{"id":61823,"date":"2024-11-21T15:38:11","date_gmt":"2024-11-21T07:38:11","guid":{"rendered":"http:\/\/www.biocloudservice.com\/wordpress\/?p=61823"},"modified":"2024-11-21T15:38:11","modified_gmt":"2024-11-21T07:38:11","slug":"%e8%bf%98%e5%9c%a8%e4%b8%ba%e5%9f%ba%e5%9b%a0%e7%bb%84%e7%bb%84%e8%a3%85%e5%8f%91%e6%84%81%ef%bc%9f%e7%94%a8megahit%ef%bc%8c%e5%8f%aa%e9%9c%80%e7%ae%80%e5%8d%95%e5%87%a0%e6%ad%a5%ef%bc%8c%e8%ae%a9","status":"publish","type":"post","link":"http:\/\/www.biocloudservice.com\/wordpress\/?p=61823","title":{"rendered":"\u8fd8\u5728\u4e3a\u57fa\u56e0\u7ec4\u7ec4\u88c5\u53d1\u6101\uff1f\u7528MEGAHIT\uff0c\u53ea\u9700\u7b80\u5355\u51e0\u6b65\uff0c\u8ba9\u4f60\u7684\u57fa\u56e0\u7ec4\u7ec4\u88c5\u901f\u5ea6\u548c\u6548\u7387\u6210\u500d\u63d0\u5347\uff01"},"content":{"rendered":"\n
\"\"<\/figure>\n\n\n\n

\u540c\u5b66\u4eec\uff0c\u4eca\u5929\u5c0f\u5e08\u59b9\u8981\u5411\u5927\u5bb6\u4ecb\u7ecd\u4e00\u6b3e\u5728\u57fa\u56e0\u7ec4\u7ec4\u88c5\u9886\u57df\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u8f6f\u4ef6\u2014\u2014MEGAHIT\uff01MEGAHIT \u662f\u4e00\u4e2a\u4e13\u4e3a\u5927\u89c4\u6a21\u57fa\u56e0\u7ec4\u548c\u5b8f\u57fa\u56e0\u7ec4\u6570\u636e\u8bbe\u8ba1\u7684\u9ad8\u6548 de novo \u7ec4\u88c5\u5de5\u5177\u3002\u5b83\u7684\u51fa\u73b0\u8ba9\u5904\u7406\u6d77\u91cf\u6d4b\u5e8f\u6570\u636e\u53d8\u5f97\u66f4\u52a0\u5feb\u901f\u548c\u9ad8\u6548\uff0c\u5c24\u5176\u662f\u5728\u5904\u7406 Illumina \u77ed\u8bfb\u957f\u6570\u636e\u65f6\u8868\u73b0\u51fa\u8272\u3002\u901a\u8fc7 MEGAHIT\uff0c\u6211\u4eec\u80fd\u591f\u5feb\u901f\u7ec4\u88c5\u51fa\u57fa\u56e0\u7ec4\u5e8f\u5217\uff0c\u4e3a\u540e\u7eed\u7684\u529f\u80fd\u6ce8\u91ca\u3001\u6bd4\u8f83\u57fa\u56e0\u7ec4\u5b66\u5206\u6790\u7b49\u63d0\u4f9b\u91cd\u8981\u7684\u57fa\u7840\u6570\u636e\u3002\u7ec4\u88c5\u57fa\u56e0\u7ec4\u5bf9\u5c0f\u5e08\u59b9\u6765\u8bf4\u8f7b\u800c\u6613\u4e3e\uff0c\u8981\u662f\u540c\u5b66\u4eec\u9047\u5230\u4efb\u4f55\u751f\u4fe1\u95ee\u9898\uff0c\u968f\u65f6\u6765\u627e\u5c0f\u5e08\u59b9\u8ba8\u8bba\u54e6\uff01<\/strong><\/p>\n\n\n\n

