{"id":60391,"date":"2024-06-17T11:20:55","date_gmt":"2024-06-17T03:20:55","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=60391"},"modified":"2024-06-17T11:20:55","modified_gmt":"2024-06-17T03:20:55","slug":"%e6%83%b3%e8%a6%81%e4%b8%80%e9%94%ae%e6%90%9e%e5%ae%9a%e6%89%80%e6%9c%89%e5%8d%95%e7%bb%86%e8%83%9e%e6%95%b0%e6%8d%ae%e7%9a%84%e6%89%b9%e6%ac%a1%e6%95%88%e5%ba%94%ef%bc%9f%e9%99%a4%e9%9d%9e%e4%bd%a0","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=60391","title":{"rendered":"\u60f3\u8981\u4e00\u952e\u641e\u5b9a\u6240\u6709\u5355\u7ec6\u80de\u6570\u636e\u7684\u6279\u6b21\u6548\u5e94\uff1f\u9664\u975e\u4f60\u7528Harmony"},"content":{"rendered":"


\n
\n<\/title><br \/>\n<meta charset=\"utf-8\"><br \/>\n<meta name=\"viewport\" content=\"width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0,viewport-fit=cover\"><\/p>\n<style>\n*{margin:0;padding:0}html{-ms-text-size-adjust:100%;-webkit-text-size-adjust:100%;line-height:1.6}img{z-index:999;position:relative;max-width:100%;margin:10px 0;}body{letter-spacing:.034em}h1,h2,h3,h4,h5,h6{font-weight:400;font-size:16px}a{color:#576b95;text-decoration:none;-webkit-tap-highlight-color:rgba(0,0,0,0)}td,th{word-wrap:break-word;padding:5px 10px;border:1px solid #DDD;}table{margin-bottom:10px;border-collapse:collapse;display:table;width:100%!important;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}.rich_media_area_primary{padding:20px 16px 12px;background-color:#fafafa}@media (max-width:375px){.rich_media_area_primary{padding:20px 60px 15px 60px}.rich_media_area_extra{padding:0 60px 21px 60px}}@media (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner,body{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media{padding:20px;overflow:hidden;}.appmsg_skin_default .rich_media_area_primary{background-color:#fff}.appmsg_skin_default .rich_media_area_primary .weui-loadmore_line .weui-loadmore__tips{background-color:#fff}@media screen and (min-width:1024px){.rich_media_area_primary_inner,.rich_media_area_extra_inner{max-width:677px;margin-left:auto;margin-right:auto}.rich_media_area_primary{padding-top:32px}}.rich_media_content{overflow:hidden;color:#333;font-size:17px;word-wrap:break-word;-webkit-hyphens:auto;-ms-hyphens:auto;hyphens:auto;text-align:justify;position:relative;z-index:0}.rich_media_content *{max-width:100%!important;box-sizing:border-box!important;-webkit-box-sizing:border-box!important;word-wrap:break-word!important}.rich_media_content p{clear:both;min-height:1em}.rich_media_content em{font-style:italic}.rich_media_content fieldset{min-width:0}.rich_media_content .list-paddingleft-1,.rich_media_content .list-paddingleft-2,.rich_media_content .list-paddingleft-3{padding-left:2.2em}.rich_media_content .list-paddingleft-1 .list-paddingleft-2,.rich_media_content .list-paddingleft-2 .list-paddingleft-2,.rich_media_content .list-paddingleft-3 .list-paddingleft-2{padding-left:30px}.rich_media_content .list-paddingleft-1{padding-left:1.2em}.rich_media_content .list-paddingleft-3{padding-left:3.2em}.rich_media_content .code-snippet,.rich_media_content .code-snippet__fix{max-width:1000%!important}.rich_media_content .code-snippet *,.rich_media_content .code-snippet__fix *{max-width:1000%!important}.rich_media_title{font-size:22px;line-height:1.4;margin-bottom:13px;padding-bottom:13px;border-bottom:1px solid #e7e7eb;}@supports(-webkit-overflow-scrolling:touch){.rich_media_title{font-weight:700}}.rich_media_meta{display:inline-block;vertical-align:middle;padding:0 0 10px 0;font-size:15px;-webkit-tap-highlight-color:rgba(0,0,0,0)}.rich_media_meta.icon_appmsg_tag{margin-right:0px}.rich_media_meta.meta_tag_text{margin-right:0}.rich_media_meta_list em{font-style:normal}.rich_media_meta_text{color:#a5a5a5;}p{margin:0;}.msgBox{margin-top:20px;padding-top:20px;padding-left:50px;overflow:hidden;border-top:2px dashed #09a2ff;}.msg{padding-top:7px;clear:both;}.msgBody{float:right;width:100%;margin-left:55px;padding-bottom:15px;border-bottom:1px dashed #e0e0e0;}.userHeadImg{float:left;margin-left:-50px;}.userHeadImg img{width:40px;height:40px;margin-right:10px;border-radius:3px;}.userName{color:#888888;line-height:24px;font-size:14px;margin:5px 0 5px 0;height:24px;}.replyBody,.autherBody{color:#565656;font-size:15px;}.replyIcon{border-left:4px solid #33ab01;margin-right:5px;}.ad{text-decoration:none;color:#d6d4d4;font-size:12px;}.msgBodyReply{padding-top:5px;}.userName span{float:right;color:#afafaf;font-size:14px;}code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;}.code-snippet__fix{font-size:14px;margin:10px 0;display:block;color:#333;position:relative;background-color:rgba(0,0,0,0.03);border:1px solid #f0f0f0;border-radius:2px;display:-webkit-box;display:-webkit-flex;display:flex;padding-left:25px;line-height:26px}.code-snippet__fix code{text-align:left;font-size:14px;display:block;white-space:pre;display:-webkit-box;display:-webkit-flex;display:flex;position:relative;font-family:Consolas,\"Liberation Mono\",Menlo,Courier,monospace}.code-snippet__comment,.code-snippet__quote{color:#afafaf;font-style:italic}.code-snippet__keyword,.code-snippet__selector-tag,.code-snippet__subst{color:#ca7d37}.code-snippet__number,.code-snippet__literal,.code-snippet__variable,.code-snippet__template-variable,.code-snippet__tag .code-snippet__attr{color:#0e9ce5}.code-snippet__string,.code-snippet__doctag{color:#d14}.code-snippet__title,.code-snippet__section,.code-snippet__selector-id{color:#d14}.code-snippet__subst{font-weight:normal}.code-snippet__type,.code-snippet__class .code-snippet__title{color:#0e9ce5}.code-snippet__tag,.code-snippet__name,.code-snippet__attribute{color:#0e9ce5;font-weight:normal}.code-snippet__regexp,.code-snippet__link{color:#ca7d37}.code-snippet__symbol,.code-snippet__bullet{color:#d14}.code-snippet__built_in,.code-snippet__builtin-name{color:#ca7d37}.code-snippet__meta{color:#afafaf}.code-snippet__deletion{background:#fdd}.code-snippet__addition{background:#dfd}.code-snippet__emphasis{font-style:italic}.code-snippet__strong{font-weight:bold}.account_avatar{width:40px;height:40px;padding:0;}.account_info{display:-webkit-box;display:-webkit-flex;display:flex;-webkit-box-align:center;-webkit-align-items:center;padding:20px 0;align-items:center}.flex_bd{padding-left:14px;}.account_nickname{display:inline-block;vertical-align:middle;line-height:1.2;color:#576b95;font-size:14px}.account_desc{overflow:hidden;text-overflow:ellipsis;display:-webkit-box;-webkit-box-orient:vertical;-webkit-line-clamp:1;color:rgba(0,0,0,0.3);font-size:14px;line-height:1.2;padding-top:.4em}.msg_source_url{text-align:left;word-break:break-all;margin-top:20px;}.msg_source_url a{padding-right:10px;}.msg_source_url .url_text{color:#a8a8a8;}.video-desc{font-size:14px;margin-top:15px;color:#6c6c6c;}.msg_source_url{text-align:left;}.original_primary_card_tips{color:rgba(0,0,0,0.3);line-height:1.4;font-size:15px;}.weui-flex__item{margin-bottom:20px;padding:20px 16px;margin-top:16px;line-height:1.4;align-items:center;background-color:#f7f7f7;border-radius:8px;position:relative;}.original_primary_desc{color:rgba(0,0,0,0.5);font-size:14px;padding-top:4px;width:auto;overflow:hidden;text-overflow:ellipsis;}.msgBodyReplyList{border-top:1px solid #e1e1e1;margin-top:10px;}.msgBodyReplyListTop{border-top:0;}.reply_like_num{float:right;font-size:14px;color:#c7c7c7;}.msgData{margin-top:20px;color:#626262;}.msgData span{font-size:14px;padding-right:15px;}.msgData .likes{float:right;padding-right:0;}.js_text_content p{font-size:18px;}.rich_media_meta_link{font-size:15px;}blockquote {padding-left: 10px;border-left: 3px solid #dbdbdb;color: rgba(0,0,0,0.5);font-size: 15px;padding-top: 4px;margin: 1em 0;}.video_iframe{width:500px;height:400px;}.blockquote_info{color:#b5b5b5;margin-top:10px;}#copyright_logo{color:#bdbdbd;}.rich_media_meta_list{margin-bottom:10px;}.reprint{background:#efefef;border-radius:5px;padding:8px;color:#1f1f1f;}.reprint a{word-break:break-all;}.topic{color:#8e8e8e;background:#f7f7f7;border-radius:5px;padding:10px 8px;}.topic a{padding-right:5px;}.topic p{margin-bottom:5px;}\n<\/style>\n<link href=\"https:\/\/www.juyifx.cn\/config\/css\/wxArticle.css\" rel=\"stylesheet\"\/>\n<\/head><script>\nvar data={\"mp\":\"\u751f\u4fe1\u679c\",\"title\":\"\u60f3\u8981\u4e00\u952e\u641e\u5b9a\u6240\u6709\u5355\u7ec6\u80de\u6570\u636e\u7684\u6279\u6b21\u6548\u5e94\uff1f\u9664\u975e\u4f60\u7528Harmony\",\"time\":\"2023-12-03 19:13:58\",\"timeStamp\":\"1701602038\"};\n<\/script><br \/>\n<body><\/p>\n<div class=\"rich_media\">\n<h1 class=\"rich_media_title\" id=\"activity-name\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247496554&idx=1&sn=38cfe823d9586324390dd86c65313603&chksm=cf7f63aff808eab9c39a9bfc48de143a62760993a3b04f7a6a23a2843ff8f7d8ef0a3cefc821#rd\" target=\"_blank\" rel=\"noopener\">\u60f3\u8981\u4e00\u952e\u641e\u5b9a\u6240\u6709\u5355\u7ec6\u80de\u6570\u636e\u7684\u6279\u6b21\u6548\u5e94\uff1f\u9664\u975e\u4f60\u7528Harmony<\/a><\/h1>\n<div id=\"meta_content\" class=\"rich_media_meta_list\"><span id=\"copyright_logo\" class=\"wx_tap_link js_wx_tap_highlight rich_media_meta icon_appmsg_tag appmsg_title_tag weui-wa-hotarea\">\u539f\u521b  <\/span><span class=\"rich_media_meta rich_media_meta_text\">\u5c0f\u679c  <\/span><span class=\"rich_media_meta rich_media_meta_nickname\" id=\"profileBt\"><a href=\"javascript:void(0);\" class=\"wx_tap_link js_wx_tap_highlight weui-wa-hotarea\" id=\"js_name\">\u751f\u4fe1\u679c  <\/a><\/span><em id=\"publish_time\" class=\"rich_media_meta rich_media_meta_text\">2023-12-03 19:13:58<\/em><\/div>\n<p><content><\/p>\n<div class=\"topic\">\n<p>\u6536\u5f55\u4e8e\u8bdd\u9898<\/p>\n<p><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=2664545260517294084#wechat_redirect\" title=\"\u751f\u4fe1\u5b9e\u64cd\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5b9e\u64cd<\/a><a href=\"https:\/\/mp.weixin.qq.com\/mp\/appmsgalbum?__biz=Mzg3OTg3MjQyNg==&action=getalbum&album_id=3138206666342268940#wechat_redirect\" title=\"\u751f\u4fe1\u5c0f\u767d\u76f8\u5173\" target=\"_blank\" rel=\"noopener\">#\u751f\u4fe1\u5c0f\u767d\u76f8\u5173<\/a><\/p>\n<\/div>\n<p data-mpa-powered-by=\"yiban.io\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100012439\" data-ratio=\"0.23333333333333334\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_rE2v0O5WiaUDJjgv5bxK2G1ePHyH2g-73.