{"id":60105,"date":"2024-06-17T10:47:23","date_gmt":"2024-06-17T02:47:23","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=60105"},"modified":"2024-06-17T10:47:23","modified_gmt":"2024-06-17T02:47:23","slug":"qualimap%ef%bc%9a%e4%b8%80%e6%ac%be%e5%bc%ba%e5%a4%a7%e7%9a%84%e7%94%9f%e4%bf%a1%e7%bb%98%e5%9b%be%e5%b7%a5%e5%85%b7%ef%bc%8c%e5%8a%a9%e5%8a%9b%e4%bd%a0%e7%9a%84%e6%b5%8b%e5%ba%8f%e6%95%b0%e6%8d%ae","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=60105","title":{"rendered":"Qualimap\uff1a\u4e00\u6b3e\u5f3a\u5927\u7684\u751f\u4fe1\u7ed8\u56fe\u5de5\u5177\uff0c\u52a9\u529b\u4f60\u7684\u6d4b\u5e8f\u6570\u636e\u5206\u6790"},"content":{"rendered":"


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\n<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner,body{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media{padding:20px;overflow:hidden;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}@media screen and (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media_content{overflow:hidden;color:#333;font-size:17px;word-wrap:break-word;-webkit-hyphens:auto;-ms-hyphens:auto;hyphens:auto;text-align:justify;position:relative;z-index:0}.rich_media_content *{max-width:100%!important;box-sizing:border-box!important;-webkit-box-sizing:border-box!important;word-wrap:break-word!important}.rich_media_content p{clear:both;min-height:1em}.rich_media_content em{font-style:italic}.rich_media_content fieldset{min-width:0}.rich_media_content .list-paddingleft-1,.rich_media_content .list-paddingleft-2,.rich_media_content .list-paddingleft-3{padding-left:2.2em}.rich_media_content .list-paddingleft-1 .list-paddingleft-2,.rich_media_content .list-paddingleft-2 .list-paddingleft-2,.rich_media_content .list-paddingleft-3 .list-paddingleft-2{padding-left:30px}.rich_media_content .list-paddingleft-1{padding-left:1.2em}.rich_media_content .list-paddingleft-3{padding-left:3.2em}.rich_media_content .code-snippet,.rich_media_content .code-snippet__fix{max-width:1000%!important}.rich_media_content .code-snippet *,.rich_media_content .code-snippet__fix *{max-width:1000%!important}.rich_media_title{font-size:22px;line-height:1.4;margin-bottom:13px;padding-bottom:13px;border-bottom:1px solid #e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation 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0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"Qualimap\uff1a\u4e00\u6b3e\u5f3a\u5927\u7684\u751f\u4fe1\u7ed8\u56fe\u5de5\u5177\uff0c\u52a9\u529b\u4f60\u7684\u6d4b\u5e8f\u6570\u636e\u5206\u6790\",\"time\":\"2023-12-15 19:00:15\",\"timeStamp\":\"1702638015\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497890&idx=1&sn=b505c975be42a09da2ff008410678169&chksm=cf7f6867f808e1716151114bb4e9d24a53908498c238a29fa1eb9f9a3963764d89d8691a8116#rd\" target=\"_blank\" rel=\"noopener\">Qualimap\uff1a\u4e00\u6b3e\u5f3a\u5927\u7684\u751f\u4fe1\u7ed8\u56fe\u5de5\u5177\uff0c\u52a9\u529b\u4f60\u7684\u6d4b\u5e8f\u6570\u636e\u5206\u6790<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b  <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c  <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c  <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-12-15 19:00:15<\/em><\/div>\n<p><content><\/p>\n<div