{"id":59743,"date":"2024-06-17T08:43:12","date_gmt":"2024-06-17T00:43:12","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=59743"},"modified":"2024-06-17T08:43:12","modified_gmt":"2024-06-17T00:43:12","slug":"%e8%bf%98%e5%9c%a8%e7%94%bb%e5%b8%b8%e8%a7%84%e7%9a%84%e7%81%ab%e5%b1%b1%e5%9b%be%ef%bc%9f%e5%b0%8f%e6%9e%9c%e6%95%99%e4%bd%a0%e8%ae%a9%e4%bd%a0%e7%9a%84%e7%81%ab%e5%b1%b1%e5%9b%be%e6%b4%bb","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=59743","title":{"rendered":"\u8fd8\u5728\u753b\u5e38\u89c4\u7684\u706b\u5c71\u56fe\uff1f\u5c0f\u679c\u6559\u4f60\u8ba9\u4f60\u7684\u706b\u5c71\u56fe\u201c\u6d3b\u201d\u8fc7\u6765\uff01"},"content":{"rendered":"<p><html><br \/>\n<head><br \/>\n<title><\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media 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powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section>\n<section powered-by=\"xiumi.us\">\n<section>\n<section powered-by=\"xiumi.us\">\n<section><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 14px;letter-spacing: 0.034em;\">\u5c0f\u4f19\u4f34\u5bf9\u706b\u5c71\u56fe\u4ee5\u53ca\u8033\u6fe1\u76ee\u67d3\u4e86\uff0c\u60f3\u5fc5\u505a\u6bcf\u4e2a\u5dee\u5f02\u5206\u6790\u7684\u65f6\u5019\uff0c\u90fd\u5c11\u4e0d\u4e86\u5b83\uff0c\u5f53\u7136\u4e5f\u5c11\u4e0d\u5c11\u70ed\u56fe\u7684\u8868\u8fbe\uff0c\u4e0d\u8fc7\u4eca\u5929\u7684\u4e3b\u89d2\u662f\u706b\u5c71\u56fe\uff0c\u5c0f\u679c\u5e26\u5c0f\u4f19\u4f34\u505a\u4e0d\u4e00\u6837\u7684\u706b\u5c71\u56fe\uff0c\u8ba9\u5c0f\u4f19\u4f34\u53d1\u8868\u9ad8\u6c34\u5e73\u7684\u56fe\u7247\u3002<\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p><span style=\"text-align: left;letter-spacing: 0.034em;\"><\/span><\/p>\n<\/section>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 14px;\">\u706b\u5c71\u56fe\u7684\u542b\u4e49\u6211\u4eec\u4e5f\u4e0d\u8fc7\u591a\u7684\u4ecb\u7ecd\uff0c\u4e00\u822c\u7684\u6211\u4eec\u5e38\u7528\u6765\u5c55\u793a\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u7684\u5206\u5e03\uff0c\u6a2a\u5750\u6807\u4e00\u822c\u4e3aFold change\uff0c\u8d8a\u504f\u79bb\u4e2d\u5fc3\u5dee\u5f02\u500d\u6570\u5c31\u8d8a\u5927\uff1b\u6a2a\u5750\u6807\u5c31\u4e3aP value\u4e5f\u5c31\u662f\u6211\u4eec\u5e38\u89c1\u5230P\u503c\uff0c\u503c\u8d8a\u5927\u5462\u5c31\u8d8a\u663e\u8457\uff0c\u63a5\u4e0b\u6765\u5c31\u8ddf\u968f\u5c0f\u679c\u53bb\u5b66\u4e60\u5427!<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 14px;\">\u8fd9\u91cc\u6211\u4eec\u4f7f\u7528\u7684\u662f\u6211\u4eec\u7684\u8001\u670b\u53cbggplot2<\/span><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 14px;\"><em style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;letter-spacing: 0.578px;white-space: normal;font-size: 15px;color: rgb(178, 178, 178);\"><span style=\"font-size: 13px;text-decoration: underline;\">\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c111&#8243;,\u9886\u53d6\u4ee3\u7801\uff0c\u4ee3\u7801\u7f16\u53f7\uff1a231006<\/span><\/em><\/span><\/p>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<h1 style=\"text-align: center;\"><em style=\"letter-spacing: 0.034em;\"><\/em><\/h1>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"powershell\"><code><span class=\"code-snippet_outer\">library(ggplot2)<span class=\"code-snippet__comment\">#\u8c03\u7528\u4e00\u4e0b\uff0c\u987a\u4fbf\u5bfc\u5165\u6211\u4eec\u7684\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">data<\/span> &lt;- read.csv(<span class=\"code-snippet__string\">\"\u706b\u5c71data.csv\"<\/span>,header=TRUE,row.names = <span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">head(<span