{"id":61140,"date":"2024-10-17T11:07:57","date_gmt":"2024-10-17T03:07:57","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=61140"},"modified":"2024-10-17T11:07:57","modified_gmt":"2024-10-17T03:07:57","slug":"%e8%ae%a9%e6%88%91%e7%9c%8b%e7%9c%8b%e6%98%af%e8%b0%81%e8%bf%98%e5%9c%a8%e7%94%a8%e7%bd%91%e9%a1%b5%e8%b7%91blast%ef%bc%8cblast%e4%b8%89%e5%88%86%e9%92%9f%e8%ae%a9%e4%bd%a0%e7%9a%84%e5%ba%8f","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=61140","title":{"rendered":"\u8ba9\u6211\u770b\u770b\u662f\u8c01\u8fd8\u5728\u7528\u7f51\u9875\u8dd1BLAST\uff0cBLAST+,\u4e09\u5206\u949f\u8ba9\u4f60\u7684\u5e8f\u5217\u6bd4\u5bf9\u6548\u7387\u66f4\u4e0a\u4e00\u5c42\u697c\uff01\uff01\uff01"},"content":{"rendered":"\n

<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u540c\u5b66\u4eec\uff0c\u4eca\u5929\u5927\u6d77\u54e5\u8981\u5411\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e2a\u5728\u5206\u5b50\u751f\u7269\u5b66\u9886\u57df\u4e2d\u975e\u5e38\u91cd\u8981\u7684\u8f6f\u4ef6\u2014\u2014BLAST+\uff01BLAST+ \u662f\u4e00\u6b3e\u7528\u4e8e\u5e8f\u5217\u6bd4\u5bf9\u7684\u5f3a\u5927\u5de5\u5177\u3002\u8981\u77e5\u9053\uff0c\u5728\u751f\u7269\u5e8f\u5217\u4e2d\uff0c\u6bd4\u8f83\u4e0d\u540c\u5e8f\u5217\u4e4b\u95f4\u7684\u76f8\u4f3c\u6027\u53ef\u4ee5\u5e2e\u52a9\u6211\u4eec\u63ed\u793a\u5b83\u4eec\u7684\u8fdb\u5316\u5173\u7cfb\u3001\u529f\u80fd\u7279\u6027\u548c\u6f5c\u5728\u7684\u751f\u7269\u5b66\u610f\u4e49\u3002\u901a\u8fc7 BLAST+\uff0c\u6211\u4eec\u53ef\u4ee5\u5feb\u901f\u3001\u9ad8\u6548\u5730\u6bd4\u5bf9 DNA\u3001RNA \u548c\u86cb\u767d\u8d28\u5e8f\u5217\uff0c\u53d1\u73b0\u540c\u6e90\u57fa\u56e0\uff0c\u8fdb\u884c\u529f\u80fd\u6ce8\u91ca\uff0c\u4ee5\u53ca\u63a2\u7d22\u8fdb\u5316\u8fc7\u7a0b\u4e2d\u7684\u5173\u952e\u53d8\u5316\u3002\u5e8f\u5217\u6bd4\u5bf9\u5bf9\u5927\u6d77\u54e5\u6765\u8bf4\u6839\u672c\u4e0d\u662f\u96be\u9898<\/strong>\uff0c\u8981\u662f<\/strong>\u540c\u5b66\u4eec<\/strong>\u6709\u81ea\u5df1\u505a\u4e0d\u4e86\u7684\u751f\u4fe1\u5206\u6790\uff0c<\/strong>\u6b22\u8fce\u968f\u65f6<\/strong>\u8054\u7cfb\u6211<\/strong>\uff01\uff01\uff01<\/strong><\/strong><\/p>\n\n\n\n