\u5728\u672c\u6b21\u5b66\u4e60\u4e2d\uff0c\u6211\u4eec\u5c06\u4ece MEGAHIT \u7684\u5b89\u88c5\u5f00\u59cb\uff0c\u9010\u6b65\u5b66\u4e60\u5982\u4f55\u4f7f\u7528\u8fd9\u6b3e\u5de5\u5177\u8fdb\u884c\u57fa\u56e0\u7ec4\u7ec4\u88c5\uff0c\u5e76\u4e14\u7406\u89e3\u7ec4\u88c5\u7ed3\u679c\u7684\u89e3\u6790\u65b9\u6cd5\u3002\u901a\u8fc7\u5b9e\u9645\u793a\u4f8b\uff0c\u6211\u4eec\u5c06\u66f4\u52a0\u6df1\u5165\u5730\u4e86\u89e3\u57fa\u56e0\u7ec4\u7ec4\u88c5\u7684\u5de5\u4f5c\u6d41\u7a0b\uff0c\u638c\u63e1\u547d\u4ee4\u884c\u7684\u57fa\u672c\u4f7f\u7528\u548c\u6570\u636e\u5206\u6790\u7684\u6838\u5fc3\u6b65\u9aa4\u3002\u901a\u8fc7\u4eca\u5929\u7684\u5b66\u4e60\uff0c\u540c\u5b66\u4eec\u5c06\u80fd\u591f\u66f4\u52a0\u719f\u7ec3\u5730\u5e94\u7528 MEGAHIT\uff0c\u5728\u57fa\u56e0\u7ec4\u7ec4\u88c5\u548c\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u7684\u9886\u57df\u4e2d\u8fc8\u51fa\u575a\u5b9e\u7684\u4e00\u6b65\uff01\u63a5\u4e0b\u6765\uff0c\u8ba9\u6211\u4eec\u4e00\u8d77\u5f00\u542f MEGAHIT \u7684\u5b66\u4e60\u4e4b\u65c5\u5427\uff01<\/p>\n\n\n\n

\u672c\u6b21\u4ecb\u7ecd\u7684\u5de5\u5177\u9700\u8981\u5728\u670d\u52a1\u5668\u4e0a\u624d\u80fd\u6b63\u5e38\u8fd0\u884c\uff0c\u540c\u5b66\u4eec\u5982\u679c\u6ca1\u6709\u81ea\u5df1\u7684\u670d\u52a1\u5668\u6b22\u8fce\u8054\u7cfb\u6211\u4eec\u8fdb\u884c\u670d\u52a1\u5668\u79df\u8d41~<\/strong><\/strong><\/p>\n\n\n\n

MEGAHIT\u8f6f\u4ef6\u4ecb\u7ecd<\/p>\n\n\n\n

MEGAHIT \u662f\u4e00\u4e2a\u9ad8\u6548\u7684 de novo \u7ec4\u88c5\u5de5\u5177\uff0c\u4e3b\u8981\u7528\u4e8e\u5904\u7406\u5927\u89c4\u6a21\u57fa\u56e0\u7ec4\u548c\u5b8f\u57fa\u56e0\u7ec4\u6570\u636e\u3002\u5b83\u91c7\u7528 de Bruijn \u56fe\u7b97\u6cd5\uff0c\u4e13\u4e3a\u5904\u7406\u6d77\u91cf\u6d4b\u5e8f\u6570\u636e\u800c\u8bbe\u8ba1\uff0c\u5c24\u5176\u9002\u5408 Illumina \u77ed\u8bfb\u957f\u6570\u636e\u7684\u5feb\u901f\u7ec4\u88c5\u3002MEGAHIT \u5177\u6709\u4f4e\u5185\u5b58\u9700\u6c42\u548c\u9ad8\u901f\u5ea6\u7684\u4f18\u52bf\uff0c\u80fd\u591f\u5728\u5904\u7406\u6570\u767e\u4e07\u5230\u6570\u5341\u4ebf\u4e2a\u8bfb\u53d6\u65f6\u4f9d\u7136\u4fdd\u6301\u51fa\u8272\u7684\u6027\u80fd\u3002\u8be5\u5de5\u5177\u4f7f\u7528\u7b80\u5355\uff0c\u63d0\u4f9b\u7528\u6237\u53cb\u597d\u7684\u547d\u4ee4\u884c\u754c\u9762\uff0c\u5e76\u652f\u6301\u591a\u79cd\u53c2\u6570\u8c03\u6574\uff0c\u5141\u8bb8\u7528\u6237\u6839\u636e\u5177\u4f53\u9700\u6c42\u4f18\u5316\u7ec4\u88c5\u8fc7\u7a0b\u3002\u5b83\u751f\u6210\u7684\u7ec4\u88c5\u7ed3\u679c\u53ef\u4ee5\u7528\u4e8e\u540e\u7eed\u7684\u751f\u6001\u5b66\u3001\u73af\u5883\u57fa\u56e0\u7ec4\u5b66\u7b49\u9886\u57df\u7684\u590d\u6742\u6570\u636e\u5206\u6790\uff0c\u662f\u7814\u7a76\u5fae\u751f\u7269\u7ec4\u7684\u91cd\u8981\u5de5\u5177\u3002<\/p>\n\n\n\n

