{"id":60131,"date":"2024-06-17T10:59:07","date_gmt":"2024-06-17T02:59:07","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=60131"},"modified":"2024-06-17T10:59:07","modified_gmt":"2024-06-17T02:59:07","slug":"%e8%b7%9f%e7%9d%80%e9%ab%98%e5%88%86nature%e5%ad%a6%e5%a4%8d%e7%8e%b0-%e5%8d%95%e7%bb%86%e8%83%9e%e8%bd%ac%e5%bd%95%e7%bb%84%e8%82%bf%e7%98%a4%e5%be%ae%e7%8e%af%e5%a2%83%e6%8b%bf","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=60131","title":{"rendered":"\u8ddf\u7740\u9ad8\u5206Nature\u5b66\u590d\u73b0\u2014\u2014\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4+\u80bf\u7624\u5fae\u73af\u5883\u62ff\u4e0b\u4e8c\u533a16.6\u5206\u6587\u7ae0"},"content":{"rendered":"


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normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;\">\u300a<\/span><span style=\"text-decoration: underline;letter-spacing: 0.034em;font-family: \u7b49\u7ebf;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;font-weight: bold;\">Single cell transcriptomic analyses implicate an immunosuppressive tumor microenvironment in pancreatic cancer liver metastasis<\/span><span style=\"text-decoration: underline;letter-spacing: 0.034em;font-family: \u5b8b\u4f53;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;\">\u300b<\/span><\/span><\/em><span style=\"letter-spacing: 0.034em;font-family: \u5b8b\u4f53;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;\">IF16.6\uff0c\u8fd9\u4e48\u9ad8\u5206\u7684\u6587\u7ae0\uff0c<span 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wrap;\">\u5c0f\u4e91<\/span>\u53d1\u73b0\u8fd9\u662f\u7531\u4e24\u79cd\u56fe\u7ec4\u6210\uff0c\u4e00\u4e2a\u662f\u5806\u53e0\u7684\u5c0f\u63d0\u7434\u56fe\uff0c\u53f3\u8fb9\u662f\u6c14\u6ce1\u70ed\u56fe\uff0c\u597d\u4e86\u6211\u4eec\u77e5\u9053\u8fd9\u4e24\u4e2a\u7ec4\u56fe\u7684\u540d\u5b57\u4e4b\u540e\u5c31\u662f\u53bb\u5b9e\u73b0\u4ed6\u4eec\u3002<\/span><span style=\"font-family: \u7b49\u7ebf;letter-spacing: 0.034em;\">       <\/span><\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: 14pt;letter-spacing: 0.034em;\"><span style=\"font-size: 16px;\"> <\/span><\/o:p><\/p>\n<p><span style=\"font-size: 16px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u6211\u4eec\u4ece\u53f3\u8fb9\u7684\u56fe\u4e0b\u624b\uff0c\u56e0\u4e3a\u8fd9\u4e2a\u6587\u7ae0\u662f\u5355\u7ec6\u80de\u7684\u6587\u7ae0\uff0c<span style=\"font-family: \u5b8b\u4f53;letter-spacing: 0.634667px;text-wrap: wrap;\">\u5c0f\u4e91<\/span>\u81ea\u5df1\u4e5f\u7528\u4e86\u81ea\u5df1\u5355\u7ec6\u80de\u6570\u636e\uff0c\u5c0f\u4f19\u4f34\u53ef\u4ee5\u5957\u7528\u6570\u636e\u683c\u5f0f\u53bb\u590d\u73b0\u54e6\u3002<\/span><span style=\"color: rgb(61, 170, 214);\"><strong><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u60f3\u8981\u4e00\u8d77\u5b66\u4e60\u590d\u73b0\u7684\u5c0f\u4f19\u4f34<\/span><span style=\"letter-spacing: 0.034em;font-family: \u5b8b\u4f53;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;\">\u53ef\u4ee5<\/span><\/strong><\/span><span style=\"color: rgb(217, 33, 66);\"><strong><span style=\"letter-spacing: 0.034em;font-family: \u5b8b\u4f53;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;\">\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c1125\u201d\u9886\u53d6\u4ee3\u7801\u548c\u793a\u4f8b\u6570\u636e<\/span><\/strong><\/span><span style=\"color: rgb(61, 170, 214);\"><strong><span style=\"letter-spacing: 0.034em;font-family: \u5b8b\u4f53;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;\">\uff0c\u5982\u679c\u5b66\u4e0d\u4f1a\uff0c\u53ef\u4ee5\u627e\u6211\u4eec\u5e2e\u60a8\u590d\u73b0\uff0c\u5c0f\u4e91\u4e0d\u53ea\u4f1a\u505a\u56fe\uff0c\u5f88\u591a\u9ad8\u5206\u6587\u732e\u601d\u8def\u90fd\u53ef\u4ee5\uff01       <\/span><\/strong><\/span><\/span><o:p style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;\"><span style=\"font-size: 16px;\"><span style=\"color: rgb(61, 170, 214);\"><strong><span style=\"font-family: \u5b8b\u4f53;font-variant-numeric: normal;font-variant-east-asian: normal;font-variant-alternates: normal;font-variant-position: normal;\"> <\/span><\/strong><\/span><\/span><\/o:p><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"503296887\" data-ratio=\"1.005281690140845\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_0UicKqQUrnEGEeIJUUXKODzMI5Mz4g.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrHtT96Zu0E51zQl2vTF1ibvYxtFlcg0ibgBjxI00wVgofXSDDg0UicKqQUrnEGEeIJUUXKODzMI5Mz4g\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"568\" style=\"width: 176px;height: 177px;\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-size: 16px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u6211\u4eec\u5f00\u59cb\u8f7d\u5165\u51e0\u4e2a\u5305\uff1a<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\">library(scRNAtoolVis) <span class=\"code-snippet__comment\"># \u5c0f\u4e91\u52a0\u8f7d\u8fd9\u4e2a\u5305\uff0c\u4e3a\u4e86\u4f7f\u7528\u8fd9\u4e2a\u5305\u7684\u6570\u636e\uff0c <\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(Seurat) <span class=\"code-snippet__comment\"># dotplot\uff0c\u5355\u7ec6\u80de\u7ed8\u56fe\u4f7f\u7528<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(cowplot) <span