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50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u683c\u5f0f\u4ecb\u7ecd<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>-c\u6307\u5b9a\u6587\u4ef6\u683c\u5f0f\uff1a\u5e38\u7528\u7684\u6709bed\u3001sam\u3001vcf\u3001gff\u3001fastax<\/p>\n<p>-c –help\uff0c\u67e5\u770b\u53ef\u4ee5\u7528\u7684\u683c\u5f0f\uff0c\u5728\u4f7f\u7528\u8fc7\u7a0b\u4e2d\u5982\u679c\u5bf9\u683c\u5f0f\u4e0d\u6e05\u695a\uff0c\u53ef\u4ee5\u53cd\u590d\u67e5\u770b\u786e\u5b9a\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">$ bioawk -<span class=\"code-snippet__built_in\">c<\/span> --help<\/span><\/code><code><span class=\"code-snippet_outer\">bed:<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__number\">1<\/span>:chrom <span class=\"code-snippet__number\">2<\/span>:start <span class=\"code-snippet__number\">3<\/span>:end <span class=\"code-snippet__number\">4<\/span>:name <span class=\"code-snippet__number\">5<\/span>:score <span class=\"code-snippet__number\">6<\/span>:strand <span class=\"code-snippet__number\">7<\/span>:thickstart <span class=\"code-snippet__number\">8<\/span>:thickend <span class=\"code-snippet__number\">9<\/span>:rgb <span class=\"code-snippet__number\">10<\/span>:blockcount <span class=\"code-snippet__number\">11<\/span>:blocksizes <span class=\"code-snippet__number\">12<\/span>:blockstarts <\/span><\/code><code><span class=\"code-snippet_outer\">sam:<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__number\">1<\/span>:qname <span class=\"code-snippet__number\">2<\/span>:flag <span class=\"code-snippet__number\">3<\/span>:rname <span class=\"code-snippet__number\">4<\/span>:pos <span class=\"code-snippet__number\">5<\/span>:mapq <span class=\"code-snippet__number\">6<\/span>:cigar <span class=\"code-snippet__number\">7<\/span>:rnext <span class=\"code-snippet__number\">8<\/span>:pnext <span class=\"code-snippet__number\">9<\/span>:tlen <span class=\"code-snippet__number\">10<\/span>:seq <span class=\"code-snippet__number\">11<\/span>:qual <\/span><\/code><code><span class=\"code-snippet_outer\">vcf:<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__number\">1<\/span>:chrom <span class=\"code-snippet__number\">2<\/span>:pos <span class=\"code-snippet__number\">3<\/span>:id <span class=\"code-snippet__number\">4<\/span>:ref <span class=\"code-snippet__number\">5<\/span>:alt <span class=\"code-snippet__number\">6<\/span>:qual <span class=\"code-snippet__number\">7<\/span>:filter <span class=\"code-snippet__number\">8<\/span>:info <\/span><\/code><code><span class=\"code-snippet_outer\">gff:<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__number\">1<\/span>:seqname <span class=\"code-snippet__number\">2<\/span>:source <span class=\"code-snippet__number\">3<\/span>:feature <span class=\"code-snippet__number\">4<\/span>:start <span class=\"code-snippet__number\">5<\/span>:end <span class=\"code-snippet__number\">6<\/span>:score <span class=\"code-snippet__number\">7<\/span>:filter <span class=\"code-snippet__number\">8<\/span>:strand <span class=\"code-snippet__number\">9<\/span>:group <span class=\"code-snippet__number\">10<\/span>:attribute <\/span><\/code><code><span class=\"code-snippet_outer\">fastx:<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__number\">1<\/span>:name <span class=\"code-snippet__number\">2<\/span>:seq <span class=\"code-snippet__number\">3<\/span>:qual <span class=\"code-snippet__number\">4<\/span>:comment<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.18055555555555555\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_CDGlh4eBWefkkBVVNo5LbPlyeNLHHQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7mDbP5XsumWLKXsrMIvDFAt0NCDGlh4eBWefkkBVVNo5LbPlyeNLHHQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u5b89\u88c5\u597d\u540e\uff0c\u5c31\u53ef\u4ee5\u7740\u624b\u4f7f\u7528\u4e86\uff0c\u5927\u6d77\u54e5\u603b\u7ed3\u4e86\u4e00\u4e9b\u5728\u751f\u4fe1\u5206\u6790\u8fc7\u7a0b\u4e2d\u53ef\u4ee5\u5e94\u7528\u7684<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p style=\"text-align: left;text-wrap: wrap;\"><strong>\u6848\u4f8b<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u4e0b\u9762\u5c31\u901a\u8fc7\u4e00\u4e2a\u4f8b\u5b50\u6765\u5c55\u793a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u9996\u5148\u51c6\u5907\u4e00\u4e2afastq\u683c\u5f0f\u7684\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">$ cat sample.fastq <\/span><\/code><code><span class=\"code-snippet_outer\">@A01403:<span class=\"code-snippet__number\">54<\/span>:HL3WFDRXY:<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">2101<\/span>:<span