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAysHY49vPGCraibakiaNq6hQgn8C5MfvVLQGxlNZPEia2PajKAzrE2v0O5WiaUDJjgv5bxK2G1ePHyH2g\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"1080\" style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.578px;text-align: center;\" data-recalc-dims=\"1\" \/><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u6700\u8fd1\uff0c\u5c0f\u679c\u53d1\u73b0\u8bb8\u591a\u5c0f\u4f19\u4f34\u5728\u5904\u7406\u591a\u4e2a\u7ec4\u5b66\u6570\u636e\u96c6\u7684\u65f6\u5019\uff0c\u5e38\u5e38\u88ab\u6279\u6b21\u6548\u5e94\u56f0\u6270\uff0c\u8ba9\u4eba\u611f\u89c9\u5934\u5927\u65e0\u6bd4\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u522b\u62c5\u5fc3\uff0c\u5c0f\u679c\u4eca\u5929\u5c31\u6765\u4e3a\u4f60\u89e3\u56f0\uff01<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u6211\u8981\u4ecb\u7ecd\u7684\u662f\u4e00\u4e2a\u795e\u5947\u7684R\u5305\uff0c\u5b83\u7684\u540d\u5b57\u53eb\u505aHarmony\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u8fd9\u4e2aR\u5305\u4e13\u95e8\u7528\u4e8e\u5904\u7406\u548c\u6574\u5408\u591a\u4e2a\u7ec4\u5b66\u6570\u636e\u96c6\uff0c\u6781\u5927\u5730\u51cf\u5c0f\u4e86\u6279\u6b21\u6548\u5e94\u7684\u5f71\u54cd\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u4f7f\u7528\u5b83\uff0c\u4f60\u53ef\u4ee5\u81ea\u5982\u5730\u6574\u5408\u4e0d\u540c\u6765\u6e90\u3001\u4e0d\u540c\u6279\u6b21\u7684\u6570\u636e\uff0c\u8ba9\u4f60\u7684\u7814\u7a76\u66f4\u52a0\u6df1\u5165\u548c\u7cbe\u7ec6\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u4e0b\u9762\u5c31\u8ba9\u6211\u4eec\u5f00\u59cb\u5427\uff01<\/span><br \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u5728\u5f00\u59cb\u4e4b\u524d\uff0c\u5c0f\u679c\u5148\u79d1\u666e\u4e00\u4e0b\u6279\u6b21\u6548\u5e94\uff08\u4e5f\u79f0\u6280\u672f\u6df7\u6742\u56e0\u7d20\uff09\uff0c\u662f\u6307\u5728\u9ad8\u901a\u91cf\u751f\u7269\u6570\u636e\u5206\u6790\u4e2d\uff0c\u7531\u4e8e\u4e0d\u540c\u7684\u5b9e\u9a8c\u6761\u4ef6\u3001\u64cd\u4f5c\u8005\u3001\u5206\u79bb\u65b9\u6cd5\u3001\u8bd5\u5242\u3001\u4eea\u5668\u3001\u8fdb\u884c\u5b9e\u9a8c\u7684\u5b9e\u9a8c\u5ba4\u3001\u65f6\u95f4\u7b49\u56e0\u7d20\u5bfc\u81f4\u7684\u4e0e\u751f\u7269\u53d8\u5f02\u65e0\u5173\u7684\u6280\u672f\u5dee\u5f02\u3002\u6279\u6b21\u6548\u5e94\u4f1a\u5f71\u54cd\u6570\u636e\u7684\u8d28\u91cf\u548c\u53ef\u4fe1\u5ea6\uff0c\u5e72\u6270\u6570\u636e\u4e4b\u95f4\u7684\u771f\u5b9e\u5dee\u5f02\uff0c\u5bfc\u81f4\u5206\u6790\u7ed3\u679c\u7684\u504f\u5dee\u548c\u8bef\u5dee\u3002\u56e0\u6b64\uff0c\u6d88\u9664\u6216\u51cf\u5c11\u6279\u6b21\u6548\u5e94\u662f\u6570\u636e\u5206\u6790\u7684\u4e00\u4e2a\u91cd\u8981\u6b65\u9aa4\u3002<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">Harmony\u5de5\u5177\u5305\u53bb\u6279\u6b21\u6548\u5e94\u7684\u539f\u7406\uff1a<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">Harmony \u662f\u4e00\u79cd\u7528\u4e8e\u5355\u7ec6\u80de RNA-\u6d4b\u5e8f\u6570\u636e (scRNA-seq) \u7684\u6279\u6b21\u6548\u5e94\u4fee\u6b63\u65b9\u6cd5\u3002\u7531\u4e8e\u5b83\u662f\u5728\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u4e2d\u4f7f\u7528\u7684\uff0c\u5c0f\u679c\u7b80\u8981\u6982\u62ec\u4e00\u4e0b\u5b83\u7684\u5de5\u4f5c\u539f\u7406\u3002<\/span><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012441\" data-ratio=\"0.4648148148148148\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_AK0icvZEOEHlWI2icBuXkOVUicnDzw.png?resize=553%2C257\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPqeNQsFjm2HibIuSld9Nh9HBVNmNPFqe3dREOiaH0R7HruK1LKAK0icvZEOEHlWI2icBuXkOVUicnDzw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"257 .1400146484375\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u2022       \u9996\u5148\uff0c\u4f7f\u7528\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u5c06\u7ec6\u80de\u5d4c\u5165\u5230\u4f4e\u7ef4\u7a7a\u95f4\u4e2d\u3002   <\/span><o:page><\/o:page><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u2022       \u7136\u540e\uff0c\u4f7f\u7528\u6a21\u7cca\u805a\u7c7b\u5c06\u6bcf\u4e2a\u7ec6\u80de\u5206\u914d\u5230\u591a\u4e2a\u805a\u7c7b\u4e2d\uff0c\u540c\u65f6\u4f7f\u7528\u4e00\u4e2a\u60e9\u7f5a\u9879\u6765\u6700\u5927\u5316\u6bcf\u4e2a\u805a\u7c7b\u5185\u6570\u636e\u96c6\u7684\u591a\u6837\u6027\u3002<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u2022       \u63a5\u7740\uff0c\u8ba1\u7b97\u6bcf\u4e2a\u805a\u7c7b\u7684\u5168\u5c40\u8d28\u5fc3\uff0c\u4ee5\u53ca\u6bcf\u4e2a\u805a\u7c7b\u7684\u6570\u636e\u96c6\u7279\u5b9a\u8d28\u5fc3\u3002<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u2022       \u7136\u540e\uff0c\u6839\u636e\u8d28\u5fc3\u8ba1\u7b97\u6bcf\u4e2a\u6570\u636e\u96c6\u7684\u6821\u6b63\u56e0\u5b50\u3002<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u2022       \u6700\u540e\uff0c\u4f7f\u7528\u7279\u5b9a\u4e8e\u7ec6\u80de\u7684\u56e0\u5b50\u6821\u6b63\u6bcf\u4e2a\u7ec6\u80de\uff1a\u7531\u5728\u6a21\u7cca\u805a\u7c7b\u4e2d\u8fdb\u884c\u7684\u8f6f\u5206\u914d\u52a0\u6743\u7684\u6570\u636e\u96c6\u6821\u6b63\u56e0\u5b50\u7684\u7ebf\u6027\u7ec4\u5408\u3002<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u91cd\u590d\u4e0a\u8ff0\u6b65\u9aa4\u76f4\u5230\u6536\u655b\u3002<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u6240\u4ee5\uff0cHarmony \u7684\u5de5\u4f5c\u539f\u7406\u5c31\u662f\u901a\u8fc7\u627e\u51fa\u4e0d\u540c\u7ec6\u80de\u6279\u6b21\u4e4b\u95f4\u7684\u76f8\u4f3c\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\uff0c\u5e76\u6839\u636e\u8fd9\u4e9b\u6a21\u5f0f\u8c03\u6574\u5b83\u4eec\u7684\u6570\u636e\uff0c\u4ece\u800c\u6d88\u9664\u6279\u6b21\u6548\u5e94\u3002\u60f3\u6df1\u5165\u4e86\u89e3\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u53bb\u770b\u539f\u6587\uff1a<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">https:\/\/www.nature.com\/articles\/s41592-019-0619-0<\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012440\" data-ratio=\"0.38425925925925924\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_C0b7ZibwSiaLrgfF7DhzOA5ZCQJSBA.png?resize=553%2C212\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPqeNQsFjm2HibIuSld9Nh9rHMV9wcIxdm6BJafNVibSkCrISUC0b7ZibwSiaLrgfF7DhzOA5ZCQJSBA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"212 .66998291015625\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"letter-spacing: 0.034em;font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"><\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u9996\u5148\uff0c\u8ba9\u6211\u4eec\u6765\u4e86\u89e3\u4e00\u4e0bHarmony\u5305\u7684\u5b89\u88c5\u6b65\u9aa4\u3002\u5728R\u73af\u5883\u4e2d\uff0c\u60a8\u53ef\u4ee5\u4f7f\u7528\u4ee5\u4e0b\u4ee3\u7801\u5b89\u88c5\u8be5\u5305\uff1a<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100012444\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_IWicymEetCc6Enf2BkUE4Yz1Ya5e5w-8.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyuYsqlJnQUJvnS2iciaDibtfbdV0VmHlriaSjYwjFPLp1BLjokHIWicymEetCc6Enf2BkUE4Yz1Ya5e5w\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"letter-spacing: 0.578px;text-wrap: wrap;text-align: center;\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\"> \u5982\u679c\u60a8\u5c1a\u672a\u5b89\u88c5BiocManager\uff0c\u8bf7\u5148\u5b89\u88c5<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">if<\/span> (!requireNamespace(<span class=\"code-snippet__string\">\"BiocManager\"<\/span>, quietly = <span class=\"code-snippet__keyword\">TRUE<\/span>))<\/span><\/code><code><span class=\"code-snippet_outer\"> install.packages(<span class=\"code-snippet__string\">\"BiocManager\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\"># \u5b89\u88c5Harmony\u5305<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">BiocManager::install(<span class=\"code-snippet__string\">\"Harmony\"<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u5c0fTip<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u5047\u5982\u8fd8\u5b89\u88c5\u4e0d\u4e0a\uff0c\u4e5f\u53ef\u4ee5\u7528\u5c0f\u679c\u4e4b\u524d\u4ecb\u7ecd\u7684devtools\u4e00\u952e\u5b89\u88c5<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"javascript\"><code><span class=\"code-snippet_outer\">library(devtools)<\/span><\/code><code><span class=\"code-snippet_outer\">install_github(<span class=\"code-snippet__string\">\"immunogenomics\/harmony\"<\/span>) <\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u5b89\u88c5\u5b8c\u6210\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u5f00\u59cb\u4f7f\u7528Harmony\u5305\u6765\u8c03\u6574\u6279\u6b21\u6548\u5e94\u3002<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u5b9e\u6218\u6f14\u7ec3\uff1a<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u4e0b\u8f7d\u793a\u4f8b\u6570\u636e\uff08\u5c0f\u679c\u5df2\u7ecf\u5e2e\u4f60\u627e\u597d\u4e86<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u767e\u5ea6\u4e91\u94fe\u63a5\uff1a<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">https:\/\/pan.baidu.com\/s\/1BcimNtpmfYPCXAzJDaBaUQ<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u63d0\u53d6\u7801\uff1alnfy<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"># \u5bfc\u5165\u6570\u636e, \u662f\u4e24\u4e2a\u7a00\u758f\u77e9\u9635<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"sql\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">load<\/span>(<span class=\"code-snippet__string\">'pbmc_test.RData'<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\"># \u5bfc\u5165\u6240\u9700<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u7684\u5e93<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\"><code><span class=\"code-snippet_outer\">library(harmony)<\/span><\/code><code><span class=\"code-snippet_outer\">library(Seurat)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">#\u521b\u5efa\u4e00\u4e2aSeurat\u5bf9\u8c61\uff0c\u7528\u4e8e\u5b58\u50a8\u548c\u5206\u6790\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">counts\u53c2\u6570\u662f\u6307\u5b9a\u8ba1\u6570\u77e9\u9635\uff0c\u8fd9\u91cc\u662f\u5c06\u4e24\u4e2a\u7a00\u758f\u77e9\u9635\uff08\u5206\u522b\u4ee3\u8868\u523a\u6fc0\u7ec4\u548c\u5bf9\u7167\u7ec4\u7684\u5355\u7ec6\u80de\u6570\u636e\uff09\u6309\u5217\u5408\u5e76\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">min.cells=5\uff0c\u8868\u793a\u53ea\u4fdd\u7559\u5728\u81f3\u5c115\u4e2a\u7ec6\u80de\u4e2d\u6709\u8868\u8fbe\u7684\u57fa\u56e0\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\">pbmc <span class=\"code-snippet__tag\"><<span class=\"code-snippet__name\">-<\/span> <span class=\"code-snippet__attr\">CreateSeuratObject<\/span>(<span class=\"code-snippet__attr\">counts<\/span> = <span class=\"code-snippet__string\">cbind(stim.sparse,<\/span> <span class=\"code-snippet__attr\">ctrl.sparse<\/span>), <span class=\"code-snippet__attr\">project<\/span> = <span class=\"code-snippet__string\">\"PBMC\"<\/span>, <span class=\"code-snippet__attr\">min.cells<\/span> = <span class=\"code-snippet__string\">5)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">#\u7ed9Seurat\u5bf9\u8c61\u6dfb\u52a0\u65b0\u5217\uff0c\u5c06\u6bcf\u4e2a\u7ec6\u80de\u5206\u4e3a\u523a\u6fc0\u7ec4\u6216\u662f\u5bf9\u7167\u7ec4\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">pbmc<\/span>@<span class=\"code-snippet__keyword\">meta<\/span>.