class=\"code-snippet__keyword\">data<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<section powered-by=\"xiumi.us\">\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.28715728715728717\" data-s=\"300,640\" data-type=\"png\" data-w=\"693\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_e9XdgdicB3cSib7PKo1VHDfP1pAteQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5UA1ZCqiay5PuTazSXd1cWgU5cFUe9XdgdicB3cSib7PKo1VHDfP1pAteQ\/640?wx_fmt=png'\"  data-recalc-dims=\"1\" \/><span style=\"background-color: rgb(250, 250, 250);color: rgb(51, 51, 51);font-family: Consolas, &quot;Liberation Mono&quot;, Menlo, Courier, monospace;font-size: 14px;letter-spacing: 0.034em;white-space: pre-wrap;text-align: justify;\"><\/span><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 14px;\">#\u67e5\u770b\u6570\u636e\u7c7b\u578b\uff0c\u4e3b\u8981\u6709P\u503c\uff0cFold change\u548c\u57fa\u56e0ID\u5373\u53ef\uff0c\u540e\u9762\u6709\u4e00\u4e2aLABEL\uff0c\u53ef\u80fd\u4f1a\u6709\u5c0f\u4f19\u4f34\u7591\u60d1\uff0c\u540e\u9762\u5c0f\u679c\u4f1a\u4ecb\u7ecd\u5230\uff0c\u4e0d\u8981\u62c5\u5fc3\uff0c\u5c0f\u4f19\u4f34\u81ea\u884c\u628a\u5176\u4ed6\u6570\u636e\u8bbe\u7f6e\u597d\u5c31\u53ef\u4ee5\u8ddf\u7740\u5c0f\u679c\u7ee7\u7eed\u5b66\u4e60<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 14px;\">\u5148\u7ed8\u5236\u7b80\u5355\u7684\u706b\u5c71\u56fe\u70b9\u56fe<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.0187590187590188\" data-s=\"300,640\" data-type=\"png\" data-w=\"693\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_sEAXibKcTIjvkTNicINZ1QsZpqHfWg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5rZvb1l4MML2D74Bj6yrQWX4icwzsEAXibKcTIjvkTNicINZ1QsZpqHfWg\/640?wx_fmt=png'\"  data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-size: 14px;font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">\u662f\u4e0d\u662f\u5355\u8c03\uff0c\u6ca1\u6709\u4efb\u4f55\u4fe1\u606f\u8ba9\u6211\u4eec\u53bb\u5206\u6790\uff0c\u6211\u4eec\u63a5\u4e0b\u6765\u628a\u7ec6\u8282\u53bb\u4f18\u5316\u4e00\u4e0b\u6bd4\u5982\uff1a\u6700\u91cd\u8981\u7684\u4e0a\u4e0b\u8c03\u57fa\u56e0\u7684\u533a\u5206\uff0c\u6a2a\u7eb5\u8f74\u7684\u9608\u503c\u7ebf\uff0c\u91cd\u70b9\u57fa\u56e0\u7684\u6807\u6ce8\uff0c\u6211\u4eec\u4e00\u6b65\u4e00\u6b65\u6765<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\"><span style=\"font-size: 14px;\">\u5148\u6839\u636e\u9608\u503c\u8bbe\u5b9a\u4e0a\u4e0b\u8c03\u7684\u57fa\u56e0<\/span> <span style=\"font-size: 14px;\">\u8fd9\u91cc\u6211\u4eec\u7528\u4ee3\u7801\u65b0\u589echange\u5217\uff0c\u7136\u540e\u5229\u7528ifelse\u51fd\u6570\u6dfb\u52a0\u57fa\u56e0\u7684\u4e0a\u4e0b\u8c03\u60c5\u51b5\uff0c\u7136\u540ecolor\u8fdb\u884c\u533a\u5206\uff0c\u6700\u540e\u4f7f\u7528geom<\/span>_hline<span style=\"font-size: 14px;\">\u548cg<\/span>eon_vline()\u7684\u53c2\u6570\u53bb\u8bbe\u7f6e\u9608\u503c\u7ebf\u3002<\/span><span style=\"mso-spacerun:&#39;yes&#39;;font-family:\u7b49\u7ebf;mso-bidi-font-family:&#39;Times New Roman&#39;;font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"objectivec\"><code><span class=\"code-snippet_outer\">data$change &lt;- as.factor(ifelse(data$adj.P.Val &lt; <span class=\"code-snippet__number\">0.01<\/span> &amp; abs(data$logFC) &gt; <span class=\"code-snippet__number\">1<\/span>,ifelse(data$logFC &gt; <span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__string\">'UP','DOWN'<\/span>),<span class=\"code-snippet__string\">'NOT'<\/span>))<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.1704545454545454\" data-s=\"300,640\" data-type=\"png\" data-w=\"440\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_xPzpdXSLboX7RhRsGnSzMHycf5qUOQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5ic0oNlOqwoowSb4okK1WqDEojxPzpdXSLboX7RhRsGnSzMHycf5qUOQ\/640?wx_fmt=png'\"  