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auto;background-color: rgb(193, 209, 255);padding: 10px 20px;box-shadow: rgb(255, 255, 255) -2px 2px 0px;\">\n<section style=\"text-align: left;justify-content: flex-start;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;align-self: center;flex: 0 0 auto;width: 100%;height: auto;\">\n<section style=\"text-align: center;color: rgb(0, 0, 0);\" powered-by=\"xiumi.us\">\n<p><strong>\u516c\u4f17\u53f7\u540e\u53f0\u56de\u590d\u201c111\u201d<\/strong><\/p>\n<p><strong>\u9886\u53d6\u672c\u7bc7\u4ee3\u7801\u3001\u57fa\u56e0\u96c6\u6216\u793a\u4f8b\u6570\u636e\u7b49\u6587\u4ef6<\/strong><\/p>\n<p><strong>\u6587\u4ef6\u7f16\u53f7\uff1a240402<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><span style=\"color: rgb(171, 25, 66);\"><strong>\u9700\u8981\u79df\u8d41\u670d\u52a1\u5668\u7684\u5c0f\u4f19\u4f34\u53ef\u4ee5\u626b\u7801\u6dfb\u52a0\u5c0f\u679c\uff0c\u6b64\u5916\u5c0f\u679c\u8fd8\u63d0\u4f9b\u751f\u4fe1\u5206\u6790\uff0c\u601d\u8def\u8bbe\u8ba1\uff0c\u6587\u732e\u590d\u73b0\u7b49\uff0c\u6709\u9700\u8981\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u6765\u64a9~<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100017744\" data-ratio=\"1\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-02_\u6570\u636e\u63a2\u7d22\u4e4bRMA\u9884\u5904\u7406\u3001limma\u5dee\u5f02\u5206\u6790\uff0c\u63ed\u79d8\u57fa\u56e0\u8868\u8fbe\u5965\u79d8\uff01\u70ed\u56fe\u4e0e\u706b\u5c71\u56fe\u5e26\u4f60\u76f4\u89c2\u611f\u53d7\u5dee\u5f02\u8868\u8fbe\u9b45\u529b\uff01\/2_f6PG40Qrdu5e9B1iaIMItPiajABYWA.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxqaqPbQprPd4vw8Ytp0cprbzXiakMDdFriaB6B59ibhlBCrNyQFf6PG40Qrdu5e9B1iaIMItPiajABYWA\/640?wx_fmt=jpeg'\" data-type=\"jpeg\" data-w=\"430\" style=\"width: 170px;height: 170px;\" \/><\/p>\n<\/section>\n<h2 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><strong><span style=\"font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;font-family: Cambria;font-variant: normal;text-transform: none;\">(\u4e00)<\/span><span style=\"font-size: 16px;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u57fa\u672c\u6982\u5ff5\u4ecb\u7ecd<\/span><br \/>\n  <\/span><\/strong><span style=\"font-size: 16px;letter-spacing: 1px;\"> <\/span><\/h2>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;\">\u5c0f\u679c\u5148\u548c\u5c0f\u4f19\u4f34\u4eec\u603b\u8ff0\u4e00\u4e0b\u57fa\u672c\u601d\u8def\uff0c\u9996\u5148\u5229\u7528RMA\u6cd5\u9884\u5904\u7406\u6cd5\u5bf9\u4e24\u7ec4\u6837\u672c\u6570\u636e\u8fdb\u884c\u5904\u7406\uff0c\u7136\u540e\u5bf9\u4e24\u7ec4\u6570\u636e\u8fdb\u884c\u5408\u5e76\uff0c\u5229\u7528limma R\u5305\u5bf9\u5176\u8fdb\u884c\u5dee\u5f02\u5206\u6790\uff0c\u6700\u540e\u518d\u5bf9\u5206\u6790\u7684\u7ed3\u679c\u8fdb\u884c\u53ef\u89c6\u5316\uff01<\/span><\/p>\n<h3 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;color: rgb(0, 213, 255);\">1.RMA\u7b97\u6cd5<\/span><span style=\"font-size: 16px;letter-spacing: 1px;\">  <\/span><\/h3>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;\">RMA\u7b97\u6cd5\u57fa\u4e8e20\u7ec4\u63a2\u9488\u7684\u4fe1\u53f7\u5206\u5e03\u91c7\u7528\u968f\u673a\u6a21\u578b\u6765\u8bc4\u4f30\u8868\u8fbe\u503c\uff0c\u5bf9\u4e8e\u4f4e\u566a\u53f7\u7684\u5b9e\u9a8c\u9002\u7528\u6027\u66f4\u597d\u4e00\u70b9\u3002RMA\u7b97\u6cd5\u4e0d\u76f4\u63a5\u5229\u7528MM\uff08Mismatch\uff0c\u4e0d\u5339\u914d\uff09\u7684\u4fe1\u53f7\u53bb\u9664\u80cc\u666f\u566a\u97f3\uff0c\u800c\u662f\u901a\u8fc7\u7279\u5b9a\u7684\u8ba1\u7b97\u65b9\u5f0f\u6765\u8bc4\u4f30\u548c\u5904\u7406\u6570\u636e\u3002<\/span><\/p>\n<h3 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;color: rgb(0, 213, 255);\">2.Limma R\u5305<\/span><span style=\"font-size: 16px;letter-spacing: 1px;\">  <\/span><\/h3>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;\">limma\u5305\u662fR\u8bed\u8a00\u4e2d\u7684\u4e00\u4e2a\u5e7f\u6cdb\u4f7f\u7528\u4e8e\u5dee\u5f02\u8868\u8fbe\u5206\u6790\u7684\u5305\uff0c\u7279\u522b\u662f\u5904\u7406\u6765\u81ea\u5fae\u9635\u5217\u548c\u9ad8\u901a\u91cf\u6d4b\u5e8f\u7684\u6570\u636e\u3002limma\u5305\u63d0\u4f9b\u4e86\u4e30\u5bcc\u7684\u7edf\u8ba1\u65b9\u6cd5\uff0c\u4ee5\u8bc6\u522b\u5728\u4e24\u7ec4\u6216\u591a\u7ec4\u4e4b\u95f4\u8868\u8fbe\u6c34\u5e73\u663e\u8457\u4e0d\u540c\u7684\u57fa\u56e0\u6216\u8f6c\u5f55\u672c\u3002<\/span><span style=\"font-size: 16px;line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);font-weight: bold;\">\u672c\u6b21\u7684R\u5305\u64cd\u4f5c\u5360\u7528\u5185\u5b58\u6bd4\u8f83\u5927\uff0c\u5efa\u8bae\u4f7f\u7528\u670d\u52a1\u5668\uff0c\u6b22\u8fce\u8054\u7cfb\u5c0f\u679c\u79df\u8d41\u6027\u4ef7\u6bd4\u5c45\u9ad8\u7684\u670d\u52a1\u5668\u54e6\uff01<\/span>    <\/span><o:page><\/o:page><\/p>\n<h3 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;color: rgb(0, 213, 255);\">3.\u5dee\u5f02\u5206\u6790\u7ed3\u679c\u53ef\u89c6\u5316<\/span><span style=\"font-size: 16px;letter-spacing: 1px;\"><br \/>\n  <\/span><\/h3>\n<p style=\"text-align: justify;font-size: 10.5pt;font-family: Calibri;font-weight: normal;margin-left: 46pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;\">1)<\/span><span style=\"font-size: 16px;line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u70ed\u56fe<\/span><\/span><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;\">\u4e3b\u8981\u901a\u8fc7\u989c\u8272\u7684\u6df1\u6d45\u6765\u53cd\u6620\u57fa\u56e0\u5728\u4e0d\u540c\u6837\u672c\u6216\u6761\u4ef6\u4e0b\u7684\u8868\u8fbe\u6c34\u5e73\u3002\u6bcf\u4e2a\u57fa\u56e0\u6216\u6837\u672c\u5728\u70ed\u56fe\u4e2d\u5360\u636e\u4e00\u4e2a\u5355\u5143\u683c\uff0c\u989c\u8272\u6df1\u6d45\u4ee3\u8868\u8868\u8fbe\u91cf\u7684\u5927\u5c0f\u3002\u901a\u8fc7\u70ed\u56fe\uff0c\u6211\u4eec\u53ef\u4ee5\u76f4\u89c2\u5730\u89c2\u5bdf\u57fa\u56e0\u5728\u4e0d\u540c\u6837\u672c\u95f4\u7684\u8868\u8fbe\u5dee\u5f02\uff0c\u4ee5\u53ca\u6837\u672c\u95f4\u7684\u76f8\u4f3c\u6027\u3002<\/span><\/p>\n<p style=\"text-align: justify;font-size: 10.5pt;font-family: Calibri;font-weight: normal;margin-left: 46pt;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;line-height: 150%;font-family: Calibri;font-variant: normal;text-transform: none;\">2)<\/span><span style=\"font-size: 