class=\"code-snippet__comment\"># plot_grid<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">httest <- system.file(<span class=\"code-snippet__string\">\"extdata\"<\/span>, <span class=\"code-snippet__string\">\"htdata.RDS\"<\/span>, <span class=\"code-snippet__keyword\">package<\/span> = <span class=\"code-snippet__string\">\"scRNAtoolVis\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- readRDS(httest)          <\/span><\/code><\/pre>\n<\/section>\n<p><o:p style=\"font-family: \u7b49\u7ebf;font-size: 14pt;letter-spacing: 0.034em;\"><span style=\"font-size: 16px;\"><\/span><\/o:p><\/p>\n<p><span style=\"font-size: 16px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u7136\u540e\u6211\u4eec\u8f7d\u5165\u6570\u636e<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6dfb\u52a0\u5206\u7ec4 <\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc$groups <- rep(c(<span class=\"code-snippet__string\">'stim'<\/span>,<span class=\"code-snippet__string\">'control'<\/span>),each = <span class=\"code-snippet__number\">1319<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6dfb\u52a0\u7ec6\u80de\u7c7b\u578b<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc$celltype <- Seurat::Idents(pbmc)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u8f7d\u5165 markergene<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">data(<span class=\"code-snippet__string\">\"top3pbmc.markers\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296888\" data-ratio=\"0.9345454545454546\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_TJGicsFBbkQp67Mtd4iccSSNGicccg.png?resize=549%2C513\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1JYdicsqNfMYTEDP2TAhSQI0LyREJJTJGicsFBbkQp67Mtd4iccSSNGicccg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"550\" height=\"513 .9400634765625\" width=\"549 .8800048828125\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u8fd9\u4e2a\u5176\u5b9e\u5c31\u662f\u6211\u4eec\u4e00\u822c\u5355\u7ec6\u80de\u64cd\u4f5c\u5b98\u65b9\u6d41\u7a0b\u540e\uff0c\u8dd1\u5b8c\u7b2c\u4e00\u4e2a\u6570\u636e\u7ed3\u679c\uff0c\u5c0f\u4f19\u4f34\u53ef\u4ee5\u76f4\u63a5\u4f7f\u7528<\/span><\/p>\n<p><span style=\"font-size: 16px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u540e\u9762\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528\u8fd9\u4e2a\u6570\u636e\u53bb\u7ed8\u5236\u56fe\u7247<\/span>    <\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\"># check<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span style=\"font-size: 16px;\">head(top3pbmc.markers,3)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296886\" data-ratio=\"0.260347129506008\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_icjGsUfhjgTl058MhiaxeOiabLdWMw.png?resize=553%2C144\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1J2OvaajIuHrTRudNh5LmIVwiaJ1o1icjGsUfhjgTl058MhiaxeOiabLdWMw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"749\" height=\"144 .13999938964844\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">p<\/span> <- DotPlot(pbmc,<\/span><\/code><code><span class=\"code-snippet_outer\"> features = split(top3pbmc.markers<span class=\"code-snippet__variable\">$gene<\/span>, top3pbmc.markers<span class=\"code-snippet__variable\">$cluster<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> cols = c(<span class=\"code-snippet__string\">\"#dddddd\"<\/span>, <span class=\"code-snippet__string\">\"#448444\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">) +<\/span><\/code><code><span class=\"code-snippet_outer\"> RotatedAxis() + <span class=\"code-snippet__comment\"># \u6765\u81eaSeurat<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> theme(<\/span><\/code><code><span class=\"code-snippet_outer\"> panel.border = element_rect(color = <span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> panel.spacing = unit(<span class=\"code-snippet__number\">1<\/span>, <span class=\"code-snippet__string\">\"mm\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.title = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text.y = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> )<span class=\"code-snippet__comment\">#\u8bbe\u7f6e\u4e3b\u9898\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">p<span class=\"code-snippet__variable\">$data<\/span><span class=\"code-snippet__variable\">$feature<\/span>.groups2 <- factor(p<span class=\"code-snippet__variable\">$data<\/span><span class=\"code-snippet__variable\">$feature<\/span>.groups, <\/span><\/code><code><span class=\"code-snippet_outer\"> levels = c(<span class=\"code-snippet__string\">\"Platelet\"<\/span>,<span class=\"code-snippet__string\">\"DC\"<\/span>,<span class=\"code-snippet__string\">\"NK\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__string\">\"FCGR3A+ Mono\"<\/span>,<span class=\"code-snippet__string\">\"CD8 T\"<\/span>,<span class=\"code-snippet__string\">\"B\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__string\">\"Memory CD4 T\"<\/span>,<span class=\"code-snippet__string\">\"CD14+ Mono\"<\/span>,<span class=\"code-snippet__string\">\"Naive