data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 10.5pt;\">\u662f\u4e0d\u662f\u589e\u52a0\u4e86\u4e00\u5217\u65b0\u7684<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 10.5pt;\">\u7136\u540e\u6dfb\u52a0\u9608\u503c\u7ebf\u8fd8\u6709\u989c\u8272\u533a\u522b<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9841269841269841\" data-s=\"300,640\" data-type=\"png\" data-w=\"693\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_DorONmueT4Zb2NibuhyMzQomxd7d9w.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5zGWbAESu7z3HiblhFKeIk8jTUXDorONmueT4Zb2NibuhyMzQomxd7d9w\/640?wx_fmt=png'\"  data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 10.5pt;\">\u8fd9\u6837\u4e00\u6765\u5c31\u660e\u663e\u591a\u4e86\uff0c\u5c0f\u4f19\u4f34\u5f80\u5f80\u7ed8\u5236\u5230\u8fd9\u91cc\u5c31\u4e0d\u5f80\u4e0b\u7ed8\u5236\u4e86\u8fd9\u6837\u4e00\u6765\u6211\u4eec\u663e\u8457\u7684\u57fa\u56e0\u4e5f\u6ca1\u529e\u6cd5\u5728\u56fe\u7247\u4e2d\u5bfb\u627e\u5230\uff0c\u6211\u4eec\u53bb\u6807\u6ce8\u91cd\u70b9\u663e\u8457\u7684\u5dee\u5f02\u57fa\u56e0\uff1a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">\u8fd9\u91cc\u6211\u4eec\u65b0\u589esign\u5217\uff0c\u5229\u7528ifelse\u51fd\u6570\u6dfb\u52a0\u91cd\u70b9\u663e\u8457\u5dee\u5f02\u57fa\u56e0\uff0c\u7136\u540e\u4f7f\u7528geom_text\u53c2\u6570\u6dfb<\/span><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 10.5pt;letter-spacing: 0.034em;\">\u52a0\u5230\u56fe\u4e0a<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.0078585461689586\" data-s=\"300,640\" data-type=\"png\" data-w=\"509\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_lN8pYGMf4mnUNloicMFIriaHfOx2wg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5dr3dJRSqv2QCpDcAQjq9Xpgicd0lN8pYGMf4mnUNloicMFIriaHfOx2wg\/640?wx_fmt=png'\"  data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 14px;\">\u7136\u540e\u7ed8\u5236\u56fe\u7247<\/span><span style=\"mso-spacerun:&#39;yes&#39;;font-family:\u7b49\u7ebf;mso-bidi-font-family:&#39;Times New Roman&#39;;font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code><span class=\"code-snippet_outer\">ggplot(data = data, aes(x = logFC, y = -log10(adj.P.Val), color = change)) +<\/span><\/code><code><span class=\"code-snippet_outer\">  geom_point(alpha=0.8, size = 1) +<\/span><\/code><code><span class=\"code-snippet_outer\">  theme_bw(base_size = 15) +<\/span><\/code><code><span class=\"code-snippet_outer\">  theme(panel.grid.minor = element_blank(),panel.grid.major = element_blank()) +<\/span><\/code><code><span class=\"code-snippet_outer\">  geom_hline(yintercept=2 ,linetype=4) +<\/span><\/code><code><span class=\"code-snippet_outer\">  geom_vline(xintercept=c(-1,1) ,linetype=4 ) +<\/span><\/code><code><span class=\"code-snippet_outer\">  scale_color_manual(name = \"\", values = c(\"red\", \"green\", \"black\"), limits = c(\"UP\", \"DOWN\", \"NOT\")) +<\/span><\/code><code><span class=\"code-snippet_outer\">  geom_text(aes(label = sign), size = 3)<\/span><\/code><\/pre>\n<\/section>\n<p><br  \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.0057720057720059\" data-s=\"300,640\" data-type=\"png\" data-w=\"693\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_tNNRwCAs6hZDqGLGce1T5ibKltV2sQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5sTtKsPMjjCvw3nv36DBWXBSRUtNNRwCAs6hZDqGLGce1T5ibKltV2sQ\/640?wx_fmt=png'\"  data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 