class=\"code-snippet__number\">8983<\/span>:<span class=\"code-snippet__number\">1204<\/span> <span class=\"code-snippet__number\">1<\/span>:N:<span class=\"code-snippet__number\">0<\/span>:AGACCGTA<\/span><\/code><code><span class=\"code-snippet_outer\">AACGGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAACTAGCCGTTGGGGTCCTTGAGACTTTAGTGGCGCAGCTAACGCATTAAGTTGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAAAGCGAAGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG<\/span><\/code><code><span class=\"code-snippet_outer\">+<\/span><\/code><code><span class=\"code-snippet_outer\">FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF,FFFFFFFFFFFF<\/span><\/code><code><span class=\"code-snippet_outer\">@A01403:<span class=\"code-snippet__number\">54<\/span>:HL3WFDRXY:<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">2101<\/span>:<span class=\"code-snippet__number\">16866<\/span>:<span class=\"code-snippet__number\">1485<\/span> <span class=\"code-snippet__number\">1<\/span>:N:<span class=\"code-snippet__number\">0<\/span>:AGACCGTA<\/span><\/code><code><span class=\"code-snippet_outer\">AACGGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCCAGCCGTTGGTGGGTTTACTCACCAGTGGCGCAGCTAACGCTTTAAGCATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGACGCAACGCGCAGAACCTTACCAGCCCTTGACATGTCCAGGACCGGTCGCAGAGATGTGACC<\/span><\/code><code><span class=\"code-snippet_outer\">+<\/span><\/code><code><span class=\"code-snippet_outer\">FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFF:FFFF:FFFFFFFFF:FFFF:<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.18796296296296297\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_q80sNUuiapYV6KPPHQ8XhvtAelILoA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7l9K1YP0RkjqGL97VbN9Jb7w7icq80sNUuiapYV6KPPHQ8XhvtAelILoA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"font-size: 18px;\">\u7edf\u8ba1\u5e8f\u5217\u7684\u957f\u5ea6<\/span><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#-c fastx \u6307\u793a\u51fa\u76f8\u5e94\u7684\u683c\u5f0f\uff0c\u6253\u5370\u51faname\u5217\u548cseq\u7684\u957f\u5ea6\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">$ bioawk -<span class=\"code-snippet__built_in\">c<\/span> fastx <span class=\"code-snippet__string\">'{print $name\"t\" length($seq)}'<\/span> sample.fastq<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.10555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_Jm5WZV30aEXiacvTjPQVL6CZmr6rlQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia743pDcVcoDp9CcFoelHDicyEaNUJm5WZV30aEXiacvTjPQVL6CZmr6rlQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u662f\u4e0d\u662f\u5f88\u65b9\u4fbf\uff0c\u63a5\u4e0b\u6765\u518d\u6765\u63a2\u7d22\u4ee5\u4e0b\u5176\u5b83\u7684\u529f\u80fd\u53ed\uff5e<\/p>\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">\u53d6\u7279\u5b9a\u957f\u5ea6bp\u7684\u5e8f\u5217<\/span><\/strong><\/p>\n<p>\u53d6\u51fa\u5927\u4e8e200bp\u5e8f\u5217\u7684id \uff0c\u5e8f\u5217\u7b49\u7b49<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">$ bioawk -<span class=\"code-snippet__built_in\">c<\/span> fastx <span class=\"code-snippet__string\">'length($seq)>200{print $name}'<\/span> sample.fastq<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.12407407407407407\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_MSS12Pj997nhMNIt2WCqSjibRl3Ywg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7uOwv5nfp0D9wRPYiazaakWI5L3MSS12Pj997nhMNIt2WCqSjibRl3Ywg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">\u5bf9\u5e8f\u5217\u6dfb\u52a0\u6807\u7b7e<\/span><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">$ bioawk -<span class=\"code-snippet__built_in\">c<\/span> fastx <span class=\"code-snippet__string\">'{print \">ASV\" $name; $seq}'<\/span> sample.fastq <\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.13333333333333333\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_w2tiar19n2wPtmWnqVouge6icjWZcg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia78tSldG1cUdKHknkBRxPkav5KfTw2tiar19n2wPtmWnqVouge6icjWZcg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">\u7edf\u8ba1GC\u542b\u91cf<\/span><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">$ bioawk -<span class=\"code-snippet__built_in\">c<\/span> fastx <span class=\"code-snippet__string\">'{print $name,gc($seq)}'<\/span> sample.fastq<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.13333333333333333\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_Y9OEicgQdWdYLw8l3UzlJdJzdI4TWQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7qJrakGpW6BiaiaIzkgWvFvMc910Y9OEicgQdWdYLw8l3UzlJdJzdI4TWQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">fastq\u8f6cfasta<\/span><\/strong><\/p>\n<p>\u6309bioawk\u7684\u539f\u7406\uff0c\u8fd9\u4e2a\u8f6c\u5316\u5c06\u53d8\u5f97\u975e\u5e38\u7684\u7b80\u5355\uff0c\u5c0f\u4f19\u4f34\u4eec\u53ef\u4ee5\u81ea\u5df1\u601d\u8003\u4e00\u4e0b\uff0c\u6216\u8005\u53ef\u4ee5\u7528\u4f60\u719f\u6089\u7684\u7f16\u7a0b\u8bed\u8a00\u5b9e\u73b0\u4e5f\u662f\u53ef\u4ee5\u7684\u54e6\uff5e<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">$ bioawk -<span class=\"code-snippet__built_in\">c<\/span> fastx <span class=\"code-snippet__string\">'{print \">\"$name;print $seq }'<\/span> sample.fastq<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.11018518518518519\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_aSvD0hWj6bLGEjbkhSvibsSbZUYw0Q.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7DQibPs7wVO1ia2IE80ndXKpjuXPiaSvD0hWj6bLGEjbkhSvibsSbZUYw0Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u662f\u4e0d\u662f\u5f88\u7b80\u5355\u7684\u4e00\u53e5\uff0c\u771f\u7684\u662f\u592a\u65b9\u4fbf\u4e86\uff0c\u518d\u63a5\u89e6\u8fd9\u4e2a\u8f6f\u4ef6\u4e4b\u524d\u5927\u6d77\u54e5\u4e5f\u5c1d\u8bd5\u7528\u5176\u5b83\u7684\u65b9\u5f0f\u8fdb\u884cfastq\u4e0efasta\u4e4b\u95f4\u7684\u8f6c\u5316\uff0c\u4f8b\u5982\uff1b<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">cat sample.fastq |paste - - - - |cut -f <span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">2<\/span>|sed <span class=\"code-snippet__string\">'s\/^@\/>\/'<\/span>|tr <span class=\"code-snippet__string\">\"t\"<\/span> <span class=\"code-snippet__string\">\"n\"<\/span>|awk <span class=\"code-snippet__string\">'{print $1}'<\/span>>gene.fa<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.09907407407407408\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_M4VU7ExKr9kiaZNoQ3Z7PEnTX7TTxw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7GnHtCcY8bg5VYA1mxA0EzSnQwM4VU7ExKr9kiaZNoQ3Z7PEnTX7TTxw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">\u6839\u636e\u5e8f\u5217id\u63d0\u53d6\u5e8f\u5217<\/span><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">bioawk -cfastx <span class=\"code-snippet__string\">'BEGIN{while((getline k <\"id.txt\")>0)i[k]=1}{if(i[$name])print \">\"$name\"n\"$seq}'<\/span> sample.fastq<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.06759259259259259\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_MscicS0pF0eUmWHQ3E4HphUb9MLskg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7GdhsibjPK5ncSgV9rKtlFTfKDCMscicS0pF0eUmWHQ3E4HphUb9MLskg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u8fd9\u91cc\u7684id.txt\u5b58\u653e\u7684\u662f\u4e0d\u5e26\u201c>\u201d\u7684id<\/p>\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">\u8fc7\u6ee4\u6389\u77ed\u4e8e10bp\u7684reads<\/span><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">bioawk -<span class=\"code-snippet__built_in\">c<\/span> fastx <span class=\"code-snippet__string\">'length($seq) > 10 {print \"@\"$name\"n\"$seq\"n+n\"$qual}'<\/span> input.fastq<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">\u5904\u7406SAM\u6587\u4ef6<\/span><\/strong><\/p>\n<p>\u5c06\u672a\u6bd4\u5bf9\u4e0a\u7684\u5e8f\u5217\u63d0\u53d6\u51fa\u6765\uff1abioawk -c sam ‘and($flag,4)’ input.sam<\/p>\n<p>\u5c06\u6bd4\u5bf9\u4e0a\u7684\uff08mapped\uff09\u5e8f\u5217\u63d0\u53d6\u51fa\u6765\uff1abioawk -c sam -H ‘!and($flag,4)’ input.sam<\/p>\n<p>\u6839\u636esam\u6587\u4ef6\u521b\u5efafasta\uff1abioawk -c sam ‘{ s=$seq; if\uff08and($flag,16)\uff09{s=revcomp($seq)} print “>”$qname\u201cn\u201ds’ input.sam > out.fasta<\/p>\n<p>#\u8fd9\u91ccsam\u53ef\u80fd\u4f1a\u5b58\u5728\u53cd\u5411\u5e8f\u5217\uff0c\u5982\u679cflag=16\u4e3a\u53cd\u5411\u5e8f\u5217\uff0c\u9700\u8981\u5148\u7528revcomp\u8fdb\u884c\u53cd\u8f6c\u540e\u518d\u8d4b\u503c\u7ed9s\uff0c\u6700\u540e\u6253\u5370\u51fa\u540d\u5b57\u548c\u76f8\u5e94\u7684\u5e8f\u5217\u5373\u53ef\u3002<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p style=\"text-align: left;text-wrap: wrap;\"><strong>\u603b\u7ed3<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u6839\u636e\u4e0a\u8ff0\u7684\u5b9e\u8df5\u64cd\u4f5c\uff0c\u53ef\u4ee5\u76f4\u89c2\u7684\u611f\u53d7\u5230\u5c0f\u5c0f\u7684\u8f6f\u4ef6\uff0c\u4f46\u6db5\u76d6\u7684\u529f\u80fd\u662f\u975e\u5e38\u7684\u5f3a\u5927\u7684\u3002\u5728\u6570\u636e\u5904\u7406\u4e2d\u4e0d\u514d\u4f1a\u9047\u5230\u5404\u79cd\u5404\u6837\u7684\u95ee\u9898\uff0c\u6709\u65f6\u5019\u5199\u4ee3\u7801\u4f1a\u5f88\u7e41\u7410\u5e76\u4e14\u5bb9\u6613\u51fa\u73b0bug\u3002\u4e0d\u59a8\u5728\u5e73\u65f6\u591a\u591a\u79ef\u7d2f\u4e00\u4e9b\u597d\u7528\u7684\u751f\u4fe1\u8f6f\u4ef6\u534f\u52a9\u6211\u4eec\u89e3\u51b3\u95ee\u9898\u3002\u8fd9\u79cd\u5927\u4f6c\u5f00\u53d1\u7684\u4e13\u95e8\u7528\u4e8e\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u7684\u8f6f\u4ef6\uff0c\u4e0d\u4ec5\u80fd\u591f\u5feb\u901f\u7684\u4e86\u89e3\u751f\u7269\u4fe1\u606f\u5b66\u4e2d\u7684\u6587\u4ef6\u683c\u5f0f\uff0c\u5e76\u4e14\u4e0a\u624b\u7b80\u5355\uff0c\u975e\u5e38\u9002\u5408\u5c0f\u767d\u7684\u5b66\u4e60\u3002<\/p>\n<p><br \/><\/p>\n<p>\u597d\u4e86\uff0c\u8fd9\u6837\u6211\u4eec\u5c0f\u8f6f\u4ef6\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\u53ed\u3002\u5c0f\u4f19\u4f34\u4eec\u5982\u679c\u6709\u4ec0\u4e48\u95ee\u9898\u5c31\u548c\u5927\u6d77\u54e5\u8ba8\u8bba\u5427\u3002<\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;text-align: left;\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"width: 520px;\">\n<section style=\"margin-top: -3px;width: 6px;height: 6px;border-radius: 100%;float: right;background-color: rgb(46, 172, 109);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: -1em;padding: 3px 10px;display: inline-block;vertical-align: top;background-color: rgb(255, 255, 255);color: rgb(0, 154, 68);\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\"><span style=\"font-size: 14px;\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/span><\/a><br \/><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" 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{"id":59839,"date":"2024-06-17T08:49:25","date_gmt":"2024-06-17T00:49:25","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=59839"},"modified":"2024-06-17T08:49:25","modified_gmt":"2024-06-17T00:49:25","slug":"bioawk-%e7%a5%9e%e5%a5%87%e7%9a%84%e5%b0%8f%e8%bd%af%e4%bb%b6-3","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=59839","title":{"rendered":"bioawk\u2014\u2014\u795e\u5947\u7684\u5c0f\u8f6f\u4ef6"},"content":{"rendered":"

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powered-by=\"xiumi.us\">\n<p>\u751f\u4fe1\u4eba\u4e00\u5b9a\u7528\u8fc7\u4e09\u5251\u5ba2awk\u3001grep\u3001sed\uff0c\u751f\u4fe1\u725b\u4eba\u2014\u2014\u674e\u6052\uff0c\u6839\u636eawk\u7684\u6e90\u4ee3\u7801\uff0c\u8fdb\u884c\u4fee\u4fee\u8865\u8865\u4e13\u95e8\u7528\u4e8e\u751f\u4fe1\u7684\u5c0f\u5de5\u5177\u3002<\/p>\n<p><br \/><\/p>\n<p>\u4f5c\u8005\uff1a\u505a\u751f\u4fe1\u7684\u5e94\u8be5\u6ca1\u6709\u4eba\u4e0d\u77e5\u9053\u674e\u6052\u5927\u795e\u4e86\uff0c\u9f0e\u9f0e\u5927\u540d\u7684BWA\u57282009\u5e74\u52302019\u5e74\u77ed\u77ed10\u5e74\u7684\u5f15\u7528\u6b21\u6570\u5df2\u7ecf\u63a5\u8fd120K\u4e86\uff0c\u8fd9\u6837\u7684\u5f15\u7528\u6b21\u6570\u5bf9\u4e8e\u751f\u7269\u8f6f\u4ef6\u6765\u8bf4\u7edd\u5bf9\u662f\u6570\u4e00\u6570\u4e8c\u7684\u4e86\u3002\u674e\u6052\u7684\u6587\u7ae0\u968f\u968f\u4fbf\u4fbf\u5c31\u662f\u51e0\u5343\u7684\u5f15\u7528\u3002\u6700\u9ad8\u7684\u4e24\u7bc7\u5f15\u7528\u4e0a\u4e07\u6b21\u7684\uff0c\u5206\u522b\u662fBWA\u548cSAMtools\u5bf9\u5e94\u7684\u6587\u7ae0\u3002\u771f\u7684\u5f88\u611f\u8c22\u5927\u4f6c\u7ed9\u4e88\u6211\u4eec\u7684\u4fbf\u5229\uff0c\u5feb\u6765\u5b66\u4e00\u4e0b\u8fd9\u4e2a\u8f6f\u4ef6\u7684\u7b80\u4fbf\u529f\u80fd\u53ed\uff5e<\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" 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center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u5b89\u88c5bioawk<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u9996\u5148\uff0c\u6bd4bioawk\u662f\u5728Linux\u73af\u5883\u4e2d\u4f7f\u7528\u7684\uff0c\u63a8\u8350\u4f7f\u7528conda\u5b89\u88c5\u3002\u4e00\u884c\u547d\u4ee4\u5c31\u641e\u5b9a\uff0cconda\u7684\u4f7f\u7528\u8981\u7528-p\u6307\u5b9a\u5b89\u88c5\u76ee\u5f55\u3002<br \/> \u5b89\u88c5\u547d\u4ee4\uff1aconnda install -p ~\/conda path\/ bioawk -y<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p><strong>\u683c\u5f0f\u4ecb\u7ecd<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>-c\u6307\u5b9a\u6587\u4ef6\u683c\u5f0f\uff1a\u5e38\u7528\u7684\u6709bed\u3001sam\u3001vcf\u3001gff\u3001fastax<\/p>\n<p>-c –help\uff0c\u67e5\u770b\u53ef\u4ee5\u7528\u7684\u683c\u5f0f\uff0c\u5728\u4f7f\u7528\u8fc7\u7a0b\u4e2d\u5982\u679c\u5bf9\u683c\u5f0f\u4e0d\u6e05\u695a\uff0c\u53ef\u4ee5\u53cd\u590d\u67e5\u770b\u786e\u5b9a\u3002<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">$ bioawk -<span class=\"code-snippet__built_in\">c<\/span> --help<\/span><\/code><code><span class=\"code-snippet_outer\">bed:<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__number\">1<\/span>:chrom <span class=\"code-snippet__number\">2<\/span>:start <span class=\"code-snippet__number\">3<\/span>:end <span class=\"code-snippet__number\">4<\/span>:name <span class=\"code-snippet__number\">5<\/span>:score <span class=\"code-snippet__number\">6<\/span>:strand <span