6539\u9020\u3002\u4e13\u95e8\u9488\u5bf9\u751f\u4fe1\u9886\u57df\u7684\u6587\u672c\u5904\u7406\u5de5\u5177\u2014\u2014bioawk\u5c31\u7531\u6b64\u5e94\u8fd0\u800c\u751f\u4e86\u3002\u800c\u4e14\u662f\u9752\u51fa\u4e8e\u84dd\u800c\u80dc\u4e8e\u84dd\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5728\u505a\u751f\u4fe1\u7684\u4f60\u8fd8\u6ca1\u7528\u8fc7\u7684\u8bdd\uff0c\u8d76\u7d27\u6765\u8ddf\u5c0f\u679c\u4e0a\u624b\u5427~<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<h2 style=\"text-align: center;\"><em><span style=\"font-size: 20px;\"><strong><span style=\"font-family: \u9ed1\u4f53;\">\u8f6f\u4ef6\u5b89\u88c5<\/span><\/strong><\/span><\/em><strong><span style=\"mso-spacerun:'yes';font-family:\u9ed1\u4f53;mso-ascii-font-family:Arial;mso-hansi-font-family:Arial;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:16.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h2>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5c0f\u679c\u63a8\u8350\u7528conda\u5b89\u88c5<\/span><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<h1 style=\"text-align: center;\"><em style=\"letter-spacing: 0.034em;\"><\/em><\/h1>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\">conda install -y bioawk<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">\u5f53\u7136\u4e5f\u53ef\u4e0b\u8f7d\u6e90\u7801\u7f16\u8bd1<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"> sudo apt-get install byacc git <span class=\"code-snippet__comment\">#\u5b89\u88c5git<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> git <span class=\"code-snippet__built_in\">clone<\/span> git:\/\/github.com\/lh3\/bioawk.git <span class=\"code-snippet__comment\">#\u9ed8\u8ba4\u5b89\u88c5\u5728\u5f53\u524d\u76ee\u5f55<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> git <span class=\"code-snippet__built_in\">clone<\/span> git:\/\/github.com\/lh3\/bioawk.git <span class=\"code-snippet__variable\">${path}<\/span>\/bioawk <span class=\"code-snippet__comment\">#\u82e5\u52a0path\u5219\u5fc5\u987b\u4e3a\u7a7a\uff0c\u56e0\u6b64\u53ef\u5728\u60f3\u8981\u5b89\u88c5\u7684\u76ee\u5f55\u4e0b\u8d77\u4e00\u4e2a\u65b0\u540d\u79f0\uff0cgit\u4f1aclone\u5230\u5176\u4e0b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__built_in\">cd<\/span> bioawk<\/span><\/code><code><span class=\"code-snippet_outer\"> make<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__built_in\">echo<\/span> <span class=\"code-snippet__string\">\"export PATH=<span class=\"code-snippet__variable\">${path}<\/span>\/bioawk\/:${PATH}\"<\/span> >> ~\/.bashrc <\/span><\/code><code><span class=\"code-snippet_outer\"> . ~\/.bashrc <\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" data-type=\"jpeg\" data-w=\"1080\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_IlmBAWe69EMUPMhNEgrv6NXX85lJxg-22.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwyrj7kbUR3ULG9ZUbLrLkgibzL8qUpQROfe8ebc3gNSnu1dIlmBAWe69EMUPMhNEgrv6NXX85lJxg\/640?wx_fmt=jpeg'\" data-recalc-dims=\"1\" \/><\/p>\n<h2 style=\"text-align: center;\"><span style=\"font-size: 20px;\"><em><strong><span style=\"font-family: \u9ed1\u4f53;\"><span style=\"font-family: Arial;\">Bioawk<\/span>\u7684\u4f7f\u7528<\/span><\/strong><\/em><\/span><strong><span style=\"mso-spacerun:'yes';font-family:\u9ed1\u4f53;mso-ascii-font-family:Arial;mso-hansi-font-family:Arial;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:16.