class=\"topic\">\n<p>\u6536\u5f55\u4e8e\u8bdd\u9898<\/p>\n<p><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3138206666342268940#wechat_redirect\" title=\"\u751f\u4fe1\u5c0f\u767d\u76f8\u5173\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5c0f\u767d\u76f8\u5173<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2821072943068839938#wechat_redirect\" title=\"\u751f\u4fe1\u7ed8\u56fe\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u7ed8\u56fe<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3142740974448934918#wechat_redirect\" title=\"\u79d1\u7814\u4f5c\u56fe\" target=\"_blank\" rel=\"noopener\">#\u79d1\u7814\u4f5c\u56fe<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2664545260517294084#wechat_redirect\" title=\"\u751f\u4fe1\u5b9e\u64cd\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5b9e\u64cd<\/a><\/p>\n<\/div>\n<p style=\"text-align: center;\" data-mpa-powered-by=\"yiban.io\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.23333333333333334\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_rE2v0O5WiaUDJjgv5bxK2G1ePHyH2g-67.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAysHY49vPGCraibakiaNq6hQgn8C5MfvVLQGxlNZPEia2PajKAzrE2v0O5WiaUDJjgv5bxK2G1ePHyH2g\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<h1><span style=\"font-size: 15px;\">\u5927\u5bb6\u597d\uff0c\u5c0f\u679c\u4eca\u5929\u548c\u5927\u5bb6\u5206\u4eab\u4e00\u6b3e\u751f\u4fe1\u7ed8\u56fe\u5de5\u5177\u2014\u2014Qualimap\u3002Qualimap\u662f\u4e00\u6b3e\u975e\u5e38\u5b9e\u7528\u7684\u751f\u7269\u4fe1\u606f\u5b66\u8f6f\u4ef6\uff0c\u5b83\u53ef\u4ee5\u5e2e\u52a9\u4f60\u5bf9\u57fa\u56e0\u7ec4\u3001\u8f6c\u5f55\u7ec4\u548c\u8868\u89c2\u9057\u4f20\u7ec4\u7684\u6d4b\u5e8f\u6570\u636e\u8fdb\u884c\u8d28\u91cf\u8bc4\u4f30\u548c\u5206\u6790\u3002<br \/><\/span><\/h1>\n<p><span style=\"font-size: 15px;\">Qualimap\u7684\u529f\u80fd\u4e3b\u8981\u5305\u62ec\uff1a\u5bf9\u6d4b\u5e8f\u6570\u636e\u7684BAM\u6587\u4ef6\u8fdb\u884c\u8986\u76d6\u5ea6\u3001\u91cd\u590d\u5ea6\u3001\u63d2\u5165\u7247\u6bb5\u957f\u5ea6\u7b49\u8fdb\u884c\u7edf\u8ba1\u548c\u53ef\u89c6\u5316\uff1b\u5bf9\u57fa\u56e0\u7ec4\u53d8\u5f02\u3001\u7ed3\u6784\u53d8\u5f02\u3001\u8868\u89c2\u9057\u4f20\u6807\u8bb0\u7b49VCF\u6587\u4ef6\u8fdb\u884c\u68c0\u6d4b\u548c\u6ce8\u91ca\uff1b\u5bf9RNA-seq\u6570\u636e\u8fdb\u884c\u57fa\u56e0\u8868\u8fbe\u91cf\u8ba1\u7b97\u3001\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u3001\u529f\u80fd\u5bcc\u96c6\u5206\u6790\u7b49\uff1b\u5bf9ChIP-seq\u6570\u636e\u8fdb\u884c\u8d28\u91cf\u63a7\u5236\uff0c\u68c0\u67e5\u5cf0\u503c\u7684\u5bcc\u96c6\u5ea6\u3001\u5206\u5e03\u3001\u91cd\u53e0\u7b49\u6307\u6807\uff1b\u5bf9\u591a\u6837\u672c\u7684\u6570\u636e\u8fdb\u884c\u6bd4\u8f83\u5206\u6790\uff0c\u7ed8\u5236PCA\u56fe\u3001\u70ed\u56fe\u3001\u7bb1\u7ebf\u56fe\u7b49\uff1b\u5bf9\u57fa\u56e0\u7ec4\u6ce8\u91ca\u6587\u4ef6\u8fdb\u884c\u89e3\u6790\uff0c\u63d0\u53d6\u57fa\u56e0\u7ec4\u7279\u5f81\u4fe1\u606f\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u8ddf\u7740\u5c0f\u679c\u4e00\u8d77\u6d4b\u8bd5\u4e00\u4e0b\u5427\uff01<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u5b98\u65b9\u4e0b\u8f7d\u7f51\u5740\uff1a<\/span><span style=\"font-size: 15px;\">http:\/\/qualimap.conesalab.org\/<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 