\u5728\u672c\u6b21\u5b66\u4e60\u4e2d\uff0c\u6211\u4eec\u4e0d\u4ec5\u5c06\u5b66\u4f1a\u5982\u4f55\u5b89\u88c5\u548c\u4f7f\u7528 BLAST+\uff0c\u8fd8\u5c06\u638c\u63e1\u5bf9\u5e8f\u5217\u6570\u636e\u8fdb\u884c\u6bd4\u5bf9\u548c\u5206\u6790\u7684\u65b9\u6cd5\u3002\u901a\u8fc7\u5b9e\u9645\u793a\u4f8b\u7684\u6f14\u793a\uff0c\u6211\u4eec\u5c06\u66f4\u6df1\u5165\u5730\u7406\u89e3\u5e8f\u5217\u76f8\u4f3c\u6027\u7684\u8ba1\u7b97\u539f\u7406\u548c\u6bd4\u5bf9\u7ed3\u679c\u7684\u89e3\u8bfb\u6280\u5de7\u3002BLAST+ \u4e0d\u4ec5\u53ef\u4ee5\u5e2e\u52a9\u6211\u4eec\u8bc6\u522b\u5df2\u77e5\u7684\u57fa\u56e0\u548c\u529f\u80fd\u5143\u4ef6\uff0c\u8fd8\u53ef\u4ee5\u53d1\u73b0\u6f5c\u5728\u7684\u65b0\u57fa\u56e0\u548c\u53d8\u5f02\uff0c\u4e3a\u57fa\u56e0\u529f\u80fd\u7814\u7a76\u548c\u751f\u7269\u5b66\u63a2\u7d22\u63d0\u4f9b\u4e86\u91cd\u8981\u652f\u6301\u3002\u901a\u8fc7\u4eca\u5929\u7684\u5b66\u4e60\uff0c\u540c\u5b66\u4eec\u5c06\u80fd\u591f\u66f4\u52a0\u719f\u7ec3\u5730\u5e94\u7528 BLAST+\uff0c\u4ece\u800c\u5728\u57fa\u56e0\u7ec4\u5b66\u3001\u529f\u80fd\u57fa\u56e0\u7ec4\u5b66\u548c\u8fdb\u5316\u751f\u7269\u5b66\u7684\u7814\u7a76\u4e2d\u53d6\u5f97\u66f4\u5927\u7684\u8fdb\u5c55\u3002\u63a5\u4e0b\u6765\uff0c\u540c\u5b66\u4eec\u8ddf\u968f\u5927\u6d77\u54e5\u7684\u6b65\u4f10\uff0c\u8ba9\u6211\u4eec\u6b63\u5f0f\u5f00\u542f\u5bf9 BLAST+ \u7684\u5b66\u4e60\u4e4b\u65c5\u5427\uff01<\/p>\n\n\n\n

\u672c\u6b21\u4ecb\u7ecd\u7684\u5de5\u5177\u9700\u8981\u5728<\/strong>\u670d\u52a1\u5668<\/strong>\u4e0a\u624d\u80fd\u6b63\u5e38<\/strong>\u8fd0\u884c\uff0c<\/strong>\u540c\u5b66\u4eec<\/strong>\u5982\u679c\u6ca1\u6709\u81ea\u5df1\u7684\u670d\u52a1\u5668\u6b22\u8fce\u8054\u7cfb\u6211\u4eec<\/strong>\u8fdb\u884c<\/strong>\u670d\u52a1\u5668\u79df\u8d41~<\/strong><\/strong><\/p>\n\n\n\n

BLAST+\u8f6f\u4ef6\u4ecb\u7ecd<\/p>\n\n\n\n

BLAST+ \u662f\u4e00\u5957\u7528\u4e8e\u5e8f\u5217\u6bd4\u5bf9\u7684\u8f6f\u4ef6\u5957\u4ef6\uff0c\u5176\u4e2d\u5305\u62ec\u4e86\u591a\u79cd\u7248\u672c\u7684 BLAST \u7a0b\u5e8f\uff0c\u5982 blastp \u7528\u4e8e\u86cb\u767d\u8d28\u5e8f\u5217\u6bd4\u5bf9\u3002\u901a\u8fc7\u8ba1\u7b97\u5f97\u5206\u548c\u671f\u671b\u503c\uff08E-value\uff09\uff0cblastp \u80fd\u6709\u6548\u5730\u6bd4\u5bf9\u86cb\u767d\u8d28\u5e8f\u5217\uff0c\u627e\u51fa\u76f8\u4f3c\u6027\u548c\u540c\u6e90\u6027\u3002\u7528\u6237\u53ef\u4ee5\u901a\u8fc7\u8c03\u6574\u53c2\u6570\u4f18\u5316\u6bd4\u5bf9\u7ed3\u679c\uff0c\u5982\u8bbe\u7f6e\u9608\u503c\u3001\u9009\u62e9\u5408\u9002\u7684\u5e8f\u5217\u6570\u636e\u5e93\u3002BLAST+ \u63d0\u4f9b\u4e86\u9ad8\u6548\u7684\u5e76\u884c\u8ba1\u7b97\u652f\u6301\uff0c\u9002\u7528\u4e8e\u5e7f\u6cdb\u7684\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\uff0c\u4ece\u57fa\u7840\u7684\u5e8f\u5217\u76f8\u4f3c\u6027\u5206\u6790\u5230\u590d\u6742\u7684\u529f\u80fd\u6ce8\u91ca\u548c\u8fdb\u5316\u7814\u7a76\u3002<\/p>\n\n\n\n