class=\"code-snippet__number\">7<\/span>:thickstart <span class=\"code-snippet__number\">8<\/span>:thickend <span class=\"code-snippet__number\">9<\/span>:rgb <span class=\"code-snippet__number\">10<\/span>:blockcount <span class=\"code-snippet__number\">11<\/span>:blocksizes <span class=\"code-snippet__number\">12<\/span>:blockstarts <\/span><\/code><code><span class=\"code-snippet_outer\">sam:<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__number\">1<\/span>:qname <span class=\"code-snippet__number\">2<\/span>:flag <span class=\"code-snippet__number\">3<\/span>:rname <span class=\"code-snippet__number\">4<\/span>:pos <span class=\"code-snippet__number\">5<\/span>:mapq <span class=\"code-snippet__number\">6<\/span>:cigar <span class=\"code-snippet__number\">7<\/span>:rnext <span class=\"code-snippet__number\">8<\/span>:pnext <span class=\"code-snippet__number\">9<\/span>:tlen <span class=\"code-snippet__number\">10<\/span>:seq <span class=\"code-snippet__number\">11<\/span>:qual <\/span><\/code><code><span class=\"code-snippet_outer\">vcf:<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__number\">1<\/span>:chrom <span class=\"code-snippet__number\">2<\/span>:pos <span class=\"code-snippet__number\">3<\/span>:id <span class=\"code-snippet__number\">4<\/span>:ref <span class=\"code-snippet__number\">5<\/span>:alt <span class=\"code-snippet__number\">6<\/span>:qual <span class=\"code-snippet__number\">7<\/span>:filter <span class=\"code-snippet__number\">8<\/span>:info <\/span><\/code><code><span class=\"code-snippet_outer\">gff:<\/span><\/code><code><span class=\"code-snippet_outer\"> <span class=\"code-snippet__number\">1<\/span>:seqname <span class=\"code-snippet__number\">2<\/span>:source <span class=\"code-snippet__number\">3<\/span>:feature <span class=\"code-snippet__number\">4<\/span>:start <span class=\"code-snippet__number\">5<\/span>:end <span class=\"code-snippet__number\">6<\/span>:score <span class=\"code-snippet__number\">7<\/span>:filter <span class=\"code-snippet__number\">8<\/span>:strand <span class=\"code-snippet__number\">9<\/span>:group <span class=\"code-snippet__number\">10<\/span>:attribute <\/span><\/code><code><span class=\"code-snippet_outer\">fastx:<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__number\">1<\/span>:name <span class=\"code-snippet__number\">2<\/span>:seq <span class=\"code-snippet__number\">3<\/span>:qual <span class=\"code-snippet__number\">4<\/span>:comment<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.18055555555555555\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_CDGlh4eBWefkkBVVNo5LbPlyeNLHHQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7mDbP5XsumWLKXsrMIvDFAt0NCDGlh4eBWefkkBVVNo5LbPlyeNLHHQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u5b89\u88c5\u597d\u540e\uff0c\u5c31\u53ef\u4ee5\u7740\u624b\u4f7f\u7528\u4e86\uff0c\u5927\u6d77\u54e5\u603b\u7ed3\u4e86\u4e00\u4e9b\u5728\u751f\u4fe1\u5206\u6790\u8fc7\u7a0b\u4e2d\u53ef\u4ee5\u5e94\u7528\u7684<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p style=\"text-align: left;text-wrap: wrap;\"><strong>\u6848\u4f8b<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u4e0b\u9762\u5c31\u901a\u8fc7\u4e00\u4e2a\u4f8b\u5b50\u6765\u5c55\u793a<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u9996\u5148\u51c6\u5907\u4e00\u4e2afastq\u683c\u5f0f\u7684\u6587\u4ef6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">$ cat sample.fastq <\/span><\/code><code><span class=\"code-snippet_outer\">@A01403:<span class=\"code-snippet__number\">54<\/span>:HL3WFDRXY:<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">2101<\/span>:<span class=\"code-snippet__number\">8983<\/span>:<span class=\"code-snippet__number\">1204<\/span> <span class=\"code-snippet__number\">1<\/span>:N:<span class=\"code-snippet__number\">0<\/span>:AGACCGTA<\/span><\/code><code><span class=\"code-snippet_outer\">AACGGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAACTAGCCGTTGGGGTCCTTGAGACTTTAGTGGCGCAGCTAACGCATTAAGTTGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAAAGCGAAGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG<\/span><\/code><code><span class=\"code-snippet_outer\">+<\/span><\/code><code><span class=\"code-snippet_outer\">FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF,FFFFFFFFFFFF<\/span><\/code><code><span class=\"code-snippet_outer\">@A01403:<span class=\"code-snippet__number\">54<\/span>:HL3WFDRXY:<span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">2101<\/span>:<span class=\"code-snippet__number\">16866<\/span>:<span class=\"code-snippet__number\">1485<\/span> <span class=\"code-snippet__number\">1<\/span>:N:<span class=\"code-snippet__number\">0<\/span>:AGACCGTA<\/span><\/code><code><span class=\"code-snippet_outer\">AACGGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCCAGCCGTTGGTGGGTTTACTCACCAGTGGCGCAGCTAACGCTTTAAGCATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGACGCAACGCGCAGAACCTTACCAGCCCTTGACATGTCCAGGACCGGTCGCAGAGATGTGACC<\/span><\/code><code><span class=\"code-snippet_outer\">+<\/span><\/code><code><span class=\"code-snippet_outer\">FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFF:FFFF:FFFFFFFFF:FFFF:<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.18796296296296297\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_q80sNUuiapYV6KPPHQ8XhvtAelILoA.