15px;\">\u6216\u8005GitHub\u4e0a\u7684\u6e90\u4ee3\u7801<\/span><span style=\"font-size: 15px;\">https:\/\/github.com\/EagleGenomics-cookbooks\/QualiMap<\/span><span style=\"font-size: 15px;\">    <\/span><o:page><\/o:page><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.7638888888888888\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_OzUxoUJXakAHXzrX9uQ9Ko2eAlZDAw.png?resize=503%2C384\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOShLzQYZrN9WX15Q4qFLhJRZHOzUxoUJXakAHXzrX9uQ9Ko2eAlZDAw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"384 .80999755859375\" width=\"503 .8900146484375\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-size: 15px;\">\u89e3\u538b\u5e76\u5b89\u88c5R\u5305\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code><span class=\"code-snippet_outer\">unzip qualimap_v2.3.zip<\/span><\/code><code><span class=\"code-snippet_outer\">cd qualimap_v2.3\/<\/span><\/code><code><span class=\"code-snippet_outer\">Qualimap\u9700\u8981\u5b89\u88c5\u7248\u672c6\u6216\u66f4\u9ad8\u7248\u672c\u7684Java\u548c3.1\u6216\u66f4\u9ad8\u7248\u672c\u7684R\u73af\u5883\uff0c<\/span><\/code><code><span class=\"code-snippet_outer\">Qualimap\u9700\u8981\u4ee5\u4e0bR\u5305\uff1aoptparse\uff08\u53ef\u4eceCRAN\u83b7\u5f97\uff09\uff0cNOISeq\uff0cRepitools\uff0cRsamtools\uff0cGenomicFeatures\uff0crtracklayer\uff08\u53ef\u4eceBioconductor\u83b7\u5f97\uff09\u3002\u60a8\u53ef\u4ee5\u624b\u52a8\u5b89\u88c5\u8fd9\u4e9b\u5305\uff0c\u4e5f\u53ef\u4ee5\u901a\u8fc7\u6267\u884c\u5b89\u88c5\u6587\u4ef6\u5939\u4e2d\u627e\u5230\u7684\u811a\u672c\u6765\u5b89\u88c5\u5b83\u4eec\uff1a<\/span><\/code><code><span class=\"code-snippet_outer\">sudo Rscript $QUALIMAP_HOME\/scripts\/installDependencies.r<\/span><\/code><code><span class=\"code-snippet_outer\">\u6765\u770b\u4e00\u4e0bqualimap\u7684\u7528\u6cd5\u548c\u529f\u80fd\uff1a<\/span><\/code><code><span class=\"code-snippet_outer\">\u00b7bamqc\uff1a\u8bc4\u4f30NGS\u6d4b\u5e8f\u6570\u636e\u4e0e\u53c2\u8003\u57fa\u56e0\u7ec4\u7684\u6bd4\u5bf9\u60c5\u51b5\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">\u00b7rnaseq\uff1a\u8bc4\u4f30RNA-seq\u6d4b\u5e8f\u6570\u636e\u7684\u6bd4\u5bf9\u60c5\u51b5\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">\u00b7counts\uff1a\u5bf9\u8ba1\u6570\u6570\u636e\u8fdb\u884c\u5206\u6790\uff08\u8fdb\u4e00\u6b65\u8bc4\u4f30RNA-seq\u6570\u636e\uff09\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">\u00b7multi-bamqc\uff1a\u6bd4\u8f83\u591a\u4e2aNGS\u6d4b\u5e8f\u6570\u636e\u7684\u8d28\u91cf\u63a7\u5236\u62a5\u544a\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">\u00b7clustering\uff1a\u805a\u7c7b\u8868\u89c2\u9057\u4f20\u4fe1\u53f7\u3002<\/span><\/code><code><span class=\"code-snippet_outer\">\u00b7comp-counts\uff1a\u8ba1\u7b97\u7279\u5f81\u8ba1\u6570\u3002<\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.44510385756676557\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_FjKuL5ZGB3uw5p9klibaiagHCP6HsQ.png?resize=553%2C246\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOMajttTZo9GSLfI2S9hgnFcyPLLFjKuL5ZGB3uw5p9klibaiagHCP6HsQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1011\" height=\"246 .47000122070312\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-size: 