MEGAHIT\u8f6f\u4ef6\u5b89\u88c5<\/p>\n\n\n\n

MEGAHIT\u5fc5\u987b\u8981\u5728\u670d\u52a1\u5668\u4e0a\u8fd0\u884c\uff0c\u9700\u8981\u540c\u5b66\u4eec\u6709\u4e00\u4e9bLinux\u7cfb\u7edf\u7684\u57fa\u7840\u77e5\u8bc6\uff0c\u5982\u679c\u6ca1\u6709\u4e5f\u4e0d\u7528\u62c5\u5fc3\uff0c\u5c0f\u5e08\u59b9\u5e26\u4f60\u4e00\u6b65\u4e00\u6b65\u6765\uff0c\u8ddf\u7d27\u5c0f\u5e08\u59b9\u7684\u6b65\u4f10\uff0c\u8ba9\u6211\u4eec\u4eceMEGAHIT\u7684\u5b89\u88c5\u5f00\u59cb\uff0c\u6162\u6162\u5b66\u4e60\u5982\u4f55\u4f7f\u7528\u8fd9\u6b3e\u5f3a\u5927\u7684\u5de5\u5177\u5427\u3002<\/p>\n\n\n\n

\u9700\u8981\u7684\u7cfb\u7edf\uff1aLinux\u7cfb\u7edf\uff0c\u9700\u8981\u7684\u8f6f\u4ef6\u652f\u6301\uff1aconda<\/p>\n\n\n\n

\u4e3a\u4e86\u907f\u514d\u6211\u4eec\u73b0\u5728\u7684\u7cfb\u7edf\u73af\u5883\u4e0d\u7b26\u5408MEGAHIT\u8f6f\u4ef6\u7684\u8981\u6c42\uff0c\u6240\u4ee5\u6211\u4eec\u9700\u8981\u4e3aMEGAHIT\u5b89\u88c5\u4e00\u4e2a\u865a\u62df\u7684\u5de5\u4f5c\u73af\u5883\uff0c\u5728\u4e0d\u66f4\u6539\u73b0\u5728\u7cfb\u7edf\u73af\u5883\u7684\u524d\u63d0\u4e0b\uff0c\u5b89\u88c5MEGAHIT\uff0c\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

conda create –name megahit # \u521b\u5efa\u4e00\u4e2aMEGAHIT\u73af\u5883<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u9047\u5230\u56fe\u4e0a\u63d0\u793a\uff0c\u8f93\u5165y\u5373\u53ef<\/p>\n\n\n\n

\u521b\u5efa\u5b8c\u73af\u5883\u540e\uff0c\u6211\u4eec\u6fc0\u6d3b\u73af\u5883\uff0c\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

conda activate megahit # \u6fc0\u6d3bMEGAHIT\u73af\u5883<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u663e\u793a\uff08megahit\uff09\u5219\u8868\u660e\u6211\u4eec\u5df2\u7ecf\u6210\u529f\u521b\u5efa\u5e76\u4e14\u6fc0\u6d3b\u4e86MEGAHIT\u73af\u5883\uff0c\u63a5\u7740\u6211\u4eec\u5c31\u53ef\u4ee5\u5728\u8be5\u73af\u5883\u4e0b\u5b89\u88c5MEGAHIT\u4e86\uff0c\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

conda install -c bioconda megahit # \u5728conda\u73af\u5883\u4e2d\u5b89\u88c5megahit<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u9047\u5230\u56fe\u4e0a\u63d0\u793a\uff0c\u8f93\u5165y\u5373\u53ef<\/p>\n\n\n\n