BLAST+\u8f6f\u4ef6\u5b89\u88c5<\/p>\n\n\n\n

BLAST+\u5fc5\u987b\u8981\u5728\u670d\u52a1\u5668\u4e0a\u8fd0\u884c\uff0c\u9700\u8981\u540c\u5b66\u4eec\u6709\u4e00\u4e9bLinux\u7cfb\u7edf\u7684\u57fa\u7840\u77e5\u8bc6\uff0c\u5982\u679c\u6ca1\u6709\u4e5f\u4e0d\u7528\u62c5\u5fc3\uff0c\u5927\u6d77\u54e5\u5e26\u4f60\u4e00\u6b65\u4e00\u6b65\u6765\uff0c\u8ddf\u7d27\u5927\u6d77\u54e5\u7684\u6b65\u4f10\uff0c\u8ba9\u6211\u4eec\u4eceBLAST+\u7684\u5b89\u88c5\u5f00\u59cb\uff0c\u6162\u6162\u5b66\u4e60\u5982\u4f55\u4f7f\u7528\u8fd9\u6b3e\u5f3a\u5927\u7684\u5de5\u5177\u5427\u3002<\/p>\n\n\n\n

\u9700\u8981\u7684\u7cfb\u7edf\uff1aLinux\u7cfb\u7edf\uff0c\u9700\u8981\u7684\u8f6f\u4ef6\u652f\u6301\uff1aconda<\/p>\n\n\n\n

\u4e3a\u4e86\u907f\u514d\u6211\u4eec\u73b0\u5728\u7684\u7cfb\u7edf\u73af\u5883\u4e0d\u7b26\u5408BLAST+\u8f6f\u4ef6\u7684\u8981\u6c42\uff0c\u6240\u4ee5\u6211\u4eec\u9700\u8981\u4e3aBLAST+\u5b89\u88c5\u4e00\u4e2a\u865a\u62df\u7684\u5de5\u4f5c\u73af\u5883\uff0c\u5728\u4e0d\u66f4\u6539\u73b0\u5728\u7cfb\u7edf\u73af\u5883\u7684\u524d\u63d0\u4e0b\uff0c\u5b89\u88c5BLAST+\uff0c\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

conda create –name blast+ # \u521b\u5efa\u4e00\u4e2aBLAST+\u73af\u5883<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u9047\u5230\u56fe\u4e0a\u63d0\u793a\uff0c\u8f93\u5165y\u5373\u53ef<\/p>\n\n\n\n

\u521b\u5efa\u5b8c\u73af\u5883\u540e\uff0c\u6211\u4eec\u6fc0\u6d3b\u73af\u5883\uff0c\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

conda activate blast + # \u6fc0\u6d3bBLAST+\u73af\u5883<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u663e\u793a\uff08blast+\uff09\u5219\u8868\u660e\u6211\u4eec\u5df2\u7ecf\u6210\u529f\u521b\u5efa\u5e76\u4e14\u6fc0\u6d3b\u4e86BLAST+\u73af\u5883\uff0c\u63a5\u7740\u6211\u4eec\u5c31\u53ef\u4ee5\u5728\u8be5\u73af\u5883\u4e0b\u5b89\u88c5BLAST+\u4e86\uff0c\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

conda install blast # \u5728conda\u73af\u5883\u4e2d\u5b89\u88c5blast<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u9047\u5230\u56fe\u4e0a\u63d0\u793a\uff0c\u8f93\u5165y\u5373\u53ef<\/p>\n\n\n\n