10.5pt;\">\u7ed3\u679c\u5c31\u51fa\u6765\u4e86\uff0c\u4e0d\u8fc7\u5c0f\u4f19\u4f34\u53d1\u73b0\u5f88\u591a\u7684\u57fa\u56e0\u90fd\u662f\u91cd\u53e0\u7684\uff0c\u6ca1\u529e\u6cd5\u5206\u6e05\u695a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 10.5pt;\">\u8fd9\u4e2a\u65f6\u5019\u5c31\u7528\u5230ggplot2\u5305\u4e2d\u7684ggrepel\u6211\u4eec\u7684\u8001\u670b\u53cb\u5566<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">\u8fd9\u4e2a\u5305\u53ef\u4ee5\u5e2e\u6211\u4eec\u89e3\u51b3\u91cd\u53e0\u7684\u95ee\u9898\uff1a<\/span><span style=\"mso-spacerun:&#39;yes&#39;;font-family:\u7b49\u7ebf;mso-bidi-font-family:&#39;Times New Roman&#39;;font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code><span class=\"code-snippet_outer\">library(ggrepel)<\/span><\/code><code><span class=\"code-snippet_outer\">ggplot(data = data, aes(x = logFC, y = -log10(adj.P.Val), color = change)) +<\/span><\/code><code><span class=\"code-snippet_outer\">  geom_point(alpha=0.8, size = 1) +<\/span><\/code><code><span class=\"code-snippet_outer\">  theme_bw(base_size = 15) +<\/span><\/code><code><span class=\"code-snippet_outer\">  theme(panel.grid.minor = element_blank(),panel.grid.major = element_blank()) +<\/span><\/code><code><span class=\"code-snippet_outer\">  scale_color_manual(name = \"\", values = c(\"red\", \"green\", \"black\"), limits = c(\"UP\", \"DOWN\", \"NOT\")) +<\/span><\/code><code><span class=\"code-snippet_outer\">  geom_label_repel(aes(label=sign), fontface=\"bold\", color=\"grey50\", box.padding=unit(0.35, \"lines\"), point.padding=unit(0.5, \"lines\"), segment.colour = \"grey50\")<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9985569985569985\" data-s=\"300,640\" data-type=\"png\" data-w=\"693\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_F3nbT4TgI0UIgf7PKdPUMszH8ibozg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5knibcboKNWRHRURVSak5tUmrmXF3nbT4TgI0UIgf7PKdPUMszH8ibozg\/640?wx_fmt=png'\"  data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">\u662f\u4e0d\u662f\u660e\u663e\u4e0d\u4e00\u6837\u4e86 \u6bcf\u4e00\u4e2a\u57fa\u56e0\u90fd\u5f88\u6e05\u695a\u7684\u5c55\u793a\u51fa\u6765\uff0c<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">\u90a3\u5c0f\u4f19\u4f34\u60f3\u5c55\u793a\u81ea\u5df1\u60f3\u5c55\u793a\u7684\u57fa\u56e0\u600e\u4e48\u529e\uff0c\u4e0d\u8981\u56f0\u60d1\uff0c\u5c0f\u679c\u7ee7\u7eed\u6559\u5927\u5bb6\uff1a<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">\u6211\u4eec\u628a\u611f\u5174\u8da3\u7684\u57fa\u56e0\u6dfb\u52a0\u5728LABEL\u5217\uff0c\u5c31\u662f\u4e00\u5f00\u59cb\u5c0f\u679c\u5bfc\u5165\u6570\u636e\u7684\u5356\u7684\u5b98\u53f8\uff0c\u8fd9\u91cc\u5c0f\u679c\u81ea\u5df1\u968f\u4fbf\u5199\u4e86\u51e0\u4e2a\u57fa\u56e0\u7ed9\u5c0f\u4f19\u4f34\u6f14\u793a\u4e00\u4e0b\uff1a<\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.9262536873156342\" data-s=\"300,640\" data-type=\"png\" data-w=\"339\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_AHcvsTFIKgXV9GHbViaZB7FFJ6hIkg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5eHZkyFItDrWewC0UZf6nxG8V0AHcvsTFIKgXV9GHbViaZB7FFJ6hIkg\/640?wx_fmt=png'\"  data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-size: 10.5pt;font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">\u4e0b\u9762\u6211\u4eec\u53bb\u7ed8\u5236\u56fe\u7247\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code><span class=\"code-snippet_outer\">ggplot(data = data, aes(x = logFC, y = -log10(adj.P.Val), color = <span class=\"code-snippet__keyword\">change<\/span>)) +<\/span><\/code><code><span class=\"code-snippet_outer\">  geom_point(alpha=<span class=\"code-snippet__number\">0.8<\/span>, <span