{"id":65783,"date":"2026-10-10T11:06:52","date_gmt":"2026-10-10T03:06:52","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/bulk-rnaseq-ferroptosis-score-method\/"},"modified":"2026-10-10T11:06:52","modified_gmt":"2026-10-10T03:06:52","slug":"bulk-rnaseq-ferroptosis-score-method","status":"publish","type":"ftc_question","link":"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/bulk-rnaseq-ferroptosis-score-method\/","title":{"rendered":"bulk RNA-seq \u94c1\u6b7b\u4ea1\u8bc4\u5206\u8be5\u9009 GSVA\u3001ssGSEA \u8fd8\u662f\u5176\u4ed6\u65b9\u6cd5\uff1f"},"content":{"rendered":"<p class=\"ftc-answer-first\"><strong>\u5148\u770b\u7ed3\u8bba\uff1a<\/strong>\u6ca1\u6709\u4e00\u79cd\u8bc4\u5206\u65b9\u6cd5\u9002\u5408\u6240\u6709\u6570\u636e\u3002\u5148\u786e\u8ba4\u8868\u8fbe\u503c\u3001\u6837\u672c\u6570\u91cf\u3001\u57fa\u56e0\u96c6\u7248\u672c\u548c\u7814\u7a76\u76ee\u6807\uff0c\u518d\u9009\u65b9\u6cd5\uff1b\u540c\u4e00\u9879\u76ee\u4e0d\u8981\u5728\u770b\u5230\u7ed3\u679c\u540e\u53cd\u590d\u6362\u7b97\u6cd5\u6311\u663e\u8457\u7ed3\u679c\u3002<\/p>\n<h2>\u9002\u7528\u60c5\u51b5<\/h2>\n<p>\u5df2\u7ecf\u6709\u82af\u7247\u6216 bulk RNA-seq \u7684\u57fa\u56e0\u00d7\u6837\u672c\u8868\u8fbe\u77e9\u9635\uff0c\u5e0c\u671b\u5f97\u5230\u6bcf\u4e2a\u6837\u672c\u7684\u94c1\u6b7b\u4ea1\u76f8\u5173\u5206\u6570\u3002<\/p>\n<h2>\u5efa\u8bae\u6309\u8fd9\u4e2a\u987a\u5e8f\u68c0\u67e5<\/h2>\n<ol>\n<li>\u5148\u786e\u8ba4\u8f93\u5165\u662f\u82af\u7247\u6807\u51c6\u5316\u8868\u8fbe\u3001TPM\/CPM \u6216\u7ecf\u8fc7\u5408\u9002\u53d8\u6362\u7684\u8868\u8fbe\u77e9\u9635\uff1b\u539f\u59cb count \u4e0d\u5e94\u4e0d\u52a0\u8bf4\u660e\u5730\u76f4\u63a5\u5f53\u6210\u8fde\u7eed\u8868\u8fbe\u503c\u4f7f\u7528\u3002<\/li>\n<li>\u56fa\u5b9a\u94c1\u6b7b\u4ea1\u57fa\u56e0\u96c6\u7684\u6765\u6e90\u3001\u7248\u672c\u3001\u7269\u79cd\u548c\u7b5b\u9009\u53e3\u5f84\uff0c\u8bb0\u5f55\u5b9e\u9645\u547d\u4e2d\u7684\u57fa\u56e0\u6570\u91cf\u3002<\/li>\n<li>\u5982\u679c\u76ee\u6807\u662f\u6837\u672c\u95f4\u8fde\u7eed\u8bc4\u5206\uff0c\u53ef\u6bd4\u8f83 GSVA \u4e0e ssGSEA \u7684\u7a33\u5b9a\u6027\uff1b\u5982\u679c\u76ee\u6807\u662f\u7ec4\u95f4\u5dee\u5f02\uff0c\u4ecd\u8981\u56de\u5230\u7814\u7a76\u8bbe\u8ba1\u3001\u6279\u6b21\u548c\u534f\u53d8\u91cf\u3002<\/li>\n<li>\u5148\u5728\u9884\u5148\u786e\u5b9a\u7684\u4e3b\u8981\u65b9\u6cd5\u4e0a\u5b8c\u6210\u5206\u6790\uff0c\u518d\u628a\u53e6\u4e00\u79cd\u65b9\u6cd5\u4f5c\u4e3a\u654f\u611f\u6027\u5206