\u8010\u5fc3\u7b49\u5f85\u5b89\u88c5\u5b8c\u6210\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u8f93\u5165\u4ee5\u4e0b\u547d\u4ee4\u6d4b\u8bd5\u662f\u5426\u5b89\u88c5\u6210\u529f\u3002<\/p>\n\n\n\n

blastp -help #\u5524\u9192BLAST+ \u53c2\u8003\u624b\u518c<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u5982\u679c\u663e\u793a\u5982\u56fe\u6240\u793a\u7684\u547d\u4ee4\u53c2\u6570\u63d0\u793a\uff0c\u5c31\u8868\u660e\u5df2\u7ecf\u6210\u529f\u5b89\u88c5\u4e86BLAST+\u7a0b\u5e8f\u3002<\/p>\n\n\n\n

\u4f7f\u7528BLAST+\u8fdb\u884c\u86cb\u767d\u8d28\u5e8f\u5217\u6bd4\u5bf9<\/p>\n\n\n\n

BLAST+\u4e3b\u8981\u547d\u4ee4 <\/strong><\/strong><\/p>\n\n\n\n

BLAST+ \u662f NCBI \u63d0\u4f9b\u7684\u4e00\u7ec4\u7528\u4e8e\u5e8f\u5217\u6bd4\u5bf9\u548c\u5206\u6790\u7684\u5de5\u5177\u96c6\uff0c\u5305\u62ec\u591a\u79cd\u547d\u4ee4\u548c\u7a0b\u5e8f\uff0c\u6bcf\u4e2a\u547d\u4ee4\u7528\u4e8e\u4e0d\u540c\u7c7b\u578b\u7684\u5e8f\u5217\u6bd4\u5bf9\u3002\u4ee5\u4e0b\u662f BLAST+ \u4e2d\u4e00\u4e9b\u4e3b\u8981\u7684\u547d\u4ee4\u53ca\u5176\u7b80\u8981\u4ecb\u7ecd\uff1a<\/p>\n\n\n\n

blastp\uff1a\u7528\u4e8e\u6bd4\u5bf9\u86cb\u767d\u8d28\u5e8f\u5217\u4e0e\u86cb\u767d\u8d28\u6570\u636e\u5e93\u3002<\/p>\n\n\n\n

blastn\uff1a\u7528\u4e8e\u6bd4\u5bf9\u6838\u9178\u5e8f\u5217\u4e0e\u6838\u9178\u6570\u636e\u5e93\u3002<\/p>\n\n\n\n

blastx\uff1a\u7528\u4e8e\u5c06\u672a\u77e5\u7684\u6838\u9178\u5e8f\u5217\u7ffb\u8bd1\u6210\u6c28\u57fa\u9178\u5e8f\u5217\uff0c\u7136\u540e\u4e0e\u86cb\u767d\u8d28\u6570\u636e\u5e93\u6bd4\u5bf9\u3002<\/p>\n\n\n\n

tblastn\uff1a\u7528\u4e8e\u5c06\u86cb\u767d\u8d28\u5e8f\u5217\u7ffb\u8bd1\u6210\u6838\u9178\u5e8f\u5217\uff0c\u7136\u540e\u4e0e\u6838\u9178\u6570\u636e\u5e93\u6bd4\u5bf9\u3002<\/p>\n\n\n\n

tblastx\uff1a\u7528\u4e8e\u6bd4\u5bf9\u6838\u9178\u5e8f\u5217\u7684\u516d\u79cd\u7ffb\u8bd1\u6846\uff08\u4e09\u79cd\u6b63\u5411\u52a0\u4e09\u79cd\u53cd\u5411\uff09\u4e0e\u6838\u9178\u6570\u636e\u5e93\u7684\u516d\u79cd\u7ffb\u8bd1\u6846\u3002<\/p>\n\n\n\n

makeblastdb\uff1a\u7528\u4e8e\u521b\u5efa BLAST \u6570\u636e\u5e93\u3002<\/p>\n\n\n\n

\u4f7f\u7528blastp\u8fdb\u884c\u86cb\u767d\u8d28\u5e8f\u5217\u6bd4\u5bf9 <\/strong><\/strong><\/p>\n\n\n\n