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h2>\n<h3><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>\u4e00\u3001\u5e2e\u52a9\u6587\u6863\u4e0e\u4f7f\u7528\u65b9\u6cd5<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/h3>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u9996\u5148\u6211\u4eec\u67e5\u770b\u4e00\u4e0b\u5e2e\u52a9\u6587\u6863\uff0c<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\">#\u67e5\u770b\u5e2e\u52a9\u6587\u6863<\/span><\/code><code><span class=\"code-snippet_outer\">bioawk -c help<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.16931982633863965\" data-s=\"300,640\" data-type=\"png\" data-w=\"691\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_NGCPdq2wHgmq6HwLKgHiaH0b7N0xxA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOictcnajbxgHBT1Sjf0q4eBa7fOrNGCPdq2wHgmq6HwLKgHiaH0b7N0xxA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code><span class=\"code-snippet_outer\">#bioawk\u7684\u9009\u9879\u53c2\u6570\uff0c\u4f7f\u7528\u65b9\u6cd5<\/span><\/code><code><span class=\"code-snippet_outer\">bioawk<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.044797687861271675\" data-s=\"300,640\" data-type=\"png\" data-w=\"692\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_WKRTytjqBIAHzktVKq3MUEKxUTpTrg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOicKMGEx17icecyfvrF8sCJk16FPWKRTytjqBIAHzktVKq3MUEKxUTpTrg\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u63a5\u4e0b\u91cc\u662f\u70b9\u56fe#\u70b9\u56fe<\/span><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">-F: \u6307\u5b9a\u5217\u4e0e\u5217\u4e4b\u95f4\u7684\u5206\u9694\u7b26\uff1b<\/span><\/code><code><span class=\"code-snippet_outer\">-c: \u652f\u6301\u7684\u6587\u4ef6\u683c\u5f0f\uff1b<\/span><\/code><code><span class=\"code-snippet_outer\">-v: \u8bbe\u7f6e\u81ea\u5b9a\u4e49\u7684\u53d8\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">-f: \u4ece\u6307\u5b9a\u7684\u6587\u4ef6\u4e2d\u8bfb\u53d6\u7a0b\u5e8f<\/span><\/code><code><span class=\"code-snippet_outer\">[file]: \u6700\u540e\u6307\u5b9a\u7684\u64cd\u4f5c\u6587\u4ef6\u3002<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\">\u63a5\u4e0b\u6765\u5c0f\u679c\u4f1a\u5e26\u5927\u5bb6\u7531\u6d45\u81f3\u6df1\u5b66\u4e60<span style=\"font-family:Calibri;\">bioawk<\/span>\u7684\u4f7f\u7528\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: \u5b8b\u4f53;\"><br \/><\/span><\/p>\n<h3><span style=\"font-size: 14px;\"><strong><span style=\"font-family: \u5b8b\u4f53;\">\u4e8c\u3001\u5904\u7406<span style=\"font-family: Calibri;\">fasta<\/span>\u5e8f\u5217\u6587\u4ef6<\/span><\/strong><strong><span style=\"font-family: Calibri;\"><o:p><\/o:p><\/span><\/strong><\/span><\/h3>\n<h4><span style=\"font-size: 14px;\"><span style=\"font-family: \u9ed1\u4f53;\">\uff08\u4e00\uff09<\/span><span style=\"font-family: Arial;\"><span style=\"font-family: \u9ed1\u4f53;\">\u6253\u5370<\/span>fasta<span style=\"font-family: \u9ed1\u4f53;\">\u5e8f\u5217<\/span>ID<span style=\"font-family: \u9ed1\u4f53;\">\u3001<\/span><\/span><span style=\"font-family: \u9ed1\u4f53;\">\u5e8f\u5217<\/span><span style=\"font-family: Arial;\"><span style=\"font-family: \u9ed1\u4f53;\">\u957f\u5ea6\u3001<\/span>GC<span style=\"font-family: \u9ed1\u4f53;\">\u542b\u91cf<\/span><\/span><\/span><strong><span style=\"mso-spacerun:'yes';font-family:Arial;mso-fareast-font-family:\u9ed1\u4f53;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:14.