{"id":61751,"date":"2024-11-19T15:28:40","date_gmt":"2024-11-19T07:28:40","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=61751"},"modified":"2024-11-19T15:28:40","modified_gmt":"2024-11-19T07:28:40","slug":"%e6%8f%ad%e7%a7%98epicv2%e9%98%b5%e5%88%97%ef%bc%9admrcate%e5%b8%a6%e4%bd%a0%e6%8e%a2%e5%af%bb%e5%b7%ae%e5%bc%82%e7%94%b2%e5%9f%ba%e5%8c%96%e5%8c%ba%e5%9f%9f%ef%bc%81","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=61751","title":{"rendered":"\u63ed\u79d8EPICv2\u9635\u5217\uff1aDMRcate\u5e26\u4f60\u63a2\u5bfb\u5dee\u5f02\u7532\u57fa\u5316\u533a\u57df\uff01"},"content":{"rendered":"\n

<\/p>\n\n\n\n

<\/a>\u563f\uff0c\u5927\u5bb6\u597d\uff0c\u6b22\u8fce\u4f19\u4f34\u4eec\u8ddf\u7740\u5c0f\u679c\u4e00\u8d77\u63a2\u7d22\u751f\u7269\u4fe1\u606f\u5b66\u7684\u5947\u5999\u4e16\u754c\u3002\u4eca\u5929\uff0c<\/a>\u5c0f\u679c\u5c06\u5e26\u9886\u5927\u5bb6\u4e00\u8d77\u5728DNA\u7532\u57fa\u5316\u5206\u6790\u4e2d\u63a2\u5bfb\u57fa\u56e0\u7684\u5965\u79d8\uff0c\u8ba9\u6211\u4eec\u4e00\u8d77\u770b\u4e0b\u53bb\u5427~<\/p>\n\n\n\n

\u5728\u8fc7\u53bb\u768415\u5e74\u4e2d\uff0cInfinium\u7532\u57fa\u5316\u73e0\u7247\u5fae\u9635\u5217\u5df2\u5e7f\u6cdb\u7528\u4e8e\u5168\u57fa\u56e0\u7ec4DNA\u7532\u57fa\u5316\u5206\u6790\u3002\u7ee7\u6d41\u884c\u7684EPICv1\u4e4b\u540e\uff0c\u65b0\u53d1\u5e03\u7684EPICv2\u58f0\u79f0\u53ef\u4ee5\u5c06\u57fa\u56e0\u7ec4\u8986\u76d6\u8303\u56f4\u6269\u5c55\u5230\u8d85\u8fc7935,000\u4e2aCpG\u4f4d\u70b9\u3002\u4eca\u5929\uff0c\u6211\u4eec\u8981\u4e00\u8d77\u63a2\u7d22\u4e00\u4e2a\u5b9e\u6218\u6848\u4f8b\uff0c\u5728B\u7ec6\u80de\u6025\u6027\u6dcb\u5df4\u7ec6\u80de\u767d\u8840\u75c5\uff08ALL\uff09\u548cT\u7ec6\u80deALL\u6837\u672c\u95f4\u5bfb\u627eEPICv2\u9635\u5217\u4e2d\u7684\u5dee\u5f02\u7532\u57fa\u5316\u533a\u57df\uff08DMRs\uff09\u3002<\/p>\n\n\n\n

