{"id":61704,"date":"2024-11-19T15:31:50","date_gmt":"2024-11-19T07:31:50","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=61704"},"modified":"2024-11-19T15:31:50","modified_gmt":"2024-11-19T07:31:50","slug":"%e5%90%ab%e9%87%91%e9%87%8f%e8%b6%85%e9%ab%98%ef%bc%81muscle%e5%8c%85%ef%bc%8c%e5%a4%9a%e5%ba%8f%e5%88%97%e6%af%94%e5%af%b9%e9%a2%86%e5%9f%9f%e7%9a%84%e8%b6%85%e7%ba%a7%e7%a5%9e%e5%99%a8%ef%bc%8c","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=61704","title":{"rendered":"\u542b\u91d1\u91cf\u8d85\u9ad8\uff01muscle\u5305\uff0c\u591a\u5e8f\u5217\u6bd4\u5bf9\u9886\u57df\u7684\u8d85\u7ea7\u795e\u5668\uff0c\u4e00\u5206\u949f\u641e\u5b9a\u591a\u5e8f\u5217\u6bd4\u5bf9\uff01\uff01"},"content":{"rendered":"\n

<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u540c\u5b66\u4eec\uff0c\u5927\u5bb6\u597d\uff01\u4eca\u5929\u5c0f\u679c\u8981\u60f3\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e2a\u975e\u5e38\u5f3a\u5927\u7684\u591a\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\u2014\u2014muscle\u5305\u3002\u4f5c\u4e3a\u4e00\u6b3e\u9ad8\u6548\u7684\u591a\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\uff0cmuscle\u5305\u5728\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\u4e2d\u53d1\u6325\u7740\u91cd\u8981\u7684\u4f5c\u7528\u3002\u5b83\u80fd\u591f\u5bf9DNA\u3001RNA\u3001\u86cb\u767d\u8d28\u5e8f\u5217\u8fdb\u884c\u9ad8\u6548\u4e14\u7cbe\u786e\u7684\u6bd4\u5bf9\uff0c\u662f\u6211\u4eec\u5728\u5e8f\u5217\u5206\u6790\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u52a9\u624b\u3002<\/p>\n\n\n\n

muscle\u5305\u4e0d\u4ec5\u64cd\u4f5c\u7b80\u5355\uff0c\u800c\u4e14\u529f\u80fd\u5f3a\u5927\uff0c\u53ef\u4ee5\u5904\u7406\u5927\u91cf\u7684\u5e8f\u5217\u6570\u636e\uff0c\u8fdb\u884c\u590d\u6742\u7684\u6bd4\u5bf9\u4efb\u52a1\u3002\u66f4\u91cd\u8981\u7684\u662f\uff0cmuscle\u5305\u4e0eggmsa\u5305\u7684\u7ed3\u5408\uff0c\u4f7f\u5f97\u6211\u4eec\u53ef\u4ee5\u8f7b\u677e\u5730\u5c06\u6bd4\u5bf9\u7ed3\u679c\u8fdb\u884c\u53ef\u89c6\u5316\u5c55\u793a\uff0c\u8ba9\u590d\u6742\u7684\u751f\u7269\u6570\u636e\u4e00\u76ee\u4e86\u7136\u3002\u4efb\u4f55\u751f\u4fe1\u5206\u6790\u5bf9<\/strong>\u751f\u4fe1\u9ad8\u624b\u5c0f\u679c\u6765\u8bf4\u90fd\u4e0d\u662f\u96be\u9898<\/strong>\uff0c\u8981\u662f<\/strong>\u540c\u5b66\u4eec<\/strong>\u6709\u81ea\u5df1\u505a\u4e0d\u4e86\u7684\u751f\u4fe1\u5206\u6790\uff0c<\/strong>\u6b22\u8fce\u968f\u65f6<\/strong>\u8054\u7cfb\u6211<\/strong>\uff01\uff01\uff01<\/strong><\/strong><\/p>\n\n\n\n