\u6790\uff0c\u4e0d\u4ee5\u54ea\u4e2a\u66f4\u663e\u8457\u4f5c\u4e3a\u9009\u62e9\u4f9d\u636e\u3002<\/li>\n<li>\u7528\u5916\u90e8\u961f\u5217\u6216\u72ec\u7acb\u8bc1\u636e\u9a8c\u8bc1\u65b9\u5411\uff0c\u4e0d\u628a\u4e00\u4e2a\u5206\u6570\u76f4\u63a5\u7b49\u540c\u4e8e\u94c1\u6b7b\u4ea1\u5df2\u7ecf\u53d1\u751f\u3002<\/li>\n<\/ol>\n<h2>\u4e0d\u80fd\u4ece\u5f53\u524d\u7ed3\u679c\u76f4\u63a5\u5f97\u51fa\u4ec0\u4e48<\/h2>\n<p>\u8f6c\u5f55\u7ec4\u8bc4\u5206\u53cd\u6620\u7684\u662f\u57fa\u56e0\u96c6\u8868\u8fbe\u6a21\u5f0f\uff0c\u4e0d\u662f\u8102\u8d28\u8fc7\u6c27\u5316\u3001\u94c1\u4f9d\u8d56\u6027\u7ec6\u80de\u6b7b\u4ea1\u6216\u836f\u7406\u5b66\u6551\u63f4\u5b9e\u9a8c\u7684\u66ff\u4ee3\u8bc1\u636e\u3002\u8de8\u5e73\u53f0\u5206\u6570\u901a\u5e38\u4e0d\u80fd\u76f4\u63a5\u6bd4\u8f83\u7edd\u5bf9\u503c\u3002<\/p>\n<h2>\u4f9d\u636e\u4e0e\u7248\u672c<\/h2>\n<ul class=\"ftc-source-list\">\n<li><a href=\"https:\/\/bioconductor.org\/packages\/release\/bioc\/manuals\/GSVA\/man\/GSVA.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Bioconductor GSVA \u8f6f\u4ef6\u624b\u518c<\/a><span>GSVA 2.2.1 \u624b\u518c<\/span><\/li>\n<li><a href=\"https:\/\/academic.oup.com\/nar\/article\/54\/D1\/D572\/8307356\" target=\"_blank\" rel=\"noopener noreferrer\">FerrDb V3\uff1a\u8c03\u63a7\u6027\u7ec6\u80de\u6b7b\u4ea1\u6570\u636e\u5e93<\/a><span>FerrDb V3<\/span><\/li>\n<\/ul>\n<p class=\"ftc-verification\">\u6700\u540e\u6838\u9a8c\uff1a2026-10-10\uff5c\u8d44\u6599\u6838\u9a8c\uff1a\u516c\u5f00\u6765\u6e90\u6838\u5bf9<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6ca1\u6709\u4e00\u79cd\u8bc4\u5206\u65b9\u6cd5\u9002\u5408\u6240\u6709\u6570\u636e\u3002\u5148\u786e\u8ba4\u8868\u8fbe\u503c\u3001\u6837\u672c\u6570\u91cf\u3001\u57fa\u56e0\u96c6\u7248\u672c\u548c\u7814\u7a76\u76ee\u6807\uff0c\u518d\u9009\u65b9\u6cd5\uff1b\u540c\u4e00\u9879\u76ee\u4e0d\u8981\u5728\u770b\u5230\u7ed3\u679c\u540e\u53cd\u590d\u6362\u7b97\u6cd5\u6311\u663e\u8457\u7ed3\u679c\u3002<\/p>\n","protected":false},"template":"","class_list":["post-65783","ftc_question","type-ftc_question","status-publish","hentry"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question\/65783","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/ftc_question"}],"version-history":[{"count":0,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question\/65783\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=65783"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}