<\/a>\u5728\u8fd9\u4e2a\u6848\u4f8b\u4e2d\uff0c\u6211\u4eec\u5c06\u4f7f\u7528DMRcate\u5305\uff0c\u5b83\u662f\u4e00\u4e2a\u5f3a\u5927\u7684\u5de5\u5177\uff0c\u53ef\u4ee5\u5e2e\u52a9\u6211\u4eec\u5728\u7532\u57fa\u5316\u6570\u636e\u4e2d\u627e\u5230\u90a3\u4e9b\u5dee\u5f02\u7532\u57fa\u5316\u533a\u57df\uff08DMRs\uff09\uff0c\u5c31\u597d\u50cf\u627e\u5230\u4e86\u57fa\u56e0\u7684\u201c\u6307\u7eb9\u201d\uff0c\u4ece\u800c\u63ed\u793a\u51faB\u7ec6\u80de\u548cT\u7ec6\u80de\u6025\u6027\u6dcb\u5df4\u7ec6\u80de\u767d\u8840\u75c5\u6837\u672c\u95f4\u7684\u72ec\u7279\u5dee\u5f02\uff0c\u4e3a\u6211\u4eec\u7684\u7814\u7a76\u63d0\u4f9b\u91cd\u8981\u7684\u751f\u7269\u5b66\u89c1\u89e3\u3002\u5c0f\u679c\u63d0\u9192\u4e00\u53e5\uff0c\u8fd9\u4e2a<\/strong>R\u5305\u64cd\u4f5c\u5360\u7528\u5185\u5b58\u6bd4\u8f83\u5927\uff0c\u5efa\u8bae\u4f7f\u7528\u670d\u52a1\u5668<\/strong>\u54e6<\/strong>\uff0c\u6b22\u8fce\u8054\u7cfb\u5c0f\u679c\u79df\u8d41\u6027\u4ef7\u6bd4\u9ad8\u7684\u670d\u52a1\u5668<\/strong>~<\/strong><\/p>\n\n\n\n

\u63a5\u4e0b\u6765\uff0c\u5c0f\u679c\u5c06\u5e26\u9886\u5927\u5bb6\u4e00\u6b65\u6b65\u8fdb\u884c\u64cd\u4f5c\uff0c\u4ece\u6570\u636e\u9884\u5904\u7406\uff0c\u5230DMRs\u7684\u5bfb\u627e\uff0c\u518d\u5230\u7ed3\u679c\u7684\u89e3\u8bfb\uff0c\u6211\u4eec\u5c06\u4e00\u8d77\u63ed\u5f00\u8fd9\u4e2a\u6848\u4f8b\u7684\u795e\u79d8\u9762\u7eb1\u3002\u5982\u679c\u4f60\u5728\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u9047\u5230\u4efb\u4f55\u95ee\u9898\uff0c\u522b\u72b9\u8c6b\uff0c\u8bb0\u5f97\u8054\u7cfb\u5c0f\u679c\u54e6~<\/strong><\/strong><\/p>\n\n\n\n

\u6211\u4eec\u5148\u5b89\u88c5\u5e76\u52a0\u8f7dDMRcate\u5305\uff1a<\/p>\n\n\n\n

if (!require(“BiocManager”))<\/p>\n\n\n\n

  install.packages(“BiocManager”)<\/p>\n\n\n\n

BiocManager::install(“DMRcate”)<\/p>\n\n\n\n

library(DMRcate)<\/p>\n\n\n\n

\u7b2c\u4e00\u6b65\uff0c\u83b7\u53d6\u6570\u636e\u3002\u6211\u4eec\u4f7f\u7528\u6765\u81ea Noguera-Castells \u7b49\u4eba\u7684\u6837\u672c\u5b50\u96c6\u6765\u67e5\u627e T \u7ec6\u80de\u6025\u6027\u6dcb\u5df4\u7ec6\u80de\u767d\u8840\u75c5 (TALL) \u548c B \u7ec6\u80de\u6025\u6027\u6dcb\u5df4\u7ec6\u80de\u767d\u8840\u75c5 (BALL) \u4e4b\u95f4\u7684 DMR\u3002\u6211\u4eec\u4ece ExperimentHub \u52a0\u8f7d beta \u77e9\u9635\uff1a<\/p>\n\n\n\n

library(ExperimentHub)<\/p>\n\n\n\n

# \u8bbe\u7f6e\u4ee3\u7406<\/p>\n\n\n\n

#proxy <- httr::use_proxy(“http:\/\/my_user:my_password@myproxy:8080”)<\/p>\n\n\n\n