\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u5c06\u4e00\u6b65\u6b65\u5b66\u4e60muscle\u5305\u7684\u5b89\u88c5\u65b9\u6cd5\u3001\u5982\u4f55\u8f7d\u5165\u793a\u4f8b\u6570\u636e\u3001\u8fdb\u884c\u591a\u5e8f\u5217\u6bd4\u5bf9\u4ee5\u53ca\u5229\u7528ggmsa\u5305\u8fdb\u884c\u7ed3\u679c\u7684\u53ef\u89c6\u5316\u5c55\u793a\u3002\u901a\u8fc7\u5b9e\u4f8b\u6f14\u793a\uff0c\u6211\u4eec\u5c06\u5bf931\u79cd\u54fa\u4e73\u52a8\u7269\u7684MAX\u57fa\u56e0\u5e8f\u5217\u8fdb\u884c\u6bd4\u5bf9\uff0c\u63ed\u793a\u5176\u5728\u4e0d\u540c\u7269\u79cd\u4e2d\u7684\u4fdd\u5b88\u6027\u548c\u53d8\u5f02\u60c5\u51b5\u3002\u8ba9\u6211\u4eec\u4e00\u8d77\u8e0f\u4e0a\u8fd9\u6bb5\u5b66\u4e60\u4e4b\u65c5\uff0c\u6df1\u5165\u63a2\u7d22muscle\u5305\u7684\u5f3a\u5927\u529f\u80fd\uff0c\u638c\u63e1\u591a\u5e8f\u5217\u6bd4\u5bf9\u7684\u6280\u5de7\uff0c\u63a8\u52a8\u81ea\u5df1\u7684\u7814\u7a76\u8fdb\u5c55\u3002\u51c6\u5907\u597d\u4e86\u5417\uff1f\u8ba9\u6211\u4eec\u5f00\u59cb\u5427\uff01<\/p>\n\n\n\n

\u672c\u6b21\u4ecb\u7ecd\u7684<\/strong>R\u5305<\/strong>\u9700\u8981\u8f83\u591a\u7684\u786c\u4ef6\u8d44\u6e90\uff0c\u5728<\/strong>\u670d\u52a1\u5668<\/strong>\u53ef\u4ee5\u66f4\u52a0\u6d41\u7545<\/strong>\u8fd0\u884c\uff0c<\/strong>\u540c\u5b66\u4eec<\/strong>\u5982\u679c\u6ca1\u6709\u81ea\u5df1\u7684\u670d\u52a1\u5668\u6b22\u8fce\u8054\u7cfb\u6211\u4eec<\/strong>\u8fdb\u884c<\/strong>\u670d\u52a1\u5668\u79df\u8d41~<\/strong><\/strong><\/p>\n\n\n\n

muscle\u5305\u4ecb\u7ecd<\/p>\n\n\n\n

muscle\u662f\u4e00\u4e2a\u63d0\u4f9b\u4e86\u5bf9Muscle\u591a\u5e8f\u5217\u6bd4\u5bf9\u7b97\u6cd5\u7684\u63a5\u53e3\u548c\u529f\u80fd\u7684R\u8bed\u8a00\u5305\u3002\u5b83\u5141\u8bb8\u7528\u6237\u5728R\u73af\u5883\u4e2d\u8f7b\u677e\u5730\u8fdb\u884c\u591a\u5e8f\u5217\u6bd4\u5bf9\u7684\u5206\u6790\u548c\u5904\u7406\u3002muscle\u5305\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u51fd\u6570\uff0c\u53ef\u4ee5\u65b9\u4fbf\u5730\u8c03\u7528Muscle\u7b97\u6cd5\u6267\u884c\u591a\u5e8f\u5217\u6bd4\u5bf9\uff0c\u5e76\u5c06\u7ed3\u679c\u4ee5\u9002\u5408R\u8bed\u8a00\u73af\u5883\u7684\u6570\u636e\u7ed3\u6784\u8fd4\u56de\uff0c\u4f7f\u7528\u6237\u80fd\u591f\u65b9\u4fbf\u5730\u8fdb\u884c\u540e\u7eed\u7684\u6570\u636e\u5206\u6790\u548c\u53ef\u89c6\u5316\u3002\u8be5\u5305\u8fd8\u63d0\u4f9b\u4e86\u4e30\u5bcc\u7684\u53c2\u6570\u9009\u9879\uff0c\u5141\u8bb8\u7528\u6237\u6839\u636e\u9700\u8981\u8c03\u6574\u6bd4\u5bf9\u7684\u7cbe\u5ea6\u548c\u901f\u5ea6\u3002\u901a\u8fc7muscle\u5305\uff0c\u7528\u6237\u53ef\u4ee5\u5728R\u4e2d\u7075\u6d3b\u5730\u8fdb\u884c\u591a\u5e8f\u5217\u6bd4\u5bf9\u7684\u7814\u7a76\u548c\u5206\u6790\uff0c\u4e3a\u751f\u7269\u4fe1\u606f\u5b66\u9886\u57df\u7684\u7814\u7a76\u63d0\u4f9b\u4e86\u4fbf\u5229\u7684\u5de5\u5177\u3002<\/p>\n\n\n\n