16px;line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u706b\u5c71\u56fe<\/span><\/span><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;\">\u4fa7\u91cd\u4e8e\u5c55\u793a\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u7684\u663e\u8457\u6027\u3002\u5728\u706b\u5c71\u56fe\u4e2d\uff0c\u6bcf\u4e2a\u70b9\u4ee3\u8868\u4e00\u4e2a\u57fa\u56e0\uff0c\u6a2a\u5750\u6807\u8868\u793a\u57fa\u56e0\u5728\u4e0d\u540c\u6837\u672c\u6216\u6761\u4ef6\u4e0b\u7684\u8868\u8fbe\u5dee\u5f02\u500d\u6570\uff08logFC\uff09\uff0c\u7eb5\u5750\u6807\u8868\u793a\u5dee\u5f02\u8868\u8fbe\u7684\u663e\u8457\u6027\u6c34\u5e73\uff08-log10 p\u503c\uff09\u3002\u901a\u8fc7\u706b\u5c71\u56fe\uff0c\u53ef\u4ee5\u5feb\u901f\u5730\u7b5b\u9009\u51fa\u8868\u8fbe\u5dee\u5f02\u663e\u8457\u7684\u57fa\u56e0\uff0c\u5e76\u89c2\u5bdf\u5b83\u4eec\u5728\u6574\u4f53\u57fa\u56e0\u8868\u8fbe\u8c31\u4e2d\u7684\u5206\u5e03\u60c5\u51b5\u3002<\/span><\/p>\n<h2 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><strong><span style=\"font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;font-family: Cambria;font-variant: normal;text-transform: none;\">(\u4e8c)<\/span><span style=\"font-size: 16px;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u4ee3\u7801\u5b9e\u64cd<\/span><br \/>\n  <\/span><\/strong><span style=\"font-size: 16px;letter-spacing: 1px;\"> <\/span><\/h2>\n<p style=\"margin-left: 21pt;text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;\">\u5e9f\u8bdd\u4e0d\u591a\u8bf4\uff0c\u5c0f\u679c\u76f4\u63a5\u5e26\u5c0f\u4f19\u4f34\u4eec\u5b9e\u64cd\uff01<\/span><span style=\"color: rgb(0, 176, 240);font-family: Calibri;font-size: 16px;letter-spacing: 1px;text-indent: 0em;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"powershell\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#RMA\u6cd5\u9884\u5904\u7406normal\u6837\u672c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">\"C:\\Users\\sfksfj\\Desktop\\GSE123924_RAW\\N\"<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\">library(affyPLM)<\/span><\/code><code><span class=\"code-snippet_outer\">library(affy)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">Data<\/span><-ReadAffy()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#**************sampleNames(Data)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#***************N=length(Data)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7528RMA\u9884\u5904\u7406\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">eset.rma<-rma(<span class=\"code-snippet__keyword\">Data<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u83b7\u53d6\u8868\u8fbe\u6570\u636e\u5e76\u8f93\u51fa\u5230\u8868\u683c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">normal_exprs<-exprs(eset.rma) <\/span><\/code><code><span class=\"code-snippet_outer\">probeid<-rownames(normal_exprs)<\/span><\/code><code><span class=\"code-snippet_outer\">normal_exprs<-cbind(probeid,normal_exprs) <\/span><\/code><code><span class=\"code-snippet_outer\">write.table(normal_exprs,file=<span class=\"code-snippet__string\">\"normal.expres.txt\"<\/span>,sep=<span class=\"code-snippet__string\">'t'<\/span>,quote=F,row.names=F)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);font-size: 16px;letter-spacing: 1px;\"><\/span><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;\"> <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100017745\" data-ratio=\"0.7152496626180836\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-02_\u6570\u636e\u63a2\u7d22\u4e4bRMA\u9884\u5904\u7406\u3001limma\u5dee\u5f02\u5206\u6790\uff0c\u63ed\u79d8\u57fa\u56e0\u8868\u8fbe\u5965\u79d8\uff01\u70ed\u56fe\u4e0e\u706b\u5c71\u56fe\u5e26\u4f60\u76f4\u89c2\u611f\u53d7\u5dee\u5f02\u8868\u8fbe\u9b45\u529b\uff01\/3_2a36jflGAiciaXk7KH8gQFHN6BPZrw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxsjeKQ4XGjX2sicUyfCbiadOzAYUglf4WkKz9A7hUXPYc3poNb2a36jflGAiciaXk7KH8gQFHN6BPZrw\/640?wx_fmt=png&from=appmsg'\" data-type=\"png\" data-w=\"741\" style=\"\" \/><span style=\"font-family: Calibri;font-size: 16px;letter-spacing: 1px;text-align: justify;text-indent: 0em;\"> <\/span><span style=\"font-family: Calibri;font-size: 16px;letter-spacing: 1px;text-align: justify;text-indent: 0em;\">   <\/span><span style=\"font-family: Calibri;font-size: 16px;letter-spacing: 1px;text-align: justify;text-indent: 0em;\">   <\/span><o:p style=\"font-family: Calibri;font-size: 16px;letter-spacing: 1px;text-align: justify;text-indent: 0em;\"> <\/o:p><span style=\"color: rgb(0, 176, 240);font-family: Calibri;font-size: 16px;letter-spacing: 1px;text-align: justify;text-indent: 0em;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"powershell\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#RMA\u6cd5\u9884\u5904\u7406tumor\u6837\u672c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">\"C:\\Users\\sfksfj\\Desktop\\GSE123924_RAW\\T\"<\/span>) <span class=\"code-snippet__comment\">#\u81ea\u5df1\u8bbe\u5b9a<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">library(affyPLM)<\/span><\/code><code><span class=\"code-snippet_outer\">library(affy)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__keyword\">Data<\/span><-ReadAffy()<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#**************sampleNames(Data)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#**************N=length(Data)<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u7528RMA\u9884\u5904\u7406\u6570\u636e<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">eset.rma<-rma(<span class=\"code-snippet__keyword\">Data<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u83b7\u53d6\u8868\u8fbe\u6570\u636e\u5e76\u8f93\u51fa\u5230\u8868\u683c<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">normal_exprs<-exprs(eset.rma)<\/span><\/code><code><span class=\"code-snippet_outer\">probeid<-rownames(normal_exprs)<\/span><\/code><code><span class=\"code-snippet_outer\">normal_exprs<-cbind(probeid,normal_exprs) <\/span><\/code><code><span class=\"code-snippet_outer\">write.table(normal_exprs,file=<span class=\"code-snippet__string\">\"tumor.expres.txt\"<\/span>,sep=<span class=\"code-snippet__string\">'t'<\/span>,quote=F,row.names=F)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;letter-spacing: 1px;\"> <\/span><span style=\"font-family: Calibri;font-size: 16px;letter-spacing: 1px;text-indent: 0em;\">     <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100017746\" data-ratio=\"0.7547169811320755\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-02_\u6570\u636e\u63a2\u7d22\u4e4bRMA\u9884\u5904\u7406\u3001limma\u5dee\u5f02\u5206\u6790\uff0c\u63ed\u79d8\u57fa\u56e0\u8868\u8fbe\u5965\u79d8\uff01\u70ed\u56fe\u4e0e\u706b\u5c71\u56fe\u5e26\u4f60\u76f4\u89c2\u611f\u53d7\u5dee\u5f02\u8868\u8fbe\u9b45\u529b\uff01\/4_glpstUVaRF7DWgbpT2B1YSmOsfoiaw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxsjeKQ4XGjX2sicUyfCbiadOj9ZzPP51hyseUq2PBV3lRur2RglpstUVaRF7DWgbpT2B1YSmOsfoiaw\/640?wx_fmt=png&from=appmsg'\" data-type=\"png\" data-w=\"742\" style=\"\" \/><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;\">   <o:p> <\/o:p><\/span><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 240);font-size: 16px;letter-spacing: 1px;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"typescript\"><code><span class=\"code-snippet_outer\">#\u5408\u5e76N\u548cT\u7684\u6570\u636e<\/span><\/code><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">\"C:\\Users\\sfksfj\\Desktop\\\u5dee\u5f02\u5206\u6790\u6e90\u6587\u4ef6\\GSE123924_RAW\\C\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">normal_exprs<-read.table(<span class=\"code-snippet__string\">\"normal.expres.txt\"<\/span>,header=T,sep=<span class=\"code-snippet__string\">\"t\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">tumor_exprs<-read.table(<span class=\"code-snippet__string\">\"tumor.expres.txt\"<\/span>,header=T,sep=<span class=\"code-snippet__string\">\"t\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">#T\u548cN\u5408\u5e76<\/span><\/code><code><span class=\"code-snippet_outer\">probe_exprs<-merge(normal_exprs,tumor_exprs,by=<span class=\"code-snippet__string\">\"probeid\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">write.table(probe_exprs,file=<span class=\"code-snippet__string\">\"cancer.probeid.exprs.txt\"<\/span>,sep=<span class=\"code-snippet__string\">'t'<\/span>,quote=F,row.names=F)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\">#Probe ID\u8f6c\u6362\u4e3aGene symbol\uff08\u5148\u5bf9\u5e73\u53f0\u6587\u4ef6\u8fdb\u884c\u6574\u7406\uff09*************************<\/span><\/code><code><span class=\"code-snippet_outer\">setwd(<span class=\"code-snippet__string\">\"C:\\Users\\sfksfj\\Desktop\\\u5dee\u5f02\u5206\u6790\u6e90\u6587\u4ef6\\GSE123924_RAW\\C\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u8bfb\u53d6\u57fa\u56e0\u8868\u8fbe\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">probe_exp<-read.table(<span class=\"code-snippet__string\">\"cancer.probeid.exprs.txt\"<\/span>,header=T,sep=<span class=\"code-snippet__string\">\"t\"<\/span>,row.names=<span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u8bfb\u53d6\u5e73\u53f0\u63a2\u9488\u6587\u4ef6<\/span><\/code><code><span class=\"code-snippet_outer\">probeid_geneid<-read.table(<span class=\"code-snippet__string\">\"platform.txt\"<\/span>,header=T,sep=<span class=\"code-snippet__string\">\"t\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">probe_name<-rownames(probe_exp)<\/span><\/code><code><span class=\"code-snippet_outer\">#probe\u8fdb\u884c\u5339\u914d<\/span><\/code><code><span class=\"code-snippet_outer\">loc<-match(probeid_geneid[,<span class=\"code-snippet__number\">1<\/span>],probe_name)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u786e\u5b9a\u80fd\u5339\u914d\u4e0a\u7684probe\u8868\u8fbe\u503c<\/span><\/code><code><span class=\"code-snippet_outer\">probe_exp<-probe_exp[loc,]<\/span><\/code><code><span class=\"code-snippet_outer\">#\u6bcf\u4e2aprobeid\u5e94\u5bf9\u7684geneid<\/span><\/code><code><span class=\"code-snippet_outer\">raw_geneid<-<span class=\"code-snippet__keyword\">as<\/span>.numeric(<span class=\"code-snippet__keyword\">as<\/span>.matrix(probeid_geneid[,<span class=\"code-snippet__number\">3<\/span>]))<\/span><\/code><code><span class=\"code-snippet_outer\">#\u627e\u51fa\u6709geneid\u7684probeid\u5e76\u5efa\u7acb\u7d22\u5f15<\/span><\/code><code><span class=\"code-snippet_outer\">index<-which(!is.na(raw_geneid))<\/span><\/code><code><span class=\"code-snippet_outer\">#\u63d0\u53d6\u6709geneid\u7684probe <\/span><\/code><code><span class=\"code-snippet_outer\">geneid<-raw_geneid[index]<\/span><\/code><code><span class=\"code-snippet_outer\">#\u627e\u5230\u6bcf\u4e2ageneid\u7684\u8868\u8fbe\u503c<\/span><\/code><code><span class=\"code-snippet_outer\">exp_matrix<-probe_exp[index,]<\/span><\/code><code><span class=\"code-snippet_outer\">geneidfactor<-factor(geneid)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u591a\u4e2a\u63a2\u9488\u5bf9\u5e94<span class=\"code-snippet__number\">1<\/span>\u4e2a\u57fa\u56e0\u7684\u60c5\u51b5\uff0c\u53d6\u5e73\u5747\u503c<\/span><\/code><code><span class=\"code-snippet_outer\">gene_exp_matrix<-apply(exp_matrix,<span class=\"code-snippet__number\">2<\/span>,<span class=\"code-snippet__function\"><span class=\"code-snippet__keyword\">function<\/span>(<span class=\"code-snippet__params\">x<\/span>) <span class=\"code-snippet__title\">tapply<\/span>(<span class=\"code-snippet__params\">x,geneidfactor,mean<\/span>))<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">#<span class=\"code-snippet__title\">geneid<\/span>\u4f5c\u4e3a\u884c\u540d<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">rownames<\/span>(<span class=\"code-snippet__params\">gene_exp_matrix<\/span>)<-<span class=\"code-snippet__title\">levels<\/span>(<span class=\"code-snippet__params\">geneidfactor<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">geneid<\/span><-<span class=\"code-snippet__title\">rownames<\/span>(<span class=\"code-snippet__params\">gene_exp_matrix<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">gene_exp_matrix2<\/span><-<span class=\"code-snippet__title\">cbind<\/span>(<span class=\"code-snippet__params\">geneid,gene_exp_matrix<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">write<\/span>.<span class=\"code-snippet__title\">table<\/span>(<span class=\"code-snippet__params\">gene_exp_matrix2,file=\"Gastric.cancer.geneid.exprs.txt\",sep='t',quote=F,row.names=F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5c06<span class=\"code-snippet__title\">gene<\/span> <span class=\"code-snippet__title\">id<\/span> \u8f6c\u6362\u4e3a<span class=\"code-snippet__title\">gene<\/span> <span class=\"code-snippet__title\">symbol<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">loc<\/span><-<span class=\"code-snippet__title\">match<\/span>(<span class=\"code-snippet__params\">rownames(gene_exp_matrix),probeid_geneid[,3]<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">rownames<\/span>(<span class=\"code-snippet__params\">gene_exp_matrix<\/span>)=<span class=\"code-snippet__title\">probeid_geneid<\/span>[<span class=\"code-snippet__title\">loc<\/span>,2]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">genesymbol<\/span><-<span class=\"code-snippet__title\">rownames<\/span>(<span class=\"code-snippet__params\">gene_exp_matrix<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">gene_exp_matrix3<\/span><-<span class=\"code-snippet__title\">cbind<\/span>(<span class=\"code-snippet__params\">genesymbol,gene_exp_matrix<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">write<\/span>.