## or<\/p>\n\n\n\n

proxy <- httr::use_proxy(Sys.getenv(‘http_proxy’))<\/p>\n\n\n\n

httr::set_config(proxy)<\/p>\n\n\n\n

ExperimentHub::setExperimentHubOption(“PROXY”, proxy)<\/p>\n\n\n\n

# \u83b7\u53d6\u6570\u636e<\/p>\n\n\n\n

eh <- ExperimentHub()<\/p>\n\n\n\n

ALLbetas <- eh[[“EH9451”]]<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u5728\u8f93\u51fa\u4e2d\u6211\u4eec\u53ef\u4ee5\u770b\u5230\uff0cALLbetas\u6570\u636e\u4e2d\u603b\u5171\u670910\u5217\u6570\u636e\uff0c\u6bcf\u4e00\u5217\u4ee3\u8868\u4e00\u4e2a\u6837\u672c\u7684beta\u503c\uff0c\u8fd910\u5217\u5206\u522b\u4ee3\u8868 5 \u4e2a TALL \u6837\u672c\u548c 5 \u4e2a BALL \u6837\u672c\u3002<\/p>\n\n\n\n

\u63a5\u4e0b\u6765\uff0c\u8ba9\u6211\u4eec\u7ed8\u5236\u4e00\u4e2a\u5bc6\u5ea6\u56fe\uff0c\u770b\u4e00\u4e0bALLbetas\u6570\u636e\u96c6\u4e2d\u7684\u6570\u636e\u5206\u5e03\u5427~ \u8ddf\u7740\u5c0f\u679c\u4e00\u8d77\u8f93\u5165\u4ee5\u4e0b\u4ee3\u7801\uff1a<\/p>\n\n\n\n

plot(density(ALLbetas[,1]), col=”forestgreen”, xlab=”Beta value”, ylim=c(0, 6),<\/p>\n\n\n\n

     main=”Noguera-Castells et al. 2023 ALL samples”, lwd=2)<\/p>\n\n\n\n

invisible(sapply(2:5, function (x) lines(density(ALLbetas[,x]), col=”forestgreen”, lwd=2)))<\/p>\n\n\n\n

invisible(sapply(6:10, function (x) lines(density(ALLbetas[,x]), col=”orange”, lwd=2)))<\/p>\n\n\n\n

legend(“topleft”, c(“B cell ALL”, “T cell ALL”), text.col=c(“forestgreen”, “orange”))<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u4ece\u5bc6\u5ea6\u5206\u5e03\u56fe\u4e2d\u53ef\u4ee5\u770b\u51fa\uff0cB\u7ec6\u80deALL\u548cT\u7ec6\u80deALL\u7684\u7532\u57fa\u5316\u5206\u5e03\u6709\u6240\u4e0d\u540c\u3002\u8fd9\u53ef\u80fd\u610f\u5473\u7740\u8fd9\u4e24\u79cd\u7c7b\u578b\u7684\u767d\u8840\u75c5\u5728\u7532\u57fa\u5316\u6c34\u5e73\u4e0a\u6709\u6240\u533a\u522b\uff0c\u8fd9\u53ef\u80fd\u4e0e\u5b83\u4eec\u7684\u751f\u7269\u5b66\u7279\u6027\u548c\u4e34\u5e8a\u8868\u73b0\u6709\u5173\u3002\u7136\u800c\uff0c\u8fd9\u53ea\u662f\u4e00\u4e2a\u521d\u6b65\u7684\u89c2\u5bdf\uff0c\u6211\u4eec\u9700\u8981\u8fdb\u4e00\u6b65\u7684\u7edf\u8ba1\u5206\u6790\u6765\u8bc1\u5b9e\u8fd9\u4e00\u70b9\u3002<\/p>\n\n\n\n

\u73b0\u5728\u8ba9\u6211\u4eec\u5c06 beta \u503c\u8fdb\u884c logit2 \u53d8\u6362\uff0c\u5f97\u5230M\u503c\u3002\u8fd9\u662f\u56e0\u4e3aM\u503c\u8fd1\u4f3c\u4e8e\u6b63\u6001\u5206\u5e03\uff0c\u66f4\u9002\u5408\u8fdb\u884c\u7ebf\u6027\u5efa\u6a21\u3002<\/p>\n\n\n\n