class=\"code-snippet__keyword\">size<\/span> = <span class=\"code-snippet__number\">1<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">  theme_bw(base_size = <span class=\"code-snippet__number\">15<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\">  theme(panel.grid.minor = element_blank(),panel.grid.major = element_blank() ) +<\/span><\/code><code><span class=\"code-snippet_outer\">  scale_color_manual(<span class=\"code-snippet__keyword\">name<\/span> = <span class=\"code-snippet__string\">\"\"<\/span>, <span class=\"code-snippet__keyword\">values<\/span> = c(<span class=\"code-snippet__string\">\"red\"<\/span>, <span class=\"code-snippet__string\">\"green\"<\/span>, <span class=\"code-snippet__string\">\"black\"<\/span>), limits = c(<span class=\"code-snippet__string\">\"UP\"<\/span>, <span class=\"code-snippet__string\">\"DOWN\"<\/span>, <span class=\"code-snippet__string\">\"NOT\"<\/span>)) +<\/span><\/code><code><span class=\"code-snippet_outer\">  geom_label_repel(aes(label=LABEL), fontface=<span class=\"code-snippet__string\">\"bold\"<\/span>, color=<span class=\"code-snippet__string\">\"grey50\"<\/span>, box.padding=unit(<span class=\"code-snippet__number\">0.35<\/span>, <span class=\"code-snippet__string\">\"lines\"<\/span>), point.padding=unit(<span class=\"code-snippet__number\">0.5<\/span>, <span class=\"code-snippet__string\">\"lines\"<\/span>), segment.colour = <span class=\"code-snippet__string\">\"grey50\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.0086580086580086\" data-s=\"300,640\" data-type=\"png\" data-w=\"693\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_ScjNOJFEGqNN7McoDR8Ihtj8YEl5aQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q51RgwEs3obpqSXPDaP3aWI0VBScjNOJFEGqNN7McoDR8Ihtj8YEl5aQ\/640?wx_fmt=png'\"  data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 10.5pt;\">\u662f\u4e0d\u662f\u5c31\u51fa\u73b0\u4e86\u5462\uff0c\u5c0f\u4f19\u4f34\u662f\u5426\u5b66\u4f1a\u5462\uff0c\u4e00\u5b9a\u8981\u4eb2\u624b\u53bb\u5b9e\u9a8c\u4e00\u4e0b\uff0c\u624d\u80fd\u5b66\u7684\u66f4\u7cbe\uff0c<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 10.5pt;\">\u4e0d\u8fc7\u5c0f\u679c\u5728\u8fd9\u91cc\u505a\u4e2a\u5c0f\u63d2\u66f2<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 10.5pt;\">\u6211\u4eec\u53ef\u4ee5\u5236\u4f5c\u4ea4\u4e92\u5f0f\u7684\u706b\u5c71\u56fe\uff0c\u867d\u7136\u4e0d\u80fd\u653e\u5728\u5c0f\u4f19\u4f34\u7684\u6587\u7ae0\u4e2d\uff0c\u4e0d\u8fc7\u5e73\u65f6\u6211\u4eec\u6c47\u62a5\u5176\u4ed6\u7528\u9014\u65f6\u5019\u8fd8\u662f\u5f88\u6709\u610f\u601d\u7684<o:p><\/o:p><\/span><\/p>\n<p><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 10.5pt;\">\u6765\u770b\u4e00\u4e0b\uff1a\u7528plot_ly\u51fd\u6570\u7ed8\u5236\u6563\u70b9\u56fe<\/span><span style=\"mso-spacerun:&#39;yes&#39;;font-family:\u7b49\u7ebf;mso-bidi-font-family:&#39;Times New Roman&#39;;font-size:10.5000pt;mso-font-kerning:1.0000pt;\"><o:p><\/o:p><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\">library(plotly)<\/span><\/code><code><span class=\"code-snippet_outer\">plot_ly(data,x = ~logFC, y = ~-log10(adj.P.Val),text = ~sign, type = 'scatter',  mode = 'markers')<\/span><\/code><\/pre>\n<\/section>\n<p><br  \/><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"1.115530303030303\" data-s=\"300,640\" data-type=\"png\" data-w=\"528\" style=\"height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_AXvvquKpzsDfHicdZZahnrXOnaicww.