\u5728\u672c\u6587\u4e2d\uff0c\u5927\u6d77\u54e5\u4e3b\u8981\u5411\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e0bblastp\u547d\u4ee4\u7684\u8bed\u6cd5\uff0c\u6211\u4eec\u5c06\u5bf9\u4eceUniport\u83b7\u53d6\u7684\u4eba\u4f53\u548c\u5927\u9f20\u7684\u8840\u7ea2\u86cb\u767d\u03b2\u4e9a\u57fa\uff08Hemoglobin subunit beta, HBB\uff09\u86cb\u767d\u8d28\u5e8f\u5217\u8fdb\u884c\u6bd4\u5bf9\u3002<\/p>\n\n\n\n

\u9996\u5148\u6211\u4eec\u9700\u8981\u4eceUniport\u4e0a\u4e0b\u8f7d\u4eba\u4f53\u548c\u5927\u9f20\u7684\u8840\u7ea2\u86cb\u767d\u03b2\u4e9a\u57fa\u7684\u6c28\u57fa\u9178\u5e8f\u5217\uff0c\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

wget -c https:\/\/rest.uniprot.org\/uniprotkb\/P68871.fasta<\/a> #\u4f7f\u7528wget\u547d\u4ee4\u4e0b\u8f7d\u4eba\u4f53\u8840\u7ea2\u86cb\u767d\u03b2\u4e9a\u57fa\u7684\u6c28\u57fa\u9178\u5e8f\u5217<\/p>\n\n\n\n

wget -c https:\/\/rest.uniprot.org\/uniprotkb\/P11517.fasta #\u4f7f\u7528wget\u547d\u4ee4\u4e0b\u5927\u9f20\u8840\u7ea2\u86cb\u767d\u03b2\u4e9a\u57fa\u7684\u6c28\u57fa\u9178\u5e8f\u5217<\/p>\n\n\n\n

mv P68871.fasta .\/Human.fasta #\u66f4\u6539\u6587\u4ef6\u540d\u79f0<\/p>\n\n\n\n

mv P11517.fasta .\/Rat.fasta #\u66f4\u6539\u6587\u4ef6\u540d\u79f0<\/p>\n\n\n\n

ls #\u67e5\u770b\u4e0b\u8f7d\u7684\u6587\u4ef6<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u663e\u793a\u5982\u4e0a\u56fe\uff0c\u5219\u8868\u793a\u6211\u4eec\u5df2\u7ecf\u6210\u529f\u4e0b\u8f7d\u4e86\u4eba\u4f53\u548c\u5927\u9f20\u7684\u8840\u7ea2\u86cb\u767d\u03b2\u4e9a\u57fa\u7684\u6c28\u57fa\u9178\u5e8f\u5217<\/p>\n\n\n\n

\u63a5\u7740\uff0c\u6211\u4eec\u4f7f\u7528blastp\u547d\u4ee4\u8fdb\u884c\u86cb\u767d\u8d28\u7cfb\u5217\u6bd4\u5bf9\uff0c\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

makeblastdb -in Rat.fasta -dbtype prot -out Rat_db<\/p>\n\n\n\n

# \u521b\u5efa BLAST \u6570\u636e\u5e93 -in Rat.fasta\uff1a\u6307\u5b9a\u8f93\u5165\u7684 FASTA \u683c\u5f0f\u7684\u6587\u4ef6\uff08\u8fd9\u91cc\u662f\u5927\u9f20\u7684\u5e8f\u5217\uff09-dbtype prot\uff1a\u6307\u5b9a\u6570\u636e\u5e93\u7c7b\u578b\u4e3a\u86cb\u767d\u8d28 -out Rat_db\uff1a\u6307\u5b9a\u8f93\u51fa\u7684\u6570\u636e\u5e93\u540d\u79f0\u4e3a Rat_db<\/p>\n\n\n\n

blastp -query Human.fasta -db Rat_db -out results.txt<\/p>\n\n\n\n

# \u8fd0\u884c BLASTP \u8fdb\u884c\u86cb\u767d\u8d28\u5e8f\u5217\u6bd4\u5bf9 -query Human.fasta\uff1a\u6307\u5b9a\u67e5\u8be2\u5e8f\u5217\u6587\u4ef6\uff08\u8fd9\u91cc\u662f\u4eba\u7c7b\u7684\u5e8f\u5217\uff09-db Rat_db\uff1a\u6307\u5b9a\u8981\u6bd4\u5bf9\u7684\u6570\u636e\u5e93\u540d\u79f0\uff08\u8fd9\u91cc\u662f\u521a\u521b\u5efa\u7684\u5927\u9f20\u6570\u636e\u5e93\uff09-out results.txt\uff1a\u6307\u5b9a\u8f93\u51fa\u6587\u4ef6\u540d\u79f0\u4e3a results.txt<\/p>\n\n\n\n