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h4>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"makefile\"><code><span class=\"code-snippet_outer\">bioawk -cfastx '{print\" ID:\"$name\" t length:\"length($seq)\" t GC:\"gc($seq)\" \"}' xxx.fna<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">-c fastx: \u6307\u5b9a\u6587\u4ef6\u4e3afastx\u683c\u5f0f\uff0c\u5305\u62ecfasta\uff0cfastq<\/span><\/code><code><span class=\"code-snippet_outer\">\u2018{code}\u2019: \u4ee3\u7801\u9700\u8981\u7528{}\u62ec\u8d77\u6765<\/span><\/code><code><span class=\"code-snippet_outer\">$name: \u53d6\u6587\u4ef6\u4e2d\u7684\u53d8\u91cfname<\/span><\/code><code><span class=\"code-snippet_outer\">$seq: \u53d6\u6587\u4ef6\u4e2d\u7684\u5e8f\u5217<\/span><\/code><code><span class=\"code-snippet_outer\">length(): length\u51fd\u6570\u7edf\u8ba1\u5e8f\u5217\u957f\u5ea6<\/span><\/code><code><span class=\"code-snippet_outer\">gc(): \u5e8f\u5217\u4e2d\u7684gc\u542b\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">XXX.fna: \u6307\u5b9a\u7684\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">t: \u4e24\u5217\u4fe1\u606f\u7528\u5236\u8868\u7b26t\u5206\u5272<\/span><\/code><code><span class=\"code-snippet_outer\">\u2018 \u2019: \u811a\u672c{}\u88ab\u89c6\u4e3a\u5355\u4e2a\u6587\u672c\u5b57\u7b26\u4e32\u9700\u8981\u2019\u2019\u62ec\u8d77\u6765\uff0c\u5e76\u4e14\u5355\u5f15\u53f7\u8868\u793a\u8f93\u5165\u7684\u8d77\u59cb\u4e0e\u7ed3\u675f\u3002<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.1199421965317919\" data-s=\"300,640\" data-type=\"png\" data-w=\"692\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_QS8iazLoRWxhibia63ySVH2bPaoowA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOicfMABqdQYzpCRAV7k2iapR3B8yc3HQS8iazLoRWxhibia63ySVH2bPaoowA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\">\u8f93\u5165\u4ee3\u7801\u5373\u53ef\u6253\u5370\u7ed3\u679c\u5230\u7ec8\u7aef\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><br \/><\/o:p><\/span><\/p>\n<h4><span style=\"font-size: 14px;\"><strong><span style=\"font-family: \u9ed1\u4f53;\">\uff08<\/span><\/strong><strong><span style=\"font-family: \u9ed1\u4f53;\">\u4e8c<\/span><\/strong><strong><span style=\"font-family: \u9ed1\u4f53;\">\uff09<\/span><\/strong><strong><span style=\"font-family: \u9ed1\u4f53;\">\u83b7\u53d6<\/span><\/strong><strong><span style=\"font-family: Arial;\">fasta<\/span><\/strong><strong><span style=\"font-family: \u9ed1\u4f53;\">\u5e8f\u5217\u7684\u53cd\u5411\u4e92\u8865\u94fe\u5e8f\u5217<\/span><\/strong><\/span><\/h4>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8fd9\u662f\u793a\u4f8b\u6587\u4ef6demo.fasta,\u7684\u6570\u636e<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<h4><strong><span style=\"mso-spacerun:'yes';font-family:Arial;mso-fareast-font-family:\u9ed1\u4f53;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:14.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h4>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3\" data-s=\"300,640\" data-type=\"png\" data-w=\"200\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_UYaibiafliadey4JzSZP2urZXeWcVQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOicLr3gNDXPll0ibYxZ0zhyKFrCxNtDUYaibiafliadey4JzSZP2urZXeWcVQ\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\">#\u8f93\u51fa\u793a\u4f8b\u6570\u636e\u76845\u2019->3\u2019\u53cd\u5411\u4e92\u8865\u5e8f\u5217<\/span><\/code><code><span class=\"code-snippet_outer\">bioawk -c fastx '{ print \">\" $name;print revcomp($seq) }' demo.fasta > revdemo.fasta<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">\u4f7f\u7528revcomp()\u51fd\u6570\u5373\u53ef\u5f97\u52305\u2019->3\u2019\u53cd\u5411\u4e92\u8865\u5e8f\u5217<\/span><\/code><code><span