ALLMs <- minfi::logit2(ALLbetas)<\/p>\n\n\n\n

\u7136\u540e\uff0c\u6253\u5370\u51fa\u8f6c\u6362\u540e\u7684\u6570\u636e\u96c6ALLMs\u7684\u884c\u6570\uff0c\u4e5f\u5c31\u662f\u5f97\u5230\u63a2\u9488\u7684\u6570\u91cf\u4e3a894902\u3002<\/p>\n\n\n\n

nrow(ALLMs)<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u63a5\u7740\uff0c\u4f7f\u7528rmSNPandCH\u51fd\u6570\u8fc7\u6ee4\u6389\u9760\u8fd1SNP\u7684\u63a2\u9488\u3002\u8fd9\u91cc\u7684rmcrosshyb = FALSE\u53c2\u6570\u8868\u793a\u4fdd\u7559\u90a3\u4e9b\u53ef\u80fd\u4e0e\u57fa\u56e0\u7ec4\u5176\u4ed6\u90e8\u5206\u53d1\u751f\u4ea4\u53c9\u53cd\u5e94\u7684\u63a2\u9488\u3002<\/p>\n\n\n\n

ALLMs.noSNPs <- rmSNPandCH(ALLMs, rmcrosshyb = FALSE)<\/p>\n\n\n\n

\u6700\u540e\uff0c\u6253\u5370\u51fa\u8fc7\u6ee4\u540e\u7684\u6570\u636e\u96c6ALLMs.noSNPs\u7684\u884c\u6570\uff0c\u5f97\u5230\u8fc7\u6ee4\u540e\u5269\u4f59\u7684\u63a2\u9488\u6570\u91cf\u4e3a893321\u3002<\/p>\n\n\n\n

nrow(ALLMs.noSNPs)<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u4ece\u8f93\u51fa\u7ed3\u679c\u53ef\u4ee5\u770b\u51fa\uff0c\u8fc7\u6ee4\u540e\u7684\u63a2\u9488\u6570\u91cf\u6bd4\u8fc7\u6ee4\u524d\u5c11\u4e861581\u4e2a\uff0c\u8fd9\u4e9b\u88ab\u8fc7\u6ee4\u6389\u7684\u63a2\u9488\u53ef\u80fd\u662f\u9760\u8fd1SNP\u7684\u63a2\u9488\u3002<\/p>\n\n\n\n

\u4e0e\u5148\u524d\u7684\u7248\u672cEPICv1\u76f8\u6bd4\uff0cEPICv2\u7684\u65b0\u7279\u6027\u4e4b\u4e00\u662f\u5b83\u4f1a\u6709\u591a\u4e2a\u63a2\u9488\u6620\u5c04\u5230\u540c\u4e00CpG\u4f4d\u70b9\u3002\u8fd9\u4e00\u7279\u6027\u5728\u63a2\u9488\u7684\u6027\u80fd\u6d4b\u8bd5\u4e0a\u975e\u5e38\u6709\u7528\uff0c\u4f46\u5bf9\u4e8eDMRcate\u5219\u66f4\u5e0c\u671b\u63a2\u9488\u5230CpG\u4f4d\u70b9\u662f\u4e00\u5bf9\u4e00\u6620\u5c04\u7684\uff0c\u5426\u5219\u6838\u5fc3\u5c06\u504f\u5411\u90a3\u4e9b\u5177\u6709\u66f4\u591a\u590d\u5236\u54c1\u7684\u4f4d\u70b9\u3002<\/p>\n\n\n\n

rmPosReps()\u51fd\u6570\u5c31\u53ef\u4ee5\u8c03\u6574M\u503c\u77e9\u9635\u4ee5\u7b26\u5408\u6211\u4eec\u7684\u8981\u6c42\u3002\u6211\u4eec\u53ef\u4ee5\u901a\u8fc7`filter.strategy`\u53c2\u6570\u6765\u6307\u5b9a\u9009\u9879\u3002\u9ed8\u8ba4\u60c5\u51b5\u4e0b\uff0c\u5b83\u4f1a\u53d6\u6bcf\u4e2a\u590d\u5236\u7ec4\u7684\u5e73\u5747\u503c\u3002<\/p>\n\n\n\n