CD4 T\"<\/span>))<span class=\"code-snippet__comment\">#\u6ce8\u91ca\u5355\u7ec6\u80de\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">P<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296895\" data-ratio=\"0.43425925925925923\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_bPFjj3ceibUjFu9cfSTsS6AlWLZHeQ.png?resize=553%2C240\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1JsYjnyuPI5BH3iasYRJUHZbcZXzibPFjj3ceibUjFu9cfSTsS6AlWLZHeQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"240 .5999755859375\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">          <o:p> <\/o:p><\/span><\/p>\n<p><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u8fd9\u6837\u4e00\u6765\u6587\u7ae0\u4e2d\u53f3\u8fb9\u7684\u56fe\u5c31\u5927\u81f4\u7684\u7ed8\u5236\u51fa\u6765\u4e86\uff0c\u4e0a\u9762<span style=\"font-variant: normal;text-transform: none;font-family: \u5b8b\u4f53;letter-spacing: 0.634667px;text-wrap: wrap;\">\u5c0f\u4e91<\/span>\u8fd8\u52a0\u4e86\u5355\u7ec6\u80de\u7684\u6ce8\u91ca\u7ed3\u679c\uff0c\u66f4\u52a0\u4e30\u5bcc\uff0c\u4f46\u662f\u53d1\u73b0\u6709\u4e00\u4e9b\u5c0f\u7ec6\u8282\u6ca1\u6709\u5904\u7406\u5230\u4f4d\uff0c\u4e2d\u95f4\u8fd8\u6709\u5206\u5272\u7684\u7ebf\uff0c\u4e0b\u9762\u6211\u4eec\u5728\u7ee7\u7eed\u7ed8\u5236\uff0c\u6211\u4eec\u5bf9\u7ec6\u8282\u7684\u4fee\u6539\u9700\u8981\u7528\u5230ggplot2\u4e2d\u7684\u53c2\u6570\u5904\u7406\u6765\u770b\u770b\u5982\u4f55\u5904\u7406\u3002<\/span><span style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 0.034em;\">     <\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 0.034em;\"> <\/o:p><span style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 0.034em;\"><\/span><\/p>\n<p><span style=\"font-size: 16px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u4f7f\u7528ggplot2\u4ece\u5934\u7ed8\u5236<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"bash\"><code><span class=\"code-snippet_outer\">library(ggh4x)<span class=\"code-snippet__comment\">#\u9700\u8981\u7528\u5230\u91cc\u9762\u7684\u51fd\u6570\u5904\u7406\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(RColorBrewer)<span class=\"code-snippet__comment\">#\u52a0\u8f7d\u8fd9\u4e2a\u5305\u53ea\u662f\u4e3a\u4e86\u586b\u5145\u989c\u8272 <\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e0b\u9762\u628a\u6570\u636e\u586b\u5145\u4e00\u4e0b\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">strip <- strip_themed(<\/span><\/code><code><span class=\"code-snippet_outer\"> background_x = elem_list_rect(fill = brewer.pal(9, <span class=\"code-snippet__string\">\"Paired\"<\/span>)))<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e0b\u9762\u7ed8\u5236\u56fe\u7247\uff0c\u4ece\u4e3b\u9898\u989c\u8272\u7b49\uff0c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p<span class=\"code-snippet__variable\">$data<\/span> %>% <\/span><\/code><code><span class=\"code-snippet_outer\"> ggplot(aes(x = features.plot,<\/span><\/code><code><span class=\"code-snippet_outer\"> y = id)) + <\/span><\/code><code><span class=\"code-snippet_outer\"> geom_point(aes(size = pct.exp, <\/span><\/code><code><span class=\"code-snippet_outer\"> color = avg.exp.scaled)) + <\/span><\/code><code><span class=\"code-snippet_outer\"> facet_wrap2(~feature.groups2, <\/span><\/code><code><span class=\"code-snippet_outer\"> scales = <span class=\"code-snippet__string\">\"free_x\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> strip = strip, <\/span><\/code><code><span class=\"code-snippet_outer\"> nrow = 1) + <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e3b\u9898\u7684\u4fee\u6b63<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> theme_classic() + <\/span><\/code><code><span class=\"code-snippet_outer\"> theme(axis.text.x = element_text(angle = 90, <\/span><\/code><code><span class=\"code-snippet_outer\"> hjust = 0.5, <span class=\"code-snippet__comment\">#\u6c14\u6ce1\u95f4\u8ddd<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> vjust = 0.3, <\/span><\/code><code><span class=\"code-snippet_outer\"> color = <span class=\"code-snippet__string\">\"black\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.title = element_blank(),<\/span><\/code><code><span class=\"code-snippet_outer\"> strip.background = element_rect(color = <span class=\"code-snippet__string\">\"white\"<\/span>), <\/span><\/code><code><span class=\"code-snippet_outer\"> axis.text.y = element_blank()) + <\/span><\/code><code><span class=\"code-snippet_outer\">  scale_color_gradient(low = <span class=\"code-snippet__string\">\"#ffffff\"<\/span>,<span class=\"code-snippet__comment\">#\u5e95\u6570\u636e\u989c\u8272\u8bbe\u7f6e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> high = <span class=\"code-snippet__string\">\"#448444\"<\/span>, <\/span><\/code><code><span class=\"code-snippet_outer\"> name = <span class=\"code-snippet__string\">\"avg.exp\"<\/span>) -> p<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e0a\u8ff0\u7684\u7ed8\u56fe\uff0c\u8bb8\u591a\u90fd\u662f\u9ed8\u8ba4\u7684\u53c2\u6570\uff0c\u5c0f\u4f19\u4f34\u53ef\u4ee5\u65b9\u5411\u53bb\u4f7f\u7528<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">P<span