<span class=\"code-snippet__keyword\">data<\/span>$<span class=\"code-snippet__keyword\">group<\/span> <- c(rep(<span class=\"code-snippet__string\">\"STIM\"<\/span>, ncol(stim.sparse)), rep(<span class=\"code-snippet__string\">\"CTRL\"<\/span>, ncol(ctrl.sparse)))<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">#\u5bf9\u77e9\u9635\u8fdb\u884c\u5f52\u4e00\u5316\u5904\u7406\uff0c\u4f7f\u6bcf\u4e2a\u7ec6\u80de\u7684\u57fa\u56e0\u8868\u8fbe\u91cf\u53ef\u4ee5\u5728\u4e0d<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u540c\u7ec6\u80de\u4e4b\u95f4\u8fdb\u884c\u6bd4\u8f83\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">verbose\u53c2\u6570\u6307\u662f\u5426\u6253\u5370\u51fa\u8be6\u7ec6\u4fe1\u606f\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"php\"><code><span class=\"code-snippet_outer\">pbmc <- Seurat::NormalizeData(pbmc,verbose = <span class=\"code-snippet__keyword\">FALSE<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">#\u627e\u51fa\u4e0d\u540c\u7ec6\u80de\u4e4b\u95f4\u6709\u663e\u8457\u5dee\u5f02\u7684\u57fa\u56e0\u3002\uff0c\u7528\u4e8e\u540e\u7eed\u7684\u805a\u7c7b\u548c\u964d\u7ef4\u5206\u6790\u3002selection.method\u53c2\u6570\u662f\u6307\u5b9a\u9009\u62e9\u65b9\u6cd5\uff0c\u8fd9\u91cc\u662f”vst”\uff0c\u610f\u601d\u662f\u4f7f\u7528\u65b9\u5dee\u7a33\u5b9a\u5316\u53d8\u6362\uff08variance stabilizing transformation\uff09\u3002nfeatures\u53c2\u6570\u662f\u6307\u5b9a\u8981\u9009\u62e9\u7684\u7279\u5f81\u57fa\u56e0\u6570\u76ee\uff0c\u8fd9\u91cc\u662f2000\u3002   <\/span><o:page><\/o:page><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\">pbmc <span class=\"code-snippet__tag\"><<span class=\"code-snippet__name\">-<\/span> <span class=\"code-snippet__attr\">FindVariableFeatures<\/span>(<span class=\"code-snippet__attr\">pbmc<\/span>, <span class=\"code-snippet__attr\">selection.method<\/span> = <span class=\"code-snippet__string\">\"vst\"<\/span>, <span class=\"code-snippet__attr\">nfeatures<\/span> = <span class=\"code-snippet__string\">2000)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">#\u5c06\u6240\u6709\u57fa\u56e0<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u7684\u540d\u79f0\u8d4b\u503c\u7ed9\u4e00\u4e2a\u53d8\u91cf<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"css\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__selector-tag\">all<\/span><span class=\"code-snippet__selector-class\">.genes<\/span> <<span class=\"code-snippet__selector-tag\">-<\/span> <span class=\"code-snippet__selector-tag\">rownames<\/span>(<span class=\"code-snippet__selector-tag\">pbmc<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">#\u5bf9\u53d8\u5f02\u7279\u5f81\u57fa\u56e0\u8fdb\u884c\u6807\u51c6\u5316\u5904\u7406\uff0c\u4f7f\u6bcf\u4e2a\u57fa\u56e0\u7684\u8868\u8fbe\u91cf\u6709\u4e00\u4e2a\u5747\u503c\u4e3a0\uff0c\u6807\u51c6\u5dee\u4e3a1\u7684\u6b63\u6001\u5206\u5e03\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">\u8fd9\u6837\u53ef\u4ee5\u6d88\u9664\u4e0d\u540c\u57fa\u56e0\u4e4b\u95f4\u7684\u91cf\u7eb2\u5dee\u5f02\uff0c\u4e5f\u53ef\u4ee5\u51cf\u5c11\u5f02\u5e38\u503c\u7684\u5f71\u54cd\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">features\u53c2\u6570\u662f\u6307\u5b9a\u8981\u8fdb\u884c\u6807\u51c6\u5316\u7684\u57fa\u56e0\u5217\u8868\uff0c\u8fd9\u91cc\u662f\u4e4b\u524d\u5b58\u50a8\u7684\u6240\u6709\u57fa\u56e0\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"apache\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attribute\">pbmc<\/span> <- ScaleData(pbmc, features = <span class=\"code-snippet__literal\">all<\/span>.genes)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">#\u964d\u7ef4\u805a\u7c7b<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">#\u4f7f\u7528\u4e3b\u6210\u5206\u5206\u6790\uff08principal component analysis\uff09\u5c06\u9ad8\u7ef4\u7684\u6570\u636e\u6295\u5f71\u5230\u4f4e\u7ef4\u7684\u7a7a\u95f4\u4e2d\uff0c\u5f62\u6210\u4e00\u7ec4\u4e92\u76f8\u6b63\u4ea4\u7684\u4e3b\u6210\u5206\uff08principal components\uff09\uff0c\u6bcf\u4e2a\u4e3b\u6210\u5206\u90fd\u4ee3\u8868\u4e86\u6570\u636e\u4e2d\u7684\u4e00\u4e2a\u65b9\u5dee\u6700\u5927\u7684\u65b9\u5411\u3002npcs\u53c2\u6570\u662f\u6307\u5b9a\u8981\u4fdd\u7559\u7684\u4e3b\u6210\u5206\u6570\u76ee\uff0c\u8fd9\u91cc\u662f30\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\">pbmc <span class=\"code-snippet__tag\"><<span class=\"code-snippet__name\">-<\/span> <span class=\"code-snippet__attr\">RunPCA<\/span>(<span class=\"code-snippet__attr\">pbmc<\/span>, <span class=\"code-snippet__attr\">npcs<\/span> = <span class=\"code-snippet__string\">50)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\"># \u6839\u636e\u4e3b\u6210\u5206\u8ba1\u7b97\u6bcf\u4e2a\u7ec6\u80de\u4e0e\u5176\u4ed6\u7ec6\u80de\u4e4b\u95f4\u7684\u76f8\u4f3c\u5ea6\uff0c\u5e76\u6784\u5efa\u4e00\u4e2a\u90bb\u63a5\u77e9\u9635\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">dims\u53c2\u6570\u662f\u6307\u5b9a\u8981\u4f7f\u7528\u7684\u4e3b\u6210\u5206\u8303\u56f4\uff0c\u8fd9\u91cc\u662f1\u523030\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\">pbmc <span class=\"code-snippet__tag\"><<span class=\"code-snippet__name\">-<\/span> <span class=\"code-snippet__attr\">FindNeighbors<\/span>(<span class=\"code-snippet__attr\">pbmc<\/span>, <span class=\"code-snippet__attr\">dims<\/span> = <span class=\"code-snippet__string\">1:30)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\"># \u6839\u636e\u90bb\u63a5\u77e9\u9635\u5bf9\u7ec6\u80de\u8fdb\u884c\u805a\u7c7b\uff0c\u4e5f\u5c31\u662f\u5c06\u76f8\u4f3c\u5ea6\u9ad8\u7684\u7ec6\u80de\u5212\u5206\u4e3a\u4e00\u4e2a\u7fa4\u4f53\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\">resolution\u53c2\u6570\u662f\u6307\u5b9a\u805a\u7c7b\u7684\u7cbe\u5ea6\uff0c\u4e5f\u5c31\u662f\u51b3\u5b9a\u805a\u7c7b\u4e2a\u6570\u7684\u53c2\u6570\uff0c\u6570\u503c\u8d8a\u5927\u805a\u7c7b\u4e2a\u6570\u8d8a\u591a\u3002<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\">pbmc <span class=\"code-snippet__tag\"><<span class=\"code-snippet__name\">-<\/span> <span class=\"code-snippet__attr\">FindClusters<\/span>(<span class=\"code-snippet__attr\">pbmc<\/span>, <span class=\"code-snippet__attr\">resolution<\/span> = <span class=\"code-snippet__string\">0.3)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"># \u5bf9\u805a\u7c7b\u540e\u7684\u6570\u636e\u8fdb\u884cUMAP\uff08uniform manifold approximation and projection\uff09\u4ee5\u4fbf\u4e8e\u5728\u4e8c\u7ef4\u6216\u4e09\u7ef4\u7a7a\u95f4\u4e2d\u53ef\u89c6\u5316\u7ec6\u80de\u7fa4\u4f53\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"xml\"><code><span class=\"code-snippet_outer\">pbmc <span class=\"code-snippet__tag\"><<span class=\"code-snippet__name\">-<\/span> <span class=\"code-snippet__attr\">RunUMAP<\/span>(<span class=\"code-snippet__attr\">pbmc<\/span>, <span class=\"code-snippet__attr\">dims<\/span> = <span class=\"code-snippet__string\">1:30)<\/span><\/span><\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"># \u6309\u5206\u7ec4\u548c\u805a\u7c7b\u6570\u76ee\u7ed8\u5236\u7ec4\u522bumap\u56fe    <\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\">p1 <- DimPlot(pbmc, reduction = <span class=\"code-snippet__string\">\"umap\"<\/span>,<span class=\"code-snippet__keyword\">group<\/span>.<span class=\"code-snippet__keyword\">by<\/span> = <span class=\"code-snippet__string\">\"group\"<\/span>, pt.size=<span class=\"code-snippet__number\">0.5<\/span>, label = F,repel = TRUE)<\/span><\/code><code><span class=\"code-snippet_outer\">p2 <- DimPlot(pbmc, reduction = <span class=\"code-snippet__string\">\"umap\"<\/span>,<span class=\"code-snippet__keyword\">group<\/span>.<span class=\"code-snippet__keyword\">by<\/span> = <span class=\"code-snippet__string\">\"seurat_clusters\"<\/span>, pt.size=<span class=\"code-snippet__number\">0.5<\/span>, label = F,repel = TRUE)<\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012438\" data-ratio=\"0.5\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_e2a7poVwMFWicDcfVxPku9J5RBPDZA.png?resize=393%2C196\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPqeNQsFjm2HibIuSld9Nh95wRsnZzA7XUvus89Em2tblFFIe2a7poVwMFWicDcfVxPku9J5RBPDZA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"902\" height=\"196 .66998291015625\" style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;\" width=\"393 .3399658203125\" data-recalc-dims=\"1\" \/><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u53ef\u4ee5\u53d1\u73b0\u672a\u7ecf\u6821\u6b63\u7684\u6570\u636e\u96c6\u4e4b\u95f4\u5b58\u5728\u660e\u663e\u5dee\u5f02<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"># \u4f7f\u7528RunHarmony\u51fd\u6570\u53bb\u6279\u6b21<\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">#\u6309\u7167\u6837\u672c\u7ec4\u522b\u53bb\u6279\u6b21\uff0c\u8fd9\u91cc\u4f7f\u7528\u4e86\u7ba1\u9053\u51fd\u6570%>%\uff0c\u5373\u628a\u51fd\u6570\u5de6\u8fb9\u7684\u503c\u53d1\u7ed9\u53f3\u8fb9\u505a\u540e\u7eed\u5904\u7406\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"ini\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__attr\">pbmc<\/span> = pbmc %>% RunHarmony(<span class=\"code-snippet__string\">\"group\"<\/span>, plot_convergence = <span class=\"code-snippet__literal\">TRUE<\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012437\" data-ratio=\"0.877030162412993\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_K00iaXY6ibytm8icpXbc7icbj0JzOw.png?resize=206%2C180\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPqeNQsFjm2HibIuSld9Nh92VwCHrFL0m7N1fJ97ia2kuUtIALrvK00iaXY6ibytm8icpXbc7icbj0JzOw\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"431\" height=\"180 .66998291015625\" width=\"206 .0\" data-recalc-dims=\"1\" \/><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012442\" data-ratio=\"0.9915611814345991\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_9QsJGwAyvPwQYcmDSxlxKHib4EQaOQ.png?resize=250%2C248\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPqeNQsFjm2HibIuSld9Nh9DZXTiakeuJkRofBI40Nsrf4kLC9QsJGwAyvPwQYcmDSxlxKHib4EQaOQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"474\" height=\"248 .66998291015625\" width=\"250 .79000854492188\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">Harmony\u7b97\u6cd5\u5728\u8fdb\u884c\u4e866\u6b21\u8fed\u4ee3\u540e\u6536\u655b\uff0c\u6b64\u65f6\u5b83\u627e\u5230\u4e86\u4e00\u4e2a\u6700\u4f18\u7684\u89e3\u51b3\u65b9\u6848\uff0c\u4f7f\u5f97\u6570\u636e\u96c6\u4e4b\u95f4\u7684\u5dee\u5f02\u6700\u5c0f\u5316\uff0c\u800c\u7ec6\u80de\u7c7b\u578b\u4e4b\u95f4\u7684\u5dee\u5f02\u6700\u5927\u5316\u3002<\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"cs\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\">#\u91cd\u590d\u6807\u51c6\u6d41\u7a0b\uff0c\u53c2\u6570\u4e0d\u53d8<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pbmc2 <- pbmc2 %>%<\/span><\/code><code><span class=\"code-snippet_outer\"> RunUMAP(reduction = <span class=\"code-snippet__string\">\"harmony\"<\/span>, dims = <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">30<\/span>) %>%<\/span><\/code><code><span class=\"code-snippet_outer\"> FindNeighbors(reduction = <span class=\"code-snippet__string\">\"harmony\"<\/span>, dims = <span class=\"code-snippet__number\">1<\/span>:<span class=\"code-snippet__number\">30<\/span>) %>% <\/span><\/code><code><span class=\"code-snippet_outer\"> FindClusters(resolution = <span class=\"code-snippet__number\">0.3<\/span>) %>%<\/span><\/code><code><span class=\"code-snippet_outer\"> identity()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__meta\"># \u67e5\u770b\u6821\u6b63\u540e\u7684\u7ed3\u679c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">p3 <- DimPlot(pbmc, reduction = <span class=\"code-snippet__string\">\"umap\"<\/span>,<span class=\"code-snippet__keyword\">group<\/span>.<span class=\"code-snippet__keyword\">by<\/span> = <span class=\"code-snippet__string\">\"group\"<\/span>, pt.size=<span class=\"code-snippet__number\">0.5<\/span>, label = F,repel = TRUE)<\/span><\/code><code><span class=\"code-snippet_outer\">p4 <- DimPlot(pbmc, reduction = <span class=\"code-snippet__string\">\"umap\"<\/span>,<span class=\"code-snippet__keyword\">group<\/span>.<span class=\"code-snippet__keyword\">by<\/span> = <span class=\"code-snippet__string\">\"seurat_clusters\"<\/span>, pt.size=<span class=\"code-snippet__number\">0.5<\/span>, label = F,repel = TRUE)<\/span><\/code><\/pre>\n<\/section>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\"><\/span><img decoding=\"async\" loading=\"lazy\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012443\" data-ratio=\"0.5\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_cQPBD3dLgicrDIFsDZicBOwGE5SJxA.png?resize=553%2C276\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxPqeNQsFjm2HibIuSld9Nh9s2bILcPibM6rbx02GwdY75trhYOcQPBD3dLgicrDIFsDZicBOwGE5SJxA\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" height=\"276 .8699951171875\" width=\"553 .739990234375\" data-recalc-dims=\"1\" \/><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">#\u53bb\u6279\u6b21\u540e\u6837\u672c\u5206\u5e03\u7684\u6bd4\u8f83\u4e00\u81f4<\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;letter-spacing: 0.034em;\"><\/span><\/p>\n<p><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;\">\u770b\uff0c\u662f\u4e0d\u662f\u975e\u5e38\u7b80\u5355\uff1fHarmony\u5305\u5c31\u662f\u8fd9\u4e48\u795e\u5947\uff0c\u53ef\u4ee5\u8ba9\u4f60\u8f7b\u677e\u5904\u7406\u548c\u6574\u5408\u591a\u4e2a\u7ec4\u5b66\u6570\u636e\u96c6\uff0c\u8ba9\u4f60\u7684\u7814\u7a76\u66f4\u52a0\u987a\u5229\u3002\u4f46\u662f\uff0c\u8bf7\u8bb0\u4f4f\uff0c\u8fd9\u53ea\u662f\u4e00\u4e2a\u57fa\u7840\u7684\u4f7f\u7528\u6559\u7a0b\uff0cHarmony\u5305\u7684\u5f3a\u5927\u529f\u80fd\u8fd8\u6709\u5f88\u591a\u7b49\u7740\u4f60\u53bb\u53d1\u6398\u3002\u6240\u4ee5\uff0c\u5c0f\u4f19\u4f34\u4eec\uff0c\u5982\u679c\u4f60\u4eec\u624b\u5934\u6709\u591a\u4e2a\u7ec4\u5b66\u6570\u636e\u96c6\u9700\u8981\u5904\u7406\uff0c\u90a3\u5c31\u8d76\u5feb\u52a8\u624b\u8bd5\u8bd5\u5427\uff01<\/span><\/p>\n<section powered-by=\"xiumi.us\" style=\"margin-bottom: 0px;letter-spacing: 0.578px;text-wrap: wrap;outline: 0px;font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;background-color: rgb(255, 255, 255);text-align: left;\">\n<section style=\"outline: 0px;font-size: 16px;\">\n<section powered-by=\"xiumi.us\" style=\"margin: 10px;outline: 0px;justify-content: flex-start;display: flex;flex-flow: row;\">\n<section style=\"padding-right: 10px;padding-left: 10px;outline: 0px;display: inline-block;width: 558px;vertical-align: top;border-style: dashed;border-width: 1px;border-color: rgb(62, 62, 62);align-self: flex-start;flex: 0 0 auto;\">\n<section powered-by=\"xiumi.us\" style=\"margin-top: 10px;margin-bottom: 10px;outline: 0px;\">\n<section style=\"outline: 0px;display: inline-block;width: 536px;border-width: 0px 0px 0px 10px;border-style: solid;border-left-color: rgb(144, 215, 236);border-right-color: rgb(46, 172, 109);box-shadow: rgb(0, 0, 0) 0px 0px 0px;border-bottom-left-radius: 0px;\">\n<section powered-by=\"xiumi.us\" style=\"padding-right: 3px;padding-left: 3px;outline: 0px;\">\n<section style=\"margin-top: 0.95em;margin-bottom: -0.65em;outline: 0px;display: inline-block;width: 520px;vertical-align: top;\">\n<section style=\"margin-top: -1em;padding: 3px 10px;outline: 0px;display: inline-block;vertical-align: top;color: rgb(0, 154, 68);\">\n<p style=\"outline: 0px;\"><strong style=\"outline: 0px;\">\u5f80\u671f\u63a8\u8350<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section powered-by=\"xiumi.us\" style=\"outline: 0px;text-align: justify;\">\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491794&idx=1&sn=2b7a4de95169c79202e44813885a8250&chksm=cf7f7017f808f9015689839fe4057fd418bf7b20eb1dfb5a28cd92e56a41b5605e62bfbc16fb&scene=21#wechat_redirect\" textvalue=\"1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" rel=\"noopener\">1.