AnnotationHub::setAnnotationHubOption(“PROXY”, proxy)<\/p>\n\n\n\n

ALLMs.noSNPs.repmean <- rmPosReps(ALLMs.noSNPs, filter.strategy=”mean”)<\/p>\n\n\n\n

filter.strategy\u9664\u4e86\u53d6\u5e73\u5747\u503c\u7684\u65b9\u6cd5\uff0c\u8fd8\u63d0\u4f9b\u4e86\u591a\u79cd\u9009\u62e9\u7b56\u7565\uff0c\u5982\u654f\u611f\u5ea6\u3001\u7cbe\u5ea6\u6216\u8005\u968f\u673a\u9009\u62e9\u3002\u4f7f\u7528\u4e0d\u540c\u7684\u53c2\u6570\uff0c\u63a2\u9488\u9009\u62e9\u4e5f\u4f1a\u53d1\u751f\u53d8\u5316\u3002\u4e0b\u56fe\u663e\u793a\u4e86\u57fa\u4e8efilter.strategy\u53c2\u6570\u7684\u63a2\u9488\u9009\u62e9\u7684\u5dee\u5f02\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u5b9e\u9645\u4e0a\uff0c\u4e0d\u540c\u7532\u57fa\u5316CpG\u4f4d\u70b9\u7684\u6570\u91cf\u4e00\u822c\u4e0d\u592a\u4f1a\u56e0\u4e3afilter.strategy\u53c2\u6570\u7684\u4e0d\u540c\u800c\u6709\u5f88\u5927\u53d8\u5316\uff0c\u4f46filter.strategy\u63d0\u4f9b\u8fd9\u79cd\u9009\u62e9\u6027\uff0c\u4f1a\u6709\u52a9\u4e8e\u6211\u4eec\u5229\u7528\u9635\u5217\u4e0a\u7684\u6240\u6709\u53ef\u7528\u63a2\u9488\u3002<\/p>\n\n\n\n

\u63a5\u4e0b\u6765\uff0c\u8ba9\u6211\u4eec\u8fdb\u5165\u672c\u6b21\u6848\u4f8b\u6700\u91cd\u8981\u7684\u90e8\u5206 \u2014\u2014 \u63a2\u5bfb\u5dee\u5f02\u7532\u57fa\u5316\u533a\u57df\uff08DMRs\uff09\uff01<\/p>\n\n\n\n

\u9996\u5148\uff0c\u4f7f\u7528gsub\u51fd\u6570\u4eceALLMs.noSNPs.repmean\u7684\u5217\u540d\u4e2d\u63d0\u53d6\u6837\u672c\u7c7b\u578b\u3002\u53ef\u4ee5\u770b\u5230type\u662f\u4e00\u4e2a\u5305\u542b\u6837\u672c\u7c7b\u578b\uff08”BALL”\u6216”TALL”\uff09\u7684\u5411\u91cf\u3002<\/p>\n\n\n\n

type <- gsub(“_.*”, “”, colnames(ALLMs.noSNPs.repmean))<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u63a5\u7740\uff0c\u6211\u4eec\u521b\u5efa\u4e00\u4e2a\u8bbe\u8ba1\u77e9\u9635\uff0c\u7528\u4e8e\u540e\u7eed\u7684\u7ebf\u6027\u6a21\u578b\u5206\u6790\u3002PS\uff1a\u8bbe\u8ba1\u77e9\u9635\u662f\u4e00\u79cd\u5e38\u7528\u4e8e\u7edf\u8ba1\u5206\u6790\u548c\u673a\u5668\u5b66\u4e60\u7684\u6570\u636e\u7ed3\u6784\uff0c\u5b83\u5c06\u89e3\u91ca\u53d8\u91cf\uff08\u5728\u8fd9\u91cc\u662f\u6837\u672c\u7c7b\u578b\uff09\u7f16\u7801\u4e3a\u4e00\u7ec4\u53ef\u4ee5\u8f93\u5165\u5230\u6a21\u578b\u4e2d\u7684\u6570\u503c\u3002<\/p>\n\n\n\n