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyaPhVurqkEWmvKUnibibN3Q5RJJTXiagJPMMctYxdlY7pHWM5aBAXvvquKpzsDfHicdZZahnrXOnaicww\/640?wx_fmt=png'\"  data-recalc-dims=\"1\" \/><\/p>\n<p style=\"text-align: left;\"><span style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;font-size: 10.5pt;\">\u4f1a\u51fa\u73b0\u4e00\u4e2a\u9875\u9762\uff0c\u5f53\u6211\u4eec\u9f20\u6807\u653e\u4e0a\u53bb\u65f6\u5019\u5c31\u4f1a\u663e\u793a\u90a3\u4e2a\u70b9\u7684\u4fe1\u606f\uff0c\u662f\u4e0d\u662f\u5f88\u6709\u610f\u601d\uff0c<o:p><\/o:p><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 10.5pt;font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">\u4e0a\u8ff0\u201c\u6d3b\u201d\u8fc7\u6765\u7684\u706b\u5c71\u56fe\u662f\u5426\u5b66\u4f1a\u4e86\u5462\uff0c\u5c0f\u4f19\u4f34\u8981\u591a\u591a\u7406\u89e3\u4ee3\u7801\u7684\u610f\u601d\uff0c\u624d\u80fd\u7ed8\u5236\u51fa\u81ea\u5df1\u60f3\u8981\u7684\u56fe\u7247\uff01<\/span><strong style=\"color: rgb(121, 123, 170);font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;outline: 0px;font-size: 15px;letter-spacing: 0.544px;background-color: rgb(255, 255, 255);\"><\/strong><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;text-align: left;\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 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14px;\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/span><\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&amp;mid=2247491909&amp;idx=1&amp;sn=902e34bcf53e605fba2b457ec6a33e36&amp;chksm=cf7f7180f808f8961542f458a49da14e85d8831449fb65fbf2623d55affc564686a5422f56fd&amp;scene=21#wechat_redirect\" textvalue=\"3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\"><span style=\"font-size: 14px;\">3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8<\/span><\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&amp;mid=2247491905&amp;idx=1&amp;sn=fb34ebd6282962c7803badaee7a39096&amp;chksm=cf7f7184f808f8929608c0050344ad86193d0fc6455c1c18e16cc17bf4b37832b63d3b18284e&amp;scene=21#wechat_redirect\" textvalue=\"4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\"><span style=\"font-size: 14px;\">4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b<\/span><\/a><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: left;\"><span style=\"font-size: 10.5pt;font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\"><a target=\"_blank\" 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data-recalc-dims=\"1\" \/><\/span><\/a><\/span><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.3888888888888889\" data-s=\"300,640\" data-type=\"jpeg\" data-w=\"1080\" style=\"font-size: var(--articleFontsize);letter-spacing: 0.578px;text-align: center;height: auto !important;\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_SibFRUHIk0cCbShYgOsGDPrxeHkEtw.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAwyrj7kbUR3ULG9ZUbLrLkgvrEB1jg0GibYczZiaOORjzpxpIjSibFRUHIk0cCbShYgOsGDPrxeHkEtw\/640?wx_fmt=jpeg'\"  data-recalc-dims=\"1\" \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd8\u5728\u753b\u5e38\u89c4\u7684\u706b\u5c71\u56fe\uff1f\u5c0f\u679c\u6559\u4f60\u8ba9\u4f60\u7684\u706b\u5c71\u56fe\u201c\u6d3b\u201d\u8fc7\u6765\uff01 \u539f\u521b&nbsp;&nbsp;\u5c0f\u679c&nbsp;&nbsp; [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/59743"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=59743"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/59743\/revisions"}],"predecessor-version":[{"id":59756,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/59743\/revisions\/59756"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=59743"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=59743"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=59743"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}