15px;\">\u4e0b\u9762\u6211\u4eec\u6765\u505a\u4e00\u4e2a\u4f7f\u7528Qualimap\u8fdb\u884cBAM\u8d28\u91cf\u63a7\u5236\u5206\u6790\u7684\u793a\u4f8b\uff1a<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"properties\"><code><span class=\"code-snippet_outer\"># \u8fd0\u884cBAM QC\u5206\u6790<\/span><\/code><code><span class=\"code-snippet_outer\">qualimap bamqc -bam example.bam -outdir output_dir -outformat PDF:HTML<\/span><\/code><code><span class=\"code-snippet_outer\"># \u8fd0\u884cBAM QC\u5206\u6790\u5e76\u6307\u5b9a\u53c2\u8003\u57fa\u56e0\u7ec4<\/span><\/code><code><span class=\"code-snippet_outer\">qualimap bamqc -bam example.bam -outdir output_dir -outformat PDF:HTML -gff example.gff<\/span><\/code><code><span class=\"code-snippet_outer\">\u51fa\u73b0\u4e86\u9519\u8bef\/(\u3112o\u3112)\/~~<\/span><\/code><\/pre>\n<\/section>\n<pre><p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.40370370370370373\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_VnWH90etvzlMpeGQ8YwroJ1lW6xu4g.png?resize=553%2C223\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSO7JAWCk63CibE5OvibR89EjWUO1VnWH90etvzlMpeGQ8YwroJ1lW6xu4g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"223 .739990234375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/> <\/p><\/pre>\n<p><span style=\"font-size: 15px;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u9519\u8bef\u4fe1\u606f\u663e\u793a\u56e0\u4e3a\u5185\u5b58\u4e0d\u8db3\uff0c<\/span><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">Java<span style=\"font-size: 15px;\">\u5185\u5b58\u5927\u5c0f\u88ab\u8bbe\u7f6e\u4e3a<\/span><span style=\"font-size: 15px;\">1200M<\/span><span style=\"font-size: 15px;\">\uff0c\u5728\u8fd0\u884c\u8fc7\u7a0b\u4e2d\u51fa\u73b0\u4e86\u5185\u5b58\u4e0d\u8db3\u7684\u8b66\u544a\u3002<\/span><\/span><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u90a3\u4e48\u589e\u5927\u5185\u5b58\u5e03\u5c40\u597d\u4e86\u5417\uff1f\u679c\u7136\u4e0d\u51fa\u5c0f\u679c\u6240\u6599\uff0c\u589e\u52a0<\/span><span style=\"font-size: 15px;\">–java-mem-size=4G<\/span><span style=\"font-size: 15px;\">\u540e\u518d\u6b21\u8fd0\u884c\uff0c\u8fd9\u4e2a\u95ee\u9898\u89e3\u51b3\u4e86<\/span><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"> <span style=\"font-size: 15px;\">o(*<\/span><span style=\"font-size: 15px;\">\uffe3\u25bd\uffe3<\/span><span style=\"font-size: 15px;\">*)<\/span><span style=\"font-size: 15px;\">\u30d6\uff0c\u4f46\u662f\u9519\u8bef\u53c8\u6765\u4e86\u3002<\/span><\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">qualimap<\/span> bamqc -bam example.bam --java-mem-size=<span class=\"code-snippet__number\">4G<\/span> -outdir output_dir -outformat PDF:HTML -gff example.gff<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-size: 15px;letter-spacing: 0.034em;\">\u9519\u8bef\u4fe1\u606f\u663e\u793a\u5728\u8ba1\u7b97\u62a5\u544a\u65f6\uff0c\u7a0b\u5e8f\u51fa\u73b0\u4e86\u4e00\u4e2a\u5f02\u5e38\uff1a\u65e0\u6cd5\u8fde\u63a5\u5230X11\u7a97\u53e3\u670d\u52a1\u5668\u3002\u8fd9\u4e2a\u95ee\u9898\u53ef\u80fd\u662f\u7531\u4e8eJava\u865a\u62df\u673a\u4f7f\u7528DISPLAY\u73af\u5883\u53d8\u91cf\u6765\u68c0\u6d4bX11\u7cfb\u7edf\u662f\u5426\u53ef\u7528\uff0c\u4f46\u6709\u65f6\u8fd9\u4e2a\u53d8\u91cf\u88ab\u64cd\u4f5c\u7cfb\u7edf\u6216\u67d0\u4e9b\u5e94\u7528\u7a0b\u5e8f\u9519\u8bef\u5730\u8bbe\u7f6e\u4e86\u3002    <\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u8981\u89e3\u51b3\u8fd9\u4e2a\u95ee\u9898\uff0c\u53ef\u4ee5\u5c1d\u8bd5\u53d6\u6d88\u8bbe\u7f6eDISPLAY\u53d8\u91cf\uff0c\u65b9\u6cd5\u662f\u5728\u547d\u4ee4\u884c\u4e2d\u8f93\u5165unset DISPLAY\u6216export DISPLAY=:0\u3002\u6b64\u5916\uff0c\u4f60\u8fd8\u53ef\u4ee5\u4f7f\u7528Java\u7684\u4e00\u4e2a\u7279\u6b8a\u9009\u9879-Djava.awt.headless=true\u6765\u7981\u7528\u663e\u793a\u8981\u6c42\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100012394\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_IWicymEetCc6Enf2BkUE4Yz1Ya5e5w-4.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyuYsqlJnQUJvnS2iciaDibtfbdV0VmHlriaSjYwjFPLp1BLjokHIWicymEetCc6Enf2BkUE4Yz1Ya5e5w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"letter-spacing: 0.578px;text-wrap: wrap;text-align: center;\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u8981\u542f\u7528\u8fd9\u4e2a\u9009\u9879\uff0c\u4f60\u53ef\u4ee5\u5728\u7cfb\u7edf\u4e2d\u8bbe\u7f6eJAVA_OPTS\u53d8\u91cf\uff0c\u6216\u8005\u5728qualimap\u811a\u672c\u4e2d\u4fee\u6539java_options\u53d8\u91cf\uff0c\u4f8b\u5982\uff1a<\/span><\/p>\n<pre><section class=\"code-snippet__fix code-snippet__js\"><ul class=\"code-snippet__line-index code-snippet__js\"><li><\/li><li><\/li><\/ul><pre class=\"code-snippet__js\" data-lang=\"ini\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">java_options<\/span>=<span class=\"code-snippet__string\">\"-Djava.awt.headless=true -Xmx$JAVA_MEM_SIZE -XX:MaxPermSize=1<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">024m\"<\/span><\/code><\/pre>\n<\/section>\n<section><code><span class=\"code-snippet_outer\"><\/span><\/code><\/section>\n<p><span style=\"font-size: 15px;\">\u8fd9\u91cc\u6211\u9009\u62e9\u4e86\u4fee\u6539java_options\u53d8\u91cf\uff0c\u518d\u6b21\u8fd0\u884c\u4e0a\u9762\u7684\u547d\u4ee4\uff0c\u8fd9\u6b21\u6210\u529f\u5566\uff01\u751f\u6210\u4e86\u4e00\u4e2a\u7ed3\u679c\u6587\u4ef6\u5939output_dir\uff0c\u91cc\u9762\u5305\u542b\u4e863\u7ed9\u6587\u4ef6\u548c3\u7ed9\u6587\u4ef6\u5939\uff0c\u5176\u4e2d\u6211\u4eec\u4e3b\u8981\u770bqualimapReport.html\u7f51\u9875\u6587\u4ef6\u3002<\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.88125\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_pvvGXxF2zmia6mAVw5LKErcUdia3dQ.gif?resize=105%2C93\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_gif\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOH7QQicp38eVnFQ0q3OPe4ek4ST2pvvGXxF2zmia6mAVw5LKErcUdia3dQ\/640?wx_fmt=gif'\" data-type=\"gif\" data-w=\"320\" height=\"93 .07000732421875\" width=\"105 .60000610351562\" data-recalc-dims=\"1\" \/><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.023148148148148147\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_1ZPAVYgc3bLwXms3BvgKa9vfPE8NZg.png?resize=553%2C12\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOPnvxIPte5dbT0dvL1oSxYicJn1ZPAVYgc3bLwXms3BvgKa9vfPE8NZg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"12 .5999755859375\" style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-size: 15px;\">qualimapReport.html\u7f51\u9875\u6587\u4ef6\u4e2d\u5305\u542b\u8868\u683c\u5f62\u5f0f\u7684\u7edf\u8ba1\u4fe1\u606f\u548c\u56fe\u7247\u4fe1\u606f\u3002<\/span><\/p>\n<p><span style=\"color: rgb(2, 30, 170);\"><strong><span style=\"font-size: 