{"id":60027,"date":"2024-06-17T10:41:55","date_gmt":"2024-06-17T02:41:55","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=60027"},"modified":"2024-06-17T10:41:55","modified_gmt":"2024-06-17T02:41:55","slug":"%e5%bc%ba%e5%a4%a7%e7%9a%84%e5%9f%ba%e5%9b%a0%e7%ae%97%e6%b3%95%e5%b7%a5%e5%85%b7%e5%a4%a7%e5%b8%88%ef%bc%9abedtools-3","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=60027","title":{"rendered":"\u5f3a\u5927\u7684\u57fa\u56e0\u7b97\u6cd5\u5de5\u5177\u5927\u5e08\uff1abedtools"},"content":{"rendered":"


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solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar 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title=\"\u751f\u4fe1\u6709\u5173\u7edf\u8ba1\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u6709\u5173\u7edf\u8ba1<\/a><\/p>\n<\/div>\n<p style=\"text-align: center;\" data-mpa-powered-by=\"yiban.io\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.23333333333333334\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_rE2v0O5WiaUDJjgv5bxK2G1ePHyH2g-66.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAysHY49vPGCraibakiaNq6hQgn8C5MfvVLQGxlNZPEia2PajKAzrE2v0O5WiaUDJjgv5bxK2G1ePHyH2g\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-size: 15px;\">hello\uff0c\u4f60\u77e5\u9053\u6709\u4e00\u4e2a\u5f3a\u5927\u7684\u57fa\u56e0\u7ec4\u7b97\u6cd5\u5de5\u5177\u96c6\u561b\uff1f\u5b83\u53ef\u4ee5\u5e2e\u52a9\u6211\u4eec\u5904\u7406\u57fa\u56e0\u7ec4\u4e2d\u751f\u6210\u7684\u4e00\u7cfb\u5217bed\u3001bam\u7b49\u683c\u5f0f\u7684\u6587\u4ef6\uff0c\u60f3\u77e5\u9053\u5177\u4f53\u5982\u4f55\u64cd\u4f5c\u561b\uff1f\u611f\u5174\u8da3\u7684\u8bdd\u5c31\u548c\u5c0f\u679c\u4e00\u8d77\u770b\u4e0b\u53bb\u5427\uff01<\/span><\/p>\n<p><span style=\"font-size:21.0pt;mso-bidi-font-size:21.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;font-weight:bold;mso-bidi-font-weight:bold;\">\u4ec0\u4e48\u662fbedtools\uff1f<\/span><\/p>\n<p><span style=\"font-size: 15px;\">Bedtools \u662f\u4e00\u4e2a\u7528\u4e8e\u57fa\u56e0\u7ec4\u6570\u636e\u5206\u6790\u7684\u5f3a\u5927\u5de5\u5177\u96c6\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u751f\u7269\u4fe1\u606f\u5b66\u548c\u57fa\u56e0\u7ec4\u5b66\u9886\u57df\u3002\u5b83\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u7528\u4e8e\u5904\u7406 BED\uff08Browser Extensible Data\uff09\u683c\u5f0f\u6587\u4ef6\u7684\u547d\u4ee4\u884c\u5de5\u5177\uff0c\u53ef\u4ee5\u9ad8\u6548\u5730\u8fdb\u884c\u57fa\u56e0\u7ec4\u533a\u57df\u64cd\u4f5c\u3001\u91cd\u53e0\u5206\u6790\u3001\u6bd4\u8f83\u548c\u6ce8\u91ca\u7b49\u64cd\u4f5c\u3002<\/span><\/p>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_IWicymEetCc6Enf2BkUE4Yz1Ya5e5w-30.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyuYsqlJnQUJvnS2iciaDibtfbdV0VmHlriaSjYwjFPLp1BLjokHIWicymEetCc6Enf2BkUE4Yz1Ya5e5w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"letter-spacing: 0.578px;text-align: center;white-space: normal;\" data-recalc-dims=\"1\"><\/p>\n<p><span style=\"font-size: 15px;letter-spacing: 0.034em;\">Bedtools \u652f\u6301\u5404\u79cd\u5e38\u89c1\u7684\u57fa\u56e0\u7ec4\u64cd\u4f5c\uff0c\u5305\u62ec\u5e76\u96c6\u3001\u4ea4\u96c6\u3001\u5dee\u96c6\u3001\u5408\u5e76\u3001\u6392\u5e8f\u3001\u8fc7\u6ee4\u7b49\u3002\u901a\u8fc7\u8fd9\u4e9b\u64cd\u4f5c\uff0c\u7528\u6237\u53ef\u4ee5\u5c06\u4e0d\u540c\u7684\u57fa\u56e0\u7ec4\u533a\u57df\u6587\u4ef6\u8fdb\u884c\u7ec4\u5408\u3001\u7b5b\u9009\u548c\u6bd4\u8f83\uff0c\u4ece\u800c\u5b9e\u73b0\u5bf9\u57fa\u56e0\u7ec4\u533a\u57df\u7684\u8be6\u7ec6\u5206\u6790\u548c\u6ce8\u91ca\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">Bedtools \u8fd8\u53ef\u4ee5\u8fdb\u884c\u57fa\u56e0\u7ec4\u533a\u57df\u7684\u91cd\u53e0\u5206\u6790\u3002\u901a\u8fc7\u8ba1\u7b97\u4e24\u4e2a\u6216\u591a\u4e2a\u533a\u57df\u6587\u4ef6\u4e4b\u95f4\u7684\u91cd\u53e0\u60c5\u51b5\uff0c\u53ef\u4ee5\u786e\u5b9a\u57fa\u56e0\u6216\u5176\u4ed6\u529f\u80fd\u5143\u4ef6\u5728\u57fa\u56e0\u7ec4\u4e0a\u7684\u4f4d\u7f6e\u5173\u7cfb\uff0c\u5e76\u8fdb\u4e00\u6b65\u7814\u7a76\u5b83\u4eec\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u3002   <\/span><o:page><\/o:page><\/p>\n<p><span style=\"font-size: 15px;\">\u9664\u4e86\u57fa\u672c\u7684\u64cd\u4f5c\u548c\u91cd\u53e0\u5206\u6790\uff0cBedtools \u8fd8\u63d0\u4f9b\u4e86\u4e00\u4e9b\u9ad8\u7ea7\u529f\u80fd\uff0c\u4f8b\u5982\u57fa\u56e0\u7ec4\u533a\u57df\u7684\u7a81\u53d8\u5206\u6790\u3001\u7edf\u8ba1\u5206\u6790\u4ee5\u53ca\u57fa\u56e0\u7ec4\u529f\u80fd\u6ce8\u91ca\u7b49\u3002\u8fd9\u4f7f\u5f97 Bedtools \u6210\u4e3a\u4e86\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u5de5\u5177\u4e4b\u4e00\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u603b\u4e4b\uff0cBedtools \u662f\u4e00\u4e2a\u529f\u80fd\u5f3a\u5927\u7684\u57fa\u56e0\u7ec4\u6570\u636e\u5904\u7406\u5de5\u5177\u96c6\uff0c\u53ef\u4ee5\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u8fdb\u884c\u5404\u79cd\u57fa\u56e0\u7ec4\u533a\u57df\u7684\u64cd\u4f5c\u3001\u5206\u6790\u548c\u6ce8\u91ca\uff0c\u4e3a\u57fa\u56e0\u7ec4\u5b66\u548c\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\u63d0\u4f9b\u4e86\u91cd\u8981\u7684\u652f\u6301\u3002<\/span><\/p>\n<p><span style=\"font-size:21.0pt;mso-bidi-font-size:21.0pt;font-family:Open Sans;mso-ascii-font-family:Open Sans;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;font-weight:bold;mso-bidi-font-weight:bold;\">bedtools\u5904\u7406bed\u6587\u4ef6<\/span><\/p>\n<p><span style=\"font-size: 15px;\">bedtools\u5728\u5904\u7406\u4e00\u7cfb\u5217bed\u6587\u4ef6\u65f6\uff0c\u6839\u636e\u4e0d\u540c\u7684\u9700\u6c42\u6709\u4e0d\u540c\u7684\u547d\u4ee4\uff0c\u63a5\u4e0b\u6765\u5c0f\u679c\u5148\u4e3a\u5927\u5bb6\u4ecb\u7ecd\u4e94\u79cd\u6700\u5e38\u89c1\u7684\u64cd\u4f5c\u3002\u5728\u8fd9\u91cc\u6211\u4eec\u5148\u5f15\u5165\u4e09\u4e2a\u793a\u4f8b\u6587\u4ef6\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\">1.new-chr.bed<\/span><\/code><code><span class=\"code-snippet_outer\">2.newArea.bed<\/span><\/code><code><span class=\"code-snippet_outer\">3.newGene.bed<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-size: 15px;letter-spacing: 0.034em;\">\u4ee5\u62df\u5357\u82a5\u7684\u57fa\u56e0\u7ec4\u6570\u636e\u4e3a\u57fa\u7840\uff0cnew-chr.bed\u4ee3\u8868\u62df\u5357\u82a5\u57fa\u56e0\u7ec4\u4e0a7\u6761\u5e8f\u5217\u7684\u957f\u5ea6\u533a\u95f4\uff0cnewArea.bed\u4ee3\u8868\u6211<\/span><span style=\"font-size: 15px;letter-spacing: 0.034em;\">\u4eec\u901a\u8fc7\u6bd4\u5bf9\u8f6f\u4ef6\u6bd4\u5bf9\u540e\u4ea7\u751f\u7684\u533a\u95f4\u5408\u96c6\u6587\u4ef6\uff0cnewGene.bed\u4ee3\u8868\u6211\u4eec\u4ece\u62df\u5357\u82a5\u57fa\u56e0\u7ec4\u7684\u6ce8\u91ca<\/span><span style=\"font-size: 15px;letter-spacing: 0.034em;\">\u6587\u4ef6\u4e2d\u63d0\u53d6\u51fa\u7684\u57fa\u56e0\u4fe1\u606f\u533a\u95f4\u6587\u4ef6\u3002<\/span><span style=\"font-size: 15px;letter-spacing: 0.034em;\">\u8fd9\u91cc\u7684bed\u6587\u4ef6\u4ec5\u4f9b\u8bf4\u660e\u5de5\u5177\u7684\u529f\u80fd\uff0c\u5927\u5bb6\u5728\u5b66\u4e60\u65f6\u8981\u7075\u6d3b\u66ff\u6362\u6210\u81ea\u5df1\u7684\u6587\u4ef6\u54e6\uff01<\/span><br \/><\/p>\n<p><span style=\"font-size: 15px;\">\u5bf9\u6587\u4ef6\u6392\u5e8f    <\/span><o:page><\/o:page><\/p>\n<p><span style=\"font-size: 