\u8010\u5fc3\u7b49\u5f85\u5b89\u88c5\u5b8c\u6210\u540e\uff0c\u6211\u4eec\u53ef\u4ee5\u8f93\u5165\u4ee5\u4e0b\u547d\u4ee4\u6d4b\u8bd5\u662f\u5426\u5b89\u88c5\u6210\u529f\u3002<\/p>\n\n\n\n

megahit -h #\u5524\u9192MEGAHIT \u53c2\u8003\u624b\u518c<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u5982\u679c\u663e\u793a\u5982\u56fe\u6240\u793a\u7684v1.2.9\u7248\u672c\u63d0\u793a\uff0c\u5c31\u8868\u660e\u5df2\u7ecf\u6210\u529f\u5b89\u88c5\u4e86MEGAHIT\u7a0b\u5e8f\u3002<\/p>\n\n\n\n

\u4f7f\u7528MEGAHIT\u8fdb\u884c\u4e8c\u4ee3\u6d4b\u5e8f\u7ed3\u679c de novo\u7ec4\u88c5<\/p>\n\n\n\n

MEGAHIT\u4e3b\u8981\u547d\u4ee4<\/strong><\/strong><\/p>\n\n\n\n

MEGAHIT \u7684\u4e3b\u8981\u547d\u4ee4\u884c\u8bed\u53e5\u975e\u5e38\u7b80\u6d01\uff0c\u901a\u8fc7\u63d0\u4f9b\u8f93\u5165\u6587\u4ef6\u548c\u4e00\u4e9b\u53c2\u6570\uff0c\u5c31\u53ef\u4ee5\u542f\u52a8\u7ec4\u88c5\u4efb\u52a1\u3002\u4ee5\u4e0b\u662f MEGAHIT \u5e38\u7528\u7684\u547d\u4ee4\u8bed\u53e5\u53ca\u5176\u4e3b\u8981\u53c2\u6570\u7684\u4ecb\u7ecd\uff1a<\/p>\n\n\n\n

1. \u57fa\u672c\u547d\u4ee4\u683c\u5f0f<\/strong><\/strong><\/p>\n\n\n\n

megahit -r <reads.fastq> -o <output_dir><\/p>\n\n\n\n

-r\uff1a\u7528\u4e8e\u5355\u7aef\uff08single-end\uff09\u6d4b\u5e8f\u6570\u636e\u7684\u8f93\u5165\u6587\u4ef6\uff0c\u652f\u6301 fastq \u683c\u5f0f\u3002<\/p>\n\n\n\n

-o\uff1a\u6307\u5b9a\u8f93\u51fa\u76ee\u5f55\uff0cMEGAHIT \u4f1a\u5c06\u7ec4\u88c5\u7ed3\u679c\u4fdd\u5b58\u5230\u8be5\u76ee\u5f55\u3002<\/p>\n\n\n\n

2. \u53cc\u7aef\u6d4b\u5e8f\u6570\u636e\u7684\u7ec4\u88c5<\/strong><\/strong><\/p>\n\n\n\n

megahit -1 <reads_1.fastq> -2 <reads_2.fastq> -o <output_dir><\/p>\n\n\n\n

-1 \u548c -2\uff1a\u5206\u522b\u6307\u5b9a\u53cc\u7aef\uff08paired-end\uff09\u6d4b\u5e8f\u6570\u636e\u7684\u524d\u5411\u548c\u53cd\u5411\u8bfb\u6bb5\u3002<\/p>\n\n\n\n

3. \u8bbe\u5b9a\u7ebf\u7a0b\u6570\u548c\u5185\u5b58\u5927\u5c0f<\/strong><\/strong><\/p>\n\n\n\n

megahit -1 <reads_1.fastq> -2 <reads_2.fastq> -t <num_threads> -m <memory_size> -o <output_dir><\/p>\n\n\n\n

-t\uff1a\u6307\u5b9a\u4f7f\u7528\u7684\u7ebf\u7a0b\u6570\uff08CPU\u6838\u6570\uff09\uff0c\u9ed8\u8ba4\u503c\u4e3a4\uff0c\u6839\u636e\u8ba1\u7b97\u8d44\u6e90\u53ef\u4ee5\u8c03\u6574\u3002<\/p>\n\n\n\n

-m\uff1a\u6307\u5b9a\u6700\u5927\u5185\u5b58\u4f7f\u7528\u91cf\uff0c\u4ee5 GB \u4e3a\u5355\u4f4d\u3002<\/p>\n\n\n\n

\u4f7f\u7528MEGAHIT\u8fdb\u884c\u6d4b\u5e8f\u6570\u636ede novo \u7ec4\u88c5<\/strong><\/strong><\/p>\n\n\n\n