\u86cb\u767d\u8d28\u5e8f\u5217\u6bd4\u5bf9\u7ed3\u679c\u67e5\u770b\u548c\u89e3\u6790<\/strong><\/strong><\/p>\n\n\n\n

\u4ee5\u4e0a\u547d\u4ee4\u8fd0\u884c\u7ed3\u675f\u540e\uff0c\u4f1a\u5728\u5f53\u524d\u76ee\u5f55\u8f93\u51fa\u4e00\u4e2aresults.txt\u6587\u4ef6\uff0c\u5373\u6bd4\u5bf9\u7684\u7ed3\u679c\uff0c\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528cat\u547d\u4ee4\u67e5\u770b\u3002<\/p>\n\n\n\n

cat results.txt #\u67e5\u770b\u6bd4\u5bf9\u7ed3\u679c<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u663e\u793a\u5982\u4e0a\u56fe\uff0c\u5176\u4e2d<\/p>\n\n\n\n

Score (246 bits \/ 629)\uff1a\u6bd4\u5bf9\u5f97\u5206\u8868\u793a\u67e5\u8be2\u5e8f\u5217\u4e0e\u76ee\u6807\u5e8f\u5217\u7684\u5339\u914d\u8d28\u91cf\u3002246 bits \u662f\u6807\u51c6\u5316\u540e\u7684\u5206\u6570\uff0c629 \u662f\u539f\u59cb\u5f97\u5206\u3002\u9ad8\u5206\u6570\u8868\u793a\u6bd4\u5bf9\u8d28\u91cf\u597d\u3002<\/p>\n\n\n\n

Expect (2e-90)\uff1a\u671f\u671b\u503c\u8868\u793a\u5728\u968f\u673a\u5e8f\u5217\u4e2d\u51fa\u73b0\u8fd9\u79cd\u5339\u914d\u7684\u6982\u7387\u30022e-90 \u8868\u793a\u6982\u7387\u975e\u5e38\u4f4e\uff0c\u8868\u660e\u6bd4\u5bf9\u7ed3\u679c\u975e\u5e38\u663e\u8457\u3002<\/p>\n\n\n\n

Identities (116\/147, 79%)\uff1a\u5728147\u4e2a\u6bd4\u5bf9\u4f4d\u7f6e\u4e2d\uff0c\u6709116\u4e2a\u4f4d\u7f6e\u7684\u6b8b\u57fa\u5b8c\u5168\u5339\u914d\uff0c\u5360\u6bd479%\u3002\u8fd9\u8868\u793a\u67e5\u8be2\u5e8f\u5217\u548c\u76ee\u6807\u5e8f\u5217\u5728\u8fd9\u4e9b\u4f4d\u7f6e\u662f\u5b8c\u5168\u76f8\u540c\u7684\u3002<\/p>\n\n\n\n

Positives (128\/147, 87%)\uff1a\u5728147\u4e2a\u6bd4\u5bf9\u4f4d\u7f6e\u4e2d\uff0c\u6709128\u4e2a\u4f4d\u7f6e\u7684\u6b8b\u57fa\u8981\u4e48\u5b8c\u5168\u5339\u914d\uff0c\u8981\u4e48\u662f\u4fdd\u5b88\u66ff\u6362\uff0c\u5360\u6bd487%\u3002\u4fdd\u5b88\u66ff\u6362\u8868\u793a\u6b8b\u57fa\u867d\u7136\u4e0d\u540c\uff0c\u4f46\u5177\u6709\u76f8\u4f3c\u7684\u5316\u5b66\u6027\u8d28\u3002<\/p>\n\n\n\n