15px;\">\u5f53\u6211\u4eec\u62ff\u5230\u4e00\u4e2a\u533a\u95f4\u6587\u4ef6\u65f6\uff0c\u53ea\u6709\u5c06\u5176\u6392\u5e8f\u597d\u624d\u80fd\u4fdd\u8bc1\u540e\u7eed\u7684\u64cd\u4f5c\u66f4\u52a0\u51c6\u786e\uff0c\u6240\u4ee5\u6392\u5e8f\u8fd9\u4e00\u64cd\u4f5c\u5f80\u5f80\u662f\u5904\u7406bed\u6587\u4ef6\u7684\u7b2c\u4e00\u6b65\uff0c\u53ef\u4ee5\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\u6765\u8fdb\u884c\u6392\u5e8f\u64cd\u4f5c\uff1a<\/span><span style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\">bedtools sort -i newArea.bed > newSort.bed<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-size:12.0pt;mso-bidi-font-size:12.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;\">\u6392\u5e8f\u540e\uff0c\u4f60\u5c06\u5f97\u5230\u4e00\u4e2a\u6392\u5e8f\u597d\u7684\u533a\u95f4\u6587\u4ef6\uff1a<\/span><\/p>\n<p><span style=\"font-size:18.0pt;mso-bidi-font-size:18.0pt;font-family:Open Sans;mso-ascii-font-family:Open Sans;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;font-weight:bold;mso-bidi-font-weight:bold;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.9101307189542484\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_LSuPiaNj5VwPwBJ8ibhMh2bJHdAzhg.png?resize=408%2C371\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOuHDaMSCoDvAicN9ydGGI0nP25I8LSuPiaNj5VwPwBJ8ibhMh2bJHdAzhg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"612\" height=\"371 .27001953125\" width=\"408 .3399658203125\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p><span style=\"font-size:18.0pt;mso-bidi-font-size:18.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;font-weight:bold;mso-bidi-font-weight:bold;\">\u5408\u5e76\u76f8\u540c\u533a\u95f4<\/span><\/p>\n<p><span style=\"font-size:12.0pt;mso-bidi-font-size:12.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;\">\u5f53\u4f60\u6392\u5e8f\u5b8c\u6210\u540e\uff0c\u6709\u65f6\u6211\u4eec\u7684\u533a\u95f4\u4e2d\u6709\u5f88\u591a\u201c\u91cd\u590d\u201d\u7684\u533a\u95f4\uff0c\u5408\u5e76\u76f8\u540c\u4ee5\u53ca\u91cd\u53e0\u7684\u533a\u95f4\u53ef\u4ee5\u5e2e\u52a9\u7b80\u5316\u6211\u4eec\u7684\u533a\u95f4\uff0c\u8ba9\u6570\u636e\u66f4\u76f4\u89c2\u4ee5\u53ca\u7b80\u6d01\uff0c\u4f60\u53ef\u4ee5\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\u6765\u5b8c\u6210\u5408\u5e76\u64cd\u4f5c\uff1a<\/span><span style=\"white-space:pre-wrap;font-family: Consolas, "Liberation Mono", Menlo, Courier, monospace;font-size: 14px;background-color: rgb(250, 250, 250);color: rgb(51, 51, 51);letter-spacing: 0.034em;\">       <\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">bedtools<\/span> <span class=\"code-snippet__selector-tag\">merge<\/span> <span class=\"code-snippet__selector-tag\">-i<\/span> <span class=\"code-snippet__selector-tag\">newSort<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> > <span class=\"code-snippet__selector-tag\">newMerge<\/span><span class=\"code-snippet__selector-class\">.ned<\/span> <\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-size:12.0pt;mso-bidi-font-size:12.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;\">\u5408\u5e76\u540e\u7684\u6587\u4ef6\u683c\u5f0f\u5982\u4e0b\uff1a<\/span><\/p>\n<p><span style=\"font-size:18.0pt;mso-bidi-font-size:18.0pt;font-family:Open Sans;mso-ascii-font-family:Open Sans;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;font-weight:bold;mso-bidi-font-weight:bold;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"1.1666666666666667\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_lia9NfibnJIiaEuZhvsVNo7oA2odgw.png?resize=464%2C541\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOhviajEM1Yve04a6jic4zPZsC90yOvlia9NfibnJIiaEuZhvsVNo7oA2odgw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"696\" height=\"541 .4099731445312\" width=\"464 .2099609375\" data-recalc-dims=\"1\"><\/span><\/p>\n<p><span style=\"font-size:18.0pt;mso-bidi-font-size:18.