<span class=\"code-snippet__title\">table<\/span>(<span class=\"code-snippet__params\">gene_exp_matrix3,file=\"Gastric.cancer.genesyb.exprs.txt\",sep='t',quote=F,row.names=F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\">#\u8865\u5145\u7f3a\u5931\u503c(<span class=\"code-snippet__params\">\u9700\u8981\u5bf9Gastric.cancer.genesyb.exprs.txt\u8fdb\u884c\u5904\u7406\uff0c\u67e5\u627e\u5e74\u53f7\uff0c\u5220\u9664\u65e0\u540d\u57fa\u56e0<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u6700\u8fd1\u90bb\u5c45\u6cd5\uff08<span class=\"code-snippet__title\">KNN<\/span>\uff0c<span class=\"code-snippet__title\">k<\/span>-<span class=\"code-snippet__title\">Nearest<\/span> <span class=\"code-snippet__title\">Neighbor<\/span>\uff09\u6cd5\uff1a\u6b64\u65b9\u6cd5\u662f\u5bfb\u627e\u548c\u6709\u7f3a\u5931\u503c\u7684\u57fa\u56e0\u7684\u8868\u8fbe\u8c31\u76f8\u4f3c\u7684\u5176\u4ed6\u57fa\u56e0<\/span><\/code><code><span class=\"code-snippet_outer\">#\u901a\u8fc7\u8fd9\u4e9b\u57fa\u56e0\u7684\u8868\u8fbe\u503c\uff08\u4f9d\u7167\u8868\u8fbe\u8c31\u76f8\u4f3c\u6027\u52a0\u6743\uff09\u6765\u586b\u5145\u7f3a\u5931\u503c<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">setwd<\/span>(<span class=\"code-snippet__params\">\"C:\\Users\\sfksfj\\Desktop\\\u5dee\u5f02\u5206\u6790\u6e90\u6587\u4ef6\\GSE123924_RAW\\C\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u8c03\u7528\u51fd\u6570<span class=\"code-snippet__title\">impute<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">library<\/span>(<span class=\"code-snippet__params\">impute<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u8bfb\u53d6\u8868\u8fbe\u503c<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">gene_exp_matrix<\/span><-<span class=\"code-snippet__title\">read<\/span>.<span class=\"code-snippet__title\">table<\/span>(<span class=\"code-snippet__params\">\"Gastric.cancer.genesyb.exprs.txt\",header=T,sep=\"t\",row.names=1<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">gene_exp_matrix<\/span><-<span class=\"code-snippet__title\">as<\/span>.<span class=\"code-snippet__title\">matrix<\/span>(<span class=\"code-snippet__params\">gene_exp_matrix<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">#<span class=\"code-snippet__title\">KNN<\/span>\u6cd5\u8ba1\u7b97\u7f3a\u5931\u503c<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">imputed_gene_exp<\/span><-<span class=\"code-snippet__title\">impute<\/span>.<span class=\"code-snippet__title\">knn<\/span>(<span class=\"code-snippet__params\">gene_exp_matrix,k=10,rowmax=0.5,colmax=0.8,maxp=3000,rng.seed=362436069<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u8bfb\u51fa\u7ecf\u8fc7\u7f3a\u5931\u503c\u5904\u7406\u7684\u6570\u636e<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">GeneExp<\/span><-<span class=\"code-snippet__title\">imputed_gene_exp$data<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">#\u5199\u5165\u8868\u683c<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">genesymbol<\/span><-<span class=\"code-snippet__title\">rownames<\/span>(<span class=\"code-snippet__params\">GeneExp<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">GeneExp<\/span><-<span class=\"code-snippet__title\">cbind<\/span>(<span class=\"code-snippet__params\">genesymbol,GeneExp<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">write<\/span>.<span class=\"code-snippet__title\">table<\/span>(<span class=\"code-snippet__params\">GeneExp,file=\"Gastric.cancer.gene.exprs.txt\",sep='t',quote=F,row.names=F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\">#***********************\uff08\u6839\u636e\u6837\u672c\u586b\u5199\u6570\u91cf\uff09<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">setwd<\/span>(<span class=\"code-snippet__params\">\"C:\\Users\\sfksfj\\Desktop\\\u5dee\u5f02\u5206\u6790\u6e90\u6587\u4ef6\\GSE123924_RAW\\C\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">library<\/span>(<span class=\"code-snippet__params\">limma<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">rt<\/span><-<span class=\"code-snippet__title\">read<\/span>.<span class=\"code-snippet__title\">table<\/span>(<span class=\"code-snippet__params\">\"Gastric.cancer.gene.exprs.txt\",header=T,sep=\"t\",row.names=\"genesymbol\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">class<\/span><-<span class=\"code-snippet__title\">c<\/span>(<span class=\"code-snippet__params\">rep(<span class=\"code-snippet__string\">\"normal\"<\/span>,<span class=\"code-snippet__number\">3<\/span>),rep(<span class=\"code-snippet__string\">\"tumor\"<\/span>,<span class=\"code-snippet__number\">3<\/span>)<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u6837\u672c\u6570\u91cf\u5373\u4e3a\u751f\u6210\u77e9\u9635\u7684<span class=\"code-snippet__title\">CEL<\/span>\u6587\u4ef6\u6570\u91cf<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">design<\/span><-<span class=\"code-snippet__title\">model<\/span>.<span class=\"code-snippet__title\">matrix<\/span>(<span class=\"code-snippet__params\">~factor(<span class=\"code-snippet__keyword\">class<\/span>)<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">colnames<\/span>(<span class=\"code-snippet__params\">design<\/span>)<-<span class=\"code-snippet__title\">c<\/span>(<span class=\"code-snippet__params\">\"normal\",\"tumor\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">fit<\/span><-<span class=\"code-snippet__title\">lmFit<\/span>(<span class=\"code-snippet__params\">rt,design<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">fit2<\/span><-<span class=\"code-snippet__title\">eBayes<\/span>(<span class=\"code-snippet__params\">fit<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">allDiff<\/span>=<span class=\"code-snippet__title\">topTable<\/span>(<span class=\"code-snippet__params\">fit2,adjust='fdr',coef=2,<span class=\"code-snippet__built_in\">number<\/span>=200000<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">write<\/span>.