\u642d\u5efa\u751f\u4fe1\u5206\u6790\u6d41\u6c34\u7ebf\uff0c\u5982\u5de5\u5382\u4e00\u683724\u5c0f\u65f6\u8fd0\u8f6cSnakemake\u2014\u2014\u8fdb\u9636\u547d\u4ee4<\/a><br style=\"outline: 0px;\" \/><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491705&idx=1&sn=9ff9b6da28b3a256a99c6697d589be66&chksm=cf7f70bcf808f9aafd1019a0bba3dd3a55e2867dbb9a09203f13c2683adf867094637c405ed1&scene=21#wechat_redirect\" textvalue=\"2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" rel=\"noopener\">2.\u6bd4blast\u8fd8\u4f18\u79c0\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff1fHMMER\u6765\u4e86<\/a><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491909&idx=1&sn=902e34bcf53e605fba2b457ec6a33e36&chksm=cf7f7180f808f8961542f458a49da14e85d8831449fb65fbf2623d55affc564686a5422f56fd&scene=21#wechat_redirect\" textvalue=\"3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;font-size: 14px;\" rel=\"noopener\">3.\u5bf9\u5355\u7ec6\u80de\u5206\u6790\u6beb\u65e0\u5934\u7eea\uff1f\u8ba9popsicleR\u9886\u4f60\u5165\u95e8<\/a><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247491905&idx=1&sn=fb34ebd6282962c7803badaee7a39096&chksm=cf7f7184f808f8929608c0050344ad86193d0fc6455c1c18e16cc17bf4b37832b63d3b18284e&scene=21#wechat_redirect\" textvalue=\"4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;\" rel=\"noopener\"><span style=\"outline: 0px;font-size: 14px;\">4.\u5c0f\u679c\u5e26\u4f60\u7ed8\u5236ROC\u66f2\u7ebf\u8bc4\u4f30\u751f\u5b58\u9884\u6d4b\u80fd\u529b<\/span><\/a><\/section>\n<section style=\"outline: 0px;letter-spacing: 0.578px;line-height: 1.75em;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247493633&idx=1&sn=2263dfe7572c0bcf6816c2c1a37d79de&chksm=cf7f78c4f808f1d2209321d6ace39fde5fa60c0691b56f88eac21916f5a2872ce8ee345f1d5f&scene=21#wechat_redirect\" textvalue=\"\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" hasload=\"1\" style=\"color: var(--weui-LINK);outline: 0px;cursor: pointer;letter-spacing: 0.578px;font-size: 14px;\" rel=\"noopener\">5.\u8f6f\u4ef6\u5305\u5b89\u88c5\u3001\u6253\u602a\u5feb\u53c8\u597d\uff0c1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff1b\u8fd8\u6709\u6bd4\u8fd9\u66f4\u7701\u94b1\u7684\u561b\uff01\uff01\uff01<\/a><span style=\"letter-spacing: 0.578px;text-align: center;font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;font-size: var(--articleFontsize);\"><\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"margin-top: 0pt;margin-bottom: 0pt;margin-left: 0pt;letter-spacing: 0.578px;text-indent: 0pt;text-wrap: wrap;text-align: left;line-height: normal;font-size: 12pt;font-family: helvetica;color: rgb(0, 0, 0);break-after: auto;\"><span style=\"font-family: \u5b8b\u4f53;font-size: 12pt;text-indent: 0pt;color: rgba(0, 0, 0, 0.9);letter-spacing: 0.034em;\"><\/span><span style=\"font-family: "Helvetica Neue", Helvetica, "Hiragino Sans GB", "Microsoft YaHei", Arial, sans-serif;font-size: 15px;color: rgba(0, 0, 0, 0.9);letter-spacing: 0.034em;text-align: justify;\"><\/span><span style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;color: rgba(0, 0, 0, 0.9);font-family: mp-quote, -apple-system-font, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif;text-align: justify;\"><\/span><span style=\"font-size: 15px;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100012455\" data-ratio=\"0.38981481481481484\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_FbEg9fibwQicL8l2ExKOZZTmAHXabQ-10.png?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAx3HetBkB7aOGibFWKqibqIATyC4zFibzCgdrxFwRep6cDMLIbA0FbEg9fibwQicL8l2ExKOZZTmAHXabQ\/640?wx_fmt=png'\" data-type=\"png\" data-w=\"1080\" style=\"font-size: var(--articleFontsize);letter-spacing: 0.034em;caret-color: rgba(0, 0, 0, 0);\" data-recalc-dims=\"1\" \/><\/span><\/p>\n<p><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100012897\" data-ratio=\"0.35444444444444445\" data-s=\"300,640\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_j5NCk981eW52iaoQWHeYPNocjwIP0g.jpg?w=640\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAyDVUmkNYAdUJkXGezu5DHEmuDBZBLmQUtd6UXhE7INz4ichtj5NCk981eW52iaoQWHeYPNocjwIP0g\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"900\" style=\"letter-spacing: 0.578px;text-align: center;text-wrap: wrap;\" data-recalc-dims=\"1\" \/><\/p>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u60f3\u8981\u4e00\u952e\u641e\u5b9a\u6240\u6709\u5355\u7ec6\u80de\u6570\u636e\u7684\u6279\u6b21\u6548\u5e94\uff1f\u9664\u975e\u4f60\u7528Harmony \u539f\u521b  \u5c0f\u679c &n […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/60391"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=60391"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/60391\/revisions"}],"predecessor-version":[{"id":60402,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/60391\/revisions\/60402"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=60391"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=60391"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=60391"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}