class=\"code-snippet_outer\">revcomp($seq): \u62ec\u53f7\u4e2d\u8f93\u5165\u9700\u8981\u5904\u7406\u7684\u5e8f\u5217\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">> revdemo.fasta: \u5c06\u7ed3\u679c\u8f93\u51fa\u5230\u65b0\u7684fasta\u6587\u4ef6 revdemo\u4e2d<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3005464480874317\" data-s=\"300,640\" data-type=\"png\" data-w=\"183\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_JaOAwtIQ2LIBK7brz9egAic4sRhQGA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOicdYhIyKVrABabiab6gNicdfX3vaOJaOAwtIQ2LIBK7brz9egAic4sRhQGA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u4e0a\u8ff0\u7684\u5355\u56fe\u662f\u4e0d\u662f\u90fd\u5f88\u719f\u6089\uff0c\u90fd\u662f\u5e73\u65f6\u6211\u4eec\u505a\u7684\u6bd4\u8f83\u591a\u7684\u56fe\uff0c\u6211\u4eec\u521b\u5efa\u5b8c\u6210\u540e\uff0c\u5c31\u5f00\u59cb\u7ed8\u5236\u7ec4\u5408\u56fe\u7247\u628a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u8fd9\u91cc\u4f7f\u7528ggpubr\u5305\u4e2d\u51fd\u6570ggarrange\uff08\uff09\u5728\u4e00\u9875\u4e0a\u8fdb\u884c\u5c55\u793a\u4e0a\u8ff0\u7684\u7ed3\u679c<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u5bf9ToothGrowth\u6570\u636e\u96c6\u7684\u7bb1\u7ebf\u56fe\uff0c\u70b9\u56fe\u7ec4\u5408\u5c55\u793a\uff1a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">ggarrange(Box_plot, Dot_plot,labels = c(“A”, “B”),ncol = 2, nrow = 1)<\/span><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<h3><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><strong>\u4e09\u3001\u5904\u7406fastq\u6587\u4ef6<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/h3>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u6307\u5b9a\u7684\u6587\u4ef6\u683c\u5f0f\u4f9d\u7136\u9009\u62e9 -c fastx\uff0c\u5b83\u80fd\u591f\u81ea\u52a8\u8bc6\u522b\u5e8f\u5217\u662ffastq \u8fd8\u662f fasta\u3002Fastq\u6587\u4ef6\u6bd4fasta\u6587\u4ef6\u591a\u4e86\u6d4b\u5e8f\u8d28\u91cf\u7684\u4fe1\u606f\u3002<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u6211\u4eec\u7528\u4e8e\u6f14\u793a\u7684fastq\u90e8\u5206\u6587\u4ef6\u5982\u4e0b\uff1a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u662f\u4e0d\u662f\u5c31\u5b8c\u6210\u4e86\u5462\uff0cAB\u5e8f\u53f7\u5c0f\u4f19\u4f34\u53ef\u4ee5\u81ea\u884c\u8c03\u6574<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u540e\u9762\u6211\u4eec\u5bf9#mtcars \u6570\u636e\u96c6\u7684\u6761\u5f62\u56fe\uff0c\u6563\u70b9\u56fe\u7ec4\u5408\u5c55\u793a<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.2922173274596182\" data-s=\"300,640\" data-type=\"png\" data-w=\"681\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_AAtfDibNDzNslqzyzawSI3v0vIhicQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOictPmadtTwcyUgGwiblFxDs71AkqIAAtfDibNDzNslqzyzawSI3v0vIhicQ\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<h4><span style=\"font-size: 14px;\"><strong><span style=\"font-family: \u9ed1\u4f53;\">\uff08<\/span><\/strong><strong><span style=\"font-family: \u9ed1\u4f53;\">\u4e00<\/span><\/strong><strong><span style=\"font-family: \u9ed1\u4f53;\">\uff09<\/span><\/strong><strong><span style=\"font-family: Arial;\"><span style=\"font-family: \u9ed1\u4f53;\">\u8ba1\u7b97<\/span>fastq<span style=\"font-family: \u9ed1\u4f53;\">\u5e8f\u5217\u7684\u884c\u6570<\/span><\/span><\/strong><strong><span style=\"font-family: Arial;\"><o:p><\/o:p><\/span><\/strong><\/span><\/h4>\n<p><span style=\"font-family: \u5b8b\u4f53;font-size: 14px;\">\u6784\u5efa\u6d4b\u5e8f\u6570\u636e\u5206\u6790 <span style=\"font-family: Calibri;\">pipeline <\/span>\u65f6\uff0c\u5f53<\/span><span style=\"font-family: \u5b8b\u4f53;font-size: 14px;\">fastq<\/span><span style=\"font-family: \u5b8b\u4f53;font-size: 14px;\"><span style=\"font-family: Calibri;\"> <\/span>\u6570\u636e\u5728\u505a\u5b8c <span style=\"font-family: Calibri;\">trimming <\/span>\u540e\uff0c\u6211\u4eec\u5f80\u5f80\u8981\u5173\u6ce8\u5269\u4e0b\u591a\u5c11 <span style=\"font-family: Calibri;\">reads<\/span>\uff0c\u8fd9\u662f\u53ef\u4ee5\u7528<span style=\"font-family: Calibri;\"> <\/span><\/span><span style=\"font-family: \u5b8b\u4f53;font-size: 14px;\">bioawk<\/span><span style=\"font-family: \u5b8b\u4f53;font-size: 14px;\"><span style=\"font-family: Calibri;\"> <\/span>\u8fdb\u884c\u5feb\u901f\u7edf\u8ba1\u3002<\/span><\/p>\n<p><span style=\"font-family: \u5b8b\u4f53;font-size: 14px;\"><br \/><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.06791907514450866\" data-s=\"300,640\" data-type=\"png\" data-w=\"692\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_ATXN906XYxnelicdrSuIdTOwPqPiaA.png?w=640\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-30_bioawk\u4e00\u4e2a\u8d85\u597d\u7528\u751f\u4fe1\u6587\u672c\u5904\u7406\u8f6f\u4ef6\/9_ATXN906XYxnelicdrSuIdTOwPqPiaA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOic9iafaUtCHMvOica3EoOjT4yyU5A5ATXN906XYxnelicdrSuIdTOwPqPiaA\/640?wx_fmt=png''\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\">bioawk -c fastx 'END {print NR}' SRR1039509_2.fastq<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.06791907514450866\" data-s=\"300,640\" data-type=\"png\" data-w=\"692\" style=\"font-size: 16px;letter-spacing: 0.578px;text-align: center;height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_ATXN906XYxnelicdrSuIdTOwPqPiaA.png?w=640\" onerror=\"this.src='\u56fe\u7247\/\u751f\u4fe1\u679c_2023-09-30_bioawk\u4e00\u4e2a\u8d85\u597d\u7528\u751f\u4fe1\u6587\u672c\u5904\u7406\u8f6f\u4ef6\/9_ATXN906XYxnelicdrSuIdTOwPqPiaA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOic9iafaUtCHMvOica3EoOjT4yyU5A5ATXN906XYxnelicdrSuIdTOwPqPiaA\/640?wx_fmt=png''\" data-recalc-dims=\"1\" \/><\/p>\n<p><br \/><\/p>\n<p><br \/><\/p>\n<h4><strong><span style=\"mso-spacerun:'yes';font-family:\u9ed1\u4f53;mso-ascii-font-family:Arial;mso-hansi-font-family:Arial;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:14.0000pt;mso-font-kerning:1.0000pt;\">\uff08\u4e8c\uff09\u7edf<\/span><\/strong><strong><span style=\"mso-spacerun:'yes';font-family:Arial;mso-fareast-font-family:\u9ed1\u4f53;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:14.0000pt;mso-font-kerning:1.0000pt;\"><span style=\"font-family:\u9ed1\u4f53;\">\u8ba1\u7279\u6b8a\u78b1\u57fa\u5f00\u5934\u7684<\/span> reads <span style=\"font-family:\u9ed1\u4f53;\">\u6570<\/span><\/span><\/strong><strong><span style=\"mso-spacerun:'yes';font-family:Arial;mso-fareast-font-family:\u9ed1\u4f53;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:14.