<span class=\"code-snippet__title\">table<\/span>(<span class=\"code-snippet__params\">allDiff,file=\"limmaTab.xls\",sep=\"t\",quote=F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">diffLab<\/span><-<span class=\"code-snippet__title\">allDiff<\/span>[<span class=\"code-snippet__title\">with<\/span>(<span class=\"code-snippet__params\">allDiff, ((logFC><span class=\"code-snippet__number\">1<\/span> |logFC<(-<span class=\"code-snippet__number\">1<\/span>)) & adj.P.Val<<span class=\"code-snippet__number\">0.05<\/span>)<\/span>),]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">write<\/span>.<span class=\"code-snippet__title\">table<\/span>(<span class=\"code-snippet__params\">diffLab,file=\"diffEXp.xls\",sep=\"t\",quote=F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">#\u5171\u8868\u8fbe<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">diffExpLevel<\/span><-<span class=\"code-snippet__title\">rt<\/span>[<span class=\"code-snippet__title\">rownames<\/span>(<span class=\"code-snippet__params\">diffLab<\/span>),]<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">qvalue<\/span>=<span class=\"code-snippet__title\">allDiff<\/span>[<span class=\"code-snippet__title\">rownames<\/span>(<span class=\"code-snippet__params\">diffLab<\/span>),]<span class=\"code-snippet__title\">$adj<\/span>.<span class=\"code-snippet__title\">P<\/span>.<span class=\"code-snippet__title\">Val<\/span><\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">diffExpQvalue<\/span>=<span class=\"code-snippet__title\">cbind<\/span>(<span class=\"code-snippet__params\">qvalue,diffExpLevel<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">write<\/span>.<span class=\"code-snippet__title\">table<\/span>(<span class=\"code-snippet__params\">diffExpQvalue,file=\"diffExpLevel.xls\",sep=\"t\",quote=F<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"> <\/span><\/code><code><span class=\"code-snippet_outer\">#\u70ed\u56fe<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">hmExp<\/span>=<span class=\"code-snippet__title\">log10<\/span>(<span class=\"code-snippet__params\">diffExpLevel+0.00001<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">library<\/span>(<span class=\"code-snippet__params\">'gplots'<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">hmMat<\/span>=<span class=\"code-snippet__title\">as<\/span>.<span class=\"code-snippet__title\">matrix<\/span>(<span class=\"code-snippet__params\">hmExp<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">pdf<\/span>(<span class=\"code-snippet__params\">file=\"heatmap.pdf\",height=120,width=90<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">par<\/span>(<span class=\"code-snippet__params\">oma=c(<span class=\"code-snippet__number\">3<\/span>,<span class=\"code-snippet__number\">3<\/span>,<span class=\"code-snippet__number\">3<\/span>,<span class=\"code-snippet__number\">5<\/span>)<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">heatmap<\/span>.2(<span class=\"code-snippet__params\">hmMat,col='greenred',trace=\"none\",cexCol=1<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__title\">dev<\/span>.<span class=\"code-snippet__title\">off<\/span>(<span class=\"code-snippet__params\"><\/span>)<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);font-size: 16px;letter-spacing: 1px;\"><\/span><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);font-size: 16px;letter-spacing: 1px;\">      <\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100017747\" data-ratio=\"1.340495867768595\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-02_\u6570\u636e\u63a2\u7d22\u4e4bRMA\u9884\u5904\u7406\u3001limma\u5dee\u5f02\u5206\u6790\uff0c\u63ed\u79d8\u57fa\u56e0\u8868\u8fbe\u5965\u79d8\uff01\u70ed\u56fe\u4e0e\u706b\u5c71\u56fe\u5e26\u4f60\u76f4\u89c2\u611f\u53d7\u5dee\u5f02\u8868\u8fbe\u9b45\u529b\uff01\/5_ElvA0ozpZnQf4029bQ6CQfSIm7WF3w.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxsjeKQ4XGjX2sicUyfCbiadOibOibS68Oh53yROef4aVbKlegibElvA0ozpZnQf4029bQ6CQfSIm7WF3w\/640?wx_fmt=png&from=appmsg'\" data-type=\"png\" data-w=\"605\" style=\"\" \/><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);font-size: 16px;letter-spacing: 1px;\">    <o:p> <\/o:p><\/span><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 240);font-size: 16px;letter-spacing: 1px;\"><\/span><\/p>\n<section class=\"code-snippet__fix code-snippet__js\">\n<ul class=\"code-snippet__line-index code-snippet__js\">\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<li><\/li>\n<\/ul>\n<pre class=\"code-snippet__js\" data-lang=\"perl\"><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u706b\u5c71\u56fe<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">pdf(file=<span class=\"code-snippet__string\">\"vol.pdf\"<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">xMax=max(-log1<span class=\"code-snippet__number\">0<\/span>(allDiff$adj.P.Val))<\/span><\/code><code><span class=\"code-snippet_outer\">yMax=max(<span class=\"code-snippet__keyword\">abs<\/span>(allDiff$logFC))<\/span><\/code><code><span class=\"code-snippet_outer\">plot(-log1<span class=\"code-snippet__number\">0<\/span>(allDiff$adj.P.Val),allDiff$logFC,xlab=<span class=\"code-snippet__string\">\"adj.P.Val\"<\/span>,ylab=<span class=\"code-snippet__string\">\"logFC\"<\/span>,main=<span class=\"code-snippet__string\">\"Volcano\"<\/span>,xlim=c(<span class=\"code-snippet__number\">0<\/span>,xMax),ylim=c(-yMax,yMax),pch=<span class=\"code-snippet__number\">20<\/span>,cex=<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">4<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">diffSub=subset(allDiff,allDiff$adj.P.Val<<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">05<\/span> & <span class=\"code-snippet__keyword\">abs<\/span>(allDiff$logFC)><span class=\"code-snippet__number\">1<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\"><span class=\"code-snippet__comment\">#\u53ef\u4fee\u6539\uff0c\u6700\u4f4e\u4e3a0\uff0clogFC\u503c\u8d8a\u5927\u5dee\u5f02\u500d\u6570\u8d8a\u9ad8<\/span><\/span><\/code><code><span class=\"code-snippet_outer\">points(-log1<span class=\"code-snippet__number\">0<\/span>(diffSub$adj.P.Val),diffSub$logFC,pch=<span class=\"code-snippet__number\">20<\/span>,col=<span class=\"code-snippet__string\">\"red\"<\/span>,cex=<span class=\"code-snippet__number\">0<\/span>.