\u5728\u672c\u6587\u4e2d\uff0c\u5c0f\u5e08\u59b9\u4e3b\u8981\u5411\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e0b\u53cc\u7aef\u6d4b\u5e8f\u6570\u636e\u7ec4\u88c5\u7684\u547d\u4ee4\u8bed\u6cd5\uff0c \u6211\u4eec\u5c06\u4f7f\u7528MEGAHIT\u5f00\u53d1\u8005\u53d1\u5e03\u5728Github\u5e73\u53f0\u4e0a\u7684\u6d4b\u8bd5\u6570\u636e\u4f5c\u4e3a\u793a\u4f8b\u6570\u636e\u8fdb\u884c\u6f14\u793a\uff0c\u76f8\u5173\u6570\u636e\u53ef\u4ee5\u5728\u516c\u4f17\u53f7\u4e0a\u83b7\u53d6\u3002\u628a\u793a\u4f8b\u6587\u4ef6\u7f6e\u4e8e\u5f53\u524d\u76ee\u5f55\uff0c\u8fdb\u884cde novo \u7ec4\u88c5\u7684\u76f8\u5173\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

ls #\u67e5\u770b\u5f53\u524d\u76ee\u5f55\u6587\u4ef6<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u663e\u793a\u5982\u4e0a\u56fe\uff0c\u5219\u8868\u793a\u6211\u4eec\u5df2\u7ecf\u51c6\u5907\u597d\u4e86\u8fdb\u884cde novo \u7ec4\u88c5\u6240\u9700\u8981\u7684\u793a\u4f8b\u6570\u636e\u3002<\/p>\n\n\n\n

\u63a5\u7740\uff0c\u6211\u4eec\u4f7f\u7528MEGAHIT\u7684\u53cc\u7aef\u6d4b\u5e8f\u6570\u636e\u7ec4\u88c5\u547d\u4ee4\u6765\u8fdb\u884cde novo \u7ec4\u88c5\uff0c\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

megahit -1 r3_1.fa -2 r3_2.fa -t 32 -o result # \u542f\u52a8MEGAHIT\u8fdb\u884c\u57fa\u56e0\u7ec4\u7ec4\u88c5\uff0c\u6307\u5b9a\u524d\u5411\u548c\u53cd\u5411\u8bfb\u6bb5\u6587\u4ef6\uff0c\u4f7f\u752832\u4e2a\u7ebf\u7a0b\uff0c\u7ed3\u679c\u8f93\u51fa\u5230’result’\u76ee\u5f55<\/p>\n\n\n\n

\u7ed3\u679c\u5982\u4e0b\u56fe<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u663e\u793a\u201cALL DONE\u201d\u5219\u8868\u793a\u7ec4\u88c5\u5b8c\u6210\uff0c\u4f1a\u5728\u5f53\u524d\u76ee\u5f55\u751f\u6210\u4e00\u4e2a\u201cresult\u201d\u6587\u4ef6\u5939\u3002<\/p>\n\n\n\n

de novo \u7ec4\u88c5\u7ed3\u679c\u67e5\u770b\u548c\u89e3\u6790<\/strong><\/strong><\/p>\n\n\n\n

\u7ecf\u8fc7\u4ee5\u4e0a\u7684\u6b65\u9aa4\u6211\u4eec\u5df2\u7ecf\u6210\u529f\u83b7\u5f97\u4e86\u793a\u4f8b\u6570\u636e\u7ec4\u88c5\u7684\u7ed3\u679c\uff0c\u63a5\u4e0b\u6765\u6211\u4eec\u53ef\u4ee5\u8fdb\u5165\u201cresult\u201d\u6587\u4ef6\u5939\u5bf9\u7ed3\u679c\u8fdb\u884c\u67e5\u770b\u548c\u89e3\u6790\uff0c\u76f8\u5173\u7684\u547d\u4ee4\u5982\u4e0b\uff1a<\/p>\n\n\n\n

cd result\/ # \u8fdb\u5165\u201cresult\u201d\u6587\u4ef6\u5939<\/p>\n\n\n\n

ls #\u67e5\u770b\u5f53\u524d\u76ee\u5f55\u6587\u4ef6<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u5176\u4e2dfinal.contigs.fa\u5373\u4e3a\u6700\u7ec8\u7ec4\u88c5\u7684\u7ed3\u679c\uff0c\u6211\u4eec\u53ef\u4ee5\u4f7f\u7528cat\u547d\u4ee4\u8fdb\u884c\u67e5\u770b\u3002<\/p>\n\n\n\n