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h4>\n<p><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><span style=\"font-family:\u5b8b\u4f53;\">\u5728\u4e00\u4e9b\u7279\u6b8a\u573a\u5408\u91cc\uff0c\u9700\u8981\u5206\u6790<\/span> reads <span style=\"font-family:\u5b8b\u4f53;\">\uff0c\u7528<\/span><\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\">bioawk<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"> <span style=\"font-family:\u5b8b\u4f53;\">\u53ef\u4ee5\u5f88\u65b9\u4fbf\u5feb\u901f\u6765\u5b8c\u6210\u3002\u6bd4\u5982\u7edf\u8ba1\u7279\u6b8a\u78b1\u57fa\u5f00\u5934\u7684 <\/span>reads <span style=\"font-family:\u5b8b\u4f53;\">\u6570\u3002<\/span><o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"># <span style=\"font-family:\u5b8b\u4f53;\">\u7edf\u8ba1\u4ee5<\/span>GATTAC<span style=\"font-family:\u5b8b\u4f53;\">\u5f00\u5934\u7684<\/span>reads<span style=\"font-family:\u5b8b\u4f53;\">\u7684\u6570\u91cf<\/span><o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\">bioawk -c fastx ‘$seq~\/^GATTAC\/ {++n} END { print n }’ my_fastq.tar.gz<o:p><\/o:p><\/span><\/p>\n<p><span style=\"mso-spacerun:"yes";font-family:\u7b49\u7ebf;mso-bidi-font-family:"Times New Roman";font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"># \u7edf\u8ba1\u4ee5GATTAC\u5f00\u5934\u7684reads\u7684\u6570\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\">bioawk -c fastx '$seq~\/^GATTAC\/ {++n} END { print n }' my_fastq.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><br \/><\/p>\n<h4><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\uff08\u4e09\uff09<strong>\u67e5\u770b<\/strong><strong><span style=\"font-family: Arial;\">GC\u542b\u91cf\u5927\u4e8e60%\u7684reads<span style=\"font-family: \u9ed1\u4f53;\">\u7684\u8d28\u91cf\u60c5\u51b5<\/span><\/span><\/strong><\/span><\/h4>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><br \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">bioawk<\/span> -c fastx <span class=\"code-snippet__string\">'{if (gc(<span class=\"code-snippet__variable\">$seq<\/span>)>0.6) printf \"%sn%snn\", <span class=\"code-snippet__variable\">$seq<\/span>, <span class=\"code-snippet__variable\">$qual<\/span>}'<\/span> my_fastq.tar.gz<\/span><\/code><\/pre>\n<\/section>\n<p style=\"margin-bottom: 0px;font-size: 16px;letter-spacing: 0.578px;text-align: left;text-wrap: wrap;\"><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8fd9\u91cc\u4f7f\u7528\u4e86if (gc($seq)>0.6) \u6761\u4ef6\u5224\u65ad\u8bed\u53e5\uff0c\u6761\u4ef6\u662fgc\u542b\u91cf\u5927\u4e8e60%\u3002<o:p><\/o:p><\/span><\/p>\n<p style=\"margin-bottom: 0px;font-size: 16px;letter-spacing: 0.578px;text-align: left;text-wrap: wrap;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u6253\u5370printf\uff0c\u5e8f\u5217 $seq\u4ee5\u53ca\u6d4b\u5e8f\u8d28\u91cf$qual<\/span><\/p>\n<p style=\"text-align: center;\"><br \/><\/p>\n<h4><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><strong>\uff08\u56db\uff09\u8ba1\u7b97\u5e8f\u5217\u5e73\u5747\u8d28\u91cf\u5206\u6570<\/strong><strong><o:p><\/o:p><\/strong><\/span><\/h4>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">bioawk -c fastx ‘{print “>”$name; print meanqual($qual)}’ SRR1039509_2.fastq<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u6211\u4eec\u53ef\u4ee5\u8fd0\u884cbioawk\u8ba1\u7b97\u6bcf\u6761\u5e8f\u5217\u7684\u5e73\u5747\u8d28\u91cf\u5206\u6570,\u901a\u8fc7\u51fd\u6570meanqual()\u6765\u5b9e\u73b0\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.0833333333333333\" data-s=\"300,640\" data-type=\"png\" data-w=\"252\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_lE4JF4zyhqLAFTbcicKYF5U7NVdRHA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOicblOZTibO7bECvwtszcl9rnZRibzlE4JF4zyhqLAFTbcicKYF5U7NVdRHA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\">\u8fd4\u56de\u5e8f\u5217\u7684\u540d\u79f0\uff0c\u4ee5\u53ca\u8d28\u91cf\u5206\u6570\u3002<\/span><span