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;font-weight:bold;mso-bidi-font-weight:bold;\">\u6c42\u533a\u95f4\u7684\u8986\u76d6\u5ea6<\/span><\/p>\n<p><span style=\"font-size:12.0pt;mso-bidi-font-size:12.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;\">\u5f53\u4f60\u60f3\u6c42B\u6587\u4ef6\u5728A\u6587\u4ef6\u4e0a\u7684\u533a\u95f4\u8986\u76d6\u5ea6\u65f6\uff0c\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">bedtools<\/span> <span class=\"code-snippet__selector-tag\">coverage<\/span> <span class=\"code-snippet__selector-tag\">-a<\/span> <span class=\"code-snippet__selector-tag\">A<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> <span class=\"code-snippet__selector-tag\">-b<\/span> <span class=\"code-snippet__selector-tag\">B<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> > <span class=\"code-snippet__selector-tag\">C<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">eg<\/span>: <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">bedtools<\/span> <span class=\"code-snippet__selector-tag\">coverage<\/span> <span class=\"code-snippet__selector-tag\">-a<\/span> <span class=\"code-snippet__selector-tag\">new-chr<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> <span class=\"code-snippet__selector-tag\">-b<\/span> <span class=\"code-snippet__selector-tag\">newMerge<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> > <span class=\"code-snippet__selector-tag\">newOnChr<\/span><span class=\"code-snippet__selector-class\">.bed<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-size:12.0pt;mso-bidi-font-size:12.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;\">\u5c0f\u679c\u6e29\u99a8\u63d0\u793a\uff1a\u5728\u8fd9\u91cc\uff0c-a\u548c-b\u540e\u5206\u522b\u8f93\u5165\u4e24\u4e2a\u533a\u95f4\u6587\u4ef6\uff0c\u5176\u4e2d-a\u540e\u7684\u6587\u4ef6\u53c2\u6570\u8981\u8bbe\u7f6e\u4e3a\u4e24\u4e2a\u533a\u95f4\u6587\u4ef6\u4e2d\u8303\u56f4\u5927\u7684\u6587\u4ef6\u54e6\u3002<\/span>    <o:page><\/o:page><\/p>\n<p><span style=\"font-size:12.0pt;mso-bidi-font-size:12.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;\">\u8fd0\u884c\u540e\u5f97\u5230\u7684\u7ed3\u679c\u5982\u4e0b\uff1a<\/span><\/p>\n<p><span style=\"font-size:18.0pt;mso-bidi-font-size:18.0pt;font-family:Open Sans;mso-ascii-font-family:Open Sans;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;font-weight:bold;mso-bidi-font-weight:bold;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.1935185185185185\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_bsv5FA6zyia5vW9O2gRkUp9P6GLM7A.png?resize=553%2C107\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOnBsFlC5kNYxaPeqfW0BpAZcjNbsv5FA6zyia5vW9O2gRkUp9P6GLM7A\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"107 .33000183105469\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p><span style=\"font-size:18.0pt;mso-bidi-font-size:18.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;font-weight:bold;mso-bidi-font-weight:bold;\">\u6c42\u4e24\u4e2a\u533a\u95f4\u7684\u4ea4\u96c6<\/span><\/p>\n<p><span style=\"font-size:12.0pt;mso-bidi-font-size:12.