class=\"code-snippet__comment\">#\u67e5\u770b\u4e00\u4e0b\u56fe<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296892\" data-ratio=\"0.43703703703703706\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_ayHAdY0Bg7E0bfRGK15RlDlkPLTQEw.png?resize=553%2C242\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1JYsVZ0V8LLcicmyZawYZoEKUlviayHAdY0Bg7E0bfRGK15RlDlkPLTQEw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"242 .19998168945312\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><span style=\"letter-spacing: 0.034em;\">         <\/span><o:p style=\"letter-spacing: 0.034em;\"> <\/o:p><\/span><\/p>\n<p><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u8fd9\u6837\u4e00\u6765\u6211\u4eec\u53f3\u8fb9\u7684\u56fe\u5c31\u590d\u73b0\u7684\u5dee\u4e0d\u591a\uff0c\u5bf9\u6bd4\u4e00\u4e0b<\/span><span style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 0.034em;\">         <\/span><o:p style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 0.034em;\"> <\/o:p><span style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 0.034em;\"><\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296894\" data-ratio=\"0.31493506493506496\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_cBIy6KohiaQxeezw6gMdduwz5v5SkQ.png?resize=553%2C174\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1JibIBkk4zcKicUicicaVDnqJX0VL2KicBIy6KohiaQxeezw6gMdduwz5v5SkQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"924\" height=\"174 .4000244140625\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/p>\n<p><span style=\"font-size: 16px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u53d1\u73b0\u90fd\u5df2\u7ecf\u5dee\u4e0d\u591a\u4e86\uff0c\u53ea\u662f\u989c\u8272\u7684\u8bbe\u7f6e\uff0c\u5c0f\u4f19\u4f34\u5bf9\u6b64\u53ef\u4ee5\u81ea\u884c\u53bb\u8bbe\u7f6e\uff0c\u8fd8\u6709<span style=\"font-family: \u5b8b\u4f53;letter-spacing: 0.634667px;text-wrap: wrap;\">\u5c0f\u4e91<\/span>\u81ea\u5df1\u52a0\u7684\u5355\u7ec6\u80de\u5206\u7ec4\u4fe1\u606f\u5728\u4e0a\u65b9\uff0c\u5c0f\u4f19\u4f34\u4e0d\u559c\u6b22\u8fd9\u4e2a\u5355\u7ec6\u80de\u5206\u7ec4\u53ef\u4ee5\u5220\u9664\uff0c\u53ea\u9700\u8981\u628astrip\u8fd9\u4e2a\u4fe1\u606f\u6ce8\u91ca\u6389\u5c31\u53ef\u4ee5\uff0c\u597d\u4e86\u6211\u4eec\u5b9e\u73b0\u4e86\u53f3\u8fb9\u7684\u56fe\u7247\uff0c\u53bb\u590d\u73b0\u4e00\u4e0b\u5de6\u8fb9\u7684\u5806\u53e0\u5c0f\u63d0\u7434\u56fe\u5427<\/span>    <\/span><o:page><\/o:page><\/p>\n<p><span style=\"font-size: 16px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;\">\u6211\u4eec\u5148\u8f7d\u5165\u4f7f\u7528\u7684\u5305<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code><span class=\"code-snippet_outer\">library(Seurat)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggpubr)<\/span><\/code><code><span class=\"code-snippet_outer\">library(ggsignif)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;font-size: 16px;\">\u8f7d\u5165\u6211\u4eec\u5b9e\u4f8b\u7684\u6570\u636e<\/span><\/p>\n<p><span style=\"font-size: 16px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5176\u5b9e\u5806\u53e0\u5c0f\u63d0\u7434\u56fe\uff0c\u5c0f\u4f19\u4f34\u4e0d\u5c11\u89c1\uff0c\u505a\u5355\u7ec6\u80de\u6570\u636e\u90fd\u4f1a\u7528\u5f97\u5230\uff0c\u6765\u770b\u770b\u5982\u4f55\u7ed8\u5236\u7684<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u52a0\u8f7d\u6570\u636e\u5bf9\u8c61<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span> <- readRDS(<span class=\"code-snippet__string\">\"pbmc_2k_1.rds\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">identity <- readRDS(<span class=\"code-snippet__string\">\"pbmc_2k_2.rds\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296893\" data-ratio=\"0.49941383352872215\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_kXWPqQ7dxOHgUXdSL0tMWxSL2AcEqA.png?resize=553%2C276\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1Jiasbftk9UBHxiaVXPoRNqRRUZ4kXWPqQ7dxOHgUXdSL0tMWxSL2AcEqA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"853\" height=\"276 .53997802734375\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">features<\/span> <- c(<span class=\"code-snippet__string\">\"CD79A\"<\/span>, <span class=\"code-snippet__string\">\"MS4A1\"<\/span>, <span class=\"code-snippet__string\">\"CD8A\"<\/span>, <span class=\"code-snippet__string\">\"CD8B\"<\/span>, <span class=\"code-snippet__string\">\"LYZ\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__string\">\"LGALS3\"<\/span>)      <\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-weight: bold;font-size: 16px;\">\u63d0\u53d6\u51e0\u4e2a\u57fa\u56e0\u7ed8\u5236<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u63d0\u53d6\u6570\u636e\u6846\u5b50\u96c6<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span> <- pbmc[,features]<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296891\" data-ratio=\"0.7831775700934579\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_jB1DEo98TtLiccBK2nuq2pudhYgBzw.png?resize=534%2C418\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1Je71W17t9qbiaC5PTdGWXEYhPfCjB1DEo98TtLiccBK2nuq2pudhYgBzw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"535\" height=\"418 .94000244140625\" width=\"534 .8699951171875\" data-recalc-dims=\"1\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u6dfb\u52a0\u7ec6\u80dename\u548c\u7ec6\u80de\u7fa4\u8eab\u4efd<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc$Cell <- rownames(pbmc)<\/span><\/code><code><span class=\"code-snippet_outer\">pbmc$Idents <- identity<\/span><\/code><code><span class=\"code-snippet_outer\">\u5bf9\u4e0a\u8ff0\u6570\u636e\u5904\u7406\u4e00\u4e0b<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># melt\u6570\u636e\u53d8\u957f<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc <- reshape2::melt(pbmc, <\/span><\/code><code><span class=\"code-snippet_outer\"> id.vars = c(<span class=\"code-snippet__string\">\"Cell\"<\/span>,<span class=\"code-snippet__string\">\"Idents\"<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> measure.vars = features,<\/span><\/code><code><span class=\"code-snippet_outer\"> variable.name = <span class=\"code-snippet__string\">\"Feat\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> value.name = <span class=\"code-snippet__string\">\"Expr\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u67e5\u770b\u6570\u636e\u524d10\u884c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">head(pbmc, <span class=\"code-snippet__number\">10<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296898\" data-ratio=\"0.5540275049115914\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_hibCr7NY1k3Ho8A9frsYgzvGLtR07Q.png?resize=508%2C281\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1Jic3ibIchwfRc3iaGgFzdNyIXZiaEShibCr7NY1k3Ho8A9frsYgzvGLtR07Q\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"509\" height=\"281 .94000244140625\" width=\"508 .94000244140625\" data-recalc-dims=\"1\"><\/span><\/p>\n<p><span style=\"font-size: 16px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u4e0b\u9762\u6211\u4eec\u5c31\u53bb\u7ed8\u5236\u56fe\u7247<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u4e3b\u9898theme\uff08\u5927\u5c0f\uff0c\u989c\u8272\uff09\uff0clegend <\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p2 <- ggplot(pbmc, aes(Feat, Expr, fill = Feat)) +<\/span><\/code><code><span class=\"code-snippet_outer\"> geom_violin(scale = <span class=\"code-snippet__string\">\"width\"<\/span>, adjust = <span class=\"code-snippet__number\">1<\/span>, trim = <span class=\"code-snippet__keyword\">TRUE<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_y_continuous(expand = c(<span class=\"code-snippet__number\">0<\/span>, <span class=\"code-snippet__number\">0<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> position=<span class=\"code-snippet__string\">\"right\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> labels = <span class=\"code-snippet__function\"><span class=\"code-snippet__keyword\">function<\/span><span class=\"code-snippet__params\">(x)<\/span>#\u5bf9\u4e3b\u9898\u6c34\u5e73\u7684\u8bbe\u7f6e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">c<\/span><span class=\"code-snippet__params\">(rep<span class=\"code-snippet__params\">(x = <span class=\"code-snippet__string\">\"\"<\/span>, times = length<span class=\"code-snippet__params\">(x)<\/span><span class=\"code-snippet__number\">-2<\/span>)<\/span>, x[length<span class=\"code-snippet__params\">(x)<\/span> - <span class=\"code-snippet__number\">1<\/span>], <span class=\"code-snippet__string\">\"\"<\/span>)<\/span>) + <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">facet_grid<\/span><span class=\"code-snippet__params\">(rows = vars<span class=\"code-snippet__params\">(Idents)<\/span>, scales = <span class=\"code-snippet__string\">\"free\"<\/span>, switch = <span class=\"code-snippet__string\">\"y\"<\/span>)<\/span> +<\/span><\/code><code><span class=\"code-snippet_outer\">#\u4e0b\u9762\u662f\u5bf9\u4e3b\u9898\u7684\u8bbe\u7f6e<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">theme_cowplot<\/span><span class=\"code-snippet__params\">(font_size = <span class=\"code-snippet__number\">12<\/span>)<\/span> +<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">theme<\/span><span class=\"code-snippet__params\">(legend.position = <span class=\"code-snippet__string\">\"none\"<\/span>,<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> panel.spacing = unit<span class=\"code-snippet__params\">(<span class=\"code-snippet__number\">0<\/span>, <span class=\"code-snippet__string\">\"lines\"<\/span>)<\/span>,#\u56fe\u4e2d\u7ebf\u7684\u8bbe\u7f6e<\/span><\/code><code><span class=\"code-snippet_outer\"> plot.title = element_text<span class=\"code-snippet__params\">(hjust = <span class=\"code-snippet__number\">0.5<\/span>)<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> panel.background = element_rect<span class=\"code-snippet__params\">(fill = NA, color = <span class=\"code-snippet__string\">\"black\"<\/span>)<\/span>,#\u989c\u8272\u586b\u5145<\/span><\/code><code><span class=\"code-snippet_outer\"> plot.margin = margin<span class=\"code-snippet__params\">(<span class=\"code-snippet__number\">6<\/span>, <span