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7l9K1YP0RkjqGL97VbN9Jb7w7icq80sNUuiapYV6KPPHQ8XhvtAelILoA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><strong><span style=\"font-size: 18px;\">\u7edf\u8ba1\u5e8f\u5217\u7684\u957f\u5ea6<\/span><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#-c fastx \u6307\u793a\u51fa\u76f8\u5e94\u7684\u683c\u5f0f\uff0c\u6253\u5370\u51faname\u5217\u548cseq\u7684\u957f\u5ea6\u3002<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">$ bioawk -<span class=\"code-snippet__built_in\">c<\/span> fastx <span class=\"code-snippet__string\">'{print $name\"t\" length($seq)}'<\/span> sample.fastq<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.10555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_Jm5WZV30aEXiacvTjPQVL6CZmr6rlQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia743pDcVcoDp9CcFoelHDicyEaNUJm5WZV30aEXiacvTjPQVL6CZmr6rlQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u662f\u4e0d\u662f\u5f88\u65b9\u4fbf\uff0c\u63a5\u4e0b\u6765\u518d\u6765\u63a2\u7d22\u4ee5\u4e0b\u5176\u5b83\u7684\u529f\u80fd\u53ed\uff5e<\/p>\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">\u53d6\u7279\u5b9a\u957f\u5ea6bp\u7684\u5e8f\u5217<\/span><\/strong><\/p>\n<p>\u53d6\u51fa\u5927\u4e8e200bp\u5e8f\u5217\u7684id \uff0c\u5e8f\u5217\u7b49\u7b49<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">$ bioawk -<span class=\"code-snippet__built_in\">c<\/span> fastx <span class=\"code-snippet__string\">'length($seq)>200{print $name}'<\/span> sample.fastq<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.12407407407407407\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_MSS12Pj997nhMNIt2WCqSjibRl3Ywg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7uOwv5nfp0D9wRPYiazaakWI5L3MSS12Pj997nhMNIt2WCqSjibRl3Ywg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">\u5bf9\u5e8f\u5217\u6dfb\u52a0\u6807\u7b7e<\/span><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">$ bioawk -<span class=\"code-snippet__built_in\">c<\/span> fastx <span class=\"code-snippet__string\">'{print \">ASV\" $name; $seq}'<\/span> sample.fastq <\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.13333333333333333\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_w2tiar19n2wPtmWnqVouge6icjWZcg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia78tSldG1cUdKHknkBRxPkav5KfTw2tiar19n2wPtmWnqVouge6icjWZcg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">\u7edf\u8ba1GC\u542b\u91cf<\/span><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">$ bioawk -<span class=\"code-snippet__built_in\">c<\/span> fastx <span class=\"code-snippet__string\">'{print $name,gc($seq)}'<\/span> sample.fastq<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.13333333333333333\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_Y9OEicgQdWdYLw8l3UzlJdJzdI4TWQ.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7qJrakGpW6BiaiaIzkgWvFvMc910Y9OEicgQdWdYLw8l3UzlJdJzdI4TWQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">fastq\u8f6cfasta<\/span><\/strong><\/p>\n<p>\u6309bioawk\u7684\u539f\u7406\uff0c\u8fd9\u4e2a\u8f6c\u5316\u5c06\u53d8\u5f97\u975e\u5e38\u7684\u7b80\u5355\uff0c\u5c0f\u4f19\u4f34\u4eec\u53ef\u4ee5\u81ea\u5df1\u601d\u8003\u4e00\u4e0b\uff0c\u6216\u8005\u53ef\u4ee5\u7528\u4f60\u719f\u6089\u7684\u7f16\u7a0b\u8bed\u8a00\u5b9e\u73b0\u4e5f\u662f\u53ef\u4ee5\u7684\u54e6\uff5e<\/p>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">$ bioawk -<span class=\"code-snippet__built_in\">c<\/span> fastx <span class=\"code-snippet__string\">'{print \">\"$name;print $seq }'<\/span> sample.fastq<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.11018518518518519\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_aSvD0hWj6bLGEjbkhSvibsSbZUYw0Q.