Gaps (0\/147, 0%)\uff1a\u5728147\u4e2a\u6bd4\u5bf9\u4f4d\u7f6e\u4e2d\uff0c\u6ca1\u6709\u63d2\u5165\u6216\u7f3a\u5931\u60c5\u51b5\uff0c\u8868\u793a\u4e24\u4e2a\u5e8f\u5217\u5728\u6bd4\u5bf9\u8fc7\u7a0b\u4e2d\u6ca1\u6709\u95f4\u9699\u3002<\/p>\n\n\n\n

\u603b\u4f53\u800c\u8a00\uff0c\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\u4eba\u4f53\u548c\u5927\u9f20\u7684\u8840\u7ea2\u86cb\u767d\u03b2\u4e9a\u57fa\u7684\u6c28\u57fa\u9178\u5e8f\u5217\u5728\u7ed3\u6784\u548c\u529f\u80fd\u4e0a\u53ef\u80fd\u5177\u6709\u9ad8\u5ea6\u7684\u76f8\u4f3c\u6027\u3002<\/p>\n\n\n\n

\u4ee5\u4e0a\u5c31\u662f\u5bf9\u4e8e BLAST+ \u8f6f\u4ef6\u7684\u5168\u90e8\u4ecb\u7ecd\u4e86\u3002\u901a\u8fc7\u672c\u6587\uff0c\u6211\u4eec\u4e86\u89e3\u4e86 BLAST+ \u4f5c\u4e3a\u5f3a\u5927\u7684\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\u7684\u529f\u80fd\u548c\u5e94\u7528\u573a\u666f\u3002BLAST+ \u7ed3\u5408\u4e86\u7075\u6d3b\u6027\u548c\u9ad8\u6548\u6027\uff0c\u662f\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u5229\u5668\u3002\u5e0c\u671b\u540c\u5b66\u4eec\u80fd\u591f\u7ee7\u7eed\u6df1\u5165\u5b66\u4e60\u548c\u63a2\u7d22 BLAST+\uff0c\u5145\u5206\u5229\u7528\u8fd9\u4e00\u5de5\u5177\u5728\u5404\u81ea\u7684\u7814\u7a76\u9886\u57df\u4e2d\u6316\u6398\u66f4\u591a\u7684\u79d1\u5b66\u53d1\u73b0\u3002\u901a\u8fc7\u4e0d\u65ad\u5b9e\u8df5\u548c\u5e94\u7528\uff0c\u5927\u5bb6\u4e00\u5b9a\u80fd\u5728\u5206\u5b50\u751f\u7269\u5b66\u548c\u8fdb\u5316\u7814\u7a76\u4e2d\u53d6\u5f97\u66f4\u5927\u7684\u8fdb\u6b65\u548c\u6210\u679c\u3002<\/p>\n\n\n\n

\u540c\u5b66\u4eec\u5982\u679c\u89c9\u5f97\u81ea\u5df1\u5199\u4ee3\u7801\u9ebb\u70e6\uff0c\u53ef\u4ee5\u4f53\u9a8c\u4e00\u4e0b\u6211\u4eec\u7684\u4e91\u751f\u4fe1\u5c0f\u5de5\u5177\uff0c\u53ea\u9700\u8f93\u5165\u6570\u636e\uff0c\u5373\u53ef\u8f7b\u677e\u751f\u6210\u6240\u9700\u56fe\u8868\u3002\u7acb\u5373\u8bbf\u95ee\u4e91\u751f\u4fe1\uff08<\/a><\/strong>http:\/\/www.biocloudservice.com\/home.html<\/u><\/a>\uff09\uff0c\u5f00\u542f\u4fbf\u6377\u7684\u751f\u4fe1\u4e4b\u65c5\uff01<\/strong><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u540c\u5b66\u4eec\uff0c\u4eca\u5929\u5927\u6d77\u54e5\u8981\u5411\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e2a\u5728\u5206\u5b50\u751f\u7269\u5b66\u9886\u57df\u4e2d\u975e\u5e38\u91cd\u8981\u7684\u8f6f\u4ef6\u2014\u2014BLAST+\uff01BLAST+ \u662f\u4e00\u6b3e\u7528\u4e8e\u5e8f […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/61140"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=61140"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/61140\/revisions"}],"predecessor-version":[{"id":61148,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/61140\/revisions\/61148"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=61140"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=61140"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=61140"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}