design <- model.matrix(~type)<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u73b0\u5728\u8ba9\u6211\u4eec\u6765\u770b\u770b\u7528\u4e8e\u6ce8\u91caCpG\u4f4d\u70b9\u7684\u51fd\u6570<\/a>\u2014\u2014cpg.annotate()\u3002\u5728\u8fd9\u4e2a\u51fd\u6570\u4e2d\uff0c\u903b\u8f91\u53c2\u6570epicv2Remap\u80fd\u5141\u8bb8\u6211\u4eec\u5728\u6709\u8bc1\u636e\u663e\u793a\u63a2\u9488\u66f4\u503e\u5411\u4e8e\u4e0e\u8131\u9776CpG\u4f4d\u70b9\u6742\u4ea4\u7684\u60c5\u51b5\u4e0b\uff0c\u5c06\u63a2\u9488\u91cd\u65b0\u5206\u914d\u5230\u8be5\u4f4d\u70b9\u3002\u5176\u4e2d\u63d0\u5230\u7684\u8bc1\u636e\u4e00\u822c\u6765\u81ea\u4e8e\u57fa\u56e0\u7ec4\u6bd4\u5bf9\u548cWGBS\u6570\u636e\u7684\u4e00\u81f4\u6027\u3002\u5f53\u7136\uff0c\u8fd9\u4ec5\u9002\u7528\u4e8e\u5728rmSNPandCH()\u4e2d\u6307\u5b9a\u4e86rmcrosshyb=FALSE\u7684\u60c5\u51b5\u3002<\/p>\n\n\n\n

myannotation <- cpg.annotate(“array”, object=ALLMs.noSNPs.repmean, what = “M”,<\/p>\n\n\n\n

                             arraytype = “EPICv2”, epicv2Remap = TRUE,<\/p>\n\n\n\n

                             analysis.type=”differential”, design=design, coef=2,<\/p>\n\n\n\n

                             fdr=0.001)<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u8f93\u51fa\u663e\u793a\uff0c\u5728\u6211\u4eec\u7684\u6848\u4f8b\u4e2d\u8fd4\u56de\u4e8629184\u4e2a\u5355\u72ec\u663e\u8457\u7684\u63a2\u9488\u3002<\/p>\n\n\n\n

\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528dmrcate()\u51fd\u6570\u6765\u8c03\u7528\u5dee\u5f02\u7532\u57fa\u5316\u533a\u57df\uff08DMRs\uff09\u5e76\u5bf9\u5b83\u4eec\u8fdb\u884c\u6ce8\u91ca\u3002\u9700\u8981\u6ce8\u610f\u7684\u662f\uff0c\u4e0e\u4e4b\u524d\u7684\u9635\u5217\u4e0d\u540c\uff0cEPICv2\u9635\u5217\u7684\u6ce8\u91ca\u662f\u57fa\u4e8ehg38\u7248\u672c\u7684\u4eba\u7c7b\u57fa\u56e0\u7ec4\u7684\u3002<\/p>\n\n\n\n

dmrcoutput <- dmrcate(myannotation, lambda=1000, C=2)<\/p>\n\n\n\n

results.ranges <- extractRanges(dmrcoutput, genome = “hg38”)<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u6700\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528DMR.plot()\u51fd\u6570\u6765\u7ed8\u5236\u6700\u91cd\u8981\u7684\u5dee\u5f02\u7532\u57fa\u5316\u533a\u57df\uff08DMRs\uff09\u4e4b\u4e00\u3002<\/p>\n\n\n\n