15px;\">\u8868\u683c\u4fe1\u606f\u5305\u62ec\uff1a<\/span><\/strong><\/span><span style=\"font-size: 15px;\"><\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u5168\u5c40\u90e8\u5206\u5305\u542b\u6709\u5173\u8bfb\u6570\u603b\u6570\u3001\u6620\u5c04\u8bfb\u6570\u6570\u3001\u6210\u5bf9\u7aef\u6620\u5c04\u6027\u80fd\u3001\u8bfb\u957f\u5206\u5e03\u3001\u526a\u5207\u8bfb\u6570\u6570\u548c\u91cd\u590d\u7387\uff08\u6839\u636e\u8bfb\u5bf9\u9f50\u7684\u8d77\u59cb\u4f4d\u7f6e\u4f30\u7b97\uff09\u7684\u4fe1\u606f\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">ACGT\u5185\u5bb9\u90e8\u5206\u62a5\u544a\u6620\u5c04\u8bfb\u6570\u4e2d\u7684\u6838\u82f7\u9178\u542b\u91cf\u548cGC\u767e\u5206\u6bd4\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u8986\u76d6\u90e8\u5206\u63d0\u4f9b\u8986\u76d6\u6df1\u5ea6\u7684\u5e73\u5747\u503c\u548c\u6807\u51c6\u5dee\u7684\u4fe1\u606f\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u6620\u5c04\u8d28\u91cf\u90e8\u5206\u62a5\u544a\u6620\u5c04\u8bfb\u6570\u7684\u5e73\u5747\u6620\u5c04\u8d28\u91cf\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u63d2\u5165\u5927\u5c0f\u90e8\u5206\u63d0\u4f9b\u6709\u5173\u63d2\u5165\u5927\u5c0f\u5206\u5e03\u7684\u5e73\u5747\u503c\u3001\u6807\u51c6\u5dee\u548c\u767e\u5206\u4f4d\u6570\u7684\u4fe1\u606f\uff08\u5982\u679c\u9002\u7528\uff09\uff0c\u57fa\u4e8eSAM\u6587\u4ef6\u7684TLEN\u5b57\u6bb5\u3002   <\/span><o:page><\/o:page><\/p>\n<p><span style=\"font-size: 15px;\">\u9519\u914d\u548c\u63d2\u5165\/\u7f3a\u5931\u90e8\u5206\u62a5\u544a\u4e00\u822c\u5bf9\u9f50\u9519\u8bef\u7387\u3001\u9519\u914d\u603b\u6570\u548c\u63d2\u5165\/\u7f3a\u5931\u603b\u6570\uff0c\u4eceCIGAR\u503c\u8ba1\u7b97\u3002\u6b64\u5916\uff0c\u8fd8\u63d0\u4f9b\u4e86\u540c\u805a\u7269\u63d2\u5165\/\u7f3a\u5931\u5728\u603b\u63d2\u5165\/\u7f3a\u5931\u4e2d\u7684\u6bd4\u4f8b\u3002\u8bf7\u6ce8\u610f\uff0c\u9519\u8bef\u7387\u548c\u9519\u914d\u5ea6\u91cf\u57fa\u4e8eSAM\u8bb0\u5f55\u7684\u53ef\u9009\u5b57\u6bb5\uff08NM\u7528\u4e8e\u7f16\u8f91\u8ddd\u79bb\uff0cMD\u7528\u4e8e\u9519\u914d\uff09\uff0c\u5982\u679c\u8fd9\u4e9b\u5b57\u6bb5\u5728SAM\u6587\u4ef6\u4e2d\u7f3a\u5931\uff0c\u5219\u4e0d\u62a5\u544a\u8fd9\u4e9b\u7279\u5f81\u3002\u67d3\u8272\u4f53\u7edf\u8ba1\u90e8\u5206\u63d0\u4f9b\u6709\u5173\u6bcf\u4e2a\u67d3\u8272\u4f53\uff08\u7531SAM\u6587\u4ef6\u5934\u5b9a\u4e49\uff09\u7684\u6620\u5c04\u78b1\u57fa\u6570\u3001\u8986\u76d6\u6df1\u5ea6\u7684\u5e73\u5747\u503c\u548c\u6807\u51c6\u5dee\u7684\u4fe1\u606f\u3002\u5bf9\u4e8e\u57fa\u4e8e\u533a\u57df\u7684\u5206\u6790\uff0c\u533a\u57df\u5185\u63d0\u4f9b\u989d\u5916\u4fe1\u606f\uff0c\u4f8b\u5982\u6b63\u786e\u94fe\u8bfb\u6570\u7684\u6570\u91cf\u3002<\/span><\/p>\n<p><span style=\"color: rgb(2, 30, 170);font-size: 15px;\"><strong>\u56fe\u7247\u4fe1\u606f\u5305\u62ec\uff1a<\/strong><\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u8986\u76d6\u7387\u5206\u5e03\u56fe\u3002\u7531\u4e24\u4e2a\u56fe\u5f62\u7ec4\u6210\uff0c\u4e0a\u56fe\u663e\u793a\u4e86\u53c2\u8003\u5e8f\u5217\u7684\u8986\u76d6\u7387\u5206\u5e03\uff08\u7ea2\u7ebf\uff09\u548c\u8986\u76d6\u7387\u504f\u5dee\u3002\u4e0b\u56fe\u663e\u793a\u4e86\u53c2\u8003\u5e8f\u5217\u7684GC\u542b\u91cf\uff08\u9ed1\u7ebf\uff09\u53ca\u5176\u5e73\u5747\u503c\uff08\u7ea2\u8272\u865a\u7ebf\uff09\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