class=\"code-snippet__number\">6<\/span>, <span class=\"code-snippet__number\">0<\/span>, <span class=\"code-snippet__number\">6<\/span>, <span class=\"code-snippet__string\">\"pt\"<\/span>)<\/span>,#\u56fe\u4e2d\u5c0f\u63d0\u7434\u56fe\u7684\u95f4\u8ddd<\/span><\/code><code><span class=\"code-snippet_outer\"> strip.background = element_blank<span class=\"code-snippet__params\">()<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> strip.text = element_text<span class=\"code-snippet__params\">(face = <span class=\"code-snippet__string\">\"bold\"<\/span>)<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> strip.text.y.left = element_text<span class=\"code-snippet__params\">(angle = <span class=\"code-snippet__number\">0<\/span>)<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.title.x = element_blank<span class=\"code-snippet__params\">()<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.ticks.x = element_blank<span class=\"code-snippet__params\">()<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet_outer\"> axis.text.x = element_blank<span class=\"code-snippet__params\">()<\/span>)<\/span> +<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">ggtitle<\/span><span class=\"code-snippet__params\">(<span class=\"code-snippet__string\">\"\"<\/span>)<\/span> +#\u6dfb\u52a0\u4e3b\u9898\u540d\u5b57\uff0c\u53ef\u4ee5\u4e0d\u5199<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">ylab<\/span><span class=\"code-snippet__params\">(<span class=\"code-snippet__string\">\"Expression Level\"<\/span>)<\/span>#<span class=\"code-snippet__title\">Y<\/span>\u8f74\u7684\u540d\u79f0<\/span><\/code><code><span class=\"code-snippet_outer\"> #<span class=\"code-snippet__title\">coord_flip<\/span><span class=\"code-snippet__params\">()<\/span>#\u53ef\u4ee5\u4fee\u6539\u6a2a\u7eb5\u5750\u6807<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">p2<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296897\" data-ratio=\"1.1075471698113208\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_9Re4BxY2jQ28dWa6sLibSXlvQGsRfQ.png?resize=529%2C586\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1J2RBOBPyrBleEMW8G4xhMVTfB69Re4BxY2jQ28dWa6sLibSXlvQGsRfQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"530\" height=\"586 .8800048828125\" width=\"529 .9400024414062\" data-recalc-dims=\"1\"><\/span><\/p>\n<p><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u8fd9\u4e2a\u56fe\u662f\u4e0d\u662f\u548c\u6587\u7ae0\u4e2d\u5dee\u4e0d\u591a\u4e86<\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296900\" data-ratio=\"0.5520833333333334\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/13_LU6icAnURhKLUbHPnU9tS2s7tENGHA.png?resize=479%2C264\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1JKmIbLwYkJFD2ogUIlLziby4IYmLU6icAnURhKLUbHPnU9tS2s7tENGHA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"480\" height=\"264 .94000244140625\" width=\"479 .94000244140625\" data-recalc-dims=\"1\"><\/span><span style=\"font-size: 16px;\">    <\/span><o:page><\/o:page><\/p>\n<p><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u4e00\u4e9b\u7ec6\u8282\u8fd8\u6ca1\u5904\u7406\u5230\u4f4d\uff0c\u5c0f\u4f19\u4f34\u8981\u662f\u9700\u8981\u5c06\u5c0f\u63d0\u7434\u53cd\u8f6c\u53ef\u4ee5\u4f7f\u7528coord_flip()<\/span><\/p>\n<p><span style=\"font-size: 16px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u6211\u4eec\u4e0b\u9762\u5bf9\u6570\u636e\u989c\u8272\u7684\u8bbe\u7f6e\u4e00\u4e0b<\/span><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"nginx\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">spring<\/span> <- c(<span class=\"code-snippet__string\">\"#f6bcfd\"<\/span>,<span class=\"code-snippet__string\">\"#8dd3c6\"<\/span>,<span class=\"code-snippet__string\">\"#ffc512\"<\/span>,<span class=\"code-snippet__string\">\"#ffa300\"<\/span>,<span class=\"code-snippet__string\">\"#ff7d00\"<\/span>,<span class=\"code-snippet__string\">\"#ff6581\"<\/span>,<span class=\"code-snippet__string\">\"#f8d90d\"<\/span>,<span class=\"code-snippet__string\">\"#a5da6b\"<\/span>,<span class=\"code-snippet__string\">\"#e578d6\"<\/span>,<span class=\"code-snippet__string\">\"#ffd2d8\"<\/span>,<span class=\"code-snippet__string\">\"#90e4cd\"<\/span>,<span class=\"code-snippet__string\">\"#84dce0\"<\/span>,<span class=\"code-snippet__string\">\"#fe65b3\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">\u5bf9\u989c\u8272\u8d4b\u503c<\/span><\/code><code><span class=\"code-snippet_outer\">library(scales)<\/span><\/code><code><span class=\"code-snippet_outer\"><br \/><\/span><\/code><code><span class=\"code-snippet_outer\">show_col(spring,ncol=<span class=\"code-snippet__number\">5<\/span>)<span class=\"code-snippet__comment\">#\u67e5\u770b\u4e00\u4e0b\u6709\u90a3\u4e9b\u989c\u8272<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296896\" data-ratio=\"0.6730769230769231\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/14_lMlxp4MvjsoIgGwghzmrw6m92viazQ.png?resize=519%2C349\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1J729DibfJEHvUwVm7BX7gjoRdXmlMlxp4MvjsoIgGwghzmrw6m92viazQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"520\" height=\"349 .94000244140625\" style=\"font-family: \u7b49\u7ebf;font-size: 16px;letter-spacing: 0.034em;\" width=\"519 .9400024414062\" data-recalc-dims=\"1\"><br \/><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\">p1 <- ggplot(pbmc, aes(Feat, Expr, color = Feat, fill = Feat)) + <\/span><\/code><code><span class=\"code-snippet_outer\"> geom_violin(scale = <span class=\"code-snippet__string\">\"width\"<\/span>, adjust = <span class=\"code-snippet__number\">1<\/span>, trim = <span class=\"code-snippet__keyword\">TRUE<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"> scale_y_continuous(expand = c(<span class=\"code-snippet__number\">0<\/span>, <span class=\"code-snippet__number\">0<\/span>),<\/span><\/code><code><span class=\"code-snippet_outer\"> position=<span class=\"code-snippet__string\">\"right\"<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> labels = <span class=\"code-snippet__function\"><span class=\"code-snippet__keyword\">function<\/span><span class=\"code-snippet__params\">(x)<\/span><\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">c<\/span><span class=\"code-snippet__params\">(rep<span class=\"code-snippet__params\">(x = <span class=\"code-snippet__string\">\"\"<\/span>, times = length<span class=\"code-snippet__params\">(x)<\/span><span class=\"code-snippet__number\">-2<\/span>)<\/span>, x[length<span class=\"code-snippet__params\">(x)<\/span> - <span class=\"code-snippet__number\">1<\/span>], <span class=\"code-snippet__string\">\"\"<\/span>)<\/span>) +<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">facet_grid<\/span><span class=\"code-snippet__params\">(rows = vars<span class=\"code-snippet__params\">(Idents)<\/span>, scales = <span class=\"code-snippet__string\">\"free\"<\/span>, switch = <span class=\"code-snippet__string\">\"y\"<\/span>)<\/span> +<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">theme_cowplot<\/span><span class=\"code-snippet__params\">(font_size = <span class=\"code-snippet__number\">12<\/span>)<\/span> +<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">theme<\/span><span class=\"code-snippet__params\">(legend.position = <span class=\"code-snippet__string\">\"none\"<\/span>,<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"> panel.spacing = unit<span class=\"code-snippet__params\">(<span class=\"code-snippet__number\">0<\/span>, <span class=\"code-snippet__string\">\"lines\"<\/span>)<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> plot.title = element_text<span class=\"code-snippet__params\">(hjust = <span class=\"code-snippet__number\">0.5<\/span>)<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> panel.background = element_rect<span class=\"code-snippet__params\">(fill = NA, color = <span class=\"code-snippet__string\">\"black\"<\/span>)<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> plot.margin = margin<span class=\"code-snippet__params\">(<span class=\"code-snippet__number\">6<\/span>, <span class=\"code-snippet__number\">6<\/span>, <span class=\"code-snippet__number\">0<\/span>, <span class=\"code-snippet__number\">6<\/span>, <span class=\"code-snippet__string\">\"pt\"<\/span>)<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> strip.background = element_blank<span class=\"code-snippet__params\">()<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> strip.text = element_text<span class=\"code-snippet__params\">(face = <span class=\"code-snippet__string\">\"bold\"<\/span>)<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> strip.text.y.left = element_text<span class=\"code-snippet__params\">(angle = <span class=\"code-snippet__number\">0<\/span>)<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.title.x = element_blank<span class=\"code-snippet__params\">()<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"> axis.ticks.x = element_blank<span class=\"code-snippet__params\">()<\/span>,<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet_outer\"> axis.text.x = element_blank<span class=\"code-snippet__params\">()<\/span>)<\/span> +<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">ggtitle<\/span><span class=\"code-snippet__params\">(<span class=\"code-snippet__string\">\"\"<\/span>)<\/span> + <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">ylab<\/span><span class=\"code-snippet__params\">(<span class=\"code-snippet__string\">\"Expression Level\"<\/span>)<\/span> +<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">scale_fill_manual<\/span><span class=\"code-snippet__params\">(values = spring)<\/span> +<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">scale_color_manual<\/span><span class=\"code-snippet__params\">(values = spring)<\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;\">\u548c\u4e0a\u8ff0\u4ee3\u7801\u4e00\u6837\uff0c\u5c0f\u4f19\u4f34\u591a\u591a\u7406\u89e3\u4e00\u4e0b<\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\">p1<\/span><\/p>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296899\" data-ratio=\"1.107750472589792\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/15_vq25ILYicsSBBx1KuVoT5RLF5saibg.png?resize=528%2C585\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1JtsKnmsw9juYEibEs5ykID9LH25Gvq25ILYicsSBBx1KuVoT5RLF5saibg\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"529\" height=\"585 .8800048828125\" width=\"528 .9400024414062\" data-recalc-dims=\"1\"><\/span><\/p>\n<p><span style=\"font-size: 16px;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u989c\u8272\u53ef\u80fd\u6ca1\u505a\u5230\u4e00\u6837\uff0c\u4f46\u662f\u4e5f\u633a\u597d\u770b\uff0c\u90a3\u4e48\u8fd9\u4e24\u4e2a\u56fe\u6211\u4eec\u90fd\u7ed8\u5236\u5b8c\u6210\u4e86\uff0c\u6211\u4eec\u5c31\u53bb\u7ec4\u5408\u4e00\u4e0b\u8fd9\u4e24\u4e2a\u56fe\uff0c\u6765\u770b\u770b\u6700\u7ec8\u7684\u6b65\u9aa4<\/span>    <\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"swift\"><code><span class=\"code-snippet_outer\">plot_grid(p1,p, align =<span class=\"code-snippet__string\">\"h\"<\/span>, axis=<span class=\"code-snippet__string\">\"bt\"<\/span>, rel_widths = <span class=\"code-snippet__built_in\">c<\/span>(<span class=\"code-snippet__number\">2<\/span>,<span class=\"code-snippet__number\">7<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\">rel_widths\u8fd9\u4e2a\u53ef\u4ee5\u8bbe\u7f6e\u4e24\u4e2a\u7ec4\u7ec4\u5408\u7684\u6bd4\u4f8b<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: \u7b49\u7ebf;font-variant: normal;text-transform: none;font-size: 16px;\"><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"503296901\" data-ratio=\"0.41388888888888886\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/16_cabcRxKCwk0tMPWxjskq50f5zKIfRA.png?resize=553%2C229\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/RpJ9AibwAxrEzPNiczNztpH1otVK9OGz1JJgPPWIm2UTdic3YFuv9fWBIdxcabcRxKCwk0tMPWxjskq50f5zKIfRA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"229 .07000732421875\" width=\"553 .739990234375\" data-recalc-dims=\"1\"><\/span><\/p>\n<p><span style=\"font-size: 16px;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u597d\u4e86\uff0c\u6211\u4eec\u7684\u7ec4\u5408\u56fe\u5c31\u7ed8\u5236\u5b8c\u6210\u4e86\u3002\u5bf9\u4e8e\u4e0a\u8ff0\u7684\u6570\u636e\uff0c\u5c0f\u4f19\u4f34\u53ef\u4ee5\u4f7f\u7528\u81ea\u5df1\u7684\u6570\u636e\u53bb\u8bbe\u7f6e\u4e00\u4e0b\uff0c\u8981\u591a\u591a\u7406\u89e3\u4ee3\u7801\u7684\u610f\u601d\uff0c\u5176\u5b9e\u8bb8\u591a\u9ad8\u5206\u6587\u7ae0\u4e2d\u7684\u56fe\u7247\u90fd\u662f\u4e00\u6b65\u4e00\u6b65\u7ec4\u5408\u800c\u6765\u7684\uff0c\u7406\u89e3\u4ee3\u7801\u7684\u610f\u601d\uff0c\u628a\u81ea\u5df1\u7684\u56fe\u7ed8\u5236\u9ad8\u7ea7\u8d77\u6765\uff0c\u5c0f\u4f19\u4f34\u5feb\u53bb\u52a8\u624b\u8bd5\u8bd5\u5427\uff01<\/span><span style=\"color: rgb(217, 33, 66);\"><strong><span style=\"font-size: 16px;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5982\u679c\u6709\u5176\u4ed6\u60f3\u8981\u590d\u73b0\u7684\u5185\u5bb9\u90fd\u53ef\u4ee5\u626b\u7801\u54a8\u8be2\u5c0f\u4e91\uff1b\u5c0f\u4e91\u4e0d\u53ea\u4f1a\u505a\u56fe\uff0c\u5f88\u591a\u9ad8\u5206\u6587\u732e\u601d\u8def\u90fd\u53ef\u4ee5\u8fdb\u884c\u590d\u73b0\u54e6\u3002<\/span><\/strong><\/span><span style=\"font-size: 16px;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\"><\/span><o:page><\/o:page><\/p>\n<p style=\"text-align: center;margin-bottom: 0px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"503296902\" data-ratio=\"0.3472222222222222\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/17_n5R2VzrWMh4W52J7CFN61kYce3rJWA.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/RpJ9AibwAxrE4LUhGSzbXXq9xT0gJjDV33AyNvkibuDtsMxH1ibVMcxIDzOn5R2VzrWMh4W52J7CFN61kYce3rJWA\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"\" data-recalc-dims=\"1\"><\/p>\n<section style=\"font-size: 16px;margin-bottom: 0px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: top;width: 50%;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 80%;vertical-align: top;box-shadow: rgb(204, 204, 204) 3.53553px 3.53553px 4px;border-style: solid;border-width: 2px;border-radius: 11px;border-color: rgb(114, 114, 114);overflow: hidden;align-self: flex-start;flex: 0 0 auto;\">\n<section style=\"padding: 10px;font-size: 13px;\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 15px;line-height: 1.3;\">\n<p><a target=\"_blank\" href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=MzAwNjE0MDY3MQ==&action=getalbum&album_id=3054525645025705990&token=1653828524&lang=zh_CN&scene=21#wechat_redirect\" textvalue=\"\u4e34\u5e8a\u6570\u636e\u6316\u6398\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" tab=\"innerlink\" data-linktype=\"2\" style=\"color: var(--weui-LINK);font-size: 15px;text-align: center;text-wrap: wrap;caret-color: rgba(0, 0, 0, 0);outline: 0px;cursor: pointer;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;letter-spacing: 0.544px;background-color: rgb(255, 255, 255);\" rel=\"noopener\">\u4e34\u5e8a\u6570\u636e\u6316\u6398<\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"text-align: justify;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><br \/><\/p>\n<\/section>\n<section style=\"text-align: center;justify-content: center;display: 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