<span class=\"code-snippet__number\">4<\/span>)<\/span><\/code><code><span class=\"code-snippet_outer\">abline(h=<span class=\"code-snippet__number\">0<\/span>,lty=<span class=\"code-snippet__number\">2<\/span>,lwd=<span class=\"code-snippet__number\">3<\/span>) <\/span><\/code><code><span class=\"code-snippet_outer\">dev.off()<\/span><\/code><\/pre>\n<\/section>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;color: rgb(0, 176, 80);font-size: 16px;letter-spacing: 1px;\"><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100017748\" data-ratio=\"1.0030721966205838\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-02_\u6570\u636e\u63a2\u7d22\u4e4bRMA\u9884\u5904\u7406\u3001limma\u5dee\u5f02\u5206\u6790\uff0c\u63ed\u79d8\u57fa\u56e0\u8868\u8fbe\u5965\u79d8\uff01\u70ed\u56fe\u4e0e\u706b\u5c71\u56fe\u5e26\u4f60\u76f4\u89c2\u611f\u53d7\u5dee\u5f02\u8868\u8fbe\u9b45\u529b\uff01\/6_6ysndYVBwTjBiabiaQMQWibXSjIRtA.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxsjeKQ4XGjX2sicUyfCbiadOdX2E4EFdyZKc9NCxuvGjG3FC8Ho6ysndYVBwTjBiabiaQMQWibXSjIRtA\/640?wx_fmt=png&from=appmsg'\" data-type=\"png\" data-w=\"651\" style=\"\" \/><\/p>\n<h2 style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><strong><span style=\"font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;font-family: Cambria;font-variant: normal;text-transform: none;\">(\u4e09)<\/span><span style=\"font-size: 16px;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u6587\u7ae0\u5c0f\u7ed3<\/span><\/span><\/strong><span style=\"font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\"><\/span><br \/>\n  <\/span><\/h2>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;font-size: 16px;letter-spacing: 1px;\">\u4eca\u5929\uff0c\u5c0f\u679c\u4e3a\u5927\u5bb6\u8be6\u5c3d\u5730\u5c55\u793a\u4e86\u9ad8\u901a\u91cf\u57fa\u56e0\u8868\u8fbe\u6570\u636e\uff08\u5373microarray\u6570\u636e\uff09\u4ece\u9884\u5904\u7406\u5230\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u518d\u5230\u7ed3\u679c\u53ef\u89c6\u5316\u7684\u6574\u4e2a\u6d41\u7a0b\u3002<\/span><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u9996\u5148\uff0c\u6211\u4eec\u5bf9normal\u6837\u672c\u548ctumor\u6837\u672c\u6570\u636e\u8fdb\u884c\u4e86\u9884\u5904\u7406\uff0c\u786e\u4fdd\u4e86\u6570\u636e\u7684\u51c6\u786e\u6027\u548c\u53ef\u9760\u6027\u3002\u63a5\u7740\uff0c\u6211\u4eec\u5c06normal\u548ctumor\u6837\u672c\u6570\u636e\u8fdb\u884c\u4e86\u5408\u5e76\uff0c\u4ee5\u4fbf\u5728\u7edf\u4e00\u7684\u6846\u67b6\u4e0b\u8fdb\u884c\u6bd4\u8f83\u548c\u5206\u6790\uff0c\u8fdb\u800c\u63ed\u793a\u4e0d\u540c\u6837\u672c\u95f4\u7684\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\u3002\u4e3a\u4e86\u65b9\u4fbf\u7406\u89e3\u548c\u89e3\u91ca\uff0c\u6211\u4eec\u5c06Probe ID\u8f6c\u6362\u4e3a\u4e86Gene symbol\uff0c\u4f7f\u5f97\u6bcf\u4e2a\u57fa\u56e0\u7684\u8868\u8fbe\u53d8\u5316\u90fd\u80fd\u591f\u76f4\u89c2\u5730\u5bf9\u5e94\u5230\u5177\u4f53\u7684\u57fa\u56e0\u540d\u79f0\u4e0a\u3002\u6b64\u5916\uff0c\u9488\u5bf9\u6570\u636e\u4e2d\u53ef\u80fd\u51fa\u73b0\u7684\u7f3a\u5931\u503c\uff0c\u6211\u4eec\u4e5f\u8fdb\u884c\u4e86\u76f8\u5e94\u7684\u5904\u7406\uff0c\u786e\u4fdd\u5206\u6790\u7684\u5b8c\u6574\u6027\u548c\u51c6\u786e\u6027\u3002\u7d27\u63a5\u7740\uff0c\u6211\u4eec\u8fdb\u884c\u4e86\u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff0c\u901a\u8fc7\u4e00\u7cfb\u5217\u7edf\u8ba1\u548c\u8ba1\u7b97\u65b9\u6cd5\uff0c\u8bc6\u522b\u51fa\u5728normal\u548ctumor\u6837\u672c\u95f4\u8868\u8fbe\u6c34\u5e73\u663e\u8457\u4e0d\u540c\u7684\u57fa\u56e0\u3002\u6700\u540e\uff0c\u4e3a\u4e86\u66f4\u76f4\u89c2\u5730\u5c55\u793a\u8fd9\u4e9b\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u8868\u8fbe\u6a21\u5f0f\u548c\u663e\u8457\u6027\uff0c\u6211\u4eec\u751f\u6210\u4e86\u70ed\u56fe\u548c\u706b\u5c71\u56fe\u3002\u70ed\u56fe\u4ee5\u989c\u8272\u7684\u6df1\u6d45\u5c55\u793a\u4e86\u57fa\u56e0\u5728\u4e0d\u540c\u6837\u672c\u95f4\u7684\u8868\u8fbe\u5dee\u5f02\uff0c\u800c\u706b\u5c71\u56fe\u5219\u6e05\u6670\u5730\u6807\u51fa\u4e86\u8868\u8fbe\u5dee\u5f02\u663e\u8457\u7684\u57fa\u56e0\uff0c\u4f7f\u6211\u4eec\u8fc5\u901f\u6355\u6349\u5230\u5173\u952e\u4fe1\u606f\u3002<\/span>    <\/span><o:page><\/o:page><\/p>\n<p style=\"text-align: justify;text-indent: 0em;margin-top: 8px;margin-bottom: 0px;line-height: 1.6em;\"><span style=\"font-size: 16px;letter-spacing: 1px;\"><span style=\"font-size: 16px;line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u6574\u4e2a\u6d41\u7a0b\u4e0d\u4ec5\u6709\u52a9\u4e8e\u6211\u4eec\u6df1\u5165\u7406\u89e3\u57fa\u56e0\u8868\u8fbe\u7684\u53d8\u5316\u89c4\u5f8b\uff0c\u8fd8\u4e3a\u540e\u7eed\u7684\u76f8\u5173\u751f\u7269\u5b66\u5b9e\u9a8c\u548c\u75be\u75c5\u7814\u7a76\u63d0\u4f9b\u4e86\u91cd\u8981\u7684\u53c2\u8003\u4f9d\u636e\u3002<\/span><span style=\"font-size: 16px;line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);font-weight: bold;\">\u5982\u679c\u5404\u4f4d\u89c9\u5f97\u81ea\u5df1\u8fd0\u884c\u4ee3\u7801\u592a\u9ebb\u70e6\uff0c\u6b22\u8fce\u7528\u6211\u4eec\u7684\u4e91\u751f\u4fe1\u5c0f\u5de5\u5177\uff0c\u53ea\u8981\u8f93\u5165\u5408\u9002\u7684\u6570\u636e\u5c31\u53ef\u4ee5\u76f4\u63a5\u51fa\u60f3\u8981\u7684\u56fe\u5462\uff0c\u9644\u4e91\u751f\u4fe1\u94fe\u63a5\uff08<\/span><\/span><span style=\"font-family: Calibri;font-variant: normal;text-transform: none;font-weight: bold;font-size: 16px;letter-spacing: 1px;\">http:\/\/www.biocloudservice.com\/home.html<\/span><span style=\"font-size: 16px;letter-spacing: 1px;\"><span style=\"letter-spacing: 1px;font-size: 16px;line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;color: rgb(255, 0, 0);font-weight: bold;\">\uff09\u3002<\/span><span style=\"letter-spacing: 1px;font-size: 16px;line-height: 150%;font-family: \u5b8b\u4f53;font-variant: normal;text-transform: none;\">\u5e0c\u671b\u672c\u671f\u5185\u5bb9\u80fd\u591f\u5bf9\u5c0f\u4f19\u4f34\u4eec\u6709\u6240\u542f\u53d1\u548c\u5e2e\u52a9\uff0c\u8ba9\u6211\u4eec\u4e00\u8d77\u5728\u751f\u7269\u4fe1\u606f\u5b66\u7684\u6d77\u6d0b\u4e2d\u63a2\u7d22\u66f4\u591a\u672a\u77e5\u7684\u5965\u79d8\u5427\uff01<\/span>    <\/span><o:page><\/o:page><\/p>\n<p style=\"text-indent: 0px;margin-bottom: 0px;\"><span style=\"color: rgb(0, 122, 170);\"><strong><span style=\"color: rgb(0, 122, 170);letter-spacing: 0.578px;text-indent: 32px;text-wrap: wrap;background-color: rgb(255, 255, 255);font-size: 15px;\">\u5c0f\u679c\u8fd8<span