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7DQibPs7wVO1ia2IE80ndXKpjuXPiaSvD0hWj6bLGEjbkhSvibsSbZUYw0Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u662f\u4e0d\u662f\u5f88\u7b80\u5355\u7684\u4e00\u53e5\uff0c\u771f\u7684\u662f\u592a\u65b9\u4fbf\u4e86\uff0c\u518d\u63a5\u89e6\u8fd9\u4e2a\u8f6f\u4ef6\u4e4b\u524d\u5927\u6d77\u54e5\u4e5f\u5c1d\u8bd5\u7528\u5176\u5b83\u7684\u65b9\u5f0f\u8fdb\u884cfastq\u4e0efasta\u4e4b\u95f4\u7684\u8f6c\u5316\uff0c\u4f8b\u5982\uff1b<\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">cat sample.fastq |paste - - - - |cut -f <span class=\"code-snippet__number\">1<\/span>,<span class=\"code-snippet__number\">2<\/span>|sed <span class=\"code-snippet__string\">'s\/^@\/>\/'<\/span>|tr <span class=\"code-snippet__string\">\"t\"<\/span> <span class=\"code-snippet__string\">\"n\"<\/span>|awk <span class=\"code-snippet__string\">'{print $1}'<\/span>>gene.fa<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.09907407407407408\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_M4VU7ExKr9kiaZNoQ3Z7PEnTX7TTxw.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7GnHtCcY8bg5VYA1mxA0EzSnQwM4VU7ExKr9kiaZNoQ3Z7PEnTX7TTxw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">\u6839\u636e\u5e8f\u5217id\u63d0\u53d6\u5e8f\u5217<\/span><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">bioawk -cfastx <span class=\"code-snippet__string\">'BEGIN{while((getline k <\"id.txt\")>0)i[k]=1}{if(i[$name])print \">\"$name\"n\"$seq}'<\/span> sample.fastq<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.06759259259259259\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_MscicS0pF0eUmWHQ3E4HphUb9MLskg.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/mmbiz_png\/YHGRXkSxjSRMgMJuBh6S6ZzD3H3PVcia7GdhsibjPK5ncSgV9rKtlFTfKDCMscicS0pF0eUmWHQ3E4HphUb9MLskg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"vertical-align: middle;width: 100%;\" data-recalc-dims=\"1\" \/><\/section>\n<\/section>\n<section style=\"font-size: 12px;line-height: 0;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u8fd9\u91cc\u7684id.txt\u5b58\u653e\u7684\u662f\u4e0d\u5e26\u201c>\u201d\u7684id<\/p>\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">\u8fc7\u6ee4\u6389\u77ed\u4e8e10bp\u7684reads<\/span><\/strong><\/p>\n<\/section>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"r\"><code><span class=\"code-snippet_outer\">bioawk -<span class=\"code-snippet__built_in\">c<\/span> fastx <span class=\"code-snippet__string\">'length($seq) > 10 {print \"@\"$name\"n\"$seq\"n+n\"$qual}'<\/span> input.fastq<\/span><\/code><\/pre>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p><br \/><\/p>\n<p><strong><span style=\"font-size: 18px;\">\u5904\u7406SAM\u6587\u4ef6<\/span><\/strong><\/p>\n<p>\u5c06\u672a\u6bd4\u5bf9\u4e0a\u7684\u5e8f\u5217\u63d0\u53d6\u51fa\u6765\uff1abioawk -c sam ‘and($flag,4)’ input.sam<\/p>\n<p>\u5c06\u6bd4\u5bf9\u4e0a\u7684\uff08mapped\uff09\u5e8f\u5217\u63d0\u53d6\u51fa\u6765\uff1abioawk -c sam -H ‘!and($flag,4)’ input.sam<\/p>\n<p>\u6839\u636esam\u6587\u4ef6\u521b\u5efafasta\uff1abioawk -c sam ‘{ s=$seq; if\uff08and($flag,16)\uff09{s=revcomp($seq)} print “>”$qname\u201cn\u201ds’ input.sam > out.fasta<\/p>\n<p>#\u8fd9\u91ccsam\u53ef\u80fd\u4f1a\u5b58\u5728\u53cd\u5411\u5e8f\u5217\uff0c\u5982\u679cflag=16\u4e3a\u53cd\u5411\u5e8f\u5217\uff0c\u9700\u8981\u5148\u7528revcomp\u8fdb\u884c\u53cd\u8f6c\u540e\u518d\u8d4b\u503c\u7ed9s\uff0c\u6700\u540e\u6253\u5370\u51fa\u540d\u5b57\u548c\u76f8\u5e94\u7684\u5e8f\u5217\u5373\u53ef\u3002<\/p>\n<p><br \/><\/p>\n<\/section>\n<section style=\"margin: 10px 0%;display: flex;flex-flow: row;text-align: center;justify-content: center;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: auto;flex: 0 0 0%;height: auto;line-height: 0;margin-right: -23px;z-index: 2;\">\n<section style=\"\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 16px;height: 12px;vertical-align: top;overflow: hidden;background-color: rgb(231, 215, 173);padding-right: 2px;padding-left: 2px;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"margin-top: 4px;margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 3px;height: 3px;vertical-align: top;overflow: hidden;border-width: 0px;border-radius: 155px;border-style: none;border-color: rgb(62, 62, 62);background-color: rgb(245, 245, 245);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"transform: translate3d(3px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 0px;height: 0px;vertical-align: top;overflow: hidden;border-style: solid;border-width: 0px 0px 4px 4px;border-color: rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgba(255, 255, 255, 0) rgb(231, 215, 173);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: top;width: auto;background-color: rgb(245, 241, 224);flex: 0 0 auto;align-self: flex-start;margin-top: 6px;min-width: 10%;height: auto;\">\n<section style=\"margin: 8px 0% 3px;\" powered-by=\"xiumi.us\">\n<section