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;\">\u5f53\u8981\u6c42\u4e24\u4e2a\u533a\u95f4\u6587\u4ef6\u7684\u4ea4\u96c6\u533a\u95f4\u65f6\uff0c\u4f60\u53ef\u4ee5\u4f7f\u7528\u4ee5\u4e0b\u547d\u4ee4<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">bedtools<\/span> <span class=\"code-snippet__selector-tag\">intersect<\/span> <span class=\"code-snippet__selector-tag\">-a<\/span> <span class=\"code-snippet__selector-tag\">A<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> <span class=\"code-snippet__selector-tag\">-b<\/span> <span class=\"code-snippet__selector-tag\">B<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> > <span class=\"code-snippet__selector-tag\">C<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">eg<\/span>: <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">bedtools<\/span> <span class=\"code-snippet__selector-tag\">intersect<\/span> <span class=\"code-snippet__selector-tag\">-a<\/span> <span class=\"code-snippet__selector-tag\">newSort<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> <span class=\"code-snippet__selector-tag\">-b<\/span> <span class=\"code-snippet__selector-tag\">newGene<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> > <span class=\"code-snippet__selector-tag\">newAndGene<\/span><span class=\"code-snippet__selector-class\">.bed<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-size:12.0pt;mso-bidi-font-size:12.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;\">\u6700\u540e\u5f97\u5230\u7684\u7ed3\u679c\u5982\u4e0b\uff1a<\/span>    <o:page><\/o:page><\/p>\n<p><span style=\"font-family:\u7b49\u7ebf;mso-ascii-font-family:\u7b49\u7ebf;mso-fareast-font-family:\u7b49\u7ebf;font-variant:normal;text-transform:none;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"1.1790393013100438\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_az9HOicktxU9E0OSm3BVBvM7EknOTA-1.png?resize=458%2C540\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSO9ksGCD5PGeBIict8Znks4HeqJViaz9HOicktxU9E0OSm3BVBvM7EknOTA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"687\" height=\"540 .0399169921875\" width=\"458 .030029296875\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p><span style=\"font-size:18.0pt;mso-bidi-font-size:18.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;font-weight:bold;mso-bidi-font-weight:bold;\">\u6c42\u4e24\u4e2a\u533a\u95f4\u7684\u5dee\u96c6<\/span><\/p>\n<p><span style=\"font-size:12.0pt;mso-bidi-font-size:12.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;\">\u5f53\u8981\u6c42\u4e24\u4e2a\u533a\u95f4\u7684\u5dee\u96c6\u65f6\uff0c\u6211\u4eec\u9700\u8981\u7528\u5230\u4ee5\u4e0b\u547d\u4ee4\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">bedtools<\/span> <span class=\"code-snippet__selector-tag\">subtract<\/span> <span class=\"code-snippet__selector-tag\">-a<\/span> <span class=\"code-snippet__selector-tag\">A<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> <span class=\"code-snippet__selector-tag\">-b<\/span> <span class=\"code-snippet__selector-tag\">B<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> > <span class=\"code-snippet__selector-tag\">C<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> <\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">eg<\/span>\uff1a<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">bedtools<\/span> <span class=\"code-snippet__selector-tag\">subtract<\/span> <span class=\"code-snippet__selector-tag\">-a<\/span> <span class=\"code-snippet__selector-tag\">newSort<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> <span class=\"code-snippet__selector-tag\">-b<\/span> <span class=\"code-snippet__selector-tag\">newGene<\/span><span class=\"code-snippet__selector-class\">.bed<\/span> > <span class=\"code-snippet__selector-tag\">newNoGene<\/span><span class=\"code-snippet__selector-class\">.bed<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-size:12.0pt;mso-bidi-font-size:12.0pt;font-family:\u5b8b\u4f53;mso-ascii-font-family:Open