u8986\u76d6\u7387\u76f4\u65b9\u56fe\u663e\u793a\u5177\u6709\u7ed9\u5b9a\u8986\u76d6\u7387\u7684\u57fa\u56e0\u7ec4\u4f4d\u7f6e\u6570\u3002x\u8f74\u7684\u7bb1\u901a\u8fc7\u805a\u5408\u4e00\u4e9b\u8986\u76d6\u503c\u6765\u65b9\u4fbf\u5730\u7f29\u653e\uff0c\u4ee5\u4fbf\u5728\u5b58\u5728\u5e38\u89c1NGS\u8986\u76d6\u5cf0\u503c\u7684\u60c5\u51b5\u4e0b\u4e5f\u80fd\u4ea7\u751f\u5177\u6709\u4ee3\u8868\u6027\u7684\u76f4\u65b9\u56fe\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u8986\u76d6\u7387\u76f4\u65b9\u56fe\uff080-50X\uff09\u663e\u793a\u5177\u6709\u7ed9\u5b9a\u8986\u76d6\u7387\u7684\u57fa\u56e0\u7ec4\u4f4d\u7f6e\u6570\u3002\u5728\u8fd9\u5f20\u56fe\u4e2d\uff0c\u8986\u76d6\u7387\u5927\u4e8e50X\u7684\u57fa\u56e0\u7ec4\u4f4d\u7f6e\u88ab\u5206\u7ec4\u5230\u6700\u540e\u4e00\u4e2a\u7bb1\u4e2d\u3002\u8fd9\u6837\u505a\u53ef\u4ee5\u83b7\u5f97\u5bf9\u8986\u76d6\u7387\u6700\u5e38\u89c1\u503c\u7684\u66f4\u9ad8\u5206\u8fa8\u7387\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u57fa\u56e0\u7ec4\u5206\u8986\u76d6\u56fe\u3002\u4e86\u89e3\u81f3\u5c11\u5177\u6709\u7ed9\u5b9a\u8986\u76d6\u7387\u7684\u53c2\u8003\u5e8f\u5217\u88ab\u6d4b\u5e8f\u4e86\u591a\u5c11\u3002\u8fd9\u5f20\u56fe\u5e94\u8be5\u6309\u7167\u4ee5\u4e0b\u793a\u4f8b\u8fdb\u884c\u89e3\u91ca\uff1a<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u5982\u679c\u76ee\u6807\u662f\u81f3\u5c1125X\u7684\u8986\u76d6\u7387\uff08x\u8f74\uff09\uff0c\u90a3\u4e48y\u8f74\u5e94\u8be5\u662f\u7ea683\uff05\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u91cd\u590d\u7387\u76f4\u65b9\u56fe\u3002\u663e\u793a\u91cd\u590d\u8bfb\u53d6\u8d77\u59cb\u4f4d\u7f6e\u7684\u5206\u5e03\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">\u6620\u5c04reads\u4e0a\u7684\u6838\u82f7\u9178\u542b\u91cf\u8d34\u56fe\u3002\u6b64\u56fe\u663e\u793a\u6620\u5c04\u5230reads\u6bcf\u4e2a\u4f4d\u7f6e\u7684\u6838\u82f7\u9178\u542b\u91cf\u3002<\/span><\/p>\n<p><span style=\"font-size: 15px;\">GC\u542b\u91cf\u5206\u5e03\u56fe\u3002\u6b64\u56fe\u663e\u793a\u6bcf\u4e2a\u6620\u5c04\u8bfb\u6570\u7684GC\u542b\u91cf\u5206\u5e03\u3002\u5982\u679c\u4e0e\u9884\u5148\u8ba1\u7b97\u7684\u57fa\u56e0\u7ec4\u5206\u5e03\u8fdb\u884c\u6bd4\u8f83\uff0c\u6b64\u56fe\u53ef\u4ee5\u68c0\u67e5\u662f\u5426\u5b58\u5728GC\u542b\u91cf\u504f\u79fb\u3002   <\/span><o:page><\/o:page><\/p>\n<p><span style=\"font-size: 15px;\">\u5982\u679c\u4f60\u60f3\u4e86\u89e3\u66f4\u591a\u7684\u4fe1\u606f\uff0c\u53ef\u4ee5\u53c2\u8003qualimap\u6587\u6863\uff0c\u7f51\u5740\u662f\uff1a<\/span><span style=\"font-size: 15px;\">http:\/\/qualimap.conesalab.org\/doc_html\/index.html#<\/span><span style=\"font-size: 15px;\"> \u3002<\/span><span style=\"font-size: 15px;letter-spacing: 0.034em;\"><\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.7574074074074074\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_8bej9Ktgkn40RavflaoDI4oDpyHnHQ.png?resize=539%2C408\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSO9ic1FnIQQwy0KEGnuZBkWSmUib8bej9Ktgkn40RavflaoDI4oDpyHnHQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"408 .530029296875\" width=\"539 .2100219726562\" data-recalc-dims=\"1\" \/><span style=\"font-size: 15px;letter-spacing: 0.034em;\">    <\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-ratio=\"0.7361111111111112\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_DvrdHOTJCcOhrEfVlDufuric49swLA.png?resize=486%2C358\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAwDacKVXGoNdTpEREpc6gSOYKcbaaxSFhaiccmMLcsw3dYjGJDvrdHOTJCcOhrEfVlDufuric49swLA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"358 .4599914550781\" width=\"486 .77001953125\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"color: rgb(2, 30, 170);font-size: 15px;\"><strong>\u53c2<\/strong><\/span><span style=\"color: rgb(2, 30, 170);font-size: 15px;\"><strong>\u8003\u6587<\/strong><\/span><span style=\"color: rgb(2, 30, 170);font-size: 15px;\"><strong>\u732e\uff1a<\/strong><\/span><span style=\"font-size: 15px;\">Okonechnikov, K., Conesa, A., & Garc\u00eda-Alcalde, F. (2015). \u201cQualimap 2: advanced multi-sample quality control for high-throughput sequencing data.\u201d Bioinformatics, btv566<\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;white-space: normal;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);text-align: left;\">\n<section style=\"outline: 0px;font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;outline: 0px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;outline: 0px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;outline: 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href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" rel=\"noopener\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/a><br style=\"outline: 0px;\" \/><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" rel=\"noopener\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/a><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491909&idx=1&sn=902e34bcf53e605fba2b457ec6a33e36&chksm=cf7f7180f808f8961542f458a49da14e85d8831449fb65fbf2623d55affc564686a5422f56fd&scene=21#wechat_redirect\" textvalue=\"3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" rel=\"noopener\">3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8<\/a><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491905&idx=1&sn=fb34ebd6282962c7803badaee7a39096&chksm=cf7f7184f808f8929608c0050344ad86193d0fc6455c1c18e16cc17bf4b37832b63d3b18284e&scene=21#wechat_redirect\" textvalue=\"4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;\" rel=\"noopener\"><span style=\"outline: 0px;font-size: 14px;\">4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b<\/span><\/a><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493633&idx=1&sn=2263dfe7572c0bcf6816c2c1a37d79de&chksm=cf7f78c4f808f1d2209321d6ace39fde5fa60c0691b56f88eac21916f5a2872ce8ee345f1d5f&scene=21#wechat_redirect\" textvalue=\"\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;letter-spacing: 0.578px;font-size: 14px;\" rel=\"noopener\">5.\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01<\/a><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-ratio=\"0.5555555555555556\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_VmRkv7tSNKliafDnsuFR7BoeDgBrww-1.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxPqeNQsFjm2HibIuSld9Nh9GicQSWhJwHjia5tEevQvDIebLErVmRkv7tSNKliafDnsuFR7BoeDgBrww\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"900\" style=\"\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"margin-top: 0pt;margin-bottom: 0pt;margin-left: 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