cat final.contigs.fa # \u67e5\u770bfinal.contigs.fa\u6587\u4ef6\u5185\u5bb9<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u7531\u4e0a\u56fe\u53ef\u77e5\uff0c\u6807\u8bc6\u7b26\u4e3ak59_0 \uff0c\u662f\u8be5 Contig \u7684\u540d\u79f0\u6216\u7f16\u53f7\uff0c\u901a\u5e38\u8868\u793a\u5176\u5728\u7ec4\u88c5\u8fc7\u7a0b\u4e2d\u7684\u751f\u6210\u4fe1\u606f\u3002flag=1 \u8868\u793a\u8be5 Contig \u7684\u7ec4\u88c5\u72b6\u6001\u6216\u8d28\u91cf\u63a7\u5236\u6807\u5fd7\uff0c\u5177\u4f53\u542b\u4e49\u9700\u53c2\u8003\u76f8\u5173\u6587\u6863\u3002\u591a\u91cd\u6027\uff1amulti=7.5929 \u6307\u793a\u5728\u7ec4\u88c5\u8fc7\u7a0b\u4e2d\uff0c\u6b64 Contig \u53ef\u80fd\u4e0e\u591a\u4e2a\u5176\u4ed6 Contig \u6709\u91cd\u53e0\uff0c\u6570\u503c\u8868\u793a\u591a\u91cd\u6027\u7a0b\u5ea6\u3002\u957f\u5ea6\uff1alen=1221 \u8868\u793a\u8be5 Contig \u7684\u957f\u5ea6\u4e3a1221\u4e2a\u78b1\u57fa\u5bf9\uff08bp\uff09\u3002\u540e\u9762\u7684\u78b1\u57fa\u5e8f\u5217\u5219\u662f\u8be5 Contig \u7684\u5177\u4f53 DNA \u5e8f\u5217\uff0c\u53ef\u4ee5\u7528\u4e8e\u8fdb\u4e00\u6b65\u7684\u5206\u6790\uff0c\u5982\u57fa\u56e0\u9884\u6d4b\u3001\u529f\u80fd\u6ce8\u91ca\u6216\u6bd4\u8f83\u57fa\u56e0\u7ec4\u5b66\u7b49\u3002<\/p>\n\n\n\n

\u4ee5\u4e0a\u5c31\u662f\u5bf9MEGAHIT\u8f6f\u4ef6\u7684\u5168\u90e8\u4ecb\u7ecd\u4e86\u3002\u901a\u8fc7\u672c\u6587\uff0c\u6211\u4eec\u4e86\u89e3\u4e86MEGAHIT\u4f5c\u4e3a\u4e00\u4e2a\u9ad8\u6548\u7684de novo\u7ec4\u88c5\u5de5\u5177\uff0c\u5728\u5904\u7406\u5927\u89c4\u6a21\u57fa\u56e0\u7ec4\u548c\u5b8f\u57fa\u56e0\u7ec4\u6570\u636e\u65f6\u7684\u5e94\u7528\u3002\u5e76\u4e14\u5b66\u4e60\u4e86 MEGAHIT \u7684\u5b89\u88c5\u6b65\u9aa4\u53ca\u5176\u5e38\u7528\u7684\u547d\u4ee4\u884c\u8bed\u6cd5\uff0c\u901a\u8fc7\u4f7f\u7528\u793a\u4f8b\u6570\u636e\u8fdb\u884c\u4e86 de novo \u7ec4\u88c5\u3002\u5e0c\u671b\u5927\u5bb6\u5728\u5b66\u4e60\u548c\u4f7f\u7528 MEGAHIT \u65f6\uff0c\u80fd\u591f\u719f\u7ec3\u638c\u63e1\u5176\u57fa\u672c\u64cd\u4f5c\u7684\u540c\u65f6\uff0c\u901a\u8fc7\u4e0d\u65ad\u5b9e\u8df5\uff0c\u63d0\u5347\u5728\u57fa\u56e0\u7ec4\u7ec4\u88c5\u548c\u5206\u6790\u4e2d\u7684\u6280\u80fd\uff0c\u4e3a\u751f\u7269\u4fe1\u606f\u5b66\u7814\u7a76\u505a\u51fa\u8d21\u732e\u3002<\/p>\n\n\n\n

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