groups <- c(BALL=”forestgreen”, TALL=”orange”)<\/p>\n\n\n\n

cols <- groups[as.character(type)]<\/p>\n\n\n\n

DMR.plot(ranges=results.ranges, dmr=2, CpGs=ALLbetas,<\/p>\n\n\n\n

         what = “Beta”, arraytype = “EPICv2”, phen.col=cols, genome = “hg38”)<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe\u4e2d\u5c55\u793a\u4e86\u7279\u5b9aDMR\u7684\u7532\u57fa\u5316\u6c34\u5e73\uff0c\u56fe\u7531\u4e09\u90e8\u5206\u7ec4\u6210\uff1a\u9876\u90e8\u663e\u793aDMR\u5728\u7b2c11\u53f7\u67d3\u8272\u4f53\u7684\u4f4d\u7f6e\uff0c\u4e2d\u95f4\u90e8\u5206\u5c55\u793a\u6bcf\u4e2a\u4e2a\u4f53\u5728DMR\u7684CpG\u4f4d\u70b9\u7684\u7532\u57fa\u5316\u6c34\u5e73\uff0c\u5e95\u90e8\u663e\u793a\u6bcf\u4e2a\u7ec4\u7684\u5e73\u5747\u7532\u57fa\u5316\u6c34\u5e73\u3002\u56fe\u5206\u6790\u8868\u660e\uff0cDMR\u53ef\u80fd\u4e0e\u4e24\u7ec4\u7684\u8868\u578b\u5dee\u5f02\u6709\u5173\uff0c\u9700\u8981\u8fdb\u4e00\u6b65\u7814\u7a76\u3002<\/p>\n\n\n\n

\u4f19\u4f34\u4eec\uff0c\u5c0f\u679c\u5df2\u7ecf\u5e26\u9886\u5927\u5bb6\u4e00\u8d77\u5b8c\u6210\u4e86DMRcate\u7684\u521d\u6b65\u63a2\u7d22\u3002\u73b0\u5728\uff0c\u4f60\u53ef\u4ee5\u5f00\u59cb\u81ea\u5df1\u52a8\u624b\uff0c\u5229\u7528DMRcate<\/a>\u5305\u6765\u5206\u6790\u5dee\u5f02\u7532\u57fa\u5316\u533a\u57df\uff08DMRs\uff09\u5566~<\/p>\n\n\n\n

\u65e0\u8bba\u4f60\u662f\u5728\u4f18\u5316\u4ee3\u7801\uff0c\u8fd8\u662f\u5728\u4e91\u7aef\u8fdb\u884c\u4fbf\u6377\u7684\u5206\u6790\uff0c\u4e91\u751f\u4fe1\u795e\u5668\u90fd\u80fd\u4e3a\u4f60\u63d0\u4f9b\u5f3a\u5927\u7684\u652f\u6301\u3002\u6b22\u8fce<\/strong>\u8bd5\u8bd5\u6211\u4eec\u7684\u4e91\u751f\u4fe1\u795e\u5668<\/strong>\uff0c<\/strong>\u53ea\u9700<\/strong>\u4e00\u952e<\/strong>\u4e0a\u4f20\u6570\u636e\uff0c\u60f3\u8981\u7684\u56fe\u5c31\u80fd\u8f7b\u677e<\/strong>get~<\/strong><\/strong><\/p>\n\n\n\n

\u4e91\u751f\u4fe1\u5e73\u53f0\u94fe\u63a5\uff1a<\/strong>http:\/\/www.biocloudservice.com\/home.html<\/strong><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u563f\uff0c\u5927\u5bb6\u597d\uff0c\u6b22\u8fce\u4f19\u4f34\u4eec\u8ddf\u7740\u5c0f\u679c\u4e00\u8d77\u63a2\u7d22\u751f\u7269\u4fe1\u606f\u5b66\u7684\u5947\u5999\u4e16\u754c\u3002\u4eca\u5929\uff0c\u5c0f\u679c\u5c06\u5e26\u9886\u5927\u5bb6\u4e00\u8d77\u5728DNA\u7532\u57fa\u5316\u5206\u6790\u4e2d\u63a2\u5bfb\u57fa […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":""},"categories":[1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/61751"}],"collection":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=61751"}],"version-history":[{"count":1,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/61751\/revisions"}],"predecessor-version":[{"id":61762,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/61751\/revisions\/61762"}],"wp:attachment":[{"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=61751"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=61751"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.biocloudservice.com\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=61751"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}