style=\"mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<p><br \/><\/p>\n<h4><span style=\"font-size: 14px;\"><strong><span style=\"font-family: \u9ed1\u4f53;\"><span style=\"font-family: \u9ed1\u4f53;\">\uff08\u4e94\uff09\u5c06<\/span><span style=\"font-family: Arial;\">fastq<\/span><span style=\"font-family: \u9ed1\u4f53;\">\u683c\u5f0f\u8f6c\u6362\u4e3a<\/span><span style=\"font-family: Arial;\">fasta<\/span><span style=\"font-family: \u9ed1\u4f53;\">\u683c\u5f0f<\/span><\/span><\/strong><\/span><strong><span style=\"mso-spacerun:'yes';font-family:\u9ed1\u4f53;mso-ascii-font-family:Arial;mso-hansi-font-family:Arial;mso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:bold;font-size:14.0000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/strong><\/h4>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">bioawk<\/span> -c fastx <span class=\"code-snippet__string\">'{print \">\"<span class=\"code-snippet__variable\">$name<\/span>; print <span class=\"code-snippet__variable\">$seq<\/span>}'<\/span> SRR1039509_2.fastq<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3598265895953757\" data-s=\"300,640\" data-type=\"png\" data-w=\"692\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_tLSmQ0c4BHpClljchdxickuYtr4CkA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwr5QjX1ib6GxPIDUZukroOicmn4rgawE2OYouUibmIiapN2Ib6QtLSmQ0c4BHpClljchdxickuYtr4CkA\/640?wx_fmt=png'\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\"><\/span><\/p>\n<p style=\"line-height: normal;\"><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;\">\u901a\u8fc7\u8c03\u7528\u5e8f\u5217name\u53d8\u91cf\u5e76\u52a0\u4e0a>\uff0c\u7b2c\u4e8c\u884c\u6253\u5370seq\u53d8\u91cf\uff0c\u5c31\u5c06fastq\u6587\u4ef6\u8f6c\u6362\u4e3afastp\u6587\u4ef6\u4e86\u3002<\/span><\/p>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);font-size: 16px;\">\n<section>\n<section style=\"margin-bottom: 8px;font-size: 32px;color: rgb(0, 150, 136);\">\n<section style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);line-height: normal;\"><span style=\"font-size: 14px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;color: rgb(0, 0, 0);\">\u597d\u4e86\u5c0f\u679c\u4eca\u5929\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\u4e86\uff0c<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 14px;color: rgb(0, 0, 0);\">\u5c0f\u4f19\u4f34\u4eec\uff0c\u4eca\u5929\u6709\u6ca1\u6709\u5b66\u5230\u65b0\u77e5\u8bc6\u5462\uff0c\u60f3\u8981\u7ee7\u7eed\u4e86\u89e3R\u8bed\u8a00\u5185\u5bb9\u53ef\u4ee5\u6301\u7eed\u5173\u6ce8\u5c0f\u679c\u54e6\uff5e\u6216\u8005\u4e5f\u53ef\u4ee5\u5173\u6ce8\u6211\u4eec\u7684\u5b98\u7f51\u4e5f\u4f1a\u6301\u7eed\u66f4\u65b0\u7684\u54e6\uff5e<\/span><strong style=\"letter-spacing: 0.578px;font-size: var(--articleFontsize);\"><\/strong><\/section>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;caret-color: rgba(0, 0, 0, 0);\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"width: 520px;\">\n<section style=\"border-top: 1px solid rgb(0, 0, 0);width: 519.479px;float: left;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<section style=\"margin-top: -3px;width: 6px;height: 6px;border-radius: 100%;float: right;background-color: rgb(46, 172, 109);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: -1em;padding: 3px 10px;display: inline-block;vertical-align: top;background-color: rgb(255, 255, 255);color: rgb(0, 154, 68);\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" 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