Sans;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;\">\u6ce8\u610f\uff0c\u4ee5\u4e0a\u547d\u4ee4\u6c42\u5f97\u7684\u65f6A.bed\u6587\u4ef6\u4e2d\u53bb\u9664\u4e86B.bed\u6587\u4ef6\u4e2d\u7684\u533a\u95f4\u5269\u4f59\u7684\u533a\u95f4\u6587\u4ef6\uff0c\u793a\u4f8b\u6587\u4ef6\u8fd0\u884c\u540e\u7684\u7ed3\u679c\u5982\u4e0b\uff1a<\/span>    <o:page><\/o:page><\/p>\n<p><span style=\"font-size:12.0pt;mso-bidi-font-size:12.0pt;font-family:Open Sans;mso-ascii-font-family:Open Sans;mso-fareast-font-family:\u5b8b\u4f53;mso-bidi-font-family:Open Sans;font-variant:normal;text-transform:none;color:#333333;mso-font-kerning:0.0pt;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"1.2465753424657535\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_2iaFypduOqd2QA8Xk6UuAKCQGtPTTA-1.png?resize=438%2C546\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOT3GQKj2JiclX5DrjdTl2SP9Jmib2iaFypduOqd2QA8Xk6UuAKCQGtPTTA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"657\" height=\"546 .2100219726562\" width=\"438 .07000732421875\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u4ee5\u4e0a\u5c31\u662fbedtools\u6700\u5e38\u7528\u7684\u4e94\u4e2a\u529f\u80fd\uff0c\u8fd8\u6709\u5f88\u591a\u4e30\u5bcc\u7684\u529f\u80fd\u7b49\u5f85\u4f60\u7ee7\u7eed\u63a2\u7d22\uff0c\u600e\u4e48\u6837\uff0c\u662f\u4e0d\u662f\u5f88\u7b80\u5355\uff1f\u8d76\u5feb\u62ff\u8d77\u4f60\u7684\u57fa\u56e0\u7ec4\u6570\u636e\u7ec3\u8d77\u6765\u5427\uff01\u66f4\u591a\u5b66\u4e60\u5e72\u8d27\u8981\u7ee7\u7eed\u5173\u6ce8\u5c0f\u679c\u54e6\uff01<\/span><\/p>\n<section powered-by=\"xiumi.us\" 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data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;\" rel=\"noopener\"><span style=\"outline: 0px;font-size: 14px;\">4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b<\/span><\/a><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493633&idx=1&sn=2263dfe7572c0bcf6816c2c1a37d79de&chksm=cf7f78c4f808f1d2209321d6ace39fde5fa60c0691b56f88eac21916f5a2872ce8ee345f1d5f&scene=21#wechat_redirect\" textvalue=\"\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;letter-spacing: 0.578px;font-size: 14px;\" rel=\"noopener\">5.\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01<\/a><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"margin-top: 0pt;margin-bottom: 0pt;margin-left: 0pt;letter-spacing: 0.578px;text-indent: 0pt;white-space: normal;text-align: left;line-height: normal;font-size: 12pt;font-family: helvetica;color: rgb(0, 0, 0);break-after: auto;\"><span style=\"font-family: \u5b8b\u4f53;font-size: 12pt;text-indent: 0pt;color: rgba(0, 0, 0, 0.9);letter-spacing: 0.034em;\"><\/span><span style=\"font-size: 15px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_FbEg9fibwQicL8l2ExKOZZTmAHXabQ-16.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAx3HetBkB7aOGibFWKqibqIATyC4zFibzCgdrxFwRep6cDMLIbA0FbEg9fibwQicL8l2ExKOZZTmAHXabQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;caret-color: rgba(0, 0, 0, 0);\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5f3a\u5927\u7684\u57fa\u56e0\u7b97\u6cd5\u5de5\u5177\u5927\u5e08\uff1abedtools \u539f\u521b  \u5c0f\u679c  \u751f\u4fe1\u679c&nb […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/60027"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=60027"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/60027\/revisions"}],"predecessor-version":[{"id":60037,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/60027\/revisions\/60037"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=60027"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=60027"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=60027"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}