muscle\u5305\u7684\u5b89\u88c5<\/p>\n\n\n\n

\u9700\u8981R\u8bed\u8a00\u7248\u672c\u4e3a4.4\uff0c\u5728\u63a7\u5236\u53f0\u4e2d\u8f93\u5165\u4ee5\u4e0b\u547d\u4ee4\uff1a<\/p>\n\n\n\n

if (!require(“BiocManager”, quietly = TRUE))<\/p>\n\n\n\n

    install.packages(“BiocManager “)<\/p>\n\n\n\n

BiocManager::install(“muscle”) # \u5728BiocManager\u73af\u5883\u4e0b\u5b89\u88c5muscle<\/p>\n\n\n\n

\u67e5\u770b\u662f\u5426\u5b89\u88c5\u6210\u529f<\/p>\n\n\n\n

packageVersion(“muscle”) # \u67e5\u770bmuscle\u7248\u672c<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u663e\u793a\u4e3a3.46.0\u7248\u672c\uff0c\u5219\u8868\u793a\u5df2\u7ecf\u6210\u529f\u5b89\u88c5\u4e86muscle\u5305\u3002<\/p>\n\n\n\n

\u9664\u6b64\u4e4b\u5916\uff0c\u540e\u7eed\u793a\u4f8b\u8fd8\u9700\u8981\u4f7f\u7528ggmsa\u5305\uff0c\u6211\u4eec\u53ef\u4ee5\u63d0\u524d\u5b89\u88c5\uff0c\u5b89\u88c5\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

BiocManager::install(“ggmsa”) # \u5728BiocManager\u73af\u5883\u4e0b\u5b89\u88c5ggmsa<\/p>\n\n\n\n

packageVersion(“ggmsa”) # \u67e5\u770bggmsa\u7248\u672c<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u663e\u793a\u4e3a1.10.0\u7248\u672c\uff0c\u5219\u8868\u793a\u5df2\u7ecf\u6210\u529f\u5b89\u88c5\u4e86muscle\u5305\u3002<\/p>\n\n\n\n

\u81f3\u6b64\uff0c\u6211\u4eec\u5df2\u7ecf\u5b89\u88c5\u4e86\u6240\u6709\u9700\u8981\u7528\u5230\u7684R\u5305\uff0c\u63a5\u4e0b\u6765\u6211\u4eec\u5c31\u6b63\u5f0f\u5f00\u59cb\u5bf9muscle\u5305\u7684\u4ecb\u7ecd\uff0c\u5e72\u8d27\u6ee1\u6ee1\uff0c\u540c\u5b66\u4eec\u8981\u8ba4\u771f\u542c\u8bb2\u54e6\u3002<\/p>\n\n\n\n

\u4f7f\u7528muscle\u5305\u8fdb\u884c\u591a\u5e8f\u5217\u6bd4\u5bf9\u5e76\u53ef\u89c6\u5316<\/p>\n\n\n\n

\u5305\u548c\u6570\u636e\u7684\u8f7d\u5165\uff1a<\/strong><\/strong><\/p>\n\n\n\n