style=\"color: rgb(0, 122, 170);background-color: rgb(255, 255, 255);font-size: 15px;letter-spacing: 0.578px;text-decoration: none solid rgba(0, 0, 0, 0.9);\">\u63d0\u4f9b\u601d\u8def\u8bbe\u8ba1\u3001\u5b9a\u5236\u751f\u4fe1\u5206\u6790\u3001\u6587\u732e\u601d\u8def\u590d\u73b0\uff1b\u6709\u9700\u8981\u7684\u5c0f\u4f19\u4f34\u6b22\u8fce\u76f4\u63a5\u626b\u7801\u54a8\u8be2\u5c0f\u679c\uff0c\u7aed\u8bda\u4e3a\u60a8\u7684\u79d1\u7814\u52a9\u529b\uff01<\/span><\/span><\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-galleryid=\"\" data-imgfileid=\"100017513\" data-ratio=\"0.7621359223300971\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-02_\u6570\u636e\u63a2\u7d22\u4e4bRMA\u9884\u5904\u7406\u3001limma\u5dee\u5f02\u5206\u6790\uff0c\u63ed\u79d8\u57fa\u56e0\u8868\u8fbe\u5965\u79d8\uff01\u70ed\u56fe\u4e0e\u706b\u5c71\u56fe\u5e26\u4f60\u76f4\u89c2\u611f\u53d7\u5dee\u5f02\u8868\u8fbe\u9b45\u529b\uff01\/7_XiafCUEp1NqZoBA3F9mvRcJR4PQ1Tw.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAyZkFgOs95yxYHqY7n8a8k4wWKhXgwQvZVTwDhgYYDDu7asGXiafCUEp1NqZoBA3F9mvRcJR4PQ1Tw\/640?wx_fmt=png&from=appmsg'\" data-type=\"png\" data-w=\"618\" style=\"\" \/><\/p>\n<section style=\"font-size: 15px;padding-right: 2px;padding-left: 2px;margin-bottom: 0px;\">\n<section style=\"text-align: center;justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;flex: 0 0 auto;height: auto;border-color: rgb(114, 114, 114);border-width: 1px;border-style: solid;box-shadow: rgb(194, 209, 255) -5px 5px 0px;align-self: flex-start;\">\n<section style=\"justify-content: center;display: flex;flex-flow: row;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;width: 42.6829%;vertical-align: middle;box-shadow: rgb(43, 65, 110) 0px 0px 0px;flex: 0 0 auto;height: auto;padding-left: 10px;align-self: center;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;line-height: 0;transform: translate3d(20px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;width: 75%;height: auto;\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100017749\" data-ratio=\"1\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-02_\u6570\u636e\u63a2\u7d22\u4e4bRMA\u9884\u5904\u7406\u3001limma\u5dee\u5f02\u5206\u6790\uff0c\u63ed\u79d8\u57fa\u56e0\u8868\u8fbe\u5965\u79d8\uff01\u70ed\u56fe\u4e0e\u706b\u5c71\u56fe\u5e26\u4f60\u76f4\u89c2\u611f\u53d7\u5dee\u5f02\u8868\u8fbe\u9b45\u529b\uff01\/8_B2jkicacLzPjw4xMSqVvoucZjonVDA.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAxVYfSsM0wdf9d3RpNiaTcHohxPNEVs2VFhPMukpU8lchxrvcB2jkicacLzPjw4xMSqVvoucZjonVDA\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"430\" style=\"vertical-align: middle;width: 100%;\" \/><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: 56.9106%;flex: 0 0 auto;height: auto;align-self: center;\">\n<section style=\"line-height: 2;letter-spacing: 1px;color: rgb(43, 65, 110);\" powered-by=\"xiumi.us\">\n<p style=\"margin-bottom: 2px;\"><strong>\u5b9a\u5236\u751f\u4fe1\u5206\u6790<\/strong><\/p>\n<p style=\"margin-bottom: 2px;\"><strong>\u670d\u52a1\u5668\u79df\u8d41<\/strong><\/p>\n<p><strong>\u626b\u7801\u54a8\u8be2\u5c0f\u679c<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p powered-by=\"xiumi.us\"><br \/><\/p>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497780&idx=2&sn=7eee90af6f387e4153bba13028e545a1&chksm=cf7f68f1f808e1e7dfb3a0597ef442e5c6e700ecf6ee5a035c766a4c7486c12eabd0ab218ea6&scene=21#wechat_redirect\" textvalue=\"\u4f60\u5df2\u9009\u4e2d\u4e86\u6dfb\u52a0\u94fe\u63a5\u7684\u5185\u5bb9\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"1\" rel=\"noopener\"><span class=\"js_jump_icon h5_image_link\"><img decoding=\"async\" class=\"rich_pages wxw-img\" data-imgfileid=\"100017751\" data-ratio=\"0.3253333333333333\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-02_\u6570\u636e\u63a2\u7d22\u4e4bRMA\u9884\u5904\u7406\u3001limma\u5dee\u5f02\u5206\u6790\uff0c\u63ed\u79d8\u57fa\u56e0\u8868\u8fbe\u5965\u79d8\uff01\u70ed\u56fe\u4e0e\u706b\u5c71\u56fe\u5e26\u4f60\u76f4\u89c2\u611f\u53d7\u5dee\u5f02\u8868\u8fbe\u9b45\u529b\uff01\/9_7TvAyUibd1TPhJmZfialJtv6tu2SEg.png\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_png\/zcicibWZessAxVYfSsM0wdf9d3RpNiaTcHoj9xlsoyiaBvzaezDUFAicibMAF8ts7TvAyUibd1TPhJmZfialJtv6tu2SEg\/640?wx_fmt=png&from=appmsg'\" data-type=\"png\" data-w=\"750\" style=\"vertical-align: middle;width: 100%;\" \/><\/span><\/a><\/section>\n<\/section>\n<section style=\"text-align: center;margin-top: 10px;margin-bottom: 10px;line-height: 0;\" powered-by=\"xiumi.us\">\n<section style=\"vertical-align: middle;display: inline-block;line-height: 0;\"><img decoding=\"async\" class=\"rich_pages wxw-img js_insertlocalimg\" data-imgfileid=\"100017750\" data-ratio=\"0.3811111111111111\" data-s=\"300,640\" src=\"\u56fe\u7247\/\u751f\u4fe1\u679c_2024-04-02_\u6570\u636e\u63a2\u7d22\u4e4bRMA\u9884\u5904\u7406\u3001limma\u5dee\u5f02\u5206\u6790\uff0c\u63ed\u79d8\u57fa\u56e0\u8868\u8fbe\u5965\u79d8\uff01\u70ed\u56fe\u4e0e\u706b\u5c71\u56fe\u5e26\u4f60\u76f4\u89c2\u611f\u53d7\u5dee\u5f02\u8868\u8fbe\u9b45\u529b\uff01\/10_9N02aBaau07RufXeXRLmvAHsrFZxtw.jpg\" onerror=\"this.src='https:\/\/mmbiz.qpic.cn\/sz_mmbiz_jpg\/zcicibWZessAz5wg6Ne6sYkannSE9NiatkcPh3icbKanJagacjU6LqJrGAL29N02aBaau07RufXeXRLmvAHsrFZxtw\/640?wx_fmt=jpeg&from=appmsg'\" data-type=\"jpeg\" data-w=\"900\" style=\"letter-spacing: 0.578px;text-align: center;text-wrap: wrap;\" \/><\/section>\n<\/section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;text-align: left;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;min-width: 10%;flex: 0 0 auto;height: auto;align-self: flex-end;padding-right: 2px;padding-left: 2px;\">\n<section style=\"margin: 10px 0% 2px;transform: translate3d(2px, 0px, 0px);\" powered-by=\"xiumi.us\">\n<section style=\"font-size: 16px;color: rgb(58, 66, 94);line-height: 1;letter-spacing: 5px;text-align: center;\">\n<p><strong>\u5f80\u671f\u56de\u987e<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"height: 3px;background-color: rgb(95, 156, 239);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: 2px;margin-right: 0%;margin-left: 0%;text-align: center;line-height: 0.8;font-size: 9px;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;border-top: 0.8em solid rgb(58, 66, 94);border-left: 0.7em solid transparent !important;border-right: 0.7em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: bottom;width: auto;flex: 100 100 0%;align-self: flex-end;height: auto;\">\n<section style=\"margin-right: 0%;margin-left: 0%;\" powered-by=\"xiumi.us\">\n<section style=\"height: 3px;background-color: rgb(95, 156, 239);\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<section style=\"margin-top: 2px;margin-right: 0%;margin-left: 0%;text-align: center;line-height: 0.8;font-size: 9px;\" powered-by=\"xiumi.us\">\n<section style=\"width: 0px;display: inline-block;border-top: 0.8em solid rgba(255, 255, 255, 0);border-left: 0.7em solid transparent !important;border-right: 0.7em solid transparent !important;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"min-height: 40px;margin: 10px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"width: 100%;margin-right: auto;margin-bottom: -10px;margin-left: auto;\">\n<table width=\"100%\">\n<tbody>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:0\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:0.