style=\"color: rgb(59, 149, 115);font-size: 17px;padding-right: 15px;padding-left: 15px;letter-spacing: 0px;text-align: justify;\">\n<p style=\"text-align: left;text-wrap: wrap;\"><strong>\u603b\u7ed3<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"font-size: 14px;line-height: 1.8;letter-spacing: 2px;padding-right: 10px;padding-left: 10px;\" powered-by=\"xiumi.us\">\n<p>\u6839\u636e\u4e0a\u8ff0\u7684\u5b9e\u8df5\u64cd\u4f5c\uff0c\u53ef\u4ee5\u76f4\u89c2\u7684\u611f\u53d7\u5230\u5c0f\u5c0f\u7684\u8f6f\u4ef6\uff0c\u4f46\u6db5\u76d6\u7684\u529f\u80fd\u662f\u975e\u5e38\u7684\u5f3a\u5927\u7684\u3002\u5728\u6570\u636e\u5904\u7406\u4e2d\u4e0d\u514d\u4f1a\u9047\u5230\u5404\u79cd\u5404\u6837\u7684\u95ee\u9898\uff0c\u6709\u65f6\u5019\u5199\u4ee3\u7801\u4f1a\u5f88\u7e41\u7410\u5e76\u4e14\u5bb9\u6613\u51fa\u73b0bug\u3002\u4e0d\u59a8\u5728\u5e73\u65f6\u591a\u591a\u79ef\u7d2f\u4e00\u4e9b\u597d\u7528\u7684\u751f\u4fe1\u8f6f\u4ef6\u534f\u52a9\u6211\u4eec\u89e3\u51b3\u95ee\u9898\u3002\u8fd9\u79cd\u5927\u4f6c\u5f00\u53d1\u7684\u4e13\u95e8\u7528\u4e8e\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u7684\u8f6f\u4ef6\uff0c\u4e0d\u4ec5\u80fd\u591f\u5feb\u901f\u7684\u4e86\u89e3\u751f\u7269\u4fe1\u606f\u5b66\u4e2d\u7684\u6587\u4ef6\u683c\u5f0f\uff0c\u5e76\u4e14\u4e0a\u624b\u7b80\u5355\uff0c\u975e\u5e38\u9002\u5408\u5c0f\u767d\u7684\u5b66\u4e60\u3002<\/p>\n<p><br \/><\/p>\n<p>\u597d\u4e86\uff0c\u8fd9\u6837\u6211\u4eec\u5c0f\u8f6f\u4ef6\u7684\u5206\u4eab\u5c31\u5230\u8fd9\u91cc\u53ed\u3002\u5c0f\u4f19\u4f34\u4eec\u5982\u679c\u6709\u4ec0\u4e48\u95ee\u9898\u5c31\u548c\u5927\u6d77\u54e5\u8ba8\u8bba\u5427\u3002<\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;text-align: left;\">\n<section style=\"font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;\">\n<section style=\"display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"width: 520px;\">\n<section style=\"margin-top: -3px;width: 6px;height: 6px;border-radius: 100%;float: right;background-color: rgb(46, 172, 109);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: -1em;padding: 3px 10px;display: inline-block;vertical-align: top;background-color: rgb(255, 255, 255);color: rgb(0, 154, 68);\">\n<p><strong>\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"text-align: justify;\">\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\"><span style=\"font-size: 14px;\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/span><\/a><br \/><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\"><span style=\"font-size: 14px;\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/span><\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491909&idx=1&sn=902e34bcf53e605fba2b457ec6a33e36&chksm=cf7f7180f808f8961542f458a49da14e85d8831449fb65fbf2623d55affc564686a5422f56fd&scene=21#wechat_redirect\" textvalue=\"3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\"><span style=\"font-size: 14px;\">3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8<\/span><\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491905&idx=1&sn=fb34ebd6282962c7803badaee7a39096&chksm=cf7f7184f808f8929608c0050344ad86193d0fc6455c1c18e16cc17bf4b37832b63d3b18284e&scene=21#wechat_redirect\" textvalue=\"4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\"><span style=\"font-size: 14px;\">4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b<\/span><\/a><\/section>\n<section style=\"letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493633&idx=1&sn=2263dfe7572c0bcf6816c2c1a37d79de&chksm=cf7f78c4f808f1d2209321d6ace39fde5fa60c0691b56f88eac21916f5a2872ce8ee345f1d5f&scene=21#wechat_redirect\" textvalue=\"\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" style=\"font-size: 14px;\" rel=\"noopener\"><span style=\"font-size: 14px;\">5.\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01<\/span><\/a><br \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;text-align: left;\"><span style=\"font-size: 10.5pt;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493633&idx=1&sn=2263dfe7572c0bcf6816c2c1a37d79de&chksm=cf7f78c4f808f1d2209321d6ace39fde5fa60c0691b56f88eac21916f5a2872ce8ee345f1d5f&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.4255555555555556\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_3enDMf2LD6pjia3zQolKXIfTwuh6AA-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxweqRKIWbRUBSWSGID9aog3q3QVgsHZplb1JyboXh8CPz6b3enDMf2LD6pjia3zQolKXIfTwuh6AA\/640?wx_fmt=jpeg&wxfrom=5&wx_lazy=1&wx_co=1'\" data-w=\"900\" data-recalc-dims=\"1\" \/><\/span><\/a><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_IWicymEetCc6Enf2BkUE4Yz1Ya5e5w.png?w=640\" 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