\u9996\u5148\u6211\u4eec\u9700\u8981\u8f7d\u5165muscle\u5305\uff0cggmsa\u5305\u5728\u540e\u7eed\u8fdb\u884c\u7ed3\u679c\u53ef\u89c6\u5316\u7684\u90e8\u5206\u9700\u8981\u7528\u5230\uff0c\u6211\u4eec\u53ef\u4ee5\u63d0\u524d\u8f7d\u5165\u3002\u9664\u6b64\u4e4b\u5916\u672c\u7bc7\u91c7\u7528\u7684\u793a\u4f8b\u6570\u636e\u4e3a31\u79cd\u54fa\u4e73\u52a8\u7269\u7684MAX\u57fa\u56e0\u5e8f\u5217\uff0c\u8be5\u793a\u4f8b\u6570\u636e\u6574\u5408\u5728muscle\u5305\u5185\uff0c\u6211\u4eec\u53ef\u4ee5\u76f4\u63a5\u8c03\u7528\u4f7f\u7528\uff0c\u76f8\u5173\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

# \u8f7d\u5165muscle\u5305<\/p>\n\n\n\n

library(muscle)<\/p>\n\n\n\n

# \u8f7d\u5165ggmsa\u5305<\/p>\n\n\n\n

library(ggmsa)<\/p>\n\n\n\n

# \u8f93\u51faumax\u5bf9\u8c61\u5185\u5bb9<\/p>\n\n\n\n

print(umax)<\/p>\n\n\n\n

# \u663e\u793aumax\u5bf9\u8c61\u7c7b\u522b<\/p>\n\n\n\n

class(umax)<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u663e\u793a\u5982\u4e0a\u56fe\uff0c\u53ef\u77e5umax\u5bf9\u8c61\u7c7b\u578b\u4e3aDNAStringSet\uff0c\u5171\u5305\u542b31\u6761MAX\u57fa\u56e0\u7684\u5e8f\u5217\u4fe1\u606f\uff0c\u8fd9\u4e9b\u57fa\u56e0\u7684\u5927\u81f4\u957f\u5ea6\u5728400-500bp\u4e4b\u95f4\uff0c\u6211\u4eec\u53ef\u4ee5\u6253\u5370\u5e8f\u5217\u7684\u540d\u79f0\uff0c\u67e5\u770b\u5176\u5305\u542b\u7684\u7269\u79cd\uff0c\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

# \u6253\u5370\u5e8f\u5217\u540d\u79f0<\/p>\n\n\n\n

names(umax)<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u663e\u793a\u5982\u4e0a\u56fe\uff0c\u53ef\u77e5umax\u6570\u636e\u96c6\u6db5\u76d6\u7684\u7269\u79cd\u4e30\u5bcc\uff0c\u5305\u62ec\u5927\u718a\u732b\uff08Ailuropoda_melanoleuca\uff09\u3001\u725b\uff08Bos_taurus \uff09\u3001\u4eba\u7c7b\uff08Homo_sapiens\uff09\u7b49\u5728\u5185\u768431\u4e2a\u7269\u79cd\u7684MAX\u57fa\u56e0\u3002<\/p>\n\n\n\n

\u4f7f\u7528muscle\u5305\u8fdb\u884c\u591a\u5e8f\u5217\u6bd4\u8f83<\/strong><\/strong><\/p>\n\n\n\n

\u6211\u4eec\u53ef\u4ee5\u76f4\u63a5\u4f7f\u7528muscle\u5305\u79cd\u7684muscle()\u51fd\u6570\uff0c\u8fdb\u884c\u591a\u5e8f\u5217\u6bd4\u8f83\uff0cmuscle()\u51fd\u6570\u7684\u53c2\u6570\u4e30\u5bcc\uff0c\u53ef\u4ee5\u6ee1\u8db3\u591a\u79cd\u5e8f\u5217\u6bd4\u5bf9\u7684\u9700\u8981\uff0c\u5982\u547d\u4ee4<\/p>\n\n\n\n

muscle(sequences\uff0cverbose= FALSE\uff0cgap_open= -400\uff0cgap_extend=-80)\u5176\u4e2d\uff1a<\/p>\n\n\n\n

verbose: \u4e00\u4e2a\u903b\u8f91\u503c\uff0c\u63a7\u5236\u662f\u5426\u8f93\u51fa\u989d\u5916\u7684\u4fe1\u606f\u6765\u663e\u793a\u6bd4\u5bf9\u7684\u8fdb\u5ea6\u548c\u7ec6\u8282\u3002\u9ed8\u8ba4\u4e3aFALSE\uff0c\u8868\u793a\u4e0d\u8f93\u51fa\u989d\u5916\u4fe1\u606f\u3002<\/p>\n\n\n\n