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;box-shadow: rgb(0, 0, 0) 0px 0px 0px;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>01<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247497780&idx=2&sn=7eee90af6f387e4153bba13028e545a1&chksm=cf7f68f1f808e1e7dfb3a0597ef442e5c6e700ecf6ee5a035c766a4c7486c12eabd0ab218ea6&scene=21#wechat_redirect\" textvalue=\"1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff0c\u4e24\u4eba\u6210\u56e2\uff0c1\u4eba\u514d\u5355\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">1024G\u5b58\u50a8\u7684\u751f\u4fe1\u670d\u52a1\u5668\uff0c\u4e24\u4eba\u6210\u56e2\uff0c1\u4eba\u514d\u5355\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:1\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:1.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>02<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498878&idx=1&sn=fc34ed3f1d15d060bbaf8166adcebd58&chksm=cf7f6cbbf808e5ad0b5417e50ed75cfbd1b3a83ac43b97f1dfa718c822878a9a004990075133&scene=21#wechat_redirect\" textvalue=\"\u5355\u4e2a\u6570\u636e\u5e93\u7528\u817b\u4e86\uff1f\u591a\u6570\u636e\u5e93\u201c\u7ec4\u5408\u62f3\u201d\u5e26\u4f60\u6253\u5f00\u514d\u75ab\u6d78\u6da6\u65b0\u601d\u8def\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u5355\u4e2a\u6570\u636e\u5e93\u7528\u817b\u4e86\uff1f\u591a\u6570\u636e\u5e93\u201c\u7ec4\u5408\u62f3\u201d\u5e26\u4f60\u6253\u5f00\u514d\u75ab\u6d78\u6da6\u65b0\u601d\u8def\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:2\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:2.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>03<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498673&idx=1&sn=1e09b74a836c1e64a62ae1f66eeb3f93&chksm=cf7f6b74f808e262de70be43fd73bf5fefdc0914e525eb2032f741a0152e619689cedc76946f&scene=21#wechat_redirect\" textvalue=\"\u5b5f\u5fb7\u5c14\u968f\u673a\u5316\u7684\u51c6\u5907\u5de5\u4f5c\uff0cGWAS\u6570\u636e\u7684\u7f51\u7ad9\u4e0b\u8f7d\u65b9\u6cd5\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"11\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u5b5f\u5fb7\u5c14\u968f\u673a\u5316\u7684\u51c6\u5907\u5de5\u4f5c\uff0cGWAS\u6570\u636e\u7684\u7f51\u7ad9\u4e0b\u8f7d\u65b9\u6cd5<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<tr opera-tn-ra-comp=\"_$.pages:0.layers:0.comps:4.classicTable1:3\" powered-by=\"xiumi.us\">\n<td colspan=\"1\" rowspan=\"1\" opera-tn-ra-cell=\"_$.pages:0.layers:0.comps:4.classicTable1:3.td@@0\" style=\"border-color: rgb(62, 62, 62);border-style: none;padding: 0px;\" width=\"100.0000%\">\n<section>\n<section style=\"display: flex;flex-flow: row;margin-top: 10px;margin-right: 0%;margin-left: 0%;justify-content: flex-start;\" powered-by=\"xiumi.us\">\n<section style=\"display: inline-block;vertical-align: middle;width: auto;min-width: 10%;height: auto;flex: 0 0 auto;align-self: center;\">\n<section style=\"font-size: 19px;color: rgb(95, 156, 239);line-height: 1;\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><strong>04<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"display: inline-block;vertical-align: middle;width: auto;flex: 100 100 0%;align-self: center;height: auto;\">\n<section style=\"color: rgb(140, 140, 140);\" powered-by=\"xiumi.us\">\n<p style=\"text-wrap: wrap;\"><span style=\"color: rgb(224, 224, 224);\">\uff5c<a target=\"_blank\" href=\"http:\/\/mp.weixin.qq.com\/s?__biz=Mzg3OTg3MjQyNg==&mid=2247498517&idx=1&sn=fa3c0104cee316982fe8162b79fe234f&chksm=cf7f6bd0f808e2c6cf979704bf883cfb5480444604f90da61dc286e7541fa5bb49df0a2fb58a&scene=21#wechat_redirect\" textvalue=\"\u8ddf\u7740\u5c0f\u679c\u5b66\u590d\u73b0-\u624b\u628a\u624b\u5e26\u4f60\u62ff\u4e0bIF=46.9Nature \u7ea7\u522b\u7684\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u56fe\uff01\uff01\" linktype=\"text\" imgurl=\"\" imgdata=\"null\" data-itemshowtype=\"0\" tab=\"innerlink\" data-linktype=\"2\" rel=\"noopener\">\u8ddf\u7740\u5c0f\u679c\u5b66\u590d\u73b0-\u624b\u628a\u624b\u5e26\u4f60\u62ff\u4e0bIF=46.9Nature \u7ea7\u522b\u7684\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\u56fe\uff01\uff01<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin: 5px 0%;\" powered-by=\"xiumi.us\">\n<section style=\"background-color: rgb(224, 224, 224);height: 1px;\"><svg viewbox=\"0 0 1 1\" style=\"float:left;line-height:0;width:0;vertical-align:top;\"><\/svg><\/section>\n<\/section>\n<\/section>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: center;margin-bottom: 0px;\"><br \/><\/p>\n<p style=\"display: none;\"><mp-style-type data-value=\"3\"><\/mp-style-type><\/p>\n<p><\/content>\n<\/div>\n<p><\/body><br \/>\n<\/html><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6570\u636e\u63a2\u7d22\u4e4bRMA\u9884\u5904\u7406\u3001limma\u5dee\u5f02\u5206\u6790\uff0c\u63ed\u79d8\u57fa\u56e0\u8868\u8fbe\u5965\u79d8\uff01\u70ed\u56fe\u4e0e\u706b\u5c71\u56fe\u5e26\u4f60\u76f4\u89c2\u611f\u53d7\u5dee\u5f02\u8868\u8fbe\u9b45\u529b\uff01 \u5c0f\u679c&n […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/60562"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=60562"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/60562\/revisions"}],"predecessor-version":[{"id":60564,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/60562\/revisions\/60564"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=60562"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=60562"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=60562"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}