gap_open: \u8bbe\u7f6e\u5f00\u653e\u7f3a\u5931\u503c\u60e9\u7f5a\u7684\u503c\u3002\u8fd9\u662f\u4e00\u4e2a\u975e\u8d1f\u6570\uff0c\u7528\u4e8e\u60e9\u7f5a\u5728\u5e8f\u5217\u6bd4\u5bf9\u4e2d\u6253\u5f00\u4e00\u4e2a\u7f3a\u5931\u503c\u3002\u9ed8\u8ba4\u503c\u4e3a-400\u3002<\/p>\n\n\n\n

gap_extend: \u8bbe\u7f6e\u5ef6\u957f\u7f3a\u5931\u503c\u60e9\u7f5a\u7684\u503c\u3002\u8fd9\u662f\u4e00\u4e2a\u975e\u8d1f\u6570\uff0c\u7528\u4e8e\u60e9\u7f5a\u5728\u5e8f\u5217\u6bd4\u5bf9\u4e2d\u5ef6\u957f\u4e00\u4e2a\u5df2\u7ecf\u5b58\u5728\u7684\u7f3a\u5931\u503c\u3002\u9ed8\u8ba4\u503c\u4e3a-80\u3002<\/p>\n\n\n\n

\u4e3a\u4e86\u6f14\u793a\u7b80\u5355\u6613\u61c2\uff0c\u6211\u4eec\u4f7f\u7528muscle()\u51fd\u6570\u5747\u91c7\u7528\u9ed8\u8ba4\u53c2\u6570\uff0c\u76f8\u5173\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

#\u4f7f\u7528muscle()\u51fd\u6570\u5c06umax\u57fa\u56e0\u7c07\u4e2d\u7684\u57fa\u56e0\u8fdb\u884c\u591a\u5e8f\u5217\u6bd4\u5bf9<\/p>\n\n\n\n

aln <- muscle(umax, verbose= FALSE, gap_open= -400, gap_extend=-80)<\/p>\n\n\n\n

#\u6253\u5370\u6bd4\u5bf9\u7ed3\u679c<\/p>\n\n\n\n

aln<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u7ed3\u679c\u663e\u793a\u5982\u4e0a\u56fe\uff0c\u6211\u4eec\u53ef\u4ee5\u770b\u5230MAX\u57fa\u56e0\u76843\u2018\u7aef\u548c5\u2019\u7aef\u5747\u5177\u6709\u5927\u91cf\u7684\u4fdd\u5b88\u4f4d\u70b9\uff0c\u5728\u4e0d\u540c\u7269\u79cd\u4e2d\u5747\u5177\u6709\u76f8\u540c\u7684\u78b1\u57fa\u5e8f\u5217\uff0c\u4e3a\u4e86\u66f4\u597d\u7684\u5c55\u793a\u7ed3\u679c\uff0c\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528ggmsa\u5305\u6765\u7ed8\u5236DNA\u591a\u5e8f\u5217\u6bd4\u5bf9\u56fe\uff0c\u76f8\u5173\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

# \u8c03\u7528ggmsa\u51fd\u6570\u6765\u521b\u5efa\u591a\u5e8f\u5217\u6bd4\u5bf9\u7684\u56fe\u5f62\u5316\u8868\u793a<\/p>\n\n\n\n

# color: \u8bbe\u7f6e\u5e8f\u5217\u7684\u989c\u8272\u65b9\u6848\uff0c\u8fd9\u91cc\u4f7f\u7528\u4e86Zappo_AA\u989c\u8272\u65b9\u6848\uff0cfont: \u8bbe\u7f6e\u5b57\u4f53\uff0c\u8fd9\u91cc\u4f7f\u7528\u4e86DroidSansMono\u5b57\u4f53\uff0cchar_width: \u8bbe\u7f6e\u5b57\u7b26\u5bbd\u5ea6\uff0c\u63a7\u5236\u5e8f\u5217\u7684\u663e\u793a\u5bbd\u5ea6\uff0cseq_name: \u63a7\u5236\u662f\u5426\u663e\u793a\u5e8f\u5217\u540d\u79f0<\/p>\n\n\n\n

ggmsa(aln, color = “Zappo_AA”, font = “DroidSansMono”, char_width = 0.5, seq_name = TRUE ) +<\/p>\n\n\n\n

# \u6dfb\u52a0\u5e8f\u5217Logo\u56fe<\/p>\n\n\n\n

geom_seqlogo(color = “Zappo_AA”) +<\/p>\n\n\n\n

# \u6dfb\u52a0\u591a\u5e8f\u5217\u6bd4\u5bf9\u7684\u6807\u5c3a<\/p>\n\n\n\n

geom_msaBar()<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u7ed3\u679c\u5982\u4e0a\u56fe\uff0c\u53ef\u77e5MAX\u57fa\u56e0\u572831\u79cd\u54fa\u4e73\u52a8\u7269\u4e2d\u6574\u4f53\u5dee\u5f02\u8f83\u5c0f\uff0c\u53ea\u6709\u4e2a\u522b\u4f4d\u70b9\u53d1\u751f\u7a81\u53d8\uff0c\u8fd9\u8868\u660eMAX\u57fa\u56e0\u5728\u54fa\u4e73\u52a8\u7269\u4e2d\u53ef\u80fd\u5177\u6709\u9ad8\u5ea6\u4fdd\u5b88\u6027\uff0cMAX\u57fa\u56e0\u7f16\u7801\u4e00\u79cd\u53eb\u505aMAX\u7684\u86cb\u767d\uff0c\u5b83\u662f\u4e00\u79cd\u8f6c\u5f55\u56e0\u5b50\uff0c\u53c2\u4e0e\u8c03\u63a7\u7ec6\u80de\u751f\u957f\u548c\u5206\u5316\u7b49\u91cd\u8981\u751f\u7269\u5b66\u8fc7\u7a0b\uff0cMAX\u57fa\u56e0\u7684\u4fdd\u5b88\u6027\u610f\u5473\u7740MAX\u86cb\u767d\u5728\u4e0d\u540c\u54fa\u4e73\u52a8\u7269\u4e2d\u7684\u529f\u80fd\u9700\u6c42\u7c7b\u4f3c\uff0c\u56e0\u6b64\u57fa\u56e0\u5e8f\u5217\u4fdd\u6301\u76f8\u5bf9\u7a33\u5b9a\u3002\u4e2a\u522b\u4f4d\u70b9\u7684\u7a81\u53d8\u53ef\u80fd\u662f\u7531\u4e8e\u4e2a\u4f53\u95f4\u7684\u9057\u4f20\u53d8\u5f02\u6216\u73af\u5883\u538b\u529b\u7b49\u56e0\u7d20\u5bfc\u81f4\u7684\uff0c\u8fd9\u4e9b\u7a81\u53d8\u53ef\u80fd\u5bf9MAX\u57fa\u56e0\u7684\u529f\u80fd\u4ea7\u751f\u4e00\u5b9a\u5f71\u54cd\uff0c\u4f46\u7531\u4e8e\u6574\u4f53\u4e0a\u4fdd\u5b88\u6027\u8f83\u9ad8\uff0c\u8fd9\u4e9b\u5f71\u54cd\u53ef\u80fd\u5e76\u4e0d\u4f1a\u5bfc\u81f4\u57fa\u56e0\u529f\u80fd\u7684\u663e\u8457\u6539\u53d8\u3002\u540c\u5b66\u4eec\u53ef\u4ee5\u7ed3\u5408\u81ea\u5df1\u8bfe\u9898\u7684\u5e8f\u5217\u4fe1\u606f\uff0c\u5bf9\u591a\u5e8f\u5217\u6bd4\u5bf9\u7ed3\u679c\u8fdb\u884c\u89e3\u91ca\u8bf4\u660e\u3002<\/p>\n\n\n\n

\u4ee5\u4e0a\u5c31\u662f\u5bf9\u4e8emuscle\u5305\u7684\u5168\u90e8\u4ecb\u7ecd\u4e86\uff0c\u5728\u672c\u6587\u4e2d\uff0c\u6211\u4eec\u8be6\u7ec6\u4ecb\u7ecd\u4e86muscle\u5305\u5728R\u8bed\u8a00\u73af\u5883\u4e0b\u7684\u5b89\u88c5\u548c\u4f7f\u7528\u65b9\u6cd5\uff0c\u5e76\u901a\u8fc7\u5b9e\u4f8b\u6f14\u793a\u4e86\u5982\u4f55\u5229\u7528muscle\u5305\u5bf931\u79cd\u54fa\u4e73\u52a8\u7269\u7684MAX\u57fa\u56e0\u5e8f\u5217\u8fdb\u884c\u591a\u5e8f\u5217\u6bd4\u5bf9\u3002\u901a\u8fc7\u672c\u6587\u7684\u4ecb\u7ecd\uff0c\u540c\u5b66\u4eec\u4e0d\u4ec5\u4e86\u89e3\u4e86muscle\u5305\u7684\u57fa\u672c\u4f7f\u7528\u65b9\u6cd5\uff0c\u8fd8\u638c\u63e1\u4e86\u5982\u4f55\u7ed3\u5408ggmsa\u5305\u8fdb\u884c\u7ed3\u679c\u7684\u53ef\u89c6\u5316\u5c55\u793a\u3002\u8fd9\u4e9b\u6280\u80fd\u5bf9\u4e8e\u5206\u6790\u548c\u89e3\u91ca\u591a\u5e8f\u5217\u6bd4\u5bf9\u7ed3\u679c\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002\u5e0c\u671b\u540c\u5b66\u4eec\u5728\u4eca\u540e\u7684\u5b66\u4e60\u548c\u7814\u7a76\u4e2d\uff0c\u80fd\u591f\u7075\u6d3b\u5e94\u7528muscle\u5305\u53ca\u76f8\u5173\u5de5\u5177\uff0c\u6df1\u5165\u5206\u6790\u751f\u7269\u5e8f\u5217\u6570\u636e\uff0c\u63a8\u52a8\u81ea\u5df1\u7684\u7814\u7a76\u8fdb\u5c55\u3002\u7ee7\u7eed\u52aa\u529b\u5b66\u4e60muscle\u5305\uff0c\u76f8\u4fe1\u4f60\u4eec\u4f1a\u53d1\u73b0\u66f4\u591a\u7684\u5e94\u7528\u573a\u666f\u548c\u6f5c\u5728\u7684\u7814\u7a76\u4ef7\u503c\u3002<\/p>\n\n\n\n

\u540c\u5b66\u4eec\u5982\u679c\u89c9\u5f97\u81ea\u5df1\u5199\u4ee3\u7801\u9ebb\u70e6\uff0c\u53ef\u4ee5\u4f53\u9a8c\u4e00\u4e0b\u6211\u4eec\u7684\u4e91\u751f\u4fe1\u5c0f\u5de5\u5177\uff0c\u53ea\u9700\u8f93\u5165\u6570\u636e\uff0c\u5373\u53ef\u8f7b\u677e\u751f\u6210\u6240\u9700\u56fe\u8868\u3002\u7acb\u5373\u8bbf\u95ee\u4e91\u751f\u4fe1\uff08<\/a><\/strong>http:\/\/www.biocloudservice.com\/home.html<\/u><\/a>\uff09\uff0c\u5f00\u542f\u4fbf\u6377\u7684\u751f\u4fe1\u4e4b\u65c5\uff01<\/strong><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u540c\u5b66\u4eec\uff0c\u5927\u5bb6\u597d\uff01\u4eca\u5929\u5c0f\u679c\u8981\u60f3\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e2a\u975e\u5e38\u5f3a\u5927\u7684\u591a\u5e8f\u5217\u6bd4\u5bf9\u5de5\u5177\u2014\u2014muscle\u5305\u3002\u4f5c\u4e3a\u4e00\u6b3e\u9ad8\u6548\u7684\u591a\u5e8f\u5217\u6bd4\u5bf9\u5de5 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/61704"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=61704"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/61704\/revisions"}],"predecessor-